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Nontuberculosis mycobacterium

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https://www.readbyqxmd.com/read/28043629/nontuberculosis-mycobacteria-isolation-from-clinical-samples-in-iraq
#1
Shatha T Ahmed, Ahmed A Alsudani, Ruqaya M Ali, Batool A Shihab, Sana A Hussein
OBJECTIVE/BACKGROUND: Nontuberculosis mycobacteria (NTM), defined as any mycobacterial strain other than Mycobacterium tuberculosis complex, are a diverse group of pathogens that cause a substantive, but often unappreciated worldwide burden of illness. NTM cause illness similar to M. tuberculosis, but generally do not respond to classic tuberculosis (TB) drug regimens. Here, we evaluated GenoType Mycobacterium CM/AS (Hain Lifesciences) for rapid identification of NTM and compared its results with those of other biochemical tests...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043563/molecular-drug-susceptibility-testing-against-the-first-line-rifampin-and-isoniazid-and-second-line-ciprofloxacin-amikacin-and-kanamycin-treatment-in-different-subtypes-of-mycobacterium-simiae
#2
Parissa Farnia, Donya Malekshahian, Payam Tabarsi, Shima Seif, Ali Akbar Velayati
OBJECTIVE BACKGROUND: Mycobacterium simiae has been identified as the most prevalent slow growing mycobacteria that has been isolated among Iranian patients. Due to an importance of molecular approaches in the treatment of nontuberculosis mycobacteria, this study aimed to evaluate molecular drug susceptibility testing against the first-line (rifampin and isoniazid) and second-line (ciprofloxacin-amikacin and kanamycin) treatment in different subtypes of M. simiae. METHODS: The study involved all patients presenting to our referral tuberculosis center from March 2014 to August 2016, with confirmation of M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043561/identification-of-different-subtypes-of-rapid-growing-atypical-mycobacterium-from-water-and-soil-sources-using-pcr-rflp-using-hsp65-and-rrna-16s-23s-genes
#3
Mohammad Varahram, Parissa Farnia, Shima Saif, Mehran Marashian, Poopak Farnia, Jaladein Ghanavi, Ali Akbar Velayati
OBJECTIVE/BACKGROUND: Nontuberculosis mycobacteria (NTM) are a diverse group of microorganisms that cause a variety of diseases in humans including skin, respiratory, and gastrointestinal tract infection. Generally, NTM are classified into two categories: rapid (<7days) and slow growing (>7days). In this study, we aimed to investigate NTM frequency and prevalence in environmental samples. Additionally, we tried to identify various subtypes of isolated rapid growing mycobacteria (RGM)...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043560/the-importance-of-single-nucleotide-polymorphisms-in-interferon-gamma-receptor-1-gene-in-pulmonary-patients-infected-with-rapid-grower-mycobacterium
#4
Poopak Farnia, Jalaledin Ghanavi, Payam Tabasri, Shima Saif, Ali Akbar Velayati
OBJECTIVE/BACKGROUND: Interferon gamma (IFN-γ) plays a key role in protective immune response against Mycobacterial infection. IFN-γ excretes its antimycobacterial effectors mechanisms by activation of macrophages and dendritic cells via interaction with its receptor complex, that is, a ligand-binding subunit [IFN-γ receptor (IFNGR)1] and an accessory subunit (IFNGR2) on the cell surface. It has been shown that individuals with complete or partial IFNGR1 receptor deficiency are highly susceptible to infection by nontuberculous mycobacteria (NTM), Mycobacterium tuberculosis, and some Salmonella species...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043546/molecular-identification-of-mycobacterium-tuberculosis-complex-by-region-of-differentiation-typing-and-polymerase-chain-reaction-restriction-fragment-length-polymorphism-method
#5
Seydeh Zeinab Mirzaki, Nader Mosavari, Razieh Nazari, Morteza Akbarian
Tuberculosis (TB) is one of the most common zoonotic infectious diseases in the world. Identification of Mycobacterium isolates is essential for proper treatment of TB. The aim of this study was to identify Mycobacterium isolates collected from TB patients in Alborz Province, Iran, by region of differentiation (RD)-typing. Fifty samples from tuberculosis patients were cultured in pyruvate and glycerinated Lowenstein-Jensen medium. DNA was extracted from the isolates by the van Solingen method and subjected to polymerase chain reaction (PCR)-16SrRNA, PCR-IS6110, and RD-typing with primers RD1, RD4, RD9, and RD12, respectively...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/27931681/mycobacterial-contamination-of-bronchoscopes-challenges-and-possible-solutions-in-low-resource-settings
#6
Dania Khalid Saeed, Sadia Shakoor, Seema Irfan, Rumina Hasan
The use of bronchoscopes has increased in tuberculosis (TB) diagnostics to circumvent the diagnostic challenges that are associated with low sputum volume and smear-negative TB. In healthcare facilities situated in low income countries that have a high burden of TB, adequate decontamination of bronchoscopes is a challenge and often overlooked to save on time and costs. This amplifies the risk of outbreaks and pseudo-outbreaks due to Mycobacterium tuberculosis and nontuberculosis mycobacteria. In this minireview, we review published literature of contaminated bronchoscopes causing pseudo-outbreaks of M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/27790302/urinary-mycobacterium-avium-presenting-as-sterile-pyuria
#7
Kai Yang, Mary Samplaski, Tony Mazzulli, Kirk Lo, Ethan Grober, Keith Allen Jarvi
A 65-year-old healthy woman presented with persistent, asymptomatic sterile pyuria detected by her family physician. While she did not have symptoms, the patient recounts that she has had cloudy urine for years. Cultures of the urine for bacteria showed no growth and no fungi were identified. First-morning urine samples were sent for both tuberculosis and nontuberculosis mycobacterium species testing. The culture grew genotypically identified Mycobaterium avium complex (MAC). Mantoux skin testing was positive...
May 2016: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/27231376/draft-genome-sequence-of-mycobacterium-interjectum-strain-atcc-51457t
#8
Anthony Levasseur, Shady Asmar, Catherine Robert, Michel Drancourt
Mycobacterium interjectum is a nontuberculosis species rarely responsible for human infection. The draft genome of M. interjectum ATCC 51457(T) comprises 5,927,979 bp, exhibiting 67.91% G+C content, 5,314 protein-coding genes, and 51 predicted RNA genes.
2016: Genome Announcements
https://www.readbyqxmd.com/read/26922731/mycobacterium-chelonae-abscessus-complex-infection-after-flatfoot-reconstruction
#9
Jessica Lickiss, Ansgar Olsen, Jay D Ryan
Mycobacterium infections involving tuberculosis in the foot are rare but cases are readily available in published studies. Atypical or nontuberculosis mycobacterium foot infections are rare, especially those involving the soft tissue and bone, and have been infrequently reported. To date, no case reports involving atypical mycobacterium infection after elective foot surgery have been reported. We present the case of a 49-year-old female who underwent flatfoot reconstruction, with a resultant Mycobacterium chelonae-abscessus infection and osteomyelitis postoperatively...
November 2016: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/26586900/draft-genome-sequence-of-mycobacterium-mucogenicum-strain-csur-p2099
#10
Shady Asmar, Nicolás Rascovan, Catherine Robert, Michel Drancourt
Mycobacterium mucogenicum is a rapid-growing, nontuberculosis Mycobacterium species. The draft genome of M. mucogenicum CSUR P2099 comprises 6,210,127 bp exhibiting a 67.2% G+C content, 6,003 protein-coding genes, and 91 predicted RNA genes.
2015: Genome Announcements
https://www.readbyqxmd.com/read/26543131/draft-genome-sequence-of-mycobacterium-neworleansense-strain-atcc-49404t
#11
Shady Asmar, Catherine Robert, Olivier Croce, Aurelia Caputo, Michel Drancourt
Mycobacterium neworleansense is a rapid growing nontuberculosis species belonging to the Mycobacterium fortuitum complex. The draft genome of M. neworleansense ATCC 49404(T) comprises 6,287,317 bp exhibiting a 66.85% G+C content, 5,997 protein-coding genes, and 89 predicted RNA genes.
2015: Genome Announcements
https://www.readbyqxmd.com/read/26543113/draft-genome-sequence-of-mycobacterium-peregrinum-strain-csur-p2098
#12
Shady Asmar, Nicolás Rascovan, Catherine Robert, Michel Drancourt
Mycobacterium peregrinum is a nonpigmented rapid growing nontuberculosis species belonging to the Mycobacterium fortuitum group. The draft genome of M. peregrinum type I CSUR P2098 comprises 7,109,836 bp exhibiting a 66.23% G+C content, 6,894 protein-coding genes, and 100 predicted RNA genes. Its genome analysis suggests this species differs from Mycobacterium senegalense.
2015: Genome Announcements
https://www.readbyqxmd.com/read/26380237/molecular-typing-of-iranian-mycobacteria-isolates-by-polymerase-chain-reaction-restriction-fragment-length-polymorphism-analysis-of-360-bp-rpob-gene
#13
Shima Hadifar, Sharareh Moghim, Hossein Fazeli, Hajieh GhasemianSafaei, Seyed Asghar Havaei, Fariba Farid, Bahram Nasr Esfahani
BACKGROUND: Diagnosis and typing of Mycobacterium genus provides basic tools for investigating the epidemiology and pathogenesis of this group of bacteria. Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) is an accurate method providing diagnosis and typing of species of mycobacteria. The present study is conducted by the purpose of determining restriction fragment profiles of common types of mycobacteria by PRA method of rpoB gene in this geographical region...
2015: Advanced Biomedical Research
https://www.readbyqxmd.com/read/26251499/draft-genome-sequence-of-mycobacterium-bohemicum-strain-dsm-44277t
#14
Shady Asmar, Michael Phelippeau, Catherine Robert, Olivier Croce, Michel Drancourt
The Mycobacterium bohemicum strain is a nontuberculosis species mainly responsible for pediatric cervical lymphadenitis. The draft genome of M. bohemicum DSM 44277(T) comprises 5,097,190 bp exhibiting a 68.64% G+C content, 4,840 protein-coding genes, and 75 predicted RNA genes.
2015: Genome Announcements
https://www.readbyqxmd.com/read/26194558/identification-and-application-of-ssdna-aptamers-against-h%C3%A2-%C3%A2-rv-in-the-detection-of-mycobacterium-tuberculosis
#15
Rusitanmujiang Aimaiti, Lianhua Qin, Ting Cao, Hua Yang, Jie Wang, Junmei Lu, Xiaochen Huang, Zhongyi Hu
Microscopy of direct smear with the Ziehl-Neelsen stain is still broadly used in tuberculosis diagnosis. However, this method suffers from low specificity and is difficult to distinguish Mycobacterium tuberculosis (MTB) from nontuberculosis mycobacterial (NTM), since all mycobacterial species are positive in Ziehl-Neelsen stain. In this study, we utilized whole cell SELEX to obtain species-specific aptamers for increasing the specificity of MTB detection. Whole cell SELEX was performed in MTB reference strain H37Rv by two selection processes based on enzyme-linked plate or Eppendorf tube, respectively...
November 2015: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26064950/epidemiological-characterization-of-drug-resistance-among-mycobacterium-tuberculosis-isolated-from-patients-in-northeast-of-iran-during-2012-2013
#16
Ashraf Tavanaee Sani, Abolfazl Shakiba, Maryam Salehi, Hamid Reza Bahrami Taghanaki, Seiedeh Fatemeh Ayati Fard, Kiarash Ghazvini
INTRODUCTION: Tuberculosis is still one of the most important health problems in developing countries and increasing drug resistance is the main concern for its treatment. This study was designed to characterize the drug resistant Mycobacterium tuberculosis isolated from patients suffering from pulmonary tuberculosis in northeast of Iran. METHOD: In this cross-sectional study during 2012-2013, drug susceptibility testing was performed on Mycobacterium tuberculosis isolated in northeast of Iran using proportional method...
2015: BioMed Research International
https://www.readbyqxmd.com/read/25945329/evaluation-of-the-ribosomal-protein-s1-gene-rpsa-as-a-novel-biomarker-for-mycobacterium-species-identification
#17
Hongfei Duan, Guan Liu, Xiaobo Wang, Yuhong Fu, Qian Liang, Yuanyuan Shang, Naihui Chu, Hairong Huang
OBJECTIVES: To evaluate the resolution and reliability of the rpsA gene, encoding ribosomal protein S1, as a novel biomarker for mycobacteria species identification. METHODS: A segment of the rpsA gene (565 bp) was amplified by PCR from 42 mycobacterial reference strains, 172 nontuberculosis mycobacteria clinical isolates, and 16 M. tuberculosis complex clinical isolates. The PCR products were sequenced and aligned by using the multiple alignment algorithm in the MegAlign package (DNASTAR) and the MEGA program...
2015: BioMed Research International
https://www.readbyqxmd.com/read/25494865/a-case-of-panniculitis-caused-by-mycobacterium-massiliense-mimicking-erythema-induratum
#18
S H Park, J K Chae, E J Kim, K Park
Among nontuberculosis mycobacteria (NTM), rapidly growing mycobacteria (RGM) are the most common causative agents of soft tissue infection. Mycobacterium massiliense, a new species of NTM, was isolated in 2004. Due to the lower virulence of RGM, M. massiliense infection is rare in the general population. Here, we report a case of multiple infective panniculitis, due to M. massiliense, mimicking erythema induratum in a patient with Cushing syndrome. The organism was identified using traditional mycobacterial culturing and staining methods as well as molecular approaches, including erythromycin ribosome transferase gene polymerase chain reaction...
July 2015: British Journal of Dermatology
https://www.readbyqxmd.com/read/25286094/polymerase-chain-reaction-restriction-fragment-length-polymorphism-analysis-for-the-differentiation-of-mycobacterial-species-in-bronchial-washings
#19
D K Weerasekera, D N Magana-Arachchi, D Madegedara, N Dissanayake
OBJECTIVES: To identify mycobacterial species in bronchoscopy specimens with a simple assay based on polymerase chain reaction and restriction enzyme digestion. METHODS: Sputum smear negative, bronchoscopy specimens (n=202) were collected from patients attending the Central Chest Clinic and the Teaching Hospital Kandy, Sri Lanka. DNA, extracted from the mycobacterial cultures (n=43) were amplified using known mycobacterial specific Sp1 and Sp2 primers. Resulting products were digested with HaeIII and CfoI restriction enzymes and DNA sequencing was performed for the selected isolates...
September 2014: Ceylon Medical Journal
https://www.readbyqxmd.com/read/25013147/draft-genome-sequence-of-mycobacterium-neoaurum-strain-dsm-44074t
#20
Michael Phelippeau, Catherine Robert, Olivier Croce, Didier Raoult, Michel Drancourt
We report the draft genome sequence of Mycobacterium neoaurum strain DSM 44074(T), a nontuberculosis species responsible for opportunistic infections in immunocompromised patients. The strain described here is composed of 5,536,033 bp, with a G+C content of 66.24%, and carries 5,274 protein-coding genes and 72 RNA genes.
2014: Genome Announcements
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