keyword
MENU ▼
Read by QxMD icon Read
search

Brain Slice

keyword
https://www.readbyqxmd.com/read/28449351/long-term-plasticity-of-corticostriatal-synapses-is-modulated-by-pathway-specific-co-release-of-opioids-through-kappa-opioid-receptors
#1
Sarah L Hawes, Armando G Salinas, David M Lovinger, Kim T Blackwell
Synaptic plasticity in striatum adjusts behaviour adaptively during skill learning, or maladaptively in the case of addiction. Just as dopamine plays a critical role in synaptic plasticity underlying normal skill learning and addiction, endogenous and exogenous opiates also modulate learning and addiction-related striatal plasticity. Though the role of opioid receptors in long-term depression in striatum has been characterized, their effect on long-term potentiation (LTP) remains unknown. In particular, direct pathway (Dopamine D1 Receptor containing; D1R) - spiny projection neurons (SPNs) co-release the opioid neuropeptide dynorphin, which acts at presynaptic kappa opioid receptors (KORs) on dopaminergic afferents and can negatively regulate dopamine release...
April 27, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28449208/complex-modulation-by-stress-of-the-effect-of-seizures-on-long-term-potentiation-in-mouse-hippocampal-slices
#2
Nicola Maggio, Efrat Shavit Stein, Menahem Segal
Stress has a profound effect on ability to express neuronal plasticity, learning and memory. Likewise, epileptic seizures lead to massive changes in brain connectivity, and in ability to undergo long term changes in reactivity to afferent stimulation. In the present study we analyzed possible long lasting interactions between a stressful experience and reactivity to pilocarpine, on the ability to produce long term potentiation (LTP) in a mouse hippocampus. Pilocarpine lowers paired pulse potentiation (PPP) as well as LTP in CA1 region of the mouse hippocampal slice...
April 27, 2017: Hippocampus
https://www.readbyqxmd.com/read/28448422/iterative-reconstruction-designed-for-brain-ct-a-correlative-study-with-filtered-back-projection-for-the-diagnosis-of-acute-ischemic-stroke
#3
Yuji Iyama, Takeshi Nakaura, Seitaro Oda, Masafumi Kidoh, Daisuke Utsunomiya, Morikatsu Yoshida, Hideaki Yuki, Kenichiro Hirata, Yoshinori Funama, Kazunori Harada, Kazuo Awai, Toshinori Hirai, Yasuyuki Yamashita
OBJECTIVES: The objective of this study is to evaluate the usefulness of iterative model reconstruction designed for brain computed tomography (CT) (IMR-Neuro) for the diagnosis of acute ischemic stroke. METHODS: This retrospective study included 20 patients with acute middle cerebral artery infarction who have undergone brain CT and 20 nonstroke patients (control). We reconstructed axial images with filtered back projection (FBP) and IMR-Neuro (slice thickness, 1 and 5 mm)...
April 26, 2017: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/28443061/a-linear-temporal-increase-in-thrombin-activity-and-loss-of-its-receptor-in-mouse-brain-following-ischemic-stroke
#4
Doron Bushi, Efrat Shavit Stein, Valery Golderman, Ekaterina Feingold, Orna Gera, Joab Chapman, David Tanne
BACKGROUND: Brain thrombin activity is increased following acute ischemic stroke and may play a pathogenic role through the protease-activated receptor 1 (PAR1). In order to better assess these factors, we obtained a novel detailed temporal and spatial profile of thrombin activity in a mouse model of permanent middle cerebral artery occlusion (pMCAo). METHODS: Thrombin activity was measured by fluorescence spectroscopy on coronal slices taken from the ipsilateral and contralateral hemispheres 2, 5, and 24 h following pMCAo (n = 5, 6, 5 mice, respectively)...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28435102/simultaneous-time-interleaved-multislice-stims-for-rapid-susceptibility-weighted-acquisition
#5
Berkin Bilgic, Huihui Ye, Lawrence L Wald, Kawin Setsompop
T2⁎ weighted 3D Gradient Echo (GRE) acquisition is the main sequence used for Susceptibility Weighted Imaging (SWI) and Quantitative Susceptibility Mapping (QSM). These applications require a long echo time (TE) to build up phase contrast, requiring a long repetition time (TR), and leading to excessively lengthy scans. The long TE acquisition creates a significant amount of unused time within each TR, which can be utilized for either multi-echo sampling or additional image encoding with the echo-shift technique...
April 20, 2017: NeuroImage
https://www.readbyqxmd.com/read/28435053/in-depth-analysis-of-the-synaptic-plasma-membrane-proteome-of-small-hippocampal-slices-using-an-integrated-approach
#6
Rui Qiao, Shuiming Li, Mi Zhou, Penghui Chen, Zhao Liu, Min Tang, Jian Zhou
Comprehensive knowledge of the synaptic plasma membrane (SPM) proteome of a distinct brain region in a defined pathological state would greatly advance the understanding of the underlying biology of synaptic plasticity. The development of innovative approaches for studying the SPM proteome of small brain tissues is highly desired. This study presents a suitable protocol that integrates biotinylation-based affinity capture of cell surface-exposed proteins, isolation of synaptosomes, and biochemical extraction of SPM proteins from biotinylated hippocampal slices...
April 20, 2017: Neuroscience
https://www.readbyqxmd.com/read/28434890/brain-lipidomic-changes-after-morphine-cocaine-and-amphetamine-administration-desi-ms-imaging-study
#7
Anna Bodzon-Kulakowska, Anna Antolak, Anna Drabik, Marta Marszalek-Grabska, Jolanta Kotlińska, Piotr Suder
Drug addiction is a complex disorder, evoking significant changes in the proteome of the central nervous system. To check if there are also changes in the lipidomic profiles we used desorption electrospray-MS technique for imaging of the brain slices of rats exposed to morphine, cocaine and amphetamine. Our investigations showed alternative regulation of selected lipid's levels in the central nervous system structures, under the influence of applied drugs. Results of our investigations can show changes in the brain treated with drugs of abuse in the new light, indicating role of the lipids in the addiction development...
April 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28433624/temporal-slice-registration-and-robust-diffusion-tensor-reconstruction-for-improved-fetal-brain-structural-connectivity-analysis
#8
Bahram Marami, Seyed Sadegh Mohseni Salehi, Onur Afacan, Benoit Scherrer, Caitlin K Rollins, Edward Yang, Judy A Estroff, Simon K Warfield, Ali Gholipour
Diffusion weighted magnetic resonance imaging, or DWI, is one of the most promising tools for the analysis of neural microstructure and the structural connectome of the human brain. The application of DWI to map early development of the human connectome in-utero, however, is challenged by intermittent fetal and maternal motion that disrupts the spatial correspondence of data acquired in the relatively long DWI acquisitions. Fetuses move continuously during DWI scans. Reliable and accurate analysis of the fetal brain structural connectome requires careful compensation of motion effects and robust reconstruction to avoid introducing bias based on the degree of fetal motion...
April 19, 2017: NeuroImage
https://www.readbyqxmd.com/read/28431630/kynurenic-acid-and-its-analogue-can-alter-the-opioid-receptor-g-protein-signaling-after-acute-treatment-via-nmda-receptor-in-rat-cortex-and-striatum
#9
Reza Samavati, Ferenc Zádor, Edina Szűcs, Bernadett Tuka, Diána Martos, Gábor Veres, Róbert Gáspár, István M Mándity, Ferenc Fülöp, László Vécsei, Sándor Benyhe, Anna Borsodi
Previously, we have shown that the N-methyl d-aspartate (NMDA)-receptor antagonist kynurenic acid (KYNA) and its analogue KYNA1 do not bind directly to mu, kappa and delta opioid receptors in vitro. On the other hand, chronic administration of KYNA and KYNA1 resulted in region (cortex vs striatum) and opioid receptor-type specific alterations in G-protein activation of mouse brain homogenates. Here we describe for the first time the acute effect of KYNA and KYNA1 on opioid receptor function with the possible involvement of the NMDA receptor...
May 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28430864/the-gnih-lpxrfa-system-s-regulation-of-reproduction-in-the-brain-pituitary-axis-of-the-zebrafish-danio-rerio
#10
Olivia Smith Spicer, Nilli Zmora, Ten-Tsao Wong, Matan Golan, Berta Levavi-Sivan, Yoav Gothilf, Yonathan Zohar
Gonadotropin-inhibitory hormone (GNIH) was discovered in quail with the ability to reduce gonadotropin expression/secretion in the pituitary. There have been few studies on GNIH orthologs in teleosts (Lpxrfa peptides), which have provided inconsistent results. Therefore, the goal of this study was to determine the roles and modes of action by which Lpxrfa exerts its functions in the brain-pituitary axis of zebrafish (Danio rerio). We localized Lpxrfa soma to the ventral hypothalamus, with fibers extending throughout the brain and to the pituitary...
April 18, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28429885/intravenous-self-administration-of-benzydamine-a-non-steroidal-anti-inflammatory-drug-with-a-central-cannabinoidergic-mechanism-of-action
#11
Riccardo Avvisati, Maria Meringolo, Emiliana Stendardo, Elisa Malavasi, Silvia Marinelli, Aldo Badiani
Benzydamine (BZY) is a non-steroidal anti-inflammatory drug used for the topical treatment of inflammations of the oral and vaginal mucosae. Virtually nothing is known about the central pharmacological actions of BZY. Yet there are reports of voluntary systemic overdosage of BZY in drug addicts, resulting in a euphoric, hallucinatory state. In the present study, we investigated the reinforcing properties of BZY in a rat self-administration paradigm. We found that BZY has a powerful reinforcing effect and that this effect is greatly facilitated in animals that already had substance experience, having previously self-administered heroin and cocaine, indicating cross sensitization between BZY and other common drugs of abuse...
April 21, 2017: Addiction Biology
https://www.readbyqxmd.com/read/28428209/myelin-detection-using-rapid-quantitative-mr-imaging-correlated-to-macroscopically-registered-luxol-fast-blue-stained-brain-specimens
#12
J B M Warntjes, A Persson, J Berge, W Zech
BACKGROUND AND PURPOSE: Myelin detection is of great value in monitoring diseases such as multiple sclerosis and dementia. However, most MR imaging methods to measure myelin are challenging for routine clinical use. Recently, a novel method was published, in which the presence of myelin is inferred by using its effect on the intra- and extracellular water relaxation rates and proton density, observable by rapid quantitative MR imaging. The purpose of this work was to validate this method further on the brains of 12 fresh, intact cadavers...
April 20, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28426965/formation-and-maintenance-of-functional-spines-in-the-absence-of-presynaptic-glutamate-release
#13
Albrecht Sigler, Won Chan Oh, Cordelia Imig, Bekir Altas, Hiroshi Kawabe, Benjamin H Cooper, Hyung-Bae Kwon, Jeong-Seop Rhee, Nils Brose
Dendritic spines are the major transmitter reception compartments of glutamatergic synapses in most principal neurons of the mammalian brain and play a key role in the function of nerve cell circuits. The formation of functional spine synapses is thought to be critically dependent on presynaptic glutamatergic signaling. By analyzing CA1 pyramidal neurons in mutant hippocampal slice cultures that are essentially devoid of presynaptic transmitter release, we demonstrate that the formation and maintenance of dendrites and functional spines are independent of synaptic glutamate release...
April 19, 2017: Neuron
https://www.readbyqxmd.com/read/28426226/brain-uptake-of-bioactive-flavones-in-scutellariae-radix-and-its-relationship-to-anxiolytic-effect-in-mice
#14
Sophia Yui Kau Fong, Chenrui Li, Yiu Cheong Ho, Rui Li, Qian Wang, Yin Cheong Wong, Hong Xue, Zhong Zuo
Scutellariae Radix (SR) and its bioactive flavones elicit a variety of effects in the brain. However, the brain uptake of individual SR flavones and its relationship to the elicited effects after SR administration remain unknown. Moreover, previous studies seldom measured pharmacokinetic and pharmacodynamic outcomes simultaneously. In the current study, the brain uptake of six major SR flavones and the anxiolytic behavior following oral administration of a SR extract at two clinically relevant doses (600 and 1200 mg/kg twice daily) were simultaneously investigated in mice (n=18 per group)...
April 20, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28424572/functional-sensitivity-of-2d-simultaneous-multi-slice-echo-planar-imaging-effects-of-acceleration-on-g-factor-and-physiological-noise
#15
Nick Todd, Oliver Josephs, Peter Zeidman, Guillaume Flandin, Steen Moeller, Nikolaus Weiskopf
Accelerated data acquisition with simultaneous multi-slice (SMS) imaging for functional MRI studies leads to interacting and opposing effects that influence the sensitivity to blood oxygen level-dependent (BOLD) signal changes. Image signal to noise ratio (SNR) is decreased with higher SMS acceleration factors and shorter repetition times (TR) due to g-factor noise penalties and saturation of longitudinal magnetization. However, the lower image SNR is counteracted by greater statistical power from more samples per unit time and a higher temporal Nyquist frequency that allows for better removal of spurious non-BOLD high frequency signal content...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28424512/human-umbilical-cord-plasma-proteins-revitalize-hippocampal-function-in-aged-mice
#16
Joseph M Castellano, Kira I Mosher, Rachelle J Abbey, Alisha A McBride, Michelle L James, Daniela Berdnik, Jadon C Shen, Bende Zou, Xinmin S Xie, Martha Tingle, Izumi V Hinkson, Martin S Angst, Tony Wyss-Coray
Ageing drives changes in neuronal and cognitive function, the decline of which is a major feature of many neurological disorders. The hippocampus, a brain region subserving roles of spatial and episodic memory and learning, is sensitive to the detrimental effects of ageing at morphological and molecular levels. With advancing age, synapses in various hippocampal subfields exhibit impaired long-term potentiation, an electrophysiological correlate of learning and memory. At the molecular level, immediate early genes are among the synaptic plasticity genes that are both induced by long-term potentiation and downregulated in the aged brain...
April 19, 2017: Nature
https://www.readbyqxmd.com/read/28424399/-recent-advancements-in-understanding-hippocampal-functions
#17
Sakura Okada, Yuki Aoki, Takuya Sasaki, Yuji Ikegaya
The hippocampal formation located in the medial temporal lobe of the brain has been studied extensively for several decades, both in in vitro slice preparations and in vivo living animals. Recent advancements in large-scale recording techniques, such as multisite silicon probes and tetrodes, enable us to examine the detailed characteristics of temporal spiking patterns of tens or hundreds of neurons in the hippocampal circuit. Here, we introduce how such tools contribute to our understanding of the hippocampal network dynamics and review several hippocampal functions for information processing in the brain, including pattern separation and completion, spatial representation, and encoding of positive and negative valence...
April 2017: Brain and Nerve, Shinkei Kenkyū No Shinpo
https://www.readbyqxmd.com/read/28424297/magnitude-and-behavior-of-cross-talk-effects-in-multi-channel-electrophysiology-experiments
#18
Matthew John Nelson, Silvana Valtcheva, Laurent Venance
Modern neurophysiological experiments frequently involve multiple channels separated by very small distances. A unique methodological concern for multiple electrode experiments is that of capacitive coupling (cross-talk) between channels. Yet the nature of the cross-talk recording circuit is not well known in the field and the extent to which it practically affects neurophysiology experiments has never been fully investigated. Here we describe a simple electrical circuit model of simultaneous recording and stimulation with two or more channels and experimentally verify the model using ex vivo brain slice and in vivo whole-brain preparations...
April 19, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28421851/global-and-multi-focal-changes-in-cerebral-blood-flow-during-subthalamic-nucleus-stimulation-in-parkinson-s-disease
#19
Asim M Mubeen, Babak Ardekani, Michele Tagliati, Ron Alterman, Vijay Dhawan, David Eidelberg, John J Sidtis
Electrical stimulation of subthalamic nuclei (STN) is a widely used therapy in Parkinson's disease (PD). While deep brain stimulation (DBS) of the STN alters the neurophysiological activity in basal ganglia, the therapeutic mechanism has not been established. A positron emission tomography (PET) study of cerebral blood flow (CBF) during speech production in PD subjects treated with STN-DBS found significant increases in global (whole-brain) CBF.(1) That study utilized a series of whole-slice regions of interest to obtain global CBF values...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28419506/neuronal-loss-demyelination-and-volume-change-in-the-multiple-sclerosis-neocortex
#20
D Carassiti, D R Altmann, N Petrova, B Pakkenberg, F Scaravilli, K Schmierer
AIMS: Indices of brain volume (grey matter, white matter, lesions) are being used as outcomes in clinical trials of patients with multiple sclerosis (MS). We investigated the relationship between cortical volume, the number of neocortical neurons estimated using stereology, and demyelination. METHODS: Nine MS and seven control hemispheres were dissected into coronal slices. On sections stained for Giemsa, the cortex was outlined and optical disectors applied using systematic uniform random sampling...
April 18, 2017: Neuropathology and Applied Neurobiology
keyword
keyword
40401
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"