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https://www.readbyqxmd.com/read/29054120/cover-image-volume-118-number-12-december-2017
#1
Duo Wang, Chang Liu, Zhigang Li, Yumei Wang, Wenjing Wang, Xiujuan Wu, Kang Wang, Wei Miao, Li Li, Luying Peng
Cover: The cover image, by Duo Wang et al., is based on the Article Regulation of Histone Acetylation on Expression Profiles of Potassium Channels During Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells, DOI: 10.1002/jcb.26102.
December 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29054108/increase-in-pro-apoptotic-activity-of-inhibitory-pas-domain-protein-via-phosphorylation-by-mk2
#2
Shuya Kasai, Mary Jane Elizabeth Richardson, Satoru Torii, Ken-Ichi Yasumoto, Hiroki Shima, Kazuhiko Igarashi, Ken Itoh, Kazuhiro Sogawa, Kazutaka Murayama
Inhibitory PAS domain protein (IPAS) is a bifunctional protein that downregulates hypoxic gene expression and exerts pro-apoptotic activity by preventing pro-survival activity of Bcl-xL and its related factors. Pro-apoptotic activity of IPAS is attenuated by the activation of the PINK1-Parkin pathway, and involved in neuronal degeneration in an experimental mouse model of Parkinson's disease. The current study shows that phosphorylation of IPAS at Ser184 by MAPK-activated protein kinase 2 (MK2 or MAPKAPK2) enhances the pro-apoptotic function of IPAS...
October 20, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29054094/runx3-inhibits-the-proliferation-and-metastasis-of-gastric-cancer-through-regulating-mir-182-hoxa9
#3
Junyan Yu, Xiangyang Tian, Jianlan Chang, Ping Liu, Rong Zhang
OBJECTIVE: This study intended to explore the molecular mechanism of RUNX3 in inhibiting the process of migration and proliferation of gastric cancer (GC) cells. METHODS: The overexpressed plasmids of RUNX3 and the interfering siRNA of RUNX3 were transfected into GC cells. Then, qRT-PCR and western blot were performed to identify the expression level of RUNX3 and miR-182 in tumors and adjacent tissues respectively. ChIP and luciferase assay were performed to detect the relationship between RUNX3 and miR-182 as well as miR-182 and HOXA9...
October 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29054053/leptin-reduces-microrna-122-level-in-hepatic-stellate-cells-in-vitro-and-in-vivo
#4
Xuguang Zhai, Fangyun Cheng, Li Ji, Xiaofei Zhu, Qing Cao, Yali Zhang, Xin Jia, Qian Zhou, Wei Guan, Yajun Zhou
Obese patients, often accompanied by hyperleptinemia, are more likely to develop liver fibrosis. Leptin, an adipocyte-derived hormone, augments inflammatory in liver and promotes hepatic stellate cell (HSC) activation (a key step for liver fibrogenesis) and liver fibrosis. microRNA-122 (miR-122) is the most abundant liver-specific miRNA and can attenuate liver fibrosis. This study examined the effect of leptin on miR-122 level in HSCs in vivo and in vitro. Results demonstrated that leptin reduced the levels of both miR-122 (mature miR-122) and primary miR-122 (pri-miR-122)...
October 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29053994/dietary-resveratrol-confers-apoptotic-resistance-to-oxidative-stress-in-myoblasts
#5
Satoshi Haramizu, Shinichi Asano, David C Butler, David A Stanton, Ameena Hajira, Junaith S Mohamed, Stephen E Alway
High levels of reactive oxygen species (ROS) contribute to muscle cell death in aging and disuse. We have previously found that resveratrol can reduce oxidative stress in response to aging and hindlimb unloading in rodents in vivo, but it was not known if resveratrol would protect muscle stem cells during repair or regeneration when oxidative stress is high. To test the protective role of resveratrol on muscle stem cells directly, we treated the C2C12 mouse myoblast cell line with moderate (100 μM) or very high (1 mM) levels of H2O2 in the presence or absence of resveratrol...
August 24, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29053991/molecular-mechanisms-of-development-of-the-human-fetal-female-reproductive-tract
#6
Gerald R Cunha, Takeshi Kurita, Mei Cao, Joel Shen, Stanley Robboy, Laurence Baskin
Human female reproductive tract development rests mostly upon hematoxilyn and eosin stained sections despite recent advances on molecular mechanisms in mouse studies. We report application of immunohistochemical methods to explore the ontogeny of epithelial and mesenchymal differentiation markers (keratins, homobox proteins, steroid receptors), transcription factors and signaling molecules (TP63 and RUNX1) during human female reproductive tract development. Keratins 6, 7, 8, 10, 14 and 19 (KRT6, KRT7, KRT8, KRT10, KRT14, KRT19) were expressed in a temporally and spatially dynamic fashion...
July 29, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29053988/intermedin-reduces-neointima-formation-by-regulating-vascular-smooth-muscle-cell-phenotype-via-camp-pka-pathway
#7
Qing Zhu, Xian-Qiang Ni, Wei-Wei Lu, Jin-Sheng Zhang, Jin-Ling Ren, Di Wu, Yao Chen, Lin-Shuang Zhang, Yan-Rong Yu, Chao-Shu Tang, Yong-Fen Qi
BACKGROUND AND AIMS: Vascular smooth muscle cell (VSMC) dedifferentiation contributes to neointima formation, which results in various vascular disorders. Intermedin (IMD), a cardiovascular paracrine/autocrine polypeptide, is involved in maintaining circulatory homeostasis. However, whether IMD protects against neointima formation remains largely unknown. The purpose of this study is to investigate the role of IMD in neointima formation and the possible mechanism. METHODS: IMD1-53 (100ng/kg/h) or saline water was used on rat carotid-artery balloon-injury model...
October 9, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29053968/multiscale-3d-genome-rewiring-during-mouse-neural-development
#8
Boyan Bonev, Netta Mendelson Cohen, Quentin Szabo, Lauriane Fritsch, Giorgio L Papadopoulos, Yaniv Lubling, Xiaole Xu, Xiaodan Lv, Jean-Philippe Hugnot, Amos Tanay, Giacomo Cavalli
Chromosome conformation capture technologies have revealed important insights into genome folding. Yet, how spatial genome architecture is related to gene expression and cell fate remains unclear. We comprehensively mapped 3D chromatin organization during mouse neural differentiation in vitro and in vivo, generating the highest-resolution Hi-C maps available to date. We found that transcription is correlated with chromatin insulation and long-range interactions, but dCas9-mediated activation is insufficient for creating TAD boundaries de novo...
October 19, 2017: Cell
https://www.readbyqxmd.com/read/29053957/cdk4-phosphorylates-ampk%C3%AE-2-to-inhibit-its-activity-and-repress-fatty-acid-oxidation
#9
Isabel C Lopez-Mejia, Sylviane Lagarrigue, Albert Giralt, Laia Martinez-Carreres, Nadège Zanou, Pierre-Damien Denechaud, Judit Castillo-Armengol, Carine Chavey, Meritxell Orpinell, Brigitte Delacuisine, Anita Nasrallah, Caterina Collodet, Lianjun Zhang, Benoît Viollet, D Grahame Hardie, Lluis Fajas
The roles of CDK4 in the cell cycle have been extensively studied, but less is known about the mechanisms underlying the metabolic regulation by CDK4. Here, we report that CDK4 promotes anaerobic glycolysis and represses fatty acid oxidation in mouse embryonic fibroblasts (MEFs) by targeting the AMP-activated protein kinase (AMPK). We also show that fatty acid oxidation (FAO) is specifically induced by AMPK complexes containing the α2 subunit. Moreover, we report that CDK4 represses FAO through direct phosphorylation and inhibition of AMPKα2...
October 19, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29053948/nanoscale-imaging-reveals-big-role-for-iron-in-alzheimer-s-disease
#10
Scott Ayton, Simon A James, Ashley I Bush
In this issue of Cell Chemical Biology, Telling et al. (2017) apply advanced X-ray microscopy techniques to reveal magnetite iron species in plaques from a mouse model of Alzheimer's disease. The characterization of abnormal iron chemistry in the disease model highlights the potential for iron to combine with the β-amyloid peptide and accelerate the disease process.
October 19, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/29053899/peptidergic-nerve-fibers-in-the-urethra-morphological-and-neurochemical-characteristics-in-female-mice-of-reproductive-age
#11
Christine M Barry, Esther Ji, Harman Sharma, Pauline Yap, Nicholas J Spencer, Dusan Matusica, Rainer V Haberberger
BACKGROUND: Peptidergic nerve fibers provide important contributions to urethral function. Urethral innervation of female mice is not well documented. AIMS: To determine the distribution and projection sites of nerve fibers immunoreactive for vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP), and neuropeptide Y (NPY) in the urethra of wild-type control mice and compare innervation characteristics between the proximal and distal urethra of young nullipara and older multipara mice...
October 20, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29053877/trpv4-regulates-vascular-endothelial-permeability-during-colonic-inflammation-in-dextran-sulphate-sodium-induced-murine-colitis
#12
Kenjiro Matsumoto, Riho Yamaba, Ken Inoue, Daichi Utsumi, Takuya Tsukahara, Kikuko Amagase, Makoto Tominaga, Shinichi Kato
BACKGROUND AND PURPOSE: The transient receptor potential vanilloid 4 (TRPV4) is a nonselective cation channel involved in physical sensing in various tissue types. The present study aimed to elucidate the function and expression of TRPV4 in colonic vascular endothelial cells during dextran sulphate sodium (DSS)-induced colitis. EXPERIMENTAL APPROACH: The role of TRPV4 in the progression of colonic inflammation was examined in the 2 % DSS-induced murine colitis model using immunohistochemical analysis, western blotting, and Evans blue dye extrusion assay...
October 20, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29053824/chemogenetic-locus-coeruleus-activation-restores-reversal-learning-in-a-rat-model-of-alzheimer-s-disease
#13
Jacki M Rorabaugh, Termpanit Chalermpalanupap, Christian A Botz-Zapp, Vanessa M Fu, Natalie A Lembeck, Robert M Cohen, David Weinshenker
Clinical evidence suggests that aberrant tau accumulation in the locus coeruleus and noradrenergic dysfunction may be a critical early step in Alzheimer's disease progression. Yet, an accurate preclinical model of these phenotypes that includes early pretangle tau accrual in the locus coeruleus, loss of locus coeruleus innervation and deficits locus coeruleus/norepinephrine modulated behaviours, does not exist, hampering the identification of underlying mechanisms and the development of locus coeruleus-based therapies...
September 25, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29053787/humanized-mutant-fus-drives-progressive-motor-neuron-degeneration-without-aggregation-in-fusdelta14-knockin-mice
#14
Anny Devoy, Bernadett Kalmar, Michelle Stewart, Heesoon Park, Beverley Burke, Suzanna J Noy, Yushi Redhead, Jack Humphrey, Kitty Lo, Julian Jaeger, Alan Mejia Maza, Prasanth Sivakumar, Cinzia Bertolin, Gianni Soraru, Vincent Plagnol, Linda Greensmith, Abraham Acevedo Arozena, Adrian M Isaacs, Benjamin Davies, Pietro Fratta, Elizabeth M C Fisher
Mutations in FUS are causative for amyotrophic lateral sclerosis with a dominant mode of inheritance. In trying to model FUS-amyotrophic lateral sclerosis (ALS) in mouse it is clear that FUS is dosage-sensitive and effects arise from overexpression per se in transgenic strains. Novel models are required that maintain physiological levels of FUS expression and that recapitulate the human disease-with progressive loss of motor neurons in heterozygous animals. Here, we describe a new humanized FUS-ALS mouse with a frameshift mutation, which fulfils both criteria: the FUS Delta14 mouse...
October 7, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29053761/the-transcription-factor-prdm16-marks-a-single-retinal-ganglion-cell-subtype-in-the-mouse-retina
#15
Sergio Groman-Lupa, Joseph Adewumi, Ko Uoon Park, Joseph A Brzezinski Iv
Purpose: Retinal ganglion cells (RGC) can be categorized into roughly 30 distinct subtypes. How these subtypes develop is poorly understood, in part because few unique subtype markers have been characterized. We tested whether the Prdm16 transcription factor is expressed by RGCs as a class or within particular ganglion cell subtypes. Methods: Embryonic and mature retinal sections and flatmount preparations were examined by immunohistochemistry for Prdm16 and several other cell type-specific markers...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29053753/3d-morphological-analysis-of-the-mouse-cerebral-vasculature-comparison-of-in-vivo-and-ex-vivo-methods
#16
Joe Steinman, Margaret M Koletar, Bojana Stefanovic, John G Sled
Ex vivo 2-photon fluorescence microscopy (2PFM) with optical clearing enables vascular imaging deep into tissue. However, optical clearing may also produce spherical aberrations if the objective lens is not index-matched to the clearing material, while the perfusion, clearing, and fixation procedure may alter vascular morphology. We compared in vivo and ex vivo 2PFM in mice, focusing on apparent differences in microvascular signal and morphology. Following in vivo imaging, the mice (four total) were perfused with a fluorescent gel and their brains fructose-cleared...
2017: PloS One
https://www.readbyqxmd.com/read/29053700/come-to-the-light-side-in-vivo-monitoring-of-pseudomonas-aeruginosa-biofilm-infections-in-chronic-wounds-in-a-diabetic-hairless-murine-model
#17
Alessandra M Agostinho Hunt, Jacob A Gibson, Casandra L Larrivee, Sandra O'Reilly, Svetlana Navitskaya, Julia V Busik, Christopher M Waters
The presence of bacteria as structured biofilms in chronic wounds, especially in diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used to understand the underlying interactions between the microorganisms and the host. The models developed to date rely on the use of haired animals and terminal collection of wound tissue for determination of viable bacteria. While significant insight has been gained with these models, this experimental procedure requires a large number of animals and sampling is time consuming...
October 10, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053691/quantitative-whole-mount-immunofluorescence-analysis-of-cardiac-progenitor-populations-in-mouse-embryos
#18
Evan Bardot, Nikos Tzavaras, Deanna L Benson, Nicole C Dubois
The use of ever-advancing imaging techniques has contributed broadly to our increased understanding of embryonic development. Pre-implantation development and organogenesis are two areas of research that have benefitted greatly from these advances, due to the high quality of data that can be obtained directly from imaging pre-implantation embryos or ex vivo organs. While pre-implantation embryos have yielded data with especially high spatial resolution, later stages have been less amenable to three-dimensional reconstruction...
October 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053690/3d-microtissues-for-injectable-regenerative-therapy-and-high-throughput-drug-screening
#19
Yaqian Li, Xiaojun Yan, Wei Liu, Lyu Zhou, Zhifeng You, Yanan Du
To upgrade traditional 2D cell culture to 3D cell culture, we have integrated microfabrication with cryogelation technology to produce macroporous microscale cryogels (microcryogels), which can be loaded with a variety of cell types to form 3D microtissues. Herein, we present the protocol to fabricate versatile 3D microtissues and their applications in regenerative therapy and drug screening. Size and shape-controllable microcryogels can be fabricated on an array chip, which can be harvested off-chip as individual cell-loaded carriers for injectable regenerative therapy or be further assembled on-chip into 3D microtissue arrays for high-throughput drug screening...
October 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29053682/implantation-of-chronic-silicon-probes-and-recording-of-hippocampal-place-cells-in-an-enriched-treadmill-apparatus
#20
Anvar Sariev, Jinho Chung, Dajung Jung, Farnaz Sharif, Ju-Young Lee, Soyoun Kim, Sebastien Royer
An important requisite for understanding brain function is the identification of behavior and cell activity correlates. Silicon probes are advanced electrodes for large-scale electrophysiological recording of neuronal activity, but the procedures for their chronic implantation are still underdeveloped. The activity of hippocampal place cells is known to correlate with an animal's position in the environment, but the underlying mechanisms are still unclear. To investigate place cells, here we describe a set of techniques which range from the fabrication of devices for chronic silicon probe implants to the monitoring of place field activity in a cue-enriched treadmill apparatus...
October 11, 2017: Journal of Visualized Experiments: JoVE
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