keyword
https://read.qxmd.com/read/38257793/characterization-of-a-thermostable-endolysin-of-the-aeribacillus-phage-aerip45-as-a-potential-staphylococcus-biofilm-removing-agent
#21
JOURNAL ARTICLE
Natalia N Golosova, Yana A Khlusevich, Vera V Morozova, Andrey L Matveev, Yulia N Kozlova, Artem Y Tikunov, Elizaveta A Panina, Nina V Tikunova
Multidrug-resistant Gram-positive bacteria, including bacteria from the genus Staphylococcus , are currently a challenge for medicine. Therefore, the development of new antimicrobials is required. Promising candidates for new antistaphylococcal drugs are phage endolysins, including endolysins from thermophilic phages against other Gram-positive bacteria. In this study, the recombinant endolysin LysAP45 from the thermophilic Aeribacillus phage AP45 was obtained and characterized. The recombinant endolysin LysAP45 was produced in Escherichia coli M15 cells...
January 7, 2024: Viruses
https://read.qxmd.com/read/38252794/enhanced-biofilm-formation-by-tetracycline-in-a-staphylococcus-aureus-naturally-lacking-ica-operon-and-atl
#22
JOURNAL ARTICLE
Yimin Zou, Xuejie Li, Yanxiong Mao, Wenjuan Song, Qing Liu
Staphylococcus aureus is a major, widespread pathogen, and its biofilm-forming characteristics make it even more difficult to eliminate by biocides. Tetracycline (TCY) is a major broad-spectrum antibiotic, the residues of which can cause deleterious health impacts, and subinhibitory concentrations of TCY have the potential to increase biofilm formation in S. aureus . In this study, we showed how the biofilm formation of S. aureus 123786 is enhanced in the presence of TCY at specific subinhibitory concentrations...
February 2024: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://read.qxmd.com/read/38248691/surface-properties-of-a-biocompatible-thermoplastic-polyurethane-and-its-anti-adhesive-effect-against-e-coli-and-s-aureus
#23
JOURNAL ARTICLE
Elisa Restivo, Emanuela Peluso, Nora Bloise, Giovanni Lo Bello, Giovanna Bruni, Marialaura Giannaccari, Roberto Raiteri, Lorenzo Fassina, Livia Visai
Thermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no significant differences between brush and bar coater-modified surfaces, compared to the un-modified TPU (TPU Film). The effect of the surfaces on planktonic bacteria, evaluated by MTT assay, demonstrated their anti-adhesive effect on E...
January 15, 2024: Journal of Functional Biomaterials
https://read.qxmd.com/read/38245727/combination-antimicrobial-therapy-in-vitro-synergistic-effect-of-anti-staphylococcal-drug-oxacillin-with-antimicrobial-peptide-nisin-against-staphylococcus-epidermidis-clinical-isolates-and-staphylococcus-aureus-biofilms
#24
JOURNAL ARTICLE
Toktam Sharafi, Ezzat Allah Ghaemi, Maryam Rafiee, Abdollah Ardebili
The ability of Staphylococcus epidermidis and S. aureus to form strong biofilm on plastic devices makes them the major pathogens associated with device-related infections (DRIs). Biofilm-embedded bacteria are more resistant to antibiotics, making biofilm infections very difficult to effectively treat. Here, we evaluate the in vitro activities of anti-staphylococcal drug oxacillin and antimicrobial peptide nisin, alone and in combination, against methicillin-resistant S. epidermidis (MRSE) clinical isolates and the methicillin-resistant S...
January 20, 2024: Annals of Clinical Microbiology and Antimicrobials
https://read.qxmd.com/read/38245549/non-biofilm-forming-staphylococcus-epidermidis-planktonic-cell-supernatant-induces-alterations-in-osteoblast-biological-function
#25
JOURNAL ARTICLE
Itzia Sidney Gómez-Alonso, Gabriel Betanzos-Cabrera, Martha Cecilia Moreno-Lafont, Mario Eugenio Cancino-Diaz, Blanca Estela García-Pérez, Juan Carlos Cancino-Diaz
Staphylococcal biofilms significantly contribute to prosthetic joint infection (PJI). However, 40% of S. epidermidis PJI isolates do not produce biofilms, which does not explain the role of biofilms in these cases. We studied whether the supernatant from planktonic S. epidermidis alters osteoblast function. Non-biofilm-forming S. epidermidis supernatants (PJI- clinical isolate, healthy skin isolate (HS), and ATCC12228 reference strain) and biofilm-forming supernatants (PJI+ clinical isolate, ATCC35984 reference strain, and Staphylococcus aureus USA300 reference strain) were included...
January 20, 2024: Scientific Reports
https://read.qxmd.com/read/38202618/-pinus-koraiensis-essential-oil-attenuates-the-pathogenicity-of-superbacteria-by-suppressing-virulence-gene-expression
#26
JOURNAL ARTICLE
Ji-Hee Kim, Young-Hoi Kim, Bog-Im Park, Na-Young Choi, Kang-Ju Kim
In the quest to combat infections attributable to antibiotic-resistant superbacteria, an essential oil derived from the needles of Pinus koraiensis Sieb. et Zucc. (PKEO) has emerged as a promising solution. In this study, we demonstrate that PKEO can be used to inhibit the growth, glucose metabolite acidogenicity, and biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA). Quantitative PCR analysis provided direct evidence that PKEO reduces the mRNA expression of the accessory gene regulator A ( agrA ) and staphylococcal accessory regulator A ( sarA ), thereby indicating its inhibitory effect on pathogenic regulatory genes...
December 20, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38170998/structure-guided-design-synthesis-and-antivirulence-assessment-of-covalent-staphylococcus-aureus-sortase-a-inhibitors
#27
JOURNAL ARTICLE
Chuan Yue, Ziqi Yuan, Guobin Xu, Xiang-Na Guan, Bingyan Wei, Hequan Yao, Cai-Guang Yang, Tao Zhang
Sortase A (SrtA) is a membrane-associated cysteine transpeptidase required for bacterial virulence regulation and anchors surface proteins to cell wall, thereby assisting biofilm formation. SrtA is targeted in antivirulence treatments against Gram-positive bacterial infections. However, the development of potent small-molecule SrtA inhibitors is constrained owing to the limited understanding of the mode of action of inhibitors in the SrtA binding pocket. Herein, we designed and synthesized a novel class of covalent SrtA inhibitors based on the binding mode detailed in the X-ray crystal structure of the ML346 / Streptococcus pyogenes SrtA complex...
January 3, 2024: Journal of Medicinal Chemistry
https://read.qxmd.com/read/38160879/biofilms-a-developmental-niche-for-vancomycin-intermediate-staphylococcus-aureus
#28
JOURNAL ARTICLE
Jenelle E Chapman, Shilpa E George, Christiane Wolz, Michael E Olson
Staphylococcus aureus are gram-positive bacteria responsible for a wide array of diseases, ranging from skin and soft tissue infections to more chronic illnesses such as toxic shock syndrome, osteomyelitis, and endocarditis. Vancomycin is currently one of the most effective antibiotics available in treating patients infected with methicillin-resistant S. aureus (MRSA), however the emergence of vancomycin-resistant S. aureus (VRSA), and more commonly vancomycin-intermediate S. aureus (VISA), threaten the future efficacy of vancomycin...
January 2024: Infection, Genetics and Evolution
https://read.qxmd.com/read/38147401/investigating-the-bispecific-lead-compounds-against-methicillin-resistant-staphylococcus-aureus-sara-and-crtm-using-machine-learning-and-molecular-dynamics-approach
#29
JOURNAL ARTICLE
Kareem M Younes, Amr S Abouzied, Ahmed Alafnan, Bader Huwaimel, Weam M A Khojali, Rami M Alzahrani
Methicillin-resistant Staphylococcus aureus (MRSA) is a notorious pathogen that has emerged as a serious global health concern over the past few decades. Staphylococcal accessory regulator A (SarA) and 4,4'-diapophytoene synthase (CrtM) play a crucial role in biofilm formation and staphyloxanthin biosynthesis. Thus, the present study used a machine learning-based QSAR model to screen 1261 plant-derived natural organic compounds in order to identify a medication candidate with both biofilm and virulence inhibitory potential...
December 26, 2023: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/38140529/isolation-and-characterization-of-new-bacteriophages-against-staphylococcal-clinical-isolates-from-diabetic-foot-ulcers
#30
JOURNAL ARTICLE
Lucile Plumet, Madjid Morsli, Nour Ahmad-Mansour, Fernando Clavijo-Coppens, Laurence Berry, Albert Sotto, Jean-Philippe Lavigne, Denis Costechareyre, Virginie Molle
Staphylococcus sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems. Phage therapy is an alternative treatment to antibiotics, overcoming the issue of antibiotic resistance. In this study, six phages (SAVM01 to SAVM06) were isolated from effluents and were used against a panel of staphylococcal clinical samples isolated from DFUs. A genomic analysis revealed that the phages belonged to the Herelleviridae family, with sequences similar to those of the Kayvirus genus...
November 22, 2023: Viruses
https://read.qxmd.com/read/38136737/longitudinal-study-of-antibiotic-resistance-of-staphylococci-from-cases-of-subclinical-mastitis-in-sheep-in-greece-incidence-and-risk-factors
#31
JOURNAL ARTICLE
Charalambia K Michael, Daphne T Lianou, Katerina Tsilipounidaki, Zoe Florou, Natalia G C Vasileiou, Vasia S Mavrogianni, Efthymia Petinaki, George C Fthenakis
The present paper extends a previous publication on a field study of subclinical mastitis in sheep and focuses on the following laboratory characteristics of the staphylococcal isolates: antibiotic resistance and association with biofilm formation. The specific objectives of the present study were (a) to describe the incidence of isolation of antibiotic-resistant staphylococci from cases of mastitis throughout the milking period in dairy sheep flocks and (b) to identify relevant risk factors, which would contribute to the sustainable control of the infection...
December 7, 2023: Antibiotics
https://read.qxmd.com/read/38062361/staphylococcal-superantigen-like-protein-10-enhances-the-amyloidogenic-biofilm-formation-in-staphylococcus-aureus
#32
JOURNAL ARTICLE
Shakilur Rahman, Amit Kumar Das
Staphylococcus aureus is a highly infectious pathogen that represents a significant burden on the current healthcare system. Bacterial attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in chronic diseases such as endocarditis, osteomyelitis and wound infections. These biofilms decrease bacterial susceptibility to antibiotics and immune defences, making the infections challenging to treatment. S. aureus produces numerous exotoxins that contribute to the pathogenesis of the bacteria...
December 7, 2023: BMC Microbiology
https://read.qxmd.com/read/38048926/efficacy-of-lysostaphin-functionalized-silicon-catheter-for-the-prevention-of-staphylococcus-aureus-biofilm
#33
JOURNAL ARTICLE
Jayalakshmi Jayakumar, Vivek Vinod, Thennavan Arumugam, Binulal Nelson Sathy, Lalitha Biswas, V Anil Kumar, Raja Biswas
Staphylococcus aureus readily forms biofilms on tissue and indwelling catheter surfaces. These biofilms are resistant to antibiotics. Consequently, effective prevention and treatment strategies against staphylococcal biofilms are actively being pursued over the past two decades. One of the proposed strategies involve the incorporation of antibiotics and antiseptics into catheters, however, a persistent concern regarding the possible emergence of antimicrobial resistance is associated with these medical devices...
December 2, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38048846/antibody-antibiotic-conjugate-targeted-therapy-for-orthopedic-implant-associated-intracellular-s-aureus-infections
#34
JOURNAL ARTICLE
Leilei Qin, Ning Hu, Yanhao Zhang, Jianye Yang, Liqun Zhao, Xiaokai Zhang, Yun Yang, Jinyong Zhang, Yinshuang Zou, Keyu Wei, Chen Zhao, Yujian Li, Hao Zeng, Wei Huang, Quanming Zou
INTRODUCTION: Treating orthopedic implant-associated infections, especially those caused by Staphylococcus aureus (S. aureus), remains a significant challenge. S. aureus has the ability to invade host cells, enabling it to evade both antibiotics and immune responses during infection, which may result in clinical treatment failures. Therefore, it is critical to identify the host cell type of implant-associated intracellular S. aureus infections and to develop a strategy for highly targeted delivery of antibiotics to the host cells...
December 3, 2023: Journal of Advanced Research
https://read.qxmd.com/read/38036851/methicillin-resistant-staphylococci-in-clinical-bovine-mastitis-occurrence-molecular-analysis-and-biofilm-production
#35
JOURNAL ARTICLE
Silvia Preziuso, Anna-Rita Attili, Vincenzo Cuteri
Staphylococcus aureus is an important pathogen that causes mastitis in cattle, and the emergence of methicillin-resistant S. aureus (MRSA) poses a threat to veterinary and human medicine. The aims of the study were to investigate the prevalence of MRSA and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from clinical mastitis, their ability to form biofilms, and the antimicrobial susceptibility of S. aureus strains. In addition, the Staphylococcal Cassette Chromosome mec (SCCmec) type, spa type and the presence of Panton-Valentine Leucocidin in MRSA were evaluated...
December 1, 2023: Veterinary Research Communications
https://read.qxmd.com/read/38023268/-piper-betle-extract-and-its-application-in-bovine-teat-dipping-solution-inhibit-and-eliminate-biofilms-in-bovine-mastitis-inducing-staphylococci
#36
JOURNAL ARTICLE
Paparwee Sungkatavat, Haemarat Khongkhai, Wilasinee Kanchana, Phirabhat Saengsawarng, Suthinee Sangkanu, Veeranoot Nissapatorn, Maria de Lourdes Pereira, Julalak Chorachoo Ontong, Watcharapong Mitsuwan
BACKGROUND AND AIM: Staphylococci, including Staphylococcus aureus , Staphylococcus chromogenes , and Staphylococcus haemolyticus , are significant bacteria that induce bovine mastitis, primarily because they can form biofilms in bovine teat canals. This study aimed to investigate the efficacy of Piper betle extract and a bovine teat dipping solution containing P. betle extract (BSP) against these mastitis-causing staphylococci. MATERIALS AND METHODS: BSP was prepared using P...
October 2023: Veterinary World
https://read.qxmd.com/read/38019993/photodynamic-toluidine-blue-gold-nanoconjugates-as-a-novel-therapeutic-for-staphylococcal-biofilms
#37
JOURNAL ARTICLE
Mohammad Okkeh, Lorenzo De Vita, Giovanna Bruni, Lavinia Doveri, Paolo Minzioni, Elisa Restivo, Maddalena Patrini, Piersandro Pallavicini, Livia Visai
Staphylococci are among the most frequent bacteria known to cause biofilm-related infections. Pathogenic biofilms represent a global healthcare challenge due to their high tolerance to antimicrobials. In this study, water soluble polyethylene glycol (PEG)-coated gold nanospheres (28 ppm) and nanostars (15 ppm) with electrostatically adsorbed photosensitizer (PS) Toluidine Blue O (TBO) ∼4 μM were successfully synthesized and characterized as PEG-GNPs@TBO and PEG-GNSs@TBO. Both nanoconjugates and the TBO 4 μM solution showed remarkable, if similar, antimicrobial photodynamic inactivation (aPDI) effects at 638 nm, inhibiting the formation of biofilms by two Staphylococcal strains: a clinical methicillin-resistant Staphylococcus aureus (MRSA) isolate and Staphylococcus epidermidis ( S...
November 16, 2023: RSC Advances
https://read.qxmd.com/read/38019707/increased-activity-of-%C3%AE-lactam-antibiotics-in-combination-with-carvacrol-against-mrsa-bacteremia-and-catheter-associated-biofilm-infections
#38
JOURNAL ARTICLE
Jian-Guo Li, Xiao-Feng Chen, Ting-Yin Lu, Jing Zhang, Shu-He Dai, Jian Sun, Ya-Hong Liu, Xiao-Ping Liao, Yu-Feng Zhou
β-Lactam antibiotics are the mainstay for the treatment of staphylococcal infections, but their utility is greatly limited by the emergence and rapid dissemination of methicillin-resistant Staphylococcus aureus (MRSA). Herein, we evaluated the ability of the plant-derived monoterpene carvacrol to act as an antibiotic adjuvant, revitalizing the anti-MRSA activity of β-lactam antibiotics. Increased susceptibility of MRSA to β-lactam antibiotics and significant synergistic activities were observed with carvacrol-based combinations...
November 29, 2023: ACS Infectious Diseases
https://read.qxmd.com/read/38003500/the-controversial-effect-of-antibiotics-on-methicillin-sensitive-s-aureus-a-comparative-in-vitro-study
#39
JOURNAL ARTICLE
Valeria C J Hackemann, Stefan Hagel, Klaus D Jandt, Jürgen Rödel, Bettina Löffler, Lorena Tuchscherr
Methicillin-sensitive Staphylococcus ( S. ) aureus (MSSA) bacteremia remains a global challenge, despite the availability of antibiotics. Primary treatments include β-lactam agents such as cefazolin and flucloxacillin. Ongoing discussions have focused on the potential synergistic effects of combining these agents with rifampicin or fosfomycin to combat infections associated with biofilm formation. Managing staphylococcal infections is challenging due to antibacterial resistance, biofilms, and S. aureus 's ability to invade and replicate within host cells...
November 14, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37974055/pyrogallol-impairs-staphylococcal-biofilm-formation-via-induction-of-bacterial-oxidative-stress
#40
JOURNAL ARTICLE
Katharina H C Roese, Christina Torlone, Lauren A Cooper, Lee Esposito, Amy M Deveau, Ursula S R Röse, Kristin M Burkholder
AIMS: To examine the effect of the phenolic compound pyrogallol on staphylococcal biofilm formation. METHODS AND RESULTS: In crystal violet biofilm assays, pyrogallol reduced biofilm formation in S. epidermidis ATCC 35984, S. epidermidis NRRL-B41021, S. aureus USA300 and S. aureus Newman, without significantly impairing bacterial viability. Pyrogallol-mediated impairment of biofilm formation was likely due to induction of bacterial oxidative stress, as its effect was greater in catalase-deficient versus WT S...
November 16, 2023: Journal of Applied Microbiology
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