keyword
https://read.qxmd.com/read/38474351/effect-of-polyphenols-on-inflammation-induced-by-membrane-vesicles-from-staphylococcus-aureus
#1
JOURNAL ARTICLE
Yukino Oura, Yuko Shimamura, Toshiyuki Kan, Shuichi Masuda
Staphylococcus aureus , a bacterium found on human skin, produces toxins and various virulence factors that can lead to skin infections such as atopic dermatitis. These toxins and virulence factors are carried in membrane vesicles (MVs), composed of the bacterium's own cell membranes, and are expected to reach host target cells in a concentrated form, inducing inflammation. This study investigated the effects of two polyphenols, (-)-epigallocatechin gallate (EGCG) and nobiletin (NOL), on the expression of S...
February 23, 2024: Cells
https://read.qxmd.com/read/38457402/photoimmuno-antimicrobial-therapy-for-staphylococcus-aureus-implant-infection
#2
JOURNAL ARTICLE
Bruce van Dijk, Sabrina Oliveira, J Fred F Hooning van Duyvenbode, F Ruben H A Nurmohamed, Vida Mashayekhi, Irati Beltrán Hernández, Jos van Strijp, Lisanne de Vor, Piet C Aerts, H Charles Vogely, Harrie Weinans, Bart C H van der Wal
INTRODUCTION: Implant infections caused by Staphylococcus aureus are responsible for high mortality and morbidity worldwide. Treatment of these infections can be difficult especially when bacterial biofilms are involved. In this study we investigate the potential of infrared photoimmunotherapy to eradicate staphylococcal infection in a mouse model. METHODS: A monoclonal antibody that targets Wall Teichoic Acid surface components of both S. aureus and its biofilm (4497-IgG1) was conjugated to a photosensitizer (IRDye700DX) and used as photoimmunotherapy in vitro and in vivo in mice with a subcutaneous implant pre-colonized with biofilm of Staphylococcus aureus...
2024: PloS One
https://read.qxmd.com/read/38456817/poly-d-amino-acid-nanoparticles-target-staphylococcal-growth-and-biofilm-disassembly-by-interfering-with-peptidoglycan-synthesis
#3
JOURNAL ARTICLE
Wenli Feng, Marco Chittò, Wensheng Xie, Qun Ren, Fang Liu, Xiaoxu Kang, Dongdong Zhao, Guofeng Li, Thomas Fintan Moriarty, Xing Wang
d-Amino acids are signals for biofilm disassembly. However, unexpected metabolic pathways severely attenuate the utilization of d-amino acids in biofilm disassembly, resulting in unsatisfactory efficiency. Herein, three-dimensional poly(d-amino acid) nanoparticles (NPs), which possess the ability to block intracellular metabolism, are constructed with the aim of disassembling the biofilms. The obtained poly(α- N -acryloyl-d-phenylalanine)- block -poly(β- N -acryloyl-d-aminoalanine NPs (denoted as FA NPs) present α-amino groups and α-carboxyl groups of d-aminoalanine on their surface, which guarantees that FA NPs can effectively insert into bacterial peptidoglycan (PG) via the mediation of PG binding protein 4 (PBP4)...
March 8, 2024: ACS Nano
https://read.qxmd.com/read/38445864/inhibition-of-multiple-staphylococcal-growth-states-by-a-small-molecule-that-disrupts-membrane-fluidity-and-voltage
#4
JOURNAL ARTICLE
Jamie L Dombach, Grace L Christensen, Samual C Allgood, Joaquin L J Quintana, Corrella S Detweiler
New molecular approaches to disrupting bacterial infections are needed. The bacterial cell membrane is an essential structure with diverse potential lipid and protein targets for antimicrobials. While rapid lysis of the bacterial cell membrane kills bacteria, lytic compounds are generally toxic to whole animals. In contrast, compounds that subtly damage the bacterial cell membrane could disable a microbe, facilitating pathogen clearance by the immune system with limited compound toxicity. A previously described small molecule, D66, terminates Salmonella enterica serotype Typhimurium ( S ...
March 6, 2024: MSphere
https://read.qxmd.com/read/38440091/scc-mec-transformation-requires-living-donor-cells-in-mixed-biofilms
#5
JOURNAL ARTICLE
Mais Maree, Yuri Ushijima, Pedro B Fernandes, Masato Higashide, Kazuya Morikawa
Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that has emerged through the horizontal acquisition of the staphylococcal cassette chromosome mec (SCC mec ). Previously, we showed that SCC mec from heat-killed donors can be transferred via natural transformation in biofilms at frequencies of 10-8 -10-7 . Here, we show an improved transformation assay of SCC mec with frequencies up to 10-2 using co-cultured biofilms with living donor cells. The Ccr- attB system played an important role in SCC mec transfer, and the deletion of ccrAB recombinase genes reduced the frequency ∼30-fold...
June 2024: Biofilm
https://read.qxmd.com/read/38434581/remodeling-of-paranasal-sinuses-mucosa-functions-in-response-to-biofilm-induced-inflammation
#6
REVIEW
Szczepan Kaliniak, Krzysztof Fiedoruk, Jakub Spałek, Ewelina Piktel, Bonita Durnaś, Stanisław Góźdź, Robert Bucki, Sławomir Okła
Rhinosinusitis (RS) is an acute (ARS) or chronic (CRS) inflammatory disease of the nasal and paranasal sinus mucosa. CRS is a heterogeneous condition characterized by distinct inflammatory patterns (endotypes) and phenotypes associated with the presence (CRSwNP) or absence (CRSsNP) of nasal polyps. Mucosal barrier and mucociliary clearance dysfunction, inflammatory cell infiltration, mucus hypersecretion, and tissue remodeling are the hallmarks of CRS. However, the underlying factors, their priority, and the mechanisms of inflammatory responses remain unclear...
2024: Journal of Inflammation Research
https://read.qxmd.com/read/38415865/effect-of-potassium-permanganate-on-staphylococcal-isolates-derived-from-the-skin-of-patients-with-atopic-dermatitis
#7
JOURNAL ARTICLE
Sigrid Lundgren, Andreas Sonesson
In atopic dermatitis (AD), Staphylococcus aureus frequently colonizes lesions, leading to superinfections that can then lead to exacerbations. The presence of biofilm-producing isolates has been associated with worsening of the disease. Potassium permanganate is used as a topical treatment of infected eczema, blistering conditions, and wounds. Little is known of its effects against microbes in AD skin. The aim of this study was to explore antibacterial and antibiofilm properties of potassium permanganate against staphylococcal isolates derived from AD skin...
February 28, 2024: Acta Dermato-venereologica
https://read.qxmd.com/read/38415646/increased-production-of-aureolysin-and-staphopain-a-is-a-primary-determinant-of-the-reduced-virulence-of-staphylococcus-aureus-sara-mutants-in-osteomyelitis
#8
JOURNAL ARTICLE
Mara J Campbell, Karen E Beenken, Aura M Ramirez, Mark S Smeltzer
We previously demonstrated that mutation of sarA in Staphylococcus aureus limits biofilm formation, cytotoxicity for osteoblasts and osteoclasts, and virulence in osteomyelitis, and that all of these phenotypes can be attributed to the increased production of extracellular proteases. Here we extend these studies to assess the individual importance of these proteases alone and in combination with each other using the methicillin-resistant USA300 strain LAC, the methicillin-susceptible USA200 strain UAMS-1, and isogenic sarA mutants that were also unable to produce aureolysin (Aur), staphopain A (ScpA), staphylococcal serine protease A (subsp...
February 28, 2024: MBio
https://read.qxmd.com/read/38413623/thwarting-resistance-mgra-inhibition-with-methylophiopogonanone-a-unveils-a-new-battlefront-against-s-aureus
#9
JOURNAL ARTICLE
Xuerui Guo, Li Wang, Jinlong Zhang, Quan Liu, Bingmei Wang, Da Liu, Fei Gao, Gongga Lanzi, Yicheng Zhao, Yan Shi
Limitations in the clinical treatment of Staphylococcus aureus (S. aureus) infections have arisen due to the advent of antibiotic-resistant strains. Given the immense potential of therapeutic strategies targeting bacterial virulence, the role of MgrA as a pivotal virulence determinant in S. aureus-orchestrating resistance, adherence, and hundreds of virulence targets-becomes indispensable. In this investigation, leveraging advanced virtual screening and fluorescence anisotropy assays, we discerned methylophiopogonanone A (Mo-A), a flavonoid derivative, as a potent disruptor of the MgrA-DNA interaction nexus...
February 27, 2024: NPJ Biofilms and Microbiomes
https://read.qxmd.com/read/38387121/feasibility-of-using-bacteriophage-therapy-to-treat-staphylococcal-aureus-fracture-related-infections
#10
JOURNAL ARTICLE
James B Doub, Ashley E Levack, Lauren Sands, Joseph Blommer, Joseph Fackler, Robert V O'Toole
OBJECTIVE: Staphylococcus aureus fracture-related infections (FRIs) are associated with significant morbidity in part because conventional antibiotic therapies have limited ability to eradicate S. aureus in sessile states. Therefore, the objective of this study was to assess the feasibility of using Staphylococcal bacteriophages for FRI by testing the activity of a library of Staphylococcal bacteriophage therapeutics against historically preserved S. aureus FRI clinical isolates. METHODS: Current Procedural Terminology codes were used to identify patients with FRI from January 1, 2021 to December 31, 2021...
February 18, 2024: Injury
https://read.qxmd.com/read/38373661/effectiveness-of-ya-samarn-phlae-in-diabetic-wound-healing-evidence-from-in-vitro-studies-and-a-multicenter-randomized-controlled-clinical-trial
#11
JOURNAL ARTICLE
Sineenart Sanpinit, Julalak Chokpaisarn, Pinanong Na-Phatthalung, Dennapa Saeloh Sotthibandhu, Kanyatorn Yincharoen, Palika Wetchakul, Surasak Limsuwan, Sasitorn Chusri
ETHNOPHARMACOLOGICAL RELEVANCE: Ya-Samarn-Phlae (YaSP) has traditionally been widely used in southern Thailand for treating chronic and infected wounds, including diabetic foot ulcers. However, there are only a limited number of clinical studies supporting the use of this polyherbal formulation. Therefore, the present work aims to provide clinical evidence to support the application of YaSP, prepared according to a standardized traditional procedure (T-YaSP). Additionally, its potential chemical markers and wound healing-related biological activities were examined...
February 17, 2024: Journal of Ethnopharmacology
https://read.qxmd.com/read/38371297/resistome-mobilome-and-virulome-explored-in-clinical-isolates-derived-from-acne-patients-in-egypt-unveiling-unique-traits-of-an-emerging-coagulase-negative-staphylococcus-pathogen
#12
JOURNAL ARTICLE
Mai A Amer, Manal M Darwish, Noha S Soliman, Heba M Amin
Coagulase-negative staphylococci (CoNS) are a group of gram-positive staphylococcal species that naturally inhabit the healthy human skin and mucosa. The clinical impact of CoNS-associated infections has recently been regarded as a challenge for diagnosis and therapeutic options. CoNS-associated infections are primarily caused by bacterial resistance to antibiotics and biofilm formation. As antibiotics are still the most used treatment, this problem will likely persist in the future. The present study aimed to investigate the resistance and virulence of CoNS recovered from various acne lesions and explore their genetic basis...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38338807/abietic-acid-as-a-novel-agent-against-ocular-biofilms-an-in-vitro-and-preliminary-in-vivo-investigation
#13
JOURNAL ARTICLE
Monika Dzięgielewska, Marzenna Bartoszewicz, Marta Książczyk, Bartłomiej Dudek, Malwina Brożyna, Patrycja Szymczyk-Ziółkowska, Piotr Gruber, Jacek Pawlak, Weronika Kozłowska, Sylwia Zielińska, Jędrzej Fischer, Aleksandra Woytoń, Adam Junka
Biofilm-related ocular infections can lead to vision loss and are difficult to treat with antibiotics due to challenges with application and increasing microbial resistance. In turn, the design and testing of new synthetic drugs is a time- and cost-consuming process. Therefore, in this work, for the first time, we assessed the in vitro efficacy of the plant-based abietic acid molecule, both alone and when introduced to a polymeric cellulose carrier, against biofilms formed by Staphylococcus aureus , Pseudomonas aeruginosa , and Candida albicans in standard laboratory settings as well as in a self-designed setting using the topologically challenging surface of the artificial eye...
January 26, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38332421/virulence-determinants-and-antibiotic-resistance-in-staphylococci-isolated-from-the-skin-of-captive-bred-reptiles
#14
JOURNAL ARTICLE
Viola Strompfová, Lucia Štempelová, Dobroslava Bujňáková, Lívia Karahutová, Mária Nagyová, Leonard Siegfried
Knowledge of the composition and properties of skin microbiota in healthy reptiles is essential for preservation strategies and thus the prevention of skin dysbiosis leading to dermatological diseases. Despite the greatly increasing popularity of reptiles as pets, only a few studies have dealt with this topic. Therefore, the aim of this work was to analyse species composition of bacteria isolated from skin swabs of 40 reptiles (17 species) using MALDI-TOF spectrometry and to characterise the virulence properties of identified staphylococci (n = 51)...
February 9, 2024: Veterinary Research Communications
https://read.qxmd.com/read/38330222/in-vitro-and-in-vivo-assessment-of-the-infection-resistance-and-biocompatibility-of-small-molecule-modified-polyurethane-biomaterials
#15
JOURNAL ARTICLE
Li-Chong Xu, Jennifer L Booth, Matthew Lanza, Tugba Ozdemir, Amelia Huffer, Chen Chen, Asma Khursheed, Dongxiao Sun, Harry R Allcock, Christopher A Siedlecki
Bacterial intracellular nucleotide second messenger signaling is involved in biofilm formation and regulates biofilm development. Interference with the bacterial nucleotide second messenger signaling provides a novel approach to control biofilm formation and limit microbial infection in medical devices. In this study, we tethered small-molecule derivatives of 4-arylazo-3,5-diamino-1 H -pyrazole on polyurethane biomaterial surfaces and measured the biofilm resistance and initial biocompatibility of modified biomaterials in in vitro and in vivo settings...
February 8, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38329365/antimicrobial-and-anti-biofilm-activity-of-a-thiazolidinone-derivative-against-staphylococcus-aureus-in-vitro-and-in-vivo
#16
JOURNAL ARTICLE
Rui Zhao, Bingyu Du, Yue Luo, Fen Xue, Huanhuan Wang, Di Qu, Shiqing Han, Simon Heilbronner, Yanfeng Zhao
Staphylococcus aureus ( S. aureus ) causes many infections with significant morbidity and mortality. S. aureus can form biofilms, which can cause biofilm-associated diseases and increase resistance to many conventional antibiotics, resulting in chronic infection. It is critical to develop novel antibiotics against staphylococcal infections, particularly those that can kill cells embedded in biofilms. This study aimed to investigate the bacteriocidal and anti-biofilm activities of thiazolidinone derivative (TD-H2-A) against S...
February 8, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38296834/extracellular-g-quadruplexes-and-z-dna-protect-biofilms-from-dnase-i-and-g-quadruplexes-form-a-dnazyme-with-peroxidase-activity
#17
JOURNAL ARTICLE
Gabriel Antonio Salvador Minero, Andreas Møllebjerg, Celine Thiesen, Mikkel Illemann Johansen, Nis Pedersen Jørgensen, Victoria Birkedal, Daniel Erik Otzen, Rikke Louise Meyer
Many bacteria form biofilms to protect themselves from predators or stressful environmental conditions. In the biofilm, bacteria are embedded in a protective extracellular matrix composed of polysaccharides, proteins and extracellular DNA (eDNA). eDNA most often is released from lysed bacteria or host mammalian cells, and it is the only matrix component most biofilms appear to have in common. However, little is known about the form DNA takes in the extracellular space, and how different non-canonical DNA structures such as Z-DNA or G-quadruplexes might contribute to its function in the biofilm...
January 31, 2024: Nucleic Acids Research
https://read.qxmd.com/read/38273276/synergistic-use-of-anti-inflammatory-ketorolac-and-gentamicin-to-target-staphylococcal-biofilms
#18
JOURNAL ARTICLE
Amita Sekar, Dmitry Gil, Peyton Tierney, Madeline McCanne, Vikram Daesety, Darina Trendafilova, Orhun K Muratoglu, Ebru Oral
BACKGROUND: While antibiotics remain our primary tools against microbial infection, increasing antibiotic resistance (inherent and acquired) is a major detriment to their efficacy. A practical approach to maintaining or reversing the efficacy of antibiotics is the use of other commonly used therapeutics, which show synergistic antibacterial action with antibiotics. Here, we investigated the extent of antibacterial synergy between the antibiotic gentamicin and the anti-inflammatory ketorolac regarding the dynamics of biofilm growth, the rate of acquired resistance, and the possible mechanism of synergy...
January 25, 2024: Journal of Translational Medicine
https://read.qxmd.com/read/38262493/a-trinuclear-zn-ii-schiff-base-dicyanamide-complex-attenuates-bacterial-biofilm-formation-by-ros-generation-and-membrane-damage-and-exhibits-anticancer-activity
#19
JOURNAL ARTICLE
Mamata Barua, Shrabasti Bandyopadhyay, Abdul Wasai, Mrinmoy Ghosh, Indrani Roy, Pameli Ghosh, Subratanath Koner, Corrado Rizzoli, Adhiraj Roy, Sandip Saha, Supratim Mandal
A trinuclear Zn (II) complex, [(ZnL{N(CN)2 })2 Zn], termed complex 1 has been synthesized by the reaction of an aqueous solution of sodium dicyanamide to the methanolic solution of Zn (CH3 COO)2 , 2H2 O and corresponding Schiff base (H2 L) which is derived from 1:2 condensation of 1, 4 butane diamine with 3-ethoxy salicylaldehyde. Complex 1 is characterized by elemental analysis, IR, UV and Single X-ray diffraction study. Drug resistance is a growing global public health concern that has prompted researchers to look into advanced alternative treatment modalities...
January 21, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38258017/exploring-the-complexity-of-the-interaction-between-t-rubrum-and-s-aureus-s-epidermidis-in-the-formation-of-polymicrobial-biofilms
#20
JOURNAL ARTICLE
Jenyffie A Belizario, Níura M Bila, Carolina O Vaso, Caroline B Costa-Orlandi, Matheus B Mendonça, Ana M Fusco-Almeida, Regina H Pires, Maria José S Mendes-Giannini
Dermatophytes associated with bacteria can lead to severe, difficult-to-treat infections and contribute to chronic infections. Trichophyton rubrum , Staphylococcus aureus , and Staphylococcus epidermidis can form biofilms influenced by nutrient availability. This study investigated biofilm formation by these species by utilizing diverse culture media and different time points. These biofilms were studied through scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), biomass, metabolic activity, and colony-forming units (CFUs)...
January 18, 2024: Microorganisms
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