keyword
MENU ▼
Read by QxMD icon Read
search

Clusters

keyword
https://www.readbyqxmd.com/read/28535647/-effects-of-overexpression-of-mir-17-92-gene-cluster-on-the-biological-characteristics-of-prostate-cancer-cells-and-its-mechanism
#1
P Zhou, L Ma, J Zhou, J J Xu, F Liu, F Guo
Objective: To explore the effect and mechanism of over-expression of miR-17-92 gene cluster on the biological characteristics of prostate cancer cells. Methods: DU145 cells were transfected with miR-17-92 gene expression plasmid and clones with stable ectopic miR-17-92 overexpression were established. The cell viabilities of DU145-17-92 and DU145-control cells were monitored by xCELLigence system. Cell proliferation and apoptosis were analyzed by Ki-67 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay...
May 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28535478/the-effect-of-billboard-design-specifications-on-driving-a-pilot-study
#2
Hadas Marciano, Pe'erly Setter
Decades of research on the effects of advertising billboards on road accident rates, driver performance, and driver visual scanning behavior, has produced no conclusive findings. We suggest that road safety researchers should shift their focus and attempt to identify the billboard characteristics that are most distracting to drivers. This line of research may produce concrete guidelines for permissible billboards that would be likely to reduce the influence of the billboards on road safety. The current study is a first step towards this end...
May 20, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28535377/mechanism-of-ska-recruitment-by-ndc80-complexes-to-kinetochores
#3
Paweł Ł Janczyk, Katarzyna A Skorupka, John G Tooley, Daniel R Matson, Cortney A Kestner, Thomas West, Owen Pornillos, P Todd Stukenberg
Yeast use the ring-shaped Dam1 complex to slide down depolymerizing microtubules to move chromosomes, but current models suggest that other eukaryotes do not have a sliding ring. We visualized Ndc80 and Ska complexes on microtubules by electron microscopic tomography to identify the structure of the human kinetochore-microtubule attachment. Ndc80 recruits the Ska complex so that the V shape of the Ska dimer interacts along protofilaments. We identify a mutant of the Ndc80 tail that is deficient in Ska recruitment to kinetochores and in orienting Ska along protofilaments in vitro...
May 22, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28535195/presence-of-type-i-f-crispr-cas-systems-is-associated-with-antimicrobial-susceptibility-in-escherichia-coli
#4
Seyid Aydin, Yoann Personne, Enas Newire, Rebecca Laverick, Oliver Russell, Adam P Roberts, Virve I Enne
Background: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated cas genes are sequence-specific DNA nuclease systems found in bacteria and archaea. CRISPR/Cas systems use RNA transcripts of previously acquired DNA (spacers) to target invading genetic elements with the same sequence, including plasmids. In this research we studied the relationship between CRISPR/Cas systems and multidrug resistance in Escherichia coli . Methods: The presence of Type I-E and Type I-F CRISPR systems was investigated among 82 antimicrobial-susceptible and 96 MDR clinical E...
May 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28535188/molecular-heterogeneity-at-the-network-level-high-dimensional-testing-clustering-and-a-tcga-case-study
#5
Nicolas Städler, Frank Dondelinger, Steven M Hill, Rehan Akbani, Yiling Lu, Gordon B Mills, Sach Mukherjee
Motivation: Molecular pathways and networks play a key role in basic and disease biology. An emerging notion is that networks encoding patterns of molecular interplay may themselves differ between contexts, such as cell type, tissue or disease (sub)type. However, while statistical testing of differences in mean expression levels has been extensively studied, testing of network differences remains challenging. Furthermore, since network differences could provide important and biologically interpretable information to identify molecular subgroups, there is a need to consider the unsupervised task of learning subgroups and networks that define them...
May 23, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28535153/high-energy-particle-beam-and-gamma-radiation-exposure-familial-relatedness-and-cancer-in-mice
#6
Pavel Chernyavskiy, Elijah F Edmondson, Michael M Weil, Mark P Little
BACKGROUND: Some highly penetrant familial cancer syndromes exhibit elevated leukaemia risk, and there is evidence for familial clustering of lung cancer and other common cancers. Lung cancer and leukaemia are strongly radiogenic, but there are few indications that high-energy beam irradiation is markedly more effective than lower-energy radiation. METHODS: We used a Cox model with familially structured random effects to assess 16 mortality end points in a group of 1850 mice in 47 families maintained in a circular-breeding scheme, exposed to accelerated Si or Fe ions (0...
May 23, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28535120/differentiation-of-recurrent-glioblastoma-from-delayed-radiation-necrosis-by-using-voxel-based-multiparametric-analysis-of-mr-imaging-data
#7
Ra Gyoung Yoon, Ho Sung Kim, Myeong Ju Koh, Woo Hyun Shim, Seung Chai Jung, Sang Joon Kim, Jeong Hoon Kim
Purpose To assess a volume-weighted voxel-based multiparametric (MP) clustering method as an imaging biomarker to differentiate recurrent glioblastoma from delayed radiation necrosis. Materials and Methods The institutional review board approved this retrospective study and waived the informed consent requirement. Seventy-five patients with pathologic analysis-confirmed recurrent glioblastoma (n = 42) or radiation necrosis (n = 33) who presented with enlarged contrast material-enhanced lesions at magnetic resonance (MR) imaging after they completed concurrent chemotherapy and radiation therapy were enrolled...
May 23, 2017: Radiology
https://www.readbyqxmd.com/read/28535078/complementation-of-ctb7-in-the-maize-pathogen-cercospora-zeina-overcomes-the-lack-of-in-vitro-cercosporin-production
#8
Velushka Swart, Bridget Genevieve Crampton, John Ridenour, Burton Bluhm, Nicholas Olivier, Marion Meyer, Dave Kenneth Berger
Gray leaf spot (GLS), caused by the sibling species Cercospora zeina or Cercospora zeae-maydis, is cited as one of the most important diseases threatening global maize production. C. zeina fails to produce cercosporin in vitro, and in most cases causes large coalescing lesions during maize infection; a symptom generally absent from cercosporin-deficient mutants in other Cercospora spp. Here we describe the C. zeina cercosporin toxin biosynthetic gene cluster. The oxidoreductase gene CTB7 contained several insertions and deletions as compared to the C...
May 23, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28535010/microrna%C3%A2-141-inhibits-the-self%C3%A2-renewal-of-glioblastoma-stem-cells-via-jagged1
#9
Xianfeng Gao, Xiaobo Zhu, Yang Sun, Jingwei Liu
Glioblastoma multiforme is one of the most lethal types of brain cancer. With limited success from conventional therapies, the cancer stem cell theory was developed, and investigation into microRNAs (miRs) has facilitated understanding of this theory. The present study demonstrated that miR‑141 is suppressed in sorted cluster of differentiation (CD) 133(+) glioblastoma stem cells (GSCs) compared with CD133(‑) non‑glioblastoma stem cells (NSCs) from patient samples. In addition, miR‑141 overexpression inhibited the sphere formation ability of GSCs in vitro and in vivo...
May 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534985/microarray-analysis-of-differentially-expressed-genes-in-l929-mouse-fibroblast-cells-exposed-to-leptin-and-hypoxia
#10
Ping Ouyang, Sen Wang, He Zhang, Zhigang Huang, Pei Wei, Ye Zhang, Zhuguo Wu, Tao Li
Leptin and hypoxia are pro-fibrotic factors involved in fibrogenesis, however, the gene expression profiles remain to be fully elucidated. The aim of the present study was to investigate the regulatory roles of leptin and hypoxia on the L929 mouse fibroblast cell line. The cells were assigned to a normoxia, normoxia with leptin, hypoxia, and hypoxia with leptin group. The cDNA expression was detected using an Agilent mRNA array platform. The differentially expressed genes (DEGs) in response to leptin and hypoxia were identified using reverse transcription‑quantitative polymerase chain reaction analysis, followed by clustering analysis, Gene Ontology analysis and pathway analysis...
May 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534931/leucine-rich-glioma-inactivated-3-integrative-analyses-support-its-role-in-the-cytokine-network
#11
Hyun A Kim, Nyoun Soo Kwon, Kwang Jin Baek, Dong-Seok Kim, Hye-Young Yun
Leucine-rich glioma inactivated (LGI)3 is a secreted protein member of LGI family. We previously repo-rted that LGI3 was upregulated in adipose tissues from obese mice and suppressed adipogenesis through its receptor, a disintegrin and metalloproteinase domain-containing protein 23 (ADAM23). We demonstrated that LGI3 regulated tumor necrosis factor-α and adiponectin, and proposed that LGI3 may be a pro-inflammatory adipokine involved in adipose tissue inflammation. In this study, we analyzed adipokine and cytokine profiles in LGI3 knockout mice and demonstrated that multiple factors were increased or decreased in the adipose tissues and plasma of the LGI3 knockout mice...
May 15, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28534880/a-tale-of-two-risks-smoking-diabetes-and-the-subgingival-microbiome
#12
Sukirth M Ganesan, Vinayak Joshi, Megan Fellows, Shareef M Dabdoub, Haikady N Nagaraja, Benjamin O'Donnell, Neeta Rohit Deshpande, Purnima S Kumar
Although smoking and diabetes have been established as the only two risk factors for periodontitis, their individual and synergistic impacts on the periodontal microbiome are not well studied. The present investigation analyzed 2.7 million 16S sequences from 175 non-smoking normoglycemic individuals (controls), smokers, diabetics and diabetic smokers with periodontitis as well as periodontally healthy controls, smokers and diabetics to assess subgingival bacterial biodiversity and co-occurrence patterns. The microbial signatures of periodontally healthy smokers, but not diabetics, were highly aligned with the disease-associated microbiomes of their respective cohorts...
May 23, 2017: ISME Journal
https://www.readbyqxmd.com/read/28534836/differentiation-and-structure-in-sulfolobus-islandicus-rod-shaped-virus-populations
#13
Maria A Bautista, Jesse A Black, Nicholas D Youngblut, Rachel J Whitaker
In the past decade, molecular surveys of viral diversity have revealed that viruses are the most diverse and abundant biological entities on Earth. In culture, however, most viral isolates that infect microbes are represented by a few variants isolated on type strains, limiting our ability to study how natural variation affects virus-host interactions in the laboratory. We screened a set of 137 hot spring samples for viruses that infect a geographically diverse panel of the hyperthemophilic crenarchaeon Sulfolobus islandicus...
May 19, 2017: Viruses
https://www.readbyqxmd.com/read/28534793/an-information-theoretic-cluster-visualization-for-self-organizing-maps
#14
Leonardo Enzo Brito da Silva, Donald C Wunsch
Improved data visualization will be a significant tool to enhance cluster analysis. In this paper, an information-theoretic-based method for cluster visualization using self-organizing maps (SOMs) is presented. The information-theoretic visualization (IT-vis) has the same structure as the unified distance matrix, but instead of depicting Euclidean distances between adjacent neurons, it displays the similarity between the distributions associated with adjacent neurons. Each SOM neuron has an associated subset of the data set whose cardinality controls the granularity of the IT-vis and with which the first- and second-order statistics are computed and used to estimate their probability density functions...
May 17, 2017: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/28534786/evolutionary-computing-enriched-computer-aided-diagnosis-system-for-diabetic-retinopathy-a-survey
#15
Romany Mansour
The alterations caused due to diabetes mellitus results into significant micro-vasculature that eventually causes diabetic retinopathy (DR) that keeps on increasing as per time and eventually causes complete vision loss. Identifying subtle variations in morphological changes in retinal blood vessels, optic disk, exudates, micro-aneurysms, hemorrhage etc is complicate and requires robust computer aided diagnosis (CAD) system so as to enable earlier and efficient DR diagnosis practices. In majority of the existing CAD systems, the need of functional enhancement has been realized time by time to ensure accurate and time efficient DR...
May 17, 2017: IEEE Reviews in Biomedical Engineering
https://www.readbyqxmd.com/read/28534775/learning-rotation-invariant-local-binary-descriptor
#16
Yueqi Duan, Jiwen Lu, Jianjiang Feng, Jie Zhou
In this paper, we propose a rotation-invariant local binary descriptor (RI-LBD) learning method for visual recognition. Compared with hand-crafted local binary descriptors such as LBP and its variants which require strong prior knowledge, local binary feature learning methods are more efficient and dataadaptive. Unlike existing learning-based local binary descriptors such as compact binary face descriptor (CBFD) and simultaneous local binary feature learning and encoding (SLBFLE) which are susceptible to rotations, our RI-LBD first categorizes each local patch into a rotational binary pattern (RBP), and then jointly learns the orientation for each pattern and the projection matrix to obtain rotation-invariant local binary descriptors...
May 16, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28534619/quantitative-solid-state-nmr-study-on-ligand-surface-interaction-in-cysteine-capped-cdse-magic-sized-clusters
#17
Takuya Kurihara, Yasuto Noda, Kiyonori Takegoshi
Ligand-surface interaction of semiconductor nanoparticles (NPs) controls their optoelectronic properties, and thus examinations of the interaction are essential for the nanoelectronic applications of NPs. Herein, solid-state nuclear magnetic resonance (NMR) is performed to unravel the ligand-surface interaction in cysteine-capped CdSe magic-sized clusters. (15)N-(113)Cd through-bond J-filtered NMR directly shows the presence of the nitrogen-cadmium chemical bond for the first time and indicates that ~43% of the amines form the chemical bond...
May 23, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28534615/origins-of-water-molecules-in-the-photosystem-ii-crystal-structure
#18
Naoki Sakashita, Hiroshi C Watanabe, Takuya Ikeda, Keisuke Saito, Hiroshi Ishikita
The cyanobacterial photosystem II (PSII) crystal structure includes more than 1300 water molecules in each monomer unit; however, their precise roles in water oxidation are unclear. To understand the origins of water molecules in the PSII crystal structure, the accessibility of bulk water molecules to channel inner spaces in PSII was investigated using the water-removed PSII structure and molecular dynamics (MD) simulations. The inner space of the channel that proceeds toward the D1-Glu65/D2-Glu312 pair (E65/E312 channel) was entirely filled with water molecules from the bulk region...
May 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/28534584/composition-dependent-electronic-energy-relaxation-dynamics-of-metal-domains-as-revealed-by-bimetallic-au144-xagx-sc8h9-60-monolayer-protected-clusters
#19
Hongjun Zheng, Marcus A Tofanelli, Christopher J Ackerson, Kenneth L Knappenberger
We examined the electronic relaxation dynamics for mono and bimetallic Au144-xAgx(SC8H9)60 monolayer-protected clusters (MPCs) using femtosecond time-resolved transient extinction spectroscopy. MPCs provide compositionally well-defined model systems for structure-specific determination of nanoscale electronic properties. Based on pulse-energy-dependent transient extinction data, we quantified electron-phonon coupling constants for three distinct Au144-xAgx(SC8H9)60 MPC samples, where x = 0, 0 < x < 30, and x ∼ 30, as Gx=0 = (1...
May 23, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28534574/syntheses-structures-and-magnetic-properties-of-a-family-of-end-on-azido-bridged-cu-ii-ln-iii-complexes
#20
Xing-Cai Huang, Xin-Hua Zhao, Dong Shao, Xin-Yi Wang
The reaction of Cu(valdmpn) (H2valdmpn = N,N'-bis-(3-methoxysalicylidene)-1,3-diamino-2,2-dimethylpropane), LnCl3·nH2O (Ln = La(1), Ce (2), Pr(3), Nd(4), Sm(5), Eu(6), Gd(7), Tb(8), Dy(9), Ho(10), Er(11), Tm(12), Yb(13), Lu(14)) and NaN3 in methanol led to a whole series of heterometallic Cu(II)-Ln(III) complexes. The basic structural components of these complexes are the [Cu(II)(valdmpn)Ln(III)] units with a compartmental Schiff-base ligand. These [Cu(II)Ln(III)] units were then further connected by the end-on (EO) azides between the Ln(3+) centers to form tetranuclear {[CuLn](μ-N3)n[LnCu]} motifs, where n is 3 for the complexes of larger radii lanthanides (1CuLa to 5CuSm) and 2 for the complexes of smaller radii lanthanides (6CuEu to 14CuLu)...
May 23, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
keyword
keyword
40372
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"