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https://www.readbyqxmd.com/read/29471511/sexual-dimorphism-in-the-behavioral-responses-and-the-immunoendocrine-status-in-d-galactose-induced-aging
#1
Raquel Baeta-Corral, Rafael Castro-Fuentes, Lydia Giménez-Llort
For almost twenty years, chronic systemic D-galactose, a monosaccharide abundantly present in milk products, fruits and vegetables, has been used as a tool to achieve models of accelerated aging. Its neurotoxicity, induced by abnormal accumulation of ROS and AGEs, has been widely reported. However, behavioral outcomes are still controversial and little is known about sex-dependent vulnerability. We performed a comprehensive behavioral and multi-functional screening of the chronic effects of low (50 mg/kg) and high (100 mg/kg) doses of D-galactose in 6-month-old male and female gold-standard C57BL/6 mice...
February 19, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29471156/sliding-hip-screw-versus-cannulated-cancellous-screws-for-fixation-of-femoral-neck-fracture-in-adults-a-systematic-review
#2
REVIEW
Jian-Xiong Ma, Ming-Jie Kuang, Fei Xing, Yun-Long Zhao, Heng-Ting Chen, Lu-Kai Zhang, Zheng-Rui Fan, Chao Han, Xin-Long Ma
OBJECTIVE: Femoral neck fracture is considered a difficult fracture to treat and often gives rise to unsatisfactory treatment results. Cannulated cancellous screws (CCS) or a sliding hip screw (SHS) are the mainstream internal fixations used for osteosynthesis of femoral neck fractures. There is a need to integrate existing data through a meta-analysis to investigate the safety and effectiveness of CCS and SHS in the treatment of femoral neck fractures. METHOD: According to the Cochrane Handbook for Systematic Reviews of Interventions, we screened for the relevant studies by searching Google Scholar, the Cochrane Controlled Trials Register, the Cochrane Library, Web of Science, EMBASE, and PubMed...
February 19, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29471045/prdm14-a-putative-histone-methyl-transferase-interacts-with-and-decreases-the-stability-and-activity-of-the-hoxa1-transcription-factor
#3
Amandine Draime, Laure Bridoux, Magali Belpaire, Tamara Pringels, Janne Tys, René Rezsohazy
Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently co-expressed with HOXA1 in epiblast cells before their specification towards somatic versus germ cell fate. Here, we confirm PRDM14 is an interactor of HOXA1 and we identify the homeodomain of HOXA1 as well as the PR domain and Zinc fingers of PRDM14 to be required for the interaction...
February 19, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29470979/transcriptional-information-revealed-differentially-expressed-circular-rnas-in-facet-joint-osteoarthritis
#4
Chen Chu, Wu Chunshuai, Chen Jiajia, Zhang Jinlong, Xue Pengfei, Jiang Jiawei, Cui Zhiming
Circular RNAs are newly identified RNAs that regulate gene expression and execute various biological activities. Facet joint osteoarthritis (FJOA) is a severe facet joint cartilage degeneration disease that induces chronic low back pain to a larger population. The molecular mechanisms of FJOA, especially the presence and changes of circular RNAs in FJOA have not been investigated. In the current study, we analyzed RNA deep sequencing outcomes from healthy control group and FJOA group, identified and annotated circular RNAs, and determined the expressions of circular RNAs...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29470858/genes-affecting-progression-of-bacteriophage-p22-infection-in-salmonella-identified-by-transposon-and-single-gene-deletion-screens
#5
Kaitlynne Bohm, Steffen Porwollik, Weiping Chu, John A Dover, Eddie B Gilcrease, Sherwood R Casjens, Michael McClelland, Kristin N Parent
Bacteriophages rely on their hosts for replication, and many host genes critically determine either viral progeny production or host success via phage resistance. A random insertion transposon library of 240,000 mutants in Salmonella enterica serovar Typhimurium was used to monitor effects of individual bacterial gene disruptions on bacteriophage P22 lytic infection. These experiments revealed candidate host genes that alter the timing of phage P22 propagation. Using a False Discovery Rate of <0.1, mutations in 235 host genes either blocked or delayed progression of P22 lytic infection, including many genes for which this role was previously unknown...
February 22, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29470815/identification-of-mir-9-as-a-negative-factor-of-insulin-secretion-from-beta-cells
#6
Dongzhi Hu, Yi Wang, Haiyang Zhang, Dalu Kong
MicroRNA is a novel class of small noncoding RNA that has been implicated in a variety of physiological and pathological processes, including glucose homeostasis and diabetes mellitus. So far, a few studies have reported that miRNAs may be an important regulator in glucose-stimulated insulin secretion (GSIS) pathway. However, the role of miRNAs in this process remains unclear. The levels of miRNAs in mouse islets and MIN6 cells were determined by quantitative RT-PCR. Concentration of insulin was determined by ELISA, and the expression of the target protein was determined with western blot assay...
February 22, 2018: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29470693/anti-inflammatory-anti-rheumatic-and-analgesic-activities-of-2-5-mercapto-1-3-4-oxadiazol-2-yl-n-propylbenzenesulphonamide-mopbs-in-rodents
#7
Hina Rasheed, Ruqayya Afridi, Ashraf Ullah Khan, Muhammad Zia Ullah, Sidra Khalid, Ayesha Atiq, Humaira Kashif, Muhammad Naeem Ahmed, Yeong Shik Kim, Salman Khan
Chronic inflammation is pathologically associated with various clinical conditions such as rheumatoid arthritis. Several anti-inflammatory and analgesic drugs currently available in market presents a wide range of problems. Therefore, the current study was aimed to evaluate anti-inflammatory and analgesic activities of newly synthesized compound 2-(5-mercapto-1,3,4-oxadiazol-2-yl)-N-propylbenzenesulphonamide (MOPBS). Carrageenan and CFA-induced models were developed for evaluation of anti-inflammatory and analgesic activity...
February 22, 2018: Inflammopharmacology
https://www.readbyqxmd.com/read/29470529/xenopus-an-alternative-model-system-for-identifying-muco-active-agents
#8
Hyo Jung Sim, Sang-Hyun Kim, Kyung-Jae Myung, Taejoon Kwon, Hyun-Shik Lee, Tae Joo Park
The airway epithelium in human plays a central role as the first line of defense against environmental contaminants. Most respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma, and respiratory infections, disturb normal muco-ciliary functions by stimulating the hypersecretion of mucus. Several muco-active agents have been used to treat hypersecretion symptoms in patients. Current muco-active reagents control mucus secretion by modulating either airway inflammation, cholinergic parasympathetic nerve activities or by reducing the viscosity by cleaving crosslinking in mucin and digesting DNAs in mucus...
2018: PloS One
https://www.readbyqxmd.com/read/29470517/two-essential-thioredoxins-mediate-apicoplast-biogenesis-protein-import-and-gene-expression-in-toxoplasma-gondii
#9
Marco Biddau, Anne Bouchut, Jack Major, Tracy Saveria, Julie Tottey, Ojore Oka, Marcel van-Lith, Katherine Elizabeth Jennings, Jana Ovciarikova, Amy DeRocher, Boris Striepen, Ross Frederick Waller, Marilyn Parsons, Lilach Sheiner
Apicomplexan parasites are global killers, being the causative agents of diseases like toxoplasmosis and malaria. These parasites are known to be hypersensitive to redox imbalance, yet little is understood about the cellular roles of their various redox regulators. The apicoplast, an essential plastid organelle, is a verified apicomplexan drug target. Nuclear-encoded apicoplast proteins traffic through the ER and multiple apicoplast sub-compartments to their place of function. We propose that thioredoxins contribute to the control of protein trafficking and of protein function within these apicoplast compartments...
February 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29470392/gja4-connexin-37-mutations-correlate-with-secondary-lymphedema-following-surgery-in-breast-cancer-patients
#10
Mahrooyeh Hadizadeh, Seiied Mojtaba Mohaddes Ardebili, Mansoor Salehi, Chris Young, Fariborz Mokarian, James McClellan, Qin Xu, Mohammad Kazemi, Elham Moazam, Behzad Mahaki, Maziar Ashrafian Bonab
Lymphedema is a condition resulting from mutations in various genes essential for lymphatic development and function, which leads to obstruction of the lymphatic system. Secondary lymphedema is a progressive and incurable condition, most often manifesting after surgery for breast cancer. Although its causation appears complex, various lines of evidence indicate that genetic predisposition may play a role. Previous studies show that mutations in connexin 47 are associated with secondary lymphedema. We have tested the hypothesis that connexin 37 gene mutations in humans are associated with secondary lymphedema following breast cancer surgery...
February 22, 2018: Biomedicines
https://www.readbyqxmd.com/read/29470291/recurrent-abdominal-pain-in-children-summary-evidence-from-3-systematic-reviews-of-treatment-effectiveness
#11
Rebecca A Abbott, Alice E Martin, Tamsin V Newlove-Delgado, Alison Bethel, Rebecca S Whear, Jo Thompson Coon, Stuart Logan
OBJECTIVES: Between 4% and 25% of school-aged children complain of recurrent abdominal pain (RAP) severe enough to interfere with their daily activities. METHODS: We carried out a systematic review of randomised controlled trials (RCTs) in eleven databases and 2 trials registries from inception to June 2016. An update search was run in November 2017. All screening was performed by 2 independent reviewers. Included studies were appraised using the Cochrane risk of bias tool and the evidence assessed using GRADE...
February 21, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29470105/diabetes-mellitus-m-health-applications-a-systematic-review-of-features-and-fundamentals
#12
Ericles Andrei Bellei, Daiana Biduski, Nathália Pinto Cechetti, Ana Carolina Bertoletti De Marchi
BACKGROUND: The increasing number of diabetes mellitus (DM) m-health applications (apps) reveals a panorama of different approaches to the subject, demonstrating dynamism and heterogeneity. Available applications have various functions, capabilities, and data collection techniques. OBJECTIVE: We systematically reviewed the literature to identify and analyze studies regarding the diversity of applications aimed at DM monitoring and treatment. We aimed to investigate app functionalities, the basis for their design, and how they were tested...
February 22, 2018: Telemedicine Journal and E-health: the Official Journal of the American Telemedicine Association
https://www.readbyqxmd.com/read/29469876/visible-infrared-imaging-radiometer-suite-reflective-solar-bands-on-orbit-calibration-using-solar-diffuser-illuminated-by-scattered-light-through-the-nadir-port
#13
Junqiang Sun, Mike Chu, Menghua Wang
A new variant to the standard on-orbit calibration of the reflective solar bands (RSBs) using a solar diffuser (SD) is formulated. Instead of direct solar exposure through the SD port in the front of the instrument as originally designed, the variant method uses light reflecting off Earth's surface coming through the nadir port as the light source to illuminate the built-in onboard SD. The methodology is applied to the Visible Infrared Imaging Radiometer Suite on board the Suomi National Polar-orbiting Partnership satellite, and is shown to be viable and useful...
February 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/29469452/-preparation-of-recombinant-retrovirus-prevtre-e77-43-and-its-protective-effect-in-a-mouse-model-of-schistosoma-japonicum-infection
#14
Zeng Fan-Sheng, Luo Sai-Qun, Xiong De-Hui, Yu Yuan-Jing, Qian Ying-Jun, Qin Zhi-Qiang
OBJECTIVE: To explore the biological functions of E77.43, a gene segment of Microtus fortis , in treating Schistosoma japonicum infection. METHODS: Recombinant retroviral vectors of pRevTRE - E77.43 was constructed, and recombinant retroviral vectors were transfected into PA317 cells, and the stable cell lines were obtained by hygromycin screening, followed by the packaging, concentration and purification of recombinant retrovirus. The virus was transferred to the mice infected by S...
December 27, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469196/the-relationship-between-inflammation-and-remodeling-in-childhood-asthma-a-systematic-review
#15
REVIEW
Jose A Castro-Rodriguez, Sejal Saglani, Carlos E Rodriguez-Martinez, Maria A Oyarzun, Louis Fleming, Andrew Bush
OBJECTIVES: We aimed to perform a systematic review of all studies with direct measurements of both airway inflammation and remodeling in the subgroup of children with repeated wheezing and/or persistent asthma severe enough to warrant bronchoscopy, to address whether airway inflammation precedes remodeling or is a parallel process, and also to assess the impact of remodeling on lung function. METHODS: Four databases were searched up to June 2017. Two independent reviewers screened the literature and extracted relevant data...
February 22, 2018: Pediatric Pulmonology
https://www.readbyqxmd.com/read/29469138/x-ray-transparent-microfluidic-platforms-for-membrane-protein-crystallization-with-microseeds
#16
Jeremy M Schieferstein, Ashtamurthy S Pawate, Michael J Varel, Sudipto Guha, Ieva Astrauskaite, Robert B Gennis, Paul J A Kenis
Crystallization of membrane proteins is a critical step for uncovering atomic resolution 3-D structures and elucidating structure-function relationships. Microseeding, the process of transferring sub-microscopic crystal nuclei from initial screens into new crystallization experiments, is an effective, yet underutilized approach to grow crystals suitable for X-ray crystallography. Here, we report simplified methods for crystallization of membrane proteins that utilize microseeding in X-ray transparent microfluidic chips...
February 22, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29468878/identification-of-a-novel-esterase-from-marine-environmental-genomic-dna-libraries-and-its-application-in-production-of-free-all-trans-astaxanthin
#17
Ping Lu, Xinwei Gao, Hao Dong, Zhen Liu, Francesco Secundo, Changhu Xue, Xiangzhao Mao
Astaxanthin is a pigment with various functions. Free astaxanthin is obtained mainly through saponification methods, which could result in many by-products. Enzymatic methods using lipases have been used in few cases, while there are no reports on the use of esterases for the production of free astaxanthin. Herein we present the screening and identification of a novel esterase (Est3-14) from a marine mud metagenomic library. Est3-14 is pH-sensitive and keeps good stability in akaline buffers (residual activity 94%, pH 8...
February 22, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29468712/new-insights-in-type-i-and-ii-cd20-antibody-mechanisms-of-action-with-a-panel-of-novel-cd20-antibodies
#18
Saskia Meyer, Mitchell Evers, Johannes H M Jansen, Jos Buijs, Blanca Broek, Stephanie E Reitsma, Petra Moerer, Mojtaba Amini, Anna Kretschmer, Toine Ten Broeke, Marcel T den Hartog, Mark Rijke, Christian Klein, Thomas Valerius, Peter Boross, Jeanette H W Leusen
Based on their mechanisms-of-action, CD20 monoclonal antibodies (mAbs) are grouped into Type I [complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC)] and Type II [programmed cell death (PCD) and ADCC] mAbs. We generated 17 new hybridomas producing CD20 mAbs of different isotypes and determined unique heavy and light chain sequence pairs for 13 of them. We studied their epitope binding, binding kinetics and structural properties and investigated their predictive value for effector functions, i...
February 22, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29468521/identification-of-a-constitutively-active-mutant-mouse-irak2-by-retroviral-expression-screening
#19
Yanmei Liu, Weilan Yin, Lingqing Xu, Helin Zhang, Qian Liu, Weiguo Yin
To identify the importance of IRAK2 kinase activity in TLR-mediated signaling pathways, we constructed a retroviral vector harboring either a mouse IRAK2 gene (IRAK2-WT) or with its mutant with loss of function of its ATP-binding site (IRAK2-KD). Further, we comparatively analyzed for the gain of function and modulations in TLR-mediated signaling pathways in IRAK2 knockout (IRAK2-KO) macrophages upon introduction of the IRAK2-WT retroviral constructs. The pBS/IRAK2-KD with the ATP-binding site mutation in IRAK2 was obtained by using site-specific mutagenesis...
February 21, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29468433/merging-perspectives-genotype-directed-molecular-therapy-for-hereditary-diffuse-gastric-cancer-hdgc-and-e-cadherin-egfr-crosstalk
#20
Dandan Li, Winifred Lo, Udo Rudloff
Hereditary diffuse gastric cancer is a cancer predisposition syndrome associated with germline mutations of the E-cadherin gene (CDH1; NM_004360). Male CDH1 germline mutation carriers have by the age of 80 years an estimated 70% cumulative incidence of gastric cancer, females of 56% for gastric and of 42% for lobular breast cancer. Metastatic HDGC has a poor prognosis which is worse than for sporadic gastric cancer. To date, there have been no treatment options described tailored to this molecular subtype of gastric cancer...
February 22, 2018: Clinical and Translational Medicine
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