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https://www.readbyqxmd.com/read/29691654/the-association-between-25-hydroxyvitamin-d-concentration-physical-performance-and-frailty-status-in-older-adults
#1
Anouk M M Vaes, Elske M Brouwer-Brolsma, Nicole Toussaint, Margot de Regt, Michael Tieland, Luc J C van Loon, Lisette C P G M de Groot
PURPOSE: Sufficient 25-hydroxyvitamin D (25(OH)D) concentrations might prevent a decline in physical performance, and are considered important for the prevention of frailty. This study investigates the association of serum 25(OH)D concentration with physical performance and frailty status in Dutch older adults. METHODS: This cross-sectional study included 756 men and women, aged ≥ 65 years. Serum 25(OH)D concentration and frailty status (Fried criteria) were assessed in the total population...
April 25, 2018: European Journal of Nutrition
https://www.readbyqxmd.com/read/29691334/forward-genetic-dissection-of-biofilm-development-by-fusobacterium-nucleatum-novel-functions-of-cell-division-proteins-ftsx-and-envc
#2
Chenggang Wu, Abu Amar Mohamed Al Mamun, Truc Thanh Luong, Bo Hu, Jianhua Gu, Ju Huck Lee, Melissa D'Amore, Asis Das, Hung Ton-That
Fusobacterium nucleatum is a key member of the human oral biofilm. It is also implicated in preterm birth and colorectal cancer. To facilitate basic studies of fusobacterial virulence, we describe here a versatile transposon mutagenesis procedure and a pilot screen for mutants defective in biofilm formation. Out of 10 independent biofilm-defective mutants isolated, the affected genes included the homologs of the Escherichia coli cell division proteins FtsX and EnvC, the electron transport protein RnfA, and four proteins with unknown functions...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29691140/interaction-between-the-staphylococcus-aureus-extracellular-adherence-protein-eap-and-its-subdomains-with-platelets
#3
Raghavendra Palankar, Ulrike Binsker, Bianca Haracska, Jan Wesche, Andreas Greinacher, Sven Hammerschmidt
S. aureus associated bacteremia can lead to severe infections with high risk of mortality (e.g. sepsis, infective endocarditis). Many virulence factors and adhesins of S. aureus are known to directly interact with platelets. Extracellular adherence protein, Eap, one of the most important virulence factors in S. aureus mediated infections is a multi-tandem domain protein and has been shown to interact with almost all cell types in the human circulatory system. By using amine reactive fluorescent N-hydroxysuccinimidyl (NHS)-ester dyes and by direct detection with primary fluorescently conjugated anti-histidine (His-tag) antibodies against detect N-terminal His6, we show Eap subdomain Eap D3 D4 specifically interacts and rapidly activates human platelets...
April 18, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29690872/identification-of-a-neuronal-population-in-the-telencephalon-essential-for-fear-conditioning-in-zebrafish
#4
Pradeep Lal, Hideyuki Tanabe, Maximiliano L Suster, Deepak Ailani, Yuri Kotani, Akira Muto, Mari Itoh, Miki Iwasaki, Hironori Wada, Emre Yaksi, Koichi Kawakami
BACKGROUND: Fear conditioning is a form of learning essential for animal survival and used as a behavioral paradigm to study the mechanisms of learning and memory. In mammals, the amygdala plays a crucial role in fear conditioning. In teleost, the medial zone of the dorsal telencephalon (Dm) has been postulated to be a homolog of the mammalian amygdala by anatomical and ablation studies, showing a role in conditioned avoidance response. However, the neuronal populations required for a conditioned avoidance response via the Dm have not been functionally or genetically defined...
April 25, 2018: BMC Biology
https://www.readbyqxmd.com/read/29690522/coping-with-environmental-eukaryotes-identification-of-pseudomonas-syringae-genes-during-the-interaction-with-alternative-hosts-or-predators
#5
Federico Dorati, Glyn A Barrett, Maria Sanchez-Contreras, Tanya Arseneault, Mateo San José, David J Studholme, Jesús Murillo, Primitivo Caballero, Nicholas R Waterfield, Dawn L Arnold, Liz J Shaw, Robert W Jackson
Understanding the molecular mechanisms underpinning the ecological success of plant pathogens is critical to develop strategies for controlling diseases and protecting crops. Recent observations have shown that plant pathogenic bacteria, particularly Pseudomonas , exist in a range of natural environments away from their natural plant host e.g., water courses, soil, non-host plants. This exposes them to a variety of eukaryotic predators such as nematodes, insects and amoebae present in the environment. Nematodes and amoeba in particular are bacterial predators while insect herbivores may act as indirect predators, ingesting bacteria on plant tissue...
April 21, 2018: Microorganisms
https://www.readbyqxmd.com/read/29689640/haploid-genetic-screens-identify-genetic-vulnerabilities-to-microtubule-targeting-agents
#6
Nora M Gerhards, Vincent A Blomen, Merve Mutlu, Joppe Nieuwenhuis, Denise Howald, Charlotte Guyader, Jos Jonkers, Thijn R Brummelkamp, Sven Rottenberg
The absence of biomarkers to accurately predict anti-cancer therapy response remains a major obstacle in clinical oncology. We applied a genome-wide loss-of-function screening approach in human haploid cells to characterize genetic vulnerabilities to classical microtubule-targeting agents. Using docetaxel and vinorelbine, two well-established chemotherapeutic agents, we sought to identify genetic alterations sensitizing human HAP1 cells to these drugs. Despite the fact that both drugs act on microtubules, a set of distinct genes were identified whose disruption affect drug sensitivity...
April 24, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29689505/childhood-adversities-as-predictors-of-improvement-in-psychiatric-symptoms-and-global-functioning-in-solution-focused-and-short-and-long-term-psychodynamic-psychotherapy-during-a-5-year-follow-up
#7
Erkki Heinonen, Paul Knekt, Tommi Härkänen, Esa Virtala, Olavi Lindfors
BACKGROUND: Childhood adversities are frequent among adults who seek treatment for depression or anxiety. These disorders are commonly treated by psychotherapy. Yet it is not known if specific types or durations of psychotherapy are particularly suited for patients who have suffered various early adversities. METHODS: 221 depressed and anxious adult outpatients from community, student, occupational, and private healthcare services filled the Childhood Family Atmosphere Questionnaire...
April 7, 2018: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29689311/time-and-temperature-dependent-analytical-stability-of-dry-collected-evalyn-hpv-self-sampling-brush-for-cervical-cancer-screening
#8
Ditte Møller Ejegod, Helle Pedersen, Garazi Peña Alzua, Camilla Pedersen, Jesper Bonde
As a new initiative, HPV self-sampling to non-attenders using the dry Evalyn self-sampling brush is offered in the Capital Region of Denmark. The use of a dry brush is largely uncharted territory in terms of analytical stability. In this study we aim to provide evidence on the analytical quality of dry HPV self-sampling brushes as a function of time and temperature. We assessed the analytical stability of dry stored Evalyn brushes at three different temperatures, (4°C, room temperature, 30°C) and five different storage time points; T=0 (baseline), 2, 4, 8, 16, and 32 weeks prior to HPV analysis using the BD Onclarity HPV assay...
April 21, 2018: Papillomavirus Research
https://www.readbyqxmd.com/read/29689263/analysis-of-glycogen-metabolic-pathway-utilization-by-dendritic-cells-and-t-cells-using-custom-phenotype-metabolic-assays
#9
Phyu Thwe, Eyal Amiel
In the field of immunology, there is an increasing interest in cellular energy metabolism and its outcome on immune cell effector function. Activation of immune cells leads to rapid metabolic changes that are central to cellular biology in order to support the effector responses. Therefore, the need for user-friendly and dependable assay technologies to address metabolic regulation and nutrient utilization in immune cells is an important need in this field. Redox-dye reduction-based Phenotype MicroArray (PM) assays, which measure NADH reduction as a readout, developed by Biolog Inc...
April 21, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29689135/intravenous-thrombolysis-in-unwitnessed-stroke-onset-mr-witness-trial-results
#10
Lee H Schwamm, Ona Wu, Shlee S Song, Lawrence L Latour, Andria L Ford, Amie W Hsia, Alona Muzikansky, Rebecca A Betensky, Albert J Yoo, Michael H Lev, Gregoire Boulouis, Arne Lauer, Pedro Cougo, William A Copen, Gordon J Harris, Steven Warach
OBJECTIVE: Most acute ischemic stroke (AIS) patients with unwitnessed symptom onset are ineligible for intravenous thrombolysis due to timing alone. Lesion evolution on FLAIR MRI correlates with stroke duration, and quantitative mismatch of diffusion-weighted MRI (DWI) with FLAIR (qDFM) might indicate stroke duration within guideline-recommended thrombolysis. We tested if intravenous thrombolysis ≤4.5 h from time of symptom discovery is safe in patients with qDFM in an open-label, phase 2a, prospective study (NCT01282242)...
April 24, 2018: Annals of Neurology
https://www.readbyqxmd.com/read/29689111/inter-relationships-between-objective-handwriting-features-and-executive-control-among-children-with-developmental-dysgraphia
#11
Sara Rosenblum
OBJECTIVE: To describe handwriting and executive control features and their inter-relationships among children with developmental dysgraphia, in comparison to controls. METHOD: Participants included 64 children, aged 10-12 years, 32 with dysgraphia based on the Handwriting Proficiency Screening Questionnaire (HPSQ) and 32 matched controls. Children copied a paragraph onto paper affixed to a digitizer that supplied handwriting process objective measures (Computerized Penmanship Evaluation Tool (ComPET)...
2018: PloS One
https://www.readbyqxmd.com/read/29689073/liver-disease-burden-and-required-treatment-expenditures-for-hepatitis-c-virus-hcv-infection-in-thailand-implications-for-hcv-elimination-in-the-new-therapeutic-era-a-population-based-study
#12
Rujipat Wasitthankasem, Preeyaporn Vichaiwattana, Nipaporn Siripon, Nawarat Posuwan, Chompoonut Auphimai, Sirapa Klinfueng, Napha Thanetkongtong, Viboonsak Vuthitanachot, Supapith Saiyatha, Chaiwat Thongmai, Saowakon Sochoo, Natnada Pongsuwan, Kittiyod Poovorawan, Pisit Tangkijvanich, Yong Poovorawan
The prevalence of hepatitis C virus (HCV) infection has been decreasing globally, but the growing effects of HCV-related morbidity and mortality remain of concern. Advances in curative medicine, involving direct-acting antivirals (DAAs), have led many countries to aim to eradicate HCV. Information on epidemiology and disease burden is essential for national policy development. Thus, this study aimed to determine the HCV-related hepatic disease burden in areas of Thailand with high and average HCV prevalence in order to extrapolate the viral burden across Thailand...
2018: PloS One
https://www.readbyqxmd.com/read/29689051/the-epidemiology-of-personality-disorders-in-the-sao-paulo-megacity-general-population
#13
Geilson Lima Santana, Bruno Mendonca Coelho, Yuan-Pang Wang, Alexandre Dias Porto Chiavegatto Filho, Maria Carmen Viana, Laura Helena Andrade
INTRODUCTION: Most studies on the epidemiology of personality disorders (PDs) have been conducted in high-income countries and may not represent what happens in most part of the world. In the last decades, population growth has been concentrated in low- and middle-income countries, with rapid urbanization, increasing inequalities and escalation of violence. Our aim is to estimate the prevalence of PDs in the Sao Paulo Metropolitan Area, one of the largest megacities of the world. We examined sociodemographic correlates, the influence of urban stressors, the comorbidity with other mental disorders, functional impairment and treatment...
2018: PloS One
https://www.readbyqxmd.com/read/29688705/modularisation-and-response-curve-engineering-of-a-naringenin-responsive-transcriptional-biosensor
#14
Brecht De Paepe, Jo Maertens, Bartel Vanholme, Marjan De Mey
To monitor the intra- and extracellular environment of micro-organisms and to adapt their metabolic processes accordingly, scientists are reprogramming nature's myriad of transcriptional regulatory systems into transcriptional biosensors which are able to detect small molecules and, in response, express specific output signals of choice. However, the naturally occurring response curve, the key characteristic of biosensor circuits, is typically not in line with the requirements for real-life biosensor applications...
April 24, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29688014/machine-learning-energy-gaps-of-porphyrins-with-molecular-graph-representations
#15
Zheng Li, Noushin Omidvar, Wei Shan Chin, Esther Robb, Amanda J Morris, Luke E K Achenie, Hongliang Xin
Molecular functionalization of porphyrins opens countless new opportunities in tailoring their physicochemical properties for light-harvesting applications. However, the immense materials space spanned by a vast number of substituent ligands and chelating metal ions prohibits high-throughput screening of combinatorial libraries. In this work, machine learning algorithms equipped with the domain knowledge of chemical graph theory were employed for predicting the energy gaps of >12000 porphyrins from the Computational Materials Repository...
April 24, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29687846/screening-and-functional-analysis-of-micro-rnas-that-regulate-the-expression-of-the-tumor-suppression-tp53-gene
#16
L-K Li, Z-H Kong
OBJECTIVE: With gradual increase of cancer incidence and mortality rates, the regulatory mechanism of cancer has become a hotspot. It has been known that the expression of TP53 is closely associated with the occurrence of cancer. The microRNA (miRNA)-mediated regulation of the expression of numerous proto-oncogenes has been reported. This study aimed to identify miRNAs that regulate the expression of TP53 gene. MATERIALS AND METHODS: The sequence of TP53 gene was downloaded from NCBI and analyzed with TargetScan software to predict potential miRNAs that regulate TP53 expression...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29687495/circular-rna-hsa_circ_0008344-regulates-glioblastoma-cell-proliferation-migration-invasion-and-apoptosis
#17
Jinxu Zhou, Hongxiang Wang, Junsheng Chu, Qilin Huang, Guangxu Li, Yong Yan, Tao Xu, Juxiang Chen, Yuhai Wang
BACKGROUND: Recent studies have found circular RNAs (circRNAs) involved in the biological process of cancers. However, little is known about their functional roles in glioblastoma. METHODS: Human circRNA microarray analysis was performed to screen the expression profile of circRNAs in IDH1 wild-type glioblastoma tissue. The expression of hsa_circ_0008344 in glioblastoma and normal brain samples was quantified by qRT-PCR. Functional experiments were performed to investigate the biological functions of hsa_circ_0008344, including MTT assay, colony formation assay, transwell assay, and cell apoptosis assay...
April 24, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29687346/long-term-cognitive-outcomes-after-sepsis-a-translational-systematic-review
#18
REVIEW
Tatiana Barichello, Pavani Sayana, Vijayasree V Giridharan, Anithachristy S Arumanayagam, Boomadevi Narendran, Amanda Della Giustina, Fabricia Petronilho, João Quevedo, Felipe Dal-Pizzol
Sepsis is systemic inflammatory response syndrome with a life-threatening organ dysfunction that is caused by an unbalanced host immune response in an attempt to eliminate invasive microorganisms. We posed questions, "Does sepsis survivor patients have increased risk of neuropsychiatric manifestations?" and "What is the mechanism by which sepsis induces long-term neurological sequelae, particularly substantial cognitive function decline in survivor patients and in pre-clinical sepsis models?" The studies were identified by searching PubMed/MEDLINE (National Library of Medicine), PsycINFO, EMBASE (Ovid), LILACS (Latin American and Caribbean Health Sciences Literature), IBECS (Bibliographical Index in Spanish in Health Sciences), and Web of Science databases for peer-reviewed journals that were published until January 2018...
April 23, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29686973/microrna-4516-contributes-to-different-functions-of-epithelial-permeability-barrier-by-targeting-poliovirus-receptor-related-protein-1-in-enterovirus-71-and-coxsackievirus-a16-infections
#19
Yajie Hu, Jie Song, Longding Liu, Ying Zhang, Lichun Wang, Qihan Li
Enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) remain the predominant etiological agents of hand, foot, and mouth disease (HFMD). The clinical manifestations caused by the two viruses are obviously different. CV-A16 usually triggers a repeated infection, and airway epithelial integrity is often the potential causative factor of respiratory repeated infections. Our previous studies have demonstrated that there were some differentially expressed miRNAs involved in the regulation of adhesion function of epithelial barrier in EV-A71 and CV-A16 infections...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29686617/a-chimeric-nav1-8-channel-expression-system-based-on-hek293t-cell-line
#20
Xi Zhou, Yunxiao Zhang, Dongfang Tang, Songping Liang, Ping Chen, Cheng Tang, Zhonghua Liu
Among the nine voltage-gated sodium channel (NaV) subtypes, NaV1.8 is an attractive therapeutic target for pain. The heterologous expression of recombinant NaV1.8 currents is of particular importance for its electrophysiological and pharmacological studies. However, NaV1.8 expresses no or low-level functional currents when transiently transfected into non-neuronal cell lines. The present study aims to explore the molecular determinants limiting its functional expression and accordingly establish a functional NaV1...
2018: Frontiers in Pharmacology
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