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https://www.readbyqxmd.com/read/29220744/sensitive-detection-of-pyoverdine-with-an-electrochemical-sensor-based-on-electrochemically-generated-graphene-functionalized-with-gold-nanoparticles
#1
Islem Gandouzi, Mihaela Tertis, Andreea Cernat, Amina Bakhrouf, Maria Coros, Stela Pruneanu, Cecilia Cristea
The design and development of an electrochemical sensor for the sensitive and selective determination of pyoverdine, a virulence factor secreted by Pseudomonas aeruginosa, bacteria involved in nosocomial infections is presented in this work. The presence of pyoverdine in water and body fluids samples can be directly linked to the presence of the Pseudomonas bacteria, thus being a nontoxic and low cost marker for the detection of water pollution as well as for the biological contamination of other media. The sensor was elaborated using layer-by-layer technique for the deposition of a graphene‑gold nanoparticles composite film on the graphite-based screen printed electrode, from aqueous suspension...
December 5, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29220541/yoga-for-stroke-rehabilitation
#2
REVIEW
Maggie Lawrence, Francisco T Celestino Junior, Hemilianna Hs Matozinho, Lindsay Govan, Jo Booth, Jane Beecher
BACKGROUND: Stroke is a major health issue and cause of long-term disability and has a major emotional and socioeconomic impact. There is a need to explore options for long-term sustainable interventions that support stroke survivors to engage in meaningful activities to address life challenges after stroke. Rehabilitation focuses on recovery of function and cognition to the maximum level achievable, and may include a wide range of complementary strategies including yoga.Yoga is a mind-body practice that originated in India, and which has become increasingly widespread in the Western world...
December 8, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29218571/culturable-endophytic-bacteria-of-ginger-rhizome%C3%A2-and-their-remarkable-multi-trait-plant-growth-promoting-features
#3
Sabu Rohini, R Aswani, M Kannan, V P Sylas, E K Radhakrishnan
Functional contribution of endophytic bacteria towards plant growth is highly impressive due to their species diversity and array of probiotic mechanisms. In the study, 96 endophytic bacteria isolated from rhizome of ginger (Zingiber officinale) were screened for phosphate solubilisation, 1-amino cyclopropane-1-carboxylate (ACC) deaminase activity, nitrogen fixation, ammonia and IAA production. Among these, sixteen endophytes with multiple plant growth-promoting activities were identified by 16S rDNA sequencing and all of them showed growth enhancement in Vigna unguiculata var Lola which make the study remarkably significant...
December 7, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29218557/what-can-we-learn-from-wide-angle-solution-scattering
#4
Yujing Wang, Hao Zhou, Emre Onuk, John Badger, Lee Makowski
Extending collection of x-ray solution scattering data into the wide-angle regime (WAXS) can provide information not readily extracted from small angle (SAXS) data. It is possible to accurately predict WAXS scattering on the basis of atomic coordinate sets and thus use it as a means of testing molecular models constructed on the basis of crystallography, molecular dynamics (MD), cryo-electron microscopy or ab initio modeling. WAXS data may provide insights into the secondary, tertiary and quaternary structural organization of macromolecules...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29218522/a-growth-based-screening-system-for-hexose-transporters-in-yeast
#5
Eckhard Boles, Mislav Oreb
As the simplest eukaryotic model system, the unicellular yeast Saccharomyces cerevisiae is ideally suited for quick and simple functional studies as well as for high-throughput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29218430/cpdock-the-complementarity-plot-for-docking-of-proteins-implementing-multi-dielectric-continuum-electrostatics
#6
Sankar Basu
The complementarity plot (CP) is an established validation tool for protein structures, applicable to both globular proteins (folding) as well as protein-protein complexes (binding). It computes the shape and electrostatic complementarities (Sm, Em) for amino acid side-chains buried within the protein interior or interface and plots them in a two-dimensional plot having knowledge-based probabilistic quality estimates for the residues as well as for the whole structure. The current report essentially presents an upgraded version of the plot with the implementation of the advanced multi-dielectric functionality (as in Delphi version 6...
December 7, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29217678/hrpu-2-a-homologue-of-mammalian-hnrnp-u-regulates-synaptic-transmission-by-controlling-the-expression-of-slo-2-potassium-channel-in-c-elegans
#7
Ping Liu, Sijie Jason Wang, Zhao-Wen Wang, Bojun Chen
Slo2 channels are large-conductance potassium channels abundantly expressed in the nervous system. However, it is unclear how their expression level in neurons is regulated. Here we report that HRPU-2, an RNA-binding protein homologous to mammalian hnRNP U, plays an important role in regulating the expression of SLO-2 (a homologue of mammalian Slo2) in C. elegans Loss-of-function (lf) mutants of hrpu-2 were isolated in a genetic screen for suppressors of a sluggish phenotype caused by a hyperactive SLO-2. In hrpu-2(lf) mutants, SLO-2-mediated delayed outward currents in neurons are greatly decreased, and neuromuscular synaptic transmission is enhanced...
December 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29217655/regulation-of-lipolytic-response-and-energy-balance-by-melanocortin-2-receptor-accessory-protein-mrap-in-adipocytes
#8
Xiaodong Zhang, Alicia M Saarinen, Latoya E Campbell, Elena A De Filippis, Jun Liu
MRAP is highly expressed in adrenal gland and adipose tissue. In adrenal cells, MRAP is essential for ACTH-induced activation of the cAMP/PKA pathway by melanocortin 2 receptor (MC2R), leading to glucocorticoid production and secretion. Although ACTH was known to stimulate PKA-dependent lipolysis, the functional involvement of MRAP in adipocyte metabolism remains incompletely defined. Herein, we found that knockdown or overexpression of MRAP in 3T3-L1 adipocytes reduced or increased ACTH-induced lipolysis, respectively...
December 7, 2017: Diabetes
https://www.readbyqxmd.com/read/29217616/interactome-mapping-uncovers-a-general-role-for-numb-in-protein-kinase-regulation
#9
Ran Wei, Tomonori Kaneko, Xuguang Liu, Huadong Liu, Lei Li, Courtney Voss, Eric Liu, Ningning He, Shawn S C Li
Cellular functions are frequently regulated by protein-protein interactions involving the binding of a modular domain in one protein to a specific peptide sequence in another.  This mechanism may be explored to identify binding partners for proteins harboring a peptide-recognition domain. Here we report a proteomic strategy combining peptide and protein microarray screening with biochemical and cellular assays to identify modular domain-mediated protein-protein interactions in a systematic manner. We applied this strategy to Numb, a multi-functional protein containing a phosphotyrosine-binding (PTB) domain...
December 7, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29217301/-gut-microbiota-what-impact-on-colorectal-carcinogenesis-and-treatment
#10
REVIEW
Antoine Bruneau, Minh-Tam Baylatry, Anne Christine Joly, Harry Sokol
The gut microbiota, composed of 1014 microorganisms, is now considered as a "hidden organ", regarding to its digestive, metabolic and immune functions, which are helpful to its host. For the last 15 years, advances in molecular biology have highlighted the association of gut microbiota dysbiosis with several diseases, including colorectal cancer. An increased abundance of some bacteria (including Fusobacterium nucleatum, Bacteroides fragilis, Escherichia coli) is associated with cancer, whereas others seem to be protective (Faecalibacterium prausnitzii)...
December 4, 2017: Bulletin du Cancer
https://www.readbyqxmd.com/read/29217288/avelumab-in-metastatic-urothelial-carcinoma-after-platinum-failure-javelin-solid-tumor-pooled-results-from-two-expansion-cohorts-of-an-open-label-phase-1-trial
#11
Manish R Patel, John Ellerton, Jeffrey R Infante, Manish Agrawal, Michael Gordon, Raid Aljumaily, Carolyn D Britten, Luc Dirix, Keun-Wook Lee, Mathew Taylor, Patrick Schöffski, Ding Wang, Alain Ravaud, Arnold B Gelb, Junyuan Xiong, Galit Rosen, James L Gulley, Andrea B Apolo
BACKGROUND: The approval of anti-programmed death ligand 1 (PD-L1) and anti-programmed death 1 agents has expanded treatment options for patients with locally advanced or metastatic urothelial carcinoma. Avelumab, a human monoclonal anti-PD-L1 antibody, has shown promising antitumour activity and safety in this disease. We aimed to assess the safety profile in patients (both post-platinum therapy and cisplatin-naive) treated with avelumab and to assess antitumour activity of this drug in post-platinum patients...
December 4, 2017: Lancet Oncology
https://www.readbyqxmd.com/read/29217214/reducing-physical-violence-toward-primary-school-students-with-disabilities
#12
Karen Devries, Hannah Kuper, Louise Knight, Elizabeth Allen, Nambusi Kyegombe, Lena Morgon Banks, Susan Kelly, Dipak Naker
PURPOSE: We tested whether the Good School Toolkit reduces physical violence from peers and school staff toward students with and without disabilities in Ugandan primary schools. METHODS: We conducted a cluster randomized controlled trial, with data collected via cross-sectional surveys in 2012 and 2014. Forty-two primary schools in Luwero District, Uganda, were randomly assigned to receive the Good School Toolkit for 18 months, or to a waitlisted control group...
December 5, 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/29216977/noninvasive-cardiac-imaging-and-the-prediction-of-heart-failure-progression-in%C3%A2-preclinical-stage-a-b-subjects
#13
REVIEW
Fei Fei Gong, Duncan J Campbell, David L Prior
Heart failure (HF) continues to grow as a cause of morbidity and mortality in our community and presents a significant public health problem, predominantly in individuals ≥65 years of age. Early intervention in asymptomatic HF subjects (Stage A/B) at risk of progression to symptomatic HF (Stage C/D) may provide an opportunity to halt this epidemic. The ability of cardiac imaging to assess cardiac structure and function permits early identification of those at increased risk of developing symptomatic HF. Systolic, diastolic, and structural left ventricular parameters each predict symptomatic HF, but no single parameter has sufficient sensitivity for screening to identify individuals with Stage A/B HF who are at increased risk of disease progression...
December 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/29216914/rasch-analysis-of-the-participation-scale-p-scale-usefulness-of-the-p-scale-to-a-rehabilitation-services-network
#14
Mariana Angélica Peixoto Souza, Wendy Jane Coster, Marisa Cotta Mancini, Fabiana Caetano Martins Silva Dutra, Jessica Kramer, Rosana Ferreira Sampaio
BACKGROUND: A person's participation is acknowledged as an important outcome of the rehabilitation process. The Participation Scale (P-Scale) is an instrument that was designed to assess the participation of individuals with a health condition or disability. The scale was developed in an effort to better describe the participation of people living in middle-income and low-income countries. The aim of this study was to use Rasch analysis to examine whether the Participation Scale is suitable to assess the perceived ability to take part in participation situations by patients with diverse levels of function...
December 8, 2017: BMC Public Health
https://www.readbyqxmd.com/read/29216274/functional-analysis-of-a-cdkn2a-5-utr-germline-variant-associated-with-pancreatic-cancer-development
#15
William Bruno, Virginia Andreotti, Alessandra Bisio, Lorenza Pastorino, Giuseppe Fornarini, Stefania Sciallero, Giovanna Bianchi-Scarrà, Alberto Inga, Paola Ghiorzo
CDKN2A coding region germline variants are associated with pancreatic adenocarcinoma (PC) susceptibility. Recently, we described functional germline 5'UTR CDKN2A variants from melanoma patients affecting the post-transcriptional regulation of p16INK4a mRNA that is dependent, at least in part, on an Internal Ribosome Entry Site (IRES) in the 5'UTR region. Here we describe a 5'UTR c.-201_-198delinsCTTT CDKN2A variant (frequency 0.0028 based on 350 PC patients), which seems to be private to PC, since it has never been found in public databases nor in thousands of melanoma patients tested...
2017: PloS One
https://www.readbyqxmd.com/read/29216252/global-computational-mutagenesis-provides-a-critical-stability-framework-in-protein-structures
#16
Caitlyn L McCafferty, Yuri V Sergeev
A protein's amino acid sequence dictates the folds and final structure the macromolecule will form. We propose that by identifying critical residues in a protein's atomic structure, we can select a critical stability framework within the protein structure essential to proper protein folding. We use global computational mutagenesis based on the unfolding mutation screen to test the effect of every possible missense mutation on the protein structure to identify the residues that cannot tolerate a substitution without causing protein misfolding...
2017: PloS One
https://www.readbyqxmd.com/read/29215878/ultra-fast-screening-of-a-novel-moderate-hydrophilic-angiotensin-converting-enzyme-inhibitory-peptide-ryl-from-silkworm-pupa-using-fe-doped-silkworm-excrement-derived-biocarbon-waste-conversion-by-waste
#17
Long Liu, Yanan Wei, Qing Chang, Huaju Sun, Kungang Chai, Zuqiang Huang, Zhenxia Zhao, Zhongxing Zhao
A novel moderate hydrophilic peptide (RYL) with high ACE inhibitory activity was ultra-fast screened via a concept of waste conversion using waste. This novel peptide was screened from silkworm Pupa using Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement. FL/Z-SE possessed magnetic property and specific selection for peptides due to Fe's dual functions. The selected RYL with moderate hydrophilicity (LogP=-0.22) exhibited a comparatively high ACE inhibitory activity (IC<u>50</u>=3...
December 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29215864/platinum-nanocatalyst-amplification-redefining-the-gold-standard-for-lateral-flow-immunoassays-with-ultra-broad-dynamic-range
#18
Colleen N Loynachan, Michael R Thomas, Eleanor R Gray, Daniel A Richards, Jeongyun Kim, Benjamin S Miller, Jennifer Clare Brookes, Shweta Agarwal, Vijay Chudasama, Rachel A McKendry, Molly M Stevens
Paper-based lateral flow immunoassays (LFIAs) are one of the most widely used point-of-care (PoC) devices, however, their application in early disease diagnostics is often limited due to insufficient sensitivity for the requisite sample sizes and the short timeframes of PoC testing. To address this, we developed a serum-stable, nanoparticle catalyst-labelled LFIA with a sensitivity surpassing that of both current commercial and published sensitivities for paper-based detection of p24, one of the earliest and most conserved biomarkers of HIV...
December 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/29215701/long-non-coding-rna-lncrna-rmst-in-triple-negative-breast-cancer-tnbc-expression-analysis-and-biological-roles-research
#19
Li Wang, Dequan Liu, Xingrao Wu, Yueqin Zeng, Lan Li, Yu Hou, Wenhui Li, Zhijie Liu
OBJECTIVE: Microarray showed that lncRNA RMST was differentially expressed in cervical cancer. Further experiments were conducted to detect the expression and biological function of RMST in triple-negative breast cancer (TNBC). METHODS: Microarray was used to screen the differentially expressed lncRNAs in TNBC. QRT-PCR was applied to uncover the expression of RMST in TNBC tissues. The cell viability of RMST-transfected TNBC cells were probed by CKK-8 assay and colony formation assay...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29215565/a-miniaturized-impedimetric-immunosensor-for-the-competitive-detection-of-adrenocorticotropic-hormone
#20
Nan Li, Egor M Larin, Kagan Kerman
Adrenocorticotropic hormone (ACTH) plays an essential role in regulating corticosteroid hormone production, which has important functions in a myriad of critical physiological functions. In this proof-of-concept study, a miniaturized immunosensor was developed for the highly sensitive detection of ACTH using electrochemical impedance spectroscopy (EIS) in connection with disposable screen-printed gold electrodes (SPGEs). A film of 3,3'-dithiobis[sulfosuccinimidylpropionate] (DTSSP) was prepared to immobilize anti-ACTH antibodies covalently on the nanostructured SPGE surface...
December 7, 2017: Sensors
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