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https://www.readbyqxmd.com/read/28087360/the-synthetic-peptide-pnpp-19-induces-peripheral-antinociception-via-activation-of-no-cgmp-katp-pathway-role-of-enos-and-nnos
#1
A C N Freitas, G Silva, D F Pacheco, V Lemos, I D G Duarte, M E de Lima
BACKGROUND AND PURPOSE: The peptide PnPP-19, derived from the spider toxin PnTx2-6, potentiates erectile function by activating the nitrergic system. Since NO has been studied as an antinociceptive molecule and PnPP-19 is known to induce peripheral antinociception, we intended to evaluate whether PnPP-19 could induce peripheral antinociception through activation of this pathway. EXPERIMENTAL APPROACH: Nociceptive thresholds were measured by paw pressure test. PGE2 (2 μg/paw) was administered intraplantarly together with PnPP-19 and inhibitors/blockers of NOS, guanylyl cyclase and KATP channels...
January 10, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28076881/constitutive-nos-uncoupling-and-nadph-oxidase-upregulation-in-the-penis-of-type-2-diabetic-men-with-erectile-dysfunction
#2
B Musicki, A L Burnett
Erectile dysfunction (ED) associated with type 2 diabetes mellitus (T2DM) involves dysfunctional nitric oxide (NO) signaling and increased oxidative stress in the penis. However, the mechanisms of endothelial NO synthase (eNOS) and neuronal NO synthase (nNOS) dysregulation, and the sources of oxidative stress, are not well defined, particularly at the human level. The objective of this study was to define whether uncoupled eNOS and nNOS, and NADPH oxidase upregulation, contribute to the pathogenesis of ED in T2DM men...
January 11, 2017: Andrology
https://www.readbyqxmd.com/read/28045993/change-in-the-interstitial-cells-of-cajal-and-nnos-positive-neuronal-cells-with-aging-in-the-stomach-of-f344-rats
#3
Yong Hwan Kwon, Nayoung Kim, Ryoung Hee Nam, Ji Hyun Park, Sun Min Lee, Sung Kook Kim, Hye Seung Lee, Yong Sung Kim, Dong Ho Lee
The gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxide synthase (nNOS) and the function changes in the corpus of F344 rats at different ages (6-, 31-, 74-wk and 2-yr). The proportion of the c-Kit-positive area in the submucosal border (SMB) and myenteric plexus (MP) layer was significantly lower in the older rats, as indicated by immunohistochemistry...
2017: PloS One
https://www.readbyqxmd.com/read/28034782/spinal-trpc6-channels-contributes-to-morphine-induced-antinociceptive-tolerance-and-hyperalgesia-in-rats
#4
Hua Jin, Ying-Tong Sun, Guang-Qiong Guo, Da-Lin Chen, Yu-Jin Li, Gao-Peng Xiao, Xin-Nan Li
The chronic administration of opioids results in the development of morphine analgesic tolerance and withdrawl-induced hyperalgesia, which limits their clinical utility in pain treatment. However, the cellular mechanisms underlying opioid-induced tolerance and hyperalgesia are not fully understood. The transient receptor potential canonical channel TRPC6 is important for brain development and function, as it regulates cytosolic, endoplasmic reticulum, and mitochondrial Ca(2+) levels in neural cells. Here, we report that TRPC6 expression in the spinal cord was up-regulated after chronic morphine treatment...
December 26, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/28028361/characterization-of-smooth-muscle-enteric-nerve-interstitial-cells-of-cajal-and-fibroblast-like-cells-in-the-gastric-musculature-of-patients-with-diabetes-mellitus
#5
Kyung Sik Park, Kwang Bum Cho, Il Seon Hwang, Jae Hyung Park, Byung Ik Jang, Kyeong Ok Kim, Sung Woo Jeon, Eun Soo Kim, Chang Sik Park, Joong Goo Kwon
AIM: To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM). METHODS: Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson's Trichrome stain were performed to assess the degree of fibrosis. Immunohistochemical staining using various antibodies was also performed [antibodies against protein gene product 9.5 (PGP9.5), neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), neurokinin-1 (NK1) receptor, c-Kit, and platelet-derived growth factor receptor-alpha, (PDGFRα)]...
December 14, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28025407/altered-s-nitrosylation-of-p53-is-responsible-for-impaired-antioxidant-response-in-skeletal-muscle-during-aging
#6
Sara Baldelli, Maria Rosa Ciriolo
p53 transcriptional activity has been proposed to regulate both homeostasis and sarcopenia of skeletal muscle during aging. However, the exact molecular function of p53 remains to be clearly defined. We demonstrated a requirement of nuclear p53 S-nitrosylation in inducing a nitric oxide/PGC-1α-mediated antioxidant pathway in skeletal muscle. Importantly, mutant form of p53-DNA binding domain (C124S) did not undergo nuclear S-nitrosylation and failed in inducing the expression of antioxidant genes (i.e. SOD2 and GCLC)...
December 20, 2016: Aging
https://www.readbyqxmd.com/read/28012927/role-of-scoparia-dulcis-linn-on-noise-induced-nitric-oxide-synthase-nos-expression-and-neurotransmitter-assessment-on-motor-function-in-wistar-albino-rats
#7
Wankupar Wankhar, Sakthivel Srinivasan, Loganathan Sundareswaran, Dapkupar Wankhar, Ravindran Rajan, Rathinasamy Sheeladevi
Noise pollution is one of the most widespread and fast growing environmental and occupational menaces in the modern era. Exposure to noise above 100dB is not adaptable through the brain homeostatic mechanism. Yet, the detrimental effects of noise have often been ignored. Developing reliable animal models to understand the neurobiology of noise stress and advance our research in the field of medicine to impede this growing stressor is needed. In this study experimental animals were divided into four groups, (i) Control and (ii) S...
December 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28007537/human-umbilical-cord-mesenchymal-stem-cells-transplantation-improves-cognitive-function-in-alzheimer-s-disease-mice-by-decreasing-oxidative-stress-and-promoting-hippocampal-neurogenesis
#8
YuanBo Cui, ShanShan Ma, ChunYan Zhang, Wei Cao, Min Liu, DongPeng Li, PengJu Lv, Qu Xing, RuiNa Qu, Ning Yao, Bo Yang, FangXia Guan
Stem cell transplantation represents a promising therapy for central nervous system injuries, but its application to Alzheimer's disease (AD) is still limited and the potential mechanism for cognition improvement remains to be elucidated. In the present study, we used Tg2576 mice which express AD-like pathological forms of amyloid precursor protein (APP) to investigate the effects of human umbilical cord mesenchymal stem cells (hUC-MSCs) intravenous transplantation on AD mice. Interestingly, hUC-MSCs transplantation significantly ameliorated cognitive function of AD mice without altering Aβ levels in hippocampus...
December 19, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/28006954/the-neuroprotective-effect-of-dimethyl-fumarate-in-a-mptp-mouse-model-of-parkinson-s-disease-involvement-of-reactive-oxygen-species-nuclear-factor-%C3%AE%C2%BAb-nuclear-transcription-factor-related-to-nf-e2
#9
Michela Campolo, Giovanna Casili, Flavia Biundo, Rosalia Crupi, Marika Cordaro, Salvatore Cuzzocrea, Emanuela Esposito
AIM: Oxidative stress plays a key role in Parkinson's disease (PD) and nuclear transcription factor related to NF-E2 (Nrf-2) is involved in neuroprotection against PD. The aim of the present study was to investigate the neurotherapeutic action, NF-κB/Nrf-2 dependent, of DMF in a mouse model of PD and in vitro SHSY-5Y cells. RESULTS: Daily oral gavage of DMF (10, 30 and 100 mg/kg) significantly reduced neuronal degeneration of dopaminergic tract and behavioral impairments induced by four injections of the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)...
December 23, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28004072/-temporal-and-spatial-changes-of-%C3%AE-7nachr-and-nnos-in-cerebral-cortex-and-hippocampus-of-a%C3%AE-induced-cognitive-dysfunction-rats
#10
Yan-Li Wang, Li-Xia Liu, Shao-Hu Wang, Wen-Xiu Qi
The present study was to explore the temporal and spatial distributions and variations of α7 nicotinic acetylcholine receptor (α7nAChR) and neuronal nitric oxide synthetase (nNOS) in cerebral cortex and hippocampus of Aβ-induced cognitive dysfunction rats. Sixty Sprague-Dawley (SD) rats were randomly divided into six groups. Three experimental groups were intracerebroventricularly (i.c.v.) injected with condensed-amyloid beta peptides 1-42 (Aβ1-42, 2.5 µg/µL, 4 µL) and were observed on day 7 (7 d Aβ group), day 14 (14 d Aβ group) and day 21 (21 d Aβ group), respectively...
December 25, 2016: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/27994632/calycosin-and-formononetin-induce-endothelium-dependent-vasodilation-by-the-activation-of-large-conductance-ca-2-activated-k-channels-bkca
#11
Hisa Hui Ling Tseng, Chi Teng Vong, George Pak-Heng Leung, Sai Wang Seto, Yiu Wa Kwan, Simon Ming-Yuen Lee, Maggie Pui Man Hoi
Calycosin and formononetin are two structurally similar isoflavonoids that have been shown to induce vasodilation in aorta and conduit arteries, but study of their actions on endothelial functions is lacking. Here, we demonstrated that both isoflavonoids relaxed rat mesenteric resistance arteries in a concentration-dependent manner, which was reduced by endothelial disruption and nitric oxide synthase (NOS) inhibition, indicating the involvement of both endothelium and vascular smooth muscle. In addition, the endothelium-dependent vasodilation, but not the endothelium-independent vasodilation, was blocked by BKCa inhibitor iberiotoxin (IbTX)...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27992968/establishment-of-in%C3%A2-vitro-model-of-erectile-dysfunction-for-the-study-of-high-glucose-induced-angiopathy-and-neuropathy
#12
G N Yin, S-H Park, K-M Song, A Limanjaya, K Ghatak, N N Minh, J Ock, J-K Ryu, J-K Suh
Penile erection requires complex interaction between vascular endothelial cells, smooth muscle cells, pericytes, and autonomic nerves. Diabetes mellitus is one of the most common causes of erectile dysfunction (ED) and multiple pathogenic factors, such as cavernous angiopathy and autonomic neuropathy, are associated with diabetic ED. Although a variety of animal models of diabetic ED play an important role in understanding pathophysiologic mechanisms of diabetes-induced ED, these animal models have limitations for addressing the exact cellular or molecular mechanisms involved in ED...
December 19, 2016: Andrology
https://www.readbyqxmd.com/read/27989753/the-tetrahydrobiopterin-radical-with-high-and-low-spin-heme-in-neuronal-nitric-oxide-synthase-a-new-indicator-of-the-extent-of-nos-coupling
#13
Matthew D Krzyaniak, Alex A Cruce, Preethi Vennam, Molly Lockart, Vladimir Berka, Ah-Lim Tsai, Michael K Bowman
Reaction intermediates trapped during the single-turnover reaction of the neuronal ferrous nitric oxide synthase oxygenase domain (Fe(II)nNOSOX) show four EPR spectra of free radicals. Fully-coupled nNOSOX with cofactor (tetrahydrobiopterin, BH4) and substrate (l-arginine) forms the typical BH4 cation radical with an EPR spectrum ~4.0 mT wide and hyperfine tensors similar to reports for a biopterin cation radical in inducible NOSOX (iNOSOX). With excess thiol, nNOSox lacking BH4 and l-arg is known to produce superoxide...
October 28, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/27987021/impaired-contraction-and-decreased-detrusor-innervation-in-a-female-rat-model-of-pelvic-neuropraxia
#14
Johanna L Hannan, Shelby A Powers, Vinson M Wang, Fabio Castiglione, Petter Hedlund, Trinity J Bivalacqua
INTRODUCTION AND HYPOTHESIS: Bilateral pelvic nerve injury (BPNI) is a model of post-radical hysterectomy neuropraxia, a common sequela. This study assessed the time course of changes to detrusor autonomic innervation, smooth muscle (SM) content and cholinergic-mediated contraction post-BPNI. METHODS: Female Sprague-Dawley rats underwent BPNI or sham surgery and were evaluated 3, 7, 14, and 30 days post-BPNI (n = 8/group). Electrical field-stimulated (EFS) and carbachol-induced contractions were measured...
December 16, 2016: International Urogynecology Journal
https://www.readbyqxmd.com/read/27960152/effects-of-diabetic-hyperglycemia-on-central-ang-1-7-mas-r-nnos-pathways-in-spontaneously-hypertensive-rats
#15
He Li, Xian Liu, Zhongqiao Ren, Jinxia Gu, Yingjie Lu, Xiaoyun Wang, Lan Zhang
BACKGROUND/AIMS: Hypertension is a major cause of stroke, and diabetes can increase incidence of this disease. We determined the role played by central angiotensin-(1-7) [Ang-(1-7)] pathway in modulating spontaneously hypertension with diabetic hyperglycemia. METHODS: Western Blot analysis and ELISA were used to determine the protein expression of Ang-(1-7) and its signal pathway Mas-R-nNOS in the cerebral cortex and hippocampus of spontaneously hypertensive rats (SHR) and control animals...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27959574/porcine-dorsal-root-ganglia-ovarian-neurons-are-affected-by-long-lasting-testosterone-treatment
#16
B Jana, K Palus, K Meller, J Całka
We studied the effect of testosterone overdose on the number, distribution and chemical coding of ovarian neurons in the dorsal root ganglia (DRGs) in pigs. On day 3 of the estrous cycle, the ovaries of both the control and experimental gilts were injected with retrograde tracer Fast Blue. From day 4 of the estrous cycle to the expected day 20 of the second studied cycle, the experimental gilts were injected with testosterone, while the control gilts received oil. After the completion of the protocol the Th16-L5 DRGs were collected...
December 13, 2016: Physiological Research
https://www.readbyqxmd.com/read/27940344/melatonin-influences-no-nos-pathway-and-reduces-oxidative-and-nitrosative-stress-in-a-model-of-hypoxic-ischemic-brain-damage
#17
Santos Blanco, Raquel Hernández, Gustavo Franchelli, Manuel Miguel Ramos-Álvarez, María Ángeles Peinado
In this work, using a rat model combining ischemia and hypobaric hypoxia (IH), we evaluate the relationships between the antioxidant melatonin and the cerebral nitric oxide/nitric oxide synthase (NO/NOS) system seeking to ascertain whether melatonin exerts its antioxidant protective action by balancing this key pathway, which is highly involved in the cerebral oxidative and nitrosative damage underlying these pathologies. The application of the IH model increases the expression of the three nitric oxide synthase (NOS) isoforms, as well as nitrogen oxide (NOx) levels and nitrotyrosine (n-Tyr) impacts on the cerebral cortex...
January 30, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/27935981/sympathetic-hyperactivity-increased-tyrosine-hydroxylase-and-exaggerated-corpus-cavernosum-relaxations-associated-with-oxidative-stress-plays-a-major-role-in-the-penis-dysfunction-in-townes-sickle-cell-mouse
#18
Fábio H Silva, Mário A Claudino, Fabiano B Calmasini, Eduardo C Alexandre, Carla Franco-Penteado, Arthur L Burnett, Edson Antunes, Fernando F Costa
BACKGROUND: Sickle cell disease patients display priapism that may progress to erectile dysfunction. However, little is known about the pathophysiological alterations of corpus cavernosum in sickle cell disease. OBJECTIVE: Thus, this study aimed to evaluate the functional and molecular alterations of sympathetic machinery and nitric oxide-cyclic guanosine monophosphate signaling pathway in Townes transgenic sickle cell disease mice. METHODS: Concentration-response curves to contractile (phenylephrine) and relaxant agents (acetylcholine and sodium nitroprusside) were obtained in corpus cavernosum strips from sickle and C57BL/6 (control) mice...
2016: PloS One
https://www.readbyqxmd.com/read/27931509/transplantation-of-human-urine-derived-stem-cells-transfected-with-pigment-epithelium-derived-factor-to-protect-erectile-function-in-a-rat-model-of-cavernous-nerve-injury
#19
Qiyun Yang, Xin Chen, Tao Zheng, Dayu Han, Heng Zhang, Yanan Shi, Jun Bian, Xiangzhou Sun, Kai Xia, Xiaoyan Liang, Guihua Liu, Yuanyuan Zhang, Chunhua Deng
The aim of this study was to investigate whether intracavernous injection of urine-derived stem cells (USCs) or USCs genetically modified with pigment epithelium-derived factor (PEDF) could protect the erectile function and cavernous structure in a bilateral cavernous nerve injury-induced erectile dysfunction (CNIED) rat model. USCs were cultured from the urine of six healthy male donors. Seventy-five rats were randomly divided into five groups (n=15 per group): sham, bilateral cavernous nerve (CN) crush injury (BCNI), USC, USCGFP+, and USCGFP/PEDF+ groups...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27930657/the-effects-of-auricular-electro-acupuncture-on-ameliorating-the-dysfunction-of-interstitial-cells-of-cajal-networks-and-nnosmrna-expression-in-antrum-of-stz-induced-diabetic-rats
#20
Huan Chen, Weijian Zhu, Jing Lu, Jinqing Fan, Luning Sun, Xiaoke Feng, Hao Liu, Zhaohui Zhang, Yongqing Wang
BACKGROUD: Interstitial cells of Cajal (ICCs) and nNOS play a crucial role in diabetic gastrointestinal dysmotility(DGD). Our previous study found that electro-acupuncture(EA) on ear point 'stomach' could repair the gastric dysrhythmias in rats induced by rectal distention(RD) after meal. However, little were known about the possible effect of auricular electro-acupuncture (AEA) on diabetic rats. Thus, we designed this study to investigate the effect of AEA on streptozotocin(STZ)-induced diabetic rats...
2016: PloS One
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