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https://www.readbyqxmd.com/read/28435094/diammonium-glycyrrhizinate-alleviates-hepatopulmonary-syndrome-via-restoring-superoxide-dismutase-3-activity-in-rats
#1
Xin Zhao, Jinyu Liu, Shijun Yang, Chen Chen, He Yin, Ting Li, Qiuting Wang, Xiaoya Li, Yulin Lin, Dayong Cai
Hepatopulmonary syndrome (HPS) has a fatal hypoxemia from pulmonary shunts. Superoxide dismutase 3 (SOD3) deficiency involves in this pathogenesis. The purpose of this study was to investigate the underlying mechanisms of diammonium glycyrrhizinate (DG) on HPS via SOD3. Carbon tetrachloride induced HPS rats were treated with captopril or DG for 56 days. Blood gas, pulmonary artery pressures, and histological changes were measured. Molecule dynamics of inducible (iNOS), endothelial (eNOS), neuronal nitric oxide synthase (nNOS) and SOD3 were assessed by immunohistochemistry, quantitative RT-PCR and western blot...
April 20, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28432311/enteric-innervation-combined-with-proteomics-for-the-evaluation-of-the-effects-of-chronic-fluoride-exposure-on-the-duodenum-of-rats
#2
Carina Guimarães de Souza Melo, Juliana Vanessa Colombo Martins Perles, Jacqueline Nelisis Zanoni, Sara Raquel Garcia de Souza, Erika Xavier Santos, Aline de Lima Leite, Alessandro Domingues Heubel, Camila Oliveira E Souza, Juliana Gadelha de Souza, Marília Afonso Rabelo Buzalaf
Ingested fluoride (F) is absorbed mainly in the small intestine, which is controlled by the Enteric Nervous System (ENS). Although important intestinal symptomatology has been described after excessive F exposure, there have been no studies reporting the effects of F on the ENS. In this study, the effects of chronic F exposure were evaluated on the duodenums of rats through proteomic and morphological analyses. Concentrations of 0, 10, or 50 ppm of F were applied to the drinking water for 30 days. Immunofluorescence techniques were performed in the myenteric plexus of the duodenum to detect HuC/D, neuronal nitric oxide (nNOS), vasoactive intestinal peptide (VIP), calcitonin gene related peptide (CGRP), and substance P (SP)...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28430607/simulated-weightlessness-affects-the-expression-and-activity-of-neuronal-nitric-oxide-synthase-in-the-rat-brain
#3
Nara Yoon, Kiyong Na, Hyun-Soo Kim
Spaceflight induces pathophysiological alterations in various organs. To study pathophysiological adaptations to weightlessness on the ground, the tail suspension (TS) rat model has been used to simulate the effects of weightlessness. There is currently little information on the effect of TS on the expression and activity of nitric oxide synthase (NOS) in the brain. In this study, we examined time-dependent alterations in the expression and activity of neuronal NOS (nNOS) in the brains of TS rats. Male Sprague-Dawley rats were tail-suspended for 1 (TS1), 7 (TS7), and 14 (TS14) days or rested on the ground for 3 days after 14 days of TS...
February 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422949/heparin-binding-egf-like-growth-factor-promotes-neuronal-nitric-oxide-synthase-expression-and-protects-the-enteric-nervous-system-after-necrotizing-enterocolitis
#4
Yu Zhou, Yijie Wang, Jacob Olson, Jixin Yang, Gail E Besner
BACKGROUND: Neonatal necrotizing enterocolitis (NEC) is associated with alterations of the ENS, with loss of neuronal nitric oxide synthase (nNOS) expressing neurons in the intestine. The aims of this study were to investigate the roles of heparin binding EGF-like growth factor (HB-EGF) in neural stem cell (NSC) differentiation, nNOS expression, and effects on ENS integrity during experimental NEC. METHODS: The effects of HB-EGF on NSC differentiation and nNOS production were determined using cultured enteric NSC...
April 19, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28422508/nitrile-in-the-hole-discovery-of-a-small-auxiliary-pocket-in-neuronal-nitric-oxide-synthase-leading-to-the-development-of-potent-and-selective-2-aminoquinoline-inhibitors
#5
Maris A Cinelli, Huiying Li, Georges Chreifi, Thomas L Poulos, Richard B Silverman
Neuronal nitric oxide synthase (nNOS) inhibition is a promising strategy to treat neurodegenerative disorders, but the development of nNOS inhibitors is often hindered by poor pharmacokinetics. We previously developed a class of membrane-permeable 2-aminoquinoline inhibitors and later rearranged the scaffold to decrease off-target binding. However, the resulting compounds had decreased permeability, low human nNOS activity, and low selectivity versus human eNOS. In this study, 5-substituted phenyl ether-linked aminoquinolines and derivatives were synthesized and assayed against purified NOS isoforms...
April 19, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28417342/adipose-tissue-derived-stem-cell-therapy-for-erectile-dysfunction-in-rats-a-systematic-review-and-meta-analysis
#6
REVIEW
Quan-Liang Hou, Meng-Ying Ge, Cheng-da Zhang, Dan-Dan Tian, Lian-Ke Wang, Hui-Zi Tian, Wen-Hua Wang, Wei-Dong Zhang
OBJECTIVE: We aimed to systematically assess the effect of adipose tissue-derived stem cell (ADSC) therapy and its influential factors on the treatment of erectile dysfunction (ED) in rats. METHODS: Two authors independently searched for published studies through PubMed and EMBASE from study inception until August 31, 2016. A meta-analysis was used to combine the effect estimate from the published studies. A subgroup analysis was performed to identify the effect of some influential factors...
April 17, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28416970/dna-methylation-profiling-in-peripheral-lung-tissues-of-smokers-and-patients-with-copd
#7
Isaac K Sundar, Qiangzong Yin, Brian S Baier, Li Yan, Witold Mazur, Dongmei Li, Martha Susiarjo, Irfan Rahman
BACKGROUND: Epigenetics changes have been shown to be affected by cigarette smoking. Cigarette smoke (CS)-mediated DNA methylation can potentially affect several cellular and pathophysiological processes, acute exacerbations, and comorbidity in the lungs of patients with chronic obstructive pulmonary disease (COPD). We sought to determine whether genome-wide lung DNA methylation profiles of smokers and patients with COPD were significantly different from non-smokers. We isolated DNA from parenchymal lung tissues of patients including eight lifelong non-smokers, eight current smokers, and eight patients with COPD and analyzed the samples using Illumina's Infinium HumanMethylation450 BeadChip...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28411102/zl006-protects-spinal-cord-neurons-against-ischemia-induced-oxidative-stress-through-ampk-pgc-1%C3%AE-sirt3-pathway
#8
Shu-Guang Liu, Yun-Mei Wang, Yan-Jun Zhang, Xi-Jing He, Tao Ma, Wei Song, Yu-Min Zhang
Spinal cord ischemia (SCI) induces a range of cellular and molecular cascades, including activation of glutamate receptors and downstream signaling. Post-synaptic density protein 95 (PSD-95) links neuronal nitric oxide synthase (nNOS) with the N-methyl-d-aspartic acid (NMDA) receptors to form a ternary complex in the CNS. This molecular complex-mediated cytotoxicity has been implicated in brain ischemia, but its role in SCI has not been determined. The goal of the study was to investigate the potential protective effects of ZL006, a small-molecule inhibitor of the PSD-95/nNOS interaction, in an in vitro SCI model induced by oxygen and glucose deprivation (OGD) in cultured spinal cord neurons...
April 12, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28404583/acute-hypoxia-activates-neuroendocrine-but-not-pre-sympathetic-neurons-in-the-paraventricular-nucleus-of-the-hypothalamus-differential-role-of-nitric-oxide
#9
K Max Coldren, De-Pei Li, David D Kline, Eileen M Hasser, Cheryl M Heesch
Hypoxia results in decreased arterial PO2, arterial chemoreflex activation, and compensatory increases in breathing, sympathetic outflow, and neuroendocrine secretions, including increased secretion of vasopressin (AVP), corticotropin releasing hormone (CRH), adrenocorticotropin hormone (ACTH), and corticosterone. In addition to a brainstem pathway including the nucleus tractus solitarius (nTS) and the rostral ventrolateral medulla (RVLM), medullary pathways to the paraventricular nucleus of the hypothalamus (PVN) contribute to chemoreflex responses...
April 12, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28401316/the-orally-active-noncompetitive-ampar-antagonist-perampanel-attenuates-focal-cerebral-ischemia-injury-in-rats
#10
Hong-Xia Niu, Jun-Zhe Wang, Dong-Liang Wang, Jun-Jie Miao, Hua Li, Zhi-Gang Liu, Xing Yuan, Wei Liu, Jing-Ru Zhou
Inhibition of ionotropic glutamate receptors (iGluRs) is a potential target of therapy for ischemic stroke. Perampanel is a potent noncompetitive α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor (AMPAR) antagonist with good oral bioavailability and favorable pharmacokinetic properties. Here, we investigated the potential protective effects of perampanel against focal cerebral ischemia in a middle cerebral artery occlusion (MCAO) model in rats. Oral administration with perampanel significantly reduced MCAO-induced brain edema, brain infarct volume, and neuronal apoptosis...
April 11, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28396702/interaction-between-prenatal-pesticide-exposure-and-a-common-polymorphism-in-the-pon1-gene-on-dna-methylation-in-genes-associated-with-cardio-metabolic-disease-risk-an-exploratory%C3%A2-study
#11
Ken Declerck, Sylvie Remy, Christine Wohlfahrt-Veje, Katharina M Main, Guy Van Camp, Greet Schoeters, Wim Vanden Berghe, Helle R Andersen
BACKGROUND: Prenatal environmental conditions may influence disease risk in later life. We previously found a gene-environment interaction between the paraoxonase 1 (PON1) Q192R genotype and prenatal pesticide exposure leading to an adverse cardio-metabolic risk profile at school age. However, the molecular mechanisms involved have not yet been resolved. It was hypothesized that epigenetics might be involved. The aim of the present study was therefore to investigate whether DNA methylation patterns in blood cells were related to prenatal pesticide exposure level, PON1 Q192R genotype, and associated metabolic effects observed in the children...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28388365/protective-effects-of-telmisartan-and-tempol-on-lipopolysaccharide-induced-cognitive-impairment-neuro-inflammation-and-amyloidogenesis-possible-role-of-brain-derived-neurotrophic-factor
#12
Waleed Abd-Elsalam Khallaf, Basim Messiha, Amira Abo-Youssef, Nesrine Salah El Dine El Sayed
Angiotensin II has pro-inflammatory and pro-oxidant potentials. We investigated the possible protective effects of the angiotensin receptor blocker telmisartan, compared with the superoxide scavenger tempol, on lipopolysaccharide (LPS)-induced cognitive decline and amyloidogenesis. Briefly, mice were allocated into a normal control group, an LPS control group, a tempol treatment group, and two telmisartan treatment groups. A behavioral study was conducted followed by a biochemical study via assessment of brain levels of beta amyloid (Aβ) and brain-derived neurotropic factor (BDNF) as amyloidogenesis and neuroplasticity markers, tumor necrosis factor-alpha (TNF-α), nitric oxide end products (NOx), neuronal and inducible nitric oxide synthase (nNOS and iNOS) as inflammatory markers, and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione reduced (GSH) and nitrotyrosine (NT) as oxido-nitrosative stress markers...
April 7, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28369791/activation-of-%C3%AE-1a-adrenoceptors-desensitizes-the-rat-aorta-response-to-phenylephrine-through-a-nnos-pathway-a-mechanism-lost-with-aging
#13
Cristina Arce, Diana Vicente, Vanessa Segura, Nicla Flacco, Fermi Montó, Luis Almenar, Jaime Agüero, Joaquín Rueda, Francesc Jiménez-Altayó, Elisabet Vila, Maria Antonia Noguera, Pilar D'Ocon, Maria Dolores Ivorra
BACKGROUND AND PURPOSE: A NO-mediated desensitization of α1 -adrenoceptor (AR) vasoconstrictor response has been reported in different vessels. We investigated the involvement of each α1 -AR subtype and constitutive NOS isoforms and the influence of aging and hypertension in this process. EXPERIMENTAL APPROACH: Wistar and spontaneously hypertensive rats (SHR) of 16, 32, 52 and 72-week-old were used to evaluate the desensitization process. Expression of α1 -AR subtypes, eNOS and nNOS in the rat aorta and left ventricle (LV) were determined...
March 29, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28360833/efficient-binding-of-the-nos1ap-c-terminus-to-the-nnos-pdz-pocket-requires-the-concerted-action-of-the-pdz-ligand-motif-the-internal-exf-site-and-structural-integrity-of-an-independent-element
#14
Li-Li Li, Katryna Cisek, Michael J Courtney
Neuronal nitric oxide synthase is widely regarded as an important contributor to a number of disorders of excitable tissues. Recently the adaptor protein NOS1AP has emerged as a contributor to several nNOS-linked conditions. As a consequence, the unexpectedly complex mechanisms of interaction between nNOS and its effector NOS1AP have become a particularly interesting topic from the point of view of both basic research and the potential for therapeutic applications. Here we demonstrate that the concerted action of two previously described motif regions contributing to the interaction of nNOS with NOS1AP, the ExF region and the PDZ ligand motif, efficiently excludes an alternate ligand from the nNOS-PDZ ligand-binding pocket...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28360194/neuronal-nitric-oxide-synthase-derived-hydrogen-peroxide-effect-in-grafts-used-in-human-coronary-bypass-surgery
#15
Patrick Wander Endlich, Rosária Dias Aires, Roberta Lins Gonçalves, Eduardo Damasceno Costa, Janaína de Paula Arantes Ângelo, Lucas Ferreira Alves, Rafaela Fernandes da Silva, Bruno Almeida Rezende, Steyner F Cortes, Virgínia S Lemos
Recently, H2O2 has been identified as the endothelium-dependent hyperpolarizing factor (EDHF), which mediates flow-induced dilation in human coronary arteries. Neuronal nitric oxide synthase (nNOS) is expressed in the cardiovascular system and besides NO, generates H2O2 The role of nNOS-derived H2O2 in human vessels is so far unknown. The present study aimed at investigating the relevance of nNOS/H2O2 signaling in human internal mammary artery (IMA) and saphenous vein (SV), the major conduits used in coronary artery bypass grafting...
March 30, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28358321/the-influence-of-tetrodotoxin-ttx-on-the-distribution-and-chemical-coding-of-caudal-mesenteric-ganglion-camg-neurons-supplying-the-porcine-urinary-bladder
#16
Ewa Lepiarczyk, Agnieszka Bossowska, Jerzy Kaleczyc, Marta Majewska, Sławomir Gonkowski, Mariusz Majewski
The treatment of micturition disorders creates a serious problem for urologists. Recently, new therapeutic agents, such as neurotoxins, are being considered for the therapy of urological patients. The present study investigated the chemical coding of caudal mesenteric ganglion (CaMG) neurons supplying the porcine urinary bladder after intravesical instillation of tetrodotoxin (TTX). The CaMG neurons were visualized with retrograde tracer Fast blue (FB) and their chemical profile was disclosed with double-labeling immunohistochemistry using antibodies against tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), calbindin (CB), galanin (GAL) and neuronal nitric oxide synthase (nNOS)...
March 30, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28338308/penile-alterations-at-early-stage-of-type-1-diabetes-in-rats
#17
Mingfang Tao, Cemal Tasdemir, Seda Tasdemir, Ali Shahabi, Guiming Liu
OBJECTIVE: Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats. MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues...
March 24, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28326018/neuronal-nitric-oxide-synthase-in-neural-stem-cells-induces-neuronal-fate-commitment-via-the-inhibition-of-histone-deacetylase-2
#18
Xing Jin, Zhang-Feng Yu, Fang Chen, Guang-Xian Lu, Xin-Yuan Ding, Lin-Jun Xie, Jian-Tong Sun
Active adult neurogenesis produces new functional neurons, which replace the lost ones and contribute to brain repair. Promoting neurogenesis may offer a therapeutic strategy for human diseases associated with neurodegeneration. Here, we report that endogenous neuronal nitric oxide synthase (nNOS) for neural stem cells (NSCs) or progenitors positively regulates neurogenesis. nNOS repression exhibits significantly decreased neuronal differentiation and nNOS upregulation promotes neurons production from NSCs...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28324630/morphological-and-neurochemical-differences-in-peptidergic-nerve-fibers-of-the-mouse-vagina
#19
Christine M Barry, Esther Ji, Harman Sharma, Lara Beukes, Patricia I Vilimas, Yvette C DeGraaf, Dusan Matusica, Rainer V Haberberger
The vagina is innervated by a complex arrangement of sensory, sympathetic, and parasympathetic nerve fibers that contain classical transmitters plus an array of neuropeptides and enzymes known to regulate diverse processes including blood flow and nociception. The neurochemical characteristics and distributions of peptide-containing nerves in the mouse vagina are unknown. This study used multiple labeling immunohistochemistry, confocal maging and analysis to investigate the presence and colocalization of the peptides vasoactive intestinal polypeptide (VIP), calcitonin-gene related peptide (CGRP), substance P (SP), neuropeptide tyrosine (NPY), and the nitric oxide synthesizing enzyme neuronal nitric oxide synthase (nNOS) in nerve fibers of the murine vaginal wall...
March 21, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28322084/fine-structure-and-synaptology-of-the-nitrergic-neurons-in-medial-septum-of-the-rat-brain
#20
Katalin Halasy, Balázs Szőke, Gergely Janzsó
The nitrergic neuron population and certain aspects of their connectivity (peptidergic inputs, co-localization with GABA, synaptic target distribution) were studied in the medial septum of the rat brain. The histochemical localization of NADPH diaphorase and immunohistochemical identification of nNOS at light and electron microscopic level was applied. Double-labeling experiments with galanin and leucine enkephalin, moreover the postembedding GABA immunogold staining was also carried out. NADPH diaphorase- and nNOS-immunopositive neurons could be identified inside the borders of medial septum...
March 2017: Acta Biologica Hungarica
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