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https://www.readbyqxmd.com/read/29035821/neuronal-nitric-oxide-synthase-is-involved-in-cb-trpv1-signalling-focus-on-control-of-hippocampal-hyperexcitability
#1
Fabio Carletti, Giuditta Gambino, Valerio Rizzo, Giuseppe Ferraro, Pierangelo Sardo
Cannabinoids (CB), transient receptors potential vanilloid type 1 (TRPV1) and nitric oxide (NO) were found to be interlinked in regulating some neuronal functions such as membrane excitability and synaptic transmission. TRPV1 play a fundamental role since it represents a synaptic target for CB that triggers several downstream cellular pathways. In this regard, recent evidence report that TRPV1 could influence NO production by modulating neuronal NO synthase (nNOS) activity. In the present research, we pointed to manipulate nNOS function to assess its role on TRPV1 signalling in hyperexcitability conditions elicited in the dentate gyrus of hippocampal formation...
October 3, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/29021742/neuroprotective-mechanisms-of-lycium-barbarum-polysaccharides-against-ischemic-insults-by-regulating-nr2b-and-nr2a-containing-nmda-receptor-signaling-pathways
#2
Zhongshan Shi, Lihui Zhu, Tingting Li, Xiaoya Tang, Yonghui Xiang, Xinjia Han, Luoxing Xia, Ling Zeng, Junhua Nie, Yongxia Huang, Chi Kwan Tsang, Ying Wang, Zhigang Lei, Zaocheng Xu, Kwok-Fai So, Yiwen Ruan
Glutamate excitotoxicity plays an important role in neuronal death after ischemia. However, all clinical trials using glutamate receptor inhibitors have failed. This may be related to the evidence that activation of different subunit of NMDA receptor will induce different effects. Many studies have shown that activation of the intrasynaptic NR2A subunit will stimulate survival signaling pathways, whereas upregulation of extrasynaptic NR2B will trigger apoptotic pathways. A Lycium barbarum polysaccharide (LBP) is a mixed compound extracted from Lycium barbarum fruit...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29016640/effects-of-aav-mediated-knockdown-of-nnos-and-gpx-1-gene-expression-in-rat-hippocampus-after-traumatic-brain-injury
#3
Deborah R Boone, Jeanna M Leek, Michael T Falduto, Karen E O Torres, Stacy L Sell, Margaret A Parsley, Jeremy C Cowart, Tatsuo Uchida, Maria-Adelaide Micci, Douglas S DeWitt, Donald S Prough, Helen L Hellmich
Virally mediated RNA interference (RNAi) to knock down injury-induced genes could improve functional outcome after traumatic brain injury (TBI); however, little is known about the consequences of gene knockdown on downstream cell signaling pathways and how RNAi influences neurodegeneration and behavior. Here, we assessed the effects of adeno-associated virus (AAV) siRNA vectors that target two genes with opposing roles in TBI pathogenesis: the allegedly detrimental neuronal nitric oxide synthase (nNOS) and the potentially protective glutathione peroxidase 1 (GPx-1)...
2017: PloS One
https://www.readbyqxmd.com/read/28982169/the-effect-of-cavernous-nerve-traction-on-erectile-function-in-rats
#4
Hao Li, Liping Chen, Tao Wang, Shaogang Wang, Jihong Liu
We performed this study to evaluate the effect of cavernous nerve (CN) traction on erectile function in rats. Thirty-two 8- week-old Sprague-Dawley rats were divided into four groups: control, 1-minute CN traction, 2-minute CN traction, and 2-minute CN crush. CN traction was performed using a glass hook with a tensile force of 0.2 Newton. One month later, the mean arterial pressure (MAP) and intracavernosal pressure (ICP) in response to CN stimulation were measured to assess erectile function. The penis and major pelvic ganglion (MPG) were harvested to explore the expression of neuronal nitric oxide synthase (nNOS) and neurofilament, fibrosis and apoptosis...
2017: PloS One
https://www.readbyqxmd.com/read/28975622/the-low-affinity-neurotensin-receptor-antagonist-levocabastine-impairs-brain-nitric-oxide-synthesis-and-mitochondrial-function-by-independent-mechanisms
#5
Silvia Lores-Arnaiz, Analía G Karadayian, Alicia Gutnisky, Georgina Rodríguez de Lores Arnaiz
Neurotensin is known to inhibit neuronal Na+, K+ -ATPase, an effect that is rescued by nitric oxide (NO) synthase inhibition. However, whether the neurotensinergic and the nitrergic systems are independent pathways, or are mechanistically linked, is unknown. Here, we addressed this issue and found that the administration of NTS2 antagonist, levocabastine (50 μg/kg, i.p.) inhibited NO synthase (NOS) activity by 74 and 42% after 18 h in synaptosomal and mitochondrial fractions isolated from the Wistar rat cerebral cortex, respectively; these effects disappeared 36 h after levocabastine treatment...
October 4, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28974418/mge-derived-nnos-interneurons-promote-fear-acquisition-in-nnos-mice
#6
Lin Zhang, Hong-Jin Yuan, Bo Cao, Cheng-Cheng Kong, Fang Yuan, Jun Li, Huan-Yu Ni, Hai-Yin Wu, Lei Chang, Yan Liu, Chun-Xia Luo
Neuronal nitric oxide synthase (nNOS) (1), mainly responsible for NO release in central nervous system (CNS) (2), plays a significant role in multiple physiological functions. However, the function of nNOS(+) interneurons in fear learning has not been much explored. Here we focused on the medial ganglionic eminences (MGE) (3)-derived nNOS(+) interneurons in fear learning. To determine the origin of nNOS(+) interneurons, we cultured neurons in vitro from MGE, cortex, lateral ganglionic eminence (LGE) (4), caudal ganglionic eminences (CGE) (5) and preoptic area (POA) (6)...
September 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28972567/the-influence-of-resiniferatoxin-rtx-and-tetrodotoxin-ttx-on-the-distribution-relative-frequency-and-chemical-coding-of-noradrenergic-and-cholinergic-nerve-fibers-supplying-the-porcine-urinary-bladder-wall
#7
Ewa Lepiarczyk, Agnieszka Bossowska, Jerzy Kaleczyc, Agnieszka Skowrońska, Marta Majewska, Michal Majewski, Mariusz Majewski
The present study investigated the influence of intravesically instilled resiniferatoxin (RTX) or tetrodotoxin (TTX) on the distribution, number, and chemical coding of noradrenergic and cholinergic nerve fibers (NF) supplying the urinary bladder in female pigs. Samples from the bladder wall were processed for double-labelling immunofluorescence with antibodies against cholinergic and noradrenergic markers and some other neurotransmitter substances. Both RTX and TTX caused a significant decrease in the number of cholinergic NF in the urinary bladder wall (in the muscle coat, submucosa, and beneath the urothelium)...
October 3, 2017: Toxins
https://www.readbyqxmd.com/read/28970724/changes-in-expression-of-inhibitory-substances-in-the-intramural-neurons-of-the-stomach-following-streptozotocin-induced-diabetes-in-the-pig
#8
Michał Bulc, Katarzyna Palus, Łukasz Zielonka, Magdalena Gajęcka, Jarosław Całka
AIM: Influence of chronic hyperglycemia on chemical coding of enteric neurons in stomach using pig as a model for human diabetic complications. METHODS: Ten pigs were divided into two groups: diabetic (D group, n = 5) and control (C group, n = 5). Pigs constituting the experimental group were given streptozotocin (150 mg/kg). Animals were euthanized six weeks after the induction of diabetes. The samples of stomach were collected from animals of both groups. The cryostat sections were processed for double immunofluorescence staining using primary antisera directed towards pan-neuronal marker (Hu C/D) proteins and/or neuronal isoform of nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP) and galanin (GAL)...
September 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28970060/nnos-uncoupling-by-oxidized-ldl-implications-in-atherosclerosis
#9
Juliana Maria Navia-Pelaez, Gianne Paul Campos-Mota, Jessica Cristina Araujo de Souza, Edenil Costa Aguilar, Nikos Stergiopulos, Jaqueline Izaura Alvarez-Leite, Luciano Santos Aggum Capettini
No abstract text is available yet for this article.
September 29, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28969107/intracarotid-sodium-nitroprusside-on-fifth-post-ischemic-stroke-day-in-middle-cerebral-artery-occlusion-rat-model
#10
Vinod Kumar Tewari, Vivek Bhosale, Rakesh Shukla, Hari Kishan Das Gupta, Sheeba
INTRODUCTION: Ischemic stroke at later stages (>4.5 hour) have very few treatment options left. In those cases Nitric Oxide (NO) may provide promising results. NO is active in signaling pathways. Sodium Nitroprusside (SNP), a NO donor was tested earlier in rat Middle Cerebral Artery Occlusion (MCAO) model in early stages (5-60 minutes) and found useful but in delayed stroke cases (60-120 minutes) found useless. This was due to local inducible Nitric Oxide Synthase Enzyme (iNOS) and superoxide (causes destructive effect) formation which was skipped...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28941690/does-l-glutamine-supplemented-diet-extenuate-no-mediated-damage-on-myenteric-plexus-of-walker-256-tumor-bearing-rats
#11
Geraldo Emílio Vicentini, Heber Amilcar Martins, Luciane Fracaro, Sara Raquel Garcia de Souza, Kassio Papi da Silva Zanoni, Thamara Nishida Xavier Silva, Fernanda Paschoal Blegniski, Flávia Alessandra Guarnier, Jacqueline Nelisis Zanoni
This study was designed to appraise the relationship between enteric neuropathy and oxidative stress in cancer cachexia under l-glutamine-supplemented diet. Total and nitrergic neuronal populations were investigated in jejunum and ileum in four experimental groups: control (C); control l-glutamine-supplemented diet (CG); Walker-256 tumor (TW); and Walker-256 tumor supplemented with l-glutamine (TWG). In addition, local oxidative stress, neuronal nitric oxide synthase (nNOS) enzyme and nitric oxide (NO) levels were evaluated...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28933095/-investigation-of-effects-of-albiflorin-and-paeoniflorin-on-hippocampal-bdnf-and-no-in-chronic-restraint-stress-rats
#12
Ying-Li Zhu, Lin-Yuan Wang, Cheng-Long Wang, Dan-Ping Zhao, Sha Wang, Wen-Ting Fei, Jian-Jun Zhang
This paper aimed to investigate the antidepressant-like effects and the mechanism of the albiflorin, paeoniflorin on rats with chronic restraint stress model. Fluoxetine and Xiaoyao group served as the positive control, body weight, sucrose preference test and the open field behavioral experiment were measured, the levels of nitric oxide (NO) in hippocampus were detected by Greg colorimetric method. Furthermore, the levels of brain derived neurotrophic factor (BDNF) in hippocampus were detected by ELISA. Finally, the expressions of nNOS mRNA in hippocampus detected by RT-qPCR, the protein levels of nNOS and BDNF in hippocampus were detected by Western blot...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28932757/a-five-repeat-micro-dystrophin-gene-ameliorated-dystrophic-phenotype-in-the-severe-dba-2j-mdx-model-of-duchenne-muscular-dystrophy
#13
Chady H Hakim, Nalinda B Wasala, Xiufang Pan, Kasun Kodippili, Yongping Yue, Keqing Zhang, Gang Yao, Brittney Haffner, Sean X Duan, Julian Ramos, Joel S Schneider, N Nora Yang, Jeffrey S Chamberlain, Dongsheng Duan
Micro-dystrophins are highly promising candidates for treating Duchenne muscular dystrophy, a lethal muscle disease caused by dystrophin deficiency. Here, we report robust disease rescue in the severe DBA/2J-mdx model with a neuronal nitric oxide synthase (nNOS)-binding micro-dystrophin vector. 2 × 10(13) vector genome particles/mouse of the vector were delivered intravenously to 10-week-old mice and were evaluated at 6 months of age. Saturated micro-dystrophin expression was detected in all skeletal muscles and the heart and restored the dystrophin-associated glycoprotein complex and nNOS...
September 15, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28932186/da-negatively-regulates-igf-i-actions-implicated-in-cognitive-function-via-interaction-of-psd95-and-nnos-in-minimal-hepatic-encephalopathy
#14
Saidan Ding, Weishan Zhuge, Xuebao Wang, Jianjing Yang, Yuanshao Lin, Chengde Wang, Jiangnan Hu, Qichuan Zhuge
Insulin-like growth factor I (IGF-I) has been positively correlated with cognitive ability. Cognitive decline in minimal hepatic encephalopathy (MHE) was shown to be induced by elevated intracranial dopamine (DA). The beneficial effect of IGF-I signaling in MHE remains unknown. In this study, we found that IGF-I content was reduced in MHE rats and that IGF-I administration mitigated cognitive decline of MHE rats. A protective effect of IGF-I on the DA-induced interaction between postsynaptic density protein 95 (PSD95) and neuronal nitric oxide synthase (nNOS) was found in neurons...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28921679/maternal-immune-activation-leads-to-increased-nnos-immunoreactivity-in-the-brain-of-postnatal-day-2-rat-offspring
#15
Jiaxian Zhang, Yu Jing, Hu Zhang, David K Bilkey, Ping Liu
Neuronal nitric oxide synthase (nNOS) is a key arginine metabolising enzyme in the brain, and nNOS-derived nitric oxide plays an important role in regulating glutamatergic neurotransmission. Nitric oxide and its related molecules are involved in the pathogenesis of schizophrenia, and human genetic studies have identified schizophrenia risk genes encoding nNOS. The present study systematically investigated how maternal immune activation (MIA; a risk factor for schizophrenia) induced by polyinosinic:polycytidylic acid affected nNOS-immunoreactivity in the brain of the resulting male and female offspring at the age of postnatal day (PND) 2...
September 16, 2017: Synapse
https://www.readbyqxmd.com/read/28901462/role-of-ghrelin-in-small-intestinal-motility-following-pediatric-intracerebral-hemorrhage-in-mice
#16
Jieyu Zan, Lei Song, Jiejie Wang, Rong Zou, Fei Hong, Jinhua Zhao, Yijun Cheng, Ming Xu
Small intestinal motility (SIM) disorder is a common complication following pediatric intracerebral hemorrhage (ICH), leading to a poor prognosis in patients. Previous studies have shown that ghrelin is involved in SIM in various diseases; however, the role of ghrelin in pediatric ICH‑induced SIM disorder remains to be elucidated. The present study was designed to investigate the association between ghrelin and SIM post‑ICH, and to examine the effect of exogenous ghrelin administration on SIM in vivo...
September 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28893594/nitric-oxide-in-the-medial-prefrontal-cortex-contributes-to-the-acquisition-of-cocaine-place-preference-and-synaptic-plasticity-in-the-laterodorsal-tegmental-nucleus
#17
Hironori Kamii, Naofumi Taoka, Masabumi Minami, Katsuyuki Kaneda
Nitric oxide (NO), a gaseous neurotransmitter, is involved in a variety of brain functions, including drug addiction. Although previous studies have suggested that NO plays an important role in the development of cocaine addiction, the brain region(s) in which NO acts and how it contributes to cocaine addiction remain unclear. In this study, we examined these issues using a cocaine-induced conditioned place preference (CPP) paradigm and ex vivo electrophysiological recordings in rats. Specifically, we focused on the medial prefrontal cortex (mPFC) and laterodorsal tegmental nucleus (LDT), brain regions associated with cocaine CPP development and cocaine-induced plasticity...
September 8, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28893245/age-dependent-redox-status-in-the-brain-stem-of-no-deficient-hypertensive-rats
#18
Miroslava Majzúnová, Zuzana Pakanová, Peter Kvasnička, Peter Bališ, Soňa Čačányiová, Ima Dovinová
BACKGROUND: The brain stem contains important nuclei that control cardiovascular function via the sympathetic nervous system (SNS), which is strongly influenced by nitric oxide. Its biological activity is also largely determined by oxygen free radicals. Despite many experimental studies, the role of AT1R-NAD(P)H oxidase-superoxide pathway in NO-deficiency is not yet sufficiently clarified. We determined changes in free radical signaling and antioxidant and detoxification response in the brain stem of young and adult Wistar rats during chronic administration of exogenous NO inhibitors...
September 11, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28888982/disrupting-nnos-psd-95-coupling-in-the-hippocampal-dentate-gyrus-promotes-extinction-memory-retrieval
#19
Jun Li, Zhou Han, Bo Cao, Cheng-Yun Cai, Yu-Hui Lin, Fei Li, Hai-Ying Wu, Lei Chang, Chun-Xia Luo, Dong-Ya Zhu
Granule cells in the dentate gyrus regenerate constantly in adult hippocampus and then integrate into neural circuits in the hippocampus thereby providing the neural basis for learning and memory. Promoting the neurogenesis in the hippocampus facilitates learning and memory such as spatial learning, object identification, and extinction learning. The interaction between neuronal nitric oxide synthase (nNOS) and postsynaptic density protein-95 (PSD-95) is reported to negatively regulate neurogenesis in brain, so we hypothesized that disrupting this interaction might facilitate the neurogenesis in the dentate gyrus (DG) and thus enhance the extinction memory retrieval of fear learning...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28887183/electrical-stimulation-of-the-hippocampal-fimbria-facilitates-neuronal-nitric-oxide-synthase-activity-in-the-medial-shell-of-the-rat-nucleus-accumbens-modulation-by-dopamine-d1-and-d2-receptor-activation
#20
Kristina E Hoque, Shannon R Blume, Stephen Sammut, Anthony R West
The medial shell region of the nucleus accumbens (msNAc) is a key center for the regulation of goal-directed behavior and is likely to be dysfunctional in neuropsychiatric disorders such as addiction, depression and schizophrenia. Nitric oxide (NO)-producing interneurons in the msNAc are potently modulated by dopamine (DA) and may play an important role in synaptic integration in msNAc networks. In this study, neuronal NO synthase (nNOS) activity was measured in anesthetized rats using amperometric microsensors implanted into the msNAc or via histochemical techniques...
November 2017: Neuropharmacology
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