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https://www.readbyqxmd.com/read/29457744/optimal-safe-implantation-corridors-in-feline-cervical-vertebrae-c2-t1-ct-study-in-16-domestic-shorthair-cats
#1
Irene Espadas, Thomas W Maddox, Felipe de Vicente
Objectives The objective of this study was to define safe corridors for the optimal placement of bicortical implants in the feline cervical spine (C2-T1) using CT. Methods CT images of feline cervical spines (n = 16) were reviewed retrospectively. Multiplanar reconstructions were used to define the optimal safe corridors. Safe corridors were defined by their angle of insertion, width and length. The insertion point within the vertebral body was also described. Vertebral measurements were compared between vertebrae using multilevel linear regression, and left and right measurements within vertebrae were compared with the paired samples Wilcoxon signed-rank test...
February 1, 2018: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/29428005/effects-of-branched-chain-volatile-fatty-acids-on-lactation-performance-and-mrna-expression-of-genes-related-to-fatty-acid-synthesis-in-mammary-gland-of-dairy-cows
#2
Q Liu, C Wang, G Guo, W J Huo, S L Zhang, C X Pei, Y L Zhang, H Wang
Branched-chain volatile fatty acids (BCVFA) supplements could promote lactation performance and milk quality by improving ruminal fermentation and milk fatty acid synthesis. This study was conducted to evaluate the effects of BCVFA supplementation on milk performance, ruminal fermentation, nutrient digestibility and mRNA expression of genes related to fatty acid synthesis in mammary gland of dairy cows. A total of 36 multiparous Chinese Holstein cows averaging 606±4.7 kg of BW, 65±5.2 day in milk (DIM) with daily milk production of 30...
February 12, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29422105/normal-radiological-anatomy-of-thyroid-cartilage-in-600-chinese-individuals-implications-for-anterior-cervical-spine-surgery
#3
Ying-Zhao Yan, Chong-An Huang, Qi Jiang, Yi Yang, Jian Lin, Ke Wang, Xiao-Bin Li, Hai-Hua Zheng, Xiang-Yang Wang
BACKGROUND: Thyroid cartilage is an important barrier in anterior cervical approach surgery. The objective of this study is to establish normative values for thyroid cartilage at three planes and to determine their significance on preoperative positioning and intraoperative traction in surgery via the anterior cervical approach. METHODS: Neck CT scans were collected from 600 healthy adults who did not meet any of the exclusion criteria. Transverse diameters (D1, D2, and D3) of the superior border of the thyroid cartilage (SBTC), inferior border of the thyroid cartilage (IBTC), and the trachea transverse diameters of the inferior border of the cricoid cartilage (IBCC) were measured on a horizontal plane...
February 8, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29411117/computer-tomography-based-morphometric-analysis-of-the-cervical-spine-pedicles-c3-c7
#4
Leonard Westermann, Carolin Spemes, Peer Eysel, Marvin Simons, Max J Scheyerer, Jan Siewe, Dominik Baschera
BACKGROUND: Our aim was to examine the specific dimensions of cervical pedicles in a large Caucasian cohort on high dissolving CT scans. METHODS: A retrospective analysis of 100 cervical spine CT scans with a maximum slice thickness of 1 mm in axial, sagittal, and coronal reconstructions was performed. The pedicle axial length (PAL), inner and outer pedicle diameter (IPD/OPD), pedicle sagittal and transverse angle (PSA/PTA), pedicle height (PH), pedicle width (PW), and the cortical thickness (COT) at different margins were measured by two independent observers...
February 6, 2018: Acta Neurochirurgica
https://www.readbyqxmd.com/read/29410577/non-surgical-improvement-of-cervical-lordosis-is-possible-in-advanced-spinal-osteoarthritis-a-cbp%C3%A2-case-report
#5
Miles O Fortner, Paul A Oakley, Deed E Harrison
[Purpose] To present a case of the non-surgical improvement in cervical kyphosis in a patient with history of cervical spine trauma and advanced osteoarthritis. [Subject and Methods] A 38 year old male presented with a chief complaint of chronic neck pain that was not substantially relieved by recent previous traditional physiotherapy and chiropractic manipulation. The cervical radiograph demonstrated a cervical hypolordosis of 5° as measured by the Harrison posterior tangent method from C2-C7. There was a 15° kyphosis at C4-C6 with advanced degenerative changes consistent with previous spine trauma...
January 2018: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/29409678/isolation-and-genomic-characterization-of-culex-flaviviruses-from-mosquitoes-in-myanmar
#6
Aung Kyaw Kyaw, Mya Myat Ngwe Tun, Corazon C Buerano, Takeshi Nabeshima, Miako Sakaguchi, Tsuyoshi Ando, Shingo Inoue, Yi Yi Mya, Daisuke Hayasaka, Hlaing Myat Thu, Kyaw Zin Thant, Kouichi Morita
An entomological surveillance of arboviruses was conducted in Myanmar in 2014. A total of 8357 Culex mosquito vectors were collected in the Mandalay area and virus isolation was done by using the mosquito cell line C6/36 E2. A total of eighteen strains of Culex flavivirus (CxFV) were isolated from Cx. tritaeniorhynchus, Cx. vishnui and Cx. fuscocephala. Like other insect-specific flaviviruses, CxFV can replicate only in mosquito cells but not in mammalian cells. These CxFV strains that were isolated in Japan from mosquitoes collected in Myanmar were closely related to the Wang Thong virus detected from Cx fusocephalus in Thailand and Cx...
February 3, 2018: Virus Research
https://www.readbyqxmd.com/read/29392497/inhibition-of-dengue-virus-infection-by-small-interfering-rnas-that-target-highly-conserved-sequences-in-the-ns4b-or-ns5-coding-regions
#7
Paula M Villegas, Elizabeth Ortega, Lourdes Villa-Tanaca, Blanca L Barrón, Jesus Torres-Flores
Dengue fever is one of the most common viral infections in the world. Although a vaccine against dengue virus (DENV) has been approved in several countries, this disease is still considered a public health priority worldwide. The ability of three small interfering RNAs (FG-siRNAs) targeting conserved sequences in the NS4B and NS5 regions of the DENV genome to inhibit DENV replication was tested in vitro in both Vero and C6/36 cells. The FG-siRNAs were effective against DENV-1, -3, and -4, but not DENV-2. A fourth siRNA specifically targeting the NS5 region of the DENV-2 genome (SG-siRNA) was designed and tested against two different DENV-2 strains, showing high levels of inhibition in both mammalian and insect cells...
February 1, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29371358/whole-genome-sequences-of-zika-virus-flr-strains-after-passage-in-vero-or-c6-36-cells
#8
Lindsey A Moser, Lauren M Oldfield, Nadia Fedorova, Vinita Puri, Susmita Shrivastava, Paolo Amedeo, Richard Isom, Lihui Hu, Alan Durbin, Iara Rocchi, Torrey Williams, Gene S Tan, Reed S Shabman, Kristen A Bernard, Brett E Pickett
We report 26 complete genomes of Zika virus (ZIKV) isolated after passaging the Zika virus strain FLR in mosquito (C6/36) and mammalian (Vero) cell lines. The consensus ZIKV genomes we recovered show greater than 99% nucleotide identify with each other and with the FLR strain used as input.
January 25, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29362936/arginine-%C3%AE-%C3%AE-dehydrophenylalanine-dipeptide-nanoparticles-for-ph-responsive-drug-delivery
#9
Pankaj Kumar Singh, Sonika Chibh, Taru Dube, Virander Singh Chauhan, Jiban Jyoti Panda
PURPOSE: Nanoparticles (NPs) exhibiting responsiveness towards pH variations in organs, tissue microenvironments and cellular compartments can significantly add on to the drug delivery potential. Here, we have developed NPs from an amphipathic dipeptide, Arginine-α, β-dehydrophenylalanine (RΔF), and tried to explore their pH responsive drug delivery potential in various cancer cells. METHODS: RΔF-NPs were architectured by harnessing the process of molecular self-assembly followed by the assessment of effect of pH on NPs morphology using zetasizer, SEM and CD...
January 16, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29329394/analysis-of-the%C3%A2-aedes-albopictus%C3%A2-c6-36-genome-provides-insight-into-cell-line-utility-for-viral-propagation
#10
Jason R Miller, Sergey Koren, Kari A Dilley, Vinita Puri, David M Brown, Derek M Harkins, Françoise Thibaud-Nissen, Benjamin Rosen, Xiao-Guang Chen, Zhijian Tu, Igor V Sharakhov, Maria V Sharakhova, Robert Sebra, Timothy B Stockwell, Nicholas H Bergman, Granger G Sutton, Adam M Phillippy, Peter M Piermarini, Reed S Shabman
Background: The 50-year old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, and chikungunya. The cell line is derived from an unknown number of larvae from an unspecified strain of Aedes albopictus mosquitoes. Toward improved utility of the cell line for research in virus transmission, we present an annotated assembly of the C6/36 genome. Results: The C6/36 genome assembly has the largest contig N50 (3...
January 10, 2018: GigaScience
https://www.readbyqxmd.com/read/29274055/impact-of-crossbreeding-holstein-and-brown-swiss-cows-on-milk-yield-composition-and-fatty-acid-profiles-in-subtropics
#11
Mahmoud S El-Tarabany, Akram A El-Tarabany, Sana S Emara
Milk yield (MY), composition, and fatty acid profiles of purebred Holstein (HO) cows, Brown Swiss (BS), and their F1 crossbreds (HS) were compared under subtropical climate in Egypt. Pure HO had significantly greater 305-MY, total-MY, and daily-MY (p < 0.05) than pure BS and crossbred HS. Furthermore, HO and HS had significantly higher peak-MY (44.2 and 43.3 kg, respectively) than BS (36.1 kg). In comparison with HO, BS had significantly higher milk fat, protein, total solids, and solid-not-fat % (p < 0...
December 22, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29258204/zika-virus-exhibits-lineage-specific-phenotypes-in-cell-culture-in-aedes-aegypti-mosquitoes-and-in-an-embryo-model
#12
Katherine A Willard, Leah Demakovsky, Blanka Tesla, Forrest T Goodfellow, Steven L Stice, Courtney C Murdock, Melinda A Brindley
Zika virus (ZIKV) has quietly circulated in Africa and Southeast Asia for the past 65 years. However, the recent ZIKV epidemic in the Americas propelled this mosquito-borne virus to the forefront of flavivirus research. Based on historical evidence, ZIKV infections in Africa were sporadic and caused mild symptoms such as fever, skin rash, and general malaise. In contrast, recent Asian-lineage ZIKV infections in the Pacific Islands and the Americas are linked to birth defects and neurological disorders. The aim of this study is to compare replication, pathogenicity, and transmission efficiency of two historic and two contemporary ZIKV isolates in cell culture, the mosquito host, and an embryo model to determine if genetic variation between the African and Asian lineages results in phenotypic differences...
December 16, 2017: Viruses
https://www.readbyqxmd.com/read/29143280/genome-sequencing-and-phylogenetic-analysis-of-banna-virus-genus-seadornavirus-family-reoviridae-isolated-from-culicoides
#13
Song Song, Yuanyuan Li, Shihong Fu, Wenwen Lei, Xiaofang Guo, Yun Feng, Xiaoyan Gao, Xiaolong Li, Zhonghua Yang, Ziqian Xu, Ying He, Huanyu Wang, Hongning Zhou, Bin Wang, Xiaoqing Lu, Guodong Liang
In an investigation of blood-sucking insects and arboviruses, a virus (YN12243) was isolated from Culicoides samples collected in the Sino-Burmese border region of Yunnan Province, China. The virus caused cytopathic effect (CPE) in C6/36 cells and passaged stably. Polyacrylamide gel analysis showed that the genome of YN12243 was composed of 12 segments of double-stranded RNA (dsRNA), with a distribution pattern of 6-6. The nucleotide and amino acid sequences of the coding region (1‒12 segments) were 17,803 bp and 5,925 amino acids in length, respectively...
October 26, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/29133118/functional-characterization-of-a-serine-protease-inhibitor-modulated-in-the-infection-of-the-aedes-aegypti-with-dengue-virus
#14
Tatiane Sanches Soares, Boris Luis Rodriguez Gonzalez, Ricardo José Soares Torquato, Francisco Jose Alves Lemos, André L Costa-da-Silva, Margareth de Lara Capurro Guimarães, Aparecida Sadae Tanaka
During feeding with blood meal, female Aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and Zika viruses. Dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. In the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. However, little information is available about these molecules in the Ae...
November 10, 2017: Biochimie
https://www.readbyqxmd.com/read/29114369/bimelic-symmetric-hirayama-disease-spectrum-of-magnetic-resonance-imaging-findings-and-comparative-evaluation-with-classical-monomelic-amyotrophy-and-other-motor-neuron-disease
#15
Deb Kumar Boruah, Shantiranjan Sanyal, Arjun Prakash, Sashidhar Achar, Dhabal D Dhingani, Binod Sarma
Background: The aim of the study was to evaluate the magnetic resonance imaging (MRI) findings in bilateral symmetrical Hirayama disease and find out MRI features which are probably more indicative of symmetrical Hirayama disease, thereby help in differentiating this entity from other motor neuron disease (MND). Methods: This prospective as well as retrospective study was carried out from December 2010 to September 2016 in a tertiary care center of northeast India on 92 patients with Hirayama disease. Only 19 patients having bilateral symmetric upper limb involvement at the time of presentation were included in this study sample...
July 6, 2017: Iranian Journal of Neurology
https://www.readbyqxmd.com/read/29113824/comparative-analysis-of-different-cell-systems-for-zika-virus-zikv-propagation-and-evaluation-of-anti-zikv-compounds-in-vitro
#16
Ilaria Vicenti, Adele Boccuto, Alessia Giannini, Filippo Dragoni, Francesco Saladini, Maurizio Zazzi
A strong correlation between Zika virus (ZIKV) infection and severe neurological disease in newborns and occasionally adults has emerged in the Brazilian outbreak. Efficient human cell-based assays are required to test candidate inhibitors of ZIKV replication. The aim of this work was to investigate ZIKV propagation and quantification in different cell lines. The human (U87, A549, Huh7), mosquito (C6/36) and monkey (VERO E6) cell lines tested were all permissive to ZIKV infection. When assessed by plaque forming units (PFU) in three different target cell lines, the maximal production of ZIKV was achieved in Huh7 at day 3 post-infection (6...
November 4, 2017: Virus Research
https://www.readbyqxmd.com/read/29110476/certain-perfluoroalkyl-and-polyfluoroalkyl-substances-associated-with-aqueous-film-forming-foam-are-widespread-in-canadian-surface-waters
#17
Lisa A D'Agostino, Scott A Mabury
The presence of perfluoroalkyl and polyfluoroalkyl substances (PFASs) commonly associated with aqueous film forming foams (AFFFs) at sites without known AFFF contamination is a largely unexplored area, which may reveal widespread environmental contaminants requiring further investigation. Sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening for 23 classes of PFASs, followed by quantitative analysis was used to investigate surface waters from rural, urban, and AFFF-impacted sites in Canada...
November 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29099114/d%C3%A3-pteros-ectopar%C3%A3-sitos-hemat%C3%A3-fagos-%C3%A2-reservorios-potenciales-del-virus-del-dengue
#18
Álvaro Aguilar Setién, Anahí García Baltazar, Ignacio Olave Leyva, Mónica Salas Rojas, Vadim Pérez Koldenkova, Mariem Pérez-Peña García, Nidia Aréchiga Ceballos, Guillermo Gálvez Romero, Edgar Olivier López Villegas, Juan Bibiano Morales Malacara, Cenia Almazán Marín
Recently, the presence of antibodies and dengue virus (DV) RNA in neotropical wild mammals, including Desmodus rotundus, was reported. In a previous study, DV was also found in a high percentage (39.6%) of ectoparasitic hematophagous dipters specifics of these hematophagous bats. In order to verify the susceptibility of these ectoparasites to DV, in this work experimental infections with VD2 of organs explants of Strebla wiedemanni and of Melophagus ovinus were performed using C6/36 cells as control. Viral titers (UFP/mL) were determined at 0, 48 and 96 hrs pi...
2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/29098151/xbp1-mediated-bip-grp78-upregulation-copes-with-oxidative-stress-in-mosquito-cells-during-dengue-2-virus-infection
#19
Tien-Huang Chen, Yi-Hsuan Chiang, Jiun-Nan Hou, Chih-Chieh Cheng, Eny Sofiyatun, Cheng-Hsun Chiu, Wei-June Chen
Dengue viruses (DENVs) cause dengue fever which is an important mosquito-borne disease in tropical areas. Generally, DENV does not cause cellular damage in mosquito cells. However, alterations in cytosolic calcium ions ([Ca(2+)]cyt) and the mitochondrial membrane potential (MMP), as well as accumulated reactive oxygen species (ROS), including superoxide anions (O2(∙-)) and hydrogen peroxide (H2O2), can be detected in C6/36 cells with DENV2 infection. Evident upregulation of BiP/GRP78 also appeared at 24 h postinfection in DENV2-infected C6/36 cells...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29058107/zika-virus-isolated-from-mosquitoes-a-field-and-laboratory-investigation-in-china-2016
#20
Shihong Fu, Song Song, Hong Liu, Yuanyuan Li, Xiaolong Li, Xiaoyan Gao, Ziqian Xu, Guoping Liu, Dingming Wang, Zhenzao Tian, Jingzhu Zhou, Ying He, Wenwen Lei, Huanyu Wang, Bin Wang, Xiaoqing Lu, Guodong Liang
A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells (C6/36) and mammalian cells (BHK-21) for virus isolation. The results indicated that 40% (3,540/8,795) were Anopheles sinensis, 30% (2,700/8,795) were Culex pipiens quinquefasciatus, and 29% (2,530/8,795) were Armigeres subbalbeatus...
October 19, 2017: Science China. Life Sciences
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