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https://www.readbyqxmd.com/read/29143280/genome-sequencing-and-phylogenetic-analysis-of-banna-virus-genus-seadornavirus-family-reoviridae-isolated-from-culicoides
#1
Song Song, Yuanyuan Li, Shihong Fu, Wenwen Lei, Xiaofang Guo, Yun Feng, Xiaoyan Gao, Xiaolong Li, Zhonghua Yang, Ziqian Xu, Ying He, Huanyu Wang, Hongning Zhou, Bin Wang, Xiaoqing Lu, Guodong Liang
In an investigation of blood-sucking insects and arboviruses, a virus (YN12243) was isolated from Culicoides samples collected in the Sino-Burmese border region of Yunnan Province, China. The virus caused cytopathic effect (CPE) in C6/36 cells and passaged stably. Polyacrylamide gel analysis showed that the genome of YN12243 was composed of 12 segments of double-stranded RNA (dsRNA), with a distribution pattern of 6-6. The nucleotide and amino acid sequences of the coding region (1‒12 segments) were 17,803 bp and 5,925 amino acids in length, respectively...
October 26, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/29133118/functional-characterization-of-a-serine-protease-inhibitor-modulated-in-the-infection-of-the-aedes-aegypti-with-dengue-virus
#2
Tatiane Sanches Soares, Boris Luis Rodriguez Gonzalez, Ricardo José Soares Torquato, Francisco Jose Alves Lemos, André L Costa-da-Silva, Margareth de Lara Capurro Guimarães, Aparecida Sadae Tanaka
During feeding with blood meal, female Aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and Zika viruses. Dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. In the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. However, little information is available about these molecules in the Ae...
November 10, 2017: Biochimie
https://www.readbyqxmd.com/read/29114369/bimelic-symmetric-hirayama-disease-spectrum-of-magnetic-resonance-imaging-findings-and-comparative-evaluation-with-classical-monomelic-amyotrophy-and-other-motor-neuron-disease
#3
Deb Kumar Boruah, Shantiranjan Sanyal, Arjun Prakash, Sashidhar Achar, Dhabal D Dhingani, Binod Sarma
Background: The aim of the study was to evaluate the magnetic resonance imaging (MRI) findings in bilateral symmetrical Hirayama disease and find out MRI features which are probably more indicative of symmetrical Hirayama disease, thereby help in differentiating this entity from other motor neuron disease (MND). Methods: This prospective as well as retrospective study was carried out from December 2010 to September 2016 in a tertiary care center of northeast India on 92 patients with Hirayama disease. Only 19 patients having bilateral symmetric upper limb involvement at the time of presentation were included in this study sample...
July 6, 2017: Iranian Journal of Neurology
https://www.readbyqxmd.com/read/29113824/comparative-analysis-of-different-cell-systems-for-zika-virus-zikv-propagation-and-evaluation-of-anti-zikv-compounds-in-vitro
#4
Ilaria Vicenti, Adele Boccuto, Alessia Giannini, Filippo Dragoni, Francesco Saladini, Maurizio Zazzi
A strong correlation between Zika virus (ZIKV) infection and severe neurological disease in newborns and occasionally adults has emerged in the Brazilian outbreak. Efficient human cell-based assays are required to test candidate inhibitors of ZIKV replication. The aim of this work was to investigate ZIKV propagation and quantification in different cell lines. The human (U87, A549, Huh7), mosquito (C6/36) and monkey (VERO E6) cell lines tested were all permissive to ZIKV infection. When assessed by plaque forming units (PFU) in three different target cell lines, the maximal production of ZIKV was achieved in Huh7 at day 3 post-infection (6...
November 4, 2017: Virus Research
https://www.readbyqxmd.com/read/29110476/certain-perfluoroalkyl-and-polyfluoroalkyl-substances-associated-with-aqueous-film-forming-foam-are-widespread-in-canadian-surface-waters
#5
Lisa A D'Agostino, Scott A Mabury
The presence of perfluoroalkyl and polyfluoroalkyl substances (PFASs) commonly associated with aqueous film forming foams (AFFFs) at sites without known AFFF contamination is a largely unexplored area, which may reveal widespread environmental contaminants requiring further investigation. Sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening for 23 classes of PFASs, followed by quantitative analysis was used to investigate surface waters from rural, urban, and AFFF-impacted sites in Canada...
November 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29099114/d%C3%A3-pteros-ectopar%C3%A3-sitos-hemat%C3%A3-fagos-%C3%A2-reservorios-potenciales-del-virus-del-dengue
#6
Álvaro Aguilar Setién, Anahí García Baltazar, Ignacio Olave Leyva, Mónica Salas Rojas, Vadim Pérez Koldenkova, Mariem Pérez-Peña García, Nidia Aréchiga Ceballos, Guillermo Gálvez Romero, Edgar Olivier López Villegas, Juan Bibiano Morales Malacara, Cenia Almazán Marín
Recently, the presence of antibodies and dengue virus (DV) RNA in neotropical wild mammals, including Desmodus rotundus, was reported. In a previous study, DV was also found in a high percentage (39.6%) of ectoparasitic hematophagous dipters specifics of these hematophagous bats. In order to verify the susceptibility of these ectoparasites to DV, in this work experimental infections with VD2 of organs explants of Strebla wiedemanni and of Melophagus ovinus were performed using C6/36 cells as control. Viral titers (UFP/mL) were determined at 0, 48 and 96 hrs pi...
2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/29098151/xbp1-mediated-bip-grp78-upregulation-copes-with-oxidative-stress-in-mosquito-cells-during-dengue-2-virus-infection
#7
Tien-Huang Chen, Yi-Hsuan Chiang, Jiun-Nan Hou, Chih-Chieh Cheng, Eny Sofiyatun, Cheng-Hsun Chiu, Wei-June Chen
Dengue viruses (DENVs) cause dengue fever which is an important mosquito-borne disease in tropical areas. Generally, DENV does not cause cellular damage in mosquito cells. However, alterations in cytosolic calcium ions ([Ca(2+)]cyt) and the mitochondrial membrane potential (MMP), as well as accumulated reactive oxygen species (ROS), including superoxide anions (O2(∙-)) and hydrogen peroxide (H2O2), can be detected in C6/36 cells with DENV2 infection. Evident upregulation of BiP/GRP78 also appeared at 24 h postinfection in DENV2-infected C6/36 cells...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29058107/zika-virus-isolated-from-mosquitoes-a-field-and-laboratory-investigation-in-china-2016
#8
Shihong Fu, Song Song, Hong Liu, Yuanyuan Li, Xiaolong Li, Xiaoyan Gao, Ziqian Xu, Guoping Liu, Dingming Wang, Zhenzao Tian, Jingzhu Zhou, Ying He, Wenwen Lei, Huanyu Wang, Bin Wang, Xiaoqing Lu, Guodong Liang
A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells (C6/36) and mammalian cells (BHK-21) for virus isolation. The results indicated that 40% (3,540/8,795) were Anopheles sinensis, 30% (2,700/8,795) were Culex pipiens quinquefasciatus, and 29% (2,530/8,795) were Armigeres subbalbeatus...
October 19, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/29053756/comprehensive-and-comparative-lipidome-analysis-of-vitis-vinifera-l-cv-pinot-noir-and-japanese-indigenous-v-vinifera-l-cv-koshu-grape-berries
#9
COMPARATIVE STUDY
Kayo Arita, Taro Honma, Shunji Suzuki
Vitis vinifera cv. Koshu is an indigenous grape cultivar that has been cultivated for more than a thousand years in Japan and one of the most important cultivars in white winemaking. To improve Koshu wine quality, it is necessary to identify the metabolites in Koshu berry. We conducted a comprehensive and comparative lipidome analysis of Koshu and Pinot Noir berries cultivated in the same location in Japan using GC-MS/MS for fatty acids and LC-MS for glycerolipids and glycerophospholipids. Koshu skins and juices contained 22 and 19 fatty acids, respectively, whereas 23 and 20 fatty acids were detected in Pinot Noir skins and juices...
2017: PloS One
https://www.readbyqxmd.com/read/29048274/isolation-and-characterization-of-a-new-iflavirus-from-armigeres-spp-mosquitoes-in-the-philippines
#10
Daisuke Kobayashi, Haruhiko Isawa, Ryosuke Fujita, Katsunori Murota, Kentaro Itokawa, Yukiko Higa, Yukie Katayama, Toshinori Sasaki, Tetsuya Mizutani, Shiroh Iwanaga, Nobuo Ohta, Arlene Garcia-Bertuso, Kyoko Sawabe
During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1...
November 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29039731/patchy-dna-forms-of-the-zika-virus-rna-genome-are-generated-following-infection-in-mosquito-cell-cultures-and-in-mosquitoes
#11
Dilip K Nag, Laura D Kramer
Zika virus (ZIKV) is a mosquito-borne flavivirus and has historically been reported to cause mild symptomatic diseases during human infections. More recently, the explosion of microcephaly among infants born to ZIKV-infected women has made ZIKV a global public health concern. While ZIKV causes acute human diseases, infections of vector mosquitoes are basically non-pathogenic, allowing persistent infections and conferring lifelong ability to transmit the virus. Recent studies have revealed that DNA forms of arboviral RNA genomes play a significant role in viral persistence in mosquitoes...
October 17, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29031868/prevalence-and-risk-factors-for-chronic-venous-disease-in-the-general-russian-population
#12
Igor A Zolotukhin, Evgeny I Seliverstov, Yuri N Shevtsov, Ilona P Avakiants, Aleksey S Nikishkov, Andrey M Tatarintsev, Alexander I Kirienko
OBJECTIVE/BACKGROUND: The aim was to establish the prevalence of chronic venous disease (CVD) and its risk factors in the general population. METHODS: This was a population based, cross sectional study. In total, 703 residents aged > 18 years from the rural community of Kryukovo (Central Russia) were enrolled. Medical history was taken and clinical examination performed, documenting venous signs/symptoms. The CEAP classification of the most affected limb was used...
October 11, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29030319/coordination-of-different-ligands-to-copper-ii-and-cobalt-iii-metal-centers-enhances-zika-virus-and-dengue-virus-loads-in-both-arthropod-cells-and-human-keratinocytes
#13
Shovan Dutta, Michael J Celestine, Supreet Khanal, Alexis Huddleston, Colin Simms, Jessa Faye Arca, Amlan Mitra, Loree Heller, Piotr J Kraj, Michel Ledizet, John F Anderson, Girish Neelakanta, Alvin A Holder, Hameeda Sultana
Trace elements such as copper and cobalt have been associated with virus-host interactions. However, studies to show the effect of conjugation of copper(II) or cobalt(III) metal centers to thiosemicarbazone ligand(s) derived from either food additives or mosquito repellent such as 2-acetylethiazole or citral, respectively, on Zika virus (ZIKV) or dengue virus (serotype 2; DENV2) infections have not been explored. In this study, we show that four compounds comprising of thiosemicarbazone ligand derived from 2-acetylethiazole viz...
January 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29017305/spontaneous-epidural-hematoma-associated-with-venous-phlebolith-in-cervical-spine-and-possible-pathogenesis
#14
Dong Hwan Kim, Dong Ha Kim, Kyoung Hyup Nam, Byung Kwan Choi, In Ho Han
Although the etiology of spontaneous spinal epidural hematoma (SSEH) is unclear, SSEH is known to be associated with anticoagulants, coagulopathy, vascular malformation, hypertension, and pregnancy. However, no report has been issued on the relation between SSEH and venous phlebolith. Here, the authors present an extremely rare case of SSEH associated with phlebolith in the cervical spine and suggest a possible pathogenesis. A 36-year-old man without any relevant medical history presented with neck pain and numbness and severe radiating pain on the left arm...
September 2017: Korean Journal of Spine
https://www.readbyqxmd.com/read/28964388/-health-related-quality-of-life-in-chronic-venous-disease-systematic-literature-review
#15
N Zenati, J L Bosson, S Blaise, P Carpentier
OBJECTIVE: Excepting life-threatening situations, improvement of health-related quality-of-life is the main therapeutic goal in chronic disease. The purpose of this review was to study the different ways of assessing Quality-of-Life (QoL) in patients with chronic venous disease (CVD) (CEAP classes C3 to C6). METHODS: A literature search was conducted with three databases: MEDLINE, WEB OF SCIENCE and COCHRANE LIBRARY in order to identify articles with the PRISMA reporting guidelines...
October 2017: Journal de Médecine Vasculaire
https://www.readbyqxmd.com/read/28930151/perk-signal-modulated-protein-translation-promotes-the-survivability-of-dengue-2-virus-infected-mosquito-cells-and-extends-viral-replication
#16
Jiun-Nan Hou, Tien-Huang Chen, Yi-Hsuan Chiang, Jing-Yun Peng, Tsong-Han Yang, Chih-Chieh Cheng, Eny Sofiyatun, Cheng-Hsun Chiu, Chuan Chiang-Ni, Wei-June Chen
Survival of mosquitoes from dengue virus (DENV) infection is a prerequisite of viral transmission to the host. This study aimed to see how mosquito cells can survive the infection during prosperous replication of the virus. In C6/36 cells, global protein translation was shut down after infection by DENV type 2 (DENV2). However, it returned to a normal level when infected cells were treated with an inhibitor of the protein kinase RNA (PKR)-like ER kinase (PERK) signaling pathway. Based on a 7-Methylguanosine 5'-triphosphate (m7GTP) pull-down assay, the eukaryotic translation initiation factor 4F (eIF4F) complex was also identified in DENV2-infected cells...
September 20, 2017: Viruses
https://www.readbyqxmd.com/read/28926632/risk-analysis-of-inter-species-reassortment-through-a-rift-valley-fever-phlebovirus-mp-12-vaccine-strain
#17
Hoai J Ly, Nandadeva Lokugamage, Shoko Nishiyama, Tetsuro Ikegami
Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula. The causative agent, Rift Valley fever phlebovirus (RVFV), belongs to the genus Phlebovirus in the family Phenuiviridae and causes high rates of abortions in ruminants, and hemorrhagic fever, encephalitis, or blindness in humans. Viral maintenance by mosquito vectors has led to sporadic RVF outbreaks in ruminants and humans in endemic countries, and effective vaccination of animals and humans may minimize the impact of this disease...
2017: PloS One
https://www.readbyqxmd.com/read/28898286/structural-investigation-of-c6-36-and-vero-cell-cultures-infected-with-a-brazilian-zika-virus
#18
Debora Ferreira Barreto-Vieira, Fernanda Cunha Jácome, Marcos Alexandre Nunes da Silva, Gabriela Cardoso Caldas, Ana Maria Bispo de Filippis, Patrícia Carvalho de Sequeira, Elen Mello de Souza, Audrien Alves Andrade, Pedro Paulo de Abreu Manso, Gisela Freitas Trindade, Sheila Maria Barbosa Lima, Ortrud Monika Barth
Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28857522/validation-of-the-croatian-version-of-civiq-quality-of-life-questionnaire-in-patients-with-chronic-venous-disorders
#19
Tamara Sinožić, Ksenija Baždarić, Dina Šverko, Alen Ružić, Milica Katić
AIM: To test the psychometric properties of the Croatian version of the Chronic Venous Insufficiency Quality of Life (CIVIQ) Questionnaire and to assess the quality of life in patients with chronic venous disorders of all stages. METHODS: This cross-sectional study performed between 2014 and 2015 in a private family practice assessed the factorial validity, cross-sectional validity, and reliability of the Croatian CIVIQ 20-item questionnaire completed by 428 adult patients (78% women) with chronic venous disorders classified according to the Clinical-Etiologic-Anatomic-Pathophysiologic (CEAP) C classification as stages C1-C6...
August 31, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28830495/new-insights-into-hcv-replication-in-original-cells-from-aedes-mosquitoes
#20
Catherine Fallecker, Alban Caporossi, Yassine Rechoum, Frederic Garzoni, Sylvie Larrat, Olivier François, Pascal Fender, Patrice Morand, Imre Berger, Marie-Anne Petit, Emmanuel Drouet
BACKGROUND: The existing literature about HCV association with, and replication in mosquitoes is extremely poor. To fill this gap, we performed cellular investigations aimed at exploring (i) the capacity of HCV E1E2 glycoproteins to bind on Aedes mosquito cells and (ii) the ability of HCV serum particles (HCVsp) to replicate in these cell lines. METHODS: First, we used purified E1E2 expressing baculovirus-derived HCV pseudo particles (bacHCVpp) so we could investigate their association with mosquito cell lines from Aedes aegypti (Aag-2) and Aedes albopictus (C6/36)...
August 22, 2017: Virology Journal
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