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https://www.readbyqxmd.com/read/28595631/isolation-of-tibet-orbivirus-from-culicoides-and-associated-infections-in-livestock-in-yunnan-china
#1
Jinglin Wang, Huachun Li, Yuwen He, Yang Zhou, Aiguo Xin, Defang Liao, Jinxin Meng
BACKGROUND: Culicoides-borne orbiviruses, such as bluetongue virus (BTV) and African horse sickness virus (AHSV), are important pathogens that cause animal epidemic diseases leading to significant loss of domestic animals. This study was conducted to identify Culicoides-borne arboviruses and to investigate the associated infections in local livestock in Yunnan, China. METHODS: Culicoides were collected overnight in Mangshi City using light traps during August 2013...
June 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28533943/etiology-and-treatment-of-amyotrophic-lateral-sclerosis
#2
Hernando Rafael, Juan Oscar David, Antonio Santiago Vilca
BACKGROUND: To date all researchers conclude that the etiology of Amyotrophic lateral sclerosis (ALS) is not known. On the contrary, since August 2009, we believe that disease is of ischemic origin in the anterior surface of the medulla oblongata. MATERIAL AND METHOD: We present our surgical experience into 45 patients with ALS (bulbar form in 36 cases and spinal form in 9). Preoperative MRI scans revealed microinfarcts in the medulla oblongata and/or cervical cord...
2017: American Journal of Neurodegenerative Disease
https://www.readbyqxmd.com/read/28514499/surface-anatomy-of-major-anatomical-landmarks-of-the-neck-in-an-adult-population-a-ct-evaluation-of-vertebral-level
#3
Masroor Badshah, Roger Soames, Muhammad Ibrahim, Muhammad Jaffar Khan, Adnan Khan
To compare the projectional surface anatomy of healthy individuals in an adult population with those with a thyroid mass, using computed tomography (CT). Sixteen slice CT images of 101 individuals were analyzed using a 32-bit Radiant DICOM viewer to establish the relationships among major anatomical landmarks in the neck and their vertebral levels. The structures investigated included: hard palate (HP), hyoid bone (HB) including body and lesser horns, soft palate (SP), thyroid gland (TG) (both superior and inferior poles), thyroid gland anteroposterior (APD) and superoinferior (SID) diameters, thyroid isthmus (TI) superoinferior dimension, epiglottis, vertebral arteries (right and left), and both right and left parotid glands (superior and inferior extents)...
May 17, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28513149/vertical-profiles-sources-and-transport-of-pfass-in-the-arctic-ocean
#4
Leo W Y Yeung, Clifton Dassuncao, Scott Mabury, Elsie M Sunderland, Xianming Zhang, Rainer Lohmann
The relative importance of atmospheric versus oceanic transport for poly- and perfluorinated alkyl substances (PFASs) reaching the Arctic Ocean is not well understood. Vertical profiles from the Central Arctic Ocean and shelf water, snow and meltwater samples were collected in 2012; 13 PFASs (C6-C12 PFCAs; C6, 8, 10 PFSAs; MeFOSAA and EtFOSAA; and FOSA) were routinely detected (range: <5-343 pg/L). PFASs were only detectable above 150 m depth in the polar mixed layer (PML) and halocline. Enhanced concentrations were observed in snow and meltpond samples, implying atmospheric deposition as an important source of PFASs...
June 5, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28495241/the-natural-course-of-prevertebral-soft-tissue-swelling-after-anterior-cervical-spine-surgery-how-long-will-it-last
#5
Seok Woo Kim, Chulyoung Jang, Myung Ho Yang, Seonjong Lee, Je Hyun Yoo, Yoon Hae Kwak, Ji Hyo Hwang
BACKGROUND CONTEXT: Prevertebral soft tissue swelling (PSTS) after anterior cervical spine surgery (ACSS) has been regarded as one of the critical complications that cause airway obstruction. Still, however, no research has dealt with how PSTS returns to pre-surgery status after ACSS; most recommendations are being performed without information about its natural course, focusing on acute-phase swelling after surgery. PURPOSE: To examine how long post-surgery PSTS lasts and when it returns to its pre-surgery state; to analyze the actual influence of a number of factors to observe the natural progress of post-surgery PSTS...
May 8, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28488954/isolation-of-a-novel-orthobunyavirus-from-bat-flies-eucampsipoda-africana
#6
Petrus Jansen van Vuren, Michael R Wiley, Gustavo Palacios, Nadia Storm, Wanda Markotter, Monica Birkhead, Alan Kemp, Janusz T Paweska
The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans...
May 11, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28445704/development-of-standard-methods-for-zika-virus-propagation-titration-and-purification
#7
Sharton Vinicius Antunes Coelho, Rômulo Leão Silva Neris, Michelle Premazzi Papa, Laila Castro Schnellrath, Lana Monteiro Meuren, Diogo A Tschoeke, Luciana Leomil, Brunno Renato Farias Verçoza, Milene Miranda, Fabiano L Thompson, Andrea Thompson Da Poian, Thiago Moreno L Souza, Fabiana Avila Carneiro, Clarissa R Damaso, Iranaia Assunção-Miranda, Luciana Barros de Arruda
The emergence of Zika virus (ZIKV) infection has stimulated several research groups to study and collaborate to understand virus biology and pathogenesis. These efforts may assist with the development of antiviral drugs, vaccines and diagnostic tests, as well as to promote advancements in public health policies. Here, we aim to develop standard protocols for propagation, titration, and purification of ZIKV strains, by systematically testing different cell types, kinetics, multiplicity of infection and centrifugation protocols...
April 23, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28434207/maternal-rates-of-lipolysis-and-glucose-production-in-late-pregnancy-are-independently-related-to-fetal-weight
#8
Barbro Diderholm, Kathryn Beardsall, Peter Murgatroyd, Christoph Lees, Jan Gustafsson, David Dunger
OBJECTIVE: Associations between maternal glucose levels and increased fetal growth are well established and independent relationships with maternal weight, weight gain and insulin resistance are also observed. The relative roles of lipolysis and glucose production in the determination of these observations remain unclear. DESIGN: We examined, through detailed physiological studies, the relationship between maternal late gestational energy substrate production (glucose and glycerol), maternal weight and weight gain, and estimated fetal size in the third trimester...
April 23, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28404849/quantitative-proteomic-analysis-of-mosquito-c6-36-cells-reveals-host-proteins-involved-in-zika-virus-infection
#9
Qi-Lin Xin, Cheng-Lin Deng, Xi Chen, Jun Wang, Shao-Bo Wang, Wei Wang, Fei Deng, Bo Zhang, Gengfu Xiao, Lei-Ke Zhang
Zika virus (ZIKV) is an emerging arbovirus belonging to the genus Flavivirus of the family Flaviviridae During replication processes, flavivirus will manipulate host cell systems to facilitate their replication, while the host cells will activate antiviral responses. Identification of host proteins involved in flavivirus replication process may lead to the discovery of antiviral targets. Mosquito Aedes aegypti and Aedes albopictus are epidemiologically important vectors for ZIKV, and effective restrictions of ZIKV replication in mosquitoes will be vital in controlling the spread of virus...
April 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28401498/identification-of-subgroups-of-patients-with-tension-type-headache-with-higher-widespread-pressure-pain-hyperalgesia
#10
César Fernández-de-Las-Peñas, Elena Benito-González, María Palacios-Ceña, Kelun Wang, Matteo Castaldo, Lars Arendt-Nielsen
BACKGROUND: Identification of subgroups of patients with different levels of sensitization and clinical features can help to identify groups at risk and the development of better therapeutic strategies. The aim of this study was to identify subgroups of patients with tension type headache (TTH) with different levels of sensitization, clinical pain features, and psychological outcomes. METHODS: A total of 197 individuals with TTH participated. Headache intensity, frequency, and duration and medication intake were collected with a 4-weeks diary...
December 2017: Journal of Headache and Pain
https://www.readbyqxmd.com/read/28377343/-three-dimensional-morphology-of-c6-36-cells-infected-by-dengue-virus-a-study-based-on-digital-holographic-microscopy
#11
Jian-Hai Yu, Xu-Ling Liu, Yu-Jing Liu, Xiao-En He, Yuan Hui, Bao Zhang, Li Zhu, Wei Zhao
OBJECTIVE: To monitor the 3-dimensional (3D) morphological changes of C6/36 cells during dengue virus (DENV) infection using a live-cell imaging technique based on digital holographic microscopy and provide clues for better understanding the mechanisms of DENV infection. METHODS: C6/36 cells were seeded in 6-well plates to determine the optimal imaging density under a holographic cell imager, and the morphological changes of the cells were recorded in response to a culture temperature change from 28 degrees celsius; to 37 degrees celsius; C6/36 cells were infected with 4 DENV strains with different serotypes at 28 degrees celsius; and incubated at 37 degrees celsius; for 24 h, and the 3D holograms and relevant morphological parameters were recorded at different time points using HoloMonitor M4 holographic cell imaging and analysis system...
March 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28352205/syringomyelia-presenting-with-unilateral-optic-neuropathy-a-case-report
#12
Qi Zhe Ngoo, Evelyn Li Min Tai, Wan Hazabbah Wan Hitam
PURPOSE: In this case report, we present two cases of syringomyelia with optic neuropathy. FINDINGS: In Case 1, a 36-year-old Malay lady presented to our clinic with acute onset of blurring of vision in her left eye that she experienced since past 1 month. She was diagnosed with syringomyelia 12 years ago and was on conservative management. Her visual acuity was 6/6 in the right eye and counting fingers at 1 m in the left. There was a positive relative afferent pupillary defect in her left eye...
2017: International Medical Case Reports Journal
https://www.readbyqxmd.com/read/28343780/propagation-of-brazilian-zika-virus-strains-in-static-and-suspension-cultures-using-vero-and-bhk-cells
#13
Alexander Nikolay, Leda R Castilho, Udo Reichl, Yvonne Genzel
The recent spread of Zika virus (ZIKV) in the Americas and the Pacific has reached alarming levels in more than 60 countries. However, relatively little is known about the disease on a virological and epidemiological level and its consequences for humans. Accordingly, a large demand for in vitro derived Brazilian ZIKV material to support in vitro and in vivo studies has arisen. However, a prompt supply of ZIKV and ZIKV antigens cannot be guaranteed as the production of this virus typically using Vero or C6/36 cell lines remains challenging...
March 23, 2017: Vaccine
https://www.readbyqxmd.com/read/28320443/co-circulation-of-west-nile-virus-and-distinct-insect-specific-flaviviruses-in-turkey
#14
Koray Ergünay, Nadine Litzba, Annika Brinkmann, Filiz Günay, Yasemen Sarıkaya, Sırrı Kar, Serra Örsten, Kerem Öter, Cristina Domingo, Özge Erisoz Kasap, Aykut Özkul, Luke Mitchell, Andreas Nitsche, Bülent Alten, Yvonne-Marie Linton
BACKGROUND: Active vector surveillance provides an efficient tool for monitoring the presence or spread of emerging or re-emerging vector-borne viruses. This study was undertaken to investigate the circulation of flaviviruses. Mosquitoes were collected from 58 locations in 10 provinces across the Aegean, Thrace and Mediterranean Anatolian regions of Turkey in 2014 and 2015. Following morphological identification, mosquitoes were pooled and screened by nested and real-time PCR assays. Detected viruses were further characterised by sequencing...
March 20, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28315365/mosquito-cells-c6-36-fail-to-support-the-complete-replication-of-penaeus-merguiensis-hepandensovirus
#15
D Syahidah, J Elliman, C Constantinoiu, L Owens
Mosquito cell lines (C6/36) were reported in the literature to support the propagation of Penaeus monodon hepandensovirus (PmoHDV). We aim to evaluate the susceptibility and viral propagation of P. merguiensis hepandensovirus (PmeHDV) which is ∼22% different to PmoHDV in Aedes albopictus cell line (C6/36). Cellular changes in the infected cell culture were detected. Vacuole formation was seen in both infected and uninfected cell cultures. The average number of disrupted cellular membranes in the infected cells (presumptive dead cells) was significantly higher than that of uninfected cells at passage two (F=9...
March 15, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28268195/-candidatus-rickettsia-nicoyana-a-novel-rickettsia-species-isolated-from-ornithodoros-knoxjonesi-in-costa-rica
#16
Rolando D Moreira-Soto, Andrés Moreira-Soto, Eugenia Corrales-Aguilar, Ólger Calderón-Arguedas, Adriana Troyo
Rickettsiae are intracellular bacteria commonly associated with hematophagous arthropods. Most of them have been described in hard ticks, but some have been found in soft ticks. Here we report the detection and isolation of a new Rickettsia from Ornithodoros knoxjonesi larvae collected from Balantiopteryx plicata (Emballonuridae) in Nicoya, Costa Rica. Two ticks were processed to detect Rickettsia spp. genes gltA, ompA, ompB, and htrA by PCR. Part of the macerate was also inoculated into Vero E6 and C6/36 cell lines, and cells were evaluated by Giménez stain, indirect immunofluorescence assay (IFA), and PCR...
February 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28267608/analysis-of-the-mirna-profile-in-c6-36-cells-persistently-infected-with-dengue-virus-type-2
#17
Rodolfo Gamaliel Avila-Bonilla, Martha Yocupicio-Monroy, Laurence A Marchat, Mónica A De Nova-Ocampo, Rosa María Del Ángel, Juan Santiago Salas-Benito
Dengue virus (DENV) is the most important arbovirus in the world; DENV is transmitted by the Aedes genus of mosquitoes and can establish a life-long persistent infection in mosquitoes. However, the exact mechanism by which persistent infection is established remains unknown. In this study the differential expression of miRNAs was analysed by deep sequencing and RT-qPCR using a previously established C6/36-HT cell line persistently infected with DENV 2 (C6-L) as a model. miR-927, miR-87, miR-210, miR-2a-3p, miR-190 and miR-970 were up-regulated, whereas miR-252, miR-263a-3p, miR-92b, miR-10-5p miR-9a-5p, miR-9a-1, miR-124, miR-286a and miR-286b were down-regulated in C6-L cells compared with C6/36 cells acutely infected with the same virus or mock-infected cells...
March 4, 2017: Virus Research
https://www.readbyqxmd.com/read/28250641/traumatic-high-grade-spondylolisthesis-at-c7-t1-with-no-neurological-deficits-case-series-literature-review-and-biomechanical-implications
#18
Ha Son Nguyen, Hesham Soliman, Shekar Kurpad
Traumatic high-grade spondylolisthesis in subaxial cervical spine is frequently associated with acute spinal cord injury and quadriparesis. There have been rare cases where such pathology demonstrates minimal to no neurological deficits. Assessment of the underlying biomechanics may provide insight into the mechanism of injury and associated neurological preservation. Patient 1 is a 63-year-old female presenting after a motor vehicle collision with significant right arm pain without neurological deficits. Imaging demonstrated C7/T1 spondyloptosis, associated with a locked facet on the left at C6/7 and a locked facet on the right at C7/T1, with a fracture of the left C7 pedicle and right C7 lamina...
January 2017: Journal of Craniovertebral Junction and Spine
https://www.readbyqxmd.com/read/28210996/quantitative-evaluation-of-oxygen-metabolism-in-the-intratumoral-hypoxia-18-f-fluoromisonidazole-and-15-o-labelled-gases-inhalation-pet
#19
Tadashi Watabe, Yasukazu Kanai, Hayato Ikeda, Genki Horitsugi, Keiko Matsunaga, Hiroki Kato, Kayako Isohashi, Kohji Abe, Eku Shimosegawa, Jun Hatazawa
BACKGROUND: Intratumoral hypoxia is one of the resistant factors in radiotherapy and chemotherapy for cancer. Although it is detected by (18)F-fluoromisonidazole (FMISO) PET, the relationship between intratumoral hypoxia and oxygen metabolism has not been studied. The purpose of this study was to evaluate the intratumoral perfusion and oxygen metabolism in hypoxic regions using the rat xenograft model. Ten male Fischer rats with C6 glioma (body weight = 220 ± 15 g) were investigated with (18)F-FMISO PET and steady-state inhalation method of (15)O-labelled gases PET...
December 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28159012/identification-of-micrornas-expressed-in-the-midgut-of-aedes-albopictus-during-dengue-infection
#20
Jianxin Su, Chunxiao Li, Yingmei Zhang, Ting Yan, Xiaojuan Zhu, Minghui Zhao, Dan Xing, Yande Dong, Xiaoxia Guo, Tongyan Zhao
BACKGROUND: The midgut is the first barrier to dengue virus (DENV) infections of mosquitoes and therefore is a major bottleneck for the subsequent development of vector competence. However, the molecular mechanisms responsible for this barrier are unknown. RESULTS: We constructed three small RNA libraries from the midguts of adult Aedes albopictus females that had been fed on either sugar solution, an uninfected blood meal, or a blood meal infected with DENV-2, and112 conserved microRNAs represented by 173 miRNA sequences were identified, with 34 novel microRNAs predicted by Mireap, RNAfold and Sfold software...
February 3, 2017: Parasites & Vectors
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