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https://www.readbyqxmd.com/read/26794473/insect-cell-transformation-vectors-that-support-high-level-expression-and-promoter-assessment-in-insect-cell-culture
#1
Paul D Shirk, Richard B Furlong
A somatic transformation vector, pDP9, was constructed that provides a simplified means of producing permanently transformed cultured insect cells that support high levels of protein expression of foreign genes. The pDP9 plasmid vector incorporates DNA sequences from the Junonia coenia densovirus that are involved in integration of the densovirus in insect cell chromosomes and a promoter/enhancer system that results in high levels of expression. The plasmid also contains two markers that permit selection of transformed insect cells by antibiotic resistance or by cell-sorting for fluorescent protein expression...
January 2016: Plasmid
https://www.readbyqxmd.com/read/24027326/densovirus-crosses-the-insect-midgut-by-transcytosis-and-disturbs-the-epithelial-barrier-function
#2
Y Wang, A S Gosselin Grenet, I Castelli, G Cermenati, M Ravallec, L Fiandra, S Debaisieux, C Multeau, N Lautredou, T Dupressoir, Y Li, M Casartelli, M Ogliastro
Densoviruses are parvoviruses that can be lethal for insects of different orders at larval stages. Although the horizontal transmission mechanisms are poorly known, densoviral pathogenesis usually starts with the ingestion of contaminated food by the host. Depending on the virus, this leads to replication restricted to the midgut or excluding it. In both cases the success of infection depends on the virus capacity to enter the intestinal epithelium. Using the Junonia coenia densovirus (JcDNV) as the prototype virus and the lepidopteran host Spodoptera frugiperda as an interaction model, we focused on the early mechanisms of infection during which JcDNV crosses the intestinal epithelium to reach and replicate in underlying target tissues...
November 2013: Journal of Virology
https://www.readbyqxmd.com/read/17714462/regulation-of-junonia-coenia-densovirus-p9-promoter-expression
#3
P D Shirk, H Bossin, R B Furlong, J L Gillett
Transcriptional activity of the Junonia coenia densovirus (JcDNV) P9 promoter depends on a 557-bp sequence located within the overlapping 3' sequences for viral capsid and nonstructural genes. Utilizing a somatic transformation assay to assess JcDNV promoter activity in Drosophila melanogaster and Plodia interpunctella, viral sequences were subjected to deletional analysis. Removal of a 685-bp fragment reduced P9-driven expression to background levels. Inclusion of a second expression cassette demonstrated vector persistence and confirmed somatic transformation...
October 2007: Insect Molecular Biology
https://www.readbyqxmd.com/read/9817847/the-structure-of-an-insect-parvovirus-galleria-mellonella-densovirus-at-3-7-a-resolution
#4
A A Simpson, P R Chipman, T S Baker, P Tijssen, M G Rossmann
BACKGROUND: Parvoviruses infect vertebrates, insects and crustaceans. Many arthropod parvoviruses (densoviruses) are highly pathogenic and kill approximately 90% of the host larvae within days, making them potentially effective as selective pesticides. Improved understanding of densoviral structure and function is therefore desirable. There are four different initiation sites for translation of the densovirus capsid protein mRNA, giving rise to the viral proteins VP1 to VP4. Sixty copies of the common, C-terminal domain make up the ordered part of the icosahedral capsid...
November 15, 1998: Structure
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