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Colistin resistance

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https://www.readbyqxmd.com/read/28630206/rapid-and-consistent-evolution-of-colistin-resistance-in-xdr-pseudomonas-aeruginosa-during-morbidostat-culture
#1
Bianca Regenbogen, Matthias Willmann, Matthias Steglich, Boyke Bunk, Ulrich Nübel, Silke Peter, Richard A Neher
Colistin is a last resort antibiotic commonly used against multidrug-resistant strains of Pseudomonas aeruginosa To investigate the potential for in-situ evolution of resistance against colistin and to map the molecular targets of colistin resistance, we exposed two P. aeruginosa isolates to colistin using a continuous culture device known as morbidostat. As a result, colistin resistance reproducibly increased 10-fold within ten days, and 100-fold within 20 days, along with highly stereotypic, yet strain specific mutation patterns...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28630204/a-low-molecular-weight-alginate-oligosaccharide-disrupts-pseudomonal-microcolony-formation-and-enhances-antibiotic-effectiveness
#2
Manon F Pritchard, Lydia C Powell, Alison A Jack, Kate Powell, Konrad Beck, Hannah Florance, Julian Forton, Philip D Rye, Arne Dessen, Katja E Hill, David W Thomas
In chronic respiratory disease the formation of dense, 3-dimensional 'micro colonies' by Pseudomonas aeruginosa within the airway plays an important role in contributing to resistance to treatment. An in vitro biofilm model of pseudomonal microcolony formation using artificial sputum (AS) medium was established to study the effects of low molecular weight alginate oligomers (OligoG CF-5/20) on pseudomonal growth, microcolony formation and the efficacy of colistin. The studies employed clinical cystic fibrosis (CF) isolates (n=3) and reference non-mucoid and mucoid multi-drug resistant (MDR) CF isolates (n=7)...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28629229/colistin-resistance-associated-with-outer-membrane-protein-change-in%C3%A2-klebsiella-pneumoniae-and-enterobacter-asburiae
#3
Béla Kádár, Béla Kocsis, Ákos Tóth, Katalin Kristóf, Péter Felső, Béla Kocsis, Katalin Böddi, Dóra Szabó
In this study, outer membrane proteins (OMPs) of colistin-resistant Klebsiella pneumoniae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. pneumoniae sequence type 258 strains as well as one colistin-susceptible E. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. OMP analysis of each strain was performed by microchip method. Matrix-assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) investigation was carried out after separation of OMPs by two-dimensional gel electrophoresis and in-gel digestion...
June 20, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28628439/locally-acquired-mcr-1-in-escherichia-coli-australia-2011-and-2013
#4
Justin A Ellem, Andrew N Ginn, Sharon C-A Chen, John Ferguson, Sally R Partridge, Jonathan R Iredell
We identified discrete importation events of the mcr-1 gene on incompatibility group IncI2 plasmids in Escherichia coli isolated from patients in New South Wales, Australia, in 2011 and 2013. mcr-1 is present in a small minority of colistin-resistant Enterobacteriaceae and appears not to be established locally.
July 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28626539/resistance-to-colistin-in-klebsiella-pneumoniae-a-4-0-strain
#5
REVIEW
Guido Granata, Nicola Petrosillo
The global rise of multidrug-resistant gram-negative bacteria represents an increasing threat to patient safety. From the first observation of a carbapenem-resistant gram-negative bacteria a global spread of extended-spectrum beta-lactamases and carbapenemases producing Klebsiella pneumoniae has been observed. Treatment options for multidrug-resistant K. pneumoniae are actually limited to combination therapy with some aminoglycosides, tigecycline and to older antimicrobial agents. Unfortunately, the prevalence of colistin-resistant and tigecycline-resistant K...
May 31, 2017: Infectious Disease Reports
https://www.readbyqxmd.com/read/28624864/the-clinical-significance-of-pneumonia-in-patients-with-respiratory-specimens-harbouring-multidrug-resistant-pseudomonas-aeruginosa-a-5-year-retrospective-study-following-5667-patients-in-four-general-icus
#6
B Borgatta, S Gattarello, C A Mazo, A T Imbiscuso, M N Larrosa, M Lujàn, J Rello
Pseudomonas aeruginosa is the leading cause of pneumonia in intensive care units (ICUs), with multidrug-resistant (MDR) strains posing a serious threat. The aim of this study was to assess the clinical relevance of MDR Pseudomonas isolates in respiratory clinical specimens. A 5-year retrospective observational study in four medical-surgical ICUs from a referral hospital was carried out. Of 5667 adults admitted to the ICU, 69 had MDR-PA in respiratory samples: 31 were identified as having pneumonia (HAP/VAP): 21 ventilator-associated pneumonia (VAP) and ten hospital-acquired pneumonia (HAP)...
June 17, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28623298/constraint-based-modeling-identifies-new-putative-targets-to-fight-colistin-resistant-a-baumannii-infections
#7
Luana Presta, Emanuele Bosi, Leila Mansouri, Lenie Dijkshoorn, Renato Fani, Marco Fondi
Acinetobacter baumannii is a clinical threat to human health, causing major infection outbreaks worldwide. As new drugs against Gram-negative bacteria do not seem to be forthcoming, and due to the microbial capability of acquiring multi-resistance, there is an urgent need for novel therapeutic targets. Here we have derived a list of new potential targets by means of metabolic reconstruction and modelling of A. baumannii ATCC 19606. By integrating constraint-based modelling with gene expression data, we simulated microbial growth in normal and stressful conditions (i...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28621641/colistin-resistance-in-pseudomonas-aeruginosa-that-is-not-linked-to-arnb
#8
Eun Seon Chung, Ji-Young Lee, Ji-Young Rhee, Kwan Soo Ko
PURPOSE: It is known that the arnB (or pmrH) gene encoding uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase plays a critical role in colistin resistance in Pseudomonas aeruginosa through the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to lipid A. In this study, we attempted to obtain a colistin-resistant mutant from an arnB-deleted mutant through exposure to colistin. METHODOLOGY: We constructed an arnB deletion mutant (P5ΔarnB :: nptIII) from a colistin-susceptible strain (P5) by allelic replacement mutagenesis, and colistin-resistant mutants were selected in vitro using P5 and P5ΔarnB :: nptIII...
June 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28619166/surveillance-of-antimicrobial-resistance-among-escherichia-coli-from-chicken-and-swine-china-2008-2015
#9
Peng Zhang, Zhangqi Shen, Chunping Zhang, Li Song, Bing Wang, Jun Shang, Xiuying Yue, Zhina Qu, Xinnan Li, Liqin Wu, Yongjun Zheng, Anand Aditya, Yang Wang, Shixin Xu, Congming Wu
The objective of this study was to investigate the antimicrobial resistance trend in Escherichia coli from food animals in China. During 2008-2015, a total of 15,130 E. coli were isolated from chicken and swine from seven provinces. The susceptibilities of these isolates to nine classes of antimicrobial agents were determined using broth microdilution susceptibility method. The findings of this study include: (1) multi-drug resistance was highly prevalent in E. coli; (2) these E. coli isolates showed high resistant rate (>80%) to several old drugs, including ampicillin, tetracycline and sulfisoxazole; (3) increasing resistance to colistin, florfenicol and ceftiofur was observed; (4) the E...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28616507/the-first-report-of-escherichia-fergusonii-isolated-from-non-human-primates-in-africa
#10
Barbara Glover, Jeanette Wentzel, Akinbowale Jenkins, Moritz Van Vuuren
The aim of this study was to determine the resistance phenotypes of selected enteric bacteria isolated from non-human primates at a wildlife-human interface. Bacterial isolates from faecal samples of non-human primates at two wildlife rehabilitation centres in South Africa were screened for the presence of Escherichia coli. The biochemical characterisation of E. coli and E. coli-like bacteria revealed both adonitol positive and sorbitol negative strains - a unique characteristic of Escherichia fergusonii and Escherichia coli K99...
June 2017: One Health
https://www.readbyqxmd.com/read/28614296/selective-depletion-of-uropathogenic-e-coli-from-the-gut-by-a-fimh-antagonist
#11
Caitlin N Spaulding, Roger D Klein, Ségolène Ruer, Andrew L Kau, Henry L Schreiber, Zachary T Cusumano, Karen W Dodson, Jerome S Pinkner, Daved H Fremont, James W Janetka, Han Remaut, Jeffrey I Gordon, Scott J Hultgren
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) affect 150 million people annually. Despite effective antibiotic therapy, 30-50% of patients experience recurrent UTIs. In addition, the growing prevalence of UPEC that are resistant to last-line antibiotic treatments, and more recently to carbapenems and colistin, make UTI a prime example of the antibiotic-resistance crisis and emphasize the need for new approaches to treat and prevent bacterial infections. UPEC strains establish reservoirs in the gut from which they are shed in the faeces, and can colonize the periurethral area or vagina and subsequently ascend through the urethra to the urinary tract, where they cause UTIs...
June 14, 2017: Nature
https://www.readbyqxmd.com/read/28606644/comparison-of-methods-for-detection-of-plasmid-mediated-and-chromosomally-encoded-colistin-resistance-in-enterobacteriaceae
#12
Aurélie Jayol, Patrice Nordmann, Philippe Lehours, Laurent Poirel, Véronique Dubois
OBJECTIVES: Because of the emergence of plasmid-mediated (mcr-1 and mcr-2 genes) and chromosomally-encoded colistin resistance, reliable methods for detecting colistin resistance/susceptibility in routine laboratories are required. We evaluated the respective performances of the BD Phoenix automated system, the newly-developed Rapid Polymyxin NP test and the broth microdilution (BMD) reference method to detect colistin resistance in Enterobacteriaceae, and particularly those producing MCR-1 and MCR-2...
June 9, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28605445/evaluation-of-two-automated-systems-for-colistin-susceptibility-testing-of-carbapenem-resistant-acinetobacter-baumannii-clinical-isolates
#13
Sophia Vourli, Konstantina Dafopoulou, Georgia Vrioni, Athanassios Tsakris, Spyros Pournaras
Background: Colistin is commonly needed for the treatment of infections due to carbapenem-resistant Acinetobacter baumannii (CRAB) and the determination of its in vitro activity is obviously important. However, the accurate routine antimicrobial susceptibility testing (AST) of colistin is still challenging. The only acceptable method for colistin AST is broth microdilution (BMD); disc and gradient diffusion assays are inappropriate and the performance of semi-automated systems has not been validated...
June 12, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28604100/molecular-epidemiology-of-carbapenem-resistant-acinetobacter-baumannii-in-greece-an-extended-review-2000-2015
#14
Theodoros Karampatakis, Charalampos Antachopoulos, Athanassios Tsakris, Emmanuel Roilides
Carbapenem-resistant Acinetobacter baumannii (CRAB) is endemic in Greece. CRAB initially emerged in 2000 and since then, carbapenemases still have a crucial role in CRAB appearance, except for a few cases resulting from efflux pump or outer-membrane protein mechanisms. OXA-type carbapenemases present the highest prevalence worldwide and bla OXA-23-like and bla OXA-58-like are the most important genes found; VIM-yielding CRAB have also been detected, while a single CRAB isolate producing NDM has quite recently emerged in Greece...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28598315/prevalence-of-faecal-carriage-of-colistin-resistant-gram-negative-rods-in-a-university-hospital-in-western-france-2016
#15
Marion Saly, Aurelie Jayol, Laurent Poirel, Francis Megraud, Patrice Nordmann, Veronique Dubois
Plasmid-mediated and chromosomally-encoded colistin resistance is increasingly being reported worldwide. We aimed to determine the prevalence of faecal carriage of colistin-resistant Gram-negative rod isolates in a university hospital in western France. From February to May 2016, rectal swabs from 653 patients hospitalized in various clinical settings were recovered and subsequently screened for colistin resistance using the SuperPolymyxin medium. Antimicrobial susceptibilities were determined according to EUCAST guidelines...
June 9, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28596943/in-vitro-evaluation-of-the-colistin-carbapenem-combination-in-clinical-isolates-of-a-baumannii-using-the-checkerboard-etest-and-time-kill-curve-techniques
#16
Micheline A H Soudeiha, Elias A Dahdouh, Eid Azar, Dolla K Sarkis, Ziad Daoud
The worldwide increase in the emergence of carbapenem resistant Acinetobacter baumannii (CRAB) calls for the investigation into alternative approaches for treatment. This study aims to evaluate colistin-carbapenem combinations against Acinetobacter spp., in order to potentially reduce the need for high concentrations of antibiotics in therapy. This study was conducted on 100 non-duplicate Acinetobacter isolates that were collected from different patients admitted at Saint George Hospital-University Medical Center in Beirut...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28595854/erratum-to-intestinal-colonisation-with-extended-spectrum-cephalosporin-and-colistin-resistant-enterobacteriaceae-in-hiv-positive-individuals-in-switzerland-molecular-features-and-risk-factors-international-journal-of-antimicrobial-agents-49-4-2017-519-521
#17
Joao Pires, Odette J Bernasconi, Christoph Hauser, Regula Tinguely, Andrew Atkinson, Vincent Perreten, Valentina Dona, Andri Rauch, Hansjakob Furrer, Andrea Endimiani
No abstract text is available yet for this article.
June 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28594635/epidemiology-of-carbapenem-resistant-klebsiella-pneumoniae-bloodstream-infections-in-a-chinese-children-s-hospital-predominance-of-new-delhi-metallo-%C3%AE-lactamase-1
#18
Fang Dong, Ye Zhang, Kaihu Yao, Jie Lu, Lingyun Guo, Shuang Lyu, Ying Yang, Yan Wang, Hongyan Zheng, Wenqi Song, Gang Liu
The spread of carbapenem-resistant Klebsiella pneumoniae (CRKp) has become a significant problem worldwide; however, relevant data in children are limited. We performed a retrospective study to better understand the epidemiology of CRKp bloodstream infections at Beijing Children's Hospital. A total of 164 K. pneumoniae strains were collected from blood cultures between January 2011 and September 2014, of which 52 (31.7%) were CRKp strains. All 52 CRKp strains were multidrug resistant; 46 (88.5%) and 49 (94...
June 8, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28592552/colistin-and-polymyxin-b-susceptibility-testing-for-carbapenem-resistant-and-mcr-positive-enterobacteriaceae-comparison-of-sensititre-microscan-vitek-2-and-etest-with-broth-microdilution
#19
Ka Lip Chew, My-Van La, Raymond T P Lin, Jeanette W P Teo
Colistin and polymyxin B remain part of the last line of antibiotics for multi-drug-resistant Gram negative bacteria such as carbapenem-resistant-Enterobacteriaceae Current EUCAST-CLSI joint recommendations are for broth microdilution to be performed for MIC testing of colistin. Commercial susceptibility testing methods were evaluated and compared against reference broth microdilution (BMD), using a susceptible breakpoint of ≤2mg/L for both colistin and polymyxin B. Seventy-six Enterobacteriaceae were included, of which twenty-one were mcr-1 positive...
June 7, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28586429/hafnia-an-enterobacterial-genus-naturally-resistant-to-colistin-revealed-by-three-susceptibility-testing-methods
#20
Aurélie Jayol, Marion Saly, Patrice Nordmann, Armelle Ménard, Laurent Poirel, Véronique Dubois
Objectives: To determine the susceptibility to colistin of Hafnia alvei and Hafnia paralvei , and to compare methods for colistin resistance detection in the Hafnia genus. Methods: A collection of 25 Hafnia isolates was studied. Species were identified by using 16S rRNA gene sequencing with subsequent phylogeny analysis. Susceptibility to colistin was determined using the broth microdilution (BMD) reference method, the Phoenix automated system, the Rapid Polymyxin NP test, the Etest system and the disc diffusion method...
June 6, 2017: Journal of Antimicrobial Chemotherapy
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