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https://www.readbyqxmd.com/read/27892867/the-balb-c-mouse-infection-model-for-improving-the-haemophilus-parasuis-serotyping-scheme
#1
Jiang Yu, Yuyu Zhang, Yijun Du, Jun Li, Baohua Huang, Wenbo Sun, Xiaoyan Cong, Jun Peng, Sufang Ren, Lihui Gou, Jianli Shi, Zhi Chen, Lei Chen, Jianda Li, Jie Yang, Jiaqiang Wu, Jinbao Wang
The use of BALB/c mouse as an alternative model to study Haemophilus parasuis (HPS) infections was evaluated, supplying the serotyping scheme by comparing the pathogenicity of different serovar HPS in pigs and mice challenge using statistical analysis. Results showed that the pathogenicity of different serovar HPS in mouse was consistent with in pigs, proving that this model is a viable alternative to pigs. It provides a convenient methodology for determining the virulence of HPS strains.
October 24, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27889498/bloc-2-subunit-hps6-deficiency-affects-the-tubulation-and-secretion-of-von-willebrand-factor-from-mouse-endothelial-cells
#2
Jing Ma, Zhe Zhang, Lin Yang, Janos Kriston-Vizi, Daniel F Cutler, Wei Li
Hermansky-Pudlak syndrome (HPS) is a recessive disorder with bleeding diathesis, which has been linked to platelet granule defects. Both platelet granules and endothelial Weibel-Palade bodies (WPBs) are members of lysosome-related organelles (LROs) whose formation is regulated by HPS protein associated complexes such as BLOC (biogenesis of lysosome organelles complex) -1, -2, -3, AP-3 (adaptor protein complex-3) and HOPS (homotypic fusion and protein sorting complex). Von Willebrand factor (VWF) is critical to hemostasis, which is stored in a highly-multimerized form as tubules in the WPBs...
October 21, 2016: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/27885828/superionic-conductivity-in-hybrid-of-3-hydroxypropanesulfonic-acid-and-graphene-oxide
#3
Kosuke Wakata, Mohammad Razaul Karim, Ryo Ohtani, Masaaki Nakamura, Michio Koinuma, Shinya Hayami
Insertion of 3-hydroxypropanesulfonicacid (HPS) at graphene oxide (GO) interlayer results in high proton conductivity (10-2 - 10-1 Scm-1) due to the improvement in oxygen content, interlayer distance and water absorbing capacity. This result indicates that hydroxyalkylsulfonicacid can be a perfect guest molecules for improving the proton conductivity of carbon materials.
November 25, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27883370/epstein-barr-virus-associated-hemophagocytic-syndrome-after-scrub-typhus-infection
#4
Jeong Woo Hong, Hyun Seon You, Tae Won Lee, Won Yong Jo, Bo Ra Kim, Young Sun Suh, In Gyu Bae, Oh Hyun Cho
There have been a small number of cases of scrub typhus-associated hemophagocytic syndrome (HPS), most of which were treated successfully using adequate antibiotics. Here, we report a case of Epstein-Barr virus (EBV)-associated HPS after scrub typhus infection that was not improved using antirickettsial treatment. A 73-year-old male who had been diagnosed with scrub typhus according to an eschar and a positive serology was transferred to our institution because of a persistent fever despite 7-day doxycycline therapy...
November 8, 2016: Infection & Chemotherapy
https://www.readbyqxmd.com/read/27879294/cxcr2-is-involved-in-pulmonary-intravascular-macrophage-accumulation-and-angiogenesis-in-a-rat-model-of-hepatopulmonary-syndrome
#5
Xujiong Li, Yunxia Chen, Yongli Chang, Shufen Li, Zhongfu Zhao, HuiYing Zhang
Hepatopulmonary syndrome (HPS) is a lung complication in various liver diseases, with high incidence, poor prognosis and no effective non-surgical treatments in patients with hepatocirrhosis. Therefore, assessing HPS pathogenesis to explore proper therapy strategies is clinically relevant. In this study, male Sprague-Dawley rats underwent sham operation or common bile duct ligation (CBDL). Two weeks post-surgery, the following groups were setup for 2 weeks of treatment: Sham + normal saline, CBDL + CXCR2 antagonist SB225002, CBDL + TNF-α antagonist PTX, and CBDL + normal saline groups...
November 22, 2016: Clinical Science (1979-)
https://www.readbyqxmd.com/read/27870860/gene-expression-profile-of-high-ifn-%C3%AE-producers-stimulated-with-leishmania-braziliensis-identifies-genes-associated-with-cutaneous-leishmaniasis
#6
Marcia W Carneiro, Kiyoshi F Fukutani, Bruno B Andrade, Rebecca P Curvelo, Juqueline R Cristal, Augusto M Carvalho, Aldina Barral, Johan Van Weyenbergh, Manoel Barral-Netto, Camila I de Oliveira
BACKGROUND: The initial response to Leishmania parasites is essential in determining disease development or resistance. In vitro, a divergent response to Leishmania, characterized by high or low IFN-γ production has been described as a potential tool to predict both vaccine response and disease susceptibility in vivo. METHODS AND FINDINGS: We identified uninfected and healthy individuals that were shown to be either high- or low IFN-γ producers (HPs and LPs, respectively) following stimulation of peripheral blood cells with Leishmania braziliensis...
November 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27868241/high-intensity-focused-ultrasound-treatment-for-skin-ex-vivo-evaluation
#7
J-H Park, S-D Lim, S H Oh, J H Lee, U C Yeo
BACKGROUND/PURPOSE: High-intensity focused ultrasound (HIFU) has been used for skin tightening. However, there is a rising concern of irreversible adverse effects. Our aim was to evaluate the depth of thermal injury zone after HIFU energy passes through different condition. MATERIALS AND METHODS: To analyze the consistency of the HIFU device, phantom tests were performed. Simulations were performed on ex vivo porcine tissues to estimate the area of the thermal coagulation point (TCP) according to the applied energy and skin condition...
November 20, 2016: Skin Research and Technology
https://www.readbyqxmd.com/read/27863494/molecular-epidemiology-of-hydropericardium-syndrome-outbreak-associated-serotype-4-fowl-adenovirus-isolates-in-central-china
#8
Teng Zhang, Qianyue Jin, Peiyang Ding, Yinbiao Wang, Yongxiao Chai, Yafei Li, Xiao Liu, Jun Luo, Gaiping Zhang
In several parts of China, there have been a large number of hydropericardium syndrome (HPS) outbreaks caused by serotype 4 fowl adenovirus (FAdV-4) in broiler chickens since 2015. These outbreak-associated FAdV-4 strains were distinct from previous circulating strains which did not lead to severe HPS outbreaks. To better understand the molecular epidemiology of the currently circulating FAdV strains for effective diagnosis and treatment of HPS, we isolated 12 HPS outbreak-associated FAdV-4 strains from different regions in central China and investigated their molecular characteristics by performing phylogenetic analyses based on the hexon genes...
November 18, 2016: Virology Journal
https://www.readbyqxmd.com/read/27861366/increased-risk-of-nonalcoholic-fatty-liver-disease-with-occupational-stress-in-chinese-policemen-a-4-year-cohort-study
#9
Chen Li, Jing-Jing Xing, An-Qi Shan, Ling Leng, Jin-Chuan Liu, Song Yue, Hao Yu, Xi Chen, Feng-Shi Tian, Nai-Jun Tang
Nonalcoholic fatty liver disease (NAFLD) and occupational stress have been recognized as major public health concerns. We aimed to explore whether occupational stress was associated with NAFLD in a police population.A total of 6559 male police officers were recruited for this prospective study in April 2007. Among them, 2367 eligible subjects participated in follow-up from 2008 to 2011. NAFLD was diagnosed based on standard criteria. Occupational stress was evaluated by Occupational Stress Inventory-Revised scores...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27856532/management-of-hermansky-pudlak-syndrome-in-pregnancy-and-review-of-literature
#10
Freya Van Avermaete, Joke Muys, Yves Jacquemyn
We report on the obstetric outcome of a woman aged 27 years with Hermansky-Pudlak syndrome (HPS). She underwent a caesarean section after failed induction of labour. Platelet transfusion was administered in a set schedule for 36 hours, starting 2 hours before delivery. The child had good Apgar scores and there were no significant problems of prolonged bleeding during the procedure. 72 hours postpartum, a haematoma developed at the site of the wound, subsequently complicated by a secondary infection for which she received antibiotics...
November 17, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27852795/modifiable-lifestyle-factors-associated-with-risk-of-sessile-serrated-polyps-conventional-adenomas-and-hyperplastic-polyps
#11
James R Davenport, Timothy Su, Zhiguo Zhao, Helen G Coleman, Walter E Smalley, Reid M Ness, Wei Zheng, Martha J Shrubsole
OBJECTIVE: To identify modifiable factors associated with sessile serrated polyps (SSPs) and compare the association of these factors with conventional adenomas (ADs) and hyperplastic polyps (HPs). DESIGN: We used data from the Tennessee Colorectal Polyp Study, a colonoscopy-based case-control study. Included were 214 SSP cases, 1779 AD cases, 560 HP cases and 3851 polyp-free controls. RESULTS: Cigarette smoking was associated with increased risk for all polyps and was stronger for SSPs than for ADs (OR 1...
November 15, 2016: Gut
https://www.readbyqxmd.com/read/27849499/trauma-resuscitation-evaluation-times-and-correlating-human-patient-simulation-training-differences-what-is-the-standard
#12
Timothy J Bonjour, Grigory Charny, Robert E Thaxton
BACKGROUND: Rapid effective trauma resuscitations (TRs) decrease patient morbidity and mortality. Few studies have evaluated TR care times. Effective time goals and superior human patient simulator (HPS) training can improve patient survivability. OBJECTIVES: The purpose of this study was to compare live TR to HPS resuscitation times to determine mean incremental resuscitation times and ascertain if simulation was educationally equivalent. The study was conducted at San Antonio Military Medical Center, Department of Defense Level I trauma center...
November 2016: Military Medicine
https://www.readbyqxmd.com/read/27842614/social-factors-associated-with-psychological-distress-and-health-problems-among-elderly-members-of-a-disaster-affected-population-subgroup-analysis-of-a-1-year-post-disaster-survey-in-ishinomaki-area-japan
#13
Mariko Inoue, Kazue Yamaoka
OBJECTIVE: We aimed to investigate the influence of social factors, especially social support, on psychological distress and the prevalence of health problems (HPs) among victims living in their homes 14 to 21 months after the Great East Japan Earthquake and tsunami. METHODS: A subgroup analysis was performed for elderly participants of a cross-sectional household survey in Ishinomaki area, Japan. The prevalence of psychological HPs was assessed by using a psychological distress questionnaire (K6) and by recording the number of subjective HPs...
November 15, 2016: Disaster Medicine and Public Health Preparedness
https://www.readbyqxmd.com/read/27836972/danger-lurking-in-the-unknowns-structure-to-function-studies-of-hypothetical-protein-bleg1_2437-from-bacillus-lehensis-g1-alkaliphile-revealed-an-evolutionary-divergent-b3-metallo-beta-lactamase
#14
Soo Huei Tan, Yahaya M Normi, Adam Thean Chor Leow, Abu Bakar Salleh, Abdul Munir Abdul Murad, Nor Muhammad Mahadi, Mohd Basyaruddin Abdul Rahman
The effectiveness of β-lactam antibiotics as chemotherapeutic agents to treat bacterial infections is gradually threatened with the emergence of antibiotic resistance mechanism among pathogenic bacteria through the production metallo-β-lactamase (MBL). In this study, we discovered a novel hypothetical protein (HP) termed Bleg1_2437 from the genome of alkaliphilic Bacillus lehensis G1 which exhibited MBL-like properties of B3 subclass; but evolutionary divergent from other circulating B3 MBLs. Domain and sequence analysis of HP Bleg1_2437 revealed that it contains highly conserved Zn(2+)-binding residues such as H54, H56, D58, H59, H131 and H191, important for catalysis, similar with the subclass B3 of MBL...
October 25, 2016: Journal of Biochemistry
https://www.readbyqxmd.com/read/27829521/a-randomized-trial-to-assess-advancement-of-enteral-feedings-following-surgery-for-hypertrophic-pyloric-stenosis
#15
Troy A Markel, Melissa R Scott, Samantha M Stokes, Alan P Ladd
PURPOSE: The rate of feeding advancement following surgery for hypertrophic pyloric stenosis (HPS) affects length of stay. We hypothesized that: 1) a relaxed feeding regimen following pyloromyotomy would allow infants to achieve feeding goals more quickly without affecting postoperative emesis, and 2) preoperative metabolic derangements would impair the ability to advance feedings following pyloromyotomy. METHODS: A prospective, randomized trial compared two postoperative feeding methods...
October 14, 2016: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27821005/an-approach-to-mentoring-healthcare-play-specialist-students
#16
Rachel Brown, Mags Jubb
Healthcare play specialists (HPSs) provide therapeutic play programmes for children in healthcare settings. Each HPS student must have a mentor in practice, but most HPSs have received no formal training for their role. This article explores mentoring in the HPS service at Guy's and St Thomas' NHS Foundation Trust. A study day for HPSs was arranged to share best practice and discuss the challenges of mentoring students. Stronger links were built between the higher education institute that delivers the training to HPS students and the trust, and the HPSs were provided with a deeper understanding of what was required of them in their mentoring role...
November 8, 2016: Nursing Children and Young People
https://www.readbyqxmd.com/read/27796825/complete-response-to-post-transplant-lymphoproliferative-disorder-by-surgical-resection-and-rituximab-after-living-donor-liver-re-transplantation-for-recurrent-primary-sclerosing-cholangitis
#17
Koichiro Haruki, Hiroaki Shiba, Junichi Shimada, Norimitsu Okui, Tomonori Iida, Katsuhiko Yanaga
Post-transplant lymphoproliferative disorder (PTLD) is a life-threatening complication of solid organ transplantation. We herein report a case of PTLD after living-donor liver re-transplantation (reLDLT) for recurrent primary sclerosing cholangitis (PSC), for which complete response was achieved by surgical resection and rituximab. A 47-year-old man, who had undergone living-donor liver transplantation (LDLT) twice at age of 43 and 45 years for end-stage liver disease firstly for PSC and secondary for recurrent PSC, suffered liver dysfunction due to an acute cellular rejection (ACR) 17 months after reLDLT...
October 31, 2016: Clinical Journal of Gastroenterology
https://www.readbyqxmd.com/read/27795403/the-andes-virus-nucleocapsid-protein-directs-basal-endothelial-cell-permeability-by-activating-rhoa
#18
Elena E Gorbunova, Matthew J Simons, Irina N Gavrilovskaya, Erich R Mackow
: Andes virus (ANDV) predominantly infects microvascular endothelial cells (MECs) and nonlytically causes an acute pulmonary edema termed hantavirus pulmonary syndrome (HPS). In HPS patients, virtually every pulmonary MEC is infected, MECs are enlarged, and infection results in vascular leakage and highly lethal pulmonary edema. We observed that MECs infected with the ANDV hantavirus or expressing the ANDV nucleocapsid (N) protein showed increased size and permeability by activating the Rheb and RhoA GTPases...
October 25, 2016: MBio
https://www.readbyqxmd.com/read/27794071/treating-dehydration-at-home-avoids-healthcare-costs-associated-with-emergency-department-visits-and-hospital-readmissions-for-adult-patients-receiving-home-parenteral-support
#19
Denise Konrad, Scott Roberts, Mandy L Corrigan, Cindy Hamilton, Ezra Steiger, Donald F Kirby
BACKGROUND: Administration of home parenteral support (HPS) has proven to be cost-effective over hospital care. Avoiding hospital readmissions became more of a focus for healthcare institutions in 2012 with the implementation of the Affordable Care Act. In 2010, our service developed a protocol to treat dehydration at home for HPS patients by ordering additional intravenous fluids to be kept on hand and to focus patient education on the symptoms of dehydration. METHODS: A retrospective analysis was completed through a clinical management database to identify HPS patients with dehydration...
October 28, 2016: Nutrition in Clinical Practice
https://www.readbyqxmd.com/read/27791999/comparison-of-hemorheological-changes-in-patients-after-acute-coronary-events-intervention-and-ambulatory-rehabilitation
#20
Andras Toth, Barbara Sandor, Zsolt Marton, Gabor Kesmarky, Eszter Szabados, Daniel Kehl, Istvan Juricskay, Laszlo Czopf, Kalman Toth
During the past decades, our group have investigated the hemorheological parameters (HPs) of more than 1,000 patients with various forms of ischemic heart disease (IHD). Our data indicate that HPs are altered in patients with IHD and the extent of the alterations is in good correlation with the clinical severity of the disease. Our findings have also proven that HPs play a critical role in the pathogenesis of myocardial ischemia.The lack of regular exercise is an important cardiovascular risk factor. Regular physical activity - as part of the cardiovascular rehabilitation training program (CRP) - is recommended for the treatment of IHD and the prevention of first or further cardiovascular events...
October 27, 2016: Clinical Hemorheology and Microcirculation
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