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https://www.readbyqxmd.com/read/29908162/isolation-and-characterization-of-endothelial-colony-forming-cells-from-mononuclear-cells-of-rat-bone-marrow
#1
Shukui Yu, Zhongxuan Li, Wei Zhang, Zhengde Du, Ke Liu, Denghua Yang, Shusheng Gong
Transplantation of bone marrow-derived endothelial progenitor cells (BM-EPCs) has been used as a therapeutic strategy for vascular repair. However, it remains controversial whether BM-EPCs exhibit clonal endothelial colony-forming cell (ECFC) capacity, a characteristic of true EPCs. The aim of this study was to isolate and explore the cellular properties of BM-ECFCs. We isolated BM-ECFCs from rat bone marrow with high purity via an optimized method. This approach involved the removal of selective colonies based on the conventional differential adhesive culture method used to isolate ECFCs from peripheral and umbilical cord blood...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29901632/alloreactive-cytotoxic-t-lymphocyte-immunotherapy-treatment-of-a-patient-with-metastatic-prostate-cancer-a-case-report
#2
Junfeng Shi, Yi Chen, Yuetong Chen, Yunzhu Shen, Huanyu Zhao, Hui Sun, Jinfei Chen
RATIONALE: Cytotoxic T lymphocyte (CTL) immunotherapy is an autologous cellular immune therapy that has been approved for treating patients with malignant tumors. However, there is still limited information regarding the impact of CTL on metastatic prostate cancer (PC) patients with bone metastatic lesions. PATIENT CONCERNS: An 82-year-old male patient complained of interrupted urination, urination pain, and significant dysuria on November 24, 2014. Transurethral resection of the prostate (TURP) and postoperative pathological examination showed prostatic adenocarcinoma, and a SPECT/CT scan demonstrated multiple bone metastases...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29898960/antibody-inhibition-of-properdin-prevents-complement-mediated-intravascular-and-extravascular-hemolysis
#3
Damodar Gullipalli, Fengkui Zhang, Sayaka Sato, Yoshiyasu Ueda, Yuko Kimura, Madhu Golla, Takashi Miwa, Jianxiang Wang, Wen-Chao Song
Paroxysmal nocturnal hemoglobinuria (PNH) is a serious blood disorder characterized by dysregulated complement activation on blood cells. Eculizumab, the current standard therapy and a humanized anti-C5 mAb, relieves anemia and thrombosis symptoms of PNH patients by preventing complement-dependent intravascular hemolysis (IVH). However, up to 20% of PNH patients on long-term eculizumab treatment still suffer from significant anemia and are transfusion dependent because of extravascular hemolysis (EVH) of C3-opsonized PNH erythrocytes...
June 13, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29896558/are-reinfusion-drains-safe-to-use-with-periarticular-liposomal-bupivacaine-an-analysis-of-systemic-bupivacaine-toxicity
#4
Marc R Angerame, Gavin P Hart, Susan M Odum, Bryan D Springer
Background: Intraoperative periarticular injection (PAI) with local anesthetic is an important component of multimodal pain control in total joint arthroplasty (TJA). A potential risk of this practice is serum anesthetic toxicity resulting from the autotransfusion of blood collected from a reinfusion drain. The purpose of this study is to evaluate the levels of bupivacaine in blood collected in an autotransfusion system after use of a PAI in TJA. Methods: In this prospective study, each TJA patient had an identical PAI consisting of 20 cc of liposomal bupivacaine, 30 cc of 0...
June 2018: Arthroplasty Today
https://www.readbyqxmd.com/read/29888810/short-and-midterm-results-for-internal-jugular-vein-extension-for-short-right-renal-vein-kidney-transplant
#5
Tony Lu, Stephanie G Yi, Jean Bismuth, Richard J Knight, A Osama Gaber, Carlos F Bechara
Renal transplantation remains the definitive treatment for end stage renal disease (ESRD). The shorter renal vein in right donor nephrectomies is associated with higher incidence of technical failure. We present here our experience with autologous internal jugular vein (IJV) conduits to facilitate living-donor transplants. Six patients underwent right, living-donor kidney transplant with simultaneous IJV harvest over a one year period. All had bilateral jugular duplex scans preoperatively, and were placed on aspirin 81 mg postoperatively...
June 11, 2018: Clinical Transplantation
https://www.readbyqxmd.com/read/29871892/safety-and-immunogenicity-of-conjugate-quadrivalent-meningococcal-vaccination-after-hematopoietic-cell-transplantation
#6
Matthew P Cheng, Alisha Pandit, Joseph H Antin, Stephen R Walsh, Daisy Huynh, Irene M Ghobrial, Lindsey R Baden, Francisco M Marty, Nicolas C Issa
Immunization with the conjugated quadrivalent (serogroups A, C, Y, and W-135) meningococcal vaccine (MCV4) after hematopoietic cell transplantation (HCT) is recommended. However, immune responses to MCV4 have not been prospectively studied after HCT. We conducted a vaccine response study among 67 adults who received 1 MCV4 dose a year after autologous or allogeneic HCT from January to September 2014. Pre- and postvaccination serogroup serum bactericidal antibody (SBA) titers were measured a median of 57 days after vaccination...
June 12, 2018: Blood Advances
https://www.readbyqxmd.com/read/29860903/difference-in-serum-endostatin-levels-in-diabetic-patients-with-critical-limb-ischemia-treated-by-autologous-cell-therapy-or-percutaneous-transluminal-angioplasty
#7
Andrea Nemcova, Alexandra Jirkovska, Michal Dubsky, Libor Kolesar, Robert Bem, Vladimira Fejfarova, Anna Pysna, Veronika Woskova, Jelena Skibova, Edward B Jude
The aim of this study was to compare the serum levels of the anti-angiogenic factor endostatin (S-endostatin) as a potential marker of vasculogenesis after autologous cell therapy (ACT) versus percutaneous transluminal angioplasty (PTA) in diabetic patients with critical limb ischemia (CLI). A total of 25 diabetic patients with CLI treated in our foot clinic during the period 2008-2014 with ACT generating potential vasculogenesis were consecutively included in the study; 14 diabetic patients with CLI who underwent PTA during the same period were included in a control group in which no vasculogenesis had occurred...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29851545/treatment-of-relapsed-myeloma-in-a-patient-with-renal-insufficiency
#8
Joan Bladé, Laura Rosiñol, María Teresa Cibeira, Carlos Fernández de Larrea
The Oncology Grand Rounds series is designed to place original reports published in the Journal into clinical context. A case presentation is followed by a description of diagnostic and management challenges, a review of the relevant literature, and a summary of the authors' suggested management approaches. The goal of this series is to help readers better understand how to apply the results of key studies, including those published in Journal of Clinical Oncology, to patients seen in their own clinical practice...
May 31, 2018: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/29848573/prospective-analysis-of-adoptive-til-therapy-in-patients-with-metastatic-melanoma-response-impact-of-anti-ctla4-and-biomarkers-to-predict-clinical-outcome
#9
Marie-Andrée Forget, Cara Haymaker, Kenneth R Hess, Yuzhong Jeff Meng, Caitlin Creasy, Tatiana V Karpinets, Orenthial J Fulbright, Jason Roszik, Scott E Woodman, Young Uk Kim, Donastas Sakellariou-Thompson, Ankit Bhatta, Arely Wahl, Esteban Flores, Shawne T Thorsen, Rene J Tavera, Renjith Ramachandran, Audrey M Gonzalez, Christopher Toth, Seth Wardell, Rahmatu Mansaray, Vruti Patel, Destiny Joy Carpio, Carol S Vaughn, Chantell M Farinas, Portia G Velasquez, Wen-Jen Hwu, Sapna P Patel, Michael A Davies, Adi Diab, Isabella C Glitza, Hussein Tawbi, Michael K K Wong, Suzanne Cain, Merrick I Ross, Jeffrey E Lee, Jeffrey E Gershenwald, Anthony Lucci, Richard Royal, J N Cormier, Jennifer A Wargo, Laszlo G Radvanyi, Carlos A Torres Cabala, Rameen Beroukhim, Patrick Hwu, Rodabe N Amaria, Chantale Bernatchez
PURPOSE: Adoptive cell therapy (ACT) using tumor-infiltrating lymphocytes (TIL) has consistently demonstrated clinical efficacy in metastatic melanoma. Recent widespread use of checkpoint blockade has shifted the treatment landscape, raising questions regarding impact of these therapies on response to TIL and appropriate immunotherapy sequence. EXPERIMENTAL DESIGN: Seventy-four metastatic melanoma patients were treated with autologous TIL and evaluated for clinical response according to irRC, overall survival and progression free survival...
May 30, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29846337/perceived-stress-and-surgical-wound-cytokine-patterns
#10
Valentina Sage Lucas, Nancy McCain, R K Elswick, Andrea L Pozez
This was a pilot study to examine pre- and postoperative stress experienced by women who were undergoing autologous breast reconstruction and how stress might impact wound healing, specifically examining cytokines and other chemical mediators in the wound environment. A nonexperimental descriptive design over time was utilized. Participants were women who were undergoing autologous abdominal breast reconstruction for breast cancer (N = 20). Data were collected preoperatively and at 24, 48, 72, and 96 hr postsurgery...
April 2018: Plastic Surgical Nursing
https://www.readbyqxmd.com/read/29806278/-in-vivo-study-of-a-novel-micro-arc-oxidation-coated-magnesium-zinc-calcium-alloy-scaffold-autologous-bone-particles-repairing-critical-size-bone-defect-in-rabbit
#11
Nan Zhang, Na Liu, Chu Sun, Jianfeng Zhu, Dongxu Wang, Yunfeng Dai, Yunfeng Wu, Yaming Wang, Junlei Li, Dewei Zhao, Jinglong Yan
Objective: To evaluate the effect of a novel micro-arc oxidation (MAO) coated magnesium-zinc-calcium (Mg-Zn-Ca) alloy scaffold/autologous bone particles to repair critical size bone defect (CSD) in rabbit and explore the novel scaffold in vivo corrosion resistance and biocompatibility. Methods: Seventy-two New Zealand white rabbits were randomly divided into 3 groups ( n =24), group A was uncoated Mg-Zn-Ca alloy scaffold group, group B was 10 μm MAO coated Mg-Zn-Ca alloy scaffold group, and group C was control group with only autologous bone graft...
March 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29803299/regenerative-medicine-and-rehabilitation-for-tendinous-and-ligamentous-injuries-in-sport-horses
#12
REVIEW
Kyla F Ortved
Tendon and ligament injuries are a common source of lameness in the athletic horse. Although tendons and ligaments have the ability to spontaneously heal, lesions tend to fill with biomechanically inferior fibrous tissue such that the horse is prone to reinjury. Regenerative medicine is used to improve quality of repair tissue and prevent reinjury. Platelet-rich plasma, stem cells, and autologous conditioned serum are the most commonly used orthobiologics in the horse. A tailored rehabilitation program is key to returning horses to athleticism following injury...
May 23, 2018: Veterinary Clinics of North America. Equine Practice
https://www.readbyqxmd.com/read/29803179/platelet-rich-plasma-stromal-vascular-fraction-and-autologous-conditioned-serum-in-treatment-of-knee-osteoarthritis
#13
REVIEW
Ali Fotouhi, Arash Maleki, Sanam Dolati, Ali Aghebati-Maleki, Leili Aghebati-Maleki
Osteoarthritis (OA) is a multifactorial chronic disease, causing several problems on patients, hygiene and community care systems. Conventional therapies, such as non-pharmacological mediations, systemic drug treatment and intra-articular therapies are applying previously; however, controlling and management approaches of the disease mainly remain insufficient. Injections of intra-articular therapies directly into the joint evade conservative obstacles to joint entry, rise bioavailability and minor systemic toxicity...
August 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29796439/various-therapies-for-ocular-surface-diseases
#14
Alina Gheorghe, Ancuţa Teodora Rosoga, Fildys Mrini, Iulia Vărgău, Florentina Gherghiceanu
This paper aims to discuss various therapeutical strategies (corneal cross-linking, amniotic membrane transplantation with or without autologous serum application, medical regenerative therapy) for treating ocular surface diseases according to medical indications, etiology, local and general status of the patient. Besides the evolution and treatment of the lesions induced by corneal foreign body, ocular burns, neurotrophic keratitis, pterygium removal, Mooren's ulcer, this paper also follows and evaluates the migration, stratification and development of corneal epithelium...
January 2018: Romanian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29784164/serum-drops-for-ocular-surface-disease-national-survey-of-canadian-cornea-specialists
#15
Michael Marchand, Mona Harissi-Dagher, Marc Germain, Paul Thompson, Marie-Claude Robert
OBJECTIVE: To review the use and preparation of serum drops for the treatment of ocular surface disease in Canada and to assess the need for an improved access to this therapy. DESIGN: Cross-sectional study. PARTICIPANTS: Cornea specialist members of the Canadian Ophthalmological Society and Canadian laboratories preparing serum tears. METHODS: A confidential survey was emailed to participants in April 2016 and continued through September 2016...
June 2018: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/29777790/effect-of-intratesticular-hematoma-on-testis-microstructure-spermatogenesis-and-testosterone-production-defining-a-cut-off-point-for-significant-intratesticular-hematoma
#16
Alireza Aminsharifi, Alireza Monsef, Ali Noorafshan, Saied Karbalay-Doust, Zahra Jafarinezhad, Omid Koohi, Afshin Molaei
OBJECTIVES: To evaluate the effect of different degrees of intratesticular hematoma (ITH), a major consequence of blunt testis trauma, on testis microstructure and function. We determined a cut-off point for significant vs. nonsignificant ITH. METHODS: Seventy male rats were divided into 7 equal groups. The control group (G) remained intact. Sham groups consisted of rats given an intratesticular injection of normal saline in both testicles with volumes equivalent to 40% of testis volume (G-II), 30% (G-III) and 10% (G-IV)...
May 16, 2018: Urology
https://www.readbyqxmd.com/read/29760728/mesenchymal-stromal-cells-cultured-in-serum-from-heart-failure-patients-are-more-resistant-to-simulated-chronic-and-acute-stress
#17
Timo Z Nazari-Shafti, Zhiyi Xu, Andreas Matthäus Bader, Georg Henke, Kristin Klose, Volkmar Falk, Christof Stamm
Despite regulatory issues surrounding the use of animal-derived cell culture supplements, most clinical cardiac cell therapy trials using mesenchymal stromal cells (MSCs) still rely on fetal bovine serum (FBS) for cell expansion before transplantation. We sought to investigate the effect of human serum from heart failure patients (HFS) on cord blood MSCs (CB-MSCs) during short-term culture under regular conditions and during simulated acute and chronic stress. Cell survival, proliferation, metabolic activity, and apoptosis were quantified, and gene expression profiles of selected apoptosis and cell cycle regulators were determined...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29755905/a-case-of-neurotrophic-keratopathy-concomitant-to-brain-metastasis
#18
Chiara Bonzano, Elisabetta Bonzano, Carlo Alberto Cutolo, Riccardo Scotto, Carlo Enrico Traverso
We report a case of a 63-year-old Caucasian female referred to the cornea service of Clinica Oculistica with a neurotrophic corneal ulcer, decreased corneal sensitivity, absent corneal reflex, and decreased lacrimation. The medical record review was relevant for mastectomy and adjuvant therapy for breast cancer complicated by pontocerebellar angle metastasis. Eye patching and application of antibiotic and vitamin ointments were prescribed at first, without a significant improvement. Thus, treatment with autologous serum was started...
March 12, 2018: Curēus
https://www.readbyqxmd.com/read/29751821/improved-gmp-compliant-approach-to-manipulate-lipoaspirates-to-cryopreserve-stromal-vascular-fraction-and-to-expand-adipose-stem-cells-in-xeno-free-media
#19
Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply with Good Manufacturing Practice (GMP), fetal bovine serum (FBS), used to expand ASC in vitro, could be replaced by growth factors from platelet concentrates. METHODS: Throughout each protocol, GMP-compliant reagents and devices were used...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29748216/intrinsic-anti-inflammatory-properties-in-the-serum-of-two-species-of-deep-diving-seal
#20
Aranya Bagchi, Annabelle J Batten, Milton Levin, Kaitlin N Allen, Michael L Fitzgerald, Luis A Hückstädt, Daniel P Costa, Emmanuel S Buys, Allyson G Hindle
Weddell and elephant seals are deep diving mammals, which rely on lung collapse to limit nitrogen absorption and prevent decompression injury. Repeated collapse and re-expansion exposes the lungs to multiple stressors, including ischemia/reperfusion, alveolar shear stress, and inflammation. There is no evidence, however, that diving damages pulmonary function in these species. To investigate potential protective strategies in deep-diving seals, we examined the inflammatory response of seal whole blood exposed to lipopolysaccharide (LPS), a potent endotoxin...
May 10, 2018: Journal of Experimental Biology
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