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https://www.readbyqxmd.com/read/29665931/-establishment-of-a-serum-free-culture-system-based-on-heparin-and-anti-cd16-antibody-for-expansion-of-human-cord-blood-nk-cells
#1
Qiao-Chu Wang, Liang Zhen
OBJECTIVE: To establish a novel method for ex vivo expansion of natural killer cells from human umbilical blood, so as to provide the basis for NK cell therapy. METHODS: Mononucleated cells from human umbilical blood were harvested and suspended in a serum-free medium containing 5% autologous plasma, recombinant human IL-15 (50 ng/ml) and hydrocortisone sodium succinate (5×10-8 mol/L) at a concentration of 1.5×106 /ml, then the cells were seeded into flasks pre-coated with heparin sodium (100 U/cm2 ) or/and anti-human CD16 antibody (1 µg/cm2 )...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29665930/-system-amplifying-nk-cells-using-mononuclear-cell-culture
#2
Shi-Jian Huang, Hong-Min Yan, Zi-Kuan Guo
OBJECTIVE: To develop an easy method to amplify natural killer (NK) cells by using mononuclear cells in vitro, so as to lay the basis for NK cell therapy. METHODS: Umbilical cord blood from 3 healthy full-term pregnant women was collected, and the peripheral blood mononuclear cells (PBMNC) were harvested by density gradient centrifugation. Each sample of PBMNC was divided into 3 groups: CD16mAb, CD3 mAb and CD16mAb+CD3mAb- groups. The culture flasks were pre-coated with CD16, CD3 or CD3 plus CD16 mAb...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29661028/development-of-large-scale-manufacturing-of-adipose-derived-stromal-cells-for-clinical-applications-using-bioreactors-and-human-platelet-lysate
#3
Mandana Haack-Sørensen, Morten Juhl, Bjarke Follin, Rebekka Harary Søndergaard, Maria Kirchhoff, Jens Kastrup, Annette Ekblond
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor)...
April 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/29656052/autologous-serum-collected-1h-post-exercise-enhances-natural-killer-cell-cytotoxicity
#4
Priti Gupta, Austin B Bigley, Melissa Markofski, Mitzi Laughlin, Emily C LaVoy
Natural Killer cells are cytotoxic lymphocytes that recognize and eliminate tumor cells. Exercise enhances NK cell cytotoxic activity (NKCA), yet the underlying mechanisms are not fully understood. Exercise-induced shifts in NK-cell subsets has been proposed as one mechanism. Alternatively, exercise alters stress hormone and cytokine levels, which are also known to affect NKCA. AIM: Determine the role(s) of exercise-induced shifts in the proportions of NK-cell subsets found in the blood, and changes in serum IL-2, IL-6, IL-12, IFN-γ, TNF-α and cortisol, on exercise-induced changes in NKCA...
April 12, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29655599/plasma-from-some-cancer-patients-inhibits-adenoviral-ad5f35-vector-transduction-of-dendritic-cells
#5
Ping Jin, Wenjing Chen, Jiaqiang Ren, Steven Chen, Lauren Wood, Yingdong Zhao, Alan Remaley, Chauha Pham, Sheena Lian, Shutong Liu, Hui Liu, Steven Highfill, Jay A Berzofsky, David F Stroncek
BACKGROUND: Pooled AB serum is often used as a media supplement for cell culture but it has the potential to transmit infectious diseases. To avoid this risk, we used autologous plasma as a media supplement for manufacturing dendritic cells (DCs) for cancer immunotherapy. We noticed inconsistencies in the DCs and investigated their nature and cause. METHODS: Adenovirus human epidural growth factor receptor 2 (adHER2/neu) DCs for 21 patients were manufactured from autologous peripheral blood monocytes that were treated with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 for 3 days, transduced with Ad5f35HER2ECTM and then treated with lipopolysaccharide and interferon (IFN)-γ for 1 day...
April 11, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29651333/a-novel-organ-preservation-solution-with-efficient-clearance-of-red-blood-cells-improves-kidney-transplantation-in-a-canine-model
#6
Sheng Wang, Kristyn Gumpper, Tao Tan, Xianzhang Luo, Hui Guo, Changsheng Ming, Hanying Jiang, Jiangguo Fang, Guang Du, Hua Zhu, Jianjie Ma, Zhishui Chen, Nianqiao Gong
Organ preservation solutions are designed to minimize organ damage during transplantation. A novel preservation solution, WMO-II, was developed to have a low viscosity and to improve microvasculature perfusion for kidneys. In an autologous canine transplantation model, kidney function and recovery were evaluated after organs were flushed and cold-stored with WMO-II or HTK solution, a perfusate currently approved for clinical use. The average number of red blood cells remaining in a single glomerulus after flushing with WMO-II was significantly reduced when compared with HTK solution...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29644723/a-french-observational-study-describing-the-use-of-human-polyvalent-immunoglobulins-in-hematological-malignancy-associated-secondary-immunodeficiency
#7
Omar Benbrahim, Jean-François Viallard, Sylvain Choquet, Bruno Royer, Frédéric Bauduer, Olivier Decaux, Jean-Charles Crave, Yann Fardini, Pierre Clerson, Vincent Levy
OBJECTIVE: To describe the characteristics of patients suffering from secondary immunodeficiencies (SID) associated with hematological malignancies (HM), who started immunoglobulins replacement therapy (IgRT), physicians' expectations regarding IgRT and IgRT modalities. METHODS: Non-interventional, prospective French cross-sectional study. RESULTS: The analysis included 231 patients (66±12 years old) suffering from multiple myeloma (MM) (N=64), chronic lymphoid leukemia (CLL) (N=84), aggressive non-Hodgkin B-cell lymphoma (aNHL) (N=32), indolent NHL (N=39), acute leukemia (N=6), Hodgkin disease (N=6)...
April 12, 2018: European Journal of Haematology
https://www.readbyqxmd.com/read/29618688/-human-herpesvirus-6-pneumonitis-following-autologous-peripheral-blood-stem-cell-transplantation
#8
Yuu Saitoh, Moritaka Gotoh, Seiichiro Yoshizawa, Daigo Akahane, Hiroaki Fujimoto, Yoshikazu Ito, Kazuma Ohyashiki
A-46-year-old man was diagnosed with peripheral T cell lymphoma, not otherwise specified. He achieved a complete remission after pirarubicin, cyclophosphamide, vincristine, and prednisolone (THP-COP) therapy and successful autologous peripheral blood stem-cell transplantation (AutoSCT). However, 6 months post AutoSCT, he complained of fever. Chest computed tomography of the patient displayed bilateral interstitial pneumonitis. Human herpesvirus-6 (HHV-6) DNA was detected in his bronchoalveolar lavage fluid...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
https://www.readbyqxmd.com/read/29611226/susceptibility-of-a-fumigatus-specific-t-cell-assays-to-pre-analytic-blood-storage-and-pbmc-cryopreservation-greatly-depends-on-readout-platform-and-analytes
#9
Chris D Lauruschkat, Sebastian Wurster, Lukas Page, Maria Lazariotou, Mariola Dragan, Philipp Weis, Andrew J Ullmann, Hermann Einsele, Jürgen Löffler
Mould specific T-cells detectable by flow cytometry or ELISPOT were proposed as a novel biomarker in invasive aspergillosis. To define protocols facilitating sample shipment and longitudinal analysis, this study evaluated the susceptibility of different functional assays for A. fumigatus specific T-cell quantification and characterisation to pre-analytic delays. PBMCs from six healthy donors were analysed after immediate isolation, after 6 hours whole blood storage or after cryopreservation using three different common media...
April 3, 2018: Mycoses
https://www.readbyqxmd.com/read/29608319/warm-autoadsorption-using-zzap
#10
Farai M Tsimba-Chitsva, Amy Caballero, Becky Svatora
The masking of clinically significant alloantibodies by warm autoantibodies presents challenges in pretransfusion testing. The adoption of transfusion practices such as the issuing of "least incompatible" red blood cells (RBCs) without a complete antibody workup is potentially unsafe for patients. Several autoadsorption methods can be used to remove autoantibody reactivity. ZZAP treatment of autologous RBCs is an efficient way to prepare the cells for autoadsorption. Autoadsorbed serum or plasma can then be used to remove autoantibody reactivity and identify clinically significant alloantibodies...
January 2018: Immunohematology
https://www.readbyqxmd.com/read/29602451/-autologous-serum-tears-long-term-treatment-in-dry-eye-syndrome
#11
M Beylerian, M Lazaro, J Magalon, J Veran, A Darque, F Grimaud, N Stolowy, H Beylerian, F Sabatier, L Hoffart
INTRODUCTION: Dry eye disease is a multifactorial pathology of the ocular surface. The high incidence of this pathology, as well as its significant impact on quality of life and vision and its financial cost, makes it a real public health problem. While the treatment of mild cases is generally simple and effective, treatment of severe forms is often disappointing. The use of autologous serum tears (AST) represents a therapeutic alternative for the most severe cases. The purpose of our study is to evaluate the efficacy of long-term AST treatment in patients with severe dry eye disease refractory to conventional treatment or secondary to systemic diseases such as Sjögren's syndrome or Graft versus Host disease (GVH), or ocular pathologies such as neurotrophic keratitis, chemical burns and ocular cicatricial pemphigoid...
March 27, 2018: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/29594489/host-genetic-susceptibility-to-clostridium-difficile-infections-in-patients-undergoing-autologous-stem-cell-transplantation-a-genome-wide-association-study
#12
Senu Apewokin, Jeannette Y Lee, Julia A Goodwin, Kent D McKelvey, Owen W Stephens, Daohong Zhou, Elizabeth Ann Coleman
BACKGROUND: Clostridium difficile infection (CDI) is the most common hospital-acquired infection. Unfortunately, genes that identify CDI-susceptible patients have not been well described. We performed a genome-wide association study (GWAS) to determine genetic variants associated with the development of CDI. METHODS: A cohort study of Caucasian patients undergoing autologous stem cell transplantation for multiple myeloma was performed. Patients were genotyped using Illumina® Whole Genome Genotyping Infinium chemistry...
March 28, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/29580865/bone-marrow-mesenchymal-stromal-cell-infusion-in-patients-with-chronic-kidney-disease-a-safety-study-with-18-months-of-follow-up
#13
Atieh Makhlough, Soroosh Shekarchian, Reza Moghadasali, Behzad Einollahi, Mona Dastgheib, Ghasem Janbabaee, Seyedeh Esmat Hosseini, Nasrin Falah, Fateme Abbasi, Hossein Baharvand, Nasser Aghdami
BACKGROUND: Chronic kidney disease (CKD) is a progressive loss of kidney function and structure that affects approximately 13% of the population worldwide. A recent meta-analysis revealed that cell-based therapies improve impaired renal function and structure in preclinical models of CKD. We assessed the safety and tolerability of bone marrow-mesenchymal stromal cell (MSC) infusion in patients with CKD. METHODS: A single-arm study was carried out at one center with 18-month follow-up in seven eligible patients with CKD due to different etiologies such as hypertension, nephrotic syndrome (NS) and unknown etiology...
March 23, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29569331/mesenchymal-stem-cell-seeded-biomimetic-3d-printed-scaffolds-induce-complete-bridging-of-femoral-critical-sized-defects
#14
John A Szivek, David A Gonzales, Andrew M Wojtanowski, Michael A Martinez, Jordan L Smith
No current clinical treatments provide an ideal long-term solution for repair of long bone segment defects. Incomplete healing prevents patients from returning to preinjury activity and ultimately requires additional surgery to induce healing. Obtaining autologous graft material is costly, incurs morbidity, requires surgical time, and quality material is finite. In this pilot study, 3D printed biomimetic scaffolds were used to facilitate rapid bone bridging in critical sized defects in a sheep model. An inverse trabecular pattern based on micro-CT scans of sheep trabecular bone was printed in polybutylene terephthalate...
March 23, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29563103/chimeric-antigen-receptor-t-cell-mediated-neurotoxicity-in-non-human-primates
#15
Agne Taraseviciute, Victor Tkachev, Rafael Ponce, Cameron J Turtle, Jessica M Snyder, H Denny Liggitt, David Myerson, Luis Gonzalez-Cuyar, Audrey Baldessari, Chris English, Alison Yu, Hengqi Zheng, Scott N Furlan, Daniel J Hunt, Virginia Hoglund, Olivia Finney, Hannah Brakke, Bruce R Blazar, Carolina Berger, Stanley Riddell, Rebecca Gardner, Leslie S Kean, Michael C Jensen
Chimeric Antigen Receptor (CAR) T cell immunotherapy has revolutionised the treatment of refractory leukemias and lymphomas, but is associated with significant toxicities, namely cytokine release syndrome (CRS) and neurotoxicity. A major barrier to developing therapeutics to prevent CAR T cell-mediated neurotoxicity is the lack of clinically relevant models. Accordingly, we developed a rhesus macaque (RM) model of neurotoxicity via adoptive transfer of autologous CD20-specific CAR T cells. Following cyclophosphamide lymphodepletion, CD20 CAR T cells expand to 272-4450 cells/µl after 7-8 days and elicit CRS and neurotoxicity...
March 21, 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29554212/combined-immunotherapy-encompassing-intratumoral-poly-iclc-dendritic-cell-vaccination-and-radiotherapy-in-advanced-cancer-patients
#16
M E Rodríguez-Ruiz, J L Perez-Gracia, I Rodríguez, C Alfaro, C Oñate, G Pérez, I Gil-Bazo, A Benito, S Inogés, A López-Diaz de Cerio, M Ponz-Sarvise, L Resano, P Berraondo, B Barbés, S Martin-Algarra, A Gúrpide, M F Sanmamed, C de Andrea, A M Salazar, I Melero
BACKGROUND: Combination immunotherapy has the potential to achieve additive or synergistic effects. Combined local injections of dsRNA analogues (mimicking viral RNA) and repeated vaccinations with tumor-lysate loaded dendritic cells shows efficacy against colon cancer mouse models. In the context of immunotherapy, radiotherapy can exert beneficial abscopal effects. PATIENTS AND METHODS: In this two-cohort pilot phase I study, 15 advanced cancer patients received two 4-week cycles of four intradermal daily doses of monocyte-derived dendritic cells preloaded with autologous tumor lysate and matured for 24h with poly-ICLC (Hiltonol), TNF-α and IFN-α...
March 14, 2018: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29528589/the-effect-of-autologous-serum-therapy-in-chronic-spontaneous-urticaria-depends-on-autologous-serum-skin-test-positivity
#17
Sahar Z Elazab, Waheed F Hessam
Chronic spontaneous urticaria (CSU) is a common distressing disease caused mainly by autoreactive mechanisms. Autologous serum therapy (AST) has been tried for treatment of many autoimmune diseases including chronic urticaria. We assessed the effect of AST in autologous serum skin test (ASST)-positive and ASST-negative CSU patients. The study included 40 patients clinically diagnosed as CSU. Twenty ASST-positive patients and 20 ASST-negative patients received AST for 8 weeks. Urticarial activity was assessed at baseline, 4th week, 9th week, and at the 12th week by the weekly urticarial activity score (UAS-7) according to the EAACI/GA2LEN/EDF/WAO guidelines...
June 2017: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/29527528/reflections-on-dry-eye-syndrome-treatment-therapeutic-role-of-blood-products
#18
REVIEW
Victor J Drew, Ching-Li Tseng, Jerard Seghatchian, Thierry Burnouf
Dry eye syndrome (DES) is a multifactorial, frequent, pathology characterized by deficient tear production or increased evaporation of tears and associated with ocular surface alteration and inflammation. It mostly affects, but not exclusively, older individuals and leads to varying degrees of discomfort and decreased quality of life. Although the typical treatments of DES rely on using artificial tears, polyunsaturated fatty acids, integrin antagonists, anti-inflammatory agents, or on performing punctal occlusion, recently, standardized blood-derived serum eye drops (SED) are generating much interest as a new physiological treatment option...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29527029/omalizumab-for-management-of-refractory-urticaria-experience-of-a-tertiary-care-centre-in-eastern-india
#19
Shekhar Neema, Manas Chatterjee
Aim: To study the effects of omalizumab in chronic spontaneous urticaria in Indian patients. Setting and Design: The study was conducted in a tertiary care centre and it was retrospective and descriptive in nature. Material and Methods: We analysed the data of patients who were administered omalizumab between June 2014 and June 2015 for the management of refractory chronic spontaneous urticaria at our centre. Omalizumab was used in those patients who did not respond to updosing of antihistaminics and cyclosporine...
January 2018: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/29517616/a-dose-escalation-study-of-recombinant-human-interleukin-18-in-combination-with-ofatumumab-after-autologous-peripheral-blood-stem-cell-transplantation-for-lymphoma
#20
Michael J Robertson, Christopher W Stamatkin, David Pelloso, Jill Weisenbach, Nagendra K Prasad, Ahmad R Safa
Interleukin-18 (IL-18) is an immunostimulatory cytokine that augments antibody-dependent cellular cytotoxicity mediated by human natural killer cells against antibody-coated lymphoma cells in vitro and that has antitumor activity in animal models. Ofatumumab is a CD20 monoclonal antibody with activity against human B-cell lymphomas. A phase I study of recombinant human (rh) IL-18 given with ofatumumab was undertaken in patients with CD20 lymphoma who had undergone high-dose chemotherapy and autologous peripheral blood stem cell transplantation...
April 2018: Journal of Immunotherapy
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