keyword
MENU ▼
Read by QxMD icon Read
search

serum autologous

keyword
https://www.readbyqxmd.com/read/28730081/effect-of-human-autologous-serum-and-fetal-bovine-serum-on-human-corneal-epithelial-cell-viability-migration-and-proliferation-in-vitro
#1
Ming-Feng Wu, Tanja Stachon, Berthold Seitz, Achim Langenbucher, Nóra Szentmáry
AIM: To analyze the concentration-dependent effects of autologous serum (AS) and fetal bovine serum (FBS) on human corneal epithelial cell (HCEC) viability, migration and proliferation. METHODS: AS was prepared from 13 patients with non-healing epithelial defects Dulbecco's modified eagle medium/Ham's F12 (DMEM/F12) with 5% FBS, 0.5% dimethyl sulphoxide (DMSO), 10 ng/mL human epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in serum media: DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS or FBS...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28726797/multiple-myeloma
#2
REVIEW
Shaji K Kumar, Vincent Rajkumar, Robert A Kyle, Mark van Duin, Pieter Sonneveld, María-Victoria Mateos, Francesca Gay, Kenneth C Anderson
Multiple myeloma is a malignancy of terminally differentiated plasma cells, and patients typically present with bone marrow infiltration of clonal plasma cells and monoclonal protein in the serum and/or urine. The diagnosis of multiple myeloma is made when clear end-organ damage attributable to the plasma cell proliferative disorder or when findings that suggest a high likelihood of their development are present. Distinguishing symptomatic multiple myeloma that requires treatment from the precursor stages of monoclonal gammopathy of undetermined significance and smouldering multiple myeloma is important, as observation is the standard for those conditions...
July 20, 2017: Nature Reviews. Disease Primers
https://www.readbyqxmd.com/read/28725315/oligoclonal-pattern-abnormal-protein-bands-in-post-treatment-plasma-cell-myeloma-patients-implications-for-protein-electrophoresis-and-serum-free-light-chain-assay-results
#3
Gurmukh Singh
BACKGROUND: The impact of autologous stem cell transplantation (ASCT) in plasma cell myeloma patients on the frequency, quality, and timing of oligoclonal pattern in serum protein electrophoresis/immunofixation electrophoresis (SPEP/SIFE) and serum free light chain assay (SFLCA) was evaluated. METHODS: Laboratory results and clinical data for 251 patients with plasma cell myeloma, who had SPEP/SIFE and/or SFLCA performed between January 2010 and December 2016 were reviewed...
August 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/28718904/autologous-serum-eye-drops-and-enhanced-epithelial-healing-time-after-photorefractive-keratectomy
#4
Hanife Tuba Akcam, Metin Unlu, Emine Esra Karaca, Hulya Yazici, Bahri Aydin, Ahmet Murad Hondur
BACKGROUND: The aim of the study is to test whether use of autologous serum eye-drops can provide earlier epithelial healing following the application of photorefractive keratectomy. METHOD: Sixty patients (60 eyes) underwent photorefractive keratectomy for myopia. Thirty eyes received autologous serum drops (Study group) while 30 eyes received conventional artificial tears (Control group) after photorefractive keratectomy. An 8 mm epithelial opening was prepared with the application of 18 per cent alcohol for 20 seconds...
July 18, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/28716826/il-10-producing-infliximab-specific-t-cells-regulate-the-antidrug-t-cell-response-in-exposed-patients
#5
Alessandra Vultaggio, Francesca Nencini, Sara Pratesi, Daniele Cammelli, Maria Totaro, Sergio Romagnani, Enrico Maggi, Andrea Matucci
Infliximab (IFX) is a chimeric mAb that can lead to the appearance of anti-drug Abs. Recent research has identified the presence of circulating IFX-specific T cells in treated patients. The aim of the study was to analyze the functional characteristics of IFX-specific T cells, in particular their capability to produce biologically active regulatory cytokines. Drug-stimulated PBMCs or coculture systems were used to detect memory T cells in treated patients. The cytokines produced by IFX-specific T cells, T cell lines, and T cell clones were evaluated at the mRNA and protein levels...
July 17, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28714496/clinical-outcome-of-autologous-serum-therapy-in-chronic-idiopathic-urticaria
#6
D Karn, S Kc
BACKGROUND: Quality of life in chronic idiopathic urticaria is hampered as efficacy of H1-antihistamines is limited. Autologous serum containing tolerance-generating anti-idiotype antibodies is a novel and cost-effective therapy. This study was conducted to evaluate the efficacy of autologous serum therapy (AST) among chronic urticaria patients with autologous skin prick test positive and negative status. METHODS: Untreated 102 patients of chronic urticaria were enrolled in a non-randomized interventional study...
January 2017: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/28682474/autologous-serum-supplement-favours-in-vitro-regenerative-paracrine-factors-synthesis
#7
Nazmul Haque, Noor Hayaty Abu Kasim, Noor Lide Abu Kassim, Mohammad Tariqur Rahman
OBJECTIVES: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. MATERIALS AND METHODS: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated...
July 6, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28665007/case-of-cholinergic-urticaria-accompanied-by-anaphylaxis
#8
Shigeruko Iijima, Kazumi Kojo, Noriko Takayama, Makiko Hiragun, Takanobu Kan, Michihiro Hide
Cholinergic urticaria occasionally occurs in combination with anaphylactic symptoms. However, this has not been widely reported. Herein, we report the case of a 14-year-old Japanese male who was diagnosed with cholinergic urticaria accompanied by anaphylaxis. The patient, who was suffering from atopic dermatitis and bronchial asthma, had developed wheals after exercising or bathing, which would have increased his core body temperature, since summer 2014. He experienced two episodes of severe systemic symptoms and wheal development when he took a bath after eating in December 2014 and the following January...
June 30, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28638679/decrease-of-serum-biomarker-of-type-ii-collagen-degradation-coll2-1-by-intra-articular-injection-of-an-autologous-plasma-rich-platelet-in-patients-with-unilateral-primary-knee-osteoarthritis
#9
Rasha Mohamed Fawzy, Nashwa Ismail Hashaad, Amira Ibrahim Mansour
OBJECTIVE: This study aimed to evaluate the effect of one dose of intra-articular injection of (PRP) in the knee joint on a specific osteoarthritis (OA) serum biomarker of cartilage degeneration, Collagen 2-1 (Coll2-1), over a short period of 3 months. The aim extended to clarify the effect of PRP on the functional status of the osteoarthritic knee joint. MATERIAL AND METHODS: Sixty patients with primary unilateral knee OA were enrolled in this study. They were subdivided according to Kellgren-Lawrence grading scale (KL) into (Group I): including patients with KL grade < 3 and (Group II): including patients with KL grade ≥3...
June 2017: European Journal of Rheumatology
https://www.readbyqxmd.com/read/28630095/antibody-dependent-nk-cell-activation-differentially-targets-ebv-infected-cells-in-lytic-cycle-and-bystander-b-lymphocytes-bound-to-viral-antigen-containing-particles
#10
María López-Montañés, Elisenda Alari-Pahissa, Jordi Sintes, José E Martínez-Rodríguez, Aura Muntasell, Miguel López-Botet
NK cells have been reported to respond against EBV-infected B cells in the lytic cycle and to control the viral infection involving IFN-γ secretion. Early reports proposed a role for NK cell Ab-dependent cellular cytotoxicity (ADCC) triggered via FcγR-IIIA (CD16) in the response to EBV. In the current study, we revisited this issue, showing that serum from EBV(+) individuals triggered vigorous NK cell degranulation and cytokine production (i.e., TNF-α and IFN-γ) against EBV-infected cells, enhancing NK cell activation...
June 19, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28620707/treatment-of-patients-with-neurotrophic-keratitis-stages-2-and-3-with-plasma-rich-in-growth-factors-prgf-endoret-eye-drops
#11
Ronald Mauricio Sanchez-Avila, Jesus Merayo-Lloves, Ana Cristina Riestra, Luis Fernandez-Vega Cueto, Eduardo Anitua, Leire Begoña, Francisco Muruzabal, Gorka Orive
PURPOSE: To provide preliminary data about efficacy and safety of plasma rich in growth factors (PRGF) eye-drops in neurotrophic keratitis (NK) and to analyze the possible influence of certain variables on treatment outcomes. METHODS: This retrospective study included patients with stages 2-3 of NK treated with PRGF eye-drops. Primary endpoint was the resolution time of corneal ulcer defect. Outcome measures including percentage of ulcer closure at 4 weeks, Ocular Surface Disease Index (OSDI), Best-Corrected Visual Acuity (BCVA) and Visual Analogue Scale (VAS) were also evaluated before and after treatment with PRGF...
June 15, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28618186/therapeutic-efficacy-of-fresh-allogeneic-mesenchymal-stem-cells-for-severe-refractory-feline-chronic-gingivostomatitis
#12
Boaz Arzi, Kaitlin C Clark, Ayswarya Sundaram, Mathieu Spriet, Frank J M Verstraete, Naomi J Walker, Megan R Loscar, Nasim Fazel, William J Murphy, Natalia Vapniarsky, Dori L Borjesson
Mesenchymal stem cells (MSCs) have potent immunomodulatory functions and are a promising therapy for immune-mediated inflammatory disorders. We previously demonstrated the efficacy of fresh, autologous, adipose-derived MSCs (ASCs) to treat feline chronic gingivostomatitis (FCGS), a chronic oral mucosal inflammatory disease similar to human oral lichen planus. Here, we investigate the use of fresh allogeneic ASCs for treatment of FCGS in seven cats. Radiolabeled ASCs were also tracked systemically. Each cat received two intravenous injections of 20 million ASCs, 1 month apart...
June 15, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28618138/expansion-of-umbilical-cord-blood-aldehyde-dehydrogenase-expressing-cells-generates-myeloid-progenitor-cells-that-stimulate-limb-revascularization
#13
David M Putman, Tyler T Cooper, Stephen E Sherman, Ayesh K Seneviratne, Mark Hewitt, Gillian I Bell, David A Hess
Uncompromised by chronic disease-related comorbidities, human umbilical cord blood (UCB) progenitor cells with high aldehyde dehydrogenase activity (ALDH(hi) cells) stimulate blood vessel regeneration after intra-muscular transplantation. However, implementation of cellular therapies using UCB ALDH(hi) cells for critical limb ischemia, the most severe form of severe peripheral artery disease, is limited by the rarity (<0.5%) of these cells. Our goal was to generate a clinically-translatable, allogeneic cell population for vessel regenerative therapies, via ex vivo expansion of UCB ALDH(hi) cells without loss of pro-angiogenic potency...
June 15, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28612572/-comparison-of-two-methods-of-lidocaine-administrating-for-neuroprotection-in-rabbit-model-of-subarachnoid-hemorrhage
#14
Xian-Qing Shi, Yong-Jian Fu, Li-Rong Zheng
OBJECTIVES: To compare the neuroprotection effect of two methods of Lidocaine administration in rabbit model of subarachnoid hemorrhage. METHODS: Forty New Zealand white rabbits were randomly divided into sham group, subarachnoid hemorrhage group (SAH), Lidocaine intravenous injection group (L1), and Lidocaine intracisternal administration group (L2). The rabbits were given general anaesthesia, then 1.5 mL autologous nonheparinized arterial blood was injected into cisterna magna to establish SAH model, while 1...
January 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28610596/complete-human-serum-maintains-viability-and-chondrogenic-potential-of-human-synovial-stem-cells-suitable-conditions-for-transplantation
#15
Mitsuru Mizuno, Hisako Katano, Koji Otabe, Keiichiro Komori, Yuji Kohno, Shizuka Fujii, Nobutake Ozeki, Masafumi Horie, Kunikazu Tsuji, Hideyuki Koga, Takeshi Muneta, Ichiro Sekiya
BACKGROUND: In our clinical practice, we perform transplantations of autologous synovial mesenchymal stem cells (MSCs) for cartilage and meniscus regenerative medicine. One of the most important issues to ensuring clinical efficacy involves the transport of synovial MSCs from the processing facility to the clinic. Complete human serum (100% human serum) is an attractive candidate material in which to suspend synovial MSCs for their preservation during transport. The purpose of this study was to investigate whether complete human serum maintained MSC viability and chondrogenic potential and to examine the optimal temperature conditions for the preservation of human synovial MSCs...
June 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28610547/human-plasma-rich-in-growth-factors-improves-survival-and-neurite-outgrowth-of-spiral-ganglion-neurons-in-vitro
#16
Michael Stolle, Jennifer Schulze, Ariane Römer, Thomas Lenarz, Martin Durisin, Athanasia Warnecke
BACKGROUND: Platelet-rich and platelet poor plasma (PRP and PPP) are autologous preparations from peripheral blood and contain several growth factors and cytokines involved in tissue repair. Although their neuroprotective and -regenerative properties have been already described, little is known about their effects in the inner ear. We therefore examined the effects of PRP and PPP on spiral ganglion neurons (SGN) in vitro. RESULTS: For all experiments, spiral ganglia were isolated from neonatal rats and were cultured in serum-free medium...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28610481/efficacy-of-humanized-mesenchymal-stem-cell-cultures-for-bone-tissue-engineering-a-systematic-review-with-a-focus-on-platelet-derivatives
#17
Siddharth Shanbhag, Andreas Stavropoulos, Salwa Suliman, Tor Hervig, Kamal Mustafa
Fetal bovine serum (FBS) is the most commonly used supplement for ex vivo expansion of human mesenchymal stem cells (hMSCs) for bone tissue engineering applications. However, from a clinical standpoint, it is important to substitute animal-derived products according to current Good Manufacturing Practice (cGMP) guidelines. Humanized alternatives to FBS include three categories of products: human serum (HS), human platelet-derivatives (HPD) - including platelet lysate (PL) or releasate (PR), produced by freeze-/thawing or chemical activation of platelet concentrates, respectively; and chemically-defined media (CDM)...
June 13, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28603071/evaluation-of-performance-status-and-hematopoietic-cell-transplantation-specific-comorbidity-index-on-unplanned-admission-rates-in-patients-with-multiple-myeloma-undergoing-outpatient-autologous-stem-cell-transplantation
#18
Cynthia Obiozor, Dipti P Subramaniam, Clint Divine, Leyla Shune, Anurag K Singh, Tara L Lin, Sunil Abhyankar, G John Chen, Joseph McGuirk, Sid Ganguly
Although outpatient autologous stem cell transplantation (ASCT) is safe and feasible in most instances, some patients undergoing planned outpatient transplantation for multiple myeloma (MM) will need inpatient admission for transplantation-related complications. We aim to evaluate the difference, if any, between outpatient and inpatient ASCT cohorts of MM patients in terms of admission rate, transplantation outcome, and overall survival. We also plan to assess whether the Hematopoietic Cell Transplantation Comorbidity Index (HCT-CI) and Karnofsky Performance Status (KPS) can predict unplanned admissions after adjusting for confounding factors...
June 8, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/28592540/covalent-linkage-of-hiv-1-trimers-to-synthetic-liposomes-elicits-improved-b-cell-and-antibody-responses
#19
Shridhar Bale, Geraldine Goebrecht, Armando Stano, Richard Wilson, Takayuki Ota, Karen Tran, Jidnyasa Ingale, Michael B Zwick, Richard T Wyatt
We have demonstrated that a liposomal array of well-ordered trimers enhances B cell activation, germinal center formation and the elicitation of tier-2 autologous neutralizing antibodies. Previously, we coupled well-ordered cleavage-independent NFL trimer via their C-terminal poly-histidine tails to nickel-lipids integrated into the lipid bilayer. Despite favorable in vivo effects, concern remains over the potentially longer term in vivo instability of non-covalent linkage of the trimers to the liposomes. Accordingly, we tested both cobalt coupling and covalent linkage of the trimers to the liposomes by reengineering the poly-histidine tail to include a free cysteine on each protomer of model BG505 NFL trimers to allow covalent linkage...
June 7, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28588689/safety-of-neural-stem-cell-transplantation-in-patients-with-severe-traumatic-brain-injury
#20
Zhigang Wang, Yong Luo, Lvan Chen, Wu Liang
Neural stem cell (NSC) therapy is a promising treatment for traumatic brain injury (TBI). In addition, mesenchymal stem cells (MSCs) have been investigated for the treatment of TBI due to their functions in neural regeneration and their neurotrophic effect. In the present study, the safety, feasibility and biological effects of autologous MSC-derived NSC-like cell transplantation were investigated in 10 patients with severe TBI. All patients received intravenous or intrathecal injections of human NSC-like cells and were evaluated with physical and neurological examinations, routine laboratory tests and neuroradiological findings...
June 2017: Experimental and Therapeutic Medicine
keyword
keyword
40218
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"