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https://www.readbyqxmd.com/read/27462899/group-a-rotavirus-gastroenteritis-post-vaccine-era-genotypes-and-zoonotic-transmission
#1
Adriana Luchs, Maria do Carmo Sampaio Tavares Timenetsky
ABSTRACTThis article provides a review of immunity, diagnosis, and clinical aspects of rotavirus disease. It also informs about the changes in epidemiology of diarrheal disease and genetic diversity of circulating group A rotavirus strains following the introduction of vaccines. Group A rotavirus is the major pathogen causing gastroenteritis in animals. Its segmented RNA genome can lead to the emergence of new or unusual strains in human populations via interspecies transmission and/or reassortment events.RESUMOEste artigo fornece uma revisão sobre imunidade, diagnóstico e aspectos clínicos da doença causada por rotavírus...
April 2016: Einstein
https://www.readbyqxmd.com/read/27393952/vitrification-based-cryopreservation-of-protocorm-like-bodies-of-an-endangered-lady-s-slipper-orchid-paphiopedilum-niveum-rchb-f-stein
#2
Supornchai Chaireok, Kanchit Thammasiri, Upatham Meesawat
BACKGROUND: Paphiopedilum niveum (Rchb.f.) Stein, an endangered lady's slipper orchid, is listed in CITES Appendix I and thus requires conserving. OBJECTIVE: The study aimed to cryopreserve protocorm-like bodies (PLBs) by a vitrification technique. MATERIALS AND METHODS: Four-month-old PLBs were precultured in modified Vacin and Went liquid medium with various sucrose concentrations for 24 h and 5 d with daily increasing sucrose concentrations followed by different exposure times to plant vitrification solution 2 (PVS2)...
May 2016: Cryo Letters
https://www.readbyqxmd.com/read/27384205/hepatitis-b-vaccination-coverage-in-patients-with-diabetes-mellitus
#3
Clarissa Cordeiro Alves Arrelias, Fernando Bellissimo-Rodrigues, Letícia Cristina Lourenço de Lima, Anderson Soares da Silva, Nereida Kilza da Costa Lima, Maria Lucia Zanetti
OBJECTIVE: Analyze the factors associated with full hepatitis B vaccination (three doses) in patients with diabetes mellitus. METHOD: Cross-sectional study, conducted in a health unit in a city in the state of São Paulo, with 255 patients on outpatient follow-up, in 2014. Data were obtained from the computerized system of the Municipal Health Department and via a structured questionnaire. A logistic regression model was used for analysis. RESULTS: Full hepatitis B vaccination was noted in 13...
April 2016: Revista da Escola de Enfermagem da U S P
https://www.readbyqxmd.com/read/25853304/industry-update
#4
Oliver C Steinbach
The present industry update covers the period 1-31 December 2014, with information sourced from company press releases, regulatory and patent agencies as well as the scientific literature. December is traditionally the month of last year-closure announcements or starting looking back into the recent past. The financing community for biotech seems on strong recovery as indicated by reports, but also in view of many deals discussed in these proceedings. The teaming up of a company owning a (novel) active ingredient and another one having a drug-delivery device addressing certain limitations proves to be a recipe for success (Asante, Unilife, Catalent and Endo Pharmaceuticals)...
April 2015: Therapeutic Delivery
https://www.readbyqxmd.com/read/24977213/agrobacterium-mediated-transformation-of-the-recalcitrant-vanda-kasem-s-delight-orchid-with-higher-efficiency
#5
Pavallekoodi Gnasekaran, Jessica Jeyanthi James Antony, Jasim Uddain, Sreeramanan Subramaniam
The presented study established Agrobacterium-mediated genetic transformation using protocorm-like bodies (PLBs) for the production of transgenic Vanda Kasem's Delight Tom Boykin (VKD) orchid. Several parameters such as PLB size, immersion period, level of wounding, Agrobacterium density, cocultivation period, and concentration of acetosyringone were tested and quantified using gusA gene expression to optimize the efficiency of Agrobacterium-mediated genetic transformation of VKD's PLBs. Based on the results, 3-4 mm PLBs wounded by scalpel and immersed for 30 minutes in Agrobacterium suspension of 0...
2014: TheScientificWorldJournal
https://www.readbyqxmd.com/read/24185522/an-in-vitro-method-for-rapid-regeneration-of-a-monopodial-orchid-hybrid-aranda-deborah-using-thin-section-culture
#6
P Lakshmanan, C S Loh, C J Goh
A thin section culture system for rapid regeneration of the monopodial orchid hybrid Aranda Deborah has been developed. Thin sections (0.6-0.7mm thick) obtained by transverse sectioning of a single shoot tip (6-7mm), when cultured in Vacin and Went medium enriched with coconut water (20% v/v), produced an average 13.6 protocorm-like bodies (PLB) after 45 days, compared to 2.7 PLB formed by a single 6-7 mm long shoot tip under same culture condition. Addition of α-naphthaleneacetic acid to Vacin and Went medium enriched with coconut water further increased PLB production by thin sections...
May 1995: Plant Cell Reports
https://www.readbyqxmd.com/read/23814439/asymbiotic-seed-germination-and-in-vitro-conservation-of-coelogyne-nervosa-a-rich-an-endemic-orchid-to-western-ghats
#7
Sonia Abraham, Jomy Augustine, T Dennis Thomas
Coelogyne nervosa is an epiphytic orchid endemic to Western Ghats, South India. The mature seeds of C. nervosa were cultured on ½ MS (Murashige and Skoog), MS, Kn (Knudson) and VW (Vacin and Went) media to evaluate the seed germination response. Of the four basal media used, MS medium supported maximum seed germination. Further experiments to enhance seed germination were done on MS medium supplemented with various concentrations (10, 20, 30 and 40 %) of coconut water (CW). Thirty percent CW gave the highest response in terms of percent seed germination (96), fresh weight (7...
July 2012: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/23459815/-coverage-of-the-basic-immunization-schedule-in-the-first-year-of-life-in-state-capitals-in-northeast-brazil
#8
Lorena Lauren Chaves Queiroz, Silvio Gomes Monteiro, Elba Gomide Mochel, Maria Amélia de Sousa Mascena Veras, Francisca Georgina Macêdo de Sousa, Márcio Lee de Meneses Bezerra, Maria Bethânia da Costa Chein
Vaccination coverage in the so-called "developing countries" is still lower than expected. Such coverage is an important indicator of population health and the quality of care provided by the health care system. The current study describes the results of a household survey to estimate coverage of the basic immunization schedule in the first year of life in State capitals in Northeast Brazil, for the 2005 birth cohort. The methodology used was that recommended by the Pan American Health Organization for surveys on vaccination coverage...
February 2013: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/22997547/a-simple-and-efficient-protocol-for-the-mass-propagation-of-cymbidium-mastersii-an-ornamental-orchid-of-northeast-india
#9
Padmaja Mohanty, Sumi Paul, Meera C Das, Suman Kumaria, Pramod Tandon
BACKGROUND AND AIMS: Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for mass multiplication may be more appropriate. The objective of this study was to develop an efficient protocol...
2012: AoB Plants
https://www.readbyqxmd.com/read/22229313/immunotherapy-for-drug-abuse
#10
REVIEW
Xiaoyun Shen, Thomas R Kosten
Substance use disorders continue to be major medical and social problems worldwide. Current medications for substance use disorders have many limitations such as cost, availability, medication compliance, dependence, diversion of some to illicit use and relapse to addiction after discontinuing their use. Immunotherapies using either passive monoclonal antibodies or active vaccines have distinctly different mechanisms and therapeutic utility from small molecule approaches to treatment. They have great potential to help the patient achieve and sustain abstinence and have fewer of the above limitations...
December 2011: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/22048121/advances-and-challenges-towards-a-vaccine-against-chagas-disease
#11
REVIEW
Israel Quijano-Hernandez, Eric Dumonteil
Chagas disease is major public health problem, affecting nearly 10 million people, characterized by cardiac alterations leading to congestive heart failure and death of 20-40% of the patients infected with Trypanosoma cruzi, the protozoan parasite responsible for the disease. A vaccine would be key to improve disease control and we review here the recent advances and challenges of a T. cruzi vaccine. There is a growing consensus that a protective immune response requires the activation of a Th1 immune profile, with the stimulation of CD8 (+) T cells...
November 2011: Human Vaccines
https://www.readbyqxmd.com/read/21796864/seroprevalence-of-hepatitis-b-virus-markers-in-individuals-for-physical-examination-in-west-china-hospital-china
#12
Y B Wang, E Q Chen, Y L Cui, L Zeng, Y J Wang, H Tang
BACKGROUND: Hepatitis B virus (HBV) is the major cause of chronic hepatitis,cirrhosis, and hepatocellular carcinoma. The global epidemiological scenario of HBV infection has been changing rapidly over the last two decades due to an effective immunization programme initiated by the World Health Organization. The objective of this study is to estimate the prevalence of HBV in apparently adult people who were taken health examination in our Hospital. METHODS: A cross-sectional seroprevalence analysis of hepatitis B virus infection was performed in 12037 adult residents (aged > or =18 years) in Chengdu, who visited Health Examination Center of West China Hospital of Sichuan University for routine medical check-up during the period from March to December 2008...
June 2011: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/21625960/dna-immunization-against-amyloid-beta-42-has-high-potential-as-safe-therapy-for-alzheimer-s-disease-as-it-diminishes-antigen-specific-th1-and-th17-cell-proliferation
#13
Doris Lambracht-Washington, Bao-Xi Qu, Min Fu, Larry D Anderson, Olaf Stüve, Todd N Eagar, Roger N Rosenberg
The pathogenesis of Alzheimer's disease (AD) has been strongly associated with the accumulation of amyloid beta (Aβ) peptides in brain, and immunotherapy targeting Aβ provides potential for AD prevention. A clinical trial in which AD patients were immunized with Aβ42 peptide was stopped when 6% of participants showed meningoencephalitis, apparently due to an inflammatory Th1 immune response. Previously, we and other have shown that Aβ42 DNA vaccination via gene gun generates a Th2 cellular immune response, which was shown by analyses of the respective antibody isotype profiles...
August 2011: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/21162617/menveo%C3%A2-a-novel-quadrivalent-meningococcal-crm197-conjugate-vaccine-against-serogroups-a-c-w-135-and-y
#14
REVIEW
Brian Cooper, Lisa DeTora, Jeffrey Stoddard
Meningococcal disease remains a global public health concern despite the wide availability of polysaccharide and polysaccharide-protein conjugate vaccines. The latter often afford a greater duration and scope of protection compared with the former. A novel quadrivalent meningococcal conjugate vaccine, Menveo(®) (MenACWY-CRM; Novartis Vacines, Bellaria-Rosia, Italy) has recently been licensed in Europe, the USA, Canada and other countries to protect adolescents and adults against disease caused by serogroups A, C, W-135 and Y...
January 2011: Expert Review of Vaccines
https://www.readbyqxmd.com/read/20094885/in-vitro-growth-and-single-leaf-photosynthetic-response-of-cymbidium-plantlets-to-super-elevated-co2-under-cold-cathode-fluorescent-lamps
#15
Atsushi Norikane, Takejiro Takamura, Masahiro Morokuma, Michio Tanaka
To examine the effectiveness of super-elevated (10,000 micromol mol(-1)) CO(2) enrichment under cold cathode fluorescent lamps (CCFL) for the clonal propagation of Cymbidium, plantlets were cultured on modified Vacin and Went (VW) medium under 0, 3,000 and 10,000 micromol mol(-1) CO(2) enrichment and two levels of photosynthetic photon flux density (PPFD, 45 and 75 micromol m(-2) s(-1)). Under high PPFD, 10,000 micromol mol(-1) CO(2) increased root dry weight and promoted shoot growth. In addition, a decrease in photosynthetic capacity and chlorosis at leaf tips were observed...
March 2010: Plant Cell Reports
https://www.readbyqxmd.com/read/18757881/asymbiotic-germination-response-to-photoperiod-and-nutritional-media-in-six-populations-of-calopogon-tuberosus-var-tuberosus-orchidaceae-evidence-for-ecotypic-differentiation
#16
Philip J Kauth, Michael E Kane, Wagner A Vendrame, Carrie Reinhardt-Adams
BACKGROUND AND AIMS: Ecotypic differentiation has been explored in numerous plant species, but has been largely ignored in the Orchidaceae. Applying a specific germination protocol for widespread seed sources may be unreliable due to inherent physiological or genetic differences in localized populations. It is crucial to determine whether ecotypic differentiation exists for restoration and conservation programmes. Calopogon tuberosus var. tuberosus, a widespread terrestrial orchid of eastern North America, is a model species to explore ecotypic differences in germination requirements, as this species occupies diverse habitats spanning a wide geographical range...
November 2008: Annals of Botany
https://www.readbyqxmd.com/read/18754065/cryopreservation-of-vanda-coerulea-protocorms-by-encapsulation-dehydration
#17
Nipawan Jitsopakul, Kanchit Thammasiri, Keiko Ishikawa
Protocorms of Vanda coerulea were successfully cryopreserved by encapsulation-dehydration in combination with a loading solution. Protocorms were selected 70 days after sowing seeds harvested from 7-month-old fruits. After encapsulation in an alginate matrix composed of 2 percent Na-alginate, 2 M glycerol plus 0.4 M sucrose (loading solution), the protocorms were precultured in modified Vacin and Went (1949) (VW) liquid medium supplemented with 0.7 M sucrose on a shaker (110 rpm) at 25 +/- 3 degree C for 20 h...
May 2008: Cryo Letters
https://www.readbyqxmd.com/read/18638293/levamisole-synergizes-proliferation-of-intestinal-iga-cells-in-weaned-pigs-immunized-with-vaccine-candidate-f4ac-nonenterotoxigenic-escherichia-coli-strain
#18
A K Janjatović, G Lacković, F Bozić, M Popović, I Valpotić
Levamisole (2, 3, 5, 6-tetrahydro-6-phenylimidazole 2,1-b thiazole) is a well-known nonspecific stimulator of host defence mechanisms. In previous investigations, we have found that levamisole acts on cell-mediated immunity in challenge-induced porcine postweaning colibacillosis (PWC). We assume that levamisole could also act synergistically on humoural immune response when applied as an adjuvant with vaccine candidate strains for oral immunization of weaned pigs against PWC. The influence of levamisole in combination with experimental F4ac(+) nonenterotoxigenic Escherichia coli (non-ETEC) vacinal strain on proliferation of IgA(+) cells was examined in 4-week-old weaned pigs experimentally infected with ETEC...
August 2008: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/18041530/-analysis-of-after-vacination-side-effects-occurred-in-teresina
#19
MULTICENTER STUDY
Telma Maria Evangelista de Araújo, Patrícia Maria Gomes de Carvalho, Raimunda Damasceno Ferreira Vieira
This study focuses the profile of the adverse events after-vaccine occurrences in Teresina in 2006. The data had been collected by means of the form application with 73 participants in 18 Basic Units of Health. The results show that the vaccines that had more produced events adverse had been tetravalente, BCG and DPT The events most frequent had been: fever, hiporresponsivo hipotonic episode, moderate irritability and local manifestations. Amongst the behaviors adopted for the health professionals, 80% had been approximately adjusted...
July 2007: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/17966485/-evaluation-of-the-vaccine-potentiality-of-2-strains-from-leptospira-interrogans-serogroup-ballum
#20
Andrés González Rodríguez, Yoandra Rodríguez Jiménez, Niurka Batista Santiesteban, Yolanda Valdés Abreu, Juan Francisco Núñez Osenes, Marta González González
Two vacine candidate strains from Ballum serogroup were characterized and their immunogenicity and protective power were studied in hamster. The monovalent vaccine formulations obtained showed a high immunogenicity and capacity of homologous protection and, in a lesser degree, of cross protection.
January 2005: Revista Cubana de Medicina Tropical
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