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Hydrogen peroxide gel

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https://www.readbyqxmd.com/read/28642912/influence-of-10-sodium-ascorbate-gel-application-time-on-composite-bond-strength-to-bleached-enamel
#1
Cintia Gaio Murad, Suellen Nunes de Andrade, Lucio Ramos Disconzi, Eliseu Aldrighi Munchow, Evandro Piva, Renata Correa Pascotto, Sandra Kiss Moura
Objective To analyze bond strength to bleached enamel following application of 10% sodium ascorbate gel. Material and methods Forty third molars were allocated into five groups (n = 8): GP - unbleached specimens restored with composite resin; GN - specimens restored immediately after bleaching; and G15; G30 and G60 (test groups) - bleached specimens treated with 10% sodium ascorbate gel for 15, 30 and 60 min before restoration. The teeth were sectioned and the buccal and lingual faces were restored. After storage in distilled water (37 °C/24 h), sticks of ±0...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28573728/substitution-of-modified-starch-with-hydrogen-peroxide-modified-rice-bran-in-salad-dressing-formulation-physicochemical-texture-rheological-and-sensory-properties
#2
Bahareh Maani, Mazdak Alimi, Shirin Shokoohi, Fatemeh Fazeli
Rice bran samples were treated under different conditions including hydrogen peroxide content (1, 4, and 7 wt%) and media pH (10.5, 11.5, and 12.5). Water holding capacity and color measurement results showed acceptable improvements compared with the untreated native bran confirmed by Fourier transform infrared analysis. Optimization of modification conditions upon characterization results suggested the introduction of 7% hydrogen peroxide at pH = 12.5. Accordingly, 1, 2 and 3 wt% of the rice bran treated under the optimized conditions, was used in salad dressing formulation; as for ...
June 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/28549437/p-p-ddt-induces-testicular-oxidative-stress-induced-apoptosis-in-adult-rats
#3
Neila Marouani, Dorsaf Hallegue, Mohsen Sakly, Moncef Benkhalifa, Khémais Ben Rhouma, Olfa Tebourbi
BACKGROUND: The 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p'-DDT) is a known persistent organic pollutant and male reproductive toxicant. The present study is designed to test the hypothesis that oxidative stress mediates p,p'-DDT-induced apoptosis in testis. METHODS: Male Wistar rats received an intraperitoneal (ip) injection of the pesticide at doses of 50 and 100mg/kg for 10 consecutive days. The oxidative stress was evaluated by biomarkers such lipid peroxidation (LPO) and metallothioneins (MTs) levels...
May 26, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28530597/dna-agarose-gel-electrophoresis-for-antioxidant-analysis-development-of-a-quantitative-approach-for-phenolic-extracts
#4
Sara Silva, Eduardo M Costa, Sandra Vicente, Mariana Veiga, Conceição Calhau, Rui M Morais, Manuela E Pintado
Most of the fast in vitro assays proposed to determine the antioxidant capacity of a compound/extract lack either biological context or employ complex protocols. Therefore, the present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a quantitative estimation of the antioxidant capacity of pure phenolic compounds as well as of a phenolic rich extract, while also considering their possible pro-oxidant effects. The result obtained demonstrated that the proposed method allowed for the evaluation of the protection of DNA oxidation [in the presence of hydrogen peroxide (H2O2) and an H2O2/iron (III) chloride (FeCl3) systems] as well as for the observation of pro-oxidant activities, with the measurements registering interclass correlation coefficients above 0...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28502737/gel-free-label-free-proteomic-photosynthetic-and-biochemical-analysis-of-cowpea-vigna-unguiculata-l-walp-resistance-against-cowpea-severe-mosaic-virus-cpsmv
#5
Anna Lidia N Varela, Setsuko Komatsu, Xin Wang, Rodolpho G G Silva, Pedro Filho N Souza, Ana Karla M Lobo, Ilka M Vasconcelos, Joaquim A G Silveira, Jose T A Oliveira
Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoã and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6days post-inoculation (DPI). Data support the assumptions that increases in biochemical (high hydrogen peroxide, antioxidant enzymes, and secondary compounds) and physiological responses (high photosynthesis index and chlorophyll content), confirmed by label-free comparative proteomic approach, in which quantitative changes in proteasome proteins, proteins related to photosynthesis, redox homeostasis, regulation factors/RNA processing proteins were observed may be implicated in the resistance of BRS-Marataoã to CPSMV...
May 11, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28500951/hydrogen-peroxide-induces-cell-proliferation-and-apoptosis-in-pulp-of-rats-after-dental-bleaching-in-vivo-effects-of-the-dental-bleaching-in-pulp
#6
Francine Benetti, João Eduardo Gomes-Filho, Luciana Louzada Ferreira, Edilson Ervolino, André Luiz Fraga Briso, Gustavo Sivieri-Araújo, Eloi Dezan-Júnior, Luciano Tavares Angelo Cintra
OBJECTIVE: This study provides an in vivo evaluation of the inflammatory response, levels of cell proliferation and apoptosis, and the presence of necrosis after dental bleaching with two concentrations of hydrogen peroxide (H2O2). DESIGN: Wistar rats were divided into Control (placebo gel), BLUE (20% H2O2, 1×50min), and MAXX (35% H2O2, 3×15min) groups. At 2 and 30days, the rats were killed (n=10). The jaws were processed for histology analysis and PCNA and Caspase-3-cleaved immunohistochemistry, and data were submitted to the Mann-Whitney or ANOVA test (P<0...
April 22, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28482661/insights-into-formation-and-structure-of-a%C3%AE-oligomers-cross-linked-via-tyrosines
#7
Shuting Zhang, Dillion M Fox, Brigita Urbanc
Alzheimer's disease (AD) pathology is hypothesized to be triggered by amyloid β-protein (Aβ) assembly into oligomers. Oligomer size distributions of both predominant Aβ alloforms, Aβ40 and Aβ42, can be determined in vitro using cross-linking followed by gel electrophoresis. Cross-linking, which can occur in vivo in the presence of copper and hydrogen peroxide, was recently shown to stabilize Aβ oligomers by inhibiting their conversion into fibrils. Whereas several studies showed that cross-linking is facilitated by dityrosine bond formation, the molecular-level mechanism of cross-linking remains unclear...
May 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28475971/novel-mononuclear-cu-ii-terpyridine-complexes-impact-of-fused-ring%C3%A2-thiophene-and-thiazole-head-groups-towards-dna-bsa-interaction-%C3%A2-cleavage-and-antiproliferative-activity-on-hepg2-and%C3%A2-triple%C3%A2-negative-cal-51%C3%A2-cell-line
#8
V M Manikandamathavan, M Thangaraj, T Weyhermuller, R P Parameswari, V Punitha, N Narasimha Murthy, Balachandran Unni Nair
Two mononuclear copper (II) terpyridine complexes namely, [Cu(Btptpy) (ClO4)](ClO4) 1, and [Cu(Bttpy) (ClO4)](ClO4) 2, (Btptpy (L1) = 4'-(Benzothiophene)-2,2':6',2″-terpyridine, Bttpy (L2) = 4'-(Benzylthiazolyl)-2,2':6',2″-terpyridine) have been synthesized and characterized. Single crystal X-ray diffraction shows that, both ligands belong to monoclinic crystal system with space group P21/c (L1) and P21/n (L2). Absorption spectral titration, DNA melting study, circular dichroism and viscosity measurement reveal that, complex 1 and 2 bind with DNA through intercalation...
April 15, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28475686/quercitrin-and-quercetin-3-%C3%AE-d-glucoside-as-chemical-chaperones-for-the-a4v-sod1-als-causing-mutant
#9
Philbert Ip, Priya Roy Sharda, Anna Cunningham, Sumon Chakrabartty, Vijay Pande, Avijit Chakrabartty
In many cases of familial amyotrophic lateral sclerosis (ALS), mutant forms of the Cu,Zn superoxide dismutase protein (SOD1) misfold and aggregate in motor neurons. Monomers of the normally homodimeric SOD1 have been found in patient tissue, presymptomatic mouse models of ALS, and in vitro misfolding assays which suggests that monomerization might be an early step in the pathological SOD1 misfolding pathway. In this study, we targeted the dimer interface with small molecules that might act as chemical chaperones to stabilize the native dimer and prevent downstream misfolding and aggregation...
May 5, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28467262/comparison-of-effectiveness-and-sensitivity-using-two-in-office-bleaching-protocols-for-a-6-hydrogen-peroxide-gel-in-a-randomized-clinical-trial
#10
P Vildósola, F Vera, J Ramírez, J Rencoret, H Pretel, O B Oliveira, M Tonetto, J Martín, E Fernández
OBJECTIVE: The aim of this blinded and randomized clinical trial was to compare two application protocols (one 36-minute application vs three 12-minute applications). We then assessed the effectiveness of the bleaching and any increase in sensitivity that was induced by bleaching via a split-mouth design. METHODS AND MATERIALS: Thirty patients were treated. One group had a half arch of teeth treated with a traditional application protocol (group A: 3 × 12 minutes for two sessions)...
May 2017: Operative Dentistry
https://www.readbyqxmd.com/read/28429928/a-dual-enzyme-hydrogen-peroxide-generation-machinery-in-hydrogels-supports-antimicrobial-wound-treatment
#11
Daniela Huber, Gregor Tegl, Anna Mensah, Bianca Beer, Martina Baumann, Nicole Borth, Christoph Sygmund, Roland Ludwig, Georg M Guebitz
The aging population and accompanying diseases like diabetes resulted in an increased occurrence of chronic wounds. Topical wound treatment with antimicrobial agents to inhibit bacterial invasion and promote wound healing is often associated with difficulties. Here, we investigated the potential of succinyl chitosan (SC)-carboxymethyl cellulose (CMC) hydrogels which constantly release clinically relevant levels of hydrogen peroxide (H2O2). CMC hydrogel matrix was in situ converted by limited hydrolysis by a cellulase into substrates accepted by cellobiose dehydrogenase (CDH) for continuous production of H2O2 (30 μM over 24 h)...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28364781/methyl-thiophanate-induced-toxicity-in-liver-and-kidney-of-adult-rats-a-biochemical-molecular-and-histopathological-approach
#12
A Feki, H Ben Saad, I Jaballi, C Magne, O Boudawara, K M Zeghal, A Hakim, Y Ben Ali, I Ben Amara
The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters were substantiated by histological and molecular data...
February 28, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28355362/controlled-clinical-trial-addressing-teeth-whitening-with-hydrogen-peroxide-in-adolescents-a-12-month-follow-up
#13
Marcelo Mendes Pinto, Marcela Leticia Leal Gonçalves, Ana Carolina Costa da Mota, Alessandro Melo Deana, Silvia Regina Olivan, Carolina Bortoletto, Camila Haddad Leal de Godoy, Katia Lumi Tanikawa Vergilio, Olga Maria Altavista, Lara J Motta, Sandra Kalil Bussadori
OBJECTIVES: To evaluate the colorimetric change in incisors and canines of adolescents aged 12 to 20 years submitted to at-home whitening and to evaluate satisfaction, sensitivity and discomfort during the procedures through a questionnaire. METHOD: Thirty adolescents were randomly assigned to the following groups: 1) 6.0% hydrogen peroxide (White Class with calcium - FGM); 2) 7.5% hydrogen peroxide (White Class with calcium - FGM); 3) 10% hydrogen peroxide (Oral B 3D White - Oral-B); 4) Control group - placebo...
March 2017: Clinics
https://www.readbyqxmd.com/read/28336378/optimized-biodegradable-polymeric-reservoir-mediated-local-and-sustained-co-delivery-of-dendritic-cells-and-oncolytic-adenovirus-co-expressing-il-12-and-gm-csf-for-cancer-immunotherapy
#14
Eonju Oh, Jung-Eun Oh, JinWoo Hong, YoonHo Chung, Yunki Lee, Ki Dong Park, Sungwan Kim, Chae-Ok Yun
Administration of dendritic cells (DCs) combined with oncolytic adenovirus (Ad) expressing antitumor cytokines induces a potent antitumor effect and antitumor immunity by ameliorating the immunosuppressive tumor microenvironment. However, this combination therapy has significant limitations due to rapid dissemination and inactivation of the therapeutics at the tumor site, necessitating multiple injections of both therapeutics. To overcome these limitations, we have utilized gelatin-based hydrogel to co-deliver oncolytic Ad co-expressing interleukin (IL)-12 and granulocyte-macrophage colony-stimulating factor (GM-CSF) (oAd) and DCs for sustained release of both therapeutics...
March 20, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28330261/purification-and-characterization-of-laccase-from-marasmius-species-bbkav79-and-effective-decolorization-of-selected-textile-dyes
#15
A B Vantamuri, B B Kaliwal
A novel laccase-producing white-rot fungus, Marasmius sp. BBKAV79 (Genbank Accession Number-KP455496, KP455497), was isolated and subjected to purification, characterization and dye decolorization study. The purified enzyme was obtained with a specific activity of 0.226 U mg(-1) protein and a final yield of 13.5 %. The enzyme was found to be a monomeric protein with a molecular mass of ~75 kDa as estimated by non-denaturing polyacrylamide gel electrophoresis (PAGE) and further confirmed with zymogram analysis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28303471/effect-of-acidity-of-in-office-bleaching-gels-on-tooth-sensitivity-and-whitening-a-two-center-double-blind-randomized-clinical-trial
#16
A D Loguercio, F Servat, R Stanislawczuk, A Mena-Serrano, M Rezende, M V Prieto, V Cereño, M F Rojas, K Ortega, E Fernandez, A Reis
OBJECTIVES: The study aimed to compare the tooth sensitivity (TS) and bleaching efficacy of two hydrogen peroxide gels with different pHs (acid pH [Pola Office, SDI] and the neutral pH [Pola Office+, SDI]) used for in-office bleaching. MATERIALS AND METHODS: Fifty-four patients from Brazil and Chile, with right superior incisor darker than A2, were selected for this double-blind, split-mouth randomized trial. Teeth were bleached in two sessions, with 1-week interval...
March 17, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28285876/one-pot-synthesis-of-a-new-2-substituted-1-2-3-triazole-1-oxide-derivative-from-dipyridyl-ketone-and-isonitrosoacetophenone-hydrazone-nickel-ii-complex-dna-binding-and-cleavage-properties
#17
Ramazan Gup, Oktay Erer, Nefise Dilek
An efficient and simple one-pot synthesis of a new 1,2,3-triazole-1-oxide via reaction between isonitrosoacetophenone hydrazone and dipyridyl ketone in the EtOH/AcOH at room temperature has been developed smoothly in high yield. The reaction proceeds via metal salt free, in-situ formation of asymmetric azine followed by cyclization to provide 1,2,3-triazole 1-oxide compound. It has been structurally characterized. The 1:1 ratio reaction of the 1,2,3-triazole 1-oxide ligand with nickel(II) chloride gives the mononuclear complex [Ni(L)(DMF)Cl2], hexa-coordinated within an octahedral geometry...
April 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28278191/effect-of-tooth-whitening-strips-on-fatigue-resistance-and-flexural-strength-of-bovine-dentin-in-vitro
#18
Laura E Tam, Namhee Kim, Grace M De Souza
OBJECTIVE: To determine the effects of whitening strips on bovine dentin fatigue resistance and flexural strength in vitro. MATERIALS AND METHODS: A total of eighty bovine dentin specimens (2x2x17mm) were treated with either: control glycerine gel on plastic film wrap or whitening strips containing 9.5% hydrogen peroxide. Treatment was applied for 30 minutes, twice a day, for 1- or 4-weeks. After the last treatment, ten specimens per group were randomly selected to undergo fatigue testing (106 cycles, 3Hz, 20N) while the other ten were subjected to flexural strength testing after ten days of storage in artificial saliva...
2017: PloS One
https://www.readbyqxmd.com/read/28220960/a-comparative-investigation-of-bombyx-mori-silk-fibroin-hydrogels-generated-by-chemical-and-enzymatic-cross-linking
#19
Traian V Chirila, Shuko Suzuki, Chloé Papolla
Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are cross-linked. The subject of this study was the self-cross-linking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported...
February 21, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28193332/analysis-of-agaricus-meleagris-pyranose-dehydrogenase-n-glycosylation-sites-and-performance-of-partially-non-glycosylated-enzymes
#20
Christoph Gonaus, Daniel Maresch, Katharina Schropp, Peter Ó Conghaile, Dónal Leech, Lo Gorton, Clemens K Peterbauer
Pyranose Dehydrogenase 1 from the basidiomycete Agaricus meleagris (AmPDH1) is an oxidoreductase capable of oxidizing a broad variety of sugars. Due to this and its ability of dioxidation of substrates and no side production of hydrogen peroxide, it is studied for use in enzymatic bio-fuel cells. In-vitro deglycosylated AmPDH1 as well as knock-out mutants of the N-glycosylation sites N(75) and N(175), near the active site entrance, were previously shown to improve achievable current densities of graphite electrodes modified with AmPDH1 and an osmium redox polymer acting as a redox mediator, up to 10-fold...
April 2017: Enzyme and Microbial Technology
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