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https://www.readbyqxmd.com/read/28069116/effect-of-hydrogen-peroxide-on-improving-the-heat-stability-of-whey-protein-isolate-solutions
#1
Suresh Sutariya, Hasmukh Patel
Whey protein isolate (WPI) solutions (12.8%w/w protein) were treated with varying concentrations of H2O2 in the range of 0-0.144 H2O2 to protein ratios (HTPR) by the addition of the required quantity of H2O2 and deionized water. The samples were analyzed for heat stability, rheological properties, denaturation level of β-lactoglobulin (β-LG) and α-lactalbumin (α-LA). The samples treated with H2O2 concentration >0.072 (HTPR) showed significant improvement in the heat stability, and decreased whey protein denaturation and aggregation...
May 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28063988/engineered-extracellular-microenvironment-with-a-tunable-mechanical-property-for-controlling-cell-behavior-and-cardiomyogenic-fate-of-cardiac-stem-cells
#2
Min-Young Choi, Jong-Tae Kim, Won-Jin Lee, Yunki Lee, Kyung Min Park, Young-Il Yang, Ki Dong Park
: Endogenous cardiac stem cells (CSCs) are known to play a certain role in the myocardial homeostasis of the adult heart. The extracellular matrix (ECM) surrounding CSCs provides mechanical signals to regulate a variety of cell behaviors, yet the impact in the adult heart of these mechanical properties of ECM on CSC renewal and fate decisions is mostly unknown. To elucidate CSC mechanoresponses at the individual cell and myocardial level, we used the sol-to-gel transitional gelatin-poly(ethylene glycol)-tyramine (GPT) hydrogel with a tunable mechanical property to construct a three-dimensional (3D) matrix for culturing native myocardium and CSCs...
January 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28057569/a-novel-peroxidase-purified-from-marsdenia-megalantha-latex-inhibits-phytopathogenic-fungi-mediated-by-cell-membrane-permeabilization
#3
Henrique P Oliveira, Rodolpho G G Silva, Jose T A Oliveira, Daniele O B Sousa, Mirella L Pereira, Pedro F N Souza, Arlete A Soares, Valdirene M Gomes, Ana C O Monteiro-Moreira, Frederico B M B Moreno, Ilka M Vasconcelos
An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0kDa and a pI of 5.2, shares identity with other peroxidases, and follows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature optima for Mm-POX were 5.0-7.0 and 60°C, respectively. The catalytic activity of Mm-POX was decreased in the presence of classic peroxidase inhibitors including azide, dithiothreitol, ethylenediamine tetraacetic acid, and sodium metabisulfite and high concentrations of Na(+), Mn(+), and salicylic acid...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27989945/specific-adjustments-in-grapevine-leaf-proteome-discriminating-resistant-and-susceptible-grapevine-genotypes-to-plasmopara-viticola
#4
Andreia Figueiredo, Joana Martins, Mónica Sebastiana, Ana Guerreiro, Anabela Silva, Ana Rita Matos, Filipa Monteiro, Maria Salomé Pais, Peter Roepstorff, Ana Varela Coelho
: Grapevine downy mildew is an important disease affecting crop production leading to severe yield losses. This study aims to identify the grapevine cultivar-specific adjustments of leaf proteome that allow the discrimination between resistance and susceptibility towards P. viticola (constitutive (0h) and in after inoculation (6, 12 and 24h). Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry. In addition, we analysed ROS production, antioxidant capacity, lipid peroxidation and gene expression...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27988488/identification-and-structure-elucidation-of-forced-degradation-products-of-the-novel-propionic-acid-derivative-loxoprofen-development-of-stability-indicating-chromatographic-methods-validated-as-per-ich-guidelines
#5
Maya S Eissa, Osama I Abd El-Sattar
Loxoprofen sodium (LOX) is a recently developed novel propionic acid derivative. Owing to its instability under both hydrolytic and oxidative conditions, the development of simple, rapid and sensitive methods for its determination in the presence of its possible forced degradation products becomes essential. Two simple chromatographic methods, high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC), were developed associated with ultraviolet (UV) detection. In HPTLC-densitometric method, the separation of LOX from its degradation products was achieved using silica gel F254 plates and toluene:acetone:acetic acid (1...
December 17, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27987842/synthesis-of-tunable-hydrogels-based-on-o-acetyl-galactoglucomannans-from-spruce
#6
Kajsa Markstedt, Wenyang Xu, Jun Liu, Chunlin Xu, Paul Gatenholm
Hydrogels with tunable mechanical properties based on O-acetyl-galactoglucomannans (GGMs) from spruce functionalized with tyramine, a molecule containing crosslinkable phenolic groups, were prepared. Gel formation was induced by enzymatic crosslinking at the addition of horse radish peroxidase and hydrogen peroxide to the modified GGMs. The degree of substitution determined the hydrogels final properties, and was varied by TEMPO oxidation of GGM to a degree of oxidation from 10 to 60%. GGM and its derivatives were characterized by gas chromatography and high pressure size exclusion chromatography to analyze sugar composition and molar mass, respectively...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27982229/effect-of-two-antioxidants-agents-on-microtensile-bond-strength-to-bleached-enamel
#7
Marina Studart Alencar, Juliana Fraga Soares Bombonatti, Rafael Massunari Maenosono, Ana Flávia Soares, Linda Wang, Rafael Francisco Lia Mondelli
This in vitro study evaluated the effect of sodium bicarbonate and sodium ascorbate on the microtensile bond strength of an etch-and-rinse system to bleached bovine enamel. Sixty bovine enamel blocks (4x4 mm) were flattened and randomly allocated into 5 groups: G1 (negative control): without treatment; G2 (positive control): bleached with 35% hydrogen peroxide (HP); G3: bleached and stored for 7 days in artificial saliva before restorative procedures; G4: bleached and treated with 10% sodium bicarbonate solution for 5 min; G5: bleached and treated with 10% sodium ascorbate hydrogel for 15 min...
September 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27979255/preparation-and-characterisation-of-chlorogenic-acid-gelatin-a-type-of-biologically-active-film-for-coating-preservation
#8
Shalu Fu, Chunhua Wu, Tiantian Wu, Haixia Yu, Shuibing Yang, Yaqin Hu
Chlorogenic acid (CGA), a type of plant polyphenol, was conjugated onto gelatin (Gel) to prepare a novel coating material for the preservation of fresh seafood. The optimal reaction molar ratio of CGA to gelatin (4:1) was determined according to the CGA content in the CGA-Gel conjugate. CGA was confirmed to be successfully conjugated onto gelatin by (1)H nuclear magnetic resonance and Fourier transform-infrared spectroscopy. The antioxidant activity of CGA-Gel was proven to be higher than that of the free CGA in 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical scavenging, hydrogen peroxide scavenging, ferric ion reducing power and lipid oxidation assays...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27935196/an-in-vitro-evaluation-of-human-enamel-surfaces-subjected-to-erosive-challenge-after-bleaching
#9
Maria de Fátima Carvalho Vasconcelos, Andréa Fonseca-Gonçalves, Adílis Kalina Alexandria de França, Urubatan Vieira de Medeiros, Lucianne Cople Maia, Celso Silva Queiroz
OBJECTIVES: This study aimed to evaluate whether tooth enamel bleached with hydrogen peroxide (H2 O2 ) is more susceptible to erosion when compared with unbleached tooth enamel; and whether the presence of calcium (Ca) in the bleaching gel influenced this process. MATERIALS AND METHODS: Enamel blocks were prepared from human molars, and submitted to surface microhardness analysis (baseline). Blocks were prepared and randomly divided into four treatment groups (n = 20): G1 and G2-bleached with 7...
December 9, 2016: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/27909924/tailor-made-biocatalysts-based-on-scarcely-studied-acidic-horseradish-peroxidase-for-biodegradation-of-reactive-dyes
#10
Barbara S Janović, Milica Lj Mićić Vićovac, Zoran M Vujčić, Miroslava T Vujčić
Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzyme was used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol...
December 1, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27891467/an-in-vitro-comparative-study-of-shear-bond-strength-of-composite-resin-to-bleached-enamel-using-three-herbal-antioxidants
#11
Pratap Kumar Mukka, Nanda Kumar Komineni, Sambashivarao Pola, E Soujanya, Anand Ramtheerth Karne, Bhasker Nenavath, Sandeep Shiva, Pallavi Vuppunuthula
INTRODUCTION: If adhesive restorations are carried out imme-diately, the Shear Bond Strength (SBS) to bleached enamel is reduced. The use of antioxidants can be highlighted since it seems to be a more effective alternative to reduce or remove the residual oxygen and immediate improvement in SBS. AIM: The purpose of this in-vitro study was an attempt to regain the lost bond strength, for which, the comparison of SBS of composite resin to bleached enamel was carried out using various antioxidants i...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27872240/the-staphylococcus-aureus-airsr-two-component-system-mediates-reactive-oxygen-species-resistance-via-transcriptional-regulation-of-staphyloxanthin-production
#12
Jeffrey W Hall, Junshu Yang, Haiyong Guo, Yinduo Ji
Staphylococcus aureus is an important opportunistic pathogen and etiologically agent of many hospital- and community-acquired infections. The golden pigment, staphyloxanthin, of S. aureus colonies distinguishes it from other staphylococci and related Gram-positive cocci. Staphyloxanthin is the product of a series of biosynthetic steps that produce a unique membrane-embedded C (30) golden carotenoid and is an important antioxidant. We observed a strain with an inducible airR overexpression cassette had noticeably increased staphyloxanthin production compared to the wild-type strain under aerobic culturing conditions...
November 21, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27818650/functional-characterization-of-pkni-rv2159c-interaction-in-redox-homeostasis-of-mycobacterium-tuberculosis
#13
Arunkumar Venkatesan, Kannan Palaniyandi, Divakar Sharma, Deepa Bisht, Sujatha Narayanan
Mycobacterium tuberculosis adapts to stress conditions by responding to the signals from its external environment. M. tuberculosis genome encodes 11 eukaryotic like serine/threonine protein kinases (STPK) and their importance in regulating the physiology and virulence of the bacteria are being explored. Previous study from our lab identified the M. tuberculosis STPK, PknI interacts with two peroxidase proteins such as Rv2159c and Rv0148. In this study, we have characterized the biological function behind the PknI-Rv2159c interaction in M...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27790286/sol-gel-encapsulation-of-au-nanoparticles-in-hybrid-silica-improves-gold-oxidation-catalysis
#14
Rosaria Ciriminna, Valerica Pandarus, Riccardo Delisi, Antonino Scurria, Maria Pia Casaletto, Francesco Giordano, François Béland, Mario Pagliaro
BACKGROUND: The introduction of an heterogeneously catalyzed gold-based alcohol oxidation process of broad applicability using a clean primary oxidant would be highly desirable. Gold is non toxic and carbonyl and carboxyl compounds are widely used to produce medicines, plastics, colorants, paints, detergents, fragrances, flavors, and other valued functional products. RESULTS: The sol-gel entrapment of gold nanoparticles in hybrid silica improves gold-based oxidation catalysis applied to the selective oxidation of alcohols with aqueous hydrogen peroxide as eco-friendly primary oxidant...
2016: Chemistry Central Journal
https://www.readbyqxmd.com/read/27788422/protein-glutathionylation-protects-wheat-triticum-aestivum-var-sonalika-against-fusarium-induced-oxidative-stress
#15
Subhalaxmi Mohapatra, Bhabatosh Mittra
Fusarium induced oxidative stress could be recovered by reversible protein oxidative modification through the process of glutathionylation in co-stressed (low-dose (50 μM) Cd(2+) pre-treatment followed by Fusarium inoculation) wheat seedlings. Co-stressed seedlings showed low disease severity index as compared to Fusarium infected seedlings. A reduced level of hydrogen peroxide (H2O2) and carbonyl contents due to irreversible protein oxidation were observed in co-stressed seedlings as compared to Fusarium infected seedlings...
December 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27770885/horseradish-peroxidase-and-toluidine-blue-covalently-immobilized-leak-free-sol-gel-composite-biosensor-for-hydrogen-peroxide
#16
K Thenmozhi, S Sriman Narayanan
The enzyme horseradish peroxidase and the water-soluble mediator toluidine blue were covalently immobilized to 3-aminopropyl trimethoxy silane precursor through glutaraldehyde crosslinker. A rigid ceramic composite electrode was fabricated from this modified silane along with graphite powder, which resulted in an amperometric biosensor for H2O2. The electrochemical behaviour of the modified biosensor was monitored using cyclic voltammetry in the potential range of 0.2V to -0.4V vs SCE. The biosensor exhibited a stable voltammogram with cathodic peak at -0...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27713832/a-comparative-study-of-enzyme-initiators-for-crosslinking-phenol-functionalized-hydrogels-for-cell-encapsulation
#17
Justine J Roberts, Pratibha Naudiyal, Khoon S Lim, Laura A Poole-Warren, Penny J Martens
BACKGROUND: Dityrosine crosslinking in proteins is a bioinspired method of forming hydrogels. This study compares oxidative enzyme initiators for their relative crosslinking efficiency and cytocompatibility using the same phenol group and the same material platform. Four common enzyme and enzyme-like oxidative initiators were probed for resulting material properties and cell viability post-encapsulation. RESULTS: All four initiators can be used to form phenol-crosslinked hydrogels, however gelation rates are dependent on enzyme type, concentration, and the oxidant...
2016: Biomaterials Research
https://www.readbyqxmd.com/read/27708725/influence-of-enamel-thickness-on-bleaching-efficacy-an-in-depth-color-analysis
#18
Juliana do Carmo Públio, Maria Beatriz Freitas D'Arce, Anderson Catelan, Gláucia Maria Bovi Ambrosano, Flávio Henrique Baggio Aguiar, José Roberto Lovadino, Débora Alves Nunes Leite Lima
This study evaluated the influence of different enamel thicknesses and bleaching agents on treatment efficacy in-depth by spectrophotometry color analysis. Eighty bovine dental fragments were previously stained in black tea solution and randomly assigned into eight groups (n=10), 1.75mm dentin thickness and different enamel thicknesses as follows: 0.5mm, 1.0mm planned, 1.0mm unplanned (aprismatic enamel), and absence of enamel. The 10% carbamide peroxide (CP) and 35% hydrogen peroxide (HP) bleaching gels were applied on the enamel surface following the manufacturer's recommendations...
2016: Open Dentistry Journal
https://www.readbyqxmd.com/read/27701501/dental-bleaching-with-ozone-effects-on-color-and-enamel-microhardness
#19
Manuella Sca Santana, Enrico C Bridi, Ricardo S Navarro, Carlos J de Lima, Adriana B Fernandes, Flávia Lb do Amaral, Fabiana Mg França, Cecilia P Turssi, Roberta T Basting
The aim of this in vitro study was to evaluate the effects of dental bleaching with ozone (O3) on color change and enamel microhardness. Enamel blocks (3 x 3 x 3mm) were randomly distributed for treatments (n=10). Color change (ΔE) and Knoop microhardness of the enamel blocks were evaluated before and after the following treatments: C - deionized water (control); HP - 37.5% hydrogen peroxide (Pola Office+/ SDI); PLA - placebo gel; O3 - ozone; and O2 - oxygen. Four 8-minute applications were used for HP and PLA, and one 19-minute application for O3 and O2...
April 2016: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/27649456/clinical-evaluation-of-genotoxicity-of-in-office-bleaching
#20
M Rezende, J L De Geus, A D Loguercio, A Reis, D Kossatz
OBJECTIVE: The aim of this study was to evaluate the genotoxicity of in-office bleaching with 35% hydrogen peroxide in epithelial cells from the gingival and lip tissues. METHODS AND MATERIALS: Thirty volunteers with central incisors shade A1 or darker were selected for this study. The gingival tissue of the teeth to be bleached was isolated with a light-polymerized resin dam, and the 35% hydrogen peroxide gel was administered during three 15-minute applications over the course of the 45-minute application period...
November 2016: Operative Dentistry
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