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Hydrogen peroxide gel

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https://www.readbyqxmd.com/read/28336378/optimized-biodegradable-polymeric-reservoir-mediated-local-and-sustained-co-delivery-of-dendritic-cells-and-oncolytic-adenovirus-co-expressing-il-12-and-gm-csf-for-cancer-immunotherapy
#1
Eonju Oh, Jung-Eun Oh, JinWoo Hong, YoonHo Chung, Yunki Lee, Ki Dong Park, Sungwan Kim, Chae-Ok Yun
Administration of dendritic cells (DCs) combined with oncolytic adenovirus (Ad) expressing antitumor cytokines induces a potent antitumor effect and antitumor immunity by ameliorating the immunosuppressive tumor microenvironment. However, this combination therapy has significant limitations due to rapid dissemination and inactivation of the therapeutics at the tumor site, necessitating multiple injections of both therapeutics. To overcome these limitations, we have utilized gelatin-based hydrogel to co-deliver oncolytic Ad co-expressing interleukin (IL)-12 and granulocyte-macrophage colony-stimulating factor (GM-CSF) (oAd) and DCs for sustained release of both therapeutics...
March 20, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28330261/purification-and-characterization-of-laccase-from-marasmius-species-bbkav79-and-effective-decolorization-of-selected-textile-dyes
#2
A B Vantamuri, B B Kaliwal
A novel laccase-producing white-rot fungus, Marasmius sp. BBKAV79 (Genbank Accession Number-KP455496, KP455497), was isolated and subjected to purification, characterization and dye decolorization study. The purified enzyme was obtained with a specific activity of 0.226 U mg(-1) protein and a final yield of 13.5 %. The enzyme was found to be a monomeric protein with a molecular mass of ~75 kDa as estimated by non-denaturing polyacrylamide gel electrophoresis (PAGE) and further confirmed with zymogram analysis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28303471/effect-of-acidity-of-in-office-bleaching-gels-on-tooth-sensitivity-and-whitening-a-two-center-double-blind-randomized-clinical-trial
#3
A D Loguercio, F Servat, R Stanislawczuk, A Mena-Serrano, M Rezende, M V Prieto, V Cereño, M F Rojas, K Ortega, E Fernandez, A Reis
OBJECTIVES: The study aimed to compare the tooth sensitivity (TS) and bleaching efficacy of two hydrogen peroxide gels with different pHs (acid pH [Pola Office, SDI] and the neutral pH [Pola Office+, SDI]) used for in-office bleaching. MATERIALS AND METHODS: Fifty-four patients from Brazil and Chile, with right superior incisor darker than A2, were selected for this double-blind, split-mouth randomized trial. Teeth were bleached in two sessions, with 1-week interval...
March 17, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28285876/one-pot-synthesis-of-a-new-2-substituted-1-2-3-triazole-1-oxide-derivative-from-dipyridyl-ketone-and-isonitrosoacetophenone-hydrazone-nickel-ii-complex-dna-binding-and-cleavage-properties
#4
Ramazan Gup, Oktay Erer, Nefise Dilek
An efficient and simple one-pot synthesis of a new 1,2,3-triazole-1-oxide via reaction between isonitrosoacetophenone hydrazone and dipyridyl ketone in the EtOH/AcOH at room temperature has been developed smoothly in high yield. The reaction proceeds via metal salt free, in-situ formation of asymmetric azine followed by cyclization to provide 1,2,3-triazole 1-oxide compound. It has been structurally characterized. The 1:1 ratio reaction of the 1,2,3-triazole 1-oxide ligand with nickel(II) chloride gives the mononuclear complex [Ni(L)(DMF)Cl2], hexa-coordinated within an octahedral geometry...
March 3, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28278191/effect-of-tooth-whitening-strips-on-fatigue-resistance-and-flexural-strength-of-bovine-dentin-in-vitro
#5
Laura E Tam, Namhee Kim, Grace M De Souza
OBJECTIVE: To determine the effects of whitening strips on bovine dentin fatigue resistance and flexural strength in vitro. MATERIALS AND METHODS: A total of eighty bovine dentin specimens (2x2x17mm) were treated with either: control glycerine gel on plastic film wrap or whitening strips containing 9.5% hydrogen peroxide. Treatment was applied for 30 minutes, twice a day, for 1- or 4-weeks. After the last treatment, ten specimens per group were randomly selected to undergo fatigue testing (106 cycles, 3Hz, 20N) while the other ten were subjected to flexural strength testing after ten days of storage in artificial saliva...
2017: PloS One
https://www.readbyqxmd.com/read/28220960/a-comparative-investigation-of-bombyx-mori-silk-fibroin-hydrogels-generated-by-chemical-and-enzymatic-crosslinking
#6
Traian V Chirila, Shuko Suzuki, Chloé Papolla
Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are crosslinked. The subject of this study was the self-crosslinking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported...
February 21, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28193332/analysis-of-agaricus-meleagris-pyranose-dehydrogenase-n-glycosylation-sites-and-performance-of-partially-non-glycosylated-enzymes
#7
Christoph Gonaus, Daniel Maresch, Katharina Schropp, Peter Ó Conghaile, Dónal Leech, Lo Gorton, Clemens K Peterbauer
Pyranose Dehydrogenase 1 from the basidiomycete Agaricus meleagris (AmPDH1) is an oxidoreductase capable of oxidizing a broad variety of sugars. Due to this and its ability of dioxidation of substrates and no side production of hydrogen peroxide, it is studied for use in enzymatic bio-fuel cells. In-vitro deglycosylated AmPDH1 as well as knock-out mutants of the N-glycosylation sites N(75) and N(175), near the active site entrance, were previously shown to improve achievable current densities of graphite electrodes modified with AmPDH1 and an osmium redox polymer acting as a redox mediator, up to 10-fold...
April 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28189114/nano-scale-synthesis-of-the-complex-silicate-minerals-forsterite-and-enstatite
#8
Lawrence M Anovitz, Adam J Rondinone, Lindsay Sochalski-Kolbus, Jörgen Rosenqvist, Michael C Cheshire
Olivine is a relatively common family of silicate minerals in many terrestrial and extraterrestrial environments, and is also useful as a refractory ceramic. A capability to synthesize fine particles of olivine will enable additional studies on surface reactivity under geologically relevant conditions. This paper presents a method for the synthesis of nanocrystalline samples of the magnesium end-member, forsterite (Mg2SiO4) in relatively large batches (15-20g) using a sol-gel/surfactant approach. Magnesium methoxide and tetraethylorthosilicate (TEOS) are refluxed in a toluene/methanol mixture using dodecylamine as a surfactant and tert-butyl amine and water as hydrolysis agents...
June 1, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28185045/identification-and-in-silico-analysis-of-major-redox-modulated-proteins-from-brassica-juncea-seedlings-using-2d-redox-sds-page-2-dimensional-diagonal-redox-sodium-dodecyl-sulfate-polyacrylamide-gel-electrophoresis
#9
Satya Prakash Chaurasia, Renu Deswal
The thiol-disulphide exchange regulates the activity of proteins by redox modulation. Many studies to analyze reactive oxygen species (ROS), particularly, hydrogen peroxide (H2O2) induced changes in the gene expression have been reported, but efforts to detect H2O2 modified proteins are comparatively few. Two-dimensional diagonal redox sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) was used to detect polypeptides which undergo thiol-disulphide exchange in Brassica juncea seedlings following H2O2 (10 mM) treatment for 30 min...
February 2017: Protein Journal
https://www.readbyqxmd.com/read/28182856/silica-sol-gel-optical-biosensors-ultrahigh-enzyme-loading-capacity-on-thin-films-via-kinetic-doping
#10
Matthew S Crosley, Wai Tak Yip
Easy to use and easy to produce biosensors would have a huge range of applications. To reach this goal many see the incorporation of a protein into a sol-gel network as one of the most viable options. The current most prevalent technique of predoping presents inherent limits on the concentration possible for the resulting thin film. In this study we demonstrate a new process utilizing the newly developed kinetic doping method to load silica sol-gel thin films with cytochrome C (CytC) and horseradish peroxidase (HRP)...
February 22, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28155851/robust-tissue-growth-and-angiogenesis-in-large-sized-scaffold-by-reducing-h2o2-mediated-oxidative-stress
#11
Girdhari Rijal, Byoung Soo Kim, Falguni Pati, Dong-Heon Ha, Sung Won Kim, Dong-Woo Cho
The implantation of cell-seeded large-sized scaffold often results in insufficient tissue regeneration, which is still a challenge for successful grafting. Excess hydrogen peroxide (H2O2) released by cells propagates oxidative stress, which is the primary cause of tissue injury leading to failure in tissue regeneration. Hence, preventing tissue from oxidative damage becomes imperative. For the first time, we entrapped catalase, an antioxidant in a scaffold as a novel approach in bioengineering to prevent tissue from H2O2-induced damage...
February 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28149472/effect-of-tooth-bleaching-on-the-carbonate-concentration-in-dental-enamel-by-raman-spectroscopy
#12
Tatiana Vargas-Koudriavtsev, Óscar-Andrey Herrera-Sancho
BACKGROUND: There are not many studies evaluating the effects of surface treatments at the molecular level. The aim of this in vitro study was to analyze the concentration of carbonate molecules in dental enamel by Raman spectroscopy after the application of in-office and home whitening agents. MATERIAL AND METHODS: Sixty human teeth were randomly divided into six groups and exposed to three different home bleaching gels (Day White) and three in-office whitening agents (Zoom! Whitespeed and PolaOffice) according to the manufacturer´s instructions...
January 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28091877/influence-of-enamel-dentin-thickness-on-the-toxic-and-esthetic-effects-of-experimental-in-office-bleaching-protocols
#13
C C de Oliveira Duque, D G Soares, F G Basso, J Hebling, C A de Souza Costa
OBJECTIVES: This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs). MATERIALS AND METHODS: A 10% hydrogen peroxide (H2O2) gel was applied for 3 × 15, 1 × 15, or 1 × 5 min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1 h)...
January 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28069116/effect-of-hydrogen-peroxide-on-improving-the-heat-stability-of-whey-protein-isolate-solutions
#14
Suresh Sutariya, Hasmukh Patel
Whey protein isolate (WPI) solutions (12.8%w/w protein) were treated with varying concentrations of H2O2 in the range of 0-0.144 H2O2 to protein ratios (HTPR) by the addition of the required quantity of H2O2 and deionized water. The samples were analyzed for heat stability, rheological properties, denaturation level of β-lactoglobulin (β-LG) and α-lactalbumin (α-LA). The samples treated with H2O2 concentration >0.072 (HTPR) showed significant improvement in the heat stability, and decreased whey protein denaturation and aggregation...
May 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28063988/engineered-extracellular-microenvironment-with-a-tunable-mechanical-property-for-controlling-cell-behavior-and-cardiomyogenic-fate-of-cardiac-stem-cells
#15
Min-Young Choi, Jong-Tae Kim, Won-Jin Lee, Yunki Lee, Kyung Min Park, Young-Il Yang, Ki Dong Park
Endogenous cardiac stem cells (CSCs) are known to play a certain role in the myocardial homeostasis of the adult heart. The extracellular matrix (ECM) surrounding CSCs provides mechanical signals to regulate a variety of cell behaviors, yet the impact in the adult heart of these mechanical properties of ECM on CSC renewal and fate decisions is mostly unknown. To elucidate CSC mechanoresponses at the individual cell and myocardial level, we used the sol-to-gel transitional gelatin-poly(ethylene glycol)-tyramine (GPT) hydrogel with a tunable mechanical property to construct a three-dimensional (3D) matrix for culturing native myocardium and CSCs...
January 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28057569/a-novel-peroxidase-purified-from-marsdenia-megalantha-latex-inhibits-phytopathogenic-fungi-mediated-by-cell-membrane-permeabilization
#16
Henrique P Oliveira, Rodolpho G G Silva, Jose T A Oliveira, Daniele O B Sousa, Mirella L Pereira, Pedro F N Souza, Arlete A Soares, Valdirene M Gomes, Ana C O Monteiro-Moreira, Frederico B M B Moreno, Ilka M Vasconcelos
An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0kDa and a pI of 5.2, shares identity with other peroxidases, and follows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature optima for Mm-POX were 5.0-7.0 and 60°C, respectively. The catalytic activity of Mm-POX was decreased in the presence of classic peroxidase inhibitors including azide, dithiothreitol, ethylenediamine tetraacetic acid, and sodium metabisulfite and high concentrations of Na(+), Mn(+), and salicylic acid...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27989945/specific-adjustments-in-grapevine-leaf-proteome-discriminating-resistant-and-susceptible-grapevine-genotypes-to-plasmopara-viticola
#17
Andreia Figueiredo, Joana Martins, Mónica Sebastiana, Ana Guerreiro, Anabela Silva, Ana Rita Matos, Filipa Monteiro, Maria Salomé Pais, Peter Roepstorff, Ana Varela Coelho
Grapevine downy mildew is an important disease affecting crop production leading to severe yield losses. This study aims to identify the grapevine cultivar-specific adjustments of leaf proteome that allow the discrimination between resistance and susceptibility towards P. viticola (constitutive (0h) and in after inoculation (6, 12 and 24h). Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry. In addition, we analysed ROS production, antioxidant capacity, lipid peroxidation and gene expression...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27988488/identification-and-structure-elucidation-of-forced-degradation-products-of-the-novel-propionic-acid-derivative-loxoprofen-development-of-stability-indicating-chromatographic-methods-validated-as-per-ich-guidelines
#18
Maya S Eissa, Osama I Abd El-Sattar
Loxoprofen sodium (LOX) is a recently developed novel propionic acid derivative. Owing to its instability under both hydrolytic and oxidative conditions, the development of simple, rapid and sensitive methods for its determination in the presence of its possible forced degradation products becomes essential. Two simple chromatographic methods, high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC), were developed associated with ultraviolet (UV) detection. In HPTLC-densitometric method, the separation of LOX from its degradation products was achieved using silica gel F254 plates and toluene:acetone:acetic acid (1...
December 17, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27987842/synthesis-of-tunable-hydrogels-based-on-o-acetyl-galactoglucomannans-from-spruce
#19
Kajsa Markstedt, Wenyang Xu, Jun Liu, Chunlin Xu, Paul Gatenholm
Hydrogels with tunable mechanical properties based on O-acetyl-galactoglucomannans (GGMs) from spruce functionalized with tyramine, a molecule containing crosslinkable phenolic groups, were prepared. Gel formation was induced by enzymatic crosslinking at the addition of horse radish peroxidase and hydrogen peroxide to the modified GGMs. The degree of substitution determined the hydrogels final properties, and was varied by TEMPO oxidation of GGM to a degree of oxidation from 10 to 60%. GGM and its derivatives were characterized by gas chromatography and high pressure size exclusion chromatography to analyze sugar composition and molar mass, respectively...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27982229/effect-of-two-antioxidants-agents-on-microtensile-bond-strength-to-bleached-enamel
#20
Marina Studart Alencar, Juliana Fraga Soares Bombonatti, Rafael Massunari Maenosono, Ana Flávia Soares, Linda Wang, Rafael Francisco Lia Mondelli
This in vitro study evaluated the effect of sodium bicarbonate and sodium ascorbate on the microtensile bond strength of an etch-and-rinse system to bleached bovine enamel. Sixty bovine enamel blocks (4x4 mm) were flattened and randomly allocated into 5 groups: G1 (negative control): without treatment; G2 (positive control): bleached with 35% hydrogen peroxide (HP); G3: bleached and stored for 7 days in artificial saliva before restorative procedures; G4: bleached and treated with 10% sodium bicarbonate solution for 5 min; G5: bleached and treated with 10% sodium ascorbate hydrogel for 15 min...
September 2016: Brazilian Dental Journal
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