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https://www.readbyqxmd.com/read/28220960/a-comparative-investigation-of-bombyx-mori-silk-fibroin-hydrogels-generated-by-chemical-and-enzymatic-crosslinking
#1
Traian V Chirila, Shuko Suzuki, Chloé Papolla
Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are crosslinked. The subject of this study was the self-crosslinking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported...
February 21, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28193332/analysis-of-agaricus-meleagris-pyranose-dehydrogenase-n-glycosylation-sites-and-performance-of-partially-non-glycosylated-enzymes
#2
Christoph Gonaus, Daniel Maresch, Katharina Schropp, Peter Ó Conghaile, Dónal Leech, Lo Gorton, Clemens K Peterbauer
Pyranose Dehydrogenase 1 from the basidiomycete Agaricus meleagris (AmPDH1) is an oxidoreductase capable of oxidizing a broad variety of sugars. Due to this and its ability of dioxidation of substrates and no side production of hydrogen peroxide, it is studied for use in enzymatic bio-fuel cells. In-vitro deglycosylated AmPDH1 as well as knock-out mutants of the N-glycosylation sites N(75) and N(175), near the active site entrance, were previously shown to improve achievable current densities of graphite electrodes modified with AmPDH1 and an osmium redox polymer acting as a redox mediator, up to 10-fold...
April 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28189114/nano-scale-synthesis-of-the-complex-silicate-minerals-forsterite-and-enstatite
#3
Lawrence M Anovitz, Adam J Rondinone, Lindsay Sochalski-Kolbus, Jörgen Rosenqvist, Michael C Cheshire
Olivine is a relatively common family of silicate minerals in many terrestrial and extraterrestrial environments, and is also useful as a refractory ceramic. A capability to synthesize fine particles of olivine will enable additional studies on surface reactivity under geologically relevant conditions. This paper presents a method for the synthesis of nanocrystalline samples of the magnesium end-member, forsterite (Mg2SiO4) in relatively large batches (15-20g) using a sol-gel/surfactant approach. Magnesium methoxide and tetraethylorthosilicate (TEOS) are refluxed in a toluene/methanol mixture using dodecylamine as a surfactant and tert-butyl amine and water as hydrolysis agents...
January 18, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28185045/identification-and-in-silico-analysis-of-major-redox-modulated-proteins-from-brassica-juncea-seedlings-using-2d-redox-sds-page-2-dimensional-diagonal-redox-sodium-dodecyl-sulfate-polyacrylamide-gel-electrophoresis
#4
Satya Prakash Chaurasia, Renu Deswal
The thiol-disulphide exchange regulates the activity of proteins by redox modulation. Many studies to analyze reactive oxygen species (ROS), particularly, hydrogen peroxide (H2O2) induced changes in the gene expression have been reported, but efforts to detect H2O2 modified proteins are comparatively few. Two-dimensional diagonal redox sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) was used to detect polypeptides which undergo thiol-disulphide exchange in Brassica juncea seedlings following H2O2 (10 mM) treatment for 30 min...
February 9, 2017: Protein Journal
https://www.readbyqxmd.com/read/28182856/silica-sol-gel-optical-biosensors-ultrahigh-enzyme-loading-capacity-on-thin-films-via-kinetic-doping
#5
Matthew Scott Crosley, Wai Tak Yip
Easy to use and easy to produce biosensors would have a huge range of applications. To reach this goal many see the incorporation of a protein into a sol-gel network as one of the most viable options. The current most prevalent technique of pre-doping presents inherent limits on the concentration possible for the resulting thin film. In this study we demonstrate a new process utilizing the newly developed kinetic doping method to load silica sol-gel thin films with Cytochrome C (CytC) and Horseradish Peroxidase (HRP)...
February 9, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28155851/robust-tissue-growth-and-angiogenesis-in-large-sized-scaffold-by-reducing-h2o2-mediated-oxidative-stress
#6
Girdhari Rijal, Byoung Soo Kim, Falguni Pati, Dong-Heon Ha, Sung Won Kim, Dong-Woo Cho
The implantation of cell-seeded large-sized scaffold often results in insufficient tissue regeneration, which is still a challenge for successful grafting. Excess hydrogen peroxide (H2O2) released by cells propagates oxidative stress, which is the primary cause of tissue injury leading to failure in tissue regeneration. Hence, preventing tissue from oxidative damage becomes imperative. For the first time, we entrapped catalase, an antioxidant in a scaffold as a novel approach in bioengineering to prevent tissue from H2O2-induced damage...
February 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28149472/effect-of-tooth-bleaching-on-the-carbonate-concentration-in-dental-enamel-by-raman-spectroscopy
#7
Tatiana Vargas-Koudriavtsev, Óscar-Andrey Herrera-Sancho
BACKGROUND: There are not many studies evaluating the effects of surface treatments at the molecular level. The aim of this in vitro study was to analyze the concentration of carbonate molecules in dental enamel by Raman spectroscopy after the application of in-office and home whitening agents. MATERIAL AND METHODS: Sixty human teeth were randomly divided into six groups and exposed to three different home bleaching gels (Day White) and three in-office whitening agents (Zoom! Whitespeed and PolaOffice) according to the manufacturer´s instructions...
January 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28091877/influence-of-enamel-dentin-thickness-on-the-toxic-and-esthetic-effects-of-experimental-in-office-bleaching-protocols
#8
C C de Oliveira Duque, D G Soares, F G Basso, J Hebling, C A de Souza Costa
OBJECTIVES: This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs). MATERIALS AND METHODS: A 10% hydrogen peroxide (H2O2) gel was applied for 3 × 15, 1 × 15, or 1 × 5 min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1 h)...
January 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28069116/effect-of-hydrogen-peroxide-on-improving-the-heat-stability-of-whey-protein-isolate-solutions
#9
Suresh Sutariya, Hasmukh Patel
Whey protein isolate (WPI) solutions (12.8%w/w protein) were treated with varying concentrations of H2O2 in the range of 0-0.144 H2O2 to protein ratios (HTPR) by the addition of the required quantity of H2O2 and deionized water. The samples were analyzed for heat stability, rheological properties, denaturation level of β-lactoglobulin (β-LG) and α-lactalbumin (α-LA). The samples treated with H2O2 concentration >0.072 (HTPR) showed significant improvement in the heat stability, and decreased whey protein denaturation and aggregation...
May 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28063988/engineered-extracellular-microenvironment-with-a-tunable-mechanical-property-for-controlling-cell-behavior-and-cardiomyogenic-fate-of-cardiac-stem-cells
#10
Min-Young Choi, Jong-Tae Kim, Won-Jin Lee, Yunki Lee, Kyung Min Park, Young-Il Yang, Ki Dong Park
: Endogenous cardiac stem cells (CSCs) are known to play a certain role in the myocardial homeostasis of the adult heart. The extracellular matrix (ECM) surrounding CSCs provides mechanical signals to regulate a variety of cell behaviors, yet the impact in the adult heart of these mechanical properties of ECM on CSC renewal and fate decisions is mostly unknown. To elucidate CSC mechanoresponses at the individual cell and myocardial level, we used the sol-to-gel transitional gelatin-poly(ethylene glycol)-tyramine (GPT) hydrogel with a tunable mechanical property to construct a three-dimensional (3D) matrix for culturing native myocardium and CSCs...
January 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28057569/a-novel-peroxidase-purified-from-marsdenia-megalantha-latex-inhibits-phytopathogenic-fungi-mediated-by-cell-membrane-permeabilization
#11
Henrique P Oliveira, Rodolpho G G Silva, Jose T A Oliveira, Daniele O B Sousa, Mirella L Pereira, Pedro F N Souza, Arlete A Soares, Valdirene M Gomes, Ana C O Monteiro-Moreira, Frederico B M B Moreno, Ilka M Vasconcelos
An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0kDa and a pI of 5.2, shares identity with other peroxidases, and follows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature optima for Mm-POX were 5.0-7.0 and 60°C, respectively. The catalytic activity of Mm-POX was decreased in the presence of classic peroxidase inhibitors including azide, dithiothreitol, ethylenediamine tetraacetic acid, and sodium metabisulfite and high concentrations of Na(+), Mn(+), and salicylic acid...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27989945/specific-adjustments-in-grapevine-leaf-proteome-discriminating-resistant-and-susceptible-grapevine-genotypes-to-plasmopara-viticola
#12
Andreia Figueiredo, Joana Martins, Mónica Sebastiana, Ana Guerreiro, Anabela Silva, Ana Rita Matos, Filipa Monteiro, Maria Salomé Pais, Peter Roepstorff, Ana Varela Coelho
: Grapevine downy mildew is an important disease affecting crop production leading to severe yield losses. This study aims to identify the grapevine cultivar-specific adjustments of leaf proteome that allow the discrimination between resistance and susceptibility towards P. viticola (constitutive (0h) and in after inoculation (6, 12 and 24h). Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry. In addition, we analysed ROS production, antioxidant capacity, lipid peroxidation and gene expression...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27988488/identification-and-structure-elucidation-of-forced-degradation-products-of-the-novel-propionic-acid-derivative-loxoprofen-development-of-stability-indicating-chromatographic-methods-validated-as-per-ich-guidelines
#13
Maya S Eissa, Osama I Abd El-Sattar
Loxoprofen sodium (LOX) is a recently developed novel propionic acid derivative. Owing to its instability under both hydrolytic and oxidative conditions, the development of simple, rapid and sensitive methods for its determination in the presence of its possible forced degradation products becomes essential. Two simple chromatographic methods, high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC), were developed associated with ultraviolet (UV) detection. In HPTLC-densitometric method, the separation of LOX from its degradation products was achieved using silica gel F254 plates and toluene:acetone:acetic acid (1...
December 17, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27987842/synthesis-of-tunable-hydrogels-based-on-o-acetyl-galactoglucomannans-from-spruce
#14
Kajsa Markstedt, Wenyang Xu, Jun Liu, Chunlin Xu, Paul Gatenholm
Hydrogels with tunable mechanical properties based on O-acetyl-galactoglucomannans (GGMs) from spruce functionalized with tyramine, a molecule containing crosslinkable phenolic groups, were prepared. Gel formation was induced by enzymatic crosslinking at the addition of horse radish peroxidase and hydrogen peroxide to the modified GGMs. The degree of substitution determined the hydrogels final properties, and was varied by TEMPO oxidation of GGM to a degree of oxidation from 10 to 60%. GGM and its derivatives were characterized by gas chromatography and high pressure size exclusion chromatography to analyze sugar composition and molar mass, respectively...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27982229/effect-of-two-antioxidants-agents-on-microtensile-bond-strength-to-bleached-enamel
#15
Marina Studart Alencar, Juliana Fraga Soares Bombonatti, Rafael Massunari Maenosono, Ana Flávia Soares, Linda Wang, Rafael Francisco Lia Mondelli
This in vitro study evaluated the effect of sodium bicarbonate and sodium ascorbate on the microtensile bond strength of an etch-and-rinse system to bleached bovine enamel. Sixty bovine enamel blocks (4x4 mm) were flattened and randomly allocated into 5 groups: G1 (negative control): without treatment; G2 (positive control): bleached with 35% hydrogen peroxide (HP); G3: bleached and stored for 7 days in artificial saliva before restorative procedures; G4: bleached and treated with 10% sodium bicarbonate solution for 5 min; G5: bleached and treated with 10% sodium ascorbate hydrogel for 15 min...
September 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27979255/preparation-and-characterisation-of-chlorogenic-acid-gelatin-a-type-of-biologically-active-film-for-coating-preservation
#16
Shalu Fu, Chunhua Wu, Tiantian Wu, Haixia Yu, Shuibing Yang, Yaqin Hu
Chlorogenic acid (CGA), a type of plant polyphenol, was conjugated onto gelatin (Gel) to prepare a novel coating material for the preservation of fresh seafood. The optimal reaction molar ratio of CGA to gelatin (4:1) was determined according to the CGA content in the CGA-Gel conjugate. CGA was confirmed to be successfully conjugated onto gelatin by (1)H nuclear magnetic resonance and Fourier transform-infrared spectroscopy. The antioxidant activity of CGA-Gel was proven to be higher than that of the free CGA in 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical scavenging, hydrogen peroxide scavenging, ferric ion reducing power and lipid oxidation assays...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27935196/an-in-vitro-evaluation-of-human-enamel-surfaces-subjected-to-erosive-challenge-after-bleaching
#17
Maria de Fátima Carvalho Vasconcelos, Andréa Fonseca-Gonçalves, Adílis Kalina Alexandria de França, Urubatan Vieira de Medeiros, Lucianne Cople Maia, Celso Silva Queiroz
OBJECTIVES: This study aimed to evaluate whether tooth enamel bleached with hydrogen peroxide (H2 O2 ) is more susceptible to erosion when compared with unbleached tooth enamel; and whether the presence of calcium (Ca) in the bleaching gel influenced this process. MATERIALS AND METHODS: Enamel blocks were prepared from human molars, and submitted to surface microhardness analysis (baseline). Blocks were prepared and randomly divided into four treatment groups (n = 20): G1 and G2-bleached with 7...
December 9, 2016: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/27909924/tailor-made-biocatalysts-based-on-scarcely-studied-acidic-horseradish-peroxidase-for-biodegradation-of-reactive-dyes
#18
Barbara S Janović, Milica Lj Mićić Vićovac, Zoran M Vujčić, Miroslava T Vujčić
Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzyme was used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol...
December 1, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27891467/an-in-vitro-comparative-study-of-shear-bond-strength-of-composite-resin-to-bleached-enamel-using-three-herbal-antioxidants
#19
Pratap Kumar Mukka, Nanda Kumar Komineni, Sambashivarao Pola, E Soujanya, Anand Ramtheerth Karne, Bhasker Nenavath, Sandeep Shiva, Pallavi Vuppunuthula
INTRODUCTION: If adhesive restorations are carried out imme-diately, the Shear Bond Strength (SBS) to bleached enamel is reduced. The use of antioxidants can be highlighted since it seems to be a more effective alternative to reduce or remove the residual oxygen and immediate improvement in SBS. AIM: The purpose of this in-vitro study was an attempt to regain the lost bond strength, for which, the comparison of SBS of composite resin to bleached enamel was carried out using various antioxidants i...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27872240/the-staphylococcus-aureus-airsr-two-component-system-mediates-reactive-oxygen-species-resistance-via-transcriptional-regulation-of-staphyloxanthin-production
#20
Jeffrey W Hall, Junshu Yang, Haiyong Guo, Yinduo Ji
Staphylococcus aureus is an important opportunistic pathogen and is the etiological agent of many hospital- and community-acquired infections. The golden pigment, staphyloxanthin, of S. aureus colonies distinguishes it from other staphylococci and related Gram-positive cocci. Staphyloxanthin is the product of a series of biosynthetic steps that produce a unique membrane-embedded C30 golden carotenoid and is an important antioxidant. We observed that a strain with an inducible airR overexpression cassette had noticeably increased staphyloxanthin production compared to the wild-type strain under aerobic culturing conditions...
February 2017: Infection and Immunity
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