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Heat activated bleaching

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https://www.readbyqxmd.com/read/28802271/antibiofilm-effect-of-poly-vinyl-alcohol-co-ethylene-pva-co-pe-halamine-film-against-listeria-innocua-and-escherichia-coli-o157-h7
#1
Andrea Cossu, Yang Si, Gang Sun, Nitin Nitin
Bacterial biofilm formation is linked to several infections and foodborne disease outbreaks. To address this challenge, there is an unmet need to develop rechargeable antimicrobial materials that can provide continuous sanitation of contact surfaces especially in the food industry. This study was aimed at evaluating a novel rechargeable antimicrobial polymer formed using PVA-co-PE with halamine functionality to prevent biofilm formation with repeated exposure to high loads of bacteria and organic content and also aid in inactivation of pre-formed biofilms upon contact with this novel material...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28774672/structure-based-protein-engineering-for-thermostable-and-alkaliphilic-enhancement-of-endo-%C3%AE-1-4-xylanase-for-applications-in-pulp-bleaching
#2
Katewadee Boonyapakron, Aritsara Jaruwat, Benjamas Liwnaree, Thidarat Nimchua, Verawat Champreda, Penchit Chitnumsub
In the pulp bleaching industry, enzymes with robust activity at high pH and temperatures are desirable for facilitating the pre-bleaching process with simplified processing and minimal use of chlorinated compounds. To engineer an enzyme for this purpose, we determined the crystal structure of the Xyn12.2 xylanase, a xylan-hydrolyzing enzyme derived from the termite gut symbiont metagenome, as the basis for structure-based protein engineering to improve Xyn12.2 stability in high heat and alkaline conditions...
July 31, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28656924/comparison-of-intracoronal-bleaching-methods-on-teeth-discolored-by-different-antibiotic-pastes
#3
E Iriboz, B A Öztürk, S Körklü, B Tarcin, Y G Berker, H S Öveçoğlu
AIM: To compare the bleaching efficacy of sodium perborate with different activation methods on crowns discolored by two different antibiotic pastes. MATERIALS AND METHODS: Eighty-five extracted human incisors were prepared to size #30 using ProTaper rotary instruments. After chemomechanical preparation and irrigation procedures, the specimens received triple antibiotic paste (TAP, n = 40), minocycline paste (MP, n = 40), or calcium hydroxide (n = 5, control group) and coronally sealed with temporary filling material...
June 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28606864/suppression-of-nf-%C3%AE%C2%BAb-signal-pathway-by-nlrc3-like-protein-in-stony-coral-acropora-aculeus-under-heat-stress
#4
Zhi Zhou, Yibo Wu, Chengkai Zhang, Can Li, Guangmei Chen, Xiaopeng Yu, Xiaowei Shi, Yanlai Xu, Lingui Wang, Bo Huang
Heat stress is the most common factor for coral bleaching, which has increased both in frequency and severity due to global warming. In the present study, the stony coral Acropora aculeus was subjected to acute heat stress and entire transcriptomes were sequenced via the next generation sequencing platform. Four paired-end libraries were constructed and sequenced in two groups, including a control and a heat stress group. A total of 120,319,751 paired-end reads with lengths of 2 × 100 bp were assembled and 55,021 coral-derived genes were obtained...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28550340/heat-and-bleach-a-cost-efficient-method-for-extracting-microplastics-from-return-activated-sludge
#5
Surya Sujathan, Ann-Kathrin Kniggendorf, Arun Kumar, Bernhard Roth, Karl-Heinz Rosenwinkel, Regina Nogueira
The extraction of plastic microparticles, so-called microplastics, from sludge is a challenging task due to the complex, highly organic material often interspersed with other benign microparticles. The current procedures for microplastic extraction from sludge are time consuming and require expensive reagents for density separation as well as large volumes of oxidizing agents for organic removal, often resulting in tiny sample sizes and thus a disproportional risk of sample bias. In this work, we present an improved extraction method tested on return activated sludge (RAS)...
May 26, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28345029/tidal-heat-pulses-on-a-reef-trigger-a-fine-tuned-transcriptional-response-in-corals-to-maintain-homeostasis
#6
Lupita J Ruiz-Jones, Stephen R Palumbi
For reef-building corals, extreme stress exposure can result in loss of endosymbionts, leaving colonies bleached. However, corals in some habitats are commonly exposed to natural cycles of sub-bleaching stress, often leading to higher stress tolerance. We monitored transcription in the tabletop coral Acropora hyacinthus daily for 17 days over a strong tidal cycle that included extreme temperature spikes, and show that increases in temperature above 30.5°C triggered a strong transcriptional response. The transcriptomic time series data allowed us to identify a set of genes with coordinated expression that were activated only on days with strong tides, high temperature, and large diel pH and oxygen changes...
March 2017: Science Advances
https://www.readbyqxmd.com/read/27980125/warm-preconditioning-protects-against-acute-heat-induced-respiratory-dysfunction-and-delays-bleaching-in-a-symbiotic-sea-anemone
#7
Thomas D Hawkins, Mark E Warner
Preconditioning to non-stressful warming can protect some symbiotic cnidarians against the high temperature-induced collapse of their mutualistic endosymbiosis with photosynthetic dinoflagellates (Symbiodinium spp.), a process known as bleaching. Here, we sought to determine if such preconditioning is underpinned by differential regulation of aerobic respiration. We quantified in vivo metabolism and mitochondrial respiratory enzyme activity in the naturally symbiotic sea anemone Exaiptasia pallida preconditioned to 30°C for>7 weeks as well as anemones kept at 26°C...
December 15, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27920339/identification-of-a-chlorophyll-dephytylase-involved-in-chlorophyll-turnover-in-arabidopsis
#8
Yao-Pin Lin, Meng-Chen Wu, Yee-Yung Charng
Chlorophyll turns over in green organs during photosystem repair and is salvaged via de- and rephytylation, but the enzyme involved in dephytylation is unknown. We have identified an Arabidopsis thaliana thylakoid protein with a putative hydrolase domain that can dephytylate chlorophyll in vitro and in vivo. The corresponding locus, CHLOROPHYLL DEPHYTYLASE1 (CLD1), was identified by mapping a semidominant, heat-sensitive, missense allele (cld1-1). CLD1 is conserved in oxygenic photosynthetic organisms, sharing structural similarity with pheophytinase, which functions in chlorophyll breakdown during leaf senescence...
December 2016: Plant Cell
https://www.readbyqxmd.com/read/27870065/photoacclimatory-and-photoprotective-responses-to-cold-versus-heat-stress-in-high-latitude-reef-corals
#9
Stefanie Pontasch, Paul L Fisher, Thomas Krueger, Sophie Dove, Ove Hoegh-Guldberg, William Leggat, Simon K Davy
Corals at the world's southernmost coral reef of Lord Howe Island (LHI) experience large temperature and light fluctuations and need to deal with periods of cold temperature (<18°C), but few studies have investigated how corals are able to cope with these conditions. Our study characterized the response of key photophysiological parameters, as well as photoacclimatory and photoprotective pigments (chlorophylls, xanthophylls, and β-carotene), to short-term (5-d) cold stress (~15°C; 7°C below control) in three LHI coral species hosting distinct Symbiodinium ITS2 types, and compared the coral-symbiont response to that under elevated temperature (~29°C; 7°C above control)...
November 21, 2016: Journal of Phycology
https://www.readbyqxmd.com/read/27502580/extremely-heat-tolerant-photo-symbiosis-in-a-shallow-marine-benthic-foraminifera
#10
C Schmidt, D Titelboim, J Brandt, B Herut, S Abramovich, A Almogi-Labin, M Kucera
Bleaching, the loss of algal symbionts, occurs in marine photosymbiotic organisms at water temperatures minimally exceeding average summer SST (sea surface temperatures). Pre-adaptation allows organisms to persist under warmer conditions, providing the tolerance can be carried to new habitats. Here we provide evidence for the existence of such adaptation in the benthic foraminifera Pararotalia calcariformata. This species occurs at a thermally polluted site in the Mediterranean, where water temperatures reach a maxima daily average of 36 °C during the summer...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27460544/the-regulation-of-thermal-stress-induced-apoptosis-in-corals-reveals-high-similarities-in-gene-expression-and-function-to-higher-animals
#11
Hagit Kvitt, Hanna Rosenfeld, Dan Tchernov
Recent studies suggest that controlled apoptotic response provides an essential mechanism, enabling corals to respond to global warming and ocean acidification. However, the molecules involved and their functions are still unclear. To better characterize the apoptotic response in basal metazoans, we studied the expression profiles of selected genes that encode for putative pro- and anti-apoptotic mediators in the coral Stylophora pistillata under thermal stress and bleaching conditions. Upon thermal stress, as attested by the elevation of the heat-shock protein gene HSP70's mRNA levels, the expression of all studied genes, including caspase, Bcl-2, Bax, APAF-1 and BI-1, peaked at 6-24 h of thermal stress (hts) and declined at 72 hts...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27069783/gene-expression-profiles-during-short-term-heat-stress-branching-vs-massive-scleractinian-corals-of-the-red-sea
#12
Keren Maor-Landaw, Oren Levy
It is well-established that there is a hierarchy of susceptibilities amongst coral genera during heat-stress. However, molecular mechanisms governing these differences are still poorly understood. Here we explored if specific corals possessing different morphologies and different susceptibilities to heat stress may manifest varied gene expression patterns. We examined expression patterns of seven genes in the branching corals Stylophora pistillata and Acropora eurystoma and additionally in the massive robust coral, Porites sp...
2016: PeerJ
https://www.readbyqxmd.com/read/26977891/toward-plastic-smart-windows-optimization-of-indium-tin-oxide-electrodes-for-the-synthesis-of-electrochromic-devices-on-polycarbonate-substrates
#13
Marco Laurenti, Stefano Bianco, Micaela Castellino, Nadia Garino, Alessandro Virga, Candido F Pirri, Pietro Mandracci
Plastic smart windows are becoming one of the key elements in view of the fabrication of inexpensive, lightweight electrochromic (EC) devices to be integrated in the new generation of high-energy-efficiency buildings and automotive applications. However, fabricating electrochromic devices on polymer substrates requires a reduction of process temperature, so in this work we focus on the development of a completely room-temperature deposition process aimed at the preparation of ITO-coated polycarbonate (PC) structures acting as transparent and conductive plastic supports...
March 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/26869375/-super-quenching-state-protects-symbiodinium-from-thermal-stress-implications-for-coral-bleaching
#14
Chavdar Slavov, Verena Schrameyer, Michael Reus, Peter J Ralph, Ross Hill, Claudia Büchel, Anthony W D Larkum, Alfred R Holzwarth
The global rise in sea surface temperatures causes regular exposure of corals to high temperature and high light stress, leading to worldwide disastrous coral bleaching events (loss of symbiotic dinoflagellates (Symbiodinium) from reef-building corals). Our picosecond chlorophyll fluorescence experiments on cultured Symbiodinium clade C cells exposed to coral bleaching conditions uncovered the transformations of the alga's photosynthetic apparatus (PSA) that activate an extremely efficient non-photochemical "super-quenching" mechanism...
June 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26822785/a-multi-substrate-approach-for-functional-metagenomics-based-screening-for-hemi-cellulases-in-two-wheat-straw-degrading-microbial-consortia-unveils-novel-thermoalkaliphilic-enzymes
#15
Mukil Maruthamuthu, Diego Javier Jiménez, Patricia Stevens, Jan Dirk van Elsas
BACKGROUND: Functional metagenomics is a promising strategy for the exploration of the biocatalytic potential of microbiomes in order to uncover novel enzymes for industrial processes (e.g. biorefining or bleaching pulp). Most current methodologies used to screen for enzymes involved in plant biomass degradation are based on the use of single substrates. Moreover, highly diverse environments are used as metagenomic sources. However, such methods suffer from low hit rates of positive clones and hence the discovery of novel enzymatic activities from metagenomes has been hampered...
January 28, 2016: BMC Genomics
https://www.readbyqxmd.com/read/26782747/an-in-depth-characterization-of-the-entomopathogenic-strain-bacillus-pumilus-15-1-reveals-that-it-produces-inclusion-bodies-similar-to-the-parasporal-crystals-of-bacillus-thuringiensis
#16
Diana C Garcia-Ramon, C Alfonso Molina, Antonio Osuna, Susana Vílchez
In the present work, the local isolate Bacillus pumilus 15.1 has been morphologically and biochemically characterized in order to gain a better understanding of this novel entomopathogenic strain active against Ceratitis capitata. This strain could represent an interesting biothechnological tool for the control of this pest. Here, we report on its nutrient preferences, extracellular enzyme production, motility mechanism, biofilm production, antibiotic suceptibility, natural resistance to chemical and physical insults, and morphology of the vegetative cells and spores...
April 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26687892/characterization-of-a-purified-thermostable-xylanase-from-caldicoprobacter-algeriensis-sp-nov-strain-th7c1-t
#17
Bouanane-Darenfed Amel, Boucherba Nawel, Bouacem Khelifa, Gagaoua Mohammed, Joseph Manon, Kebbouche-Gana Salima, Nateche Farida, Hacene Hocine, Ollivier Bernard, Cayol Jean-Luc, Fardeau Marie-Laure
The present study investigates the purification and biochemical characterization of an extracellular thermostable xylanase (called XYN35) from Caldicoprobacter algeriensis sp. nov., strain TH7C1(T), a thermophilic, anaerobic strain isolated from the hydrothermal hot spring of Guelma (Algeria). The maximum xylanase activity recorded after 24 h of incubation at 70 °C and in an optimized medium containing 10 g/L mix birchwood- and oats spelt-xylan was 250 U/mL. The pure protein was obtained after heat treatment (1 h at 70 °C), followed by sequential column chromatographies on Sephacryl S-200 gel filtration and Mono-S Sepharose anion-exchange...
January 2016: Carbohydrate Research
https://www.readbyqxmd.com/read/26554539/mechanochemical-synthesis-and-antioxidant-activity-of-curcumin-templated-azoles
#18
Daisy R Sherin, Kallikat N Rajasekharan
A solvent-free, mechanochemical method for the synthesis of curcumin (1) derived 3,5-bis(styryl)pyrazoles and 3,5-bis(styryl)isoxazole (2a-g) at room temperature, with very short reaction time, is reported. Such earlier structural modifications of curcumin, at its β-diketone unit by transforming it into an isosteric pyrazole or isoxazole unit, required prolonged heating. The evaluation of the antioxidant activity of these compounds, based on DPPH, FRAP, and β-carotene bleaching assays, showed that several of these azoles are better antioxidants than curcumin, with the isoxazole derivative 2g being overall the best...
December 2015: Archiv der Pharmazie
https://www.readbyqxmd.com/read/26002109/purification-and-biochemical-characterization-of-two-detergent-stable-serine-alkaline-proteases-from-streptomyces-sp-strain-ah4
#19
Souraya Boulkour Touioui, Nadia Zaraî Jaouadi, Hadjira Boudjella, Fatma Zohra Ferradji, Mouna Belhoul, Hatem Rekik, Abdelmalek Badis, Samir Bejar, Bassem Jaouadi
Streptomyces sp. strain AH4 exhibited a high ability to produce two extracellular proteases when cultured on a yeast malt-extract (ISP2)-casein-based medium. Pure proteins were obtained after heat treatment (30 min at 70 °C) and ammonium sulphate fractionation (30-60 %), followed by size exclusion HPLC column. Matrix assisted laser desorption ionization-time of flight mass spectrometry analysis revealed that the purified enzymes (named SAPS-P1 and SAPS-P2) were monomers with molecular masses of 36,417.13 and 21,099...
July 2015: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/25874258/laser-teeth-bleaching-evaluation-of-eventual-side-effects-on-enamel-and-the-pulp-and-the-efficiency-in-vitro-and-in-vivo
#20
REVIEW
Roeland Jozef Gentil De Moor, Jeroen Verheyen, Peter Verheyen, Andrii Diachuk, Maarten August Meire, Peter Jozef De Coster, Mieke De Bruyne, Filip Keulemans
Light and heat increase the reactivity of hydrogen peroxide. There is no evidence that light activation (power bleaching with high-intensity light) results in a more effective bleaching with a longer lasting effect with high concentrated hydrogen peroxide bleaching gels. Laser light differs from conventional light as it requires a laser-target interaction. The interaction takes place in the first instance in the bleaching gel. The second interaction has to be induced in the tooth, more specifically in the dentine...
2015: TheScientificWorldJournal
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