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Light activated bleaching

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https://www.readbyqxmd.com/read/27920339/identification-of-chlorophyll-dephytylase-involved-in-chlorophyll-turnover-in-arabidopsis
#1
Yao-Pin Lin, Meng-Chen Wu, Yee-Yung Charng
Chlorophyll turns over in green organs during photosystem repair and is salvaged via de- and re-phytylation, but the enzyme involved in de-phytylation is unknown. We have identified an Arabidopsis thaliana thylakoid protein with a putative hydrolase domain that can dephytylate chlorophyll in vitro and in vivo. The corresponding locus, CHLOROPHYLL DEPHYTYLASE1 (CLD1), was identified by mapping a semi-dominant, heat-sensitive, missense allele (cld1-1). CLD1 is conserved in oxygenic photosynthetic organisms, sharing structural similarity with pheophytinase, which functions in chlorophyll breakdown during leaf senescence...
December 5, 2016: Plant Cell
https://www.readbyqxmd.com/read/27892835/effect-of-adhesive-restoration-and-bleaching-technique-on-the-concentration-of-hydrogen-peroxide-in-the-pulp-chamber
#2
V Cavalli, B G Silva, S B Berger, G Abuna, F C Marson, Cpm Tabchoury, M Giannini
This study aimed to quantify the concentration of hydrogen peroxide into the pulp chamber in the presence or absence of adhesive enamel restorations and to analyze the resin-dentin interface of bleached groups. Bovine incisors (120) were randomly divided into three groups according to enamel treatment (n=40 each): (1) enamel without restoration (control); (2) enamel cavities (3 mm diameter × 1.5 mm depth) restored with a silorane-based (SB) system; or (3) enamel cavities (3 mm diameter × 1.5 mm depth) restored with a dimethacrylate-based (DB) system...
November 28, 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27870065/photoacclimatory-and-photoprotective-responses-to-cold-versus-heat-stress-in-high-latitude-reef-corals
#3
Stefanie Pontasch, Paul L Fisher, Thomas Krueger, Sophie Dove, William Leggat, Ove Hoegh-Guldberg, Simon K Davy
Corals at the world's southernmost coral reef of Lord Howe Island (LHI) experience large temperature and light fluctuations and need to deal with periods of cold temperature (< 18 °C), but few studies have investigated how corals are able to cope with these conditions. Our study characterized the response of key photophysiological parameters, as well as photoacclimatory and photoprotective pigments (chlorophylls, xanthophylls and β-carotene), to short-term (5-day) cold stress (~ 15 °C; 7 °C below control) in three LHI coral species hosting distinct Symbiodinium ITS2 types, and compared the coral-symbiont response to that under elevated temperature (~ 29 °C; 7 °C above control)...
November 21, 2016: Journal of Phycology
https://www.readbyqxmd.com/read/27837096/downregulation-of-the-alternative-sigma-factor-sigj-confers-a-photoprotective-phenotype-to-anabaena-pcc-7120
#4
Amit Srivastava, Klaus Brilisauer, Ashutosh K Rai, Anand Ballal, Karl Forchhammer, Anil K Tripathi
Alternative sigma factors belonging to the Group 3 are thought to play an important role in the adaptation of cyanobacteria to environmental challenges by altering expression of genes needed for coping with such stresses. In this study, the role of an alternative sigma factor, SigJ, was analyzed in the filamentous nitrogen-fixing cyanobacterium, Anabaena sp. PCC 7120 by knocking down the expression of the sigJ gene (alr0277) employing an anti-sense RNA-mediated approach. In the absence of any stress, the knock-down (KD0277) or the wild-type strain both grew similarly...
November 10, 2016: Plant & Cell Physiology
https://www.readbyqxmd.com/read/27811449/bond-strength-of-resin-composite-to-light-activated-bleached-enamel
#5
T Yavuz, O Y Ozyilmaz, A N Ozturk, F Aykent
OBJECTIVES: This study evaluated the microtensile bond strength (μTBS) of a resin composite bonded to bleached enamel as a function of bleaching conditions. MATERIALS AND METHODS: The whiteness hydrogen peroxide (HP) bleaching agent containing 35% HP was applied to the central incisors' facial enamel surface and irradiated as follows: No treatment (G1; control); no light (G2); light-emitting diode, the 40s (G3); diode laser, the 20s (G4); and neodymium:yttrium aluminum garnet laser, 20s (G5)...
November 2016: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/27747220/resin-bonding-of-self-etch-adhesives-to-bovine-dentin-bleached-from-pulp-chamber
#6
Akiko Haruyama, Atsushi Kameyama, Junji Kato, Shinji Takemoto, Yutaka Oda, Eiji Kawada, Toshiyuki Takahashi, Masahiro Furusawa
This study evaluated the microtensile bond strength (μTBS) of 1-step self-etch adhesives (1-SEAs) and 2-step self-etch adhesives (2-SEAs) to pulp chamber dentin immediately after bleaching with 2 types of common bleaching techniques. Pulp chamber dentin of bovine teeth was bleached using 30% hydrogen peroxide (H2O2) solution with quartz-tungsten-halogen light-curing unit (Group 1) and 3.5% H2O2-containing titanium dioxide (TiO2) (Pyrenees®) activated with 405-nm violet diode laser for 15 min (Group 2). Unbleached specimens were placed in distilled water for 15 min and used as controls...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27701496/post-bleaching-sensitivity-in-patients-with-sickle-cell-disease
#7
Guacyra M Lisboa, Verónica L Guedes, Maria do R Ml Luna, Américo M Carneiro-Jr, Roberto C Stegun
Sickle cell disease (SCD) is a monogenic disease that affects millions of people worldwide. This study analyzed the effectiveness of bleaching and tooth sensitivity after in-office bleaching in patients with SCD. Forty volunteers were randomly assigned to four groups of 10 patients each (five with the SCD and five healthy controls) and treated using in-office bleaching with 35% hydrogen peroxide and different light activation protocols. No statistically significant difference was observed with relation to presence of tooth sensitivity, with or without use of a source of light for peroxide activation, and all bleaching therapies were effective, regardless of the technique employed and the presence/absence of sickle cell disease...
April 2016: Acta Odontológica Latinoamericana: AOL
https://www.readbyqxmd.com/read/27630493/impact-of-hydrogen-peroxide-activated-by-lighting-emitting-diode-laser-system-on-enamel-color-and-microhardness-an-in-situ-design
#8
Ana Bárbara Araújo Loiola, Aline Evangelista Souza-Gabriel, Renata Siqueira Scatolin, Silmara Aparecida Milori Corona
BACKGROUND: Hydrogen peroxide (HP) at lower concentration can provide less alteration on enamel surface and when combined with laser therapy, could decrease tooth sensitivity. This in situ study evaluated the influence of 15% and 35% HP gel activated by lighting-emitting diode (LED)/laser light for in-office tooth bleaching. MATERIALS AND METHODS: Forty-four bovine enamel slabs were polished and subjected to surface microhardness (load of 25 g for 5 s). The specimens were placed in intraoral palatal devices of 11 volunteers (n = 11)...
July 2016: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/27575620/advanced-cellulose-fibers-for-efficient-immobilization-of-enzymes
#9
Iris Beatriz Vega Erramuspe, Elnaz Fazeli, Tuomas Näreoja, Jani Trygg, Pekka Hänninen, Thomas Heinze, Pedro Fardim
Biocatalytic pulp fibers were prepared using surface functionalization of bleached kraft pulp with amino groups (F) and further immobilization of a cross-linked glucose oxidase (G*) from Aspergillus niger. The cross-linked enzymes (G*) were characterized using X-ray spectroscopy, Fourier transform infrared spectroscopy, dynamic scanning calorimetry, and dynamic light scattering. According to standard assays, the G* content on the resulting fibers (FG*) was of 11 mg/g of fiber, and enzyme activity was of 215 U/g...
October 10, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27525071/comparative-clinical-and-psychosocial-benefits-of-tooth-bleaching-different-light-activation-of-a-38-peroxide-gel-in-a-preliminary-case-control-study
#10
Angelo Calderini, Simona Sciara, Chiara Semeria, Giuseppe Pantaleo, Elisabetta Polizzi
Tooth bleaching is a widespread dental treatment with important psychosocial antecedents and outcomes involved. In the activation of in-office bleaching agents, a selective light radiation, that is, a diode laser seems to be a positive choice to decrease the time of bleaching without surface modification and with no residual tooth sensitivity for maximum effect and minimal clinical and psychological side effects.
August 2016: Clinical Case Reports
https://www.readbyqxmd.com/read/27517329/stimulated-emission-depletion-nanoscopy-reveals-time-course-of-human-immunodeficiency-virus-proteolytic-maturation
#11
Janina Hanne, Fabian Göttfert, Jiří Schimer, Maria Anders-Össwein, Jan Konvalinka, Johann Engelhardt, Barbara Müller, Stefan W Hell, Hans-Georg Kräusslich
Concomitant with human immunodeficiency virus type 1 (HIV-1) budding from a host cell, cleavage of the structural Gag polyproteins by the viral protease (PR) triggers complete remodeling of virion architecture. This maturation process is essential for virus infectivity. Electron tomography provided structures of immature and mature HIV-1 with a diameter of 120-140 nm, but information about the sequence and dynamics of structural rearrangements is lacking. Here, we employed super-resolution STED (stimulated emission depletion) fluorescence nanoscopy of HIV-1 carrying labeled Gag to visualize the virion architecture...
September 27, 2016: ACS Nano
https://www.readbyqxmd.com/read/27433554/hydrogen-peroxide-diffusion-with-and-without-light-activation
#12
Carmen Llena, Leopoldo Forner, María Vazquez
OBJECTIVE: The aim of this study was to assess the dental bleaching efficacy of 37.5% hydrogen peroxide (HP), with and without light activation, in HP-exposed and unexposed areas. METHOD: 28 bovine teeth were selected and divided into two groups (n = 14). Crowns were detached and stained with tea. The gingival half was covered with a gingival barrier. In the incisal half, 37.5% HP (Pola Office+, SDI) was applied three times, with a 1-week interval between applications...
2016: International Journal of Esthetic Dentistry
https://www.readbyqxmd.com/read/27358455/effect-of-rhodopsin-phosphorylation-on-dark-adaptation-in-mouse-rods
#13
Justin Berry, Rikard Frederiksen, Yun Yao, Soile Nymark, Jeannie Chen, Carter Cornwall
UNLABELLED: Rhodopsin is a prototypical G-protein-coupled receptor (GPCR) that is activated when its 11-cis-retinal moiety is photoisomerized to all-trans retinal. This step initiates a cascade of reactions by which rods signal changes in light intensity. Like other GPCRs, rhodopsin is deactivated through receptor phosphorylation and arrestin binding. Full recovery of receptor sensitivity is then achieved when rhodopsin is regenerated through a series of steps that return the receptor to its ground state...
June 29, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27352147/a-single-blind-randomized-trial-about-the-effect-of-hydrogen-peroxide-concentration-on-light-activated-bleaching
#14
A P Mena-Serrano, E Garcia, I Luque-Martinez, Rhm Grande, A D Loguercio, A Reis
OBJECTIVE: To compare the bleaching efficacy and tooth sensitivity (TS) of two hydrogen peroxide (HP) concentrations (20% and 35%) used for in-office bleaching associated or not with a light-emitting diode (LED)/laser light activation. METHOD: Seventy-seven patients with a right maxillary canine darker than A3 were selected for this single-blind randomized trial. The participants were distributed in four groups: bleaching with 35% HP, 35% HP + LED/laser, 20% HP, and 20% HP + LED/laser...
June 28, 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27337413/acridinium-ester-functionalized-carbon-nanomaterials-general-synthesis-strategy-and-outstanding-chemiluminescence
#15
Zhili Han, Fang Li, Jiangnan Shu, Lingfeng Gao, Xiaoying Liu, Hua Cui
In this work, three different kinds of acridinium ester (AE)-functionalized carbon nanomaterials, including AE-functionalized carbon nanoparticles (AE-CNPs), AE-functionalized graphene oxide (AE-GO), and AE-functionalized multiwalled carbon nanotubes (AE-MCNTs), were synthesized for the first time via a simple, general, and noncovalent strategy. AE molecules were assembled on the surface of carbon nanomaterials by electrostatic interaction, π-π stacking interaction, and amide bond. The synthesized AE-CNPs, AE-GO, and AE-MCNTs with 5...
July 13, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27243936/photoactivation-of-luminescent-centers-in-single-sio2-nanoparticles
#16
Luigi Tarpani, Daja Ruhlandt, Loredana Latterini, Dirk Haehnel, Ingo Gregor, Jörg Enderlein, Alexey I Chizhik
Photobleaching of fluorophores is one of the key problems in fluorescence microscopy. Overcoming the limitation of the maximum number of photons, which can be detected from a single emitter, would allow one to enhance the signal-to-noise ratio and thus the temporal and spatial resolution in fluorescence imaging. It would be a breakthrough for many applications of fluorescence spectroscopy, which are unachievable up to now. So far, the only approach for diminishing the effect of photobleaching has been to enhance the photostability of an emitter...
July 13, 2016: Nano Letters
https://www.readbyqxmd.com/read/27217641/evaluation-of-bleaching-efficacy-of-37-5-hydrogen-peroxide-on-human-teeth-using-different-modes-of-activations-an-in-vitro-study
#17
Neha Bhutani, Bhuvan Shome Venigalla, Jaya Prakash Patil, Thakur Veerandar Singh, Sistla Venkata Jyotsna, Abhilasha Jain
INTRODUCTION: The aim of this in vitro study is to evaluate the role of light and laser sources in the bleaching ability of 37.5% H2 O2 on extracted human teeth. MATERIALS AND METHODS: About 30 caries-free single-rooted maxillary central incisors were used for the study. Specimens were prepared by sectioning the crown portion of teeth mesiodistally, and labial surface was used for the study. Specimens were then immersed in coffee solution for staining. Color of each tooth was analyzed using Shadestar, a digital shademeter...
May 2016: Journal of Conservative Dentistry: JCD
https://www.readbyqxmd.com/read/27119761/evaluation-of-temperature-increase-during-in-office-bleaching
#18
Rafael Francisco Lia Mondelli, Ana Flávia Soares, Eugenio Gabriel Kegler Pangrazio, Linda Wang, Sergio Kiyoshi Ishikiriama, Juliana Fraga Soares Bombonatti
The use of light sources in the bleaching process reduces the time required and promotes satisfactory results. However, these light sources can cause an increase in the pulp temperature. Objective The purpose of the present study was to measure the increase in intrapulpal temperature induced by different light-activated bleaching procedures with and without the use of a bleaching gel. Material and Methods A human maxillary central incisor was sectioned 2 mm below the cementoenamel junction. A K-type thermocouple probe was introduced into the pulp chamber...
April 2016: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/27114888/changes-to-coral-health-and-metabolic-activity-under-oxygen-deprivation
#19
James W A Murphy, Robert H Richmond
On Hawaiian reefs, the fast-growing, invasive algae Gracilaria salicornia overgrows coral heads, restricting water flow and light, thereby smothering corals. Field data shows hypoxic conditions (dissolved oxygen (DO2) < 2 mg/L) occurring underneath algal mats at night, and concurrent bleaching and partial tissue loss of shaded corals. To analyze the impact of nighttime oxygen-deprivation on coral health, this study evaluated changes in coral metabolism through the exposure of corals to chronic hypoxic conditions and subsequent analyses of lactate, octopine, alanopine, and strombine dehydrogenase activities, critical enzymes employed through anaerobic respiration...
2016: PeerJ
https://www.readbyqxmd.com/read/27107335/antimicrobial-photodynamic-effect-of-extracts-and-oxoaporphine-alkaloid-isomoschatoline-from-guatteria-blepharophylla
#20
Nathalia Luiza Andreazza, Caroline Caramano de Lourenço, Álvaro José Hernandez-Tasco, Maria Lúcia B Pinheiro, Maria Élida Alves Stefanello, Emmanoel Vilaça Costa, Marcos José Salvador
Photodynamic Therapy, a tumor therapy idealized at the beginning of the last century, emerges nowadays as a promising treatment alternative against infectious diseases. In this study we report a bioguided study of Guatteria blepharophylla phytoderivatives for antimicrobial PDT. Crude extracts and fraction from the species bark were obtained and further fractionated for substances isolation. All samples were evaluated in relation to their photophysical (absorbance and fluorescence) and photochemical properties (1,3-DPBF bleaching method)...
July 2016: Journal of Photochemistry and Photobiology. B, Biology
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