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https://www.readbyqxmd.com/read/29331291/global-microrna-profiling-in-human-bone-marrow-skeletal-stromal-or-mesenchymal-stem-cells-identified-candidates-for-bone-regeneration
#1
Chi-Chih Chang, Morten T Venø, Li Chen, Nicholas Ditzel, Dang Q S Le, Philipp Dillschneider, Moustapha Kassem, Jørgen Kjems
Bone remodeling and regeneration are highly regulated multistep processes involving posttranscriptional regulation by microRNAs (miRNAs). Here, we performed a global profiling of differentially expressed miRNAs in bone-marrow-derived skeletal cells (BMSCs; also known as stromal or mesenchymal stem cells) during in vitro osteoblast differentiation. We functionally validated the regulatory effects of several miRNAs on osteoblast differentiation and identified 15 miRNAs, most significantly miR-222 and miR-423, as regulators of osteoblastogenesis...
December 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29330462/mir-143-3p-inhibits-the-proliferation-migration-and-invasion-in-osteosarcoma-by-targeting-fosl2
#2
Xiangran Sun, Guo Dai, Ling Yu, Qingzhu Hu, Jingteng Chen, Weichun Guo
Osteosarcoma (OS) is the most common type of primary malignant bone tumor and mainly occurs in children and adolescent. Because of its early migration and invasion, OS has a poor prognosis. It has been reported that mircoRNAs (miRNAs) play a crucial role in the occurrence and development of multiple tumors. In this study, we identified the aberrant-expression of miR-143-3p in osteosarcoma and examined the role of miR-143-3p in OS development. Further, we searched the miR-143-3p target gene and verified its accuracy by luciferase experiments...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29328417/microrna-129-5p-suppresses-cell-proliferation-migration-and-invasion-via-targeting-rock1-in-osteosarcoma
#3
Chaofan Han, Wenbo Wang
Osteosarcoma (OS) is the most common primary malignancy of the bone in teenagers and accounts for 20‑35% of all malignant primary bone tumors. Increasing evidence shows that microRNAs (miRNAs) are abnormally expressed in several types of human cancer. miRNAs are necessary to maintain the malignant phenotype of cancer cells and can function as either tumor suppressors or oncogenes. The present study aimed to measure the expression of miRNA‑129‑5p (miR‑129‑5p) in OS, determine the effects of miR‑129‑5p on the malignant behaviors of OS cells, and elucidate the molecular mechanism underlying the oncogenesis and progression of OS...
January 4, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29303912/transforming-growth-factor-%C3%AE-and-bone-morphogenetic-protein-2-regulation-of-microrna-200-family-in-chronic-pancreatitis
#4
Peter Yu, Ka Liu, Xuxia Gao, Harry Karmouty-Quintana, Jennifer M Bailey, Yanna Cao, Tien C Ko
OBJECTIVES: To investigate regulation of microRNA (miR)-200 family (a, b, c, 141, and 429) in chronic pancreatitis (CP). This was accomplished by examining miR-200 family levels in a mouse model in vivo and their regulation in pancreatic cells in vitro. METHODS: Chronic pancreatitis was induced by cerulein for 4 weeks (50 μg/kg, 5 hourly intraperitoneal injections/day, and 3 days/week). Control mice received normal saline. The pancreata were harvested for fibrosis assessment by Sirius red staining and for miRNA, collagen, and fibronectin levels by quantitative PCR...
January 5, 2018: Pancreas
https://www.readbyqxmd.com/read/29296725/replenishing-exosomes-from-older-bone-marrow-stromal-cells-with-mir-340-inhibits-myeloma-related-angiogenesis
#5
Tomohiro Umezu, Satoshi Imanishi, Kenko Azuma, Chiaki Kobayashi, Seiichiro Yoshizawa, Kazuma Ohyashiki, Junko H Ohyashiki
The study of bone marrow stromal cells (BMSCs) and the exosomes they secrete is considered promising for cancer therapy. However, little is known about the effect of donor age on BMSCs. In the present study, we investigated the therapeutic potential of BMSC exosomes derived from donors of different ages using an in vivo model of hypoxic bone marrow in multiple myeloma (MM). We found that donor age was strongly related to senescent changes in BMSCs. Exosomes derived from young BMSCs significantly inhibited MM-induced angiogenesis in Matrigel plugs...
May 23, 2017: Blood Advances
https://www.readbyqxmd.com/read/29288704/the-mirn23a-and-mirn23b-microrna-clusters-are-necessary-for-proper-hematopoietic-progenitor-cell-production-and-differentiation
#6
Jeffrey L Kurkewich, Austin Boucher, Nathan Klopfenstein, Ramdas Baskar, Reuben Kapur, Richard Dahl
Mice deficient for microRNA (miRNA) cluster mirn23a exhibit increased B lymphopoiesis at the expense of myelopoiesis while hematopoietic stem and progenitor (HSPC) populations are unchanged. Mammals possess a paralogous mirn23b gene that can give rise to 3 mature miRNAs (miRs -23b, 24-1, and -27b) that have identical seed/ mRNA targeting sequences to their mirn23a counterparts. To assess whether compound deletion of mirn23a and mirn23b exacerbates the hematopoietic phenotype observed in mirn23a-/- mice, we generated a compound mirn23a-/-mirn23bfl/fl: Mx1-Cre conditional knockout mouse and assayed hematopoietic development after excision of mirn23b...
December 27, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/29288578/mir-96-regulates-bone-metabolism-by-targeting-osterix
#7
Hua Liu, Qing Liu, Xian-Ping Wu, Hong-Bo He, Lei Fu
MicroRNAs (miRNAs) play important roles in bone metabolism and aging. Here we show that miR-96 was markedly up-regulated in serum of elderly patients with osteoporosis by miRNA microarray analysis and qRT-PCR. Moreover miR-96 was also up-regulated in bone marrow mesenchymal stem cells (BMSCs) of aged humans and mice. Our results show that the over-expression of miR-96 reduced osteogenic differentiation of BMSCs, whereas the inhibition of miR-96 increased osteogenic differentiation of BMSCs. At the molecular level, miR-96 regulated osteogenesis by targeting osterix...
December 30, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29288400/anti-inflammatory-and-antiresorptive-effects-of-calendula-officinalis-on-inflammatory-bone-loss-in-rats
#8
Joanna Trycia M Alexandre, Luzia Hermínia Teixeira Sousa, Mario Roberto Pontes Lisboa, Flávia A C Furlaneto, Danielle Rocha do Val, Mirna Marques, Hellíada C Vasconcelos, Iracema Matos de Melo, Renata Leitão, Gerly Anne Castro Brito, Paula Goes
OBJECTIVE: The aim of this work was to evaluate the anti-inflammatory and antiresorptive effects of Calendula officinalis (CLO) on alveolar bone loss (ABL) in rats. MATERIAL AND METHODS: Male Wistar rats were subjected to ABL by ligature with nylon thread around the second upper left molar. The contralateral hemimaxillae were used as control. Rats received saline solution (SAL) or CLO (10, 30, or 90 mg/kg) 30 min before ligature and daily until the 11th day. The maxillae were removed and prepared for macroscopic, radiographic, micro-tomographic, histopathologic, histometric analysis, and immunohistochemical localization of receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG)...
December 29, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29285077/identification-of-micrornas-in-human-circulating-monocytes-of-postmenopausal-osteoporotic-mexican-mestizo-women-a-pilot-study
#9
Rogelio F Jiménez-Ortega, Eric G Ramírez-Salazar, Alma Y Parra-Torres, Said A Muñoz-Montero, Claudia Rangel-Escareňo, Ivan Salido-Guadarrama, Mauricio Rodriguez-Dorantes, Manuel Quiterio, Jorge Salmerón, Rafael Velázquez-Cruz
MicroRNAs (miRNAs or miRs) are a class of short non-coding RNAs that serve an important regulatory role in living organisms. These molecules are associated with multiple biological processes and are potential biomarkers in multiple diseases. The present study aimed to further identify miRNAs that are differentially expressed in circulating monocytes (CMCs) from postmenopausal Mexican-Mestizo women. Microarray analyses of monocytes using Affymetrix miRNA 4.0 and Human Genome U133 Plus 2.0 arrays were performed in 6 normal and 6 osteoporotic women, followed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29273522/matrix-metalloproteinase-13-a-special-focus-on-its-regulation-by-signaling-cascades-and-micrornas-in-bone
#10
REVIEW
Saiganesh S, Saathvika R, Udhaya V, Arumugam B, Vishal M, Selvamurugan N
Bone remodeling is an orchestrated process involving osteoblasts and osteoclasts to maintain mineral homeostasis in the internal milieu, mediated chiefly by matrix metalloproteinases (MMPs). MMP13, one amongst the MMPs plays a premier role in bone remodeling, and mutations in MMP13 have been implicated in various pathologies including cancer and arthritis. Transcriptional activation of MMP13 gene is tightly regulated by several signaling cascade components. Post-transcriptional regulators such as microRNAs (miRNAs) have also been shown to regulate MMP13 expression under physiological and pathological conditions...
December 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29249988/stemodia-maritima-l-extract-decreases-inflammation-oxidative-stress-and-alveolar-bone-loss-in-an-experimental-periodontitis-rat-model
#11
Alrieta H Teixeira, Jordânia M de Oliveira Freire, Luzia H T de Sousa, Antônia T Parente, Nayara A de Sousa, Angela M C Arriaga, Francisca R Lopes da Silva, Iracema M Melo, Igor I Castro da Silva, Karuza M A Pereira, Paula Goes, José J do Nascimento Costa, Gerardo Cristino-Filho, Vicente de Paulo T Pinto, Hellíada V Chaves, Mirna M Bezerra
Periodontitis is very prevalent worldwide and is one of the major causes of tooth loss in adults. About 80% of the worldwide population use medicinal plants for their health care. Stemodia maritima L. (S. maritima) antioxidant and antimicrobial effects in vitro as well as anti-inflammatory properties. Herein, the potential therapeutic effect of S. maritima was assessed in rats subjected to experimental periodontitis (EP). EP was induced in female Wistar rats by nylon thread ligature around 2nd upper left molars for 11 days...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29246528/epigenetics-of-bone-disease
#12
Laetitia Michou
Histone deacetylation, DNA methylation, and micro-RNAs (miRNAs) are the three main epigenetic mechanisms that regulate gene expression. All the physiological processes involved in bone remodeling are tightly regulated by epigenetic factors. This review discusses the main epigenetic modifications seen in tumoral and nontumoral bone diseases, with emphasis on miRNAs. The role for epigenetic modifications of gene expression in the most common bone diseases is illustrated by drawing on the latest publications in the field...
December 12, 2017: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/29234953/involvement-of-microrna-23b-in-tnf-%C3%AE-reduced-bmsc-osteogenic-differentiation-via-targeting-runx2
#13
Lin Deng, Guoli Hu, Lei Jin, Chenglong Wang, Hongwen Niu
Elucidation of the molecular mechanism governing bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation is of great importance for improving the treatment of osteoporosis. TNF-α is a well-known inhibitory factor during osteogenic differentiation of BMSCs. In our experiment, we consistently observed that TNF-α significantly inhibited BMSC osteogenic differentiation, which was partially rescued by BAY 11-7082 (NF-κB inhibitor). In this study, we examined the potential roles of microRNAs (miRNAs) involved in TNF-α-mediated reduction of BMSC osteogenesis...
December 12, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29230197/exosome-mediated-genetic-information-transfer-a-missing-piece-of-osteoblast-osteoclast-communication-puzzle
#14
Pengbin Yin, Houchen Lv, Yi Li, Yuan Deng, Licheng Zhang, Peifu Tang
The skeletal system functions and maintains itself based on communication between cells of diverse origins, especially between osteoblasts (OBs) and osteoclasts (OCs), accounting for bone formation and resorption, respectively. Previously, protein-level information exchange has been the research focus, and this has been discussed in detail. The regulative effects of microRNAs (miRNAs) on OB and OC ignite the question as to whether genetic information could be transferred between bone cells. Exosomes, extracellular membrane vesicles 30-100 nm in diameter, have recently been demonstrated to transfer functional proteins, mRNAs, and miRNAs, and serve as mediators of intercellular communication...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29228567/biomarker-significance-of-plasma-and-tumor-mir-21-mir-221-and-mir-106a-in-osteosarcoma
#15
Manjula Nakka, Wendy Allen-Rhoades, Yiting Li, Aaron J Kelly, Jianhe Shen, Aaron M Taylor, Donald A Barkauskas, Jason T Yustein, Irene L Andrulis, Jay S Wunder, Richard Gorlick, Paul S Meltzer, Ching C Lau, Tsz-Kwong Man
Osteosarcoma is the most common malignant bone tumor in children and young adults. Despite the use of surgery and multi-agent chemotherapy, osteosarcoma patients who have a poor response to chemotherapy or develop relapses have a dismal outcome. Identification of biomarkers for active disease may help to monitor tumor burden, detect early relapses, and predict prognosis in these patients. In this study, we examined whether circulating miRNAs can be used as biomarkers in osteosarcoma patients. We performed genome-wide miRNA profiling on a discovery cohort of osteosarcoma and control plasma samples...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29226968/study-of-micrornas-targeted-dvl2-on-the-osteoblasts-differentiation-of-rat-bmscs-in-hyperlipidemia-environment
#16
Xin Huang, Duoduo Li, Zhifeng Wang, Zhenfei Huang, Xiaofei Dong, Chuanhua Li, Jing Lan
Dishevelled 2 (Dvl-2), a key mediator of the wnt/β-catenin signaling pathway, plays critical roles in osteoblasts differentiation in hyperlipidemia environment. In our previous study, we observed a strong correlation between increased dvl2 expression and decreased new bone formation around implants in a rat hyperlipidemia implant surgery model. However, transcriptional regulation of Dvl2 by microRNAs in this process remains unknown. In the current study, we searched in online database and identified four significantly up-regulated miRNAs, miR-21-5p, miR-29c-3p, miR-138-5p and miR-351-5p that could potentially regulate Dvl2...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29226656/peripheral-blood-mir-181-5p-serves-as-a-marker-for-screening-patients-with-osteoarthritis-by-targeting-tnf%C3%AE
#17
Silong Xia, Hua Tian, Lei Fan, Jianying Zheng
BACKGROUND: Increasing evidence has suggested the important role of miRNAs in the progression of osteoarthritis (OA). In the current study, we explored the role of miR-181-5p in the peripheral blood and bone tissues of OA patients for the first time. METHODS: The level of miR-181-5p was evaluated in the peripheral blood and cartilage of OA patients using real time PCR analysis. ROC analysis was performed to explore whether peripheral blood and cartilage miR-181-5p could screen OA patients from normal controls...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29220106/chemoprevention-by-resveratrol-and-pterostilbene-targeting-on-epigenetic-regulation
#18
REVIEW
Pei-Sheng Lee, Yi-Shiou Chiou, Chi-Tang Ho, Min-Hsiung Pan
Epigenetic mechanisms are essential in regulating normal cellular functions and play an important role during the disease developmental stages. However, aberrant epigenetic mechanisms may lead to pathological consequences such as cancer, neurological disorders, bone and skeletal diseases, cardiovascular dysfunction, and metabolic syndrome. The molecular mechanisms of epigenetic modification include DNA methylation, histone modification (acetylation, methylation and phosphorylation), and microRNAs (miRNAs). Unlike genetic modifications, epigenetic states of genes are reversible and can be altered by certain intrinsic and extrinsic factors...
December 8, 2017: BioFactors
https://www.readbyqxmd.com/read/29218094/mir-351-negatively-regulates-osteoblast-differentiation-of-mscs-induced-by-cholesten-3-one-through-targeting-vdr
#19
Qiuke Hou, Yongquan Huang, Yiwen Luo, Bin Wang, Yamei Liu, Rudong Deng, Saixia Zhang, Fengbin Liu, Dongfeng Chen
Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. Overexpression of miR-351 by miR-351 precursor transfection markedly inhibited the expression of osteoblast-specific genes, such as alkaline phosphatase (ALP), collagen type II, osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), which consequently decreased a number of calcium mineralized nodules...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29214143/the-potential-of-micrornas-for-stem-cell-based-therapy-for-degenerative-skeletal-diseases
#20
REVIEW
Emma Budd, Shona Waddell, María C de Andrés, Richard O C Oreffo
Purpose of Review: Degenerative skeletal disorders including osteoarthritis (OA) and osteoporosis (OP) are the result of attenuation of tissue regeneration and lead to painful conditions with limited treatment options. Preventative measures to limit the onset of OA and OP remain a significant unmet clinical need. MicroRNAs (miRNAs) are known to be involved in the differentiation of stem cells, and in combination with stem cell therapy could induce skeletal regeneration and potentially prevent OA and OP onset...
2017: Current Molecular Biology Reports
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