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https://www.readbyqxmd.com/read/28803607/effects-of-scutellaria-baicalensis-on-activity-and-biofilm-formation-of-klebsiella-pneumoniae
#1
Wei Chen, Bei Li, Shuai Li, Yi-Wen Ou, Qin Ou
Objective To explore the effects of Scutellaria baicalensis on activity and biofilm formation of Klebsiella pneumonia (Kp).Methods The broth and agar dilution Methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of Scutellaria baicalensis for TW518. VITEK-32 system was used to assay TW518 susceptibility to antibiotics. Kp biofilms were formed in vitro and stained with BacLight Live/Dead stain. The class integron geneⅠ1 mRNA expression was analyzed with RT-PCR...
September 20, 2016: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/28803185/risk-of-allergic-reactions-to-recurrent-intravenous-penicillin-administration-in-penicillin-skin-test-negative-patients
#2
Steve M Dorman, Sharon Seth, David A Khan
BACKGROUND: Patients with a history of penicillin allergy who are found to be skin test negative to penicillin are able to tolerate repeated oral doses of penicillin with low rates of resensitization. However, the resensitization rate after repeated doses of intravenous penicillin is less clear. OBJECTIVE: We sought to evaluate the risk of allergic reactions to repeated doses of intravenous penicillin in patients who previously reported penicillin allergy and were found to be penicillin skin test and oral challenge negative...
August 9, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28802796/antibacterial-prophylaxis-for-gram-positive-and-gram-negative-infections-in-cranial-surgery-a-meta-analysis
#3
REVIEW
Pranav Abraham, Nayan Lamba, Michael Acosta, Joanna Gholmie, Hassan Y Dawood, Matthew Vestal, Kevin Huang, Maher Hulou, Morteza Asgarzadeh, Hasan Zaidi, Rania A Mekary, Timothy R Smith
BACKGROUND: Perioperative antibiotic prophylaxis against gram positive and gram negative infections is considered standard of care in the perioperative management of patients undergoing cranial surgery. The antibiotic regimen which best reduces the risk of surgical site infections (SSIs) remains controversial. OBJECTIVES: A systematic literature review and meta-analysis were conducted to examine the effect of various prophylactic antibiotics on infection incidence among patients undergoing cranial surgeries...
August 9, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28798146/bloody-stools-in-a-3-day-old-term-infant
#4
Amelia Bray-Aschenbrenner, L Richard Feldenberg, Amelia Kirby, Colleen M Fitzpatrick, Justin B Josephsen
A 3-day-old term, male infant presented to the emergency department for evaluation of bloody stools. The infant was born after an uncomplicated pregnancy followed by a normal spontaneous vaginal delivery. The mother was group B Streptococcus colonized, and received antenatal penicillin prophylaxis. The infant received routine delivery room care, and was given ophthalmic erythromycin and intramuscular vitamin K. Circumcision was performed without bleeding and he was discharged from the newborn nursery and the hospital after 48 hours...
August 10, 2017: Pediatrics
https://www.readbyqxmd.com/read/28797286/prevalence-and-drug-susceptibility-pattern-of-group-b-streptococci-gbs-among-pregnant-women-attending-antenatal-care-anc-in-nekemte-referral-hospital-nrh-nekemte-ethiopia
#5
Hylemariam Mihiretie Mengist, Olifan Zewdie, Adugna Belew, Regea Dabsu
OBJECTIVE: The main objective of this study was to determine the prevalence and drug susceptibility pattern of group B Streptococci (GBS) among pregnant women. The specific objectives include; (1) To determine the prevalence of GBS colonization among pregnant women (2) To determine the drug susceptibility pattern of GBS among pregnant women and (3) To identify associated risk factors with GBS colonization among pregnant women. RESULTS: The median age of the participants was 24...
August 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28796905/aerobic-uterine-isolates-and-antimicrobial-susceptibility-in-mares-with-post-partum-metritis
#6
M S Ferrer, R Palomares
BACKGROUND: The potential for life-threatening complications of metritis requires prompt initiation of antimicrobial treatment, often before microbiological test results are reported. However, published studies to guide first-line antibiotic selection are scarce. OBJECTIVES: To report the most frequent bacterial species, antimicrobial susceptibility and prevalence of multidrug resistance (MDR) in cases of equine metritis. STUDY DESIGN: Retrospective analysis of uterine bacterial culture and antimicrobial susceptibility test (AST) results from mares with metritis...
August 10, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28792086/functional-redundancy-of-division-specific-penicillin-binding-proteins-in-bacillus-subtilis
#7
Jad Sassine, Meizhu Xu, Karzan R Sidiq, Robyn Emmins, Jeff Errington, Richard A Daniel
Bacterial cell division involves the dynamic assembly of a diverse set of proteins that coordinate the invagination of the cell membrane and synthesis of cell wall material to create the new cell poles of the separated daughter cells. Pencillin-binding protein PBP 2B is a key cell division protein in Bacillus subtilis proposed to have a specific catalytic role in septal wall synthesis. Unexpectedly, we find that a catalytically inactive mutant of PBP 2B supports cell division, but in this background the normally dispensable PBP 3 becomes essential...
August 9, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28790348/use-antibiotics-in-cell-culture-with-caution-genome-wide-identification-of-antibiotic-induced-changes-in-gene-expression-and-regulation
#8
Ann H Ryu, Walter L Eckalbar, Anat Kreimer, Nir Yosef, Nadav Ahituv
Standard cell culture guidelines often use media supplemented with antibiotics to prevent cell contamination. However, relatively little is known about the effect of antibiotic use in cell culture on gene expression and the extent to which this treatment could confound results. To comprehensively characterize the effect of antibiotic treatment on gene expression, we performed RNA-seq and ChIP-seq for H3K27ac on HepG2 cells, a human liver cell line commonly used for pharmacokinetic, metabolism and genomic studies, cultured in media supplemented with penicillin-streptomycin (PenStrep) or vehicle control...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28789605/analysis-of-distribution-and-antibiotic-resistance-of-pathogens-isolated-from-the-paediatric-population-in-shenmu-hospital-from-2011-2015
#9
Yang Hui-Min, Wang Yan-Ping, Yong Lin Liu, Bilal Haider Shamsi, He Bo, Meng Xu-Chun
Objective This study aimed to investigate the epidemiology and changes in antibacterial susceptibility of children in Shenmu City, northern Shaanxi, and provide a basis for rational drug use. Methods The distribution and drug resistance pattern of pathogenic bacteria isolated from children were retrospectively analysed. Results A total of 573 strains of pathogens were cultivated. A total of 201 (35.07%) strains of Gram-positive cocci and 183 (31.93%) strains of Gram-negative cocci were detected. A total of 189 (32...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28783775/low-grade-fever-erythematous-rash-in-pregnant-woman-%C3%A2-dx
#10
James M O'Brien, Jonathan D Baum
A 31-year-old woman presented to her obstetrician's office at 16 weeks' gestation with a 2-day history of low-grade fever and an erythematous rash measuring 1 x 4 cm on her right groin. She had a medical history of a penicillin allergy (urticarial) and her outdoor activities included gardening and picnicking. What's your diagnosis?
August 2017: Journal of Family Practice
https://www.readbyqxmd.com/read/28783024/pneumococcal-nasopharyngeal-carriage-in-young-healthy-children-after-pneumococcal-conjugate-vaccine-in-turkey
#11
Ahmet Arvas, Haluk Çokuğraş, Emel Gür, Nevriye Gönüllü, Zeynep Taner, Hrisi Bahar Tokman
AIMS: To determine the prevalence of nasopharyngeal carriage of Streptococcus pneumoniae in healthy children aged 0-6 years who were vaccinated with pneumococcal conjugate vaccine. METHODS: This cross-sectional study was conducted on 150 healthy Turkish children between 1 month and 6 years of age. Serotyping was performed and risk factors of carriage were evaluated. RESULTS: The overall carriage rate was 14%. Vaccine type serotypes were determined in 17 (12...
August 4, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28779997/the-bacterial-species-associated-with-aspirated-foreign-bodies-in-children
#12
Maayan Gruber, Graeme van Der Meer, Benjamin Ling, Colin Barber, Nikki Mills, Michel Neeff, Lesley Salkeld, Murali Mahadevan
OBJECTIVE: Inhaled foreign bodies in children are common and may be complicated by secondary airway tract infection. The inhaled foreign body may act as carrier of infectious material and the aim of this study was to explore the bacterial species associated with aspirated foreign bodies in a cohort of children. METHODS: Retrospective case series of 34 patients who underwent rigid laryngobronchoscopy because of foreign body aspiration. Each patient had a sample taken from tracheobronchial secretions during the procedure...
August 2, 2017: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/28776337/changes-in-the-serotype-distribution-among-antibiotic-resistant-carriage-streptococcus-pneumoniae-isolates-in-children-after-the-introduction-of-the-extended-valency-pneumococcal-conjugate-vaccine
#13
Joon Kee Lee, Ki Wook Yun, Eun Hwa Choi, Sun Jung Kim, Seong Yeon Lee, Hoan Jong Lee
This study investigated the serotype distribution and antimicrobial resistance of 3,820 nasopharyngeal Streptococcus pneumoniae isolates from infants and children who presented with respiratory symptoms at Seoul National University Children's Hospital from July 2010 to June 2015 after the introduction of the extended-valency pneumococcal conjugate vaccines (PCVs). Serotypes and antimicrobial susceptibility were determined using the Quellung reaction and E-test, respectively. S. pneumoniae was isolated from 397 (10...
September 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28776170/viridans-group-streptococci-bloodstream-infections-in-neutropenic-adult-patients-with-hematologic-malignancy-single-center-experience
#14
J Radocha, P Paterová, A Zavřelová, B Víšek, F Gabalec, H Žemličková, P Žák
Viridans group streptococci bloodstream infections (VGS BSI) remain a significant cause of mortality and morbidity in patients with severe neutropenia. The goal of our study was to evaluate clinical course and microbiological susceptibility of VGS BSI at our center. Retrospective analysis of all microbiologically documented bloodstream infections caused by VGS during the 9-year time period (from January 2006 until December 2014) was carried out. Only patients with severe neutropenia (< 500/μL) were included in the study...
August 3, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28774639/draft-genome-sequence-of-chryseobacterium-limigenitum-sur2-t-lmg-28734-t-isolated-from-dehydrated-sludge
#15
Jure Škraban, Nikos C Kyrpides, Nicole Shapiro, William B Whitman, Janja Trček
The type strain SUR2 of the novel species Chryseobacterium limigenitum was isolated from a dehydrated sludge of the municipal sewage treatment plant in Dogoše near Maribor in Slovenia. The draft genome, with 60 contigs, 4,697,725bp, 34.4% of G+C content, was obtained using the Illumina HiSeq 2500-1 platform. Joint Genome Institute Microbial Genome Annotation Pipeline (MGAP v.4) has identified 4322 protein-coding sequences including resistance genes against arsenic and other heavy metals. In addition, a subclass B3 metallo-β-lactamase, which confers resistance to penicillins, cephalosporins and carbapenems, was also present in the genome...
July 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28771537/a-spirochaete-is-suggested-as-the-causative-agent-of-akoya-oyster-disease-by-metagenomic-analysis
#16
Tomomasa Matsuyama, Motoshige Yasuike, Atushi Fujiwara, Yoji Nakamura, Tomokazu Takano, Takeshi Takeuchi, Noriyuki Satoh, Yoshikazu Adachi, Yasushi Tsuchihashi, Hideo Aoki, Kazushi Odawara, Shunsuke Iwanaga, Jun Kurita, Takashi Kamaishi, Chihaya Nakayasu
Mass mortality that is acompanied by reddish browning of the soft tissues has been occurring in cultured pearl oyster, Pinctada fucata martensii. The disease is called Akoya oyster disease (AOD). Although spreading pattern of the disease and transmission experiments suggest that the disease is infectious, the causative agent has not yet been identified. We used shotgun and 16S rRNA-based metagenomic analysis to identify genes that are present specifically in affected oysters. The genes found only in diseased oysters were mostly bacterial origin, suggesting that the causative agent was a bacterial pathogen...
2017: PloS One
https://www.readbyqxmd.com/read/28771160/staphylococcus-aureus-isolated-from-wastewater-treatment-plants-in-tunisia-occurrence-of-human-and-animal-associated-lineages
#17
Meriam Ben Said, Mohamed Salah Abbassi, Paula Gómez, Laura Ruiz-Ripa, Senda Sghaier, Chourouk Ibrahim, Carmen Torres, Abdennaceur Hassen
The objective was to characterize Staphylococcus aureus isolated from two wastewater treatment plants (WWTPs) located in Tunis City (Tunisia), during the period 2014-2015. Genetic lineages, antibiotic resistance mechanisms and virulence factors were determined for the recovered isolates. S. aureus isolates were recovered from 12 of the 62 wastewater samples tested (19.35%), and one isolate/sample was characterized, all of them being methicillin-susceptible (MSSA). Six spa types (t587, t674, t224, t127, t701 and t1534) were found among the 12 isolates, and the spa-t587, associated with the new sequence type ST3245, was the most predominant one (7 isolates)...
August 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/28769912/xanthomonas-citri-minc-oscillates-from-pole-to-pole-to-ensure-proper-cell-division-and-shape
#18
André S G Lorenzoni, Giordanni C Dantas, Tessa Bergsma, Henrique Ferreira, Dirk-Jan Scheffers
Xanthomonas citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. To further characterize cell division in this plant pathogen, we investigated the role of the protein MinC in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minC using allele exchange. Xac with minC deleted exhibited the classic Δmin phenotype observed in other bacteria deleted for min components: minicells and short filamentation...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28768452/aetiology-and-clinical-features-of-facial-cellulitis-a-prospective-study
#19
Eivind Rath, Steinar Skrede, Haima Mylvaganam, Trond Bruun
BACKGROUND: In the early 20th century, the face was the predominant site of cellulitis. Despite a relative decrease in the incidence of facial cellulitis, it is still common. There are few studies on this condition during the last decades. The aim of this study was to describe contemporary aetiological and clinical characteristics of patients admitted to hospital with non-suppurative facial cellulitis. METHODS: Patients were included prospectively. Clinical details, comorbidities and biochemistry results were recorded...
August 2, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28766108/a-pharmacoepidemiology-database-system-for-monitoring-risk-due-to-the-use-of-medicines-by-new-zealand-primary-care-patients
#20
Andrew M Tomlin, David M Reith, David J Woods, Hywel S Lloyd, Alesha Smith, John S Fountain, Murray W Tilyard
INTRODUCTION: The use of large record-linked healthcare databases for drug safety research and surveillance is now accepted practice. New Zealand's standardized national healthcare datasets provide the potential to automate the conduct of pharmacoepidemiological studies to provide rapid validation of medicine safety signals. OBJECTIVES: Our objectives were to describe the methodology undertaken by a semi-automated computer system developed to rapidly assess risk due to drug exposure in New Zealand's population of primary care patients and to compare results from three studies with previously published findings...
August 1, 2017: Drug Safety: An International Journal of Medical Toxicology and Drug Experience
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