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https://www.readbyqxmd.com/read/29321644/cox-2-induces-oncogenic-micro-rna-mir655-in-human-breast-cancer
#1
Mousumi Majumder, Leanna Dunn, Ling Liu, Asma Hasan, Krista Vincent, Muriel Brackstone, David Hess, Peeyush K Lala
We show that Cyclooxygenase-2 over-expression induces an oncogenic microRNA miR655 in human breast cancer cells by activation of EP4. MiR655 expression positively correlated with COX-2 in genetically disparate breast cancer cell lines and increased in all cell lines when grown as spheroids, implicating its link with stem-like cells (SLCs). Ectopic miR655 over-expression in MCF7 and SKBR3 cells resulted in increased proliferation, migration, invasion, spheroid formation and Epithelial to Masenchymal transition (EMT)...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29281830/oncogenic-myc-activates-a-feedforward-regulatory-loop-promoting-essential-amino-acid-metabolism-and-tumorigenesis
#2
Ming Yue, Jue Jiang, Peng Gao, Hudan Liu, Guoliang Qing
Most tumor cells exhibit obligatory demands for essential amino acids (EAAs), but the regulatory mechanisms whereby tumor cells take up EAAs and EAAs promote malignant transformation remain to be determined. Here, we show that oncogenic MYC, solute carrier family (SLC) 7 member 5 (SLC7A5), and SLC43A1 constitute a feedforward activation loop to promote EAA transport and tumorigenesis. MYC selectively activates Slc7a5 and Slc43a1 transcription through direct binding to specific E box elements within both genes, enabling effective EAA import...
December 26, 2017: Cell Reports
https://www.readbyqxmd.com/read/29215309/can-teacher-child-relationships-support-human-rights-to-freedom-of-opinion-and-expression-education-and-participation
#3
Cen Wang, Linda J Harrison, Sharynne McLeod, Sue Walker, Jantine L Spilt
PURPOSE: This study explored how teacher-child relationships change over the early school years, in terms of closeness and conflict, whether these trajectories differ in type and frequency for children with typical development and children with speech and language concern (SLC), and whether the trajectories are associated with school outcomes at 12-13 years. METHOD: Participants were children, parents and teachers in the Longitudinal Study of Australian Children...
December 7, 2017: International Journal of Speech-language Pathology
https://www.readbyqxmd.com/read/29212823/quantitative-expression-of-hepatobiliary-transporters-and-functional-uptake-of-substrates-in-hepatic-2d-sandwich-cultures-a-comparative-evaluation-of-upcyte-and-primary-human-hepatocytes
#4
Michelle Schaefer, Gaku Morinaga, Akiko Matsui, Gerhard Schanzle, Daniel Bischoff, Roderich D Sussmuth
Deficient functional expression of drug transporters incapacitates most hepatic cell lines as reliable tool for evaluating transporter-mediated drug-drug interactions. Recently, genetically-modified cells, referred to as upcyte hepatocytes, have emerged as an expandable, non‑cancerous source of human hepatic cells. Herein, we quantified mRNA and protein levels of key hepatobiliary transporters and assessed associated uptake activity in short- and long-term cultures of upcyte human hepatocytes (UHH), in comparison to cryopreserved primary human hepatocytes (cPHH)...
December 6, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29204754/the-na-cl-coupled-broad-specific-amino-acid-transporter-slc6a14-atb0-emerging-roles-in-multiple-diseases-and-therapeutic-potential-for-treatment-and-diagnosis
#5
Mohd Omar F Sikder, Shengping Yang, Vadivel Ganapathy, Yangzom D Bhutia
Amino acids are essential building blocks of all mammalian cells, and amino acid transporters play a vital role in transporting them into cells and their further distribution among the various cellular compartments. There are ~ 430 known transporters in the solute-linked carrier (SLC) gene family, divided into 52 distinct families. Eleven of these gene families contain one or more amino acid transporters. These transporters differ significantly from each other in terms of substrate specificity, ion dependence, and energetics...
December 4, 2017: AAPS Journal
https://www.readbyqxmd.com/read/29191653/distorted-antibody-repertoire-developed-in-the-absence-of-pre-bcr-formation
#6
Lin Sun, Naoko Kono, Takeyuki Shimizu, Hiroyuki Toh, Hanbing Xue, Osamu Numata, Manabu Ato, Shigeyuki Itamura, Kazuo Ohnishi
The pre-B cell receptor (pre-BCR), consisting of the μ heavy chain (μHC) and the surrogate light chain (SLC, Vpre-B, and λ5), plays important roles during B cell development. The formation of the pre-BCR, which enables the nascent immunoglobulin HC to associate with the SLC, is considered a prerequisite for B cell development. However, a significant number of peripheral mature (leaky) B cells exist in SLC-deficient mice. These leaky B cells develop in the absence of pre-BCR and do not undergo the pre-BCR checkpoint...
November 27, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29163820/role-of-drug-dependent-transporter-modulation-on-the-chemosensitivity-of-cholangiocarcinoma
#7
Nerea Urtasun, Clara Boces-Pascual, Loreto Boix, Jordi Bruix, Marçal Pastor-Anglada, Sandra Pérez-Torras
Cholangiocarcinoma (CCA) is a heterogeneous group of malignancies with limited therapeutic options. Curative therapy is limited to surgery whereas chemotherapy treatments are the election option for unresectable or metastatic cholangiocarcinoma. Cisplatin plus gemcitabine is the reference chemotherapy regimen, albeit the contribution to the median overall survival barely reaches one year. Drug transporters are undoubtedly a limiting step for drug bioavailability and have been clearly related to chemoresistance...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29151946/gene-expression-profiling-reveals-novel-candidate-markers-of-ovarian-carcinoma-intraperitoneal-metastasis
#8
Katerina Elsnerova, Alena Bartakova, Josef Tihlarik, Jiri Bouda, Lukas Rob, Petr Skapa, Martin Hruda, Ivan Gut, Beatrice Mohelnikova-Duchonova, Pavel Soucek, Radka Vaclavikova
Epithelial ovarian cancer (EOC) has the highest mortality among gynecological carcinomas. The lack of specific markers for prognostic determination of EOC progression hinders the search for novel effective therapies. The aim of the present study was (i) to explore differences in expressions of ATP-binding cassette (ABC) and solute carrier (SLC) transporter genes, genes associated with drug metabolism and cell cycle regulation between control ovarian tissues (n = 14), primary EOCs (n = 44) and intraperitoneal metastases (n = 29); (ii) to investigate associations of gene expression levels with prognosis of patients with intraperitoneal metastases...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29123324/three-decades-of-landsat-derived-spring-surface-water-dynamics-in-an-agricultural-wetland-mosaic-implications-for-migratory-shorebirds
#9
Danica Schaffer-Smith, Jennifer J Swenson, Blake Barbaree, Matthew E Reiter
Satellite measurements of surface water offer promise for understanding wetland habitat availability at broad spatial and temporal scales; reliable habitat is crucial for the persistence of migratory shorebirds that depend on wetland networks. We analyzed water extent dynamics within wetland habitats at a globally important shorebird stopover site for a 1983-2015 Landsat time series, and evaluated the effect of climate on water extent. A range of methods can detect open water from imagery, including supervised classification approaches and thresholds for spectral bands and indices...
May 2017: Remote Sensing of Environment
https://www.readbyqxmd.com/read/29115014/impact-of-variant-microscopic-interpretation-of-the-ucyt-immunocytological-urine-test-for-the-detection-of-bladder-cancer
#10
Susanne Deininger, Tilman Todenhöfer, Jörg Hennenlotter, Valentina Gerber, Jennifer Schwarz, Jens Bedke, Christian Schwentner, Arnulf Stenzl, Steffen Rausch
BACKGROUND: Urinary marker tests for bladder cancer (BC) detection and surveillance represent a desirable approach to diagnosis and follow-up. The SCIMEDX uCyt+ assay detects antigens expressed by BC cells (mucin glycoprotein and carcinoembryonic antigen) using green and red fluorescence and is interpreted according to specific manufacturer's recommendations. In the present study, we evaluated divergent approaches of numeric and morphological analysis of uCyt+ to generate a rationale for alternative test interpretation strategies...
November 8, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29113727/effect-of-single-layer-centrifugation-porcicoll-70-80-and-90-or-supplementation-with-reduced-glutathione-seminal-plasma-and-bovine-serum-albumin-on-frozen-thawed-boar-sperm
#11
I Crespo-Félez, A Castañeda-Sampedro, D I Sánchez, E Fernández-Alegre, M Álvarez-Rodríguez, J C Domínguez, J M Morrell, F Martínez-Pastor
Selecting the optimal sperm population is essential for success with reproductive techniques. Porcicoll (formerly Androcoll-P) is a colloid formulation for selection of high-quality boar spermatozoa by single layer centrifugation (SLC). To date, most studies have been carried out with fresh semen and large volumes. We carried out 2 experiments to test the use of Porcicoll for thawed boar semen in small volumes. In Experiment 1, cryopreserved semen doses were thawed, split in 200-μL aliquots and layered on 1mL of Porcicoll 70%, 80% or 90%, or buffer without colloid...
November 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29109021/renal-organic-anion-transporters-in-drug-drug-interactions-and-diseases
#12
REVIEW
Xiaokui Huo, Kexin Liu
The kidney plays a vital role in maintaining systemic homeostasis. Active tubular secretion and reabsorption, which are mainly mediated by transporters, is an efficient mechanism for retaining glucose, amino acids, and other nutrients and for the clearance of endogenous waste products and xenobiotics. These substances are recognized by uptake transporters located in the basolateral and apical membranes of renal proximal tubule cells and are extracted from plasma and urine. Organic anion transporters (OATs) belong to the solute carrier (SLC) 22 superfamily and facilitate organic anions across the plasma membranes of renal proximal tubule cells...
November 8, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29084097/differential-effects-of-different-warm-up-protocols-on-repeated-sprints-induced-muscle-damage
#13
Che-Hsiu Chen, Xin Ye, Yi-Tse Wang, Yung-Sheng Chen, Wei-Chin Tseng
The purpose of this investigation was to examine whether adding a set of hamstring resistance exercise or dynamic stretching to a regular running-based warm-up before a bout of repeated sprints provides protective effects against the sprinting-induced muscle damage. Twelve elite tennis players participated in this study. After the familiarization, subjects completed 3 separate randomly sequenced experimental visits, during which 3 different warm-up interventions were performed before the muscle-damaging protocol (12 sets of 30-m maximal repeated sprints): 5 minutes of running (control); control with single leg slide curl (SLC); and control with active hamstring stretching (AHS)...
October 27, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29049335/structural-prediction-of-two-novel-human-atypical-slc-transporters-mfsd4a-and-mfsd9-and-their-neuroanatomical-distribution-in-mice
#14
Emelie Perland, Sofie Victoria Hellsten, Nadine Schweizer, Vasiliki Arapi, Fatemah Rezayee, Mona Bushra, Robert Fredriksson
Out of the 430 known solute carriers (SLC) in humans, 30% are still orphan transporters regarding structure, distribution or function. Approximately one third of all SLCs belong to the evolutionary conserved and functionally diverse Major Facilitator Superfamily (MFS). Here, we studied the orphan proteins, MFSD4A and MFSD9, which are atypical SLCs of MFS type. Hidden Markov Models were used to identify orthologues in several vertebrates, and human MFSD4A and MFSD9 share high sequence identity with their identified orthologues...
2017: PloS One
https://www.readbyqxmd.com/read/29048856/-rapid-determination-of-four-phenolic-environmental-estrogen-residues-in-cooking-oil-by-ultra-performance-liquid-chromatography-high-resolution-mass-spectrometry-coupled-with-solid-phase-extraction
#15
Shengdong Pan, Qian He, Xiaohong Chen, Li Wang, Qiaoli Qiu, Micong Jin
A fast, sensitive and accurate method for the determination of trace bisphenol S (BPS), bisphenol F (BPF), bisphenol A (BPA) and 4-nonylphenol (4-NP) in cooking oil samples was developed by ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) coupled with solid-phase extraction (SPE). Cooking oil samples were extracted by acetonitrile, then the supernatant was purified by SLC SPE cartridges. The chromatographic separation was carried out on a Waters ACQUITY UPLC HSS T3 column (100 mm×2...
September 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29032766/the-effects-of-drug-metabolizing-enzyme-inhibitors-on-hepatic-efflux-and-uptake-transporters
#16
Jonathan Cheong, Jason S Halladay, Jasleen K Sodhi, Emile Plise, Laurent Salphati
BACKGROUND: Non-selective chemical inhibitors of phase I and phase II enzymes are commonly used in in vitro metabolic studies to elucidate the biotransformation pathways of drugs. However, the inhibition of the inhibitors on efflux and uptake transporters is not well investigated, potentially leading to unexpected and ambiguous results in these studies. OBJECTIVE: The commonly used metabolizing enzyme inhibitors, 1-aminobenzotriazole (ABT), SKF-525A, pargyline, allopurinol, menadione, methimazole, piperine and raloxifene, were examined for their potential inhibition of the major hepatic ABC (ATP binding cassette) and SLC (solute carrier) transporters...
October 10, 2017: Drug Metabolism Letters
https://www.readbyqxmd.com/read/29031804/transcriptional-response-after-exposure-to-domoic-acid-producing-pseudo-nitzschia-in-the-digestive-gland-of-the-mussel-mytilus-galloprovincialis
#17
Antonio J Pazos, Pablo Ventoso, Roi Martínez-Escauriaza, M Luz Pérez-Parallé, Juan Blanco, Juan C Triviño, José L Sánchez
Bivalve molluscs are filter feeding species that can accumulate biotoxins in their body tissues during harmful algal blooms. Amnesic Shellfish Poisoning (ASP) is caused by species of the diatom genus Pseudo-nitzschia, which produces the toxin domoic acid. The Mytilus galloprovincialis digestive gland transcriptome was de novo assembled based on the sequencing of 12 cDNA libraries, six obtained from control mussels and six from mussels naturally exposed to domoic acid-producing diatom Pseudo-nitzschia australis...
October 12, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29022899/transplanted-human-p75-positive-stem-leydig-cells-replace-disrupted-leydig-cells-for-testosterone-production
#18
Min Zhang, Jiancheng Wang, Chunhua Deng, Mei Hua Jiang, Xin Feng, Kai Xia, Weiqiang Li, Xingqiang Lai, Haipeng Xiao, Ren-Shan Ge, Yong Gao, Andy Peng Xiang
Previous studies have demonstrated that rodent stem Leydig cell (SLC) transplantation can partially restore testosterone production in Leydig cell (LC)-disrupted or senescent animal models, which provides a promising approach for the treatment of hypogonadism. Here, we isolated human SLCs prospectively and explored the potential therapeutic benefits of human SLC transplantation for hypogonadism treatment. In adult human testes, p75 neurotrophin receptor positive (p75(+)) cells expressed the known SLC marker nestin, but not the LC lineage marker hydroxysteroid dehydrogenase-3β (HSD3β)...
October 12, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28989017/mechanistic-basis-of-cocrystal-dissolution-advantage
#19
Fengjuan Cao, Gordon L Amidon, Naír Rodríguez-Hornedo, Gregory E Amidon
Current interest in cocrystal development resides in the advantages that the cocrystal may have in solubility and dissolution compared to the parent drug. This work provides a mechanistic analysis and comparison of the dissolution behavior of carbamazepine (CBZ) and its two cocrystals, carbamazepine-saccharin (CBZ-SAC) and carbamazepine-salicylic acid (CBZ-SLC) under the influence of pH and micellar solubilization. A simple mathematical equation is derived based on the mass transport analyses to describe the dissolution advantage of cocrystals...
October 5, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28984791/sex-determining-region-y-box-9-induces-chemoresistance-in-pancreatic-cancer-cells-by-induction-of-putative-cancer-stem-cell-characteristics-and-its-high-expression-predicts-poor-prognosis
#20
Taku Higashihara, Hideyuki Yoshitomi, Yasuyuki Nakata, Shingo Kagawa, Shigetsugu Takano, Hiroaki Shimizu, Atsushi Kato, Katsunori Furukawa, Masayuki Ohtsuka, Masaru Miyazaki
OBJECTIVES: Pancreatic cancer is a highly chemoresistant tumor and underlying mechanisms are not well understood. Sex determining region Y box 9 (Sox9) is a transcription factor playing important roles on maintenance of pluripotent cells during pancreatic organogenesis. The purpose of this study is to evaluate the roles of Sox9 in pancreatic cancer. METHODS: The Sox9 expression was evaluated by immunohistochemical analysis. Effects of Sox9 inhibition by siRNA or shRNA on chemosensitivity, sphere formation, stem cell markers expression, and in vivo tumor formation rate were examined using pancreatic cancer cell lines...
November 2017: Pancreas
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