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https://www.readbyqxmd.com/read/28330122/production-purification-and-characterization-of-a-thermotolerant-alkaline-serine-protease-from-a-novel-species-bacillus-caseinilyticus
#1
Thirumala Mothe, Vishnuvardhan Reddy Sultanpuram
Alkaline proteases are important enzymes in many industrial applications, especially as additives in laundry detergent industry. Though there are a number of Bacillus species which are reported to be producing proteases, the efficiency of a protease produced by a novel strain has to be studied in comparison to the others. Hence, in this study, an alkaline serine protease produced by a novel species Bacillus caseinilyticus was purified and characterized for its possible usage in detergent industry. Ammonium sulphate, dialysis and DEAE column chromatographic methods were used for purification of the isolated alkaline protease...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28315219/assessment-of-ige-reactivity-of-%C3%AE-casein-by-western-blotting-after-digestion-with-simulated-gastric-fluid
#2
Sara Benedé, Rosina López-Fandiño, Elena Molina
Cow's milk allergy is defined as an immunologically mediated adverse reaction to cow's milk proteins and it is usually, along with hen's egg allergy, the first food allergy identified in childhood.One of the main aspects to consider when evaluating the allergenic potential of food proteins is the effect of gastric digestion. It is known that allergens are usually able to survive the harsh acidic environment of the stomach, tolerate the presence of surfactants, and resist digestion by pepsin. They might also be digested into high molecular weight peptide fragments, which retain the same, or sometimes increased, IgE-binding...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28291369/ultrashort-single-walled-carbon-nanotubes-insert-into-pulmonary-surfactant-monolayer-via-self-rotating-poration-and-mechanical-inhibition
#3
Tongtao Yue, Yan Xu, Shixin Li, Zhen Luo, Xianren Zhang, Fang Huang
It has been widely accepted that longer single-walled carbon nanotubes (SWCNTs) exhibit higher toxicity by causing severe pneumonia once inhaled, yet relatively little is known regarding the potential toxicity of ultrashort SWCNTs, which are of central importance to their development as suitable vehicles for biomedical applications. Here, by combining coarse-grained molecular dynamics (CGMD), pulling simulations, and scaling analysis, we demonstrate that the inhalation toxicity of ultrashort SWCNTs (1.5 nm < l < 5...
March 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28288148/natriuretic-peptide-c-receptor-in-the-developing-sheep-lung-role-in-perinatal-transition
#4
Bobby Mathew, Christopher A D'Angelis, Satyan Lakshminrusimha, Peter A Nickerson, June J Sokolowski, Vasantha H S Kumar, Huamei Wang, Karen A Wynn, Bruce A Holm, Rita M Ryan
BACKGROUND: At birth, the release of surfactant from Alveolar Type II (ATII) cells is stimulated by increased activity of the beta-adrenergic/adenylyl cyclase/cAMP- signaling cascade. Atrial natriuretic peptide (ANP) stimulates surfactant secretion through Natriuretic peptide receptor A (NPR-A). ANP inhibits adenylyl cyclase activity through its binding to NPR-C. We wished to further understand the role of the NPR-C in perinatal transition. METHODS: We studied fetal and newborn sheep ATII expression of NPR-C and surfactant secretion in isolated ATIIs by measuring (3)[H] choline release into the media...
March 13, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28282112/peptide-cross-linkers-immobilization-of-platinum-nanoparticles-highly-dispersed-on-graphene-oxide-nanosheets-with-enhanced-photocatalytic-activities
#5
Tsukasa Mizutaru, Galina Marzun, Sebastian Kohsakowski, Stephan Barcikowski, Dachao Hong, Hiroaki Kotani, Takahiko Kojima, Takahiro Kondo, Junji Nakamura, Yohei Yamamoto
For exerting potential catalytic and photocatalytic activities of metal nanoparticles (MNPs), immobilization of MNPs on a support medium in highly dispersed state is desired. In this Research Article, we demonstrated that surfactant-free platinum nanoparticles (PtNPs) were efficiently immobilized on graphene oxide (GO) nanosheets in a highly dispersed state by utilizing oligopeptide β-sheets as a cross-linker. The fluorenyl-substituted peptides were designed to form β-sheets, where metal-binding thiol groups and protonated and positively charged amino groups are integrated on the opposite sides of the surface of a β-sheet, which efficiently bridge PtNPs and GO nanosheet...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28277006/cationic-copolymer-augments-membrane-permeabilizing-activity-of-an-amphiphilic-peptide
#6
Wakako Sakamoto, Takuro Ochiai, Naohiko Shimada, Atsushi Maruyama
Membrane disruptive peptides (also called membrane fusogenic peptides) have been employed for cytosolic delivery of macromolecules such as nucleic acids and proteins. We reported previously that the cationic graft copolymer, poly(allylamine)-graft-dextran (PAA-g-Dex), augments membrane disruptive activity of the negatively charged E5 peptide. Strong membrane disruptive activity was observed in the presence of the copolymer at both acidic and neutral pH. In this paper, activities of E5/PAA-g-Dex mixture were further explored...
February 17, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28271630/sample-clean-up-strategies-for-electrospray-ionization-mass-spectrometry-applications-in-bottom-up-proteomics-trends-from-2012-2016
#7
REVIEW
Ria Marni Tubaon, Paul R Haddad, Joselito P Quirino
Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favoured over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sample or produced during tryptic digestion are detrimental in MS analysis and affect the proteome data, thus sample preparation for removal of these unwanted components has become essential. Here, we review 48 research papers on strategies for removal of salts and surfactants (in particular, sodium dodecyl sulfate, SDS) prior to electrospray ionization (ESI)-MS analysis in bottom-up proteomics from 2012-2016...
March 8, 2017: Proteomics
https://www.readbyqxmd.com/read/28254280/effects-of-alkyl-polyglycoside-apg-on-bombyx-mori-silk-degumming-and-the-mechanical-properties-of-silk-fibroin-fibre
#8
Fei Wang, Yu-Qing Zhang
Alkyl polyglycoside (APG), a nonionic surfactant, is often considered to be a green surfactant and is synthesized using glucose and long chain fatty alcohols. It is used as a degumming agent of Bombyx mori silk fibre in this study for the first time. We studied APG systematically in comparison to the traditional degumming methods, such as aqueous solutions of sodium carbonate (Na2CO3) and neutral soap (NS). After repeatedly boiling silk fibres in an aqueous solution of 0.25% APG three times for 30min and using a bath ratio of 1:90-120 (g/mL), sericin was completely removed from the fibre...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28235590/rational-design-of-polyarginine-nanocapsules-intended-to-help-peptides-overcoming-intestinal-barriers
#9
Zhigao Niu, Erik Tedesco, Federico Benetti, Aloïse Mabondzo, Isabella Monia Montagner, Ilaria Marigo, David Gonzalez-Touceda, Sulay Tovar, Carlos Diéguez, Manuel J Santander-Ortega, María J Alonso
The aim of this work was to rationally design and characterize nanocapsules (NCs) composed of an oily core and a polyarginine (PARG) shell, intended for oral peptide delivery. The cationic polyaminoacid, PARG, and the oily core components were selected based on their penetration enhancing properties. Insulin was adopted as a model peptide to assess the performance of the NCs. After screening numerous formulation variables, including different oils and surfactants, we defined a composition consisting of oleic acid, sodium deoxycholate (SDC) and Span 80...
February 21, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28221717/cerebral-and-lung-effects-of-a-new-generation-synthetic-surfactant-with-sp-b-and-sp-c-analogs-in-preterm-lambs
#10
Carmen Rey-Santano, Victoria E Mielgo, Xabier Murgia, Miguel Angel Gomez-Solaetxe, Fabrizio Salomone, Federico Bianco, Nicola Pelizzi, Begoña Loureiro, Jon López de Heredia Y Goya
BACKGROUND: Though natural surfactants (SF) are clinically superior to protein-free synthetic preparations, CHF-5633, a synthetic SF containing SP-B and SP-C analog peptides is a potential alternative to natural SF for treating neonatal respiratory distress syndrome (RDS). Nevertheless, information is lacking regarding the safety of this new treatment for the neonatal brain. We sought to compare the cerebral and pulmonary effects of this new synthetic surfactant (CHF5633) with those of natural porcine surfactant (Cursosurf) in premature lambs with RDS...
February 21, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28191927/foldectures-3d-molecular-architectures-from-self-assembly-of-peptide-foldamers
#11
Sung Hyun Yoo, Hee-Seung Lee
The wide range of fascinating supramolecular architectures found in nature, from DNA double helices to giant protein shells, inspires researchers to mimic the diverse shapes and functions of natural systems. Thus, a variety of artificial molecular platforms have been developed by assembling DNA-, peptide-, and protein-based building blocks for medicinal and biological applications. There has also been a significant interest in the research of non-natural oligomers (i.e., foldamers) that fold into well-defined secondary structures analogous to those found in proteins, because the assemblies of foldamers are expected not only to form biomimetic supramolecular architectures that resemble those of nature but also to display unique functions and unprecedented topologies at the same time due to their different folding propensities from those of natural building blocks...
February 13, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28188875/hydrophobic-ion-pairing-key-to-highly-payloaded-self-emulsifying-peptide-drug-delivery-systems
#12
Janine Griesser, Gergely Hetényi, Michael Moser, Frédéric Demarne, Vincent Jannin, Andreas Bernkop-Schnürch
AIM: The aim of this study was the formation and characterization of various ion pairs of therapeutic peptides with different surfactants in order to reach a high payload in self-emulsifying drug delivering systems (SEDDS). METHODS: Hydrophobic ion pairs (HIP) were formed between the anionic surfactants sodium docusate, dodecylsulfate and oleate and the peptides leuprorelin (LEU), insulin (INS) and desmopressin (DES). The efficiency of HIP formation was evaluated by quantifying the amount of formed complexes, log P value determination in n-octanol/water via HPLC and zeta potential measurements...
February 7, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28188794/surfactant-protein-a-sp-a-and-sp-a-derived-peptide-attenuate-chemotaxis-of-mast-cells-induced-by-human-%C3%AE-defensin-3
#13
Yasuaki Uehara, Motoko Takahashi, Masaki Murata, Atsushi Saito, Rina Takamiya, Yoshihiro Hasegawa, Koji Kuronuma, Hirofumi Chiba, Jiro Hashimoto, Norimasa Sawada, Hiroki Takahashi, Yoshio Kuroki, Shigeru Ariki
Human β-defensin 3 (hBD3) is known to be involved in mast cell activation. However, molecular mechanisms underlying the regulation of hBD3-induced mast cell activation have been poorly understood. We previously reported that SP-A and SP-A-derived peptide 01 (SAP01) regulate the function of hBD3. In this study, we focused on the effects of SP-A and SAP01 on the activation of mast cells induced by hBD3. SAP01 directly bound to hBD3. Mast cell-mediated vascular permeability and edema in hBD3 administered rat ears were decreased when injected with SP-A or SAP01...
March 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28186408/functional-microgels-and-microgel-systems
#14
Felix A Plamper, Walter Richtering
Microgels are macromolecular networks swollen by the solvent in which they are dissolved. They are unique systems that are distinctly different from common colloids, such as, e.g., rigid nanoparticles, flexible macromolecules, micelles, or vesicles. The size of the microgel networks is in the range of several micrometers down to nanometers (then sometimes called "nanogels"). In a collapsed state, they might resemble hard colloids but they can still contain significant amounts of solvent. When swollen, they are soft and have a fuzzy surface with dangling chains...
February 10, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28144135/liposomes-derivatized-with-multimeric-copies-of-kccysl-peptide-as-targeting-agents-for-her-2-overexpressing-tumor-cells
#15
Paola Ringhieri, Silvia Mannucci, Giamaica Conti, Elena Nicolato, Giulio Fracasso, Pasquina Marzola, Giancarlo Morelli, Antonella Accardo
Mixed liposomes, obtained by coaggregation of 1,2-dioleoyl-sn-glycero-3-phosphocholine and of the synthetic monomer containing a gadolinium complex ([C18]2DTPA[Gd]) have been prepared. Liposomes externally decorated with KCCYSL (P6.1 peptide) sequence in its monomeric, dimeric, and tetrameric forms are studied as target-selective delivery systems toward cancer cells overexpressing human epidermal growth factor receptor-2 (HER-2) receptors. Derivatization of liposomal surface with targeting peptides is achieved using the postmodification method: the alkyne-peptide derivative Pra-KCCYSL reacts, through click chemistry procedures, with a synthetic surfactant modified with 1, 2, or 4 azido moieties previously inserted in liposome formulation...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28137696/storage-stability-of-bivalirudin-hydrophilic-vs-lipophilic-solutions
#16
Ožbej Zupančič, Julia Anita Grießinger, Hung Thanh Lam, Andreas Bernkop-Schnürch
It was the aim of this study to incorporate a model peptide bivalirudin in self-emulsifying drug delivery system (SEDDS) and compare its storage stability with conventional aqueous solutions. Firstly, bivalirudin lipophilicity was increased via hydrophobic ion pairing using anionic or cationic surfactants. The chosen bivalirudin docusate complex (BIV/AOT) was incorporated into SEDDS composed of 40% (w/w) Cremophor EL, 20% (w/w) Capmul PG-8 and 40% (w/w) propylene glycol with a drug payload of 0.20% (w/w). SEDDS were stable over a wide pH range for at least 7 days at 37 (0)C and showed an immediate bivalirudin release profile...
January 27, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28125290/molecular-characteristics-and-biological-functions-of-surface-active-and-surfactant-proteins
#17
Margaret Sunde, Chi L L Pham, Ann H Kwan
Many critical biological processes take place at hydrophobic:hydrophilic interfaces, and a wide range of organisms produce surface-active proteins and peptides that reduce surface and interfacial tension and mediate growth and development at these boundaries. Microorganisms produce both small lipid-associated peptides and amphipathic proteins that allow growth across water:air boundaries, attachment to surfaces, predation, and improved bioavailability of hydrophobic substrates. Higher-order organisms produce surface-active proteins with a wide variety of functions, including the provision of protective foam environments for vulnerable reproductive stages, evaporative cooling, and gas exchange across airway membranes...
January 11, 2017: Annual Review of Biochemistry
https://www.readbyqxmd.com/read/28066954/ultrashort-self-assembling-fmoc-peptide-gelators-for-anti-infective-biomaterial-applications
#18
Alice P McCloskey, Emily R Draper, Brendan F Gilmore, Garry Laverty
Biomaterial-related infections have a significant impact on society and are a major contributor to the growing threat of antimicrobial resistance. Current licensed antibiotic classes struggle to breakdown or penetrate the exopolysaccharide biofilm barrier, resulting in sub-therapeutic concentrations of antibiotic at the surface of the biomaterial, treatment failure and increased spread of resistant isolates. This paper focuses for the first time on the ability of ultrashort Fmoc-peptide gelators to eradicate established bacterial biofilms implicated in a variety of medical device infections (Gram-positive: Staphylococcus aureus, Staphylococcus epidermidis and Gram-negative Escherichia coli, Pseudomonas aeruginosa)...
February 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28013167/influence-of-surfactin-on-physical-and-oxidative-stability-of-microemulsions-with-docosahexaenoic-acid
#19
Zhenhong He, Weiwei Zeng, Xiaoyu Zhu, Haizhen Zhao, Yingjian Lu, Zhaoxin Lu
Docosahexaenoic acid (DHA), one of the most important omega-3 polyunsaturated fatty acids (PUFAs), shows significant health benefits for human beings. In order to stabilize nutrients like DHA, microemulsion is normally used through the addition of surfactant, and surfactin as a natural peptide biosurfactant shows strong surface activity. In this study, we investigated the effects of surfactin on the stability of docosahexaenoic acid single cell oil (DHASCO) microemulsions. The microemusion region was significantly increased with elevated surfactin concentration from 0 to 0...
March 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28001086/bioactive-whey-peptide-particles-an-emerging-class-of-nutraceutical-carriers
#20
Ashkan Madadlou, Alireza Abbaspourrad
Whey-based diets have been linked with prolonged life expectancy and improved physical performance. These observations based on numerous clinical and simulated studies are attributed to diverse biological activities of whey peptides. Recently, bioactive whey peptides were exploited for enveloping nutraceuticals and drugs in view of fabricating capsules that the carrier matrix is also bioactive. Some of the most considered bioactivities of whey peptides including antihypertension, antioxidant, anti-obesity, anti-diabetes and hypocholesterolemic properties with corresponding underlying mechanisms are briefly discussed...
December 21, 2016: Critical Reviews in Food Science and Nutrition
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