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https://www.readbyqxmd.com/read/29327416/comparative-proteomics-studies-of-insect-cuticle-by-tandem-mass-spectrometry-application-of-a-novel-proteomics-approach-to-the-pea-aphid-cuticular-proteins
#1
Masson Victor, Arafah Karim, Voisin Sébastien, Bulet Philippe
The exoskeleton of arthropods, called cuticle, is a biological composite material consisting principally of N-acetylglucosamine polymer embedded in cuticular proteins (CPs). The CPs have been studied and characterized by mass spectrometry in several cuticular structures and in many arthropods. Such analyses were usually carried out by protein extraction using SDS detergent followed by electrophoresis, allowing detection and identification of a large number of CPs. To build a repertoire of cuticular structures from Bombyx mori, Apis mellifera and Anopheles gambiae we avoided the use of SDS and electrophoresis...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29313026/transcutaneous-pollinosis-immunotherapy-using-a-solid-in-oil-nanodispersion-system-carrying-t-cell-epitope-peptide-and-r848
#2
Momoko Kitaoka, Ayaka Naritomi, Yoshinori Kawabe, Masamichi Kamihira, Noriho Kamiya, Masahiro Goto
Antigen-specific immunotherapy is the only curative approach for the treatment of allergic diseases such as Japanese cedar pollinosis. Immunotherapy using a T cell epitope vaccine in combination with the adjuvant R848 is of particular interest as a safe and effective approach to treat allergic diseases. Herein, we propose a simple and easy to handle vaccine administration method using the original solid-in-oil (S/O) nanodispersion system that permeates through the skin. The S/O nanodispersion system is composed of nanoparticles of hydrophilic molecules surrounded with hydrophobic surfactants that are dispersed in an oil vehicle...
March 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/29237363/incorporation-of-an-n-methyl-amino-acid-into-a-template-peptide-alters-anisotropy-in-the-crystal-growth-of-gold-nanoparticles-synthesized-by-the-peptide-template-method
#3
Kin-Ya Tomizaki, Ryuuichi Nanbu, Makoto Kasuga, Takahito Imai
Gold nanocrystals hold promise for use as catalysts and in photonic/plasmonic devices, electronics, sensing/imaging systems, drug delivery systems, and photothermal therapies due to their unique physical, chemical, and biocompatible properties. Various organic templates have been used to control of the size, shape, structure, surface modification and topology of gold nanocrystals. The synthesis of gold nanorods currently requires a cytotoxic surfactant to control morphology. To control the shape of gold nanocrystals, we previously demonstrated the de novo design and synthesis of a b-sheet-forming nonapeptide (RU006: Ac-AIAKAXKIA-NH2, X = L-2-naphthylalanine, Nal)...
December 13, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29226249/direct-n-alkylation-of-unprotected-amino-acids-with-alcohols
#4
Tao Yan, Ben L Feringa, Katalin Barta
N-alkyl amino acids find widespread application as highly valuable, renewable building blocks. However, traditional synthesis methodologies to obtain these suffer from serious limitations, providing a major challenge to develop sustainable alternatives. We report the first powerful catalytic strategy for the direct N-alkylation of unprotected α-amino acids with alcohols. This method is highly selective, produces water as the only side product leading to a simple purification procedure, and a variety of α-amino acids are mono- or di-N-alkylated, in most cases with excellent retention of optical purity...
December 2017: Science Advances
https://www.readbyqxmd.com/read/29225158/exendin-4-partly-ameliorates-of-hyperglycemia-mediated-tissue-damage-in-lungs-of-streptozotocin-induced-diabetic-mice
#5
Fusun Oztay, Serap Sancar Bas, Selda Gezginci-Oktayoglu, Merve Ercin, Sehnaz Bolkent
Glucagon-like peptide-1 (GLP-1) stimulates insulin secretion, and plays anti-inflammatory role in atherosclerosis, and has surfactant-releasing effects in lungs. GLP-1 analogues are used in diabetes therapy. This is the first study to investigate the effects of exendin-4, a GLP-1 receptor agonist, on lung injury in diabetic mice. BALB/c male mice were divided into four groups. The first group was given only citrate buffer, the second group was given only exendin-4, the third group was given only streptozotocin (STZ), and the fourth group was given both exendin-4 and STZ...
December 7, 2017: Peptides
https://www.readbyqxmd.com/read/29206808/extrinsic-calcitonin-gene-related-peptide-inhibits-hyperoxia-induced-alveolar-epithelial-type-ii-cells-apoptosis-oxidative-stress-and-reactive-oxygen-species-ros-production-by-enhancing-notch-1-and-homocysteine-induced-endoplasmic-reticulum-protein-herp-expression
#6
Yu-Xin Bai, Fang Fang, Jia-Ling Jiang, Feng Xu
BACKGROUND Lung alveolar epithelial type II cells (AEC II) are the most important stem cells in lung tissues, which are critical for wound repair of bronchopulmonary dysplasia (BPD). This study investigated the effects of calcitonin gene-related peptide (CGRP) on AEC II cells exposed to hyperoxia. MATERIAL AND METHODS Neonatal rat AEC II cells were isolated and identified by detecting surfactant protein C (SP-C). Three small interfering RNAs targeting Notch 1 were synthesized and transfected into AEC II. A hyperoxia-exposed AEC II cell injury model was established and was divided into 8 groups...
December 5, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29172453/sample-concentration-of-charged-small-molecules-and-peptides-in-capillary-electrophoresis-by-micelle-to-cyclodextrin-stacking
#7
Joselito P Quirino, Wojciech Grochocki, Michal J Markuszewski
A stacking approach in capillary electrophoresis based on the reversal of the analytes' effective electrophoretic velocities at a dynamic stacking boundary formed between charged micelles (i.e., from long chain ionic surfactants) and neutral cyclodextrins (i.e., native λ-, β-or γ-cyclodextrin) is presented. The approach was demonstrated by the long injection of samples in a micellar solution followed by injection of a cyclodextrin solution zone, and then separation by co-electroosmotic flow capillary zone electrophoresis...
November 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29163395/role-of-soluble-innate-effector-molecules-in-pulmonary-defense-against-fungal-pathogens
#8
REVIEW
Soledad R Ordonez, Edwin J A Veldhuizen, Martin van Eijk, Henk P Haagsman
Fungal infections of the lung are life-threatening but rarely occur in healthy, immunocompetent individuals, indicating efficient clearance by pulmonary defense mechanisms. Upon inhalation, fungi will first encounter the airway surface liquid which contains several soluble effector molecules that form the first barrier of defense against fungal infections. These include host defense peptides, like LL-37 and defensins that can neutralize fungi by direct killing of the pathogen, and collectins, such as surfactant protein A and D, that can aggregate fungi and stimulate phagocytosis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29138462/the-antibacterial-and-anti-inflammatory-activity-of-chicken-cathelicidin-2-combined-with-exogenous-surfactant-for-the-treatment-of-cystic-fibrosis-associated-pathogens
#9
Brandon J H Banaschewski, Brandon Baer, Christina Arsenault, Teah Jazey, Edwin J A Veldhuizen, Johan Delport, Tracey Gooyers, James F Lewis, Henk P Haagsman, Ruud A W Veldhuizen, Cory Yamashita
Cystic fibrosis (CF) is characterized by recurrent airway infections with antibiotic-resistant bacteria and chronic inflammation. Chicken cathelicin-2 (CATH-2) has been shown to exhibit antimicrobial activity against antibiotic-resistant bacteria and to reduce inflammation. In addition, exogenous pulmonary surfactant has been suggested to enhance pulmonary drug delivery. It was hypothesized that CATH-2 when combined with an exogenous surfactant delivery vehicle, bovine lipid extract surfactant (BLES), would exhibit antimicrobial activity against CF-derived bacteria and downregulate inflammation...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29138064/bicontinuous-microemulsions-as-a-biomembrane-mimetic-system-for-melittin
#10
Douglas G Hayes, Ran Ye, Rachel N Dunlap, Divina B Anunciado, Sai Venkatesh Pingali, Hugh M O'Neill, Volker S Urban
Antimicrobial peptides effectively kill antibiotic-resistant bacteria by forming pores in prokaryotes' biomembranes via penetration into the biomembranes' interior. Bicontinuous microemulsions, consisting of interdispersed oil and water nanodomains separated by flexible surfactant monolayers, are potentially valuable for hosting membrane-associated peptides and proteins due to their thermodynamic stability, optical transparency, low viscosity, and high interfacial area. Here, we show that bicontinuous microemulsions formed by negatively-charged surfactants are a robust biomembrane mimetic system for the antimicrobial peptide melittin...
November 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29130758/proniosomes-derived-niosomes-recent-advancements-in-drug-delivery-and-targeting
#11
Maryam Khatoon, Kifayat Ullah Shah, Fakhar Ud Din, Shefaat Ullah Shah, Asim Ur Rehman, Naz Dilawar, Ahmad Nawaz Khan
Vesicular drug delivery systems have gained wide attention in the field of nanotechnology. Among them proniosomes become the superior over other vesicular carriers. Proniosomes are dry formulations of water soluble nonionic surfactant coated carrier system which immediately forms niosomes upon hydration. They have the capability to overcome the instability problems associated with niosomes and liposomes and have the potential to improve solubility, bioavailability, and absorption of various drugs. Furthermore, they offer versatile drug delivery concept for enormous number of hydrophilic and hydrophobic drugs...
2017: Drug Delivery
https://www.readbyqxmd.com/read/29121767/from-pulmonary-surfactant-synthetic-kl4-peptide-as-effective-sirna-delivery-vector-for-pulmonary-delivery
#12
Yingshan Qiu, Michael Y T Chow, Wanling Liang, Winnie W Y Chung, Judith C W Mak, Jenny K W Lam
Pulmonary delivery of small interfering RNA (siRNA) has huge potential for the treatment of a wide range of respiratory diseases. The ability of naked siRNA to transfect cells in the lungs without a delivery vector has prompted the investigation of whether an endogenous component is at least partially responsible for the cellular uptake of siRNA, and whether a safe and efficient delivery system could be developed from this component to further improve the transfection efficiency. Surfactant protein B (SP-B), a positively charged protein molecule found in lung surfactant, is one of the possible candidates...
November 17, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29116074/-proteome-of-the-human-hacat-keratinocytes-identification-of-the-oxidative-stress-proteins-after-sodium-dodecyl-sulpfate-exposur
#13
N A Petushkova, A L Rusanov, V G Zgoda, M A Pyatnitskiy, O V Larina, K V Nakhod, N G Luzgina, A V Lisitsa
Oxidative stress is a universal response of the skin cell damage of various origins. Sodium dodecyl sulfate (SDS, sodium lauryl sulfate) is an anionic surfactant commonly used as an emulsifying detergent in household cleaners. Sodium dodecyl sulfate is the reference compound for testing toxicity on cellular skin models. The effect of sodium dodecyl sulfate in sub toxic dose 25 μg/mL during 48 h on the protein profile of human keratinocytes HaCaT was studied by tandem mass spectrometry with electrospray ionization...
September 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/29096754/overview-of-ribosomal-and-non-ribosomal-antimicrobial-peptides-produced-by-gram-positive-bacteria
#14
M Tajbakhsh, A Karimi, F Fallah, M M Akhavan
The increasing incidence of antimicrobial resistance bacterial infection and decreasing effectiveness of conventional antibiotics to treatment have caused serious problems worldwide. The demand for new generationantibiotics to combat microbial pathogens is imperative. Cationic antimicrobial peptides (AMPs) with different sources from prokaryotic to complex eukaryotic organisms, with variable length, amino acid composition and secondary structure, have been consideredduring the past decades.  The advantages of large number of AMPs are related to broad spectrum and morphogenetic activities, low resistance rate among microorganismswithout side effect on human cells, rapid killing of bacteria via membrane damage and intracellular targets,and their critical roles in anti-inflammatory...
October 31, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29067798/peptide-self-assembled-nanostructures-with-distinct-morphologies-and-properties-fabricated-by-molecular-design
#15
Meiwen Cao, Sha Lu, Wenjing Zhao, Li Deng, Meng Wang, Jiqian Wang, Peng Zhou, Dong Wang, Hai Xu, Jian R Lu
Six surfactant-like peptides with the same amino acid composition but different primary sequences are designed, including G3A3V3I3K3, K3I3V3A3G3, I3V3A3G3K3, K3G3A3V3I3, V3G3I3A3K3, and K3A3I3G3V3. These peptides form antiparallel β-sheets during self-assembly. Because the constituent residues have different side chain size and hydrophobicity, sequence changes adjust group distribution and hydrophobicity on the two sides of a given β-sheet. This consequently tunes the binding energy of the side-to-side pairing conformations and leads to different self-assembled structures...
November 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29064564/directed-evolution-of-polypropylene-and-polystyrene-binding-peptides
#16
Kristin Rübsam, Lina Weber, Felix Jakob, Ulrich Schwaneberg
Surface functionalization of biological inert polymers (e.g. polypropylene PP; polystyrene PS) with material binding peptides facilitates an efficient immobilization of enzymes, bioactive peptides or antigens at ambient temperature in water. The developed robust directed evolution protocol enables to tailor polymer binding anchor peptides (PBPs) for efficient binding under application conditions. Key for a successful directed evolution campaign was to develop an epPCR protocol with a very high mutation frequency (60 mutations/kb) to ensure sufficient diversity in PBPs (47 aas LCI: "liquid chromatography peak I"; 44 aas TA2: "Tachystatin A2")...
October 24, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29044254/high-stability-of-gold-nanoparticles-towards-dna-modification-and-efficient-hybridization-via-a-surfactant-free-peptide-route
#17
Xin-Yi Li, Fu-Yan Feng, Zi-Tong Wu, Yi-Zhen Liu, Xiao-Dong Zhou, Ji-Ming Hu
With the assistance of peptides, a stable and highly efficient approach for the quick loading of thiol-DNA onto AuNPs is proposed with a high DNA utilization, which is generally applicable to specific DNA detection and diverse AuNP sizes. The maximum efficiency of hybridization reached 93% while the reaction time was shortened to 30 min.
October 18, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29040295/hydrophobic-residues-are-critical-for-the-helix-forming-hemolytic-and-bactericidal-activities-of-amphipathic-antimicrobial-peptide-tp4
#18
Ting-Wei Chang, Shu-Yi Wei, Shih-Han Wang, Hung-Mu Wei, Yu-June Wang, Chiu-Feng Wang, Chinpan Chen, You-Di Liao
Antimicrobial peptides are important components of the host innate defense mechanism against invading pathogens, especially for drug-resistant bacteria. In addition to bactericidal activity, the 25 residue peptide TP4 isolated from Nile tilapia also stimulates cell proliferation and regulates the innate immune system in mice. In this report, TP4 hyperpolarized and depolarized the membrane potential of Pseudomonas aeruginosa at sub-lethal and lethal concentrations. It also inhibited and eradicated biofilm formation...
2017: PloS One
https://www.readbyqxmd.com/read/28961531/peptide-surfactant-interactions-a-combined-spectroscopic-and-molecular-dynamics-simulation-approach
#19
Guillaume Roussel, Yves Caudano, André Matagne, Mark S Sansom, Eric A Perpète, Catherine Michaux
In the present contribution, we report a combined spectroscopic and computational approach aiming to unravel at atomic resolution the effect of the anionic SDS detergent on the structure of two model peptides, the α-helix TrpCage and the β-stranded TrpZip. A detailed characterization of the specific amino acids involved is performed. Monomeric (single molecules) and micellar SDS species differently interact with the α-helix and β-stranded peptides, emphasizing the different mechanisms occurring below and above the critical aggregation concentration (CAC)...
September 21, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28951444/quantitative-analysis-of-proteome-modulations-in-alveolar-epithelial-type-ii-cells-in-response-to-pulmonary-aspergillus-fumigatus-infection
#20
Pegah Seddigh, Thilo Bracht, Valerie Molinier-Frenkel, Flavia Castellano, Olaf Kniemeyer, Marc Schuster, Juliane Weski, Anja Hasenberg, Andreas Kraus, Gernot Poschet, Thomas Hager, Dirk Theegarte, Christiane Opitz, Axel A Brakhage, Barbara Sitek, Mike Hasenberg, Matthias Gunzer
The ubiquitous mold Aspergillus fumigatus threatens immunosuppressed patients as inducer of lethal invasive aspergillosis. A. fumigatus conidia are airborne and reach the alveoli, where they encounter alveolar epithelial cells (AEC). Previous studies reported the importance of the surfactant-producing AEC II during A. fumigatus infection via in vitro experiments using cell lines. We established a negative isolation protocol yielding untouched primary murine AEC II with a purity >90 %, allowing ex vivo analyses of the cells, which encountered the mold in vivo...
September 26, 2017: Molecular & Cellular Proteomics: MCP
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