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https://www.readbyqxmd.com/read/28733934/draft-genome-sequence-of-the-deep-sea-bacterium-moritella-sp-jt01-and-identification-of-biotechnologically-relevant-genes
#1
Robert Cardoso de Freitas, Estácio Jussie Odisi, Chiaki Kato, Marcus Adonai Castro da Silva, André Oliveira de Souza Lima
Deep-sea bacteria can produce various biotechnologically relevant enzymes due to their adaptations to high pressures and low temperatures. To identify such enzymes, we have sequenced the genome of the polycaprolactone-degrading bacterium Moritella sp. JT01, isolated from sediment samples from Japan Trench (6957 m depth), using a Illumina HiSeq2000 sequencer (12.1 million paired-end reads) and CLC Genomics Workbench (version 6.5.1) for the assembly, resulting in a 4.83-Mb genome (42 scaffolds). The genome was annotated using Rapid Annotation using Subsystem Technology (RAST), Protein Homology/analogY Recognition Engine V 2...
July 22, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28732169/design-of-modular-peptide-surfactants-and-their-surface-activity
#2
Hao-Fei Wang, David Wibowo, Zhengzhong Shao, Anton Peter J Middelberg, Chun-Xia Zhao
Designed peptide surfactants offer a number of advanced properties over conventional petrochemical surfactants, including biocompatibility, sustainability, and tailorability of the chemical and physical properties through peptide design. Their biocompatibility and degradability make them attractive for various applications, particularly for food and pharmaceutical applications. In this work, two new peptide surfactants derived from an amphiphilic peptide surfactant (AM1) were designed (AM-S and C8-AM) to better understand links between structure, interfacial activity and emulsification...
July 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28720540/formulations-and-toxicologic-in-vivo-studies-of-aqueous-cyclosporin-a-eye-drops-with-cyclodextrin-nanoparticles
#3
Sunna Jóhannsdóttir, Johannes K Kristinsson, Zoltán Fülöp, Gudrún Ásgrímsdóttir, Einar Stefánsson, Thorsteinn Loftsson
Cyclosporin A (CyA) is an immunosuppressive drug used topically to treat ocular inflammatory disorder such as dry eye disease (DES). It is a lipophilic cyclic peptide with molecular weight of 1202.6Da. The aim of this study was to develop surfactant free aqueous 0.2% (w/v) CyA eye drops where the drug is present in an aqueous vehicle containing CyA/cyclodextrin (CyA/CD) nanoparticles and then do three-month toxicological testing in rabbits. Five formulations of different CD concentrations were studied, all of them contained 12...
July 15, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28705615/transcutaneous-immunotherapy-of-pollinosis-using-solid-in-oil-nanodispersions-loaded-with-t-cell-epitope-peptides
#4
Qingliang Kong, Momoko Kitaoka, Rie Wakabayashi, Noriho Kamiya, Masahiro Goto
Pollinosis, a typical seasonal allergy, is a serious public health problem. Limited numbers of patients receive curative immunotherapy instead of symptomatic therapy; however, there are still some concerns about the inconvenience and side effects of subcutaneous injections and sublingual administration caused by immunotherapy. Here, we propose a simple and safe transcutaneous immunotherapy using solid-in-oil (S/O) nanodispersions loaded with vaccine T cell epitope peptides derived from pollen allergen. S/O nanodispersions are oil-based dispersions of antigens coated with hydrophobic surfactants...
July 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28684364/polyarginine-nanocapsules-a-versatile-nanocarrier-with-potential-in-transmucosal-drug-delivery
#5
Ana Gonzalez-Paredes, Dolores Torres, María José Alonso
The objective of this work was to investigate the potential utility of nanocapsules composed of an oily core decorated with a single polyarginine (PARG), or double PARG/polyacrylic acid (PAA) layer as oral peptide delivery carrier. A step-by-step formulation optimization process was designed, which involved the study of the influence of the surfactants, oils and polymer shells (PARG of different molecular weight and PAA) on the nanocapsules physicochemical properties, peptide loading efficiency, stability in simulated intestinal fluids (SIF) and capacity to enhance the permeability of the intestinal epithelium...
July 3, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28643235/design-and-evaluation-of-rgd-modified-gemini-surfactant-based-lipoplexes-for-targeted-gene-therapy-in-melanoma-model
#6
Waleed Mohammed-Saeid, Jackson Chitanda, Mays Al-Dulaymi, Ronald Verrall, Ildiko Badea
PURPOSE: We have developed and evaluated novel peptide-targeted gemini surfactant-based lipoplexes designed for melanoma gene therapy. METHODS: Integrin receptor targeting peptide, cyclic-arginylglycylaspartic acid (cRGD), was either chemically coupled to a gemini surfactant backbone or physically co-formulated with lipoplexes. Several formulations and transfection techniques were developed. Transfection efficiency and cellular toxicity of the lipoplexes were evaluated in an in vitro human melanoma model...
June 22, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28623702/adsorption-of-ionic-surfactants-at-microscopic-air-water-interfaces-using-the-micropipette-interfacial-area-expansion-method-measurement-of-the-diffusion-coefficient-and-renormalization-of-the-mean-ionic-activity-for-sds
#7
Koji Kinoshita, Elisa Parra, David Needham
The dynamic adsorption of ionic surfactants at air-water interfaces have been less-well studied than that of the simpler non-ionics since experimental limitations on dynamic surface tension (DST) measurements create inconsistencies in their kinetic analysis. Using our newly designed "Micropipette interfacial area-expansion method", we have measured and evaluated both equilibrium and dynamic adsorption of a well-known anionic surfactant, sodium dodecyl sulphate (SDS), in the absence or presence of 100mM NaCl...
May 25, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28605904/kalata-b1-and-kalata-b2-have-a-surfactant-like-activity-in-phosphatidylethanolomine-containing-lipid-membranes
#8
Charles G Cranfield, Sónia Troeira Henriques, Boris Martinac, Paul Duckworth, David J Craik, Bruce Cornell
Cyclotides are cyclic disulfide-rich peptides that are chemically and thermally stable and possess pharmaceutical and insecticidal properties. The activities reported for cyclotides correlate with their ability to target phosphatidylethanolamine (PE)-phospholipids and disrupt cell membranes. However, the mechanism by which this disruption occurs remains unclear. In the current study we examine the effect of the prototypic cyclotides, kalata B1 (kB1) and kalata B2 (kB2), on tethered lipid bilayer membranes (tBLMs) using swept frequency electrical impedance spectroscopy...
June 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28594046/balancing-the-intermolecular-forces-in-peptide-amphiphiles-for-controlling-self-assembly-transitions
#9
C J Buettner, A J Wallace, S Ok, A A Manos, M J Nicholl, A Ghosh, M F Tweedle, J E Goldberger
While the influence of alkyl chain length and headgroup size on self-assembly behaviour has been well-established for simple surfactants, the rational control over the pH- and concentration-dependent self-assembly behaviour in stimuli responsive peptides remains an elusive goal. Here, we show that different amphiphilic peptides can have similar self-assembly phase diagrams, providing the relative strengths of the attractive and repulsive forces are balanced. Using palmitoyl-YYAAEEEEK(DO3A:Gd)-NH2 and palmitoyl-YAAEEEEK(DO3A:Gd)-NH2 as controls, we show that reducing hydrophobic attractive forces through fewer methylene groups in the alkyl chain will lead to a similar self-assembly phase diagram as increasing the electrostatic repulsive forces via the addition of a glutamic acid residue...
June 21, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28570277/epithelial-gpr116-regulates-pulmonary-alveolar-homeostasis-via-gq-11-signaling
#10
Kari Brown, Alyssa Filuta, Marie-Gabrielle Ludwig, Klaus Seuwen, Julian Jaros, Solange Vidal, Kavisha Arora, Anjaparavanda P Naren, Kathirvel Kandasamy, Kaushik Parthasarathi, Stefan Offermanns, Robert J Mason, William E Miller, Jeffrey A Whitsett, James P Bridges
Pulmonary function is dependent upon the precise regulation of alveolar surfactant. Alterations in pulmonary surfactant concentrations or function impair ventilation and cause tissue injury. Identification of the molecular pathways that sense and regulate endogenous alveolar surfactant concentrations, coupled with the ability to pharmacologically modulate them both positively and negatively, would be a major therapeutic advance for patients with acute and chronic lung diseases caused by disruption of surfactant homeostasis...
June 2, 2017: JCI Insight
https://www.readbyqxmd.com/read/28570040/self-assembly-of-mesoscopic-peptide-surfactant-fibrils-investigated-by-storm-super-resolution-fluorescence-microscopy
#11
Henry Cox, Pantelis Georgiades, Hai Xu, Thomas A Waigh, Jian R Lu
Super-resolution fluorescence microscopy, specifically stochastic reconstruction microscopy (STORM), and atomic force microscopy (AFM) were used to image the self-assembly processes of the peptide surfactant I3K. The peptide surfactants self-assembled into giant helical fibrils with diameters between 5 and 10 nm with significant helical twisting. The resolution of the STORM images was 30 nm, calculated using the Fourier ring correlation method. STORM compares favorably with AFM for the calculation of contour lengths (∼6 μm) and persistence lengths (10...
June 26, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28560564/an-electrospray-ionization-mass-spectrometry-study-on-the-in-vacuo-hetero-oligomers-formed-by-the-antimicrobial-peptides-surfactin-and-gramicidin-s
#12
Marina Rautenbach, N Maré Vlok, Hans A Eyéghé-Bickong, Marthinus J van der Merwe, Marietjie A Stander
It was previously observed that the lipopeptide surfactants in surfactin (Srf) have an antagonistic action towards the highly potent antimicrobial cyclodecapeptide, gramicidin S (GS). This study reports on some of the molecular aspects of the antagonism as investigated through complementary electrospray ionization mass spectrometry techniques. We were able to detect stable 1:1 and 2:1 hetero-oligomers in a mixture of surfactin and gramicidin S. The noncovalent interaction between GS and Srf, with the proposed equilibrium: GS~Srf↔GS+Srf correlated to apparent K d values of 6-9 μM in gas-phase and 1 μM in aqueous solution...
August 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28541037/phase-behavior-of-salt-free-polyelectrolyte-gel-surfactant-systems
#13
Martin Andersson, Per Hansson
Ionic surfactants tend to collapse the outer parts of polyelectrolyte gels, forming shells that can be used to encapsulate other species including protein and peptide drugs. In this paper, the aqueous phase behavior of covalently cross-linked polyacrylate networks containing sodium ions and dodecyltrimethylammonium ions as counterions is investigated by means of swelling isotherms, dye staining, small-angle X-ray scattering, and confocal Raman spectroscopy. The equilibrium state is approached by letting the networks absorb pure water...
June 8, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28540997/a-combination-of-short-and-simple-surfactant-protein-b-and-c-analogues-as-a-new-synthetic-surfactant-in-vitro-and-animal-experiments
#14
Yong Sung Choi, Sung Hoon Chung, Chong Woo Bae
PURPOSE: Pulmonary surfactants for preterm infants contain mostly animal-derived surfactant proteins (SPs), which are essential for lowering surface tension. We prepared artificial pulmonary surfactants using synthetic human SP analogs and performed in vitro and in vivo experiments. MATERIALS AND METHODS: We synthesized peptide analogues that resemble human SP-B (RMLPQLVCRLVLRCSMD) and SP-C (CPVHLKRLLLLLLLLLLLLLLLL). Dipalmitoylphosphatidylcholine (DPPC), phosphatidylglycerol (PG), and palmitic acid (PA) were added and mixed in lyophilized to render powdered surfactant...
July 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28534479/efficient-protein-production-inspired-by-how-spiders-make-silk
#15
Nina Kronqvist, Médoune Sarr, Anton Lindqvist, Kerstin Nordling, Martins Otikovs, Luca Venturi, Barbara Pioselli, Pasi Purhonen, Michael Landreh, Henrik Biverstål, Zigmantas Toleikis, Lisa Sjöberg, Carol V Robinson, Nicola Pelizzi, Hans Jörnvall, Hans Hebert, Kristaps Jaudzems, Tore Curstedt, Anna Rising, Jan Johansson
Membrane proteins are targets of most available pharmaceuticals, but they are difficult to produce recombinantly, like many other aggregation-prone proteins. Spiders can produce silk proteins at huge concentrations by sequestering their aggregation-prone regions in micellar structures, where the very soluble N-terminal domain (NT) forms the shell. We hypothesize that fusion to NT could similarly solubilize non-spidroin proteins, and design a charge-reversed mutant (NT*) that is pH insensitive, stabilized and hypersoluble compared to wild-type NT...
May 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28508363/glycoproteins-enrichment-and-lc-ms-ms-glycoproteomics-in-central-nervous-system-applications
#16
Rui Zhu, Ehwang Song, Ahmed Hussein, Firas H Kobeissy, Yehia Mechref
Proteins and glycoproteins play important biological roles in central nervous systems (CNS). Qualitative and quantitative evaluation of proteins and glycoproteins expression in CNS is critical to reveal the inherent biomolecular mechanism of CNS diseases. This chapter describes proteomic and glycoproteomic approaches based on liquid chromatography/tandem mass spectrometry (LC-MS or LC-MS/MS) for the qualitative and quantitative assessment of proteins and glycoproteins expressed in CNS. Proteins and glycoproteins, extracted by a mass spectrometry friendly surfactant from CNS samples, were subjected to enzymatic (tryptic) digestion and three down-stream analyses: (1) a nano LC system coupled with a high-resolution MS instrument to achieve qualitative proteomic profile, (2) a nano LC system combined with a triple quadrupole MS to quantify identified proteins, and (3) glycoprotein enrichment prior to LC-MS/MS analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28503550/design-of-surfactant-protein-b-peptide-mimics-based-on-the-saposin-fold-for-synthetic-lung-surfactants
#17
Frans J Walther, Larry M Gordon, Alan J Waring
Surfactant protein (SP)-B is a 79-residue polypeptide crucial for the biophysical and physiological function of endogenous lung surfactant. SP-B is a member of the Saposin or Saposin-like proteins (SAPLIP) family of proteins that share an overall three-dimensional folding pattern based on secondary structures and disulfide connectivity and exhibit a wide diversity of biological functions. Here we review the synthesis, molecular biophysics and activity of synthetic analogs of Saposin proteins designed to mimic those interactions of the parent proteins with lipids that enhance interfacial activity...
September 2016: Biomedicine Hub
https://www.readbyqxmd.com/read/28493723/influence-of-conventional-surfactants-on-the-self-assembly-of-a-bola-type-amphiphilic-peptide
#18
Yueying Cao, Dong Wang, Peng Zhou, Yurong Zhao, Yawei Sun, Jiqian Wang
Structural and morphological regulation is a distinctly important topic in peptide self-assembly, and is also regarded as the fundamental point in peptide-based biomaterials development. In this paper, we showed that adding anionic surfactant SDS to a bola amphiphilic peptide KI4K could result in the reconstruction of β-sheet secondary structure besides the changes in self-assembly morphologies from nanotubes to helical ribbons, nanofibers, or straight nanotapes according to the negatively stained transmission electron microscopy, atomic force microscopy, circular dichroism spectroscopy, and Fourier transform infrared spectroscopy results...
June 6, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28490071/recovery-of-a-bacteriocin-like-inhibitory-substance-from-pediococcus-acidilactici-kp10-using-surfactant-precipitation
#19
Fadzlie Wong Faizal Wong, Arbakariya B Ariff, Sahar Abbasiliasi, David C Stuckey
Bacteriocin is an important peptide which can be used as an anti-microbial agent in food. However, simpler and more cost-effective purification methods need to be developed compared to chromatography to enhance its commercial viability. Surfactant precipitation was employed for the first time to purify bacteriocin-like inhibitory substance (BLIS) from a fermentation broth of Pediococcus acidilactici Kp10, and the amount precipitated was investigated as a function of anionic surfactant (AOT) concentration, and pH...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28482785/microemulsions-for-protein-delivery-loading-optimization-and-stability
#20
Diego R Perinelli, Marco Cespi, Stefania Pucciarelli, Silvia Vincenzetti, Luca Casettari, Jenny K W Lam, Serena Logrippo, Elisa Canala, Mahmoud E Soliman, Giulia Bonacucina, Giovanni F Palmieri
BACKGROUND: Microemulsions are attractive delivery systems for therapeutic proteins and peptides due to their ability to enhance bioavailability. Although different proteins and peptides have been successfully delivered through such ternary systems, no information can be found about protein loading and the formulation stability when such microemulsions are prepared with pharmaceutically-approved oils and surfactants. The aim of this work was to optimise a ternary system consisting of water/ethyl oleate/Span 80-Tween 80 and to determine its protein loading capacity and stability, using bovine serum albumin (BSA) as a model of biomolecule...
May 7, 2017: Current Pharmaceutical Biotechnology
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