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https://www.readbyqxmd.com/read/28438316/multiple-modes-of-capillary-electrophoresis-applied-in-peptide-nucleic-acid-related-study
#1
Xiaoqian Wang, Youhao Hu, Feng Qu, Rizwan Ullah Khan
Peptide Nucleic Acid (PNA) is a nucleic acid analogue, whose neutrally charged and hydrophobic backbone makes it more stable in vivo, so it might act as a potentially better protein probe as compared to aptamer. Currently the investigation of PNA and protein interaction is scarce. In this research, multiple modes of capillary electrophoresis were established and applied for PNA characterization and its interaction with ssDNA and protein. A 15-mer PNA having the same nucleobase sequence as 15-mer anti-thrombin DNA aptamer was chosen as PNA model for this study, its pI (7...
April 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28437109/structure-of-the-fundamental-lipopeptide-surfactin-at-the-air-water-interface-investigated-by-sum-frequency-generation-spectroscopy
#2
Sami Alhassan Goussous, Michael Thomas Lee Casford, Annabel C Murphy, George Salmond, Finian J Leeper, Paul B Davies
The lipopeptide surfactin produced by certain strains of Bacillus subtilis is a powerful bio-surfactant possessing potentially useful antimicrobial properties. In order to better understand its surface behaviour, we have used surface sensitive Sum Frequency Generation (SFG) vibrational spectroscopy in the C-H and C=O stretching regions to determine its structure at the air/water interface. Using surfactin with the leucine groups of the peptide ring perdeuterated we have shown that the majority of the SFG signals arise from the 4 leucine residues...
April 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28413467/calcitonin-gene-related-peptide-protects-type-ii-alveolar-epithelial-cells-from-hyperoxia-induced-dna-damage-and-cell-death
#3
Hongmin Fu, Tiesong Zhang, Rongwei Huang, Zhen Yang, Chunming Liu, Ming Li, Fang Fang, Feng Xu
Hyperoxia therapy for acute lung injury (ALI) may unexpectedly lead to reactive oxygen species (ROS) production and cause additional ALI. Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide that regulates inflammasome activation. However, the role of CGRP in DNA damage during hyperoxia is unclear. Therefore, the aim of the present study was to investigate the effects of CGRP on DNA damage and the cell death of alveolar epithelial type II cells (AEC II) exposed to 60% oxygen. AEC II were isolated from 19-20 gestational day fetal rat lungs and were exposed to air or to 60% oxygen during treatment with CGRP or the specific CGRP receptor antagonist CGRP8-37...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28412812/an-ionstar-experimental-strategy-for-ms1-ion-current-based-quantification-using-ultra-high-field-orbitrap-reproducible-in-depth-and-accurate-protein-measurement-in-larger-cohorts
#4
Xiaomeng Shen, Shichen Shen, Jun Li, Qiang Hu, Lei Nie, Chengjian Tu, Xue Wang, Benjamin Orsburn, Jianmin Wang, Jun Qu
In-depth and reproducible protein measurement in many biological samples is often critical for pharmaceutical/biomedical proteomics but remains challenging. MS1-based quantification using quadrupole/ultra-high-field Orbitrap(Q/UHF-Orbitrap) holds great promise, but the critically important experimental approaches enabling reliable large-cohort analysis have long been overlooked. Here we described an IonStar experimental strategy achieving excellent quantitative quality of MS1 quantification. Key features include: i)an optimized, surfactant-aided sample preparation approach provides highly efficient(>75%recovery) and reproducible(<15%CV) peptide recovery across large cell/tissue cohorts; ii)a long column with modest gradient length (e...
April 16, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28409852/tandem-mass-spectrometric-analysis-of-novel-peptide-modified-gemini-surfactants-used-as-gene-delivery-vectors
#5
Mays Al-Dulaymi, Anas El-Aneed
Diquaternary ammonium gemini Surfactants have emerged as effective gene delivery vectors. A novel series of eleven peptide-modified compounds was synthesized, showing promising results in delivering genetic materials. The purpose of this work is to elucidate the tandem mass spectrometric (MS/MS) dissociation behaviour of these novel molecules establishing a generalized MS/MS fingerprint. Exact mass measurements were achieved using a hybrid quadrupole orthogonal time-of-flight mass spectrometer (QqToF-MS) and a multi-stage tandem mass spectrometric analysis was conducted using a triple quadrupole-linear ion trap mass spectrometer (QqQLIT-MS)...
April 14, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28359959/designing-new-surfactant-peptides-for-binding-to-carbon-nanotubes-via-computational-approaches
#6
Alireza Mansouri, Karim Mahnam
The non-covalent interaction between single-walled carbon nanotube and surfactant peptides makes them soluble in biological media to be used in nano-medicine, drug delivery and gene therapy. Pervious study has shown that two important parameters in binding peptides into nanotubes are hydrophobic effect and the number of aromatic amino acids. Ten surfactant peptides with the length of eight residue, including Lys, Trp, Tyr, Phe and Val, were designed to investigate the important parameters in binding peptides to a (6, 6) carbon nanotube...
March 14, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28347848/comparison-of-the-protective-effect-of-self-emulsifying-peptide-drug-delivery-systems-towards-intestinal-proteases-and-glutathione
#7
Gergely Hetényi, Janine Griesser, Michael Moser, Frédéric Demarne, Vincent Jannin, Andreas Bernkop-Schnürch
AIM: The aim of this study was to evaluate the protective effect of self-emulsifying drug delivery systems (SEDDS) for therapeutic peptides towards intestinal proteases and reduced glutathione (GSH). METHODS: Sodium docusate was applied as anionic surfactant for hydrophobic ion pairing with leuprorelin (LEU), insulin (INS) and desmopressin (DES). The complexes were loaded into SEDDS that were characterized regarding droplet size distribution and zeta potential. The release profile of the peptides was examined by dialysis membrane method...
March 24, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28330122/production-purification-and-characterization-of-a-thermotolerant-alkaline-serine-protease-from-a-novel-species-bacillus-caseinilyticus
#8
Thirumala Mothe, Vishnuvardhan Reddy Sultanpuram
Alkaline proteases are important enzymes in many industrial applications, especially as additives in laundry detergent industry. Though there are a number of Bacillus species which are reported to be producing proteases, the efficiency of a protease produced by a novel strain has to be studied in comparison to the others. Hence, in this study, an alkaline serine protease produced by a novel species Bacillus caseinilyticus was purified and characterized for its possible usage in detergent industry. Ammonium sulphate, dialysis and DEAE column chromatographic methods were used for purification of the isolated alkaline protease...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28315219/assessment-of-ige-reactivity-of-%C3%AE-casein-by-western-blotting-after-digestion-with-simulated-gastric-fluid
#9
Sara Benedé, Rosina López-Fandiño, Elena Molina
Cow's milk allergy is defined as an immunologically mediated adverse reaction to cow's milk proteins and it is usually, along with hen's egg allergy, the first food allergy identified in childhood.One of the main aspects to consider when evaluating the allergenic potential of food proteins is the effect of gastric digestion. It is known that allergens are usually able to survive the harsh acidic environment of the stomach, tolerate the presence of surfactants, and resist digestion by pepsin. They might also be digested into high molecular weight peptide fragments, which retain the same, or sometimes increased, IgE-binding...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28291369/ultrashort-single-walled-carbon-nanotubes-insert-into-a-pulmonary-surfactant-monolayer-via-self-rotation-poration-and-mechanical-inhibition
#10
Tongtao Yue, Yan Xu, Shixin Li, Zhen Luo, Xianren Zhang, Fang Huang
It has been widely accepted that longer single-walled carbon nanotubes (SWCNTs) exhibit higher toxicity by causing severe pneumonia once inhaled, yet relatively little is known regarding the potential toxicity of ultrashort SWCNTs, which are of central importance to the development of suitable vehicles for biomedical applications. Here, by combining coarse-grained molecular dynamics (CGMD), pulling simulations, and scaling analysis, we demonstrate that the inhalation toxicity of ultrashort SWCNTs (1.5 nm < l < 5...
March 22, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28288148/natriuretic-peptide-c-receptor-in-the-developing-sheep-lung-role-in-perinatal-transition
#11
Bobby Mathew, Christopher A D'Angelis, Satyan Lakshminrusimha, Peter A Nickerson, June J Sokolowski, Vasantha H S Kumar, Huamei Wang, Karen A Wynn, Bruce A Holm, Rita M Ryan
BACKGROUND: At birth, the release of surfactant from Alveolar Type II (ATII) cells is stimulated by increased activity of the beta-adrenergic/adenylyl cyclase/cAMP- signaling cascade. Atrial natriuretic peptide (ANP) stimulates surfactant secretion through Natriuretic peptide receptor A (NPR-A). ANP inhibits adenylyl cyclase activity through its binding to NPR-C. We wished to further understand the role of the NPR-C in perinatal transition. METHODS: We studied fetal and newborn sheep ATII expression of NPR-C and surfactant secretion in isolated ATIIs by measuring (3)[H] choline release into the media...
March 13, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28282112/peptide-cross-linkers-immobilization-of-platinum-nanoparticles-highly-dispersed-on-graphene-oxide-nanosheets-with-enhanced-photocatalytic-activities
#12
Tsukasa Mizutaru, Galina Marzun, Sebastian Kohsakowski, Stephan Barcikowski, Dachao Hong, Hiroaki Kotani, Takahiko Kojima, Takahiro Kondo, Junji Nakamura, Yohei Yamamoto
For exerting potential catalytic and photocatalytic activities of metal nanoparticles (MNPs), immobilization of MNPs on a support medium in highly dispersed state is desired. In this Research Article, we demonstrated that surfactant-free platinum nanoparticles (PtNPs) were efficiently immobilized on graphene oxide (GO) nanosheets in a highly dispersed state by utilizing oligopeptide β-sheets as a cross-linker. The fluorenyl-substituted peptides were designed to form β-sheets, where metal-binding thiol groups and protonated and positively charged amino groups are integrated on the opposite sides of the surface of a β-sheet, which efficiently bridge PtNPs and GO nanosheet...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28277006/cationic-copolymer-augments-membrane-permeabilizing-activity-of-an-amphiphilic-peptide
#13
Wakako Sakamoto, Takuro Ochiai, Naohiko Shimada, Atsushi Maruyama
Membrane disruptive peptides (also called membrane fusogenic peptides) have been employed for cytosolic delivery of macromolecules such as nucleic acids and proteins. We reported previously that the cationic graft copolymer, poly(allylamine)-graft-dextran (PAA-g-Dex), augments membrane disruptive activity of the negatively charged E5 peptide. Strong membrane disruptive activity was observed in the presence of the copolymer at both acidic and neutral pH. In this paper, activities of E5/PAA-g-Dex mixture were further explored...
February 17, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28271630/sample-clean-up-strategies-for-electrospray-ionization-mass-spectrometry-applications-in-bottom-up-proteomics-trends-from-2012-2016
#14
REVIEW
Ria Marni Tubaon, Paul R Haddad, Joselito P Quirino
Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favoured over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sample or produced during tryptic digestion are detrimental in MS analysis and affect the proteome data, thus sample preparation for removal of these unwanted components has become essential. Here, we review 48 research papers on strategies for removal of salts and surfactants (in particular, sodium dodecyl sulfate, SDS) prior to electrospray ionization (ESI)-MS analysis in bottom-up proteomics from 2012-2016...
March 8, 2017: Proteomics
https://www.readbyqxmd.com/read/28254280/effects-of-alkyl-polyglycoside-apg-on-bombyx-mori-silk-degumming-and-the-mechanical-properties-of-silk-fibroin-fibre
#15
Fei Wang, Yu-Qing Zhang
Alkyl polyglycoside (APG), a nonionic surfactant, is often considered to be a green surfactant and is synthesized using glucose and long chain fatty alcohols. It is used as a degumming agent of Bombyx mori silk fibre in this study for the first time. We studied APG systematically in comparison to the traditional degumming methods, such as aqueous solutions of sodium carbonate (Na2CO3) and neutral soap (NS). After repeatedly boiling silk fibres in an aqueous solution of 0.25% APG three times for 30min and using a bath ratio of 1:90-120 (g/mL), sericin was completely removed from the fibre...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28235590/rational-design-of-polyarginine-nanocapsules-intended-to-help-peptides-overcoming-intestinal-barriers
#16
Zhigao Niu, Erik Tedesco, Federico Benetti, Aloïse Mabondzo, Isabella Monia Montagner, Ilaria Marigo, David Gonzalez-Touceda, Sulay Tovar, Carlos Diéguez, Manuel J Santander-Ortega, María J Alonso
The aim of this work was to rationally design and characterize nanocapsules (NCs) composed of an oily core and a polyarginine (PARG) shell, intended for oral peptide delivery. The cationic polyaminoacid, PARG, and the oily core components were selected based on their penetration enhancing properties. Insulin was adopted as a model peptide to assess the performance of the NCs. After screening numerous formulation variables, including different oils and surfactants, we defined a composition consisting of oleic acid, sodium deoxycholate (SDC) and Span 80...
February 21, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28221717/cerebral-and-lung-effects-of-a-new-generation-synthetic-surfactant-with-sp-b-and-sp-c-analogs-in-preterm-lambs
#17
Carmen Rey-Santano, Victoria E Mielgo, Xabier Murgia, Miguel Angel Gomez-Solaetxe, Fabrizio Salomone, Federico Bianco, Nicola Pelizzi, Begoña Loureiro, Jon López de Heredia Y Goya
BACKGROUND: Though natural surfactants (SF) are clinically superior to protein-free synthetic preparations, CHF-5633, a synthetic SF containing SP-B and SP-C analog peptides is a potential alternative to natural SF for treating neonatal respiratory distress syndrome (RDS). Nevertheless, information is lacking regarding the safety of this new treatment for the neonatal brain. We sought to compare the cerebral and pulmonary effects of this new synthetic surfactant (CHF5633) with those of natural porcine surfactant (Cursosurf) in premature lambs with RDS...
February 21, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28191927/foldectures-3d-molecular-architectures-from-self-assembly-of-peptide-foldamers
#18
Sung Hyun Yoo, Hee-Seung Lee
The wide range of fascinating supramolecular architectures found in nature, from DNA double helices to giant protein shells, inspires researchers to mimic the diverse shapes and functions of natural systems. Thus, a variety of artificial molecular platforms have been developed by assembling DNA-, peptide-, and protein-based building blocks for medicinal and biological applications. There has also been a significant interest in the research of non-natural oligomers (i.e., foldamers) that fold into well-defined secondary structures analogous to those found in proteins, because the assemblies of foldamers are expected not only to form biomimetic supramolecular architectures that resemble those of nature but also to display unique functions and unprecedented topologies at the same time due to their different folding propensities from those of natural building blocks...
February 13, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28188875/hydrophobic-ion-pairing-key-to-highly-payloaded-self-emulsifying-peptide-drug-delivery-systems
#19
Janine Griesser, Gergely Hetényi, Michael Moser, Frédéric Demarne, Vincent Jannin, Andreas Bernkop-Schnürch
AIM: The aim of this study was the formation and characterization of various ion pairs of therapeutic peptides with different surfactants in order to reach a high payload in self-emulsifying drug delivering systems (SEDDS). METHODS: Hydrophobic ion pairs (HIP) were formed between the anionic surfactants sodium docusate, dodecylsulfate and oleate and the peptides leuprorelin (LEU), insulin (INS) and desmopressin (DES). The efficiency of HIP formation was evaluated by quantifying the amount of formed complexes, log P value determination in n-octanol/water via HPLC and zeta potential measurements...
February 7, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28188794/surfactant-protein-a-sp-a-and-sp-a-derived-peptide-attenuate-chemotaxis-of-mast-cells-induced-by-human-%C3%AE-defensin-3
#20
Yasuaki Uehara, Motoko Takahashi, Masaki Murata, Atsushi Saito, Rina Takamiya, Yoshihiro Hasegawa, Koji Kuronuma, Hirofumi Chiba, Jiro Hashimoto, Norimasa Sawada, Hiroki Takahashi, Yoshio Kuroki, Shigeru Ariki
Human β-defensin 3 (hBD3) is known to be involved in mast cell activation. However, molecular mechanisms underlying the regulation of hBD3-induced mast cell activation have been poorly understood. We previously reported that SP-A and SP-A-derived peptide 01 (SAP01) regulate the function of hBD3. In this study, we focused on the effects of SP-A and SAP01 on the activation of mast cells induced by hBD3. SAP01 directly bound to hBD3. Mast cell-mediated vascular permeability and edema in hBD3 administered rat ears were decreased when injected with SP-A or SAP01...
March 25, 2017: Biochemical and Biophysical Research Communications
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