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https://www.readbyqxmd.com/read/28634057/evaluation-of-the-biotechnological-potential-of-a-novel-purified-protease-bs1-from-bacillus-safensis-s406-on-the-chitin-extraction-and-detergent-formulation
#1
Samiha Mhamdi, Intidhar Bkhairia, Rim Nasri, Tahar Mechichi, Moncef Nasri, Alya Sellami Kamoun
The aim of this study is the production of High-Added Value enzyme for Industrial Uses. An extracellular alkaline stable protease BS1 was purified from a newly bacterium, Bacillus safensis S406, isolated from the Sfax Solar Saltern. BS1 was purified by a process involving sulfate d'ammonium sulfate precipitation, sephadex G-75, Mono-Q anion-exchange chromatography and ultrafiltation. The enzyme has a molecular weight of 24kDa and appeared as a single band on native-PAGE. The optimum pH and temperature values of its proteolytic activity were pH 11...
June 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28631792/nanoscopic-dynamics-of-bicontinous-microemulsions-effect-of-membrane-associated-protein
#2
V K Sharma, Douglas G Hayes, Volker S Urban, Hugh M O'Neill, M Tyagi, E Mamontov
Bicontinous microemulsions (BμE) generally consist of nanodomains formed by surfactant in a mixture of water and oil at nearly equal proportions and are potential candidates for the solubilization and purification of membrane proteins. Here we present the first time report of nanoscopic dynamics of surfactant monolayers within BμEs formed by the anionic surfactant sodium dodecyl sulfate (SDS) measured on the nanosecond to picosecond time scale using quasielastic neutron scattering (QENS). BμEs investigated herein consisted of middle phases isolated from Winsor-III microemulsion systems that were formed by mixing aqueous and oil solutions under optimal conditions...
June 20, 2017: Soft Matter
https://www.readbyqxmd.com/read/28628923/cremophor-el-alters-the-plasma-protein-binding-and-pharmacokinetic-profile-of-valspodar-in-rats
#3
Ziyad Binkhathlan
Cremophor EL is a nonionic surfactant widely used in pharmaceutical formulations. Nonetheless, there are several reports on the influence of this excipient on the protein binding, pharmacokinetics, and pharmacodynamics of drugs. Valspodar is an investigational non-immunosuppressive derivative of cyclosporine A, used in clinical trials for treatment of multidrug resistant tumors. The formulation of valspodar (Amdray(®)) contains cremophor EL and ethanol as solubilizing agents. The main aim of the current study was to assess the plasma protein binding (in vitro) and the pharmacokinetic profile of valspodar in the cremophor EL-based formulation in comparison to a cremophor EL-free formulation following intravenous (i...
June 19, 2017: Drug Research
https://www.readbyqxmd.com/read/28624607/obese-asthmatics-have-decreased-surfactant-protein-a-levels-mechanisms-and-implications
#4
Njira Lugogo, Dave Francisco, Kenneth J Addison, Akarsh Manne, William Pederson, Jennifer L Ingram, Cynthia L Green, Benjamin T Suratt, James J Lee, Mary E Sunday, Monica Kraft, Julie G Ledford
BACKGROUND: Eosinophils are prominent in some individuals with asthma and are elevated in the submucosa in a subgroup of obese asthmatics. Surfactant protein-A (SP-A) modulates host responses to infectious and environmental insults. OBJECTIVE: To determine if SP-A levels are altered in obese asthma patients compared to a control group and to determine the implications of these alterations in SP-A levels in asthma. METHODS: Bronchoalveolar lavage fluid from 23 lean, 12 overweight and 20 obese subjects were examined for SP-A...
June 14, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28623702/adsorption-of-ionic-surfactants-at-microscopic-air-water-interfaces-using-the-micropipette-interfacial-area-expansion-method-measurement-of-the-diffusion-coefficient-and-renormalization-of-the-mean-ionic-activity-for-sds
#5
Koji Kinoshita, Elisa Parra, David Needham
The dynamic adsorption of ionic surfactants at air-water interfaces have been less-well studied than that of the simpler non-ionics since experimental limitations on dynamic surface tension (DST) measurements create inconsistencies in their kinetic analysis. Using our newly designed "Micropipette interfacial area-expansion method", we have measured and evaluated both equilibrium and dynamic adsorption of a well-known anionic surfactant, sodium dodecyl sulphate (SDS), in the absence or presence of 100mM NaCl...
May 25, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28623690/candidate-cytochrome-p450-genes-for-ethoxyresorufin-o-deethylase-activity-in-oyster-crassostrea-gigas
#6
Marília Nardelli Siebert, Jacó Joaquim Mattos, Guilherme Toledo-Silva, Guilherme Razzera, Afonso Celso Dias Bainy
Vertebrate cytochrome P450 1 (CYP1) enzymes metabolize endogenous and xenobiotic compounds and usually demonstrate a substrate-inducible response. Ethoxyresorufin O-deethylase activity (EROD) is a common method to quantify CYP1 enzymes activity in these organisms. Despite the absence of this gene family in protostomes, CYP1-like genes were identified in several species, even though no evolutionary relationship has been established with the vertebrate CYP1 family. In the present study, EROD activity was evaluated in microsomal fraction of gills, digestive gland and mantle of Crassostrea gigas...
June 9, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28622544/conformational-transition-of-%C3%AE%C2%BA-casein-in-micellar-environment-insight-from-the-tryptophan-fluorescence
#7
Smruti Mishra, Geetanjali Meher, Hirak Chakraborty
Intrinsically disordered proteins (IDPs) are under intense analysis due to their structural flexibility and importance in biological functions. Minuscule modulation in the microenvironment induces significant conformational changes in IDPs, and these non-native conformations of the IDPs often induce aggregation and cause cell death. Changes in the membrane composition often change the microenvironment, which promote conformational change and aggregation of IDPs. κ-Casein, an important milk protein, belongs to the class of IDPs containing net negative charges...
June 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28620154/influence-of-donor-lung-surfactant-a-and-b-protein-expression-on-the-development-of-primary-graft-dysfunction-after-lung-transplantation-a-pilot-study
#8
Asmae Belhaj, Carine Boven, Laurence Dewachter, Maria Ruiz Patino, Youri Sokolow, Benoît Rondelet
BACKGROUND Primary graft dysfunction (PGD) is responsible of high early mortality in lung transplanted patients. We measured the rate of surfactant proteins in the organ donor, and we observed the occurrence of lung PGD in the recipient. The co-relation between these two parameters was evaluated. MATERIAL AND METHODS In this pilot study, we prospectively collected blood samples and lung biopsies in thirteen donors at the time of recovery of organs before preservation. Gene expression of SP-A, SP-B, SP-D, and CC16 was evaluated by real-time quantitative PCR...
June 16, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28619132/co-infection-of-h9n2-subtype-avian-influenza-virus-and-infectious-bronchitis-virus-decreases-sp-a-expression-level-in-chickens
#9
Qi Huang, Kai Wang, Ling Pan, Kezong Qi, Hongmei Liu, Hongjun Chen
Chicken surfactant protein A (cSP-A) is a collectin believed to play an important role in antiviral immunity. However, cSP-A expression in the respiratory tract of chickens after viral co-infection remains unclear. The aim of this study was the detection and characterization of cSP-A in co-infected chickens. For this purpose, four-week-old specific pathogen-free (SPF) chickens were divided into five groups and inoculated intranasally with H9N2 subtype avian influenza virus (AIV), infectious bronchitis virus (IBV), or Newcastle disease virus (NDV)...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28617608/anti-ice-nucleating-activity-of-surfactants-against-silver-iodide-in-water-in-oil-emulsions
#10
Takaaki Inada, Toshie Koyama, Hiroyuki Tomita, Takuya Fuse, Chikako Kuwabara, Keita Arakawa, Seizo Fujikawa
Various water-soluble substances are known as anti-ice nucleating agents (anti-INAs), which inhibit heterogeneous ice nucleation initiated by ice nucleating agents (INAs). Among them, several surfactants are reportedly effective as anti-INAs especially against silver iodide (AgI), which is a typical inorganic INA that induces heterogeneous ice nucleation at relatively high temperatures. In this study, the anti-ice nucleating activities of seven surfactants were examined in emulsified surfactant solutions containing AgI particles...
June 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28615099/-hyperoxia-modulates-the-expressions-of-c-ebp%C3%AE-and-pulmonary-surfactant-proteins-in-aecii-of-premature-rats
#11
Yanmin Lu, Hongyan Lu, Jiangyan Liu, Ming Chang, Qiuxia Wang, Wei Tang
Objective To explore the effect of hyperoxia on the expressions of CCAAT enhancer binding protein α (C/EBPα) and pulmonary surfactant proteins (SP-A, SP-B, SP-C, SP-D) and their correlations in primary type II alveolar epithelial cells (AECIIs) from premature rats. Methods AECIIs were divided into an air control group and a hyperoxia model group. The cells of the two groups were respectively exposed toair and 950 mL/L O2. The cells were harvested at 24, 48, and 72 hours after exposure. Inverted phase-contrast microscope was used to observe morphological changes of the cells...
June 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28613935/obstructive-sleep-apnea-and-subclinical-interstitial-lung-disease-in-mesa
#12
John S Kim, Anna J Podolanczuk, Priya Borker, Steven M Kawut, Ganesh Raghu, Joel D Kaufman, Karen D Hinckley Stukovsky, Eric A Hoffman, R Graham Barr, Daniel J Gottlieb, Susan S Redline, David J Lederer
RATIONALE: Obstructive sleep apnea (OSA) has been postulated to contribute to idiopathic pulmonary fibrosis by promoting alveolar epithelial injury via tractional forces and intermittent hypoxia. OBJECTIVE: To determine whether OSA is associated with subclinical interstitial lung disease (ILD) and with biomarkers of alveolar epithelial injury and remodeling. METHODS: We performed cross-sectional analyses of 1,690 community-dwelling adults who underwent 15-channel in-home polysomnography and thoracic computed tomography (CT) imaging in the Multi-Ethnic Study of Atherosclerosis...
June 14, 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/28610627/multiple-biomarkers-predict-disease-severity-progression-and-mortality-in-copd
#13
Rachel L Zemans, Sean Jacobson, Jason Keene, Katerina Kechris, Bruce E Miller, Ruth Tal-Singer, Russell P Bowler
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease characterized by multiple subtypes and variable disease progression. Blood biomarkers have been variably associated with subtype, severity, and disease progression. Just as combined clinical variables are more highly predictive of outcomes than individual clinical variables, we hypothesized that multiple biomarkers may be more informative than individual biomarkers to predict subtypes, disease severity, disease progression, and mortality...
June 13, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28603019/friability-testing-as-a-new-stress-stability-assay-for-biopharmaceuticals
#14
Tetsuo Torisu, Takahiro Maruno, Saki Yoneda, Yoshinori Hamaji, Shinya Honda, Tadayasu Ohkubo, Susumu Uchiyama
A cycle of dropping and shaking a vial containing antibody solution was reported to induce aggregation. In this study, antibody solutions in glass prefillable syringes with or without silicone oil lubrication were subjected to the combined stresses of dropping and shaking, using a friability testing apparatus. Larger numbers of subvisible particles were generated, regardless of silicone oil lubrication, upon combination stress exposure than that with shaking stress alone. Nucleation of antibody molecules upon perturbation by an impact of the dropping, and/or adsorption of antibody molecules to the syringe surface followed by film formation and antibody film desorption were considered key steps in the particle formation promoted by combination stress...
June 8, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28601054/association-of-serum-levels-of-pentraxin-3-m-ficolin-and-surfactant-protein-a-with-the-severity-of-ischemic-stroke
#15
Ebrahim Kouchaki, Atousa Babamohammadi, Hassan Nikoueinejad, Mojtaba Sehat
Stroke is one of the most leading causes of death and disability in the world. Complement system activation contributes to pathogenesis and neuronal damage following stroke. There are no defined biological serum markers to determine the severity of stroke in acute phases. The purpose of current study was to determine the association of three complement activators, namely Pentraxin-3 (PTX3),M-ficolin, and Surfactant protein A (SPA) with the severity of ischemic stroke. This cross-sectional study was done on 82 patients diagnosed with ischemic stroke at 24-96 hours of initiation of the clinical symptoms during 2014-2015...
April 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/28593401/external-validation-of-a-biomarker-and-clinical-prediction-model-for-hospital-mortality-in-acute-respiratory-distress-syndrome
#16
Zhiguo Zhao, Nancy Wickersham, Kirsten N Kangelaris, Addison K May, Gordon R Bernard, Michael A Matthay, Carolyn S Calfee, Tatsuki Koyama, Lorraine B Ware
PURPOSE: Mortality prediction in ARDS is important for prognostication and risk stratification. However, no prediction models have been independently validated. A combination of two biomarkers with age and APACHE III was superior in predicting mortality in the NHLBI ARDSNet ALVEOLI trial. We validated this prediction tool in two clinical trials and an observational cohort. METHODS: The validation cohorts included 849 patients from the NHLBI ARDSNet Fluid and Catheter Treatment Trial (FACTT), 144 patients from a clinical trial of sivelestat for ARDS (STRIVE), and 545 ARDS patients from the VALID observational cohort study...
June 7, 2017: Intensive Care Medicine
https://www.readbyqxmd.com/read/28592015/-clinical-manifestations-of-three-cases-of-surfactant-protein-c-p-v39l-mutation
#17
J H Chen, D Y Zhao, S H An, Y J Zheng, H P Wang, H L Ma
Objective: To investigate the clinical manifestations of surfactant protein C gene (SFTPC) exon-2 c. 115G>G/T (p.V39L). Method: Patients were screened for the entire coding sequence of SFTPC. Three cases from three children's hospital with mutation in p. V39L were reported. Result: All the three cases were females. The age of onset ranged from 2 months to 7 years. Two cases had recurrent lower respiratory tract infection and failed to thrive. One had chronic anoxia and clubbing fingers. Chest computed tomography (CT) showed diffused ground glass pattern, localized emphysema and intralobular septal thickening...
June 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28591049/impact-of-serum-sp-a-and-sp-d-levels-on-comparison-and-prognosis-of-idiopathic-pulmonary-fibrosis-a-systematic-review-and-meta-analysis
#18
Kai Wang, Qing Ju, Jing Cao, Wenze Tang, Jian Zhang
BACKGROUND AND OBJECTIVE: Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. METHODS: Relevant articles were searched in PubMed, Embase, and Chinese National Knowledge Infrastructure databases and reviewed by 2 independent readers...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28591009/surfactant-proteins-a-and-d-attenuate-lps-induced-apoptosis-in-primary-intestinal-epithelial-cells-iecs
#19
Linlin Zhang, Qinghe Meng, Natesh Yepuri, Guirong Wang, Xiuming Xi, Robert N Cooney
INTRODUCTION: SP-A/D KO mice with sepsis demonstrate more severe lung, kidney and gut injury/apoptosis than WT controls. We hypothesize SP-A and SP-D directly regulate LPS-induced P38 MAPK activation and gut apoptosis during sepsis. METHODS: Primary IECs were established from SP-A/D KO or C57BL/6 WT mice, stimulated with LPS and harvested at 24 h. IECs from WT mice were treated with SP-A, SP-D or vehicle for 20 h, then LPS for 24 h. Apoptosis, cleaved caspase-3 levels and the ratio of BAX/Bcl-2 were assayed...
June 6, 2017: Shock
https://www.readbyqxmd.com/read/28587394/mir-206-inhibits-fn1-expression-and-proliferation-and-promotes-apoptosis-of-rat-type-ii-alveolar-epithelial-cells
#20
Jun Duan, Xiaoying Zhang, Sheng Zhang, Shaodong Hua, Zhichun Feng
Bronchopulmonary dysplasia (BPD) is a syndrome of respiratory distress caused by chronic lung injury, primarily in preterm infants. miR-206 and fibronectin 1 (FN1) are associated with the development of BPD. The present study used rat type II alveolar epithelial cells (AECII) to investigate the underlying mechanisms of BPD. AECII were isolated using a primary cell culture prior to alkaline phosphatase staining and immunofluorescence of surfactant protein C (SP-C). These were used to verify the presence of AECII...
June 2017: Experimental and Therapeutic Medicine
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