keyword
https://read.qxmd.com/read/38534618/ph-responsive-cellulose-silk-fe-3-o-4-hydrogel-microbeads-designed-for-biomedical-applications
#1
JOURNAL ARTICLE
Seung Hyeon Weon, Yuhyeon Na, Jiwoo Han, Jeong Woo Lee, Hyung Joo Kim, Saerom Park, Sang Hyun Lee
In this study, cellulose/Fe3 O4 hydrogel microbeads were prepared through the sol-gel transition of a solvent-in-oil emulsion using various cellulose-dissolving solvents and soybean oil without surfactants. Particularly, 40% tetrabutylammonium hydroxide (TBAH) and 40% tetrabutylphosphonium hydroxide (TBPH) dissolved cellulose at room temperature and effectively dispersed Fe3 O4 , forming cellulose/Fe3 O4 microbeads with an average diameter of ~15 µm. Additionally, these solvents co-dissolved cellulose and silk, allowing for the manufacture of cellulose/silk/Fe3 O4 hydrogel microbeads with altered surface characteristics...
March 16, 2024: Gels
https://read.qxmd.com/read/38530420/selective-hydrolysis-of-ovalbumin-by-zr-based-lacunary-polyoxotungstate-in-surfactant-solutions
#2
JOURNAL ARTICLE
Malihe Babaei Zarch, Maryam Bazargan, Masoud Mirzaei
This study aims to design an artificial metalloprotease based on a Zr-containing polyoxometalate Na8 [Zr(W5 O18 )2 ] [Zr(W5 )2 ] for the hydrolysis of ovalbumin (OVA) in the presence of different surfactants, which can be used in many areas of the biological and medical sciences, particularly for targeted proteolytic drug design. For this reason, parameters, including the free energy of binding, the chemical nature of amino acid residues, secondary structures, and electrostatic potentials, of Zr(W5 )2 -OVA and Zr(W5 )2 -OVA-surfactant were analyzed by molecular docking simulations...
March 26, 2024: Inorganic Chemistry
https://read.qxmd.com/read/38526635/characterizing-interregional-differences-in-the-rheological-properties-and-composition-of-rat-small-intestinal-mucus
#3
JOURNAL ARTICLE
Mette Klitgaard, Jette Jacobsen, Maja Nørgaard Kristensen, Ragna Berthelsen, Anette Müllertz
The mucus layer in the small intestine is generally regarded as a barrier to drug absorption. However, the mucus layer is a complex system, and presently, only a few studies have been conducted to elucidate its physicochemical properties. The current study hypothesizes that the mucus layer contains solubility-enhancing surfactants and thus might aid the oral absorption of poorly water-soluble drugs. Mucus was sampled from sections of the small intestine of fasted rats to analyze the rheological properties and determine the mucus pH and concentrations of proteins and endogenous surfactants, i...
March 25, 2024: Drug Delivery and Translational Research
https://read.qxmd.com/read/38521812/diatom-inspired-silicification-process-for-development-of-green-flexible-silica-composite-aerogels
#4
JOURNAL ARTICLE
Valerie Tan, Florian Berg, Hajar Maleki
In this study, we have developed novel biomimetic silica composite aerogels and cryogels for the first time, drawing inspiration from the natural diatom's silicification process. Our biomimetic approach involved the modification of tyrosinase-mediated oxidized silk fibroin (SFO) surfaces with polyethyleneimine (PEI). This modification introduced ample amine groups onto the SF polymer, which catalyzed the silicification of the SFO-PEI gel surface with silicic acid. This process emulates the catalytic function of long-chain polyamines and silaffin proteins found in diatoms, resulting in a silica network structure on the primary SFO-PEI network gel's surface...
March 23, 2024: Scientific Reports
https://read.qxmd.com/read/38519813/identification-of-surfactant-impact-on-a-monoclonal-antibody-characterization-via-hplc-separation-based-and-biophysical-methods
#5
JOURNAL ARTICLE
Kaizhu Guo, Jing Song, Petra Bennington, Alexander J Pavon, Jameson R Bothe, Hanmi Xi, Rico C Gunawan
PURPOSE OR OBJECTIVE: Surfactants, including polysorbates and poloxamers, play a crucial role in the formulation of therapeutic proteins by acting as solubilizing and stabilizing agents. They help prevent protein aggregation and adsorption, thereby enhancing the stability of drug substance and products., However, it is important to note that utilizing high concentrations of surfactants in protein formulations can present significant analytical challenges, which can ultimately affect the product characterization...
March 22, 2024: Pharmaceutical Research
https://read.qxmd.com/read/38518719/novel-air-liquid-interface-culture-model-to-investigate-stiffness-dependent-behaviors-of-alveolar-epithelial-cells
#6
JOURNAL ARTICLE
Yuto Takahashi, Satoru Ito, Jungfeng Wang, Jeonghyun Kim, Takeo Matsumoto, Eijiro Maeda
Pulmonary alveoli are functional units in gas exchange in the lung, and their dysfunctions in lung diseases such as interstitial pneumonia are accompanied by fibrotic changes in structure, elevating the stiffness of extracellular matrix components. The present study aimed to test the hypothesis that such changes in alveoli stiffness induce functional alteration of epithelial cell functions, exacerbating lung diseases. For this, we have developed a novel method of culturing alveolar epithelial cells on polyacrylamide gel with different elastic modulus at an air-liquid interface...
March 15, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38516618/theoretical-framework-and-experimental-solution-for-the-air-water-interface-adsorption-problem-in-cryoem
#7
JOURNAL ARTICLE
Joon S Kang, Xueting Zhou, Yun-Tao Liu, Kaituo Wang, Z Hong Zhou
As cryogenic electron microscopy (cryoEM) gains traction in the structural biology community as a method of choice for determining atomic structures of biological complexes, it has been increasingly recognized that many complexes that behave well under conventional negative-stain electron microscopy tend to have preferential orientation, aggregate or simply mysteriously "disappear" on cryoEM grids. However, the reasons for such misbehavior are not well understood, which limits systematic approaches to solving the problem...
August 31, 2023: Biophysics Reports
https://read.qxmd.com/read/38513817/how-spray-drying-processing-and-solution-composition-can-affect-the-mabs-stability-in-reconstituted-solutions-for-subcutaneous-injections-part-ii-exploring-each-protein-stabilizer-effect
#8
JOURNAL ARTICLE
Carla M Barcelo, Vasco Filipe, Mostafa Nakach, Maria Inês Ré
Despite extensive research in spray drying of biopharmaceuticals, identifying the optimal formulation composition and process conditions to minimize the various stresses a biopharmaceutical undergoes during this drying process. The current study extends previous research on investigating how spray drying processing and solution composition can affect the stability of monoclonal antibodies (mAbs) in reconstituted solutions for subcutaneous injections. The decoupling process stresses on a model mAb (mAb-A) compared to the effect of coupled spray-drying stresses revealed that excipients and protein concentration had a more pronounced effect on stabilizing mAb-A against shear and thermal/dehydration stresses than spray drying operating conditions...
March 19, 2024: International Journal of Pharmaceutics
https://read.qxmd.com/read/38506718/-in-vitro-and-in-silico-assessment-of-anti-biofilm-and-anti-quorum-sensing-properties-of-2-4-di-tert-butylphenol-against-acinetobacter-baumannii
#9
JOURNAL ARTICLE
Nisha Kumari Jha, Venkadesaperumal Gopu, Chandran Sivasankar, Satya Ranjan Singh, Palanisamy Bruntha Devi, Ayaluru Murali, Prathapkumar Halady Shetty
Introduction. Acinetobacter baumannii is a nosocomial pathogen with a high potential to cause food-borne infections. It is designated as a critical pathogen by the World Health Organization due to its multi-drug resistance and mortalities reported. Biofilm governs major virulence factors, which promotes drug resistance in A. baumannii . Thus, a compound with minimum selection pressure on the pathogen can be helpful to breach biofilm-related virulence. Hypothesis/Gap Statement. To identify anti-biofilm and anti-virulent metabolites from extracts of wild Mangifera indica (mango) brine pickle bacteria that diminishes pathogenesis and resistance of A...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38503212/perfluorooctanoate-and-nano-titanium-dioxide-impair-the-byssus-performance-of-the-mussel-mytilus-coruscus
#10
JOURNAL ARTICLE
Bingyan Sun, Yueyong Shang, Haodong Chen, Kiran Khadka, Yiting Pan, Menghong Hu, Youji Wang
Perfluorooctanoate (PFOA) is widely used as a surfactant and has metabolic, immunologic, developmental, and genetic toxicity on marine organisms. However, the effects of PFOA on individual defense functions in mussels in the presence of titanium dioxide nanoparticles (nano-TiO2 ) are poorly understood. To investigate the defense strategies and regulatory mechanisms of mussels under combined stressors, the thick-shell mussels Mytilus coruscus were exposed to different PFOA concentrations (0, 2 and 200 μg/L) and nano-TiO2 (0 and 0...
March 18, 2024: Journal of Hazardous Materials
https://read.qxmd.com/read/38502024/anion-competition-at-positively-charged-surfactant-monolayers
#11
JOURNAL ARTICLE
Laura Mortara, Tetiana Mukhina, Hernan Chaimovich, Gerald Brezesinski, Nico F A van der Vegt, Emanuel Schneck
Interactions of anions with hydrophobic surfaces of proteins and water-soluble polymers depend on the ability of the ions to shed their hydration shells. At positively charged surfactant monolayers, the interactions of anions are less well understood. Due to the interplay of electrostatic surface forces, hydration effects, and ion-ion interactions in the electrostatic double layer, a comprehensive microscopic picture remains elusive. Herein, we study the interactions of chloride, bromide, and a mixture of these two anions at the aqueous interface of dihexadecyldimethylammonium (DHDA+ ) and dioctadecyldimethylammonium (DODA+ ) cationic monolayers...
March 19, 2024: Langmuir: the ACS Journal of Surfaces and Colloids
https://read.qxmd.com/read/38501650/polymyxin-b-enriched-exogenous-lung-surfactant-thermodynamics-and-structure
#12
JOURNAL ARTICLE
Nina Královič-Kanjaková, Ali Asi Shirazi, Lukáš Hubčík, Mária Klacsová, Atoosa Keshavarzi, Juan Carlos Martínez, Sophie Combet, José Teixeira, Daniela Uhríková
The use of an exogenous pulmonary surfactant (EPS) to deliver other relevant drugs to the lungs is a promising strategy for combined therapy. We evaluated the interaction of polymyxin B (PxB) with a clinically used EPS, the poractant alfa Curosurf (PSUR). The effect of PxB on the protein-free model system (MS) composed of four phospholipids (diC16:0PC/16:0-18:1PC/16:0-18:2PC/16:0-18:1PG) was examined in parallel to distinguish the specificity of the composition of PSUR. We used several experimental techniques (differential scanning calorimetry, small- and wide-angle X-ray scattering, small-angle neutron scattering, fluorescence spectroscopy, and electrophoretic light scattering) to characterize the binding of PxB to both EPS...
March 19, 2024: Langmuir: the ACS Journal of Surfaces and Colloids
https://read.qxmd.com/read/38500106/lung-tissue-expression-of-epithelial-injury-markers-is-associated-with-acute-lung-injury-severity-but-does-not-discriminate-sepsis-from-ards
#13
JOURNAL ARTICLE
Natália de Souza Xavier Costa, Giovana da Costa Sigrist, Alexandre Santos Schalch, Luciano Belotti, Marisa Dolhnikoff, Luiz Fernando Ferraz da Silva
BACKGROUND: Acute respiratory distress syndrome (ARDS) is a common cause of respiratory failure in critically ill patients, and diffuse alveolar damage (DAD) is considered its histological hallmark. Sepsis is one of the most common aetiology of ARDS with the highest case-fatality rate. Identifying ARDS patients and differentiate them from other causes of acute respiratory failure remains a challenge. To address this, many studies have focused on identifying biomarkers that can help assess lung epithelial injury...
March 18, 2024: Respiratory Research
https://read.qxmd.com/read/38500038/development-of-an-in-vitro-human-alveolar-epithelial-air-liquid-interface-model-using-a-small-molecule-inhibitor-cocktail
#14
JOURNAL ARTICLE
Ikuya Tanabe, Kanae Ishimori, Shinkichi Ishikawa
BACKGROUND: The alveolar epithelium is exposed to numerous stimuli, such as chemicals, viruses, and bacteria that cause a variety of pulmonary diseases through inhalation. Alveolar epithelial cells (AECs) cultured in vitro are a valuable tool for studying the impacts of these stimuli and developing therapies for associated diseases. However, maintaining the proliferative capacity of AECs in vitro is challenging. In this study, we used a cocktail of three small molecule inhibitors to cultivate AECs: Y-27632, A-83-01, and CHIR99021 (YAC)...
March 18, 2024: BMC molecular and cell biology
https://read.qxmd.com/read/38497242/combination-drug-therapy-by-herbal-nanomedicine-prevent-multidrug-resistance-protein-1-promote-apoptosis-in-lung-carcinoma
#15
JOURNAL ARTICLE
Ashraf Alemi, Mojtaba Haghi Karamallah, Mohamad Sabaghan, Seyed Ahmad Hosseini, Ali Veisi, Somayeh Haghi Karamallah, Mohammad Farokhifar
Given the numerous adverse effects of lung cancer treatment, more research on non-toxic medications is urgently needed. Curcumin (CUR) and berberine (BBR) combat drug resistance by controlling the expression of multidrug resistant pump (MDR1). Fascinatingly, combining these medications increases the effectiveness of preventing lung cancer. Their low solubility and poor stability, however, restrict their therapeutic efficacy. Because of the improved bioavailability and increased encapsulation effectiveness of water-insoluble medicines, surfactant-based nanovesicles have recently received a great deal of attention...
2024: Journal of Applied Biomaterials & Functional Materials
https://read.qxmd.com/read/38491484/irf4-mediated-treg-phenotype-switching-can-aggravate-hyperoxia-induced-alveolar-epithelial-cell-injury
#16
JOURNAL ARTICLE
He Langyue, Zhu Ying, Jiang Jianfeng, Zhu Yue, Yao Huici, Lu Hongyan
Bronchopulmonary dysplasia (BPD) is characterized by alveolar dysplasia, and evidence indicates that interferon regulatory factor 4 (IRF4) is involved in the pathogenesis of various inflammatory lung diseases. Nonetheless, the significance and mechanism of IRF4 in BPD remain unelucidated. Consequently, we established a mouse model of BPD through hyperoxia exposure, and ELISA was employed to measure interleukin-17 A (IL-17 A) and interleukin-6 (IL-6) expression levels in lung tissues. Western blotting was adopted to determine the expression of IRF4, surfactant protein C (SP-C), and podoplanin (T1α) in lung tissues...
March 15, 2024: BMC Pulmonary Medicine
https://read.qxmd.com/read/38483982/the-expression-of-the-surfactant-proteins-sp-a-and-sp-b-during-postnatal-alveolarization-of-the-rat-lung
#17
JOURNAL ARTICLE
Franziska Roeder, Lars Knudsen, Andreas Schmiedl
OBJECTIVE: Surfactant-specific proteins (SP) are responsible for the functional and structural integrity as well as for the stabilization of the intra-alveolar surfactant. Morphological lung maturation starts in rat lungs after birth. The aim of this study was to investigate whether the expression of the hydrophilic SP-A and the hydrophobic SP-B is associated with characteristic postnatal changes characterizing morphological lung maturation. METHODS: Stereological methods were performed on the light microscope...
2024: PloS One
https://read.qxmd.com/read/38479075/application-of-thermomyces-lanuginosus-polygalacturonase-produced-in-komagataella-phaffii-in-biomass-hydrolysis-and-textile-bioscouring
#18
JOURNAL ARTICLE
Luana Assis Serra, Thais Demarchi Mendes, Janice Lisboa De Marco, João Ricardo Moreira de Almeida
In this work, the polygalacturonase (TL-PG1) from the thermophilic fungus Thermomyces lanuginosus was heterologously produced for the first time in the yeast Komagataella phaffii. The TL-PG1 was successfully expressed under the control of the AOX1 promoter and sequentially purified by His-tag affinity. The purified recombinant pectinase exhibited an activity of 462.6 U/mL toward polygalacturonic acid under optimal conditions (pH 6 and 55 ˚C) with a 2.83 mg/mL and 0.063 μmol/minute for Km and Vmax , respectively...
March 2, 2024: Enzyme and Microbial Technology
https://read.qxmd.com/read/38477414/expression-and-functional-analysis-of-a-recombinant-aquaporin-z-from-antarctic-pseudomonas-sp-ams3
#19
JOURNAL ARTICLE
S Balakrishnan, R N Z R A Rahman, N D M Noor, W Latip, M S M Ali
Aquaporin (AQP) is a water channel protein from the family of transmembrane proteins which facilitates the movement of water across the cell membrane. It is ubiquitous in nature, however the understanding of the water transport mechanism, especially for AQPs in microbes adapted to low temperatures, remains limited. AQP also has been recognized for its ability to be used for water filtration, but knowledge of the biochemical features necessary for its potential applications in industrial processes has been lacking...
March 13, 2024: Proteins
https://read.qxmd.com/read/38463638/inflammation-endothelial-injury-and-the-acute-respiratory-distress-syndrome-after-out-of-hospital-cardiac-arrest
#20
JOURNAL ARTICLE
Sarah C Katsandres, Jane Hall, Kyle Danielson, Sana Sakr, Sarah G Dean, David J Carlbom, Mark M Wurfel, Pavan K Bhatraju, Joseph A Hippensteel, Eric P Schmidt, Kaori Oshima, Catherine R Counts, Michael R Sayre, Daniel J Henning, Nicholas J Johnson
BACKGROUND: Acute respiratory distress syndrome (ARDS) is often seen in patients resuscitated from out-of-hospital cardiac arrest (OHCA). We aim to test whether inflammatory or endothelial injury markers are associated with the development of ARDS in patients hospitalized after OHCA. METHODS: We conducted a prospective, cohort, pilot study at an urban academic medical center in 2019 that included a convenience sample of adults with non-traumatic OHCA. Blood and pulmonary edema fluid (PEF) were collected within 12 hours of hospital arrival...
March 2024: Resuscitation plus
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