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Granulosa theca cell

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https://www.readbyqxmd.com/read/28432272/establishment-of-an-in-vitro-culture-model-of-theca-cells-from-hierarchical-follicles-in-ducks
#1
Xiang Gan, da Chen, Yan Deng, Junsong Yuan, Bo Kang, Jiamin Qiu, Wenqiang Sun, Chunchun Han, Jiwei Hu, Liang Li, Jiwen Wang
Theca cells, including theca interna cells and theca externa cells, are vital components of ovarian follicles. The aim of this study is to identify a reliable method for the in vitro culture of theca cells from duck ovarian hierarchical (F4-F2) follicles. We improved the method for cell separation by using trypsin to further remove granular cells, and we increased the concentration of fetal bovine serum used in in vitro culture to improve cytoactivity. Cell antibody immunofluorescence showed that all inoculated cells could be stained by the CYP17A1/19A1 antibody but not by the FSHR antibody, which could stain granulosa cells...
April 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28409689/effects-of-methoxychlor-on-igf-i-signaling-pathway-in-rat-ovary
#2
O Ozden-Akkaya, K Altunbas, A Yagcı
Follicular development and other ovarian functions are regulated by growth factors that can be affected by exogenous agents. Methoxychlor (MXC) is an organochloride pesticide that causes female infertility. We investigated how MXC affects the distribution of developing ovarian follicles in adult rats after treatment between embryonic day (E) 18 and postnatal day (PND) 7. We also measured insulin-like growth factor-I (IGF-I) and its receptor, IGF-IR, expressions in ovarian follicles and investigated whether MXC changed the levels of IGF-I and IGF-IR in the ovary...
2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28400072/notch-signaling-pathway-promotes-the-development-of-ovine-ovarian-follicular-granulosa-cells
#3
Jiongjie Jing, Xiaolong Jiang, Jianwei Chen, Xiaolei Yao, Miaomiao Zhao, Pengfei Li, Yangyang Pan, Youshe Ren, Wenzhong Liu, Lihua Lyu
The Notch signaling pathway regulates cell proliferation, differentiation and apoptosis involved in development of the organs and tissues such as nervous system, cartilage, lungs, kidneys and prostate as well as the ovarian follicles. This study aimed to investigate the mRNA expression and localization of NOTCH2, as the key factor in Notch signaling pathway. This was determined by PCR, real-time PCR and immunohistochemistry. Additionally, the effects of inhibiting Notch signaling pathway with different concentrations (5μM, 10μM and 20μM) of N-[N-(3, 5-Difuorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT), an inhibitor of Notch signaling pathway, on ovine granulosa cells was determined in vitro by detecting estradiol production using enzyme linked immunosorbent assay and expressions of the genes related to the cell cycle and apoptosis using real-time polymerase chain reaction (PCR)...
March 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28380519/evidence-that-gene-expression-of-ovarian-follicular-tight-junction-proteins-is-regulated-in-vivo-and-in-vitro-in-cattle
#4
L Zhang, L F Schütz, C L Robinson, M L Totty, L J Spicer
Tight junctions (TJ) are common paracellular sealing structures that control the transport of water, ions, and macromolecules across cell layers. Because the role of TJ in bovine follicular development is unknown, we investigated the developmental and hormonal regulation of the transmembrane TJ protein, occludin (OCLN), and the cytoplasmic TJ proteins, TJ protein 1 (TJP1) and cingulin (CGN) in bovine granulosa cells (GC) and theca cells (TC). For this purpose, bovine GC and TC were isolated from large (>8 mm) and/or small (1 to 5 mm) follicles and either extracted for real-time PCR (qPCR) or cultured in vitro...
March 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28347743/transcription-of-cyp19a1-is-directly-regulated-by-sf-1-in-the-theca-cells-of-ovary-follicles-in-chicken
#5
Jing Wang, Yanzhang Gong
Many studies have suggested the important role of estrogen in ovarian differentiation and development of vertebrates including chicken. Cytochrome P450 aromatase, encoded by CYP19A1, is a key enzyme in estrogen synthesis, but the mechanism of CYP19A1 regulation in chicken remains unknown. Here, we found that CYP19A1 was only expressed in the theca cell layers of chicken ovary follicles. Steroidogenic factor 1 (SF-1, also named as nuclear receptor subfamily 5 group A member 1, NR5A1), a potential regulators, was expressed in both the theca cell layers and granulosa cell layers...
March 24, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28345450/erratum
#6
(no author information available yet)
Weber, K. (2017). Differences in Types and Incidence of Neoplasms in Wistar Han and Sprague-Dawley Rats. J Toxicol Pathol45, 64-75. (Original DOI: 10.1177/0192623316672075) In the January 2017 issue of Toxicologic Pathology, a number of in text Table citations were incorrectly labeled. The following are the sentences with corrected Table number (this has also been corrected in the online version): Many fewer neoplasms are recorded for the endocrine pancreas compared with islet cell tumor incidences (Table 3)...
January 1, 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/28341393/cell-specific-immuno-localization-of-progesterone-receptor-alpha-in-the-rabbit-ovary-during-pregnancy-and-after-parturition
#7
Mahmoud Abd-Elkareem
Progesterone receptor alpha (PRA) has a central coordinator role in the ovarian functions in mammals. The aim of this study was to investigate the immunolocalization of PRA in the rabbit ovary during pregnancy and after parturition. The rabbit ovary during pregnancy and after parturition had moderate cytoplasmic and moderate to intense nuclear PRA immunostaining in the ovarian surface epithelial cells, stromal cells and interstitial gland cells. The PRA was also present in granulosa cells and theca interna cells of the growing, small antral and mature Graafian follicles...
March 18, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28319575/foxl2-mutation-status-in-granulosa-theca-cell-tumors-of-the-ovary
#8
Amber Nolan, Nancy M Joseph, Ankur R Sangoi, Joseph Rabban, Charles Zaloudek, Karuna Garg
Ovarian sex-cord stromal tumors that have between 10% and 50% granulosa cells in a prominent fibrothecomatous background have been referred to as granulosa theca cell tumors or mixed granulosa theca cell tumors. The classification and prognosis of these tumors is not clear. Most adult granulosa cell tumors of the ovary harbor a mutation in the FOXL2 gene, whereas fibromas and thecomas lack this mutation. The aim of our study was to assess the FOXL2 mutation status of ovarian granulosa theca cell tumors and to correlate the mutation status with morphologic and clinical characteristics...
March 17, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28315656/expression-of-odc1-spd-spm-and-azin1-in-the-hypothalamus-ovary-and-uterus-during-rat-estrous-cycle
#9
Joseph R D Fernandes, Sammit Jain, Arnab Banerjee
The aim of the present study was to investigate variation in the expression pattern of ornithine decarboxylase (ODC1), spermine (SPM), spermidine (SPD) and antizyme inhibitor (AZIN1) in hypothalamus, ovary and uterus during the estrous cycle of rats. Further, to understand any correlation between polyamines and GnRH I expression in hypothalamus; effect of putrescine treatment on GnRH I expression in hypothalamus and progesterone and estradiol levels in serum were investigated. The study also aims in quantifying all the immunohistochemistry images obtained based on pixel counting algorithm to yield the relative pixel count...
March 16, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28299992/developmental-programming-gestational-exposure-to-excess-testosterone-alters-expression-of-ovarian-matrix-metalloproteases-and-their-target-proteins
#10
Muraly Puttabyatappa, Ashleigh Irwin, Jacob D Martin, Makeda Mesquitta, Almudena Veiga-Lopez, Vasantha Padmanabhan
Prenatal testosterone (T)-treated sheep, similar to women with polycystic ovary syndrome (PCOS), manifests reproductive defects that include multifollicular ovarian phenotype. Women with PCOS manifest increased ovarian matrix metalloproteinases (MMPs) activity. We tested the hypothesis that gestational T excess in sheep would alter ovarian expression of MMPs, tissue inhibitors of MMP (TIMP) and their target proteins laminin B (LAMB), collagen, tumor necrosis factor alpha (TNF), and connexin 43 (GJA1) consistent with increased MMP activity and that these changes are developmentally regulated...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28282394/transcriptomal-profiling-of-bovine-ovarian-granulosa-and-theca-interna-cells-in-primary-culture-in-comparison-with-their-in-vivo-counterparts
#11
Nicholas Hatzirodos, Claire Glister, Katja Hummitzsch, Helen F Irving-Rodgers, Philip G Knight, Raymond J Rodgers
In vitro culture of ovarian granulosa cells and theca cells has been very important for our understanding of their function and regulation. One of the most eagerly sought attributes of cell culture is the use of chemically-defined conditions. However, even under such in vitro conditions cell behaviour could differ from the in vivo situation because of differences in oxygen tension, nutrients, adhesion matrix and other factors. To examine this further we compared the transcriptomes of both granulosa cells and cells from the theca interna that were cultured in what are arguably the best in vitro conditions for maintaining the 'follicular' phenotypes of both tissue types, as displayed by their respective freshly-isolated counterparts...
2017: PloS One
https://www.readbyqxmd.com/read/28250234/apelin-apln-regulates-progesterone-secretion-and-oocyte-maturation-in-bovine-ovarian-cells
#12
Jennifer Roche, Christelle Ramé, Maxime Reverchon, Namya Mellouk, Agnieska Rak, Pascal Froment, Joëlle Dupont
APLN and its G-protein coupled receptor APLNR are expressed in the bovine ovary. However, their role in granulosa cells and oocytes is unknown. Here, we studied their expression in bovine ovarian cells and investigated their regulation in cultured luteinizing granulosa cells in response to IGF1 and FSH. We determined the effect and the molecular mechanism of APLN (isoforms 17 and 13) on bovine granulosa cell progesterone secretion and on oocyte maturation. By RT-qPCR and immunoblot, we showed that the expression of both APLN and APLNR in granulosa and oocytes significantly increased with ovarian follicles size whereas it was similar in theca interstitial cells...
March 1, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28107515/regulatory-mechanisms-underlying-the-expression-of-prolactin-receptor-in-chicken-granulosa-cells
#13
Shenqiang Hu, Raj Duggavathi, David Zadworny
Prolactin (PRL) has both pro- and anti-gonadal roles in the regulation of avian ovarian functions through its interaction with the receptor (PRLR). However, neither the pattern of expression of PRLR nor its regulatory mechanisms during follicle development have been clearly defined. The objective of the present study was to investigate mechanisms of PRLR expression in chicken granulosa cells. Levels of PRLR transcript were highest in the stroma and walls of follicles < 2 mm in diameter and progressively declined with the maturation of follicles...
2017: PloS One
https://www.readbyqxmd.com/read/28078843/association-between-expression-of-cumulus-expansion-markers-and-real-time-proliferation-of-porcine-follicular-granulosa-cells-in-a-primary-cell-culture-model
#14
S Ciesiółka, J Budna, A Bryja, W Kranc, A Chachuła, M Dyszkiewicz-Konwińska, H Piotrowska, D Bukowska, P Antosik, M Bruska, K P Brüssow, M Nowicki, M Zabel, B Kempisty
Folliculogenesis is a compound process that involves both ovarian follicle growth and oocyte development, which is tightly attached to the follicular wall. During this process, cells that form the follicle structure undergo substantial morphological and molecular modifications that finally lead to differentiation and specialization of ovarian follicular cells. The differentiation of ovarian cells encompasses formation of follicle, which is composed of theca (TCs), mural granulosa (GCs), and cumulus cells (CCs)...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28069904/detection-and-activity-of-11-beta-hydroxylase-cyp11b1-in-the-bovine-ovary
#15
Ayelen N Amweg, Fernanda Mariel Rodriguez, Emilia Huber, Belkis E Marelli, Natalia Gareis, Eduardo M Belotti, Florencia Rey, Natalia Raquel Salvetti, Hugo H Ortega
Glucocorticoids (GCs) such as cortisol and corticosterone are important steroid hormones with different functions in intermediate metabolism, development, cell differentiation, immune response and reproduction. In response to physiological and immunological stress, adrenocorticotropic hormone (ACTH) acts on the adrenal gland by stimulating synthesis and secretion of GCs. However, there is increasing evidence that GCs may also be synthesized by extra-adrenal tissues. Here, we examined the gene and protein expression of the enzyme 11β-hydroxylase P450c11 (CYP11B1), involved in the conversion of 11-deoxycortisol to cortisol, in the different components of the bovine ovary, and determined the functionality of CYP11B1 in vitro...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28018460/unusual-malignant-neoplasms-of-ovary-in-children-two-cases-report
#16
Ali Ghribi, Aicha Bouden, Manef Gasmi, Mourad Hamzaoui
Sex cord tumors with annular tubules are known to originate from the sex cord of embryonic gonads that synthesize Sertoli cells, Leydig cells, granulosa cells, and theca cells of the ovarian stroma, while ovarian small cell carcinoma of the hypercalcemic type is a type of neuroendocrine tumor. Both these tumors are uncommon, potentially malignant neoplasms in children. We report the case of a sex cord tumor with annular tubules in an 11-year-old girl and a case of small cell carcinoma of the hypercalcemic type in a 10-year-old girl...
November 2016: Korean Journal of Pediatrics
https://www.readbyqxmd.com/read/28004024/transcriptomes-of-bovine-ovarian-follicular-and-luteal-cells
#17
Sarah M Romereim, Adam F Summers, William E Pohlmeier, Pan Zhang, Xiaoying Hou, Heather A Talbott, Robert A Cushman, Jennifer R Wood, John S Davis, Andrea S Cupp
Affymetrix Bovine GeneChip® Gene 1.0 ST Array RNA expression analysis was performed on four somatic ovarian cell types: the granulosa cells (GCs) and theca cells (TCs) of the dominant follicle and the large luteal cells (LLCs) and small luteal cells (SLCs) of the corpus luteum. The normalized linear microarray data was deposited to the NCBI GEO repository (GSE83524). Subsequent ANOVA determined genes that were enriched (≥2 fold more) or decreased (≤-2 fold less) in one cell type compared to all three other cell types, and these analyzed and filtered datasets are presented as tables...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/27917511/evidence-for-direct-effects-of-glyphosate-on-ovarian-function-glyphosate-influences-steroidogenesis-and-proliferation-of-bovine-granulosa-but-not-theca-cells-in-vitro
#18
Maria Chiara Perego, Luis F Schutz, Francesca Caloni, Cristina Cortinovis, Marco Albonico, Leon J Spicer
Glyphosate (GLY) is a common herbicide used worldwide but its effect on ovarian function in mammals is unknown. The aim of this study was to determine the potential endocrine disruptor effects of GLY on ovarian function evaluating cell proliferation, steroidogenesis and gene expression using bovine granulosa cells (GC) and theca cells as in vitro models. GC proliferation was impaired (P < 0.05) after exposure to GLY at 0.5, 1.7 and 5 μg ml(-1) . GC progesterone production was not affected (P ≥ 0...
December 5, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27888191/wt1-directs-the-lineage-specification-of-sertoli-and-granulosa-cells-by-repressing-sf1-expression
#19
Min Chen, Lianjun Zhang, Xiuhong Cui, Xiwen Lin, Yaqiong Li, Yaqing Wang, Yanbo Wang, Yan Qin, Dahua Chen, Chunsheng Han, Bin Zhou, Vicki Huff, Fei Gao
Supporting cells (Sertoli and granulosa) and steroidogenic cells (Leydig and theca-interstitium) are two major somatic cell types in mammalian gonads. However, the mechanisms that control their differentiation during gonad development remain elusive. In this study, we found deletion of Wt1 in ovary after sex determination caused ectopic development of steroidogenic cells at embryonic stage. Furthermore, the differentiation of both Sertoli and granulosa cells was blocked when Wt1 was deleted before sex determination and most genital ridge somatic cells differentiated into steroidogenic cells in both male and female gonads...
November 25, 2016: Development
https://www.readbyqxmd.com/read/27872196/induction-of-ectonucleotide-pyrophosphatase-phosphodiesterase-3-during-the-periovulatory-period-in-the-rat-ovary
#20
Fei-Xue Li, Jiao-Jiao Yu, Ying Liu, Xiao-Ping Miao, Thomas E Curry
Ectonucleotide pyrophosphatase/phosphodiesterase 3 (Enpp3) is involved in multiple physiological processes, such as morphological changes and inflammatory processes. The present study investigated the spatiotemporal expression pattern and regulatory mechanisms controlling expression of Enpp3 in the rat ovary during the periovulatory period. Immature female rats were injected with pregnant mare serum gonadotropin to stimulate follicular development. Ovaries, granulosa cells, or theca-interstitial cells were collected at various times after human chorionic gonadotropin (hCG) administration...
November 20, 2016: Reproductive Sciences
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