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DAGLα

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https://www.readbyqxmd.com/read/28859076/developmental-programming-of-somatic-growth-behavior-and-endocannabinoid-metabolism-by-variation-of-early-postnatal-nutrition-in-a-cross-fostering-mouse-model
#1
Felix Schreiner, Merle Ackermann, Michael Michalik, Eva Hucklenbruch-Rother, Andras Bilkei-Gorzo, Ildiko Racz, Laura Bindila, Beat Lutz, Jörg Dötsch, Andreas Zimmer, Joachim Woelfle
BACKGROUND: Nutrient deprivation during early development has been associated with the predisposition to metabolic disorders in adulthood. Considering its interaction with metabolism, appetite and behavior, the endocannabinoid (eCB) system represents a promising target of developmental programming. METHODS: By cross-fostering and variation of litter size, early postnatal nutrition of CB6F1-hybrid mice was controlled during the lactation period (3, 6, or 10 pups/mother)...
2017: PloS One
https://www.readbyqxmd.com/read/28579484/acylethanolamides-and-endocannabinoid-signaling-system-in-dorsal-striatum-of-rats-exposed-to-perinatal-asphyxia
#2
Mariana I Holubiec, Juan I Romero, Eduardo Blanco, Tamara Logica Tornatore, Juan Suarez, Fernando Rodríguez de Fonseca, Pablo Galeano, Francisco Capani
Endocannabinoids (eCBs) and acylethanolamides (AEs) have lately received more attention due to their neuroprotective functions in neurological disorders. Here we analyze the alterations induced by perinatal asphyxia (PA) in the main metabolic enzymes and receptors of the eCBs/AEs in the dorsal striatum of rats. To induce PA, we used a model developed by Bjelke et al. (1991). Immunohistochemical techniques were carried out to determine the expression of neuronal and glial markers (NeuN and GFAP), eCBs/AEs synthesis and degradation enzymes (DAGLα, NAPE-PLD and FAAH) and their receptors (CB1 and PPARα)...
July 13, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28373843/antihyperalgesic-activities-of-endocannabinoids-in-a-mouse-model-of-antiretroviral-induced-neuropathic-pain
#3
Neha Munawar, Mabayoje A Oriowo, Willias Masocha
Background: Nucleoside reverse transcriptase inhibitors (NRTIs) are the cornerstone of the antiretroviral therapy for human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS). However, their use is sometimes limited by the development of a painful sensory neuropathy, which does not respond well to drugs. Smoked cannabis has been reported in clinical trials to have efficacy in relieving painful HIV-associated sensory neuropathy. Objectives: The aim of this study was to evaluate whether the expression of endocannabinoid system molecules is altered during NRTI-induced painful neuropathy, and also whether endocannabinoids can attenuate NRTI-induced painful neuropathy...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28192193/differential-expression-of-endocannabinoid-system-related-genes-in-the-dorsal-hippocampus-following-expression-and-reinstatement-of-morphine-conditioned-place-preference-in-mice
#4
Wei Li, Cong-Li Zhang, Zheng-Guo Qiu
The endocannabinoid signaling plays a critical role in mediating rewarding effects to morphine. The relative stability for the expression and reinstatement of morphine conditioned place preference (CPP) suggests the involvement of differential neuroadaptations in learned associations between environmental cues and morphine. Changes in gene expression in hippocampus through the endogenous cannabinoid system (eCB) may accompany and mediate the development of such neuroadaptations to repeated morphine stimulation...
March 16, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28147243/song-associated-reward-correlates-with-endocannabinoid-related-gene-expression-in-male-european-starlings-sturnus-vulgaris
#5
Allison H Hahn, Devin P Merullo, Jeremy A Spool, Caroline S Angyal, Sharon A Stevenson, Lauren V Riters
Vocal communication is required for successful social interactions in numerous species. During the breeding season, songbirds produce songs that are reinforced by behavioral consequences (e.g., copulation). However, some songbirds also produce songs not obviously directed at other individuals. The consequences maintaining or reinforcing these songs are less obvious and the neural mechanisms associated with undirected communication are not well-understood. Previous studies indicate that undirected singing is intrinsically rewarding and mediated by opioid or dopaminergic systems; however, endocannabinoids are also involved in regulating reward and singing behavior...
March 27, 2017: Neuroscience
https://www.readbyqxmd.com/read/27999963/identification-of-an-endocannabinoid-system-in-the-rat-pars-tuberalis-a-possible-interface-in-the-hypothalamic-pituitary-adrenal-system
#6
Arsalan Jafarpour, Faramarz Dehghani, Horst-Werner Korf
Endocannabinoids (ECs) are ubiquitous endogenous lipid derivatives and play an important role in intercellular communication either in an autocrine/paracrine or in an endocrine fashion. Recently, an intrinsic EC system has been discovered in the hypophysial pars tuberalis (PT) of hamsters and humans. In hamsters, this EC system is under photoperiodic control and appears to influence the secretion of hormones such as prolactin from the adenohypophysis. We investigate the EC system in the PT of the rat, a frequently used species in endocrine research...
April 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/27623930/important-role-of-endocannabinoid-signaling-in-the-development-of-functional-vision-and-locomotion-in-zebrafish
#7
Andrea Martella, Rosa M Sepe, Cristoforo Silvestri, Jingjing Zang, Giulia Fasano, Oliana Carnevali, Paolo De Girolamo, Stephan C F Neuhauss, Paolo Sordino, Vincenzo Di Marzo
The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report the presence of a complete endocannabinoid system during zebrafish development and show that the genes that code for enzymes that catalyze the anabolism and catabolism (mgll and dagla) of the endocannabinoid, 2-AG (2-arachidonoylglycerol), as well as 2-AG main receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino-induced transient knockdown of the zebrafish Daglα homolog and its pharmacologic rescue, we suggest that synthesis of 2-AG is implicated in the control of axon formation in the midbrain-hindbrain region and that animals that lack Daglα display abnormal physiological behaviors in tests that measure stereotyped movement and motion perception...
December 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27595600/regulated-endosomal-trafficking-of-diacylglycerol-lipase-alpha-dagl%C3%AE-generates-distinct-cellular-pools-implications-for-endocannabinoid-signaling
#8
Ya Zhou, Fiona V Howell, Oleg O Glebov, David Albrecht, Gareth Williams, Patrick Doherty
Diacylglycerol lipase alpha (DAGLα) generates the endocannabinoid (eCB) 2-arachidonylglycerol (2-AG) that regulates the proliferation and differentiation of neural stem cells and serves as a retrograde signaling lipid at synapses. Nothing is known about the dynamics of DAGLα expression in cells and this is important as it will govern where 2-AG can be made and released. We have developed a new construct to label DAGLα at the surface of live cells and follow its trafficking. In hippocampal neurons a cell surface pool of DAGLα co-localizes with Homer, a postsynaptic density marker...
October 2016: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/27578612/sex-differences-in-alcohol-consumption-and-alterations-in-nucleus-accumbens-endocannabinoid-mrna-in-alcohol-dependent-rats
#9
Angela M Henricks, Anthony L Berger, Janelle M Lugo, Lydia N Baxter-Potter, Kennedy V Bieniasz, Rebecca M Craft, Ryan J McLaughlin
Chronic intermittent alcohol (CIA) exposure produces altered motivational states characterized by anxiety and escalated alcohol consumption during withdrawal. The endocannabinoid (ECB) system contributes to these symptoms, and sex differences in alcohol dependence, as well as bidirectional interactions between ECBs and gonadal hormones have been documented. Thus, we evaluated sex differences in alcohol consumption, anxiety-like behavior, and ECB mRNA expression in the nucleus accumbens (NAc) of alcohol-dependent rats during acute withdrawal...
October 29, 2016: Neuroscience
https://www.readbyqxmd.com/read/27327781/the-endocannabinoid-system-in-the-baboon-papio-spp-as-a-complex-framework-for-developmental-pharmacology
#10
Iram P Rodriguez-Sanchez, Josee Guindon, Marco Ruiz, M Elizabeth Tejero, Gene Hubbard, Laura E Martinez-de-Villarreal, Hugo A Barrera-Saldaña, Edward J Dick, Anthony G Comuzzie, Natalia E Schlabritz-Loutsevitch
INTRODUCTION: The consumption of marijuana (exogenous cannabinoid) almost doubled in adults during last decade. Consumption of exogenous cannabinoids interferes with the endogenous cannabinoid (or "endocannabinoid" (eCB)) system (ECS), which comprises N-arachidonylethanolamide (anandamide, AEA), 2-arachidonoyl glycerol (2-AG), endocannabinoid receptors (cannabinoid receptors 1 and 2 (CB1R and CB2R), encoded by CNR1 and CNR2, respectively), and synthesizing/degrading enzymes (FAAH, fatty-acid amide hydrolase; MAGL, monoacylglycerol lipase; DAGL-α, diacylglycerol lipase-alpha)...
November 2016: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/27245901/assay-of-dagl%C3%AE-%C3%AE-activity
#11
Tiziana Bisogno
The endocannabinoid 2-arachidonoylglycerol (2-AG) exerts its physiological action by binding to and functionally activating type-1 (CB1) and type-2 (CB2) cannabinoid receptors. It is thought to be produced through the action of sn-1 selective diacylglycerol lipase (DAGL) that catalyzes 2-AG biosynthesis from sn-2-arachidonate-containing diacylglycerols. Since 2-AG biosynthetic enzymes have been identified only recently, little information on methodological approaches for measuring DAGL activity is as yet available...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27167970/fabp-1-gene-ablation-impacts-brain-endocannabinoid-system-in-male-mice
#12
Gregory G Martin, Sarah Chung, Danilo Landrock, Kerstin K Landrock, Huan Huang, Lawrence J Dangott, Xiaoxue Peng, Martin Kaczocha, Drew R Seeger, Eric J Murphy, Mikhail Y Golovko, Ann B Kier, Friedhelm Schroeder
Liver fatty acid-binding protein (FABP1, L-FABP) has high affinity for and enhances uptake of arachidonic acid (ARA, C20:4, n-6) which, when esterified to phospholipids, is the requisite precursor for synthesis of endocannabinoids (EC) such as arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG). The brain derives most of its ARA from plasma, taking up ARA and transporting it intracellularly via cytosolic fatty acid-binding proteins (FABPs 3,5, and 7) localized within the brain. In contrast, the much more prevalent cytosolic FABP1 is not detectable in the brain but is instead highly expressed in the liver...
August 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27130217/quality-of-ehr-data-extractions-for-studies-of-preterm-birth-in-a-tertiary-care-center-guidelines-for-obtaining-reliable-data
#13
Lindsey A Knake, Monika Ahuja, Erin L McDonald, Kelli K Ryckman, Nancy Weathers, Todd Burstain, John M Dagle, Jeffrey C Murray, Prakash Nadkarni
BACKGROUND: The use of Electronic Health Records (EHR) has increased significantly in the past 15 years. This study compares electronic vs. manual data abstractions from an EHR for accuracy. While the dataset is limited to preterm birth data, our work is generally applicable. We enumerate challenges to reliable extraction, and state guidelines to maximize reliability. METHODS: An Epic™ EHR data extraction of structured data values from 1,772 neonatal records born between the years 2001-2011 was performed...
April 29, 2016: BMC Pediatrics
https://www.readbyqxmd.com/read/27115711/assay-and-inhibition-of-the-purified-catalytic-domain-of-diacylglycerol-lipase-beta
#14
Praveen K Singh, Rachel Markwick, Leanne Lu, Fiona V Howell, Gareth Williams, Patrick Doherty
The diacylglycerol lipases (DAGLα and DAGLβ) hydrolyze DAG to generate 2-arachidonoylglycerol (2-AG), the principal endocannabinoid and main precursor of arachidonic acid (AA). The DAGLs make distinct tissue specific contributions toward 2-AG and AA levels, and therefore, selective modulators for these enzymes could play crucial roles toward harnessing their therapeutic potential. Relatively high-throughput assays have recently been reported for DAGLα and have proven useful toward the characterization of inhibitors of this enzyme...
May 17, 2016: Biochemistry
https://www.readbyqxmd.com/read/27114924/prolonged-activation-of-human-islet-cannabinoid-receptors-in-vitro-induces-adaptation-but-not-dysfunction
#15
Alonso Vilches-Flores, Zara Franklin, Astrid C Hauge-Evans, Bo Liu, Guo C Huang, Pratik Choudhary, Peter M Jones, Shanta J Persaud
BACKGROUND: Although in vivo studies have implicated endocannabinoids in metabolic dysfunction, little is known about direct, chronic activation of the endocannabinoid system (ECS) in human islets. Therefore, this study investigated the effects of prolonged exposure to cannabinoid agonists on human islet gene expression and function. METHODS: Human islets were maintained for 2 and 5 days in the absence or presence of CB1r (ACEA) or CB2r (JWH015) agonists. Gene expression was quantified by RT-PCR, hormone levels by radioimmunoassay and apoptosis by caspase activities...
June 2016: BBA Clinical
https://www.readbyqxmd.com/read/27057735/peripherally-inserted-central-catheters-optimize-nutrient-intake-in-moderately-preterm-infants
#16
Anne L Smazal, Anne B Kavars, Susan J Carlson, Tarah T Colaizy, John M Dagle
BACKGROUND: While very preterm (<32 wk gestation) infants are routinely provided intensive nutritional support via central line, clinical practice varies for nutrient delivery in infants born moderately preterm (32-34 wk gestation). We sought to define the impact of nutritional support via peripherally inserted central catheter (PICC) on nutrient delivery in the first 2 wk of life and growth by discharge. METHODS: Data were extracted from the records of 187 infants born between 32 and 34 6/7 wk gestation and admitted to the University of Iowa Children's Hospital between April 2012 and December 2013...
August 2016: Pediatric Research
https://www.readbyqxmd.com/read/27013337/a-novel-live-cell-assay-to-measure-diacylglycerol-lipase-%C3%AE-activity
#17
Praveen K Singh, Rachel Markwick, Fiona V Howell, Gareth Williams, Patrick Doherty
Diacylglycerol lipase α (DAGLα) hydrolyses DAG to generate the principal endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) in the central nervous system. DAGLα dependent cannabinoid (CB) signalling has been implicated in numerous processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measurement of diacylglycerol lipase (DAGL) activity using its native substrate generally involves low-throughput MS techniques...
June 2016: Bioscience Reports
https://www.readbyqxmd.com/read/26987678/effects-of-the-antipsychotic-paliperidone-on-stress-induced-changes-in-the-endocannabinoid-system-in-rat-prefrontal-cortex
#18
Karina S MacDowell, Aline Sayd, Borja García-Bueno, Javier R Caso, José L M Madrigal, Juan Carlos Leza
Objectives There is a need to explore novel mechanisms of action of existing/new antipsychotics. One potential candidate is the endocannabinoid system (ECS). The present study tried to elucidate the effects of the antipsychotic paliperidone on stress-induced ECS alterations. Methods Wister rats were submitted to acute/chronic restraint stress. Paliperidone (1 mg/kg) was given prior each stress session. Cannabinoid receptors and endocannabinoids (eCBs) synthesis and degradation enzymes were measured in prefrontal cortex (PFC) samples by RT-PCR and Western Blot...
March 18, 2016: World Journal of Biological Psychiatry
https://www.readbyqxmd.com/read/26977322/a-comparative-analysis-of-the-endocannabinoid-system-in-the-retina-of-mice-tree-shrews-and-monkeys
#19
COMPARATIVE STUDY
Joseph Bouskila, Pasha Javadi, Laurent Elkrief, Christian Casanova, Jean-François Bouchard, Maurice Ptito
The endocannabinoid (eCB) system is widely expressed in various parts of the central nervous system, including the retina. The localization of the key eCB receptors, particularly CB1R and CB2R, has been recently reported in rodent and primate retinas with striking interspecies differences. Little is known about the distribution of the enzymes involved in the synthesis and degradation of these eCBs. We therefore examined the expression and localization of the main components of the eCB system in the retina of mice, tree shrews, and monkeys...
2016: Neural Plasticity
https://www.readbyqxmd.com/read/26917221/structure-activity-relationship-studies-on-chemically-non-reactive-glycine-sulfonamide-inhibitors-of-diacylglycerol-lipase
#20
Louis S Chupak, Xiaofan Zheng, Shuanghua Hu, Yazhong Huang, Min Ding, Martin A Lewis, Ryan S Westphal, Yuval Blat, Andrea McClure, Robert G Gentles
N-Benzylic-substituted glycine sulfonamides that reversibly inhibit diacylglycerol (DAG) lipases are reported. Detailed herein are the structure activity relationships, profiling characteristics and physico-chemical properties for the first reported series of DAG lipase (DAGL) inhibitors that function without covalent attachment to the enzyme. Highly potent examples are presented that represent valuable tool compounds for studying DAGL inhibition and constitute important leads for future medicinal chemistry efforts...
April 1, 2016: Bioorganic & Medicinal Chemistry
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