keyword
MENU ▼
Read by QxMD icon Read
search

DAGLα

keyword
https://www.readbyqxmd.com/read/27623930/important-role-of-endocannabinoid-signaling-in-the-development-of-functional-vision-and-locomotion-in-zebrafish
#1
Andrea Martella, Rosa M Sepe, Cristoforo Silvestri, Jingjing Zang, Giulia Fasano, Oliana Carnevali, Paolo De Girolamo, Stephan C F Neuhauss, Paolo Sordino, Vincenzo Di Marzo
The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report the presence of a complete endocannabinoid system during zebrafish development and show that the genes that code for enzymes that catalyze the anabolism and catabolism (mgll and dagla) of the endocannabinoid, 2-AG (2-arachidonoylglycerol), as well as 2-AG main receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino-induced transient knockdown of the zebrafish Daglα homolog and its pharmacologic rescue, we suggest that synthesis of 2-AG is implicated in the control of axon formation in the midbrain-hindbrain region and that animals that lack Daglα display abnormal physiological behaviors in tests that measure stereotyped movement and motion perception...
December 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27595600/regulated-endosomal-trafficking-of-diacylglycerol-lipase-alpha-dagl%C3%AE-generates-distinct-cellular-pools-implications-for-endocannabinoid-signaling
#2
Ya Zhou, Fiona V Howell, Oleg O Glebov, David Albrecht, Gareth Williams, Patrick Doherty
Diacylglycerol lipase alpha (DAGLα) generates the endocannabinoid (eCB) 2-arachidonylglycerol (2-AG) that regulates the proliferation and differentiation of neural stem cells and serves as a retrograde signaling lipid at synapses. Nothing is known about the dynamics of DAGLα expression in cells and this is important as it will govern where 2-AG can be made and released. We have developed a new construct to label DAGLα at the surface of live cells and follow its trafficking. In hippocampal neurons a cell surface pool of DAGLα co-localizes with Homer, a postsynaptic density marker...
October 2016: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/27578612/sex-differences-in-alcohol-consumption-and-alterations-in-nucleus-accumbens-endocannabinoid-mrna-in-alcohol-dependent-rats
#3
Angela M Henricks, Anthony L Berger, Janelle M Lugo, Lydia N Baxter-Potter, Kennedy V Bieniasz, Rebecca M Craft, Ryan J McLaughlin
Chronic intermittent alcohol (CIA) exposure produces altered motivational states characterized by anxiety and escalated alcohol consumption during withdrawal. The endocannabinoid (ECB) system contributes to these symptoms, and sex differences in alcohol dependence, as well as bidirectional interactions between ECBs and gonadal hormones have been documented. Thus, we evaluated sex differences in alcohol consumption, anxiety-like behavior, and ECB mRNA expression in the nucleus accumbens (NAc) of alcohol-dependent rats during acute withdrawal...
October 29, 2016: Neuroscience
https://www.readbyqxmd.com/read/27327781/the-endocannabinoid-system-in-the-baboon-papio-spp-as-a-complex-framework-for-developmental-pharmacology
#4
Iram P Rodriguez-Sanchez, Josee Guindon, Marco Ruiz, Maria E Tejero, Gene Hubbard, Laura E Martinez-de-Villarreal, Hugo A Barrera-Saldaña, Edward J Dick, Anthony G Commuzzie, Natalia E Schlabritz-Loutsevitch
INTRODUCTION: The consumption of marijuana (exogenous cannabinoid) almost doubled in adults during last decade. Consumption of exogenous cannabinoids interferes with the endogenous cannabinoid (or "endocannabinoid" (eCB)) system (ECS), which comprises N-arachidonylethanolamide (anandamide, AEA), 2-arachidonoyl glycerol (2-AG), endocannabinoid receptors (cannabinoid receptors 1 and 2 (CB1R and CB2R), encoded by CNR1 and CNR2, respectively), and synthesizing/degrading enzymes (FAAH, fatty-acid amide hydrolase; MAGL, monoacylglycerol lipase; DAGL-α, diacylglycerol lipase-alpha)...
June 17, 2016: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/27245901/assay-of-dagl%C3%AE-%C3%AE-activity
#5
Tiziana Bisogno
The endocannabinoid 2-arachidonoylglycerol (2-AG) exerts its physiological action by binding to and functionally activating type-1 (CB1) and type-2 (CB2) cannabinoid receptors. It is thought to be produced through the action of sn-1 selective diacylglycerol lipase (DAGL) that catalyzes 2-AG biosynthesis from sn-2-arachidonate-containing diacylglycerols. Since 2-AG biosynthetic enzymes have been identified only recently, little information on methodological approaches for measuring DAGL activity is as yet available...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27167970/fabp-1-gene-ablation-impacts-brain-endocannabinoid-system-in-male-mice
#6
Gregory G Martin, Sarah Chung, Danilo Landrock, Kerstin K Landrock, Huan Huang, Lawrence J Dangott, Xiaoxue Peng, Martin Kaczocha, Drew R Seeger, Eric J Murphy, Mikhail Y Golovko, Ann B Kier, Friedhelm Schroeder
Liver fatty acid-binding protein (FABP1, L-FABP) has high affinity for and enhances uptake of arachidonic acid (ARA, C20:4, n-6) which, when esterified to phospholipids, is the requisite precursor for synthesis of endocannabinoids (EC) such as arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG). The brain derives most of its ARA from plasma, taking up ARA and transporting it intracellularly via cytosolic fatty acid-binding proteins (FABPs 3,5, and 7) localized within the brain. In contrast, the much more prevalent cytosolic FABP1 is not detectable in the brain but is instead highly expressed in the liver...
August 2016: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27130217/quality-of-ehr-data-extractions-for-studies-of-preterm-birth-in-a-tertiary-care-center-guidelines-for-obtaining-reliable-data
#7
Lindsey A Knake, Monika Ahuja, Erin L McDonald, Kelli K Ryckman, Nancy Weathers, Todd Burstain, John M Dagle, Jeffrey C Murray, Prakash Nadkarni
BACKGROUND: The use of Electronic Health Records (EHR) has increased significantly in the past 15 years. This study compares electronic vs. manual data abstractions from an EHR for accuracy. While the dataset is limited to preterm birth data, our work is generally applicable. We enumerate challenges to reliable extraction, and state guidelines to maximize reliability. METHODS: An Epic™ EHR data extraction of structured data values from 1,772 neonatal records born between the years 2001-2011 was performed...
2016: BMC Pediatrics
https://www.readbyqxmd.com/read/27115711/assay-and-inhibition-of-the-purified-catalytic-domain-of-diacylglycerol-lipase-beta
#8
Praveen K Singh, Rachel Markwick, Leanne Lu, Fiona V Howell, Gareth Williams, Patrick Doherty
The diacylglycerol lipases (DAGLα and DAGLβ) hydrolyze DAG to generate 2-arachidonoylglycerol (2-AG), the principal endocannabinoid and main precursor of arachidonic acid (AA). The DAGLs make distinct tissue specific contributions toward 2-AG and AA levels, and therefore, selective modulators for these enzymes could play crucial roles toward harnessing their therapeutic potential. Relatively high-throughput assays have recently been reported for DAGLα and have proven useful toward the characterization of inhibitors of this enzyme...
May 17, 2016: Biochemistry
https://www.readbyqxmd.com/read/27114924/prolonged-activation-of-human-islet-cannabinoid-receptors-in-vitro-induces-adaptation-but-not-dysfunction
#9
Alonso Vilches-Flores, Zara Franklin, Astrid C Hauge-Evans, Bo Liu, Guo C Huang, Pratik Choudhary, Peter M Jones, Shanta J Persaud
BACKGROUND: Although in vivo studies have implicated endocannabinoids in metabolic dysfunction, little is known about direct, chronic activation of the endocannabinoid system (ECS) in human islets. Therefore, this study investigated the effects of prolonged exposure to cannabinoid agonists on human islet gene expression and function. METHODS: Human islets were maintained for 2 and 5 days in the absence or presence of CB1r (ACEA) or CB2r (JWH015) agonists. Gene expression was quantified by RT-PCR, hormone levels by radioimmunoassay and apoptosis by caspase activities...
June 2016: BBA Clinical
https://www.readbyqxmd.com/read/27057735/peripherally-inserted-central-catheters-optimize-nutrient-intake-in-moderately-preterm-infants
#10
Anne L Smazal, Anne B Kavars, Susan J Carlson, Tarah T Colaizy, John M Dagle
BACKGROUND: While very preterm (<32 wk gestation) infants are routinely provided intensive nutritional support via central line, clinical practice varies for nutrient delivery in infants born moderately preterm (32-34 wk gestation). We sought to define the impact of nutritional support via peripherally inserted central catheter (PICC) on nutrient delivery in the first 2 wk of life and growth by discharge. METHODS: Data were extracted from the records of 187 infants born between 32 and 34 6/7 wk gestation and admitted to the University of Iowa Children's Hospital between April 2012 and December 2013...
August 2016: Pediatric Research
https://www.readbyqxmd.com/read/27013337/a-novel-live-cell-assay-to-measure-diacylglycerol-lipase-%C3%AE-activity
#11
Praveen K Singh, Rachel Markwick, Fiona V Howell, Gareth Williams, Patrick Doherty
Diacylglycerol lipase α (DAGLα) hydrolyses DAG to generate the principal endocannabinoid (eCB) 2-arachidonoylglycerol (2-AG) in the central nervous system. DAGLα dependent cannabinoid (CB) signalling has been implicated in numerous processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measurement of diacylglycerol lipase (DAGL) activity using its native substrate generally involves low-throughput MS techniques...
June 2016: Bioscience Reports
https://www.readbyqxmd.com/read/26987678/effects-of-the-antipsychotic-paliperidone-on-stress-induced-changes-in-the-endocannabinoid-system-in-rat-prefrontal-cortex
#12
Karina S MacDowell, Aline Sayd, Borja García-Bueno, Javier R Caso, José L M Madrigal, Juan Carlos Leza
Objectives There is a need to explore novel mechanisms of action of existing/new antipsychotics. One potential candidate is the endocannabinoid system (ECS). The present study tried to elucidate the effects of the antipsychotic paliperidone on stress-induced ECS alterations. Methods Wister rats were submitted to acute/chronic restraint stress. Paliperidone (1 mg/kg) was given prior each stress session. Cannabinoid receptors and endocannabinoids (eCBs) synthesis and degradation enzymes were measured in prefrontal cortex (PFC) samples by RT-PCR and Western Blot...
March 18, 2016: World Journal of Biological Psychiatry
https://www.readbyqxmd.com/read/26977322/a-comparative-analysis-of-the-endocannabinoid-system-in-the-retina-of-mice-tree-shrews-and-monkeys
#13
Joseph Bouskila, Pasha Javadi, Laurent Elkrief, Christian Casanova, Jean-François Bouchard, Maurice Ptito
The endocannabinoid (eCB) system is widely expressed in various parts of the central nervous system, including the retina. The localization of the key eCB receptors, particularly CB1R and CB2R, has been recently reported in rodent and primate retinas with striking interspecies differences. Little is known about the distribution of the enzymes involved in the synthesis and degradation of these eCBs. We therefore examined the expression and localization of the main components of the eCB system in the retina of mice, tree shrews, and monkeys...
2016: Neural Plasticity
https://www.readbyqxmd.com/read/26917221/structure-activity-relationship-studies-on-chemically-non-reactive-glycine-sulfonamide-inhibitors-of-diacylglycerol-lipase
#14
Louis S Chupak, Xiaofan Zheng, Shuanghua Hu, Yazhong Huang, Min Ding, Martin A Lewis, Ryan S Westphal, Yuval Blat, Andrea McClure, Robert G Gentles
N-Benzylic-substituted glycine sulfonamides that reversibly inhibit diacylglycerol (DAG) lipases are reported. Detailed herein are the structure activity relationships, profiling characteristics and physico-chemical properties for the first reported series of DAG lipase (DAGL) inhibitors that function without covalent attachment to the enzyme. Highly potent examples are presented that represent valuable tool compounds for studying DAGL inhibition and constitute important leads for future medicinal chemistry efforts...
April 1, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/26915789/diacylglycerol-lipase-%C3%AE-inhibition-reverses-nociceptive-behaviour-in-mouse-models-of-inflammatory-and-neuropathic-pain
#15
J L Wilkerson, S Ghosh, D Bagdas, B L Mason, M S Crowe, K L Hsu, L E Wise, S G Kinsey, M I Damaj, B F Cravatt, A H Lichtman
BACKGROUND AND PURPOSE: Inhibition of diacylglycerol lipase (DGL)β prevents LPS-induced pro-inflammatory responses in mouse peritoneal macrophages. Thus, the present study tested whether DGLβ inhibition reverses allodynic responses of mice in the LPS model of inflammatory pain, as well as in neuropathic pain models. EXPERIMENTAL APPROACH: Initial experiments examined the cellular expression of DGLβ and inflammatory mediators within the LPS-injected paw pad. DAGL-β (-/-) mice or wild-type mice treated with the DGLβ inhibitor KT109 were assessed in the LPS model of inflammatory pain...
May 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/26811312/cocaine-induced-behavioral-sensitization-decreases-the-expression-of-endocannabinoid-signaling-related-proteins-in-the-mouse-hippocampus
#16
Eduardo Blanco, Pablo Galeano, Ana Palomino, Francisco J Pavón, Patricia Rivera, Antonia Serrano, Francisco Alen, Leticia Rubio, Antonio Vargas, Estela Castilla-Ortega, Juan Decara, Ainhoa Bilbao, Fernando Rodríguez de Fonseca, Juan Suárez
In the reward mesocorticolimbic circuits, the glutamatergic and endocannabinoid systems are implicated in neurobiological mechanisms underlying cocaine addiction. However, the involvement of both systems in the hippocampus, a critical region to process relational information relevant for encoding drug-associated memories, in cocaine-related behaviors remains unknown. In the present work, we studied whether the hippocampal gene/protein expression of relevant glutamate signaling components, including glutamate-synthesizing enzymes and metabotropic and ionotropic receptors, and the hippocampal gene/protein expression of cannabinoid type 1 (CB1) receptor and endocannabinoid metabolic enzymes were altered following acute and/or repeated cocaine administration resulting in conditioned locomotion and locomotor sensitization...
March 2016: European Neuropsychopharmacology: the Journal of the European College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/26779719/a-chemical-proteomic-atlas-of-brain-serine-hydrolases-identifies-cell-type-specific-pathways-regulating-neuroinflammation
#17
Andreu Viader, Daisuke Ogasawara, Christopher M Joslyn, Manuel Sanchez-Alavez, Simone Mori, William Nguyen, Bruno Conti, Benjamin F Cravatt
Metabolic specialization among major brain cell types is central to nervous system function and determined in large part by the cellular distribution of enzymes. Serine hydrolases are a diverse enzyme class that plays fundamental roles in CNS metabolism and signaling. Here, we perform an activity-based proteomic analysis of primary mouse neurons, astrocytes, and microglia to furnish a global portrait of the cellular anatomy of serine hydrolases in the brain. We uncover compelling evidence for the cellular compartmentalization of key chemical transmission pathways, including the functional segregation of endocannabinoid (eCB) biosynthetic enzymes diacylglycerol lipase-alpha (DAGLα) and -beta (DAGLβ) to neurons and microglia, respectively...
2016: ELife
https://www.readbyqxmd.com/read/26773729/the-endocannabinoid-system-in-the-human-granulosa-cell-line-kgn
#18
Jana Ernst, Urszula Grabiec, Thomas Greither, Bernd Fischer, Faramarz Dehghani
Ovarian steroidogenesis is embedded in a sensitive network of regulatory mechanisms crucial for human fertility. The endocannabinoid system (ECS) represents an intrinsic modulating system involved in the regulation of endocrine functions. In the present study we characterized the ECS in the human granulosa cell line KGN and its impact on gonadotropin sensitivity and steroid hormone synthesis under basal and FSH-stimulated conditions. Expression studies were performed and estradiol was measured. CB1, CB2, DAGL, FAAH, GPR55, MAGL, NAPE-PLD and TRPV1 were expressed without FSH-dependent effects...
March 5, 2016: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/26761123/polymorphisms-in-nr5a2-gene-encoding-liver-receptor-homolog-1-are-associated-with-preterm-birth
#19
Dinushan C Kaluarachchi, Allison M Momany, Tamara D Busch, Lucas G Gimenez, Cesar Saleme, Viviana Cosentino, Kaare Christensen, John M Dagle, Kelli K Ryckman, Jeffrey C Murray
BACKGROUND: Preterm birth (PTB) is a major cause of neonatal mortality and morbidity. There is strong evidence of genetic susceptibility. Objective of this study was to identify genetic variants contributing to PTB. METHODS: Genotyping was performed for 24 single nucleotide polymorphisms (SNPs) in 4 candidate genes (NR5A2, FSHR, FOXP3, and SERPINH1). Genotyping was completed on 728 maternal triads (mother and maternal grandparents of a preterm infant). Data were analyzed with Family Based Association Test...
May 2016: Pediatric Research
https://www.readbyqxmd.com/read/26702404/oxyradical-stress-endocannabinoids-and-atherosclerosis
#20
Anberitha T Matthews, Matthew K Ross
Atherosclerosis is responsible for most cardiovascular disease (CVD) and is caused by several factors including hypertension, hypercholesterolemia, and chronic inflammation. Oxidants and electrophiles have roles in the pathophysiology of atherosclerosis and the concentrations of these reactive molecules are an important factor in disease initiation and progression. Overactive NADPH oxidase (Nox) produces excess superoxide resulting in oxidized macromolecules, which is an important factor in atherogenesis. Although superoxide and reactive oxygen species (ROS) have obvious toxic properties, they also have fundamental roles in signaling pathways that enable cells to adapt to stress...
2015: Toxics
keyword
keyword
40074
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"