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fractional laser

Linda van Roij, Appy Sluijs, Jelmer J Laks, Gert-Jan Reichart
RATIONALE: Analyses of stable carbon isotope ratios (δ(13) C values) of organic and inorganic matter remains have been instrumental for much of our understanding of present and past environmental and biological processes. Until recently, the analytical window of such analyses has been limited to samples containing at least several µg of carbon. METHODS: Here we present a setup combining laser ablation, nano combustion gas chromatography and isotope ratio mass spectrometry (LA/nC/GC/IRMS)...
October 21, 2016: Rapid Communications in Mass Spectrometry: RCM
Neha Kumari, Rajesh Kumar, Vandana Mishra, Savita Yadav
CC-NBS-LRR (CNL) plant proteins are related with highly conserved family of disease resistance protein distinguished by a coiled-coil domain, which plays an important role in innate immunity. The present study reports the purification and identification of CNL like protein fragment (CNL-LPF) by two step chromatography and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF/MS), respectively. Furthermore, current study also illustrated the development of polyclonal antibody against purified CNL-LPF, which was used for immunolocalization of CNL-LPF in cytoplasm of cotyledon, using Fluorescence microscopy and Transmission electron microscopy...
October 20, 2016: Protein Journal
J T Oberlerchner, P Vejdovszky, T Zweckmair, A Kindler, S Koch, T Rosenau, A Potthast
Degraded celluloses are becoming increasingly important as part of product streams coming from various biorefinery scenarios. Analysis of the molar mass distribution of such fractions is a challenge, since neither established methods for mono- or disaccharides nor common methods for polysaccharide characterization cover the intermediate oligomer range appropriately. Size exclusion chromatography (SEC) with multi-angle laser light scattering (MALLS), the standard approach for celluloses, suffers from decreased scattering intensities in the lower-molar mass range...
October 8, 2016: Journal of Chromatography. A
Juliana Marioni, María Angel da Silva, José Luis Cabrera, Susana C Núñez Montoya, María Gabriela Paraje
BACKGROUND: Candida tropicalis is increasingly becoming among the most commonly isolated pathogens causing fungal infections with an important biofilm-forming capacity. PURPOSE: This study addresses the antifungal effect of rubiadin (AQ1) and rubiadin 1-methyl ether (AQ2), two photosensitizing anthraquinones (AQs) isolated from Heterophyllaea pustulata, against C. tropicalis biofilms, by studying the cellular stress and antioxidant response in two experimental conditions: darkness and irradiation...
November 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Mi Yu, Jun Young Lee
BACKGROUND: Polydeoxyribonucleotide (PDRN) is an active compound that can promote wound healing. PDRN stimulates wound healing by enhancing angiogenesis and increasing fibroblast growth rates. Laser skin resurfacing is a popular cosmetic procedure for skin rejuvenation. Despite excellent improvement of photo-damaged skin and acne scarring, it is accompanied with drawbacks, such as prolonged erythema and crusting. OBJECTIVE: This study was designed to assess the effect of PDRN on wounds induced by fractional laser resurfacing...
October 20, 2016: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
Jin Ok Baek, Hoon Hur, Ha Ryeong Ryu, Jeong Soo Kim, Kyung Real Lee, Yu Ri Kim, Kwang Ho Choi
Various lasers have been used for the treatment of erythematotelangiectatic rosacea (ETR) that does not respond to systemic or topical therapy. The pulsed dye lasers (PDLs) are an effective option for ETR, and the purpuragenic fluence proved to be superior until now. Given that purpura and subsequent possible postinflammatory hyperpigmentation (PIH) are occasionally unbearable in some patients, and several studies using the low nonpurpuragenic fluence were reported. To deliver the sufficient high fluence of a PDL without generating purpura, we designed the fractionation of high fluence using five passes and longer pulse duration (6 milliseconds) of a PDL in succession...
October 20, 2016: Journal of Cosmetic Dermatology
Alina Constantin, Madalina Dumitrescu, Maria Cristina Mihai Corotchi, Dana Jianu, Maya Simionescu
CO2 laser has a beneficial effect on stem cells by mechanisms that are not clearly elucidated. We hypothesize that the effect of fractional CO2 laser on human adipose-derived stem cells (ADSC) could be due to changes in redox homeostasis and secretion of factors contributing to cellular proliferation and angiogenic potential. ADSC incubated in medium containing 0.5 or 10 % FBS were exposed to a single irradiation of a 10,600-nm fractional CO2 laser; non-irradiated ADSC were used as control. Viability/proliferation of ADSC was assessed by MTT assay; the intracellular reactive oxygen species (ROS) levels and the mitochondrial membrane potential (∆Ψm) were determined with DCFH-DA and JC-1 fluorescent probes, respectively...
October 20, 2016: Lasers in Medical Science
Bernard Lavielle, Bertrand Thomas, Eric Gilabert, Gregory Canchel, Denis Horlait, Sylvain Topin, Fabien Pointurier, Christophe Moulin
A double focusing sector field mass filter used in Nier-Johnson geometry has been built in order to perform Kr isotope enrichment for (81) Kr and (85) Kr isotopes. The principle consists in implanting Kr(+) ions accelerated at 7 keV in Al foils after separation using the magnetic sector. A specific ion source has been designed capable of generating high Kr(+) ion beams (>0.5 μA) to transfer into the collecting Al foils in 3 to 5 h significant fractions of large Kr samples (10(15) to 10(16) atoms) initially introduced in the instrument...
October 2016: Journal of Mass Spectrometry: JMS
So Eun Park, Sang Seok Kim, Chul Woo Kim, Young Her
No abstract text is available yet for this article.
October 2016: Annals of Dermatology
Qian Li, Rui Liu, Tao Wu, Min Zhang
Six soluble dietary fiber (SDF) fractions were prepared via stepwise ethanol precipitation from natural and fermented wheat bran. The chemical composition, molecular weight distribution, glycosidic linkage and substitution pattern of the SDF fractions were elucidated by sugar analysis, periodate oxidation and Smith degradation, molecular determination, and 1H nuclear magnetic resonance (NMR) analysis. The impacts of SDF fractions on the rheological properties and morphologies of doughs were investigated by farinography, rheometry and scanning electron microscopy (SEM) to clarify the relationship between the microstructural features of SDF fractions and the macroscopic properties of SDF-containing doughs...
October 17, 2016: Journal of Agricultural and Food Chemistry
Alba Hykollari, Katharina Paschinger, Barbara Eckmair, Iain B H Wilson
N-glycans from invertebrates and protists have often unusual structures which present analytical challenges. Both core and antennal modifications can be quite different from the more familiar vertebrate glycan motifs; thereby, contrary to the concept that "simple" organisms have "simple" N-glycans, rather complex oligosaccharides structures, including zwitterionic and anionic ones, have been found in a range of species. Thus, to facilitate the optimized elucidation of the maximal possible range of structures, the analytical workflow for glycomics of these organisms should include sequential release and fractionation steps...
2017: Methods in Molecular Biology
K M Ratheesh, L K Seah, V M Murukeshan
The automatic calibration in Fourier-domain optical coherence tomography (FD-OCT) systems allows for high resolution imaging with precise depth ranging functionality in many complex imaging scenarios, such as microsurgery. However, the accuracy and speed of the existing automatic schemes are limited due to the functional approximations and iterative operations used in their procedures. In this paper, we present a new real-time automatic calibration scheme for swept source-based optical coherence tomography (SS-OCT) systems...
October 14, 2016: Physics in Medicine and Biology
Sarah Qureshi, Jennifer Y Lin
Aminolevulinic acid (ALA) photodynamic therapy (PDT) is an emerging modality in the treatment of acne. While ablative fractional lasers have been used to enhance drug delivery into the epidermis, recent evidence suggests that non-ablative fractional photothermolysis may also improve uptake of ALA. We explored the use of non-ablative 1550 nm laser as a safe alternative in the delivery of ALA prior to red-light PDT for refractory inflammatory and cystic acne. Subjects referred for treatment of acne refractory to several topical and oral regimens, including isotretinoin, were pre-treated with non-ablative fractional photothermolysis (NAFP)...
October 14, 2016: Lasers in Medical Science
Elia Marin, Alfredo Rondinella, Wenliang Zhu, Bryan J McEntire, B Sonny Bal, Giuseppe Pezzotti
The effect of frictional sliding on the surface degradation of commercially available zirconia-toughened alumina (ZTA) femoral heads was studied using a pin-on-ball wear tester under three different lubricating conditions: dry, water, and squalene. Water and squalene were employed under boundary lubrication regimes. Despite the unique (non-standard) character of this apparatus, standard loading conditions could be applied, which effectively determined dynamic friction coefficients as basic material properties...
September 28, 2016: Journal of the Mechanical Behavior of Biomedical Materials
Joachim Vater, Stefanie Herfort, Joerg Doellinger, Max Weydmann, Kristin Dietel, Sebastian Faetke, Peter Lasch
Paenibacillus polymyxa are rhizobacteria with a high potential to produce natural compounds of biotechnological and medical interest. Main products of P. polymyxa are fusaricidins, a large family of antifungal lipopeptides with a 15-guanidino-3-hydroxypentadecanoic acid (GHPD) as fatty acid side chain. We use the P. polymyxa strain M-1 as a model organism for the exploration of the biosynthetic potential of these rhizobacteria. Using matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) about 40 new fusaricidins were detected which were fractionated by reversed-phase (rp) HPLC...
October 6, 2016: Journal of Mass Spectrometry: JMS
William R Holmes, Adriana E Golding, William M Bement, Leah Edelstein-Keshet
Rho GTPases are regulatory proteins whose patterns on the surface of a cell affect cell polarization, cell motility and repair of single-cell wounds. The stereotypical patterns formed by two such proteins, Rho and Cdc42, around laser-injured frog oocytes permit experimental analysis of GTPase activation, inactivation, segregation and crosstalk. Here, we review the development and analysis of a spatial model of GTPase dynamics that describe the formation of concentric zones of Rho and Cdc42 activity around wounds, and describe how this model has provided insights into the roles of the GTPase effector molecules protein kinase C (PKCβ and PKCη) and guanosine nucleotide dissociation inhibitor (GDI) in the wound response...
October 6, 2016: Interface Focus
Andrea C Issler-Fisher, Oliver M Fisher, Ania O Smialkowski, Frank Li, Constant P van Schalkwyk, Peter Haertsch, Peter K M Maitz
BACKGROUND: The introduction of ablative fractional CO2 lasers (CO2-AFL) for burn scar management shows promising results. Whilst recent studies have focused on objective scar outcomes following CO2-AFL treatment, to date no data on patient subjective factors such as quality of life are available. METHODS: A prospective study was initiated to analyze the safety and efficacy of the CO2-AFL. Various objective and subjective outcome parameters were prospectively collected from the date of first consultation and follow-up following treatment...
October 1, 2016: Burns: Journal of the International Society for Burn Injuries
Blair M Goodridge, David L Valentine
Measurements of the isotopic composition of sulfate and sulfide have been essential to uncovering the microbial and geochemical processes that drive Earth's sulfur cycle. These processes routinely operate over submillimeter spatial scales in anaerobic environments, necessitating sulfur isotopic measurement techniques that can identify isotopic variability over equivalently small scales. Here we present a new method for small-scale (10(2) μm) measurements of δ(34)S using black and white photographic film sulfide capture coupled with δ(34)S determination using laser ablation multicollector inductively coupled plasma mass spectrometry...
October 5, 2016: Analytical Chemistry
Ningning Ma, Yao-Wen Jiang, Xiaodong Zhang, Hao Wu, John N Myers, Peidang Liu, Haizhen Jin, Ning Gu, Nongyue He, Fu-Gen Wu, Zhan Chen
Metallic nanostructures as excellent candidates for nanosensitizers have shown enormous potentials in cancer radiotherapy and photothermal therapy. Clinically, a relatively low and safe radiation dose is highly desired to avoid damage to normal tissues. Therefore, the synergistic effect of the low-dosed X-ray radiation and other therapeutic approaches (or so-called "combined therapeutic strategy") is needed. Herein, we have synthesized hollow and spike-like gold nanostructures by a facile galvanic replacement reaction...
October 14, 2016: ACS Applied Materials & Interfaces
Frederik Crop, David Pasquier, Amandine Baczkiewic, Julie Doré, Lena Bequet, Emeline Steux, Anne Gadroy, Jacqueline Bouillon, Clement Florence, Laurence Muszynski, Mathilde Lacour, Eric Lartigau
A surface imaging system, Catalyst (C-Rad), was compared with laser-based positioning and daily mega voltage computed tomography (MVCT) setup for breast patients with nodal involvement treated by helical TomoTherapy. Catalyst-based positioning performed better than laser-based positioning. The respective modalities resulted in a standard deviation (SD), 68% confidence interval (CI) of positioning of left-right, craniocaudal, anterior-posterior, roll: 2.4 mm, 2.7 mm, 2.4 mm, 0.9° for Catalyst positioning, and 6...
2016: Journal of Applied Clinical Medical Physics
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