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https://www.readbyqxmd.com/read/28340289/remote-ischaemic-conditioning-decreases-blood-flow-and-improves-oxygen-extraction-in-patients-with-early-complex-regional-pain-syndrome
#1
T Hegelmaier, N Kumowski, T Mainka, J Vollert, O Goertz, M Lehnhardt, P K Zahn, C Maier, J Kolbenschlag
BACKGROUND: Remote ischaemic conditioning (RIC) is the cyclic application of non-damaging ischaemia leading to an increased tissue perfusion, among others triggered by NO (monoxide). Complex regional pain syndrome (CRPS) is known to have vascular alterations such as increased blood shunting and decreased NO blood-levels, which in turn lead to decreased tissue perfusion. We therefore hypothesized that RIC could improve tissue perfusion in CRPS. METHOD: In this proof-of-concept study, RIC was applied in the following groups: in 21 patients with early CRPS with a clinical history less than a year, in 20 age/sex-matched controls and in 12 patients with unilateral nerve lesions via a tourniquet on the unaffected/non-dominant upper limb...
March 24, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/28333369/in-vivo-multiphoton-microscopy-of-picosecond-laser-induced-optical-breakdown-in-human-skin
#2
Mihaela Balu, Griffin Lentsch, Dorota Z Korta, Karsten König, Kristen M Kelly, Bruce J Tromberg, Christopher B Zachary
IMPORTANCE: Improvements in skin appearance resulting from treatment with fractionated picosecond-lasers have been noted, but optimizing the treatment efficacy depends on a thorough understanding of the specific skin response. The development of non-invasive laser imaging techniques in conjunction with laser therapy can potentially provide feedback for guidance and optimizing clinical outcome. OBJECTIVE: The purpose of this study was to demonstrate the capability of multiphoton microscopy (MPM), a high-resolution, label-free imaging technique, to characterize in vivo the skin response to a fractionated non-ablative picosecond-laser treatment...
March 23, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28330352/thermal-conductivity-of-the-sideledge-in-aluminium-electrolysis-cells-experiments-and-numerical-modelling
#3
Aïmen E Gheribi, Sándor Poncsák, Sébastien Guérard, Jean-François Bilodeau, László Kiss, Patrice Chartrand
During aluminium electrolysis, a ledge of frozen electrolytes is generally formed, attached to the sides of the cells. This ledge acts as a protective layer, preventing erosion and chemical attacks of both the electrolyte melt and the liquid aluminium on the side wall materials. The control of the sideledge thickness is thus essential in ensuring a reasonable lifetime for the cells. The key property for modelling and predicting the sideledge thickness as a function of temperature and electrolyte composition is the thermal conductivity...
March 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28322149/characterization-of-polysaccharides-from-the-fruiting-bodies-of-two-species-of-genus-ganoderma-agaricomycetes-and-determination-of-water-soluble-%C3%AE-d-glucan-using-high-performance-liquid-chromatography
#4
Yanfang Liu, Qingjiu Tang, Yan Yang, Shuai Zhou, Di Wu, Chuanhong Tang, Zhong Zhang, Mengqiu Yan, Jie Feng, Jing-Song Zhang
Molecular weight (Mw) distributions of polysaccharides from the fruiting bodies of different Ganoderma lucidum strains and G. sinense were investigated and compared using high-pressure size exclusion chromatography/multiangle laser light scattering/refractive index analysis. Results showed that there were big differences in the Mw distributions and characteristics of polysaccharides from 2 species of Ganoderma. All tested G. lucidum materials exhibited similar polysaccharide distributions and similar characteristics for each fraction...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28319290/role-of-dissolved-and-molecular-oxygen-on-cu-and-ptcu-alloy-particle-structure-during-laser-ablation-synthesis-in-liquids
#5
Galina Marzun, Helmut Bönnemann, Christian Lehmann, Bernd Spliethoff, Claudia Weidenthaler, Stephan Barcikowski
The role of molecular oxygen dissolved in the solvent is often discussed as being an influential factor on particle oxidation during pulsed laser ablation in liquids. However, the formation of the particles during laser synthesis takes place under extreme conditions that enable the decomposition of the liquid medium. Reactive species of the solvent may then affect particle formation due to a chemical reaction in the reactive plasma. Experimental results show a difference between the role of dissolved molecular oxygen and the contribution from the oxygen in water molecules...
March 20, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28316983/endovenous-laser-ablation-of-varicose-veins-preserves-biological-properties-of-vascular-endothelium-and-modulates-proinflammatory-agent-profile-more-favorably-than-classic-vein-stripping
#6
Paweł Uruski, Krzysztof Aniukiewicz, Justyna Mikuła-Pietrasik, Patrycja Sosińska, Andrzej Tykarski, Krzysztof Książek, Zbigniew Krasiński
Here we compared effect of serum from varicose patients undergoing endovenous laser ablation (EVLA) and classic vein stripping (CVS) on biological properties of endothelial cells and on the local and systemic profiles of proinflammatory agents. Results showed that serum from EVLA patients improved proliferation and reduced senescence and oxidative stress in the endothelial cells, as compared with the serum from CVS patients. These effects were related to a suppressed activity of TGF-β1, the level of which in the serum from the EVLA patients was decreased...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28316243/underwater-transparent-miniature-mechanical-hand-based-on-femtosecond-laser-induced-controllable-oil-adhesive-patterned-glass-for-oil-droplet-manipulation
#7
Jinglan Huo, Qing Yang, Feng Chen, Jiale Yong, Yao Fang, Jingzhou Zhang, Lin Liu, Xun Hou
The development of underwater superoleophobic surfaces makes researchers be swept up in boundless imagination of their applications, thereinto the oil manipulation based on such surface attracts much attention. Here, we show a simple and effective way to fabricate an underwater transparent miniature "mechanical hand" based on controllable oil-adhesive patterned glass prepared by femtosecond laser. The underwater oil-adhesive force of the patterned glasses that compose the "mechanical hand" device can be controlled from ultralow to ultrahigh through adjusting the different fraction of the untreated flat glass area to the laser-ablated rough area...
March 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28314913/laser-and-light-based-therapy-for-cutaneous-and-soft-tissue-metastases-of-malignant-melanoma-a-systematic-review
#8
REVIEW
Evan Austin, Andrew Mamalis, Derek Ho, Jared Jagdeo
Invasive cutaneous melanoma is a growing health concern. Although surgical excision can effectively treat in situ tumors, use for metastatic melanoma is limited. Laser and light-based therapies may be a valuable palliative treatment option for patients with stage III and stage IV cutaneous metastatic melanoma. Our goal is to review the published literature and provide evidence-based recommendations on laser and light-based palliative therapies for metastatic melanoma. A search of the databases Pubmed, EMBASE, Web of Science, and CINAHL was performed on March 10, 2016...
March 17, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28299642/cerebromicrovascular-dysfunction-predicts-cognitive-decline-and-gait-abnormalities-in-a-mouse-model-of-whole-brain-irradiation-induced-accelerated-brain-senescence
#9
Zoltan Ungvari, Stefano Tarantini, Peter Hertelendy, M Noa Valcarcel-Ares, Gabor A Fülöp, Sreemathi Logan, Tamas Kiss, Eszter Farkas, Anna Csiszar, Andriy Yabluchanskiy
Whole brain irradiation (WBI) is a mainstream therapy for patients with both identifiable brain metastases and prophylaxis for microscopic malignancies. However, it also promotes accelerated senescence in healthy tissues and leads to progressive cognitive dysfunction in up to 50% of tumor patients surviving long term after treatment, due to γ-irradiation-induced cerebromicrovascular injury. Moment-to-moment adjustment of cerebral blood flow (CBF) via neuronal activity-dependent cerebromicrovascular dilation (functional hyperemia) has a critical role in maintenance of healthy cognitive function...
February 2017: Geroscience
https://www.readbyqxmd.com/read/28299490/molecular-effects-of-fractional-ablative-erbium-yag-laser-treatment-with-multiple-stacked-pulses-on-standardized-human-three-dimensional-organotypic-skin-models
#10
Laurenz Schmitt, P M Amann, Y Marquardt, R Heise, K Czaja, P A Gerber, T Steiner, F Hölzle, Jens Malte Baron
The molecular changes in gene expression following ablative laser treatment of skin lesions, such as atrophic scars and UV-damaged skin, are not completely understood. A standardized in vitro model of human skin, to study the effects of laser treatment on human skin, has been recently developed. Therefore, the aim of the investigation was to examine morphological and molecular changes caused by fractional ablative erbium:YAG laser treatment on an in vitro full-thickness 3D standardized organotypic model of human skin...
March 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28297444/surface-imaging-laser-positioning-or-volumetric-imaging-for-breast-cancer-with-nodal-involvement-treated-by-helical-tomotherapy
#11
Frederik Crop, David Pasquier, Amandine Baczkiewic, Julie Doré, Lena Bequet, Emeline Steux, Anne Gadroy, Jacqueline Bouillon, Clement Florence, Laurence Muszynski, Mathilde Lacour, Eric Lartigau
A surface imaging system, Catalyst (C-Rad), was compared with laser-based positioning and daily mega voltage computed tomography (MVCT) setup for breast patients with nodal involvement treated by helical TomoTherapy. Catalyst-based positioning performed better than laser-based positioning. The respective modalities resulted in a standard deviation (SD), 68% confidence interval (CI) of positioning of left-right, craniocaudal, anterior-posterior, roll: 2.4 mm, 2.7 mm, 2.4 mm, 0.9° for Catalyst positioning, and 6...
September 2016: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28295433/structural-analysis-and-enhanced-production-of-fusaricidin-from-paenibacillus-kribbensis-cu01-isolated-from-yellow-loess
#12
Jaewon Ryu, Jong M Kim, Chul W Lee, Si W Kim
A bacterial strain showing strong antifungal activity was isolated from yellow loess and was identified as Paenibacillus kribbensis CU01. Insoluble mucoidal polymers were separated from M9 culture medium via low-speed centrifugation. Most antifungal activity was associated with substances in the insoluble precipitate, which was purified by reverse phase high performance liquid chromatography. Purified fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry...
March 10, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28294302/polyclonal-and-monoclonal-igg-binding-on-protein-a-resins-evidence-of-competitive-binding-effects
#13
Justin Weinberg, Shaojie Zhang, Gillian Crews, Edward Healy, Giorgio Carta, Todd Przybycien
Protein A (ProA) chromatography is used extensively in the biopharmaceutical industry for the selective capture of both polyclonal and monoclonal antibodies (mAbs). This work provides a comparison of the adsorptive behavior of a highly heterogeneous polyclonal hIgG versus that of a mAb as well as the behavior of their mixtures on representative ProA resins. Both pH gradient elution and frontal loading experiments using human polyclonal IgG (hIgG) reveal a distribution of IgG-ProA binding strengths likely associated with multiple IgG subclasses and the heterogeneity of the variable region...
March 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28290283/ace-inhibitory-peptides-from-bovine-caseins-released-with-peptidases-from-maclura-pomifera-latex
#14
María Alicia Corrons, Constanza Silvina Liggieri, Sebastián Alejandro Trejo, Mariela Anahí Bruno
In work reported here, a proteolytic extract prepared from Maclura pomifera latex was employed to hydrolyze bovine caseins. Densitograms of Tricine-sodium-dodecyl-sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) indicated that the caseins were considerably degraded after a 10-min reaction. The degree of hydrolysis determined by the 2,4,6-trinitrobenzenesulfonic-acid method was 17.1±0.7% after 180min of digestion. The concentration of small peptides increased with hydrolysis time, and analysis by reverse-phase high-performance liquid chromatography (RP HPLC) and mass spectrometry, revealed a virtually unchanged peptide profile...
March 2017: Food Research International
https://www.readbyqxmd.com/read/28287592/chemical-analysis-of-water-accommodated-fractions-of-crude-oil-spills-using-tims-ft-icr-ms
#15
Paolo Benigni, Rebecca Marin, Kathia Sandoval, Piero Gardinali, Francisco Fernandez-Lima
Multiple chemical processes control how crude oil is incorporated into seawater and also the chemical reactions that occur overtime. Studying this system requires the careful preparation of the sample in order to accurately replicate the natural formation of the water-accommodated fraction that occurs in nature. Low-energy water-accommodated fractions (LEWAF) are carefully prepared by mixing crude oil and water at a set ratio. Aspirator bottles are then irradiated, and at set time points, the water is sampled and extracted using standard techniques...
March 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28282259/study-on-the-pulmonary-delivery-system-of-apigenin-loaded-albumin-nanocarriers-with-antioxidant-activity
#16
Zsófia Edit Pápay, Annamária Kósa, Béla Böddi, Zahra Merchant, Imran Y Saleem, Mohammed Gulrez Zariwala, Imre Klebovich, Satyanarayana Somavarapu, István Antal
BACKGROUND: Respiratory diseases are mainly derived from acute and chronic inflammation of the alveoli and bronchi. The pathophysiological mechanisms of pulmonary inflammation mainly arise from oxidative damage that could ultimately lead to acute lung injury. Apigenin (Api) is a natural polyphenol with prominent antioxidant and anti-inflammatory properties in the lung. Inhalable formulations that consist of nanoparticles (NPs) have several advantages over other administration routes, and therefore, this study investigated the application of apigenin-loaded bovine serum albumin nanoparticles (BSA-Api-NPs) for pulmonary delivery...
March 10, 2017: Journal of Aerosol Medicine and Pulmonary Drug Delivery
https://www.readbyqxmd.com/read/28281359/changes-in-skin-microcirculation-during-radiation-therapy-for-breast-cancer
#17
Erik Tesselaar, Anna M Flejmer, Simon Farnebo, Alexandru Dasu
BACKGROUND: The majority of breast cancer patients who receive radiation treatment are affected by acute radiation-induced skin changes. The assessment of these changes is usually done by subjective methods, which complicates the comparison between different treatments or patient groups. This study investigates the feasibility of new robust methods for monitoring skin microcirculation to objectively assess and quantify acute skin reactions during radiation treatment. MATERIAL AND METHODS: Laser Doppler flowmetry, laser speckle contrast imaging, and polarized light spectroscopy imaging were used to measure radiation-induced changes in microvascular perfusion and red blood cell concentration (RBC) in the skin of 15 patients undergoing adjuvant radiation therapy for breast cancer...
March 10, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28277004/fractional-laser-assisted-percutaneous-drug-delivery-via-temperature-responsive-liposomes
#18
Takahiro Fujimoto, Masayuki Ito, Shinobu Ito, Hideko Kanazawa
Liposomes are used for transdermal delivery of drugs and vaccines. Our objective was to develop temperature-responsive (TR) liposomes to achieve temperature-dependent, controlled release of an encapsulated drug, and use fractional laser irradiation to enhance transdermal permeability of these liposomes. TR-liposomes prepared using a thermosensitive polymer derived from poly-N-isopropylacrylamide, N,N-dimethylacrylamide, egg phosphatidylcholine, and dioleoylphosphatidylethanolamine, delivered fluorescein isothiocyanate-conjugated ovalbumin (OVA-FITC) as a model drug...
May 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28276783/application-of-cyclodextrins-in-antibody-microparticles-potentials-for-antibody-protection-in-spray-drying
#19
Vahid Ramezani, Alireza Vatanara, Mohammad Seyedabadi, Mohsen Nabi Meibodi, Hamed Fanaei
OBJECTIVES: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG. METHODS: D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively...
March 1, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28274415/characterization-of-maillard-type-lysozyme-galactomannan-conjugate-having-immune-enhancing-effects
#20
Jae-Eon Yang, Su-Hyun Chun, Ha Hyung Kim, Hee-Don Choi, Kwang-Won Lee
In the present study, lysozyme-galactomannan conjugate (LGC) was fractionated by ion-exchange chromatography, the immune activity of the fractions was confirmed, and a structural analysis of the glycoprotein was performed. A high-molecular-weight fraction of LGC (H-LGC), was characterized by using a method using matrix-assisted laser desorption/ionization time of flight mass spectrometry. The glycated site of H-LGC was determined to be the lysine (Lys)115 residue. In addition, about 1mol of galactomannan (G) was linked to 1mol of lysozyme (L) in LGC based on the binding weight ratio...
July 15, 2017: Food Chemistry
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