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https://www.readbyqxmd.com/read/28213875/vehicle-type-affects-filling-of-fractional-laser-ablated-channels-imaged-by-optical-coherence-tomography
#1
Uffe Høgh Olesen, Mette Mogensen, Merete Haedersdal
Ablative fractional laser (AFXL) is an emerging method that enhances topical drug delivery. Penetrating the skin in microscopic, vertical channels, termed microscopic treatment zones (MTZs), the fractional technique circumvents the skin barrier and allows increased uptake of topically applied drugs. This study aims to elucidate the impact of vehicle type on the filling of MTZs from application of liquid, gel, and cream vehicles. Ex vivo pig skin was exposed to 10,600 nm fractional CO2 laser at 5% density, 120 μm beam diameter, and fluences of 40 and 80 mJ/microbeam (mJ/mb)...
February 17, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28212705/detection-of-dna-damage-by-space-radiation-in-human-fibroblasts-flown-on-the-international-space-station
#2
Tao Lu, Ye Zhang, Michael Wong, Alan Feiveson, Ramona Gaza, Nicholas Stoffle, Huichen Wang, Bobby Wilson, Larry Rohde, Louis Stodieck, Fathi Karouia, Honglu Wu
Although charged particles in space have been detected with radiation detectors on board spacecraft since the discovery of the Van Allen Belts, reports on the effects of direct exposure to space radiation in biological systems have been limited. Measurement of biological effects of space radiation is challenging due to the low dose and low dose rate nature of the radiation environment, and due to the difficulty in distinguishing the radiation effects from microgravity and other space environmental factors. In astronauts, only a few changes, such as increased chromosome aberrations in their lymphocytes and early onset of cataracts, are attributed primarily to their exposure to space radiation...
February 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28210386/ionic-hydrogel-monotherapy-and-in-combination-with-antiviral-antibiotic-prophylaxis-in-the-post-procedure-management-of-fractional-laser-treated-patients
#3
Francesca Romana Grippaudo
Objective: The pre- and postoperative care for fractionated laser resurfacing is still controversial, especially in regard to the use of antibiotics to prevent bacterial infection and potential consequences. Recently, an ionic hydrogel has shown to be useful in the postoperative treatment of minor burns. The aim of this study is to evaluate the use of this hydrogel after fractional laser treatments targeting photoaging and chronoaging damage to skin. Design: A randomized prospective study. Setting: one plastic surgeon private practice...
November 2016: Journal of Clinical and Aesthetic Dermatology
https://www.readbyqxmd.com/read/28207466/the-subunit-principle-in-scar-face-revision
#4
Ahmed Elshahat, Riham Lashin
Facial scaring is considered one of the most difficult cosmetic problems for any plastic surgeon to solve. The condition is more difficult if the direction of the scar is not parallel to relaxed skin tension lines. Attempts to manage this difficult situation included revisions using geometric designs, Z plasties or W plasties to camouflage the straight line visible scaring. The use of long-lasting resorbable sutures was tried too. Recently, the use of botulinum toxin during revision improved the results. Fractional CO2 lasers, microfat grafts, and platelet-rich plasma were added to the armamentarium...
February 15, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28199463/effect-of-methyl-aminolevulinate-photodynamic-therapy-with-and-without-ablative-fractional-laser-treatment-in-patients-with-microinvasive-squamous-cell-carcinoma-a-randomized-clinical-trial
#5
Seung-Hwan Choi, Ki-Ho Kim, Ki-Hoon Song
Importance: Surgical excision is the standard treatment for cutaneous squamous cell carcinoma (SCC). However, microinvasive SCC (Clark level II) is limited to the papillary dermis, and it should be differentiated from invasive SCC. Ablative fractional laser-primed photodynamic therapy (AFL-PDT) may have enhanced efficacy. Objective: To compare 1 session of AFL-PDT with 2 sessions of conventional methyl aminolevulinate-PDT (MAL-PDT) for the treatment of microinvasive SCC...
February 15, 2017: JAMA Dermatology
https://www.readbyqxmd.com/read/28196755/characterization-of-particle-emission-from-laser-printers
#6
Mauro Scungio, Tania Vitanza, Luca Stabile, Giorgio Buonanno, Lidia Morawska
Emission of particles from laser printers in office environments is claimed to have impact on human health due to likelihood of exposure to high particle concentrations in such indoor environments. In the present paper, particle emission characteristics of 110 laser printers from different manufacturers were analyzed, and estimations of their emission rates were made on the basis of measurements of total concentrations of particles emitted by the printers placed in a chamber, as well as particle size distributions...
February 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28194967/multicellular-tumor-spheroids-combined-with-mass-spectrometric-histone-analysis-to-evaluate-epigenetic-drugs
#7
Peter E Feist, Simone Sidoli, Xin Liu, Monica M Schroll, Sharif Rahmy, Rina Fujiwara, Benjamin A Garcia, Amanda B Hummon
Multicellular tumor spheroids (MCTS) are valuable in vitro tumor models frequently used to evaluate the penetration and efficacy of therapeutics. In this study, we evaluated potential differences in epigenetic markers, i.e. histone post-translational modifications (PTMs), in the layers of the HCT116 colon carcinoma MCTS. Cells were grown in agarose-coated 96 well plates, forming reproducible 1mm diameter MCTS. The MCTS were fractionated into three radially concentric portions, generating samples containing cells from the core, the mid and the external layers...
February 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28194938/extension-of-the-kendrick-mass-defect-analysis-of-homopolymers-to-low-resolution-and-high-mass-range-mass-spectra-using-fractional-base-units
#8
Thierry Fouquet, Hiroaki Sato
Beyond the high resolution/low mass range data traditionally used, a Kendrick mass defect analysis (KMD) using the new concept of fractional base units has been successfully conducted on low resolution/low mass range and high resolution/high mass range data for the first time. Relying on a mathematical framework to rationalize the effect of the fractional base units, the electrospray ionization single stage and multistage mass spectra of a poly(vinylpyrrolidone) recorded from a low resolution ion trap analyzer were turned into information-rich KMD plots using vinylpyrrolidone/112 and pyrrolidone/86 as base units...
February 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28194115/low-level-laser-application-in-the-early-myocardial-infarction-stage-has-no-beneficial-role-in-heart-failure
#9
Martha T Manchini, Ednei L Antônio, José Antônio Silva Junior, Paulo de Tarso C de Carvalho, Regiane Albertini, Fernando C Pereira, Regiane Feliciano, Jairo Montemor, Stella S Vieira, Vanessa Grandinetti, Amanda Yoshizaki, Marcio Chaves, Móises P da Silva, Rafael do Nascimento de Lima, Danilo S Bocalini, Bruno L de Melo, Paulo J F Tucci, Andrey J Serra
Low-level laser therapy (LLLT) has been targeted as a promising approach that can mitigate post-infarction cardiac remodeling. There is some interesting evidence showing that the beneficial role of the LLLT could persist long-term even after the end of the application, but it remains to be systematically evaluated. Therefore, the present study aimed to test the hypothesis that LLLT beneficial effects in the early post-infarction cardiac remodeling could remain in overt heart failure even with the disruption of irradiations...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28190356/hydroperoxide-measurements-during-low-temperature-gas-phase-oxidation-of-n-heptane-and-n-decane
#10
Anne Rodriguez, Olivier Herbinet, Xiangzan Meng, Christa Fittschen, Zhandong Wang, Lili Xing, Lidong Zhang, Frédérique Battin-Leclerc
A wide range of hydroperoxides (C1-C3 alkyl hydroperoxides, C3-C7 alkenyl hydroperoxides, C7 ketohydroperoxides and hydrogen peroxide (H2O2)), as well as ketene and diones, have been quantified during the gas-phase oxidation of n-heptane. Some of these species, as well as C10 alkenyl hydroperoxides and ketohydroperoxides, were also measured during the oxidation of n-decane. These experiments were was performed using an atmospheric jet-stirred reactor at temperatures from 500 to 1100 K and one of three analytical methods: time-of-flight mass spectrometry combined with tunable synchrotron photoionization with a molecular beam sampling: time-of-flight mass spectrometry combined with laser photoionization with a capillary tube sampling, continuous wave cavity ring-down spectroscopy (cw-CRDS) with sonic probe sampling...
February 13, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28190338/towards-streamlined-identification-of-dioxin-like-compounds-in-environmental-samples-through-integration-of-suspension-bioassay
#11
Hongxia Xiao, Markus Brinkmann, Beat Thalmann, Andreas Schiwy, Sigrid Große Brinkhaus, Christine Achten, Kathrin Eichbaum, Carolin Gembé, Thomas Benjamin Seiler, Henner Hollert
Effect-directed analysis (EDA) is a powerful strategy to identify biologically active compounds in environmental samples. However, in current EDA studies, fractionation and handling procedures are laborious, consist of multiple evaporation steps, and thus bear the risk of contamination and decreased recoveries of the target compounds. The low resulting throughput has been one of the major bottlenecks of EDA. Here, we propose a high-throughput EDA (HT-EDA) work-flow combining reversed phase high-performance liquid chromatography (HPLC) fractionation of samples into 96-well microplates, followed by toxicity assessment in the micro-EROD bioassay with the wild-type rat hepatoma H4IIE cells, and chemical analysis of bioactive fractions...
February 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28181723/nanosecond-laser-assisted-nitrogen-doping-of-graphene-oxide-dispersions
#12
Dejan Kepić, Stefania Sandoval, Ángel Pérez Del Pino, Enikö György, Laura Cabana, Belén Ballesteros, Gerard Tobias
N-doped reduced graphene oxide (RGO) has been prepared in bulk form by laser irradiation of graphene oxide (GO) dispersed in an aqueous solution of ammonia. A pulsed Nd:YAG laser with emission wavelengths in the infrared (IR) 1064 nm, visible (VIS) 532 nm, and ultraviolet (UV) 266 nm spectral regions was employed for the preparation of the N-doped RGO samples. Regardless of the laser energy employed, the resulting material presents a higher fraction of pyrrolic nitrogen compared to nitrogen atoms in pyridinic and graphitic coordination...
February 9, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28181673/laser-induced-thermal-coagulation-enhances-skin-uptake-of-topically-applied-compounds
#13
C S Haak, J Hannibal, U Paasch, R R Anderson, M Haedersdal
BACKGROUND: Ablative fractional laser (AFL) generates microchannels in skin surrounded by a zone of thermally altered tissue, termed the coagulation zone (CZ). The thickness of CZ varies according to applied wavelength and laser settings. It is well-known that AFL channels facilitate uptake of topically applied compounds, but the importance of CZ is unknown. METHODS: Franz Cells were used to investigate skin uptake and permeation of fluorescent labeled polyethylene glycols (PEGs) with mean molecular weights (MW) of 350, 1,000, and 5,000 Da...
February 9, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28169913/use-of-a-novel-fractional-co2-laser-for-the-treatment-of-genitourinary-syndrome-of-menopause-1-year-outcomes
#14
Eric R Sokol, Mickey M Karram
OBJECTIVES: To assess safety and efficacy of a fractional CO2 laser therapy for the treatment of genitourinary syndrome of menopause (GSM) with follow-up to 1 year posttreatment. METHODS: Women presenting with GSM and meeting inclusion criterion were enrolled. Visual Analog Scales were used to grade vaginal pain, burning, itching, dryness, dyspareunia, and dysuria. Dilators were used to rate vaginal elasticity at baseline and at each follow-up visit. Before each treatment and at follow-up, Vaginal Health Index scoring and Female Sexual Function Index questionnaires were completed...
February 6, 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28167201/efficacy-of-iontophoresis-assisted-ablative-fractional-laser-photodynamic-therapy-with-short-incubation-time-for-the-treatment-of-actinic-keratosis-12-month-follow-up-results-of-a-prospective-randomised-comparative-trial
#15
Seung-Hwan Choi, Tae-Hoon Kim, Ki-Hoon Song
BACKGROUND: Iontophoresis is a transdermal drug-delivery technique that enhances the transport of ionic species across membranes and may have significant benefit for the treatment of actinic keratosis (AK) by ablative fractional laser-primed photodynamic therapy (AFL-PDT). The aim of this study were to compare the efficacy, recurrence rate, cosmetic outcome and safety of iontophoresis-assisted AFL-PDT with 2hours of incubation vs. those of conventional AFL-PDT with 2- and 3-hour incubation in patients with facial and scalp AK...
February 3, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28163981/current-advances-in-5-aminolevulinic-acid-mediated-photodynamic-therapy
#16
Connor Thunshelle, Rui Yin, Qiquan Chen, Michael R Hamblin
Kennedy and Pottier discovered that photodynamic therapy (PDT) could be carried out using a procedure consisting of topical application of the porphyrin-precursor, 5-aminolevulinic acid (ALA) to the skin, followed after some time by illumination with various light parameters in the 1980s. Since then, ALA-PDT has expanded enormously and now covers most aspects of dermatological disease. The purpose of this review is to discuss a range of ingenious strategies that investigators have devised for improving the overall outcome (higher efficiency and lower side effects) of ALA-PDT...
September 2016: Current Dermatology Reports
https://www.readbyqxmd.com/read/28163358/mirrored-stainless-steel-substrate-provides-improved-signal-for-raman-spectroscopy-of-tissue-and-cells
#17
Aaran T Lewis, Riana Gaifulina, Martin Isabelle, Jennifer Dorney, Mae L Woods, Gavin R Lloyd, Katherine Lau, Manuel Rodriguez-Justo, Catherine Kendall, Nicholas Stone, Geraint M Thomas
Raman spectroscopy (RS) is a powerful technique that permits the non-destructive chemical analysis of cells and tissues without the need for expensive and complex sample preparation. To date, samples have been routinely mounted onto calcium fluoride (CaF2) as this material possesses the desired mechanical and optical properties for analysis, but CaF2 is both expensive and brittle and this prevents the technique from being routinely adopted. Furthermore, Raman scattering is a weak phenomenon and CaF2 provides no means of increasing signal...
January 2017: Journal of Raman Spectroscopy: JRS
https://www.readbyqxmd.com/read/28162939/fatigue-behaviour-of-niti-shape-memory-alloy-scaffolds-produced-by-slm-a-unit-cell-design-comparison
#18
M Speirs, B Van Hooreweder, J Van Humbeeck, J-P Kruth
Selective laser melting (SLM) is an additive manufacturing technique able to produce complex functional parts via successively melting layers of metal powder. This process grants the freedom to design highly complex scaffold components to allow bone ingrowth and aid mechanical anchorage. This paper investigates the compression fatigue behaviour of three different unit cells (octahedron, cellular gyroid and sheet gyroid) of SLM nitinol scaffolds. It was found that triply periodic minimal surfaces display superior static mechanical properties in comparison to conventional octahedron beam lattice structures at identical volume fractions...
January 12, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28154914/laser-therapy-as-a-treatment-modality-for-genitourinary-syndrome-of-menopause-a-critical-appraisal-of-evidence
#19
Angamuthu Arunkalaivanan, Hervinder Kaur, Oseka Onuma
Interest in laser therapy as a nonhormonal option for the treatment of genitourinary syndrome of menopause (GSM) has increased. We conducted a systematic review of the use of laser therapy for the relief of GSM symptoms. Six electronic databases were searched and conference abstracts were searched manually from the introduction of laser therapy to the present date. The keywords used were: "genitourinary syndrome", "vulvovaginal atrophy", "postmenopausal symptoms", "laser therapy" and "fractional laser treatment"...
February 2, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28151663/time-resolved-laser-fluorescence-spectroscopy-study-of-the-coordination-chemistry-of-a-hydrophilic-chon-1-2-3-triazol-4-yl-pyridine-ligand-with-cm-iii-and-eu-iii
#20
Christoph Wagner, Eros Mossini, Elena Macerata, Mario Mariani, Arturo Arduini, Alessandro Casnati, Andreas Geist, Petra J Panak
The complexation of Cm(III) and Eu(III) with the novel i-SANEX complexing agent 2,6-bis[1-(propan-1-ol)-1,2,3-triazol-4-yl]pyridine (PTD) was studied by time-resolved laser fluorescence spectroscopy (TRLFS). The formation of 1:3, 1:2, and 1:1 metal/ligand complexes was identified upon increasing PTD concentration in 10(-3) mol/L HClO4 and in 0.44 mol/L HNO3 solutions. For all these complexes, stability constants were determined at different acid concentrations. Though under the extraction conditions proposed for an An/Ln separation process, that is, for 0...
February 2, 2017: Inorganic Chemistry
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