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https://www.readbyqxmd.com/read/28931847/excessive-activation-of-tlr4-nf-%C3%AE%C2%BAb-interactively-suppresses-the-canonical-wnt-%C3%AE-catenin-pathway-and-induces-sanfh-in-sd-rats
#1
Junpeng Pei, Lihong Fan, Kai Nan, Jia Li, Zhibin Shi, Xiaoqian Dang, Kunzheng Wang
Nuclear factor-kappa B (NF-κB) interactively affects the Wnt/β-catenin pathway and is closely related to different diseases. However, such crosstalk effect in steroid-associated necrosis of femoral head (SANFH) has not been fully explored and evaluated. In this study, early-stage SANFH was induced by two doses of lipopolysaccharide (LPS, 2 mg/kg/day) and three doses of methylprednisolone (MPS, 40 mg/kg/day). LPS and pyrrolidine dithiocarbamate (PDTC) were administered to activate the TLR4/NF-κB pathway and selectively block the activation of NF-κB, respectively...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931828/a-novel-h2s-releasing-amino-bisphosphonate-which-combines-bone-anti-catabolic-and-anabolic-functions
#2
Simona Rapposelli, Laura Gambari, Maria Digiacomo, Valentina Citi, Gina Lisignoli, Cristina Manferdini, Vincenzo Calderone, Francesco Grassi
Bisphosphonates (BPs) are the first-line treatment of bone loss resulting from various pathological conditions. Due to their high affinity to bone they have been used to develop conjugates with pro-anabolic or anti-catabolic drugs. We recently demontrated that hydrogen sulfide (H2S), promotes osteogenesis and inhibits osteoclast differentiation. Here we developed an innovative molecule, named DM-22, obtained from the combination of alendronate (AL) and the H2S-releasing moiety aryl-isothiocyanate. DM-22 and AL were assayed in vitro in the concentration range 1-33 μM for effects on viability and function of human osteoclasts (h-OCs) and mesenchymal stromal cells (h-MSCs) undergoing osteogenic differentiation...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931598/coordinated-increase-of-nuclear-tension-and-lamin-a-with-matrix-stiffness-out-competes-lamin-b-receptor-which-favors-soft-tissue-phenotypes
#3
Amnon Buxboim, Jerome Irianto, Joe Swift, Avathamsa Athirasala, Jae-Won Shin, Florian Rehfeldt, Dennis E Discher
Matrix stiffness that is sensed by a cell or measured by a purely physical probe reflects the intrinsic elasticity of the matrix and also how thick or thin the matrix is. Here, mesenchymal stem cells (MSCs) and their nuclei spread in response to thickness-corrected matrix micro-elasticity, with increases in nuclear tension and nuclear stiffness resulting from increases in myosin-II and lamin-A,C. Linearity between the widely varying projected area of a cell and its nucleus across many matrices, timescales, and myosin-II activity levels indicates a constant ratio of nucleus-to-cell volume, despite the differentiation potential of MSCs...
September 20, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28930985/visualizing-angiogenesis-by-multiphoton-microscopy-in-vivo-in-genetically-modified-3d-plga-nhap-scaffold-for-calvarial-critical-bone-defect-repair
#4
Jian Li, Holger Jahr, Wei Zheng, Pei-Gen Ren
The reconstruction of critically sized bone defects remains a serious clinical problem because of poor angiogenesis within tissue-engineered scaffolds during repair, which gives rise to a lack of sufficient blood supply and causes necrosis of the new tissues. Rapid vascularization is a vital prerequisite for new tissue survival and integration with existing host tissue. The de novo generation of vasculature in scaffolds is one of the most important steps in making bone regeneration more efficient, allowing repairing tissue to grow into a scaffold...
September 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930929/avoidance-of-tracheostomy-using-mandibular-distraction-in-an-infant-with-severe-condylar-dysplasia-and-airway-obstruction
#5
Anthony J Archual, Jonathan S Black
Craniofacial microsomia (CFM) is a common craniofacial anomaly characterized by asymmetric malformation of the mandible, ear, and other structures including the upper airway. The degree of mandibular hypoplasia is classified using a scheme developed by Pruzansky and modified by Kaban. Severe condylar dysplasia is the hallmark of Pruzansky-Kaban types IIB and III. Distraction osteogenesis has emerged as a treatment modality for mandibular hypoplasia in this setting for the treatment of asymmetry and airway obstruction...
September 19, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28930778/radioprotection-with-amifostine-enhances-bone-strength-and-regeneration-and-bony-union-in-a-rat-model-of-mandibular-distraction-osteogenesis
#6
Catherine N Tchanque-Fossuo, Alexis Donneys, Sagar S Deshpande, Deniz Sarhaddi, Noah S Nelson, Laura A Monson, Sara E Dahle, Steve A Goldstein, Steven R Buchman
BACKGROUND: Using distraction osteogenesis (DO) to regenerate robust endogenous bone could greatly enhance postoncologic reconstruction of head and neck cancer. However, radiation (XRT) corrosive effects still preclude DO's immense potential. We posit that adjunctive pretreatment with the radioprotectant amifostine (AMF) can optimize wound healing and allow for successful DO with quantifiable enhancements in bony union and strength despite previous surgical bed irradiation. METHODS: Two groups of murine left hemimandibles were exposed to a human equivalent radiation dosage fractionated over 5 daily doses of 7 Gy...
September 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28929434/random-electromyostimulation-promotes-osteogenesis-and-the-mechanical-properties-of-rat-bones
#7
Shigeo M Tanaka, Yuma Yorozuya, Daisuke Takatsu
Exercise is often recommended as a promising non-pharmacologic countermeasure to prevent osteoporosis. However, elderly osteoporotic patients generally have physical fitness difficulties preventing them from performing effective and sustainable exercise. Electromyostimulation should be one effective modality for non-pharmacological prevention of osteoporosis without any voluntary physical movements. However, successful stimulation patterns remain controversial. As suggested by our previous in vitro studies, randomized timing of stimulation could be a candidate to maximize the osteogenic effect of electromyostimulation...
September 19, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28926111/regulating-osteogenesis-and-adipogenesis-in-adipose-derived-stem-cells-by-controlling-underlying-substrate-stiffness
#8
Tao Zhang, Shiyu Lin, Xiaoru Shao, Sirong Shi, Qi Zhang, Changyue Xue, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
Cells reside in a complex microenvironment (niche) in which the biochemical and biophysical properties of the extracellular matrix profoundly affect cell behavior. Extracellular stiffness, one important bio-mechanical characteristic of the cell niche, is important in regulating cell proliferation, migration, and lineage specification. However, the mechanism by which mechanical signals guide osteogenic and adipogenic commitment of stem cells remains difficult to dissect. To explore this question, we generated a range of polydimethylsiloxane-based matrices with differing degrees of stiffness that mimicked the stiffness seen in natural tissues and examined adipose stem cell morphology, spreading, vinculin expression and differentiation along the osteogenic and adipogenic pathways...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28925678/dual-functionalized-graphene-oxide-based-sirna-delivery-system-for-implant-surface-bio-modification-with-enhanced-osteogenesis
#9
Li Zhang, Qing Zhou, Wen Song, Kaimin Wu, Yumei Zhang, Yimin Zhao
Surface functionalization by siRNA is a novel strategy for improved implant osseointegration. Gene delivery system with safety and high transfection activity is a crucial factor for siRNA-functionalized implant to exert its biological function. To this end, polyethylene glycol (PEG) and polyethylenimine (PEI) dual-functionalized graphene oxide (GO) (nGO-PEG-PEI) may present a promising siRNA vector. In this study, nano-sized nGO-PEG-PEI was prepared and optimized for siRNA delivery. Titania nanotubes (NT) fabricated by anodic oxidation were bio-modified with nGO-PEG-PEI/siRNA by cathodic electrodeposition, designated as NT-GPP/siRNA...
September 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28924529/anterior-fontanelle-wormian-bone-fontanellar-bone-a-review-of-this-rare-anomaly-with-case-illustration
#10
Jaspreet Johal, Joe Iwanaga, Marios Loukas, R Shane Tubbs
Wormian bones are a relatively rare skeletal anomaly that present as accessory bone(s) within the sutures of the cranium and even more rarely within the fontanelles. It is believed that they arise from the formation of abnormal cranial ossification centers. Although not extensively reported in the literature, this anomaly is thought to be associated with other anatomical anomalies such as osteogenesis imperfecta, rickets, and other bone dysplasias. When located within the fontanelles, the most likely site of occurrence is the posterior fontanelle...
July 7, 2017: Curēus
https://www.readbyqxmd.com/read/28924384/fgfr3-deficient-mice-have-accelerated-fracture-repair
#11
Yangli Xie, Fengtao Luo, Wei Xu, Zuqiang Wang, Xianding Sun, Meng Xu, Junlan Huang, Dali Zhang, Qiaoyan Tan, Bo Chen, Wanling Jiang, Xiaolan Du, Lin Chen
Bone fracture healing is processed through multiple biological stages that partly recapitulates the skeletal development process. FGFR3 is a negative regulator of chondrogenesis during embryonic stage and plays an important role in both chondrogenesis and osteogenesis. We have investigated the role of FGFR3 in fracture healing using unstabilized fracture model and found that gain-of-function mutation of FGFR3 inhibits the initiation of chondrogenesis during cartilage callus formation. Here, we created closed, stabilized proximal tibia fractures with an intramedullary pin in Fgfr3(-/-)mice and their littermate wild-type mice...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28923271/biological-effects-of-orthodontic-tooth-movement-into-the-grafted-alveolar-cleft
#12
Jian Sun, Xiaoyue Zhang, Renmei Li, Zhengxi Chen, Yuanliang Huang, Zhenqi Chen
PURPOSE: Functional stimulus during orthodontic tooth movement into the grafted bone can lead to better alveolar bone grafting outcomes. The aim of this study was to analyze the biological effects of orthodontic tooth movement into the grafted alveolar cleft area with histologic staining, fluorescence staining, and real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: An animal model of orthodontic tooth movement into the grafted alveolar cleft area was established in 8-week-old Sprague-Dawley rats...
August 24, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28921615/plasticizer-di-2-ethylhexyl-phthalate-interferes-with-osteoblastogenesis-and-adipogenesis-in-a-mouse-model
#13
Chen-Yuan Chiu, Shih-Chun Sun, Chih-Kang Chiang, Ching-Chia Wang, Ding-Cheng Chan, Huang-Jen Chen, Shing-Hwa Liu, Rong-Sen Yang
Plasticizer di(2-ethylhexyl)phthalate (DEHP) can leach from medical devices such as blood storage bags and the tubing. Recently, epidemiological studies showed that phthalate metabolites levels in the urine are associated with low bone mineral density (BMD) in older women. The detailed effect and mechanism of DEHP on osteoblastogenesis and adipogenesis and bone loss remain to be clarified. Here, we investigated the effect and mechanism of DEHP and its active metabolite mono(2 -ethylhexyl)phthalate (MEHP) on osteoblastogenesis and adipogenesis...
September 16, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28919414/changes-in-related-circular-rnas-following-er%C3%AE-knockdown-and-the-relationship-to-rbmsc-osteogenesis
#14
Xiaoyun Li, Bojia Peng, Xiaofeng Zhu, Panpan Wang, Yingquan Xiong, Hengrui Liu, Kehuan Sun, Haixia Wang, Ling Ou, Zhidi Wu, Xiaoguang Liu, Haibin He, Shu Mo, Xunqian Peng, Ya Tian, Ronghua Zhang, Li Yang
Recently, several studies have indicated that circular RNAs (circRNAs) play significant roles in various disease; however, Little is known about the chronology of estrogen receptor beta (ERβ) deficiency and altered circRNA expression, or their relationship with osteogenesis. Herein, we show through western-blot and quantitative real-time PCR assays, that when ERβ is silenced, the expression of osteogenesis-related proteins and mRNAs were down-regulated. We then performed RNA-Seq to analyze differential circRNA expression between the control and ERβ knockdown group...
September 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28916840/a-novel-col1a2-c-propeptide-cleavage-site-mutation-causing-high-bone-mass-osteogenesis-imperfecta-with-a-regional-distribution-pattern
#15
T Rolvien, U Kornak, J Stürznickel, T Schinke, M Amling, S Mundlos, R Oheim
Osteogenesis imperfecta (OI) is typically characterized by low bone mass and increased bone fragility caused by heterozygous mutations in the type I procollagen genes (COL1A1/COL1A2). We report two cases of a 56-year-old woman and her 80-year-old mother who suffered from multiple vertebral and non-vertebral fractures with onset in early childhood. A full osteologic assessment including dual-energy X-ray absorptiometry (DXA), high-resolution peripheral quantitative computed tomography (HR-pQCT), and serum analyses pointed to a high bone mineral density (BMD) in the hip (DXA Z-score + 3...
September 15, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28916811/an-enu-induced-splice-site-mutation-of-mouse-col1a1-causing-recessive-osteogenesis-imperfecta-and-revealing-a-novel-splicing-rescue
#16
Koichi Tabeta, Xin Du, Kei Arimatsu, Mai Yokoji, Naoki Takahashi, Norio Amizuka, Tomoka Hasegawa, Karine Crozat, Tomoki Maekawa, Sayuri Miyauchi, Yumi Matsuda, Takako Ida, Masaru Kaku, Kasper Hoebe, Kinji Ohno, Hiromasa Yoshie, Kazuhisa Yamazaki, Eva Marie Y Moresco, Bruce Beutler
GU-AG consensus sequences are used for intron recognition in the majority of cases of pre-mRNA splicing in eukaryotes. Mutations at splice junctions often cause exon skipping, short deletions, or insertions in the mature mRNA, underlying one common molecular mechanism of genetic diseases. Using N-ethyl-N-nitrosourea, a novel recessive mutation named seal was produced, associated with fragile bones and susceptibility to fractures (spine and limbs). A single nucleotide transversion (T → A) at the second position of intron 36 of the Col1a1 gene, encoding the type I collagen, α1 chain, was responsible for the phenotype...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28915498/tantalum-coated-pedicle-screws-enhance-implant-integration
#17
Liang-Yu Shi, An Wang, Fa-Zhi Zang, Jian-Xi Wang, Xian-Wei Pan, Hua-Jiang Chen
Because titanium alloy (Ti) has the natural advantage of a low elastic modulus, it has become the most commonly used material for the manufacturing of pedicle screws. However, its poor shear strength and osteogenic ability are undesirable properties. The superior osteoinductivity demonstrated by tantalum (Ta) in oral and maxillofacial surgery and joint surgery leads us to assume that the tantalum-coated pedicle screws may have better osteogenic properties and bone anchoring strength. To verify this hypothesis, MC3T3-E1 cells and human mesenchymal stem cells (hBMSCs) were seeded on the surface of Ta and Ti disks to compare the effects of two different metals on cell adhesion, proliferation, and differentiation...
September 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28915122/understanding-fetal-factors-that-contribute-to-preterm-birth-sj%C3%A3-gren-larsson-syndrome-as-a-model
#18
Pippa Staps, Marije Hogeveen, Joris Fuijkschot, Joris van Drongelen, Michèl A A P Willemsen
AIM: Preterm birth is the world's leading cause of neonatal death. Unfortunately, the pathophysiology of preterm birth remains poorly understood. Sjögren-Larsson syndrome is a rare, neurometabolic disorder caused by a fatty aldehyde dehydrogenase deficiency. A majority of patients with Sjögren-Larsson syndrome is born preterm. METHODS: Data of all known Dutch patients with Sjögren-Larsson syndrome and all cases reported in literature were analyzed to learn from preterm birth in context of this rare disease...
September 15, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28914836/-preclinical-tests-of-additive-materials-for-personified-endoprostheses-of-hand-joints
#19
G P Kotelnikov, A V Kolsanov, L T Volova, Yu V Ponomareva, A N Nikolayenko, S A Prikhodko, N V Popov, A E Shcherbovskikh
AIM: To evaluate the biocompatibility of additive materials for personified endoprostheses of hand joints in vivo. MATERIAL AND METHODS: We tested a material based on titanium that was implanted into muscles and bone tissue in experiment on rabbits. Follow-up was 30 and 90 days. RESULTS: Implantation into muscle tissue is accompanied by reaction against foreign body followed by fibrosis without concomitant inflammation. Induction of osteogenesis and trabecular structures remodeling were detected after implantation into bone tissue...
2017: Khirurgiia
https://www.readbyqxmd.com/read/28913830/ontogenesis-of-opercular-deformities-in-gilthead-sea-bream-sparus-aurata-a-histological-description
#20
N P Thuong, B Verstraeten, B D Kegel, J Christiaens, T D Wolf, P Sorgeloos, D Bonte, D Adriaens
The aim of this study was to characterize histological changes during opercular osteogenesis in farmed gilthead sea bream Sparus aurata larvae from 7 to 69 days post hatching (dph) and compare normal osteogenesis with that of deformed opercles. Mild opercular deformities were first detected in 19 dph larvae by folding of the opercle's distal edge into the gill chamber. Here, the variation in the phenotype and the irregular bone structure at the curled part of the opercles is described and compared with the histology of normal opercles...
September 15, 2017: Journal of Fish Biology
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