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https://www.readbyqxmd.com/read/28093323/in-vitro-gastric-survival-of-commercially-available-probiotic-strains-and-oral-dosage-forms
#1
Romain Caillard, Nicolas Lapointe
Although the intestinal microbial community is still incompletely understood, there is strong evidence of the benefits of using probiotics to address some medical states or conditions. As a result, the probiotics oral supplements market has exploded during the last few years. However, while their sensitivity to gastric juices, acidic pH and bile is well known, most of these oral forms would not guarantee any survival of the strains in such conditions. In this work, we have studied the resistance to simulated gastric juices of several commercially available probiotics products...
January 13, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28081355/oral-delivery-of-probiotics-in-poultry-using-ph-sensitive-tablets
#2
Tao Jiang, Hui-Shan Li, Geon Goo Han, Bijay Singh, Sang-Kee Kang, Jin-Duck Bok, Dae-Duk Kim, Zhong-Shan Hong, Yun-Jaie Choi, Chong-Su Cho
As alternatives to antibiotics in live stocks, probiotics have been used although most of them in the form of liquid or semisolid formulations which show low cell viability after oral administration. Therefore, suitable dry dosage forms should be developed for live stocks to protect probiotics against the low pH in the stomach such that the products have higher probiotics survivability. Here, in order to develop a dry dosage forms of probiotics for poultry, we used hydroxyproyl methylcellulose phthalate 55 (HPMCP 55) as a tablet forming matrix to develop probiotics in a tablet form for poultry...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28067304/weissella-cibaria-wikim28-ameliorates-atopic-dermatitis-like-skin-lesions-by-inducing-tolerogenic-dendritic-cells-and-regulatory-t-cells-in-balb-c-mice
#3
Seul Ki Lim, Min-Sung Kwon, Jieun Lee, Young Joon Oh, Ja-Young Jang, Jong-Hee Lee, Hae Woong Park, Young-Do Nam, Myung-Ji Seo, Seong Woon Roh, Hak-Jong Choi
The occurrence of atopic dermatitis (AD), a chronic inflammatory skin disease, has been increasing steadily in children and adults in recent decades. In this study, we evaluated the ability of the lactic acid bacterium Weissella cibaria WIKIM28 isolated from gatkimchi, a Korean fermented vegetable preparation made from mustard leaves, to suppress the development of AD induced by 2,4-dinitrochlorobenzene in a murine model. Oral administration of W. cibaria WIKIM28 reduced AD-like skin lesions, epidermal thickening, and serum immunoglobulin E levels...
January 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28065340/enhancing-allergen-presentation-platforms-for-sublingual-immunotherapy
#4
REVIEW
P Moingeon, V Lombardi, V Baron-Bodo, L Mascarell
Sublingual immunotherapy (SLIT) relies on high doses of allergens to treat patients with type I allergies. Although SLIT is commonly performed without any adjuvant or delivery system, allergen(s) could be further formulated with allergen-presentation platforms to better target oral dendritic cells eliciting regulatory immune responses. Improving the availability of allergens to the immune system should enhance SLIT efficacy, while allowing to decrease allergen dosing. Herein, we present an overview of adjuvants and vector systems that have been, or could be, considered as candidate allergen-presentation platforms for the sublingual route...
January 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28063305/inhibitory-effects-of-lactobacillus-rhamnosus-and-lactobacillus-casei-on-candida-biofilm-of-denture-surface
#5
Young-Gyun Song, Sung-Hoon Lee
OBJECTIVE: Candida albicans biofilm is associated with denture-related stomatitis and oral candidiasis of elderly. Probiotics are beneficial bacteria and have antibacterial activity against pathogenic bacteria. The purpose of this study was to investigate the antifungal activity of various probiotics against C. albicans and the inhibitory effects of probiotics on Candida biofilm on the denture surface. DESIGN: The spent culture media of various probiotics were investigated the antifungal efficacy against C...
December 31, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28057659/lactobacillus-reuteri-dsm-17938-in-the-prevention-of-antibiotic-associated-diarrhoea-in-children-protocol-of-a-randomised-controlled-trial
#6
Maciej Kołodziej, Hania Szajewska
INTRODUCTION: Administration of some probiotics appears to reduce the risk of antibiotic-associated diarrhoea (AAD). The effects of probiotics are strain-specific, thus, the efficacy and safety of each probiotic strain should be established separately. We aim to assess the effects of Lactobacillus reuteri DSM 17938 administration for the prevention of diarrhoea and AAD in children. METHODS AND ANALYSIS: A total of 250 children younger than 18 years treated with antibiotics will be enrolled in a double-blind, randomised, placebo-controlled trial in which they will additionally receive L...
January 5, 2017: BMJ Open
https://www.readbyqxmd.com/read/28050461/role-of-saccharomyces-boulardii-in-reduction-of-neonatal-hyperbilirubinemia
#7
V Suganthi, A Gokul Das
INTRODUCTION: Probiotics are known to reduce the severity of hyperbilirubinemia. AIM: This study was done to evaluate the effect of probiotic on neonatal hyperbilirubinemia in term neonates. MATERIALS AND METHODS: A total of 181 healthy term neonates after birth were divided into a control group (n=95) and a treatment group (n=86) randomly and treated with placebo and probiotic (Saccharomyces boulardii) respectively. A total of two doses were given orally in the first two consecutive days...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28045137/the-effects-of-lactobacillus-plantarum-on-small-intestinal-barrier-function-and-mucosal-gene-transcription-a-randomized-double-blind-placebo-controlled-trial
#8
Zlatan Mujagic, Paul de Vos, Mark V Boekschoten, Coen Govers, Harm-Jan H M Pieters, Nicole J W de Wit, Peter A Bron, Ad A M Masclee, Freddy J Troost
The aim of this study was to investigate the effects of three Lactobacillus plantarum strains on in-vivo small intestinal barrier function and gut mucosal gene transcription in human subjects. The strains were selected for their differential effects on TLR signalling and tight junction protein rearrangement, which may lead to beneficial effects in a stressed human gut mucosa. Ten healthy volunteers participated in four different intervention periods: 7-day oral intake of either L. plantarum WCFS1, CIP104448, TIFN101 or placebo, proceeded by a 4 weeks wash-out period...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043872/extensively-hydrolysed-casein-formula-containing-l-rhamnosus-gg-reduces-the-occurrence-of-other-allergic-manifestations-in-children-with-cow-s-milk-allergy-3-year-randomized-controlled-trial
#9
Roberto Berni Canani, Margherita Di Costanzo, Giorgio Bedogni, Antonio Amoroso, Linda Cosenza, Carmen Di Scala, Viviana Granata, Rita Nocerino
BACKGROUND: Children with cow's milk allergy (CMA) have an increased risk of developing other allergic manifestations (AMs). OBJECTIVE: We performed a parallel-arm randomized controlled trial (RCT) to test whether the administration of an extensively hydrolysed casein formula (EHCF) containing the probiotic L. rhamnosus GG (LGG) can reduce the occurrence of other AMs in CMA children. METHODS: Children with IgE-mediated CMA were randomly allocated to EHCF or EHCF+LGG groups and followed for 36 months...
December 30, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28042275/the-level-of-beta-defensin-2-in-saliva-and-its-expression-in-parotid-gland-epithelial-cells-after-probiotic-lactobacillus-reuteri-induction-to-inhibit-streptococcus-mutans-in-caries
#10
Tuti Kusumaningsih, M S Subijanto, Retno Indrawati, R Rini Devijanti
OBJECTIVE: The aim of this study was to prove that administrating L. reuteri probiotics can increase the level of BD-2 saliva and BD-2 expression in the epithelial parotid glands of Wistar rats. MATERIALS AND METHODS: Experimental design in this study was randomized control group post test only. Twenty-four white male Rattus norvegicus Wistar strain rats were divided into four groups. The negative control group included rats not induced by S. mutans whereas the positive control group included rats induced by S...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28041735/beneficial-effects-of-probiotic-cholesterol-lowering-strain-of-enterococcus-faecium-wefa23-from-infants-on-diet-induced-metabolic-syndrome-in-rats
#11
Fen Zhang, Liang Qiu, Xiongpeng Xu, Zhengqi Liu, Hui Zhan, Xueying Tao, Nagendra P Shah, Hua Wei
The aim of this study was to select probiotic Enterococcus strains which have the potential to improve metabolic syndrome (MS). Ten Enterococcus strains isolated from healthy infants were evaluated for their probiotic properties in vitro, and Enterococcus faecium WEFA23 was selected due to its cholesterol removal ability (1.89 ± 0.07 mg/10(10) cfu), highest glycodeoxycholic acid-hydrolase activity (1.86 ± 0.01 U/mg), and strong adhesion capacity to Caco-2 cells (17.90 ± 0.19%). The safety of E. faecium WEFA23 was verified by acute oral administration in mice, and it was found to have no adverse effects on general health status, bacterial translocation, and gut mucosal histology...
December 29, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/28034835/comparative-evaluation-of-dietary-probiotics-bacillus-subtilis-wb60-and-lactobacillus-plantarum-kctc3928-on-the-growth-performance-immunological-parameters-gut-morphology-and-disease-resistance-in-japanese-eel-anguilla-japonica
#12
Seunghan Lee, Kumar Katya, Youngjin Park, Seonghun Won, Minji Seong, Ali Hamidoghli, Sungchul C Bai
The current experiment was conducted to evaluate and compare the efficacy of two different probiotics Bacillus subtilis WB60 and Lactobacillus plantarum KCTC3928 in diet of Japanese eel, Anguilla japonica. Seven experimental diets were formulated to contain no probiotics (CON), three graded levels of B. subtilis at 10(6) (BS1), 10(7) (BS2), 10(8) (BS3) and L. plantarum at 10(6) (LP1), 10(7) (LP2), 10(8) (LP3) CFU/g diet. Twenty fish averaging 8.29 ± 0.06 g were distributed in to 21 aquaria and were randomly assigned to one of the experimental diets in triplicate groups...
December 26, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28034294/probiotic-properties-of-lactobacillus-fermentum-strains-isolated-from-human-oral-samples-and-description-of-their-antibacterial-activity
#13
Virginia Fuochia, Giovanni Li Volti, Pio Maria Furneri
BACKGROUND: Gram positive bacteria produce peptides, defined bacteriocins which exhibit good antibacterial activity. OBJECTIVE: We evaluated the ability of L. fermentum to produce bacteriocins having therefore, good probiotic features and finally, be safe towards microglial cells. METHOD: Eight wild strains, identified using molecular techniques, were investigated for the evaluation of resistance to bile salts, low pH, H2O2 production, biofilm formation, antibacterial activity and safety on microglia cells (BV2)...
December 29, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28029408/gut-microbiota-and-allergy-asthma-from-pathogenesis-to-new-therapeutic-strategies
#14
REVIEW
Y B Kang, Y Cai, H Zhang
Asthma and atopy, classically associated with hyper-activation of the T helper 2 (Th2) arm of adaptive immunity, are among the most common chronic illnesses worldwide. Emerging evidence relates atopy and asthma to the composition and function of gut microbiota composition. Moreover, certain gut microbial strains have been shown to inhibit or attenuate immune responses associated with chronic inflammation in experimental models. Although still a relatively nascent field of research, evidence to date suggests that the gut microbiome may represent fertile targets for prevention or management of allergic asthma and other diseases in which adaptive immune dysfunction is a prominent feature...
October 28, 2016: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28029082/probiotics-in-curing-allergic-and-inflammatory-conditions-research-progress-and-futuristic-vision
#15
Kuldeep Dhama, Shyma K Latheef, Ashok Kumar Munjal, Rekha Khandia, Hari Abdul Samad, Hafiz M N Iqbal, Sunil K Joshi
BACKGROUND: Probiotics constitute the viable and beneficial microbes, which offer a dietary means to sustain the balance of gastro-intestinal (GI) microflora. Owing to their multiple health benefits, these have recently gained wide attention among researchers for exploring their potential in safeguarding the health of humans and animals. Probiotics could also modulate host-immune responses, thereby help in counteracting the immunological dysfunctions. Probiotics can inhibit the systemic invasion of pathogens entering through the GI mucosa/ oral cavity and have been found to possess effective prophylactic and therapeutic utilities against various infectious pathogens as well as non-infectious diseases and disorders...
December 26, 2016: Recent Patents on Inflammation & Allergy Drug Discovery
https://www.readbyqxmd.com/read/28028690/effect-of-lactobacillus-reuteri-on-cell-viability-and-pge2-production-in-human-gingival-fibroblasts
#16
Gina A Castiblanco, Tulay Yucel-Lindberg, Stefan Roos, Svante Twetman
Emerging evidence suggests that probiotic therapy can play a role in the prevention and management of oral inflammatory diseases through immunomodulation and down-regulation of the inflammatory cascade. The aim of this in vitro study was to investigate the viability of human gingival fibroblasts (HGF) and its production of prostaglandin E2 (PGE2), when exposed to supernatants of two mixed Lactobacillus reuteri strains (ATCC PTA 5298 and DSM 17938). The experiments were conducted in the presence and absence of the pro-inflammatory cytokine IL-1β...
December 27, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28025548/improvement-of-intestinal-immune-cell-function-by-lactic-acid-bacteria-for-dairy-products
#17
Tomonori Kamiya, Yohei Watanabe, Seiya Makino, Hiroshi Kano, Noriko M Tsuji
Lactic acid bacteria (LAB) form a major component of gut microbiota and are often used as probiotics for fermented foods, such as yoghurt. In this study, we aimed to evaluate immunomodulatory activity of LAB, especially that of Lactobacillus bulgaricus ME-552 (ME552) and Streptococcus thermophilus ME-553 (ME553). In vivo/in vitro assay was performed in order to investigate their effects on T cell function. After oral administration of ME553 to C57BL/6 mice, the amount of both interferon γ (IFN-γ) and interleukin 17 (IL-17) produced by cluster of differentiation (CD) 4⁺ T cells from Peyer's patches (PPs) were significantly enhanced...
December 23, 2016: Microorganisms
https://www.readbyqxmd.com/read/28018362/visualization-of-probiotic-mediated-ca-2-signaling-in-intestinal-epithelial-cells-in-vivo
#18
Takahiro Adachi, Shigeru Kakuta, Yoshiko Aihara, Tomonori Kamiya, Yohei Watanabe, Naomi Osakabe, Naoki Hazato, Atsushi Miyawaki, Soichiro Yoshikawa, Takako Usami, Hajime Karasuyama, Hiromi Kimoto-Nira, Kazuhiro Hirayama, Noriko M Tsuji
Probiotics, such as lactic acid bacteria (LAB) and Bacillus subtilis var. natto, have been shown to modulate immune responses. It is important to understand how probiotic bacteria impact intestinal epithelial cells (IECs), because IECs are the first line of defense at the mucosal surface barrier and their activities substantially affect the gut microenvironment and immunity. However, to date, their precise mechanism remains unknown due to a lack of analytical systems available for live animal models. Recently, we generated a conditional Ca(2+) biosensor Yellow Cameleon (YC3...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/28012076/erratum-to-orally-administered-multispecies-probiotic-formulations-to-prevent-uro-genital-infections-a-randomized-placebo-controlled-pilot-study
#19
Valerio Mezzasalma, Enrico Manfrini, Emanuele Ferri, Marco Boccarusso, Patrizia Di Gennaro, Irene Schiano, Angela Michelotti, Massimo Labra
No abstract text is available yet for this article.
December 23, 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28009106/metabolic-effects-of-lactobacillus-reuteri-dsm-17938-in-patients-with-type-2-diabetes-a-randomized-controlled-trial
#20
Reza Mobini, Valentina Tremaroli, Marcus Ståhlman, Fredrik Karlsson, Max Levin, Maria Ljungberg, Maja Sohlin, Heléne Bertéus Forslund, Rosie Perkins, Fredrik Bäckhed, Per-Anders Jansson
AIMS: To investigate the metabolic effects of 12-week oral supplementation with Lactobacillus reuteri DSM 17938 in patients with type 2 diabetes on insulin therapy. MATERIALS AND METHODS: In a double-blind trial, we randomized 46 patients with type 2 diabetes to placebo or a low (10(8) CFU/day) or high dose (10(10) CFU/day) of L. reuteri DSM 17938 for 12 weeks. The primary endpoint was the effect of supplementation on glycated hemoglobin (HbA1c). Secondary endpoints were insulin sensitivity (assessed by glucose clamp), liver fat content, body composition, body fat distribution, fecal microbiota composition and serum bile acids...
December 23, 2016: Diabetes, Obesity & Metabolism
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