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Shanmugasundaram Udhayavel, Gopalakrishnamurthy Thippichettypalayam Ramasamy, Vasudevan Gowthaman, Shanmugasamy Malmarugan, Kandasamy Senthilvel
This work has been undertaken to study the occurrence of Clostridium perfringens contamination in the poultry feed ingredients and find out its in-vitro antibiotic sensitivity pattern to various antimicrobial drugs. Two hundred and ninety-eight poultry feed ingredient samples received at Poultry Disease Diagnosis and Surveillance Laboratory, Namakkal, Tamil Nadu in South India were screened for the presence of C. perfringens . The organisms were isolated in Perfringens agar under anaerobic condition and subjected to standard biochemical tests for confirmation...
September 2017: Animal nutrition
Abdelfattah Z M Salem, Ameer Khusro, Mona M Y Elghandour, Jaime Olivares-Pérez, Saúl Rojas-Hernandez, Régulo Jiménez-Guillén
The pivotal aim of the present context was to isolate diversified group of bacteria from the ruminants and to evaluate their antibiogram pattern against 22 antibiotics of 14 different classes. The bacterial isolates from small and large ruminant (sheep, cattle, and calves) were isolated from the rumen based on various colonies morphology, and subjected for preliminary antibiotics susceptibility assay using disc diffusion method. The most sensitive isolates (based on zone of inhibition) were selected for determining the minimum inhibitory concentration (MIC) of each antibiotic ranging from 1 to 256 μg/mL...
May 12, 2018: Microbial Pathogenesis
Stella Y Chung, Leon Rafailov, Roger E Turbin, Paul D Langer
PURPOSE: Recent studies suggest an increasing incidence of gram-negative bacteria and methicillin-resistant Staphylococcus aureus in dacryocystitis. Since patients are often treated empirically without culture data, a changing microbiologic profile will markedly affect the success of oral treatment. To provide current guidelines for the treatment of this common condition, we investigated the microbiology and antibiogram of dacryocystitis seen at our institution. METHODS: The charts of all patients presenting with acute and/or chronic dacryocystitis in University Hospital, Newark, from 2007 to 2015 were reviewed...
May 11, 2018: Orbit
Chan Liu, Juan Feng, Defeng Zhang, Yundan Xie, Anxing Li, Jiangyong Wang, Youlu Su
In view of the changing antibiotic-resistance profiles of Streptococcus agalactiae from tilapia in China, antimicrobial susceptibilities of 75 S. agalactiae strains were determined by the disc diffusion method, and cluster analyses of the antibiograms and antibiogram types were performed. All strains displayed multidrug resistance (MDR). The antimicrobial-resistance rates were highest (>90%) to aminoglycosides, sulfonamides, pipemidic acid, and norfloxacin, followed by penicillin, ampicillin, and ciprofloxacin (26...
May 11, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
M Mahendra, B S Jayaraj, K S Lokesh, S K Chaya, Vivek Vardhan Veerapaneni, Sneha Limaye, Raja Dhar, Rajesh Swarnakar, Shrikant Ambalkar, P A Mahesh
Aim of Study: Respiratory infections account for significant morbidity, mortality and expenses to patients getting admitted to ICU. Antibiotic resistance is a major worldwide concern in ICU, including India. It is important to know the antibiotic prescribing pattern in ICU, organisms and its resistance pattern as there is sparse data on Indian ICUs. Materials and Methods: We conducted a prospective study from August 2015 to February 2016. All patients getting admitted to RICU with respiratory infection who were treated with antibiotics were included into study...
April 2018: Indian Journal of Critical Care Medicine
Patrícia Giovana Hoepers, Paulo Lourenço Silva, Daise Aparecida Rossi, Edson Campos Valadares Júnior, Bruna Custódio Ferreira, João Paulo Zuffo, Priscilla Karina Koerich, Belchiolina Beatriz Fonseca
ABSTRACT- 1. The aim of this study was to analyse the association between Escherichia coli isolates recovered from turkeys and the expression of beta-lactamase genes, such as extended spectrum beta lactamase (ESBL) and ampicillin class C (AmpC). The phenotype of the resistance profile was examined using the association between amoxicillin and ceftiofur resistance. 2. Results showed that 84% from the turkey isolates harboured four or five genes associated with the CoIV plasmid. In an antibiogram test, 82% of the isolates were multi-drug resistant (MDR), the highest levels of resistance being against erythromycin (99%) and amoxicillin (76...
May 8, 2018: British Poultry Science
Hadis Abbasi, Reza Ranjbar
Introduction: Escherichia coli (E.coli) as an opportunistic pathogen is a major cause of the hospital infections. The main goal of this research was to determine the frequency of quinolone resistance genes (qnr) among E.coli pathotypes isolated from patients with urinary tract infections (UTIs). Material and methods: Urine samples were obtained from patients with UTIs in three major hospitals of Mofid, Bu Ali, and Vali-Asr during the year of 2015 in Tehran, Iran...
2018: Central European Journal of Urology
Lok Bahadur Shrestha, Narayan Raj Bhattarai, Basudha Khanal
Introduction: Coagulase-negative staphylococci (CNS) are normal commensals of human skin and mucous membranes. The objective of the study was to determine the prevalence of CNS among clinical isolates, characterize them up to species level, compare the three conventional methods for detection of biofilm formation, and study their antimicrobial susceptibility pattern. Materials and methods: CNS were obtained from various clinical samples including blood, urine, central venous catheter tips, endotracheal tube aspirate, and pus during a 1-year period (July 1, 2014, to June 30, 2015)...
2018: Infection and Drug Resistance
Siri Tribler, Christopher F Brandt, Kristian A Fuglsang, Michael Staun, Per Broebech, Claus E Moser, Thomas Scheike, Palle B Jeppesen
Background: In intestinal failure (IF) patients receiving home parenteral support (HPS), catheter-related bloodstream infections (CRBSIs) frequently result in replacement of their tunneled central venous catheters (CVCs), which may lead to future loss of central venous access. Objective: This observational study investigated the consequences of a catheter-salvage strategy related to CRBSIs. Design: All CRBSIs from 2002 to 2016 in the Copenhagen IF and microbiological databases were retrospectively analyzed...
May 1, 2018: American Journal of Clinical Nutrition
Rakshya Nepal, Basudha Shrestha, Deepak Man Joshi, Rajesh Dhoj Joshi, Sanjit Shrestha, Anjana Singh
BACKGROUND: Lower respiratory tract infection is a common cause of morbidity and mortality worldwide. A cross-sectional study was carried out with an objective to study the antibiogram of Gram-negative isolates of patients with lower respiratory tract infection visiting Kathmandu Model Hospital. METHODS: A total of 274 specimens including sputum, endotracheal aspirates, suction tips were cultured as per standard microbiological technique. Antibiotic susceptibility and detection of Extended-spectrum beta- lactamases (ESBLs) were performed following Clinical Laboratory Standard Institute (CLSI 2014) guidelines...
March 13, 2018: Journal of Nepal Health Research Council
Shree Dhotre, Vilas Jahagirdar, Namdev Suryawanshi, Milind Davane, Ramesh Patil, Basavraj Nagoba
OBJECTIVES: To evaluate the role of periodontitis in viridans group streptococci (VGS) bacteremia and infective endocarditis (IE). METHODS: A total of 200 subjects including two groups. Group A- 34 subjects undergoing tooth extraction with periodontitis, 46 subjects undergoing tooth extraction without periodontitis and 40 healthy controls. Group B: 40 confirmed cases of IE (17 with and 23 without periodontitis) and 40 healthy controls. Subgingival plaque and blood samples were obtained and processed by standard procedures...
March 2018: Indian Heart Journal
D R MacFadden, B Coburn, N Shah, A Robicsek, R Savage, M Elligsen, N Daneman
OBJECTIVES: Early empiric antibiotic therapy in patients can improve clinical outcomes in Gram-negative bacteraemia. However, the widespread prevalence of antibiotic-resistant pathogens compromises our ability to provide adequate therapy while minimizing use of broad antibiotics. We sought to determine whether readily available electronic medical record data could be used to develop predictive models for decision support in Gram-negative bacteraemia. METHODS: We performed a multi-centre cohort study, in Canada and the USA, of hospitalized patients with Gram-negative bloodstream infection from April 2010 to March 2015...
April 25, 2018: Clinical Microbiology and Infection
José Carlos Almansa Ruiz, Annelize Jonker, Anna-Mari Bosman, Gerhard Steenkamp
Twenty-seven microbiological samples were taken from root canals (RC) of the canine teeth of 20 dogs where the pulps were non-vital and exposed due to complicated crown fractures. These pulps were cultured for aerobic/anaerobic bacteria. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer diffusion test. A total of 49 cultivable isolates, belonging to 27 different microbial species and 18 different genera, were recovered from the 27 RCs sampled. Twenty (40.81 per cent) of the cultivable isolates were Gram positive while 29 (59...
April 27, 2018: Veterinary Record
M De Frutos, L López-Urrutia, R Aragón, A M Vegas, M Vázquez, J M Eiros Bouza
OBJECTIVE: Turicella otitidis is a gram-positive bacillus coryneforme described, after 16S RNA sequencing, in 1994 by Funke et al as a microorganism involved in otitis media, and it is in health conditions a habitual colonizer of the external auditory canal. Since its description, more than twenty years ago, few cases of otitis related to or directly attributed to this microorganism have been published. METHODS: Description of a case of mastoiditis that required surgery and other cases of otic pathology in which T...
April 26, 2018: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
Christopher J Hostler, Rebekah W Moehring, Elizabeth S Dodds Ashley, Melissa Johnson, Angelina Davis, Sarah S Lewis, Daniel J Sexton, Deverick J Anderson
OBJECTIVETo determine the feasibility and value of developing a regional antibiogram for community hospitals.DESIGNMulticenter retrospective analysis of antibiograms.SETTING AND PARTICIPANTSA total of 20 community hospitals in central and eastern North Carolina and south central Virginia participated in this study.METHODSWe combined antibiogram data from participating hospitals for 13 clinically relevant gram-negative pathogen-antibiotic combinations. From this combined antibiogram, we developed a regional antibiogram based on the mean susceptibilities of the combined data...
June 2018: Infection Control and Hospital Epidemiology
C Hughes, C Ashhurst-Smith, J K Ferguson
Gram negative anaerobic organisms are important pathogens in a range of clinical infections, and susceptibility testing is not commonly performed in the microbiology laboratory. We performed anaerobic susceptibility testing on 70 clinically relevant Gram negative anaerobes isolated from routine cultures in a busy diagnostic laboratory which were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The susceptibility testing was performed by two methods: Sensititre trays (ThermoFisher Scientific) against 15 different antibiotics, and Etests (bioMérieux) against five clinically relevant antibiotics (metronidazole, piperacillin-tazobactam, amoxicillin-clavulanate, meropenem and clindamycin)...
April 17, 2018: Pathology
Amr Fadel, Mahmoud Mabrok, Salah Aly
The present study was investigating the clinical pictures, prevalence, as well as the ecological conditions associated with Pseudomonas anguilliseptica outbreaks in four cultured seabream (Sparus aurata) farms at different localities in Egypt during the winter season of 2016. The phenotypic and genotypic patterns of pseudomonas isolates were investigated. The existence of intraspecific heterogeneity among different isolates was analyzed using Restriction fragment length polymorphism (RFLP) technique. Attempts on disease control using antibiogram or dietary supplement were also considered...
April 16, 2018: Microbial Pathogenesis
Nitin Gupta, Manish Soneja, Yogiraj Ray, Monalisa Sahu, Kutty Sharada Vinod, Arti Kapil, Ashutosh Biswas, Naveet Wig, Rita Sood
The clinical practice guidelines on nosocomial pneumonia recommends an empirical regimen that would work in 95% of the patients based on the local antibiogram. The aim of the study was development of an antibiogram for guiding empiric therapy in settings with high prevalence of multi-drug resistant organisms. A retrospective review of electronic health records (e-hospital portal) was done to analyze all respiratory isolates from patients admitted in medical wards and intensive care unit between May 2016 and May 2017...
April 17, 2018: Drug Discoveries & Therapeutics
Samar S Boswihi, Edet E Udo, Stefan Monecke, Bindu Mathew, Bobby Noronha, Tina Verghese, Sajida B Tappa
BACKGROUND: Frequent changes in the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) occurring worldwide demand regular surveillance to study their composition and distribution in healthcare facilities. We investigated the genotypic characteristics of MRSA obtained in Kuwait hospitals to better understand their clonal distribution. MATERIALS AND METHODS: A total of 1,327 MRSA isolates obtained from clinical samples in 13 Kuwait hospitals from 1 January to 31 December 2016 were investigated using antibiogram, SCCmec typing, spa typing and DNA microarray...
2018: PloS One
Matthew Song, Thomas J Dilworth, Erik Munson, Jim Davis, Ramy H Elshaboury
Purpose: To determine the frequency at which fluoroquinolones and aminoglycosides demonstrate in vitro activity against non-urinary, non-skin/skin structure Pseudomonas aeruginosa isolates exhibiting decreased susceptibilities to one or more β-lactam agents. Methods: β-lactam-non-susceptible P. aeruginosa isolates recovered from blood, bone, lower respiratory tract, pleural fluid, cerebrospinal fluid, or peritoneal fluid cultures between October 2010 and October 2014 were reviewed from four community hospitals within a single health-system...
November 2017: Therapeutic Advances in Infectious Disease
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