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https://www.readbyqxmd.com/read/29784165/association-between-positive-corneal-rim-cultures-and-microbiology-screening-protocols-in-ontario
#1
Rahul A Sharma, John S Y Park, Yao Wang, Tinghua Zhang, Linda Sharpen, William Dixon, Rookaya Mather
OBJECTIVE: (i) To assess the rate of positive microbiological cultures of corneas prepared by the Eye Bank of Canada (Ontario Division) between January 1, 2012, and December 31, 2013; (ii) to review the microbiology protocols at the 5 major transplant centres in Ontario; and (iii) to assess the incidence of endophthalmitis during the study period. DESIGN: Retrospective chart review. PARTICIPANTS: A total of 4186 consecutive cultured corneal tissues prepared by the Eye Bank from January 1, 2012, to December 31, 2013...
June 2018: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/29757853/multiplex-polymerase-chain-reaction-assay-for-screening-of-mycotoxin-genes-from-ocular-isolates-of-fusarium-species
#2
Orapin Anutarapongpan, Jorge Maestre-Mesa, Eduardo C Alfonso, Terrence P OʼBrien, Darlene Miller
PURPOSE: To identify mycotoxin genes among clinical ocular isolates of Fusarium species and to correlate these with clinical outcomes of Fusarium keratitis. METHODS: Fifty-four clinical isolates of Fusarium were retrieved from the Bascom Palmer Eye Institute Ocular Microbiology Laboratory data bank. Multiplex polymerase chain reactions were run to confirm the identification of Fusarium species [internal transcribed spacer sequence, translation elongation factor 1-α (TEF) and β-tubulin] and to detect the presence of genes encoding production of fumonisin B mycotoxins (FUM1 and FUM8) and trichothecene mycotoxins (deoxynivalenol and nivalenol)...
May 1, 2018: Cornea
https://www.readbyqxmd.com/read/29746818/lymphatic-markers-in-the-human-optic-nerve
#3
A Trost, C Runge, D Bruckner, A Kaser-Eichberger, B Bogner, C Strohmaier, H A Reitsamer, F Schroedl
Tissues of the central nervous system (CNS), including the optic nerve (ON), are considered a-lymphatic. However, lymphatic structures have been described in the dura mater of human ON sheaths. Since it is known that lymphatic markers are also expressed by single non-lymphatic cells, these results need confirmation according to the consensus statement for the use of lymphatic markers in ophthalmologic research. The aim of this study was to screen for the presence of lymphatic structures in the adult human ON using a combination of four lymphatic markers...
May 7, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29746333/effect-of-diabetes-mellitus-and-metformin-on-central-corneal-endothelial-cell-density-in-eye-bank-eyes
#4
Isaac M Chocron, Dinesh K Rai, Ji-Won Kwon, Nicole Bernstein, Jimmy Hu, Moonseong Heo, Jimmy K Lee, Patrick K Gore, Mitchell D McCartney, Roy S Chuck
PURPOSE: To determine whether metformin use and diabetes mellitus (DM) affect central corneal endothelial cell density (ECD) by examining an eye bank corneal donor database. METHODS: The Lions Eye Institute corneal donor database, which consists of 38,318 corneal samples, was examined. Associations of ECD with metformin use and DM were tested by mixed effects linear models that account for correlations of outcomes between eyes within subjects adjusting for age, intraocular lens status, and glaucoma...
May 4, 2018: Cornea
https://www.readbyqxmd.com/read/29746331/is-this-the-future-of-eye-banking
#5
Mark J Mannis, Joel Sugar
No abstract text is available yet for this article.
May 8, 2018: Cornea
https://www.readbyqxmd.com/read/29746326/donor-cornea-harvest-techniques-comparison-between-globe-enucleation-and-in-situ-corneoscleral-disc-excision
#6
Filip Filev, Eileen Bigdon, Nils Alexander Steinhorst, A Kammal, Carolin Schröder, Birgit Wulff, Stephan Linke, Jana Feuerstacke, Olaf Hellwinkel
PURPOSE: To compare whole eye enucleation and corneoscleral disc (CD) excision as donor cornea harvesting techniques for possible effects on corneal cultivation and the clinical outcome of the corneal grafts after transplantation in 2929 cases. METHODS: A retrospective analysis was performed on the Hamburg Eye Bank database using comparative statistics. The standard method for donor cornea recovery at the Hamburg Eye Bank changed from enucleation of the whole eye to CD in situ excision in 2008...
May 8, 2018: Cornea
https://www.readbyqxmd.com/read/29737973/in-vivo-endothelial-cell-density-decline-in-the-early-postoperative-phase-after-descemet-membrane-endothelial-keratoplasty
#7
Alina Miron, Marieke Bruinsma, Lisanne Ham, Sontje-Chiao Schaal, Lamis Baydoun, Isabel Dapena, Silke Oellerich, Gerrit R J Melles
PURPOSE: To evaluate endothelial cell density (ECD) in the first 6 months after Descemet membrane endothelial keratoplasty (DMEK) by eliminating method error as a confounding variable. METHODS: From 24 DMEK eyes operated for Fuchs endothelial corneal dystrophy, from which specular microscopy images could be taken at 1 day and 6 months postoperatively, ECD values were compared between these 2 time points. RESULTS: Using the 1-day ECD measurement as baseline, mean ECD decreased from 1913 (±326) cells/mm to 1524 (±393) cells/mm at 6 months, a decline of -18 (±19)%...
June 2018: Cornea
https://www.readbyqxmd.com/read/29715510/hierarchical-organization-within-the-ventral-premotor-cortex-of-the-macaque-monkey
#8
REVIEW
Kiyoshi Kurata
Recent studies have revealed that the ventral premotor cortex (PMv) of nonhuman primates plays a pivotal role in various behaviors that require the transformation of sensory cues to appropriate actions. Examples include decision-making based on various sensory cues, preparation for upcoming motor behavior, adaptive sensorimotor transformation, and the generation of motor commands using rapid sensory feedback. Although the PMv has frequently been regarded as a single entity, it can be divided into at least five functionally distinct regions: F4, a dorsal convexity region immediately rostral to the primary motor cortex (M1); F5p, a cortical region immediately rostral to F4, lying within the arcuate sulcus; F5c, a ventral convexity region rostral to F4; and F5a, located in the caudal bank of the arcuate sulcus inferior limb lateral to F5p...
April 28, 2018: Neuroscience
https://www.readbyqxmd.com/read/29700566/microeconomic-benefit-of-corneal-transplantation-in-a-developing-country-via-public-private-partnership-model
#9
Rahul M Jindal, Stephen G Waller, Shailendra Sugrim, Jan Pasternak, Joseph Pasternak
BACKGROUND: We measured the microeconomic benefit, QOL, DALYs averted and clinical outcomes of corneal transplant surgery via a public-private partnership in Guyana. Corneas were obtained, ex gratia, from US eye banks, and the work was done at no cost to the patient or the Governments of USA or Guyana. METHODS: We obtained qualitative data using a "semi-structured interview technique" to question 60 recent recipients of corneal transplants in Guyana. Our questions covered schooling in children, training for job, and type and income of job, both before and after surgery...
April 26, 2018: World Journal of Surgery
https://www.readbyqxmd.com/read/29673273/long-term-effects-of-frontalis-fascial-slings-on-the-elastic-properties-of-the-upper-eyelid
#10
Adriano Baccega, Denny Marcos Garcia, Antonio Augusto Velasco Cruz
PURPOSE: To measure the long-term effects of frontalis slings with fascial tissues on the downward eyelid saccadic movements Methods: Downward lid saccades for 10, 20, 30 40 and 50 degrees of downgaze were measured in a sample of 19 patients with congenital ptosis who underwent frontalis slings with fascia lata (autogeneous and banked) and temporalis fascia. Mean postoperative time was 10.7 years ± 3.8 SD. Seventeen age-matched normal subjects comprised the control group. Lid movements as well as the magnitude of brow motion were quantified in all participants with an opto-eletronic device that automatically corrected any head movement...
April 20, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29656016/riding-the-cell-jamming-boundary-geometry-topology-and-phase-of-human-corneal-endothelium
#11
Nigel H Brookes
It is important to assess the viability of eye-banked corneas prior to transplantation due to inherent senescence and known loss of endothelial cells during surgical manipulation. Corneal endothelial cells have a complex basal and paracellular shape making them challenging to accurately measure, particularly in oedematous ex vivo tissue. This study used calibrated centroidal Voronoi Diagrams to segment cells in images of these human corneas, in order to characterize endothelial geometry, topology, and phase...
April 12, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29620567/reduction-of-donor-cornea-exposure-to-repeat-warming-cycles-and-evaluation-times-after-implementation-of-a-rapid-warming-protocol
#12
Jameson Clover, Amy Ansin, Christopher G Stoeger, Mark A Terry, Khoa D Tran
PURPOSE: To compare tissue evaluation times and specular image quality before and after the implementation of rapid tissue warming at a single eye bank. METHODS: This retrospective study compares the evaluation of 494 donor corneas. All corneas were stored in Optisol-GS. Group 1 contained 247 corneas evaluated immediately before implementation of a tissue incubator and tissue warming protocol. Group 2 contained 247 corneas evaluated immediately after implementation...
April 4, 2018: Cornea
https://www.readbyqxmd.com/read/29595764/use-of-donor-corneas-from-pseudophakic-eyes-for-descemet-membrane-endothelial-keratoplasty
#13
Thabo Lapp, Sonja Heinzelmann, Daniel Böhringer, Philipp Eberwein, Thomas Reinhard, Philip Maier
PURPOSE: The prevalence of pseudophakia increases with age and affects more than 30% of people older than 80 years. Because grafts from older donors seem to be more suitable for Descemet membrane endothelial keratoplasty (DMEK) surgery rather than grafts from younger donors, eye bank technicians and corneal surgeons have to deal with corneas from pseudophakic donors for DMEK graft preparation. However, graft preparation from corneas of pseudophakic donors can be more complicated. METHODS: We retrospectively analyzed our donor registry for the prevalence of pseudophakia...
March 27, 2018: Cornea
https://www.readbyqxmd.com/read/29561351/cumulative-endothelial-cell-loss-in-descemet-membrane-endothelial-keratoplasty-grafts-from-preparation-through-insertion-with-glass-injectors
#14
Kenneth Downes, Khoa D Tran, Christopher G Stoeger, Winston Chamberlain
PURPOSE: Evaluation of cumulative Descemet membrane endothelial keratoplasty endothelial cell loss (ECL) from preparation through injection using 2 different glass injectors. METHODS: Eighteen Descemet membrane endothelial keratoplasty grafts with "S" stamps were prepared by eye bank technicians. Nine grafts were assigned to injection with a modified glass Jones tube injector with a 2.4-mm opening and 9 were assigned to injection with the DORC glass pipette injector (<1...
March 20, 2018: Cornea
https://www.readbyqxmd.com/read/29553420/university-students-knowledge-of-corneal-donation-and-willingness-to-donate-corneas-in-the-occupied-palestinian-territory-a-cross-sectional-study
#15
Liana Al-Labadi, Yazan Gammoh, Reham Shehada, Reem Shahin, Walaa Jbarah, Madleen Amro, Hanan Athamny
BACKGROUND: Access to corneal transplantation is limited worldwide because of poor knowledge. Ethical, religious, and cultural barriers contribute to low rates of corneal donation. In the occupied Palestinian territory, limited information is available on factors affecting corneal donation. The aim of this study was to assess the knowledge and willingness towards corneal donation in Palestinian students. METHODS: This cross-sectional study included university students selected through convenience sampling in Nablus in the summer of 2016...
February 21, 2018: Lancet
https://www.readbyqxmd.com/read/29538600/eye-bank-procedures-donor-selection-criteria
#16
Sidney Júlio de Faria E Sousa, Stella Barretto de Faria E Sousa
Eye banks use sterile procedures to manipulate the eye, antiseptic measures for ocular surface decontamination, and rigorous criteria for donor selection to minimize the possibility of disease transmission due to corneal grafting. Donor selection focuses on analysis of medical records and specific post-mortem serological tests. To guide and standardize procedures, eye bank associations and government agencies provide lists of absolute and relative contraindications for use of the tissue based on donor health history...
January 2018: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/29537912/response-properties-of-saccade-related-neurons-of-the-post-arcuate-premotor-cortex
#17
Eleftherios Neromyliotis, Adonis K Moschovakis
We studied the phasic saccade related discharges of single neurons (S neurons) of the premotor cortex of female rhesus monkeys, mostly in the caudal bank of the arcuate sulcus. As described in previous work from our laboratory (Neromyliotis and Moschovakis, 2017b), some of these cells emitted phasic discharges for coordinated movements of the eyes and hand as well as for movements of either effector executed in isolation (motor equivalence -Meq). Other cells (S), did not emit phasic discharges for hand movements unaccompanied by saccades...
March 14, 2018: Journal of Neurophysiology
https://www.readbyqxmd.com/read/29528002/descemet-membrane-endothelial-keratoplasty-outcomes-in-the-first-year-of-experience
#18
Dilek D Altınörs, Leyla Asena
OBJECTIVES: We aimed to report the clinical outcomes of Descemet membrane endothelial keratoplasty in our first year of experience. MATERIALS AND METHODS: Patients who underwent Descemet membrane endothelial keratoplasty at the Baskent University Faculty of Medicine, Department of Ophthalmology, between 2015 and 2016 were included in the study. Patient demographics, cause of endothelial dysfunction, best-corrected visual acuity, central corneal thickness, graft survival, follow-up duration, and intraoperative and postoperative complications were recorded...
March 2018: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29507278/amniotic-membrane-transplantation-in-reconstructive-and-regenerative-ophthalmology
#19
Tobias Röck, Karl Ulrich Bartz-Schmidt, Johanna Landenberger, Matthias Bramkamp, Daniel Röck
BACKGROUND The goal of this study was to investigate numbers, indications, surgical techniques, and experiences of amniotic membrane transplantation at the University Eye Hospital Tübingen over the last 16 years. MATERIAL AND METHODS Data from all amniotic membrane transplantations from January 2001 to December 2016 were retrospectively analyzed. Data was accessed from the electronic database and the annual reports of the Eye Bank at the University Eye Hospital Tübingen. RESULTS A total of 771 amniotic membrane transplantations were performed between 2001 and 2016 at the University Eye Hospital Tübingen...
March 6, 2018: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/29498968/descemet-membrane-endothelial-keratoplasty-learning-curve-for-graft-preparation-in-an-eye-bank-using-645-donor-corneas
#20
Mohit Parekh, Alessandro Ruzza, Vito Romano, Elisa Favaro, Mattia Baruzzo, Gianni Salvalaio, Andrea Grassetto, Stefano Ferrari, Diego Ponzin
PURPOSE: To investigate the learning curve of Descemet membrane endothelial keratoplasty (DMEK) graft preparation in an eye bank. METHODS: Four operators prepared 645 DMEK grafts using the stripping technique between 2014 and 2017 at the Veneto Eye Bank Foundation, Italy. Endothelial cell loss (ECL) and tissue wastage were recorded retrospectively after DMEK preparation and correlated with the number of tissues prepared each year by each operator. On average, our operators performed 1 donor preparation a week over the course of this study...
June 2018: Cornea
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