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Xiaojing Tian, Qianqian Yu, Wei Wu, Xingmin Li, Ruitong Dai
Escherichia coli O157:H7 is an important food-borne pathogenic microorganism that has been used as a model organism for studying microbial inactivation effects and inactivation mechanism in various sterilization technologies. The objective of this study was to investigate the effects of high voltage short time ohmic- (HVST), low voltage long time ohmic- (LVLT), and water bath- (WB) heating on inactivation and proteome changes of E. coli O157:H7 cells at the same endpoint temperature of 72 °C, and to analyze whether a non-thermal death effect existed in ohmic heating...
June 8, 2018: International Journal of Food Microbiology
Lorena Fontán, Qi Qiao, John M Hatcher, Gabriella Casalena, Ilkay Us, Matt Teater, Matthew Durant, Guangyan Du, Min Xia, Natalia Bilchuk, Spandan Chennamadhavuni, Giuseppe Palladino, Giorgio Inghirami, Ulrike Philippar, Hao Wu, David A Scott, Nathanael S Gray, Ari Melnick
The MALT1 paracaspase plays an essential role in Activated B-cell like Diffuse Large B cell Lymphoma (ABC DLBCL) downstream of B cell and Toll-like receptor pathway genes mutated in these tumors. Although MALT1 is considered to be a compelling therapeutic target, development of tractable and specific MALT1 protease inhibitors has thus far been elusive. Herein, we developed a target engagement assay that provides a quantitative readout for specific MALT1 inhibitory effects in living cells. This enabled a structure-guided medicinal chemistry effort culminating in the discovery of pharmacologically tractable irreversible substrate-mimetic compounds that bind the MALT1 active site...
July 19, 2018: Journal of Clinical Investigation
Narong Maneeton, Benchalak Maneeton, Suwannee Putthisri, Pakapan Woottiluk, Assawin Narkpongphun, Manit Srisurapanont
Background: Various clinical trials suggested that risperidone was beneficial in the treatment of autism spectrum disorder (ASD) in children and adolescents. Objective: The aim of this systematic review was to determine the efficacy, acceptability and tolerability of risperidone in the treatment of children and adolescents with ASD. Data sources: The databases of Scopus, PubMed, CINAHL and Cochrane Controlled Trials Register were searched in February 2017...
2018: Neuropsychiatric Disease and Treatment
Yi-Jin Wu, Chun Wang, Wei Wei
The ATP-binding cassette (ABC) transporter family is a large class of ATP energy-dependent transmembrane proteins, and its primary function is to use the energy produced by ATP hydrolysis to transfer the substrate bound to the plasma membrane. This family is also closely related to multidrug resistance (MDR) in various diseases. Among the ABC transporter proteins, P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP) and breast cancer resistance protein (BCRP) are the main members associated with MDR...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Clare J Liddon, Michael E Kelley, Catalina N Rey, Ashley P Liggett, Aurelia Ribeiro
Following reduction, behavior may recur when organisms are exposed to new or different environments. Such recurrences, called treatment relapse, are characterized by an increase of a previously reduced behavior. Renewal is a type of treatment relapse resulting from changes in stimulus contexts despite the ongoing maintenance of treatment. Renewal types include varying arrangements, such as ABA and ABC renewal, where each letter represents a different context. In the present series of translational analyses, we evaluated both ABA and ABC renewal and assessed whether these two renewal arrangements may be present in a single participant's behavior...
July 17, 2018: Journal of Applied Behavior Analysis
Roma Sinha, Mathu Malar C, Raghwan, Subhadeep Das, Sonali Das, Mohammad Shadab, Rukhsana Chowdhury, Sucheta Tripathy, Nahid Ali
Leishmania donovani possesses a complex heteroxenic life cycle where infective metacyclic promastigotes are pre-adapted to infect their host and cope up with intracellular stress. Exploiting the similarities between cultured and sandfly derived promastigotes, we used early and late passage cultured promastigotes to show specific changes at genome level which compromise pathogen fitness reflected in gene expression and infection studies. The pathogen loses virulence mostly via transcriptional and translational regulations and long-time cultivation makes them struggle to convert to virulent metacyclics...
2018: Frontiers in Microbiology
Rosa Helena Bustos-Cruz, Luis Rafael Martínez, Julio César García, George E Barreto, Fernando Suárez
Multidrug resistance-associated proteins (MRP) 1 and 2 belong to the ABC (ATP-Binding Cassette) transporters. These transport proteins are involved in the removal of various drugs and xenobiotics, as well as in multiple physiological, pathological, and pharmacological processes. There is a strong correlation between different polymorphisms and their clinical implication in resistance to antiepileptic drugs, anticancer, and anti-infective agents. In our study, we evaluated exon regions of MRP1 (ABCC1)/MRP2 (ABCC2) in a Colombian cohort of healthy subjects to determine single nucleotide polymorphisms (SNPs) and to determine the allelic and genomic frequency...
July 17, 2018: Pharmaceutics
Hyunkyu Sang, Jonathan P Hulvey, Robert Green, Hao Xu, Jeongdae Im, Taehyun Chang, Geunhwa Jung
Fungi are known to utilize transcriptional regulation of genes that encode efflux transporters to detoxify xenobiotics; however, to date it is unknown how fungi transcriptionally regulate and coordinate different phases of detoxification system (phase I, modification; phase II, conjugation; and phase III, secretion). Here we present evidence of an evolutionary convergence between the fungal and mammalian lineages, whereby xenobiotic detoxification genes (phase I coding for cytochrome P450 monooxygenases [CYP450s] and phase III coding for ATP-binding cassette [ABC] efflux transporters) are transcriptionally regulated by structurally unrelated proteins...
July 17, 2018: MBio
Lu Qu, Yan Jiang, Chongyun Cheng, Dong Wu, Bing Meng, Zhenrong Chen, Yanping Zhu, Neil Shaw, Songying Ouyang, Zhi-Jie Liu
Gene translation requires the correct selection of start codon AUG in mRNA. ATP-binding cassette subfamily F member 1 (ABCF1) plays a key role in the accuracy of start codon selection. However, the function of human ABCF1 is not clearly understood. Here, we solve the crystal structure of an ATP-bound wild-type human ABCF1 at 2.3-Å resolution. The comparative studies indicate that the structure is in a pre-activation intermediate conformation. This conformation is stabilized by the interaction between ATP and protein...
June 12, 2018: Structure
Miguel Rodríguez-Arrastia, Carolina Moreno-Castro, Carmen Ropero-Padilla
OBJECTIVE: To analyse the media representation of the exodus of Spanish nurses to the United Kingdom through general information newspapers. METHODS: Content analysis, quantitative and qualitative. Documents published in the newspapers ABC, El País, El Mundo and La Razón, about "emigrants," "nurses" and "migrant health workers," were located from January 1, 2007, to December 31, 2016, with Boolean operators. This selected period corresponds to the beginning of the economic crisis and concludes in the year of the Brexit referendum in the United Kingdom...
July 14, 2018: Gaceta Sanitaria
Chetan P Phadke, Jaclyn Schwartz, Hubert Vuagnat, Elena Philippou
No abstract text is available yet for this article.
July 13, 2018: Archives of Physical Medicine and Rehabilitation
Suthida Fairee, Santitham Prom-On, Booncharoen Sirinaovakul
In the Artificial Bee Colony (ABC) algorithm, the employed bee and the onlooker bee phase involve updating the candidate solutions by changing a value in one dimension, dubbed one-dimension update process. For some problems which the number of dimensions is very high, the one-dimension update process can cause the solution quality and convergence speed drop. This paper proposes a new algorithm, using reinforcement learning for solution updating in ABC algorithm, called R-ABC. After updating a solution by an employed bee, the new solution results in positive or negative reinforcement applied to the solution dimensions in the onlooker bee phase...
2018: PloS One
Araceli Pérez González, Rigoberto Arámburo-García
: The bilateral cleft lip (BCL) is the most severe manifestation of orofacial clefts. Multiple techniques have been described to reconstruct BCL to obtain good aesthetic and functional results with minimal complications. OBJECTIVE: Description of surgical technique for BCL reconstruction and present the postoperative results obtained. METHODS: Patients with BCL of the cleft lip and palate at ABC Medical Center from June 2013 to June 2017 operated with Mendoza bilateral cheiloplasty were included...
July 13, 2018: Journal of Craniofacial Surgery
Lin-Tong Yang, Jing-Wen Liu, Yan-Mei Wu, Yi-Ping Qi, Jin-Ling Wang, Ning-Wei Lai, Xin Ye, Li-Song Chen
Aluminum (Al)-toxicity and boron (B)-deficiency are two major factors limiting crop production in tropical and subtropical areas. Elevating B supply can alleviate the Al-induced inhibition of growth in Citrus grandis. Seedlings of C. grandis were irrigated for 18 weeks with nutrient solutions containing two B levels (2.5 and 20 μM H3 BO3 ) and two Al levels (0 and 1.2 mM AlCl3 ·6H2 O). By using 2-dimensional electrophoresis (2-DE) based MALDI-TOF/TOF-MS method, this study successfully identified and quantified sixty-one differentially abundant proteins in Citrus roots in response to B-Al interactions...
July 13, 2018: Ecotoxicology and Environmental Safety
Xiujun Zhang, Huiqin Jiang, Jia-Bao Liu, Zehui Shao
The Cartesian product and join are two classical operations in graphs. Let dL(G)(e) be the degree of a vertex e in line graph L(G) of a graph G. The edge versions of atom-bond connectivity (ABCe) and geometric arithmetic (GAe) indices of G are defined as ∑ef∈E(L(G))dL(G)(e)+dL(G)(f)-2dL(G)(e)×dL(G)(f) and ∑ef∈E(L(G))2dL(G)(e)×dL(G)(f)dL(G)(e)+dL(G)(f), respectively. In this paper, ABCe and GAe indices for certain Cartesian product graphs (such as Pn□Pm, Pn□Cm and Pn□Sm) are obtained. In addition, ABCe and GAe indices of certain join graphs (such as Cm+Pn+Sr, Pm+Pn+Pr, Cm+Cn+Cr and Sm+Sn+Sr) are deduced...
July 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Elise Kaplan, Nicholas P Greene, Allister Crow, Vassilis Koronakis
In Gram-negative bacteria, outer-membrane lipoproteins are essential for maintaining cellular integrity, transporting nutrients, establishing infections, and promoting the formation of biofilms. The LolCDE ABC transporter, LolA chaperone, and LolB outer-membrane receptor form an essential system for transporting newly matured lipoproteins from the outer leaflet of the cytoplasmic membrane to the innermost leaflet of the outer membrane. Here, we present a crystal structure of LolA in complex with the periplasmic domain of LolC...
July 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
Daisuke Miura, Kohei Sugiyama, Atsushi Ito, Ayumi Ohba-Tanaka, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
For strain improvement of Aspergillus oryzae, development of the transformation system is essential, wherein dominant selectable markers, including drug-resistant genes, are available. However, A. oryzae generally has a relatively high resistance to many antifungal drugs effective against yeasts and other filamentous fungi. In the course of the study, while investigating azole drug resistance in A. oryzae, we isolated a spontaneous mutant that exhibited high resistance to azole fungicides and found that pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter genes were upregulated in the mutant; their overexpression in the wild-type strain increased azole drug resistance...
July 16, 2018: Bioscience, Biotechnology, and Biochemistry
Hirotatsu Imai, Takaya Abe, Tomohiro Miyoshi, Shuh-Ichi Nishikawa, Kosuke Ito, Toshio Uchiumi
The ATP-binding cassette (ABC) protein ABCE1 is an essential factor in ribosome recycling during translation. However, the detailed mechanochemistry of its recruitment to the ribosome, ATPase activation and subunit dissociation remain to be elucidated. Here, we show that the ribosomal stalk protein, which is known to participate in the actions of translational GTPase factors, plays an important role in these events. Biochemical and crystal structural data indicate that the conserved hydrophobic amino acid residues at the C-terminus of the archaeal stalk protein aP1 binds to the nucleotide-binding domain 1 (NBD1) of aABCE1, and that this binding is crucial for ATPase activation of aABCE1 on the ribosome...
July 13, 2018: Nucleic Acids Research
Olivia Eriksson, Alexandra Jauhiainen, Sara Maad Sasane, Andrei Kramer, Anu G Nair, Carolina Sartorius, Jeanette Hellgren Kotaleski
Motivation: Dynamical models describing intracellular phenomena are increasing in size and complexity as more information is obtained from experiments. These models are often over-parameterized with respect to the quantitative data used for parameter estimation, resulting in uncertainty in the individual parameter estimates as well as in the predictions made from the model. Here we combine Bayesian analysis with global sensitivity analysis in order to give better informed predictions; to point out weaker parts of the model that are important targets for further experiments, as well as to give guidance on parameters that are essential in distinguishing different qualitative output behaviours...
July 13, 2018: Bioinformatics
Sofia H Piltz, Lauri Harhanen, Mason A Porter, Philip K Maini
We construct two ordinary-differential-equation models of a predator feeding adaptively on two prey types, and we evaluate the models' ability to fit data on freshwater plankton. We model the predator's switch from one prey to the other in two different ways: (i) smooth switching using a hyperbolic tangent function; and (ii) by incorporating a parameter that changes abruptly across the switching boundary as a system variable that is coupled to the population dynamics. We conduct linear stability analyses, use approximate Bayesian computation (ABC) combined with a population Monte Carlo (PMC) method to fit model parameters, and compare model results quantitatively to data for ciliate predators and their two algal prey groups collected from Lake Constance on the German-Swiss-Austrian border...
July 13, 2018: Journal of Theoretical Biology
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