keyword
MENU ▼
Read by QxMD icon Read
search

Dictyostelium

keyword
https://www.readbyqxmd.com/read/28727774/inhibitors-of-mycobacterium-marinum-virulence-identified-in-a-dictyostelium-discoideum-host-model
#1
Hajer Ouertatani-Sakouhi, Sébastien Kicka, Gianpaolo Chiriano, Christopher F Harrison, Hubert Hilbi, Leonardo Scapozza, Thierry Soldati, Pierre Cosson
Tuberculosis remains one of the major threats to public health worldwide. Given the prevalence of multi drug resistance (MDR) in Mycobacterium tuberculosis strains, there is a strong need to develop new anti-mycobacterial drugs with modes of action distinct from classical antibiotics. Inhibitors of mycobacterial virulence might target new molecular processes and may represent a potential new therapeutic alternative. In this study, we used a Dictyostelium discoideum host model to assess virulence of Mycobacterium marinum and to identify compounds inhibiting mycobacterial virulence...
2017: PloS One
https://www.readbyqxmd.com/read/28719054/evolutionary-cell-biology-of-proteins-from-protists-to-humans-and-plants
#2
REVIEW
Helmut Plattner
During evolution, the cell as a fine-tuned machine had to undergo permanent adjustments to match changes in its environment, while "closed for repair work" was not possible. Evolution from protists (protozoa and unicellular algae) to multicellular organisms may have occurred in basically two lineages, Unikonta and Bikonta, culminating in mammals and angiosperms (flowering plants), respectively. Unicellular models for unikont evolution are myxamoebae (Dictyostelium) and increasingly also choanoflagellates, whereas, for bikonts, ciliates are preferred models...
July 18, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28714585/mathematical-model-of-collective-cell-migrations-based-on-cell-polarity
#3
Masakazu Akiyama, Takamichi Sushida, Sumire Ishida, Hisashi Haga
Individual cells migrate toward the direction of the cell polarity generated by interior or exterior factors. Under situations without guides such as chemoattractants, they migrate randomly. On the other hand, it has been observed that cell groups lead to systematic collective cell migrations. For example, Dictyostelium discoideum and Madin-Darby canine kidney (epithelial) cells exhibit typical collective cell migration patterns such as uniformly directional migration and rotational migration. In particular, it has been suggested from experimental investigations that rotational migrations are intimately related to morphogenesis of organs and tissues in several species...
July 17, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28711497/theaflavins-from-black-tea-affect-growth-development-and-motility-in-dictyostelium-discoideum
#4
April N Ilacqua, Joshua A Shettler, Kevin M Wernke, Jessah K Skalla, Kyle J McQuade
Theaflavins, flavonoids found in black tea, exhibit a variety of health-promoting activities, but the mechanisms by which they act are not clear. Here, we assess the effects of black tea extract and isolated theaflavins on Dictyostelium discoideum, a model organism exhibiting an unusual life cycle relying on conserved pathways involved in human disease. Dictyostelium has been used to characterize the activities of numerous bioactive small molecules, including catechins, from which theaflavins are produced during the preparation of black tea...
July 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28701378/dictyostelium-ampk%C3%AE-regulates-aggregate-size-and-cell-type-patterning
#5
Ranjana Maurya, Rakesh Kumar, Shweta Saran
Starved Dictyostelium cells aggregate into groups of nearly 10(5) cells. AMPK is a highly conserved serine/threonine protein kinase consisting of a catalytic and two regulatory subunits. As multi-cellular development in Dictyostelium is initiated upon starvation, we explored the role of the energy sensor, AMPK, which shows significant similarity to human AMPK and is expressed throughout development. Deletion of the ampkα gene results in the formation of numerous small-sized aggregates that develop asynchronously to form few fruiting bodies with small sori and long stalks...
July 2017: Open Biology
https://www.readbyqxmd.com/read/28696553/mechanisms-of-the-diterpene-cyclases-%C3%AE-pinacene-synthase-from-dictyostelium-discoideum-and-hydropyrene-synthase-from-streptomyces-clavuligerus
#6
Jan Rinkel, Patrick Rabe, Xinlu Chen, Tobias G Köllner, Feng Chen, Jeroen S Dickschat
Two diterpene cyclases, one from the social amoeba Dictyostelium discoideum and the other from the bacterium Streptomyces clavuligerus, with products containing a Z-configured double bond between the original C2 and C3 of geranylgeranyl diphosphate, were extensively investigated for their mechanisms through isotopic labelling experiments. The participation of geranyllinalyl diphosphate, in analogy to the role of linalyl and nerolidyl diphosphate for mono- and sesquiterpene biosynthesis, as an intermediate towards diterpenes with a Z-configured C2=C3 double bond is discussed...
July 11, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28680861/nramp1-and-nrampb-contribute-to-resistance-against-francisella-in-dictyostelium
#7
Yannick Brenz, Denise Ohnezeit, Hanne C Winther-Larsen, Monica Hagedorn
The Francisella genus comprises highly pathogenic bacteria that can cause fatal disease in their vertebrate and invertebrate hosts including humans. In general, Francisella growth depends on iron availability, hence, iron homeostasis must be tightly regulated during Francisella infection. We used the system of the professional phagocyte Dictyostelium and the fish pathogen F. noatunensis subsp. noatunensis (F.n.n.) to investigate the role of the host cell iron transporters Nramp (natural resistance associated macrophage proteins) during Francisella infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28671610/methods-to-monitor-and-quantify-autophagy-in-the-social-amoeba-dictyostelium-discoideum
#8
REVIEW
Eunice Domínguez-Martín, Elena Cardenal-Muñoz, Jason S King, Thierry Soldati, Roberto Coria, Ricardo Escalante
Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial...
July 3, 2017: Cells
https://www.readbyqxmd.com/read/28665127/activity-of-discadenine-as-a-plant-cytokinin
#9
Václav Mik, Zuzana Mičková, Karel Doležal, Ivo Frébort, Tomáš Pospíšil
Discadenine (1), a self-spore germination inhibitor from the cellular slim mold Dictyostelium discoideum, is structurally related to the plant hormone cytokinin. This compound was synthesized from l-aspartic acid, and its activities were confirmed by three classical cytokinin bioassays as well as by using binding and activation assays with the Arabidopsis cytokinin receptors AHK3 and CRE1/AHK4.
June 30, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28663788/using-spectral-decomposition-of-the-signals-from-laurdan-derived-probes-to-evaluate-the-physical-state-of-membranes-in-live-cells
#10
Serge Mazeres, Farzad Fereidouni, Etienne Joly
Background: We wanted to investigate the physical state of biological membranes in live cells under the most physiological conditions possible. Methods: For this we have been using laurdan, C-laurdan or M-laurdan to label a variety of cells, and a biphoton microscope equipped with both a thermostatic chamber and a spectral analyser. We also used a flow cytometer to quantify the 450/530 nm ratio of fluorescence emissions by whole cells. Results: We find that using all the information provided by spectral analysis to perform spectral decomposition dramatically improves the imaging resolution compared to using just two channels, as commonly used to calculate generalized polarisation (GP)...
2017: F1000Research
https://www.readbyqxmd.com/read/28641070/elmo-proteins-transduce-g-protein-coupled-receptor-signal-to-control-reorganization-of-actin-cytoskeleton-in-chemotaxis-of-eukaryotic-cells
#11
Xuehua Xu, Tian Jin
Chemotaxis, which is chemoattractant-guided directional cell migration, plays major roles in recruitment of neutrophils, the metastasis of cancer cells, and the development of the model organism Dictyostelium discoideum. These cells share remarkable similarities in the signaling pathways by which they control chemotaxis. They all use a G protein-coupled receptor (GPCR)-mediated signal transduction pathway to sense the chemotactic gradient to guide cell migration. Diverse chemokines activate Rac through conserved GPCR signaling pathways...
June 22, 2017: Small GTPases
https://www.readbyqxmd.com/read/28636923/mechanosensitive-adhesion-explains-stepping-motility-in-amoeboid-cells
#12
Calina A Copos, Sam Walcott, Juan C Del Álamo, Effie Bastounis, Alex Mogilner, Robert D Guy
Cells employing amoeboid motility exhibit repetitive cycles of rapid expansion and contraction and apply coordinated traction forces to their environment. Although aspects of this process are well studied, it is unclear how the cell controls the coordination of cell length changes with adhesion to the surface. Here, we develop a simple model to mechanistically explain the emergence of periodic changes in length and spatiotemporal dynamics of traction forces measured in chemotaxing unicellular amoeba, Dictyostelium discoideum...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28633918/traction-force-and-its-regulation-during-cytokinesis-in-dictyostelium-cells
#13
Md Golam Sarowar Jahan, Shigehiko Yumura
Cytokinesis is the final stage of cell division. Dictyostelium cells have multiple modes of cytokinesis, including cytokinesis A, B and C. Cytokinesis A is a conventional mode, which depends on myosin II in the contractile ring. Myosin II null cells divide depending on substratum-attachment (cytokinesis B) or in a multi-polar fashion independent of the cell cycle (cytokinesis C). We investigated the traction stress exerted by dividing cells in the three different modes using traction force microscopy. In all cases, the traction forces were directed inward from both poles...
June 12, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28632994/role-of-the-small-gtpase-rap1-in-signal-transduction-cell-dynamics-and-bacterial-infection
#14
Hubert Hilbi, Arjan Kortholt
Rap1 belongs to the Ras family of small GTPases, which are involved in a multitude of cellular signal transduction pathways and have extensively been linked to cancer biogenesis and metastasis. The small GTPase is activated in response to various extracellular and intracellular cues. Rap1 has conserved functions in Dictyostelium discoideum amoeba and mammalian cells, which are important for cell polarity, substrate and cell-cell adhesion and other processes that involve the regulation of cytoskeletal dynamics...
June 20, 2017: Small GTPases
https://www.readbyqxmd.com/read/28630813/mutations-in-the-sh1-helix-alter-the-thermal-properties-of-myosin-ii
#15
Kotomi Shibata, Tsubasa Koyama, Shohei Inde, Sosuke Iwai, Shigeru Chaen
The myosin II SH1 helix is a joint that links the converter subdomain to the rest of the myosin motor domain and possibly plays a key role in the arrangement of the converter/lever arm. Several point mutations within the SH1 helix in human myosin IIs have been shown to cause diseases. To reveal whether these SH1 helix mutations affect not only motile activities but also thermal properties of myosin II, here we introduced the E683K or R686C point mutation into the SH1 helix in Dictyostelium myosin II. Thermal inactivation as well as thermal aggregation rates of these mutant proteins demonstrated that these mutations decreased the thermal stability of myosin II...
2017: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/28619991/evidence-that-differentiation-inducing-factor-1-controls-chemotaxis-and-cell-differentiation-at-least-in-part-via-mitochondria-in-d-discoideum
#16
Yuzuru Kubohara, Haruhisa Kikuchi, Van Hai Nguyen, Hidekazu Kuwayama, Yoshiteru Oshima
Differentiation-inducing factor-1 [1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one (DIF-1)] is an important regulator of cell differentiation and chemotaxis in the development of the cellular slime mold Dictyostelium discoideum However, the entire signaling pathways downstream of DIF-1 remain to be elucidated. To characterize DIF-1 and its potential receptor(s), we synthesized two fluorescent derivatives of DIF-1, boron-dipyrromethene (BODIPY)-conjugated DIF-1 (DIF-1-BODIPY) and nitrobenzoxadiazole (NBD)-conjugated DIF-1 (DIF-1-NBD), and investigated their biological activities and cellular localization...
June 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28602650/generation-of-single-cell-transcript-variability-by-repression
#17
Vlatka Antolović, Agnes Miermont, Adam M Corrigan, Jonathan R Chubb
Gene expression levels vary greatly within similar cells, even within clonal cell populations [1]. These spontaneous expression differences underlie cell fate diversity in both differentiation and disease [2]. The mechanisms responsible for generating expression variability are poorly understood. Using single-cell transcriptomics, we show that transcript variability emerging during Dictyostelium differentiation is driven predominantly by repression rather than activation. The increased variability of repressed genes was observed over a broad range of expression levels, indicating that variability is actively imposed and not a passive statistical effect of the reduced numbers of molecules accompanying repression...
June 19, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28584190/extracellular-polyphosphate-signals-through-ras-and-akt-to-prime-dictyostelium-discoideum-cells-for-development
#18
Patrick M Suess, Jacob Watson, Wensheng Chen, Richard H Gomer
Linear chains of five to hundreds of phosphates called polyphosphate are found in organisms ranging from bacteria to humans, but their function is poorly understood. In Dictyostelium discoideum, polyphosphate is used as a secreted signal that inhibits cytokinesis in an autocrine negative feedback loop. To elucidate how cells respond to this unusual signal, we undertook a proteomic analysis of cells treated with physiological levels of polyphosphate and observed that polyphosphate causes cells to decrease levels of actin cytoskeleton proteins, possibly explaining how polyphosphate inhibits cytokinesis...
July 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28578698/dictyexpress-a-web-based-platform-for-sequence-data-management-and-analytics-in-dictyostelium-and-beyond
#19
Miha Stajdohar, Rafael D Rosengarten, Janez Kokosar, Luka Jeran, Domen Blenkus, Gad Shaulsky, Blaz Zupan
BACKGROUND: Dictyostelium discoideum, a soil-dwelling social amoeba, is a model for the study of numerous biological processes. Research in the field has benefited mightily from the adoption of next-generation sequencing for genomics and transcriptomics. Dictyostelium biologists now face the widespread challenges of analyzing and exploring high dimensional data sets to generate hypotheses and discovering novel insights. RESULTS: We present dictyExpress (2.0), a web application designed for exploratory analysis of gene expression data, as well as data from related experiments such as Chromatin Immunoprecipitation sequencing (ChIP-Seq)...
June 2, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28556908/hydrophilic-interaction-anion-exchange-for-separation-of-multiply-modified-neutral-and-anionic-dictyostelium-n-glycans
#20
Alba Hykollari, Daniel Malzl, Shi Yan, Iain B H Wilson, Katharina Paschinger
The unusual nature of the N-glycans of the cellular slime mould Dictyostelium discoideum has been revealed by a number of studies, primarily based on examination of radiolabeled glycopeptides but more recently also by MS. The complexity of the N-glycomes of even glycosylation mutants is compounded by the occurrence of anionic modifications, which also present an analytical challenge. In this study, we have employed hydrophilic interaction anion exchange (HIAX) HPLC in combination with MALDI-TOF MS/MS to explore the anionic N-glycome of the M31 (modA) strain, which lacks endoplasmic reticulum α-glucosidase II, an enzyme conserved in most eukaryotes including Homo sapiens...
May 29, 2017: Electrophoresis
keyword
keyword
39985
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"