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Dictyostelium

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https://www.readbyqxmd.com/read/28211207/social-amoebae-mating-types-do-not-invest-unequally-in-sexual-offspring
#1
Tracy E Douglas, David C Queller, Joan E Strassmann
Unequal investment by different sexes in their progeny is common and includes differential investment in the zygote and differential care of the young. The social amoeba Dictyostelium discoideum has a sexual stage in which isogamous cells of any two of the three mating types fuse to form a zygote which then attracts hundreds of other cells to the macrocyst. The latter cells are cannibalized and so make no genetic contribution to reproduction. Previous literature suggests that this sacrifice may be induced in cells of one mating type by cells of another, resulting in a higher than expected production of macrocysts when the inducing type is rare and a giving a reproductive advantage to this social cheat...
February 17, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/28210928/bacterial-resistance-to-arsenic-protects-against-protist-killing
#2
Xiuli Hao, Xuanji Li, Chandan Pal, Jon Hobman, D G Joakim Larsson, Quaiser Saquib, Hend A Alwathnani, Barry P Rosen, Yong-Guan Zhu, Christopher Rensing
Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyostelium discoideum (D. discoideum). Deletion of the E. coli ars operon led to significantly lower intracellular survival compared to wild type E. coli. This suggests that protists use arsenic to poison bacterial cells in the phagosome, similar to their use of copper...
February 16, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28205535/phylogenetic-analysis-of-eukaryotic-neet-proteins-uncovers-a-link-between-a-key-gene-duplication-event-and-the-evolution-of-vertebrates
#3
Madhuri A Inupakutika, Soham Sengupta, Rachel Nechushtai, Patricia A Jennings, Jose' N Onuchic, Rajeev K Azad, Pamela Padilla, Ron Mittler
NEET proteins belong to a unique family of iron-sulfur proteins in which the 2Fe-2S cluster is coordinated by a CDGSH domain that is followed by the "NEET" motif. They are involved in the regulation of iron and reactive oxygen metabolism, and have been associated with the progression of diabetes, cancer, aging and neurodegenerative diseases. Despite their important biological functions, the evolution and diversification of eukaryotic NEET proteins are largely unknown. Here we used the three members of the human NEET protein family (CISD1, mitoNEET; CISD2, NAF-1 or Miner 1; and CISD3, Miner2) as our guides to conduct a phylogenetic analysis of eukaryotic NEET proteins and their evolution...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28186323/the-arp2-3-inhibitory-protein-arpin-is-dispensable-for-chemotaxis
#4
Irène Dang, Joern Linkner, Jun Yan, Daniel Irimia, Jan Faix, Alexis Gautreau
Arpin is an Arp2/3 inhibitory protein, which decreases the protrusion lifetime and hence directional persistence in the migration of diverse cells. Arpin is activated by the small GTPase Rac, which controls cell protrusion, thus closing a negative feedback loop that renders the protrusion intrinsically unstable. Because of these properties, it was proposed that Arpin might play a role in directed migration, where directional persistence has to be fine-tuned. We report here, however, that Arpin depleted tumor cells and Arpin knock-out Dictyostelium amoeba display no obvious defect in chemotaxis...
February 10, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28183814/comparative-proteomics-of-purified-pathogen-vacuoles-correlates-intracellular-replication-of-legionella-pneumophila-with-the-small-gtpase-rap1
#5
Johanna Schm Oumllders, Christian Manske, Andreas Otto, Christine Hoffmann, Bernhard Steiner, Amanda Welin, D Oumlrte Becher, Hubert Hilbi
Legionella pneumophila is an opportunistic bacterial pathogen that causes a severe lung infection termed Legionnaires disease. The pathogen replicates in environmental protozoa as well as in macrophages within a unique membrane-bound compartment, the Legionella-containing-vacuole (LCV). LCV formation requires the bacterial Icm/Dot type IV secretion system, which translocates ca. 300 effector proteins into host cells, where they target distinct host factors. The L. pneumophila pentuple mutant (Δpentuple) lacks 5 gene clusters (31% of the effector proteins) and replicates in macrophages but not in Dictyostelium discoideum amoeba...
February 9, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28158557/effects-of-deletion-of-the-receptor-crla-on-dictyostelium-aggregation-and-mpbd-mediated-responses-are-strain-dependent-and-not-evident-in-strain-ax2
#6
Takaaki B Narita, Pauline Schaap, Tamao Saito
No abstract text is available yet for this article.
February 2, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28136018/co-existence-of-ras-activation-in-a-chemotactic-signal-transduction-pathway-and-in-an-autonomous-wave-forming-system
#7
Mary Ecke, Günther Gerisch
The activation of Ras is common to two activities in cells of Dictyostelium discoideum: the directed movement in a gradient of chemoattractant and the autonomous generation of propagating waves of actin polymerization on the substrate-attached cell surface. We produced large cells by electric-pulse induced fusion to simultaneously study both activities in one cell. For imaging, a fluorescent label for activated Ras was combined with labels for filamentous actin, PIP3, or PTEN. Chemotactic responses were elicited in a diffusion gradient of cyclic AMP...
January 31, 2017: Small GTPases
https://www.readbyqxmd.com/read/28128835/identification-of-novel-inhibitors-of-the-translationally-controlled-tumor-protein-tctp-insights-from-molecular-dynamics
#8
Rakesh Kumar, Ranjana Maurya, Shweta Saran
The translationally controlled tumor protein (TCTP) is a highly conserved multifunctional protein, preferentially expressed in mitotically active tissues and is a potential biomarker and a therapeutic target for lung cancers. An understanding of the biology of this molecule and model systems for the screening of drugs is still awaited. In the absence of complete crystal structure, NMR structures as templates were used for homology modeling and MD optimization of both Dictyostelium discoideum and human TCTPs, which was followed by pocket-site prediction, ligand screening and docking...
January 27, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28124777/minimal-amino-acids-in-the-i-lweq-domain-required-for-anterior-posterior-localization-in-dictyostelium
#9
Hyeseon Kim, Dong-Yeop Shin, Taeck Joong Jeon
Establishment of cell polarity is mediated by a series of signaling molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localization could, with some additional deletion at the C-terminal, localize to the anterior...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28122819/method-to-study-cell-migration-under-uniaxial-compression
#10
Nishit Srivastava, Robert R Kay, Alexandre J Kabla
The chemical, physical and mechanical properties of the extra-cellular environment have a strong effect on cell migration. Aspects such as pore-size or stiffness of the matrix influence the selection of the mechanism used by cells to propel themselves, including pseudopod or blebbing. How a cell perceives its environment, and how such a cue triggers a change in behaviour are largely unknown, but mechanics is likely to be involved. Because mechanical conditions are often controlled by modifying the composition of the environment, separating chemical and physical contributions is difficult and requires multiple controls...
January 25, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28117457/the-aliphatic-amidase-amie-is-involved-in-regulation-of-pseudomonas-aeruginosa-virulence
#11
Thomas Clamens, Thibaut Rosay, Alexandre Crépin, Teddy Grandjean, Takfarinas Kentache, Julie Hardouin, Perrine Bortolotti, Anke Neidig, Marlies Mooij, Mélanie Hillion, Julien Vieillard, Pascal Cosette, Joerg Overhage, Fergal O'Gara, Emeline Bouffartigues, Alain Dufour, Sylvie Chevalier, Benoit Guery, Pierre Cornelis, Marc G J Feuilloley, Olivier Lesouhaitier
We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28104305/cp39-cp75-and-cp91-are-major-structural-components-of-the-dictyostelium-centrosome-s-core-structure
#12
Irene Meyer, Tatjana Peter, Petros Batsios, Oliver Kuhnert, Anne Krüger-Genge, Carl Camurça, Ralph Gräf
The acentriolar Dictyostelium centrosome is a nucleus-associated body consisting of a core structure with three plaque-like layers, which are surrounded by a microtubule-nucleating corona. The core duplicates once per cell cycle at the G2/M transition, whereby its central layer disappears and the two outer layers form the mitotic spindle poles. Through proteomic analysis of isolated centrosomes, we have identified CP39 and CP75, two essential components of the core structure. Both proteins can be assigned to the central core layer as their centrosomal presence is correlated to the disappearance and reappearance of the central core layer in the course of centrosome duplication...
January 12, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28103313/mycobacterium-marinum-degrades-both-triacylglycerols-and-phospholipids-from-its-dictyostelium-host-to-synthesise-its-own-triacylglycerols-and-generate-lipid-inclusions
#13
Caroline Barisch, Thierry Soldati
During a tuberculosis infection and inside lipid-laden foamy macrophages, fatty acids (FAs) and sterols are the major energy and carbon source for Mycobacterium tuberculosis. Mycobacteria can be found both inside a vacuole and the cytosol, but how this impacts their access to lipids is not well appreciated. Lipid droplets (LDs) store FAs in form of triacylglycerols (TAGs) and are energy reservoirs of prokaryotes and eukaryotes. Using the Dictyostelium discoideum/Mycobacterium marinum infection model we showed that M...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28076662/vps13f-links-bacterial-recognition-and-intracellular-killing-in-dictyostelium
#14
Jade Leiba, Ayman Sabra, Romain Bodinier, Anna Marchetti, Wanessa C Lima, Astrid Melotti, Jackie Perrin, Frederic Burdet, Marco Pagni, Thierry Soldati, Emmanuelle Lelong, Pierre Cosson
Bacterial sensing, ingestion and killing by phagocytic cells are essential processes to protect the human body from infectious microorganisms. The cellular mechanisms involved in intracellular killing, their relative importance, and their specificity towards different bacteria are however poorly defined. In this study, we used Dictyostelium discoideum, a phagocytic cell model amenable to genetic analysis, to identify new gene products involved in intracellular killing. A random genetic screen led us to identify the role of Vps13F in intracellular killing of Klebsiella pneumoniae...
January 11, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28074884/actin-interacting-protein-1-contributes-to-intranuclear-rod-assembly-in-dictyostelium-discoideum
#15
Hellen C Ishikawa-Ankerhold, Wioleta Daszkiewicz, Michael Schleicher, Annette Müller-Taubenberger
Intranuclear rods are aggregates consisting of actin and cofilin that are formed in the nucleus in consequence of chemical or mechanical stress conditions. The formation of rods is implicated in a variety of pathological conditions, such as certain myopathies and some neurological disorders. It is still not well understood what exactly triggers the formation of intranuclear rods, whether other proteins are involved, and what the underlying mechanisms of rod assembly or disassembly are. In this study, Dictyostelium discoideum was used to examine appearance, stages of assembly, composition, stability, and dismantling of rods...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28060267/studying-the-protein-quality-control-system-of-d-discoideum-using-temperature-controlled-live-cell-imaging
#16
Liliana Malinovska, Simon Alberti
The complex lifestyle of the social amoebae Dictyostelium discoideum makes it a valuable model for the study of various biological processes. Recently, we showed that D. discoideum is remarkably resilient to protein aggregation and can be used to gain insights into the cellular protein quality control system. However, the use of D. discoideum as a model system poses several challenges to microscopy-based experimental approaches, such as the high motility of the cells and their susceptibility to photo-toxicity...
December 2, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28057864/adenylate-cyclase-a-acting-on-pka-mediates-induction-of-stalk-formation-by-cyclic-diguanylate-at-the-dictyostelium-organizer
#17
Zhi-Hui Chen, Reema Singh, Christian Cole, Hajara Mohammed Lawal, Christina Schilde, Melanie Febrer, Geoffrey J Barton, Pauline Schaap
Coordination of cell movement with cell differentiation is a major feat of embryonic development. The Dictyostelium stalk always forms at the organizing tip, by a mechanism that is not understood. We previously reported that cyclic diguanylate (c-di-GMP), synthesized by diguanylate cyclase A (DgcA), induces stalk formation. Here we used transcriptional profiling of dgca- structures to identify target genes for c-di-GMP, and used these genes to investigate the c-di-GMP signal transduction pathway. We found that knockdown of cAMP-dependent protein kinase (PKA) activity in prestalk cells reduced stalk gene induction by c-di-GMP, whereas PKA activation bypassed the c-di-GMP requirement for stalk gene expression...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28055008/early-nucleolar-disorganization-in-dictyostelium-cell-death
#18
M F Luciani, Y Song, A Sahrane, A Kosta, P Golstein
Cell death occurs in all eukaryotes, but it is still not known whether some core steps of the cell death process are conserved. We investigated this using the protist Dictyostelium. The dissection of events in Dictyostelium vacuolar developmental cell death was facilitated by the sequential requirement for two distinct exogenous signals. An initial exogenous signal (starvation and cAMP) recruited some cells into clumps. Only within these clumps did subsequent cell death events take place. Contrary to our expectations, already this initial signal provoked nucleolar disorganization and irreversible inhibition of rRNA and DNA synthesis, reflecting marked cell dysfunction...
January 5, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28049717/a-novel-hect-ubiquitin-ligase-regulating-chemotaxis-and-development-in-dictyostelium-discoideum
#19
Barbara Pergolizzi, Enrico Bracco, Salvatore Bozzaro
Cyclic AMP binding to G protein-coupled receptors orchestrates chemotaxis and development in Dictyostelium. By activating the RasC-TORC2-AKT/PKB module, cAMP regulates cell polarization during chemotaxis. TORC2 also mediates GPCR-dependent stimulation of adenylyl cyclase A (ACA), enhancing cAMP relay and developmental gene expression. Thus, mutants defective in the TORC2 Pia/Rictor subunit are impaired in chemotaxis and development. Near-saturation mutagenesis of a Pia/Rictor mutant by random gene disruption led to selection of two suppressor mutants, in which spontaneous chemotaxis and development were restored...
January 3, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28028841/functional-similarities-between-the-dictyostelium-protein-apra-and-the-human-protein-dipeptidyl-peptidase-iv
#20
Sarah E Herlihy, Yu Tang, Jonathan E Phillips, Richard H Gomer
Autocrine proliferation repressor protein A (AprA) is a protein secreted by Dictyostelium discoideum cells. Although there is very little sequence similarity between AprA and any human protein, AprA has a predicted structural similarity to the human protein Dipeptidyl Peptidase IV (DPPIV). AprA is a chemorepellent for Dictyostelium cells, and DPPIV is a chemorepellent for neutrophils. This led us to investigate if AprA and DPPIV have additional functional similarities. We find that like AprA, DPPIV is a chemorepellent for, and inhibits the proliferation of, D...
December 28, 2016: Protein Science: a Publication of the Protein Society
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