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https://www.readbyqxmd.com/read/28330902/regulation-of-nucleosome-positioning-by-a-chd-type-iii-chromatin-remodeler-and-its-relationship-to-developmental-gene-expression-in-dictyostelium
#1
James L Platt, Nicholas A Kent, Alan R Kimmel, Adrian J Harwood
Nucleosome placement and repositioning can direct transcription of individual genes; however, the precise interactions of these events are complex and largely unresolved at the whole-genome level. The Chromodomain-Helicase-DNA binding (CHD) Type III proteins are a subfamily of SWI2/SNF2 proteins that control nucleosome positioning and are associated with several complex human disorders, including CHARGE syndrome and autism. Type III CHDs are required for multicellular development of animals and Dictyostelium but are absent in plants and yeast...
March 22, 2017: Genome Research
https://www.readbyqxmd.com/read/28330789/schiff-bases-of-putrescine-with-methylglyoxal-protect-from-cellular-damage-caused-by-accumulation-of-methylglyoxal-and-reactive-oxygen-species-in-dictyostelium-discoideum
#2
Seong-Jun Park, Min-Kyu Kwak, Sa-Ouk Kang
Polyamines protect protein glycation in cells against the advanced glycation end product precursor methylglyoxal, which is inevitably produced during glycolysis, and the enzymes that detoxify this α-ketoaldehyde have been widely studied. Nonetheless, nonenzymatic methylglyoxal-scavenging molecules have not been sufficiently studied either in vitro or in vivo. Here, we hypothesized reciprocal regulation between polyamines and methylglyoxal modeled in Dictyostelium grown in a high-glucose medium. We based our hypothesis on the reaction between putrescine and methylglyoxal in putrescine-deficient (odc(-)) or putrescine-overexpressing (odc(oe)) cells...
March 18, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28302905/protein-kinase-a-regulates-the-ras-rap1-and-torc2-pathways-in-response-to-the-chemoattractant-camp-in-dictyostelium
#3
Margarethakay Scavello, Alexandra R Petlick, Ramya Ramesh, Valery F Thompson, Pouya Lotfi, Pascale G Charest
Efficient directed migration requires tight regulation of chemoattractant signal transduction pathways in both space and time, but the mechanisms involved in such regulation are not well understood. Here, we investigated the role of Protein Kinase A (PKA) in controlling signaling of the chemoattractant cAMP in Dictyostelium We found that cells lacking PKA display severe chemotaxis defects, including impaired directional sensing. Although PKA is an important regulator of developmental gene expression, including the cAMP receptor cAR1, our studies using exogenously expressed cAR1 in cells lacking PKA, cells lacking adenylyl cyclase A (ACA), and cells treated with the PKA-selective pharmacological inhibitor H89, suggest that PKA controls chemoattractant signal transduction, in part, through the regulation of RasG, Rap1, and TORC2...
March 16, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28287245/yela-a-putative-dictyostelium-translational-regulator-acts-as-antagonist-of-dif-1-signaling-to-control-cell-type-proportioning
#4
Yoko Yamada, Chris Sugden, Jeffrey G Williams
DIF-1 (differentiation-inducing factor1) is a polyketide produced by Dictyostelium prespore cells which induces initially uncommitted cells to differentiate as prestalk cells. Exposure of cells to DIF-1 causes transitory hypo-phosphorylation of seven serine residues in YelA, a protein with a region of strong homology to the MIF4G domain of the eukaryotic initiation factor eIF4G. Based upon its domain architecture, which in one important aspect closely resembles that of Death-Associated Protein 5 (DAP5), we predict a role in stimulating internal ribosome entry-driven mRNA translation...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28272354/micrometer-scale-membrane-transition-of-supported-lipid-bilayer-membrane-reconstituted-with-cytosol-of-dictyostelium-discoideum
#5
Kei Takahashi, Taro Toyota
The transformation of the supported lipid bilayer (SLB) membrane by extracted cytosol from living resources, has recently drawn much attention. It enables us to address the question of whether the purified phospholipid SLB membrane, including lipids related to amoeba locomotion, which was discussed in many previous studies, exhibits membrane deformation in the presence of cytosol extracted from amoeba; Methods: In this report, a method for reconstituting a supported lipid bilayer (SLB) membrane, composed of purified phospholipids and cytosol extracted from Dictyostelium discoideum, is described...
March 7, 2017: Life
https://www.readbyqxmd.com/read/28264855/structural-alteration-of-ompr-as-a-source-of-ertapenem-resistance-in-a-ctx-m-15-producing-escherichia-coli-o25b-h4-st131-clinical-isolate
#6
Hervé Dupont, Pascaline Choinier, David Roche, Sandine Adiba, Megan Sookdeb, Catherine Branger, Erick Denamur, Hedi Mammeri
In this study, an ertapenem-non-susceptible Escherichia coli isolate was investigated to determine the genetic basis for its carbapenem resistance phenotype. This clinical strain was recovered from a patient that received, one year ago, ertapenem to treat a cholangitis due to a carbapenem-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolate. Whole-genome sequencing of these strains was performed using Illumina and single molecule real-time sequencing technologies. It revealed that they belonged to the ST131 clonal group, had the predicted O25b:H4 serotype, and produced the CTX-M-15 and TEM-1 β-lactamases...
March 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28257811/functional-interaction-of-pkca-and-pldb-regulate-aggregation-and-development-in-dictyostelium-discoideum
#7
Sean Singh, Wasima Mohamed, Annelie Aguessy, Ella Dyett, Shriraj Shah, Mohammedasad Khan, Ramamurthy Baskar, Derrick Brazill
Multicellular development in Dictyostelium discoideum involves tightly regulated signaling events controlling the entry into development, initiation of aggregation and chemotaxis, and cellular differentiation. Here we show that PkcA, a Dictyostelium discoideum Protein Kinase C-orthologue, is involved in quorum sensing and the initiation of development, as well as cAMP sensing during chemotaxis. Additionally, by epistasis analysis we provide evidence that PkcA and PldB (a Phospholipase D-orthologue) functionally interact to regulate aggregation, differentiation, and cell-cell adhesion during development...
February 28, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28256720/a-unique-high-throughput-assay-to-identify-novel-small-molecule-inhibitors-of-chemotaxis-and-migration
#8
Xin-Hua Liao, Alan R Kimmel
Chemotaxis and cell migration play pivotal roles in normal physiological processes such as embryogenesis, inflammation, and wound healing, as well as in pathological processes including chronic inflammatory disease and cancer metastasis. Novel chemotaxis/migration inhibitors are desirable for developing effective therapeutics and probing molecular mechanisms. We describe a fluorescence-based phenotypic assay in a 1536-well plate format for high-throughput screening of novel inhibitors of chemotaxis/migration within complex libraries of thousands of compounds...
March 3, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28252050/site-specific-adp-ribosylation-of-histone-h2b-in-response-to-dna-double-strand-breaks
#9
Alina Rakhimova, Seiji Ura, Duen-Wei Hsu, Hong-Yu Wang, Catherine J Pears, Nicholas D Lakin
ADP-ribosyltransferases (ARTs) modify proteins with single units or polymers of ADP-ribose to regulate DNA repair. However, the substrates for these enzymes are ill-defined. For example, although histones are modified by ARTs, the sites on these proteins ADP-ribosylated following DNA damage and the ARTs that catalyse these events are unknown. This, in part, is due to the lack of a eukaryotic model that contains ARTs, in addition to histone genes that can be manipulated to assess ADP-ribosylation events in vivo...
March 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28239623/isolation-of-latex-bead-phagosomes-from-dictyostelium-for-in-vitro-functional-assays
#10
Ashwin D'Souza, Paulomi Sanghavi, Ashim Rai, Divya Pathak, Roop Mallik
We describe a protocol to purify latex bead phagosomes (LBPs) from Dictyostelium cells. These can be later used for various in vitro functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on in vitro polymerized microtubules. Phagosomes are allowed to mature for defined periods inside cells before extraction for in vitro motility. These assays allow us to probe how lipids on the phagosome membrane recruit and organize motors, and also measure the motion and force generation resulting from underlying lipid-motor interactions...
December 5, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28211207/social-amoebae-mating-types-do-not-invest-unequally-in-sexual-offspring
#11
Tracy E Douglas, David C Queller, Joan E Strassmann
Unequal investment by different sexes in their progeny is common and includes differential investment in the zygote and differential care of the young. The social amoeba Dictyostelium discoideum has a sexual stage in which isogamous cells of any two of the three mating types fuse to form a zygote which then attracts hundreds of other cells to the macrocyst. The latter cells are cannibalized and so make no genetic contribution to reproduction. Previous literature suggests that this sacrifice may be induced in cells of one mating type by cells of another, resulting in a higher than expected production of macrocysts when the inducing type is rare and a giving a reproductive advantage to this social cheat...
February 17, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/28210928/bacterial-resistance-to-arsenic-protects-against-protist-killing
#12
Xiuli Hao, Xuanji Li, Chandan Pal, Jon Hobman, D G Joakim Larsson, Quaiser Saquib, Hend A Alwathnani, Barry P Rosen, Yong-Guan Zhu, Christopher Rensing
Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyostelium discoideum (D. discoideum). Deletion of the E. coli ars operon led to significantly lower intracellular survival compared to wild type E. coli. This suggests that protists use arsenic to poison bacterial cells in the phagosome, similar to their use of copper...
February 16, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28205535/phylogenetic-analysis-of-eukaryotic-neet-proteins-uncovers-a-link-between-a-key-gene-duplication-event-and-the-evolution-of-vertebrates
#13
Madhuri A Inupakutika, Soham Sengupta, Rachel Nechushtai, Patricia A Jennings, Jose' N Onuchic, Rajeev K Azad, Pamela Padilla, Ron Mittler
NEET proteins belong to a unique family of iron-sulfur proteins in which the 2Fe-2S cluster is coordinated by a CDGSH domain that is followed by the "NEET" motif. They are involved in the regulation of iron and reactive oxygen metabolism, and have been associated with the progression of diabetes, cancer, aging and neurodegenerative diseases. Despite their important biological functions, the evolution and diversification of eukaryotic NEET proteins are largely unknown. Here we used the three members of the human NEET protein family (CISD1, mitoNEET; CISD2, NAF-1 or Miner 1; and CISD3, Miner2) as our guides to conduct a phylogenetic analysis of eukaryotic NEET proteins and their evolution...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28186323/the-arp2-3-inhibitory-protein-arpin-is-dispensable-for-chemotaxis
#14
Irène Dang, Joern Linkner, Jun Yan, Daniel Irimia, Jan Faix, Alexis Gautreau
Arpin is an Arp2/3 inhibitory protein, which decreases the protrusion lifetime and hence directional persistence in the migration of diverse cells. Arpin is activated by the small GTPase Rac, which controls cell protrusion, thus closing a negative feedback loop that renders the protrusion intrinsically unstable. Because of these properties, it was proposed that Arpin might play a role in directed migration, where directional persistence has to be fine-tuned. We report here, however, that Arpin depleted tumor cells and Arpin knock-out Dictyostelium amoeba display no obvious defect in chemotaxis...
February 10, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28183814/comparative-proteomics-of-purified-pathogen-vacuoles-correlates-intracellular-replication-of-legionella-pneumophila-with-the-small-gtpase-rap1
#15
Johanna Schm Oumllders, Christian Manske, Andreas Otto, Christine Hoffmann, Bernhard Steiner, Amanda Welin, D Oumlrte Becher, Hubert Hilbi
Legionella pneumophila is an opportunistic bacterial pathogen that causes a severe lung infection termed Legionnaires disease. The pathogen replicates in environmental protozoa as well as in macrophages within a unique membrane-bound compartment, the Legionella-containing-vacuole (LCV). LCV formation requires the bacterial Icm/Dot type IV secretion system, which translocates ca. 300 effector proteins into host cells, where they target distinct host factors. The L. pneumophila pentuple mutant (Δpentuple) lacks 5 gene clusters (31% of the effector proteins) and replicates in macrophages but not in Dictyostelium discoideum amoeba...
February 9, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28158557/effects-of-deletion-of-the-receptor-crla-on-dictyostelium-aggregation-and-mpbd-mediated-responses-are-strain-dependent-and-not-evident-in-strain-ax2
#16
Takaaki B Narita, Pauline Schaap, Tamao Saito
No abstract text is available yet for this article.
February 2, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28136018/co-existence-of-ras-activation-in-a-chemotactic-signal-transduction-pathway-and-in-an-autonomous-wave-forming-system
#17
Mary Ecke, Günther Gerisch
The activation of Ras is common to two activities in cells of Dictyostelium discoideum: the directed movement in a gradient of chemoattractant and the autonomous generation of propagating waves of actin polymerization on the substrate-attached cell surface. We produced large cells by electric-pulse induced fusion to simultaneously study both activities in one cell. For imaging, a fluorescent label for activated Ras was combined with labels for filamentous actin, PIP3, or PTEN. Chemotactic responses were elicited in a diffusion gradient of cyclic AMP...
January 31, 2017: Small GTPases
https://www.readbyqxmd.com/read/28128835/identification-of-novel-inhibitors-of-the-translationally-controlled-tumor-protein-tctp-insights-from-molecular-dynamics
#18
Rakesh Kumar, Ranjana Maurya, Shweta Saran
The translationally controlled tumor protein (TCTP) is a highly conserved multifunctional protein, preferentially expressed in mitotically active tissues and is a potential biomarker and a therapeutic target for lung cancers. An understanding of the biology of this molecule and model systems for the screening of drugs is still awaited. In the absence of complete crystal structure, NMR structures as templates were used for homology modeling and MD optimization of both Dictyostelium discoideum and human TCTPs, which was followed by pocket-site prediction, ligand screening and docking...
January 27, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28124777/minimal-amino-acids-in-the-i-lweq-domain-required-for-anterior-posterior-localization-in-dictyostelium
#19
Hyeseon Kim, Dong-Yeop Shin, Taeck Joong Jeon
Establishment of cell polarity is mediated by a series of signaling molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localization could, with some additional deletion at the C-terminal, localize to the anterior...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28122819/method-to-study-cell-migration-under-uniaxial-compression
#20
Nishit Srivastava, Robert R Kay, Alexandre J Kabla
The chemical, physical and mechanical properties of the extra-cellular environment have a strong effect on cell migration. Aspects such as pore-size or stiffness of the matrix influence the selection of the mechanism used by cells to propel themselves, including pseudopod or blebbing. How a cell perceives its environment, and how such a cue triggers a change in behaviour are largely unknown, but mechanics is likely to be involved. Because mechanical conditions are often controlled by modifying the composition of the environment, separating chemical and physical contributions is difficult and requires multiple controls...
January 25, 2017: Molecular Biology of the Cell
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