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https://www.readbyqxmd.com/read/29054743/simultaneous-rna-seq-based-transcriptional-profiling-of-intracellular-brucella-abortus-and-b-abortus-infected-murine-macrophages
#1
Huynh Tan Hop, Lauren Togonon Arayan, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, WonGi Min, Hu Jang Lee, Jee Soo Son, Suk Kim
Brucella is a zoonotic pathogen that survives within macrophages; however the replicative mechanisms involved are not fully understood. We describe the isolation of sufficient Brucella abortus RNA from primary host cell environment using modified reported methods for RNA-seq analysis, and simultaneously characterize the transcriptional profiles of intracellular B. abortus and bone marrow-derived macrophages (BMM) from BALB/c mice at 24 h (replicative phase) post-infection. Our results revealed that 25.12% (801/3190) and 16...
October 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29054724/proinflammatory-cytokine-interferon-%C3%AE-increases-the-expression-of-bancr-a-long-non-coding-rna-in-retinal-pigment-epithelial-cells
#2
R Krishnan Kutty, William Samuel, Todd Duncan, Olga Postnikova, Cynthia Jaworski, Chandrasekharam N Nagineni, T Michael Redmond
The inflammatory response may contribute to retinal pigment epithelial (RPE) dysfunction associated with the pathogenesis of age-related macular degeneration (AMD). We investigated whether the inflammatory response affects the expression of long coding RNAs (lncRNAs) in human RPE-derived ARPE-19 cells. This class of regulatory RNA molecules recently came to prominence due to their involvement in many pathophysiological processes. A proinflammatory cytokine mixture consisting of IFN-γ, IL-1β and TNF-α altered the expression several lncRNAs including BANCR in these cells...
October 17, 2017: Cytokine
https://www.readbyqxmd.com/read/29054602/stat1-gain-of-function-and-stat3-loss-of-function-mutations-impair-il-17-and-il-22-immunity-associated-with-cmc
#3
Julia Hiller, Beate Hagl, Renate Effner, Anne Puel, Martin Schaller, Birgit Mascher, Stefanie Eyerich, Kilian Eyerich, Annette F Jansson, Johannes Ring, Jean-Laurent Casanova, Ellen D Renner, Claudia Traidl-Hoffmann
No abstract text is available yet for this article.
October 17, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29054137/il-35-gene-modified-mesenchymal-stem-cells-protect-the-con-a-induced-fulminant-hepatitis-through-decreasing-the-ifn-g-level
#4
Wei Wang, Hao Guo, Hongyue Li, Yongjia Yan, Chao Wu, Xiaodong Wang, Xianghui He, Na Zhao
Interleukin 35 (IL-35) is a relatively newly identified cytokine required for the regulatory and suppressive functions of regulatory T cells(Treg), playing an important role in the prevention of autoimmune diseases. In this study, we used the MSCs as the gene-delivery vehicles for IL-35 gene therapy and investigated their protective effects in Con A-induced autoimmune hepatitis. Results showed that IL-35 gene modified MSCs(IL-35-MSCs) can specifically migrate to the injured liver tissues and significantly narrow the necrosis areas and reduce the hepatocytes apoptosis...
October 20, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/29051763/il-12p35-inhibits-neuroinflammation-and-ameliorates-autoimmune-encephalomyelitis
#5
Jin Kyeong Choi, Ivy M Dambuza, Chang He, Cheng-Rong Yu, Anita N Uche, Mary J Mattapallil, Rachel R Caspi, Charles E Egwuagu
Multiple sclerosis (MS) is an inflammatory demyelinating disease in which cytokines produced by immune cells that infiltrate the brain and spinal cord play a central role. We show here that the IL-12p35, the alpha subunit of IL-12 or IL-35 cytokine, might be an effective biologic for suppressing neuroinflammatory responses and ameliorating the pathology of experimental autoimmune encephalomyelitis (EAE), the mouse model of human MS. We further show that IL-12p35 conferred protection from neuropathy by inhibiting the expansion of pathogenic Th17 and Th1 cells and inhibiting trafficking of inflammatory cells into the brain and spinal cord...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29050978/chrysin-attenuates-atopic-dermatitis-by-suppressing-inflammation-of-keratinocytes
#6
Jin Kyeong Choi, Yong Hyun Jang, Soyoung Lee, Sang-Rae Lee, Young-Ae Choi, Meiling Jin, Jung Ho Choi, Jee Hun Park, Pil-Hoon Park, Hyukjae Choi, Taeg Kyu Kwon, Dongwoo Khang, Sang-Hyun Kim
We previously reported the inhibitory effect of chrysin, a natural flavonoid plentifully contained in propolis, vegetables and fruits, on the mast cell-mediated allergic reaction. In this study, we evaluated the effect of chrysin on atopic dermatitis (AD) and defined underlying mechanisms of action. We used an AD model in BALB/c mice by the repeated local exposure of 2,4-dinitrochlorobenzene (DNCB) and house dust mite (Dermatophagoides farinae extract, DFE) to the ears. Repeated alternative treatment of DNCB/DFE caused AD-like skin lesions...
October 16, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29050862/jak-stat-signaling-pathway-gene-expression-is-reduced-following-nelf-knockdown-in-gnrh-neurons
#7
Eun Kyung Ko, Lynn P Chorich, Megan E Sullivan, Richard S Cameron, Lawrence C Layman
Hypothalamic gonadotropin releasing hormone (GnRH) is crucial for the proper function of the hypothalamic-pituitary-gonadal (HPG) axis, subsequent puberty, and reproduction. When GnRH neuron migration or GnRH regulation is impaired, hypogonadotropic hypogonadism results. Mutations in the gene for nasal embryonic luteinizing hormone-releasing factor (NELF) have been identified in GnRH-deficient humans. NELF is a predominantly nuclear protein that may participate in gene transcription, but the genes NELF regulates are unknown...
October 16, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29050480/the-effect-of-leptin-and-resveratrol-on-jak-stat-pathways-and-sirt-1-gene-expression-in-the-renal-tissue-of-ischemia-reperfusion-induced-rats
#8
S Erkasap, N Erkasap, B Bradford, L Mamedova, O Uysal, M Ozkurt, R Ozyurt, O Kutlay, B Bayram
OBJECTIVE: Our study aimed to investigate the possible modifying effects of leptin and combined use of resveratrol on rat renal I/R injury and their relationship on signal pathways and apoptosis-related mechanisms. BACKGROUND: Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure. METHODS: Male Sprague Dawley rats were divided into 5 groups: Control, I/R, I/R+leptin, I/R+resveratrol and I/R+leptin+resveratrol...
2017: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29048616/the-receptor-for-activated-protein-kinase-c-promotes-cell-growth-invasion-and-migration-in-cervical-cancer
#9
Shan Liao, Songshu Xiao, Hongxiang Chen, Manying Zhang, Zhifang Chen, Yuehua Long, Lu Gao, Junyu He, Yanshan Ge, Wei Yi, Minghua Wu, Guiyuan Li, Yanhong Zhou
Cervical cancer is one of the most common malignant tumors in women all over the world. However, the exact etiology of cervical cancer remains unclear. The receptor for activated protein kinase C (RACK1) is reported to be involved in tumorigenesis and tumor progression. Besides, the prognostic value of RACK1 in several kinds of tumors has been identified. However, there are limited studies on the functional role of RACK1 in cervical cancer. In this study, we tested the expression level of RACK1 by immunohistochemistry and western blot technologies and find that it is upregulated in cervical cancer...
September 27, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29042669/rna-editing-by-adar1-regulates-innate-and-antiviral-immune-functions-in-primary-macrophages
#10
Maria Pujantell, Eva Riveira-Muñoz, Roger Badia, Marc Castellví, Edurne Garcia-Vidal, Guillem Sirera, Teresa Puig, Cristina Ramirez, Bonaventura Clotet, José A Esté, Ester Ballana
ADAR1-dependent A-to-I editing has recently been recognized as a key process for marking dsRNA as self, therefore, preventing innate immune activation and affecting the development and resolution of immune-mediated diseases and infections. Here, we have determined the role of ADAR1 as a regulator of innate immune activation and modifier of viral susceptibility in primary myeloid and lymphoid cells. We show that ADAR1 knockdown significantly enhanced interferon, cytokine and chemokine production in primary macrophages that function as antiviral paracrine factors, rendering them resistant to HIV-1 infection...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29040919/functional-analyses-of-the-interaction-of-chicken-interleukin-23-subunit-p19-with-il-12-subunit-p40-to-form-the-il-23-complex
#11
Anh Duc Truong, Cong Thanh Hoang, Yeojin Hong, Janggeun Lee, Kyungbaek Lee, Hyun S Lillehoj, Yeong Ho Hong
This study represents the first description of the cloning of chicken IL-23p19 (ChIL-23α) and the function of the IL-23 complex in birds. Multiple alignment of ChIL-23α with other known IL-23α amino acid sequences revealed regions of amino acid conservation. The homologies of ChIL-23α, IL-12p35, and similar mammalian subunits ranged between 26% and 42%. ChIL-23α consisted of four exons and three introns; similar to those in humans and mice, and limited conservation of synteny between the human and chicken genomes was observed...
October 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29040650/interferon-alpha-reduces-human-hippocampal-neurogenesis-and-increases-apoptosis-via-activation-of-distinct-stat1-dependent-mechanisms
#12
Alessandra Borsini, Annamaria Cattaneo, Chiara Malpighi, Sandrine Thuret, Neil A Harrison, Patricia A Zunszain, Carmine M Pariante
Background: In humans, interferon-α treatment for chronic viral hepatitis is a well-recognized clinical model for inflammation-induced depression, but the molecular mechanisms underlying these effects are not clear. Following peripheral administration in rodents, interferon-α induces signal transducer and activator of transcription-1 (STAT1) within the hippocampus and disrupts hippocampal neurogenesis. Methods: We used the human hippocampal progenitor cell line HPC0A07/03C to evaluate the effects of 2 concentrations of interferon-α, similar to those observed in human serum during its therapeutic use (500 pg/mL and 5000 pg/mL), on neurogenesis and apoptosis...
October 10, 2017: International Journal of Neuropsychopharmacology
https://www.readbyqxmd.com/read/29039521/antitumoral-effect-of-arsenic-compound-sodium-metaarsenite-kml001-on-multiple-myeloma-cells
#13
Seo Ju Kim, Eun Shil Kim, Sujong Kim, Jieun Uhm, Young Woong Won, Byeong Bae Park, Jung Hye Choi, Young Yiul Lee
KML001 (sodium metaarsenite;NaAs2O3) is known to have antitumor activity against a variety of cancers. In this study, we examined its effect on multiple myeloma (MM). KML001 reduced the growth of all MM cell lines examined with an IC50 of 5x10‑8 M. Exposure to KML001 (5x10‑8 M) decreased levels of cyclins A/B1/D1/E1, CDK2/4/6 in U266 cells and increased the p21 and p27 levels. Furthermore, p21 became bound to CDK2/4/6, resulting in a reduction of CDK2/4/6 kinase activity. The cleaved forms of Bcl-2, and caspases‑3, ‑8 and ‑9 were detected, and the anti-apoptotic molecule, Bax, also increased...
October 16, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29033950/glutathione-fine-tunes-the-innate-immune-response-toward-antiviral-pathways-in-a-macrophage-cell-line-independently-of-its-antioxidant-properties
#14
Marina Diotallevi, Paola Checconi, Anna Teresa Palamara, Ignacio Celestino, Lucia Coppo, Arne Holmgren, Kahina Abbas, Fabienne Peyrot, Manuela Mengozzi, Pietro Ghezzi
Glutathione (GSH), a major cellular antioxidant, is considered an inhibitor of the inflammatory response involving reactive oxygen species (ROS). However, evidence is largely based on experiments with exogenously added antioxidants/reducing agents or pro-oxidants. We show that depleting macrophages of 99% of GSH does not exacerbate the inflammatory gene expression profile in the RAW264 macrophage cell line or increase expression of inflammatory cytokines in response to the toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS); only two small patterns of LPS-induced genes were sensitive to GSH depletion...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29033931/plasmodium-berghei-nk65-in-combination-with-ifn-%C3%AE-induces-endothelial-glucocorticoid-resistance-via-sustained-activation-of-p38-and-jnk
#15
Karolina A Zielińska, Lode de Cauwer, Sofie Knoops, Kristof Van der Molen, Alexander Sneyers, Jonathan Thommis, J Brian De Souza, Ghislain Opdenakker, Karolien De Bosscher, Philippe E Van den Steen
Malaria-associated acute respiratory distress syndrome (MA-ARDS) is an often lethal complication of malaria. Currently, no adequate therapy for this syndrome exists. Although glucocorticoids (GCs) have been used to improve clinical outcome of ARDS, their therapeutic benefits remain unclear. We previously developed a mouse model of MA-ARDS, in which dexamethasone treatment revealed GC resistance. In the present study, we investigated GC sensitivity of mouse microvascular lung endothelial cells stimulated with interferon-γ (IFN-γ) and Plasmodium berghei NK65 (PbNK65)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29033545/downregulation-of-a20-increases-the-cytotoxicity-of-ifn-%C3%AE-in-hepatocellular-carcinoma-cells
#16
Lei Yin, Zheng Fang, Ning-Jia Shen, Ying-He Qiu, Ai-Jun Li, Yong-Jie Zhang
Hepatocellular carcinoma (HCC) is a highly fatal disease mandating development of novel, effective therapeutic strategy. Interferon-gamma (IFN-γ) is a pleiotropic cytokine with immunomodulatory, antiviral, and antitumor effects. Although IFN-γ is a promising antitumor agent, its application is limited by resistance in tumor cells. A20 is a zinc-finger protein that was initially identified as a gene product induced by tumor necrosis factor α in human umbilical vein endothelial cells. In this study, we found that silencing of A20 combined with IFN-γ significantly represses cell viability, and induces apoptosis and cell-cycle arrest in HCC cells...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29031523/6-hydroxy-3-o-methyl-kaempferol-6-o-glucopyranoside-potentiates-the-anti-proliferative-effect-of-interferon-%C3%AE-%C3%AE-by-promoting-activation-of-the-jak-stat-signaling-by-inhibiting-socs3-in-hepatocellular-carcinoma-cells
#17
Orawan Wonganan, Yu-Jiao He, Xiao-Fei Shen, Kanjana Wongkrajang, Apichart Suksamrarn, Guo-Lin Zhang, Fei Wang
Suppressor of cytokine signaling 3 (SOCS3) is a key negative regulator of type I interferon (IFN α/β) signaling. Inhibition of SOCS3 by small molecules may be a new strategy to enhance the efficacy of type I IFN and reduce its side effects. We established a cell-based screening assay using human hepatoma HepG2 cells stably transfected with a plasmid wherein the luciferase reporter activity was propelled by interferon α-stimulated response element (ISRE), which is a motif specifically recognized by type I IFN-induced activation of Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway...
October 12, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29031499/micrornas-15a-and-16-1-activate-signaling-pathways-that-mediate-chemotaxis-of-immune-regulatory-b-cells-to-colorectal-tumors
#18
Ronghua Liu, Zhou Lu, Jie Gu, Jiajing Liu, Enyu Huang, Xiaoming Liu, Luman Wang, Jiao Yang, Yuting Deng, Jiawen Qian, Feifei Luo, Zhiming Wang, Hushan Zhang, Xuechao Jiang, Dan Zhang, Jing Qian, Guangwei Liu, Hongguang Zhu, Youcun Qian, Zhanju Liu, Yiwei Chu
BACKGROUND & AIMS: B cells infiltrate tumors but little is known about how they affect tumor growth and progression. microRNA15A (MIR15A or miRNA15A) and microRNA16-1 (MIR16-1 or miRNA16-1) regulate cell proliferation, apoptosis and drug resistance. We investigated their involvement in B cell-mediated immune suppression by colorectal tumors. METHODS: Mice with disruptions of the gene cluster that encodes MIR15A and MIR16-1 (KO mice), and control (C57BL/B6) mice were given azoxymethane with dextran sodium sulfate (AD) to induce formation of colorectal tumors...
October 11, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29029022/functional-characterization-of-microrna-27a-3p-expression-in-human-polycystic-ovary-syndrome
#19
Mingming Wang, Jing Sun, Bo Xu, Marcin Chrusciel, Jun Gao, Maciei Bazert, Joanna Stelmaszewska, Yunyun Xu, Hongwen Zhang, Leszek Pawelczyk, Fei Sun, Nafis Rahman, Slawomir Wolczynski, Suk Ying Tsang, Xiangdong Li
The goal of this study was to characterize the function of microRNA-27a-3p (miR-27a-3p) in polycystic ovary syndrome (PCOS). MiR-27a-3p expression was analyzed in excised granulosa cells (GCs) from 21 PCOS and 12 normal patients undergoing IVF cycle treatments, and in 17 non-treated cuneiform ovarian resection PCOS samples and 13 control ovarian samples-from non-PCOS patients. We found that the expression of miR-27a-3p was significantly increased in both excised GCs and the ovaries of PCOS patients compared to the controls...
September 29, 2017: Endocrinology
https://www.readbyqxmd.com/read/29028431/interferon-tau-regulates-cytokine-production-and-cellular-function-in-human-trophoblast-cell-line
#20
Nao Tanikawa, Kotomi Seno, Ryouka Kawahara-Miki, Koji Kimura, Shuichi Matsuyama, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
Type I interferons (IFN), including IFN-beta (IFNB), activate multiple STAT signaling to drive various biological responses. Another type I IFN, IFN-tau (IFNT), secreted by ruminant embryonic trophoblast cells, has multiple functions with low cytotoxicity. Here, we examined the effects of IFNT on human trophoblast cell functions. First, we performed next-generation sequencing and demonstrated that IFNT-dependent changes in the human Sw.71 trophoblast cell line are partly mediated by proinflammatory as well as IFN signaling...
October 2017: Journal of Interferon & Cytokine Research
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