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Recombinant hsp70

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https://www.readbyqxmd.com/read/29738871/identification-of-litopenaeus-vannamei-bip-as-a-novel-cellular-attachment-protein-for-white-spot-syndrome-virus-by-using-a-biotinylation-based-affinity-chromatography-method
#1
Zengzhi Yuan, Meng Chen, Jingting Wang, Zhuoyu Li, Xuyun Geng, Jinsheng Sun
White spot syndrome virus (WSSV) is a dangerous threat to shrimp farming that also attacks a wide range of crustaceans. Knowledge of the surface protein-protein interactions between the pathogen and host is very crucial to unraveling the molecular pathogenesis mechanisms of WSSV. In this study, LvBiP (Litopenaeus vannamei immunoglobulin heavy-chain-binding protein) was identified as a novel WSSV binding protein of L. vannamei by a biotinylation based affinity chromatography method. By using pull-down and ELISA assays, the binding of recombinant LvBiP to WSSV was proved to be specific and ATP- dependent...
May 5, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29601993/antimicrobial-activity-of-a-serine-proteinase-inhibitor-spipm5-from-the-black-tiger-shrimp-penaeus-monodon
#2
Sittichai Boonrawd, Premruethai Supungul, Anchalee Tassanakajon, Vichien Rimphanitchayakit
A two-domain Kazal-type serine proteinase inhibitor, SPIPm5, from Penaeus monodon was studied. Its transcript was expressed in all tissues tested including the hemocytes, stomach, gill, lymphoid organ, muscle, intestine and heart albeit less in hepatopancreas and eyestalk. The expression of SPIPm5 gene was also up-regulated by heat stress, white spot syndrome virus (WSSV) infection and yellow head virus (YHV) infection. Injection of recombinant rSPIPm5 protein into normal shrimp to mimic heat stress condition did not have or had little stimulating effect on the expression of other immune genes: crustinPm1, penaeidin3, penaeidin5, Hsp70, SPIPm2 and SPIPm5...
March 27, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29483524/inhibition-of-heat-shock-proteins-hsp90-and-hsp70-induce-oxidative-stress-suppressing-cotton-fiber-development
#3
Anshulika Sable, Krishan M Rai, Amit Choudhary, Vikash K Yadav, Sudhir K Agarwal, Samir V Sawant
Cotton fiber is a specialized unicellular structure useful for the study of cellular differentiation and development. Heat shock proteins (HSPs) have been shown to be involved in various developmental processes. Microarray data analysis of five Gossypium hirsutum genotypes revealed high transcript levels of GhHSP90 and GhHSP70 genes at different stages of fiber development, indicating their importance in the process. Further, we identified 26 and 55 members of HSP90 and HSP70 gene families in G. hirsutum. The treatment of specific inhibitors novobiocin (Nov; HSP90) and pifithrin/2-phenylethynesulfonamide (Pif; HSP70) in in-vitro cultured ovules resulted in a fewer number of fiber initials and retardation in fiber elongation...
February 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29435724/transcriptome-expression-and-activity-analyses-reveal-a-vital-heat-shock-protein-70-in-the-stress-response-of-stony-coral-pocillopora-damicornis
#4
Yidan Zhang, Zhi Zhou, Lingui Wang, Bo Huang
Coral bleaching occurs worldwide with increasing frequencies and intensities, which is caused by the stress response of stony coral to environmental change, especially increased sea surface temperature. In the present study, transcriptome, expression, and activity analyses were employed to illustrate the underlying molecular mechanisms of heat shock protein 70 (HSP70) in the stress response of coral to environmental changes. The domain analyses of assembled transcripts revealed 30 HSP70 gene contigs in stony coral Pocillopora damicornis...
February 12, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29374537/hsp70-facilitates-memory-consolidation-of-fear-conditioning-through-mapk-pathway-in-the-hippocampus
#5
Rossana R Porto, Fabrício D Dutra, Ana Paula Crestani, R M Damian Holsinger, Jorge A Quillfeldt, Paulo Ivo Homem de Bittencourt, Lucas de Oliveira Alvares
Heat shock proteins of the 70-kDa (HSP70) family are cytoprotective molecular chaperones that are present in neuronal cells and can be induced by a variety of homeostatically stressful situations (not only proteostatic insults), but also by synaptic activity, including learning tasks. Physiological stimuli that induce long-term memory formation are also capable of stimulating the synthesis of HSP70 through the activation of heat shock transcription factor-1 (HSF1). In this study, we investigated the influence of HSP70 on fear memory consolidation and MAPK activity...
April 1, 2018: Neuroscience
https://www.readbyqxmd.com/read/29206141/-epigallocatechin-3-gallate-inhibits-the-chaperone-activity-of-plasmodium-falciparum-hsp70-chaperones-and-abrogates-their-association-with-functional-partners
#6
Tawanda Zininga, Lebogang Ramatsui, Pertunia Bveledzani Makhado, Stanley Makumire, Ikechukwu Achilinou, Heinrich Hoppe, Heini Dirr, Addmore Shonhai
Heat shock proteins (Hsps), amongst them, Hsp70 and Hsp90 families, serve mainly as facilitators of protein folding (molecular chaperones) of the cell. The Hsp70 family of proteins represents one of the most important molecular chaperones in the cell. Plasmodium falciparum , the main agent of malaria, expresses six Hsp70 isoforms. Two (PfHsp70-1 and PfHsp70-z) of these localize to the parasite cytosol. PHsp70-1 is known to occur in a functional complex with another chaperone, PfHsp90 via a co-chaperone, P. falciparum Hsp70-Hsp90 organising protein (PfHop)...
December 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29203716/immune-tolerance-therapies-for-autoimmune-diseases-based-on-heat-shock-protein-t-cell-epitopes
#7
REVIEW
Willem van Eden
Experimental models of autoimmune diseases have revealed the disease protective role of heat shock proteins (HSPs). Both the administration of exogenous extracellular, mostly recombinant, HSP and the experimental co-induction of endogenous intracellular HSP in models have been shown to lead to production of disease protective regulatory T cells (Tregs). Similar to HSP taken up from extracellular bodily fluids, due to stress-related autophagy upregulated HSP also from intracellular sources is a major provider for the major histocompatibility class II (MHCII) ligandome; therefore, both extracellular and intracellular HSP can be prominent targets of Treg...
January 19, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29170749/-in-vitro-ampylation-assays-using-purified-recombinant-proteins
#8
Matthias C Truttmann, Hidde L Ploegh
Post-translational protein modifications (PTMs) orchestrate the activity of individual proteins and ensure their proper function. While modifications such as phosphorylation or glycosylation are well understood, more unusual modifications, including nitrosylation or AMPylation remain comparatively poorly characterized. Research on protein AMPylation-which refers to the covalent addition of an AMP moiety to the side chains of serine, threonine or tyrosine-has undergone a renaissance (Yarbrough et al ., 2009; Engel et al ...
July 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/29140786/selection-of-an-anticalin%C3%A2-against-the-membrane-form-of-hsp70-via-bacterial-surface-display-and-its-theranostic-application-in-tumour-models
#9
Lars Friedrich, Petra Kornberger, Claudia T Mendler, Gabriele Multhoff, Markus Schwaiger, Arne Skerra
We describe the selection of Anticalins against a common tumour surface antigen, human Hsp70, using functional display on live Escherichia coli cells as fusion with a truncated EspP autotransporter. While found intracellularly in normal cells, Hsp70 is frequently exposed in a membrane-bound state on the surface of tumour cells and, even more pronounced, in metastases or after radiochemotherapy. Employing a recombinant Hsp70 fragment comprising residues 383-548 as the target, Anticalins were selected from a naïve bacterial library...
February 23, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29127260/an-improved-brome-mosaic-virus-silencing-vector-greater-insert-stability-and-more-extensive-vigs
#10
Xin Shun Ding, Stephen W Mannas, Bethany A Bishop, Xiaolan Rao, Mitchell Lecoultre, Soonil Kwon, Richard S Nelson
Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient phenotypes is the instability of the foreign gene fragment (insert) in the vector during VIGS. Here, we report the development of a Brome mosaic virus (BMV)-based vector that better maintains inserts through modification of the original BMV vector RNA sequence...
January 2018: Plant Physiology
https://www.readbyqxmd.com/read/29107750/molecular-cloning-prokaryotic-expression-purification-structural-studies-and-functional-implications-of-heat-shock-protein-70-hsp70-from-rutilus-frisii-kutum
#11
Zohreh Jahangirizadeh, Hossein Ghafouri, Reza H Sajedi, Sajjad Sarikhan, Majid Taghdir, Reyhaneh Sariri
A novel Hsp70 chaperone from Rutilus frisii kutum was identified, cloned, expressed, purified and its functional characteristics revealed. The 3D structure of Hsp70 from Rutilus kutum was constructed using the crystal structure of E. coli Hsp70 as the template, with 47% sequence identity. The in vitro ATPase activity assay after 60min, ATP hydrolysis of purified recombinant Hsp70 (8μM) was improved by binding to denatured thermally luciferase (3μM) about 2.5-fold compared with that of Hsp70 alone. Based on the results, it was found that the purified Hsp70 chaperone was able to considerably suppress heat-induced aggregation of luciferase by binding to DnaJ co-chaperone (5μM) more than 70% after 10min at 42°C...
March 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29102627/the-anti-stresses-capability-of-grp78-in-penaeus-monodon-evidence-from-in-vitro-and-in-vivo-studies
#12
Lili Li, Pengfei Wang, Chao Zhao, Lihua Qiu
Glucose-regulated protein 78 (GRP78) is an important member of the heat shock protein 70 (HSP70) family, which plays important roles in the response to stimulation from various external environmental stimuli. In this study, the full-length cDNA of GRP78 (PmGRP78) was cloned from Penaeus monodon. The qRT-PCR analysis showed that PmGRP78 was ubiquitously expressed in all the tested shrimp tissues, and the expression pattern of PmGRP78 in the gill and hepatopancreas tissues after a pH challenge, osmotic stress, and heavy metal exposure was also detected...
January 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29067554/extracellular-hsp70-induces-inflammation-and-modulates-lps-lta-stimulated-inflammatory-response-in-thp-1-cells
#13
Andrea Hulina, Marija Grdić Rajković, Daniela Jakšić Despot, Dubravko Jelić, Ana Dojder, Ivana Čepelak, Lada Rumora
Extracellular Hsp70 (eHsp70) can act as damage-associated molecular pattern (DAMP) via Toll-like receptors TLR2 and TLR4, and stimulate immune and inflammatory responses leading to sterile inflammation and propagation of already existing inflammation. It was found elevated in the blood of patients with chronic obstructive pulmonary disease (COPD), who might suffer occasional bacterial colonizations and infections. We used a monocytic THP-1 cell line as a cellular model of systemic compartment of COPD to assess inflammatory effects of eHsp70 when present alone or together with bacterial products lypopolysaccharide (LPS) and lypoteichoic acid (LTA)...
October 24, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28951871/recombinant-vaccinia-viruses-coding-transgenes-of-apoptosis-inducing-proteins-enhance-apoptosis-but-not-immunogenicity-of-infected-tumor-cells
#14
Olga Koval, Galina Kochneva, Anastasiya Tkachenko, Olga Troitskaya, Galina Sivolobova, Antonina Grazhdantseva, Anna Nushtaeva, Elena Kuligina, Vladimir Richter
Genetic modifications of the oncolytic vaccinia virus (VV) improve selective tumor cell infection and death, as well as activation of antitumor immunity. We have engineered a double recombinant VV, coding human GM-CSF, and apoptosis-inducing protein apoptin (VV-GMCSF-Apo) for comparing with the earlier constructed double recombinant VV-GMCSF-Lact, coding another apoptosis-inducing protein, lactaptin, which activated different cell death pathways than apoptin. We showed that both these recombinant VVs more considerably activated a set of critical apoptosis markers in infected cells than the recombinant VV coding GM-CSF alone (VV-GMCSF-dGF): these were phosphatidylserine externalization, caspase-3 and caspase-7 activation, DNA fragmentation, and upregulation of proapoptotic protein BAX...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28948857/neuroprotective-effect-of-secreted-factors-from-human-adipose-stem-cells-in-a-rat-stroke-model
#15
Han Gil Seo, Youbin Yi, Byung-Mo Oh, Nam-Jong Paik
Objectives Recent evidence shows that stem cells exert neuroprotective effect through the secretion of immune modulatory, neurotrophic factors. We aimed to assess the neuroprotective effect of selected recombinant factors (RFs) detected in human adipose stem cell (hASC)-conditioned medium (CM), in a rat ischemic stroke model. Methods Ischemic stroke was induced in Sprague-Dawley rats using 2 h transient middle cerebral artery occlusion (MCAO). One hour after reperfusion, the vehicle (Dulbecco's modified Eagle medium; DMEM), concentrated CM, and selected RFs mixed with DMEM were administered intracerebroventricularly to each group (N = 14, 15, and 16, respectively)...
September 26, 2017: Neurological Research
https://www.readbyqxmd.com/read/28928431/tumor-induces-muscle-wasting-in-mice-through-releasing-extracellular-hsp70-and-hsp90
#16
Guohua Zhang, Zhelong Liu, Hui Ding, Yong Zhou, Hoang Anh Doan, Ka Wai Thomas Sin, Zhiren J Zhu, Rene Flores, Yefei Wen, Xing Gong, Qingyun Liu, Yi-Ping Li
Cachexia, characterized by muscle wasting, is a major contributor to cancer-related mortality. However, the key cachexins that mediate cancer-induced muscle wasting remain elusive. Here, we show that tumor-released extracellular Hsp70 and Hsp90 are responsible for tumor's capacity to induce muscle wasting. We detected high-level constitutive release of Hsp70 and Hsp90 associated with extracellular vesicles (EVs) from diverse cachexia-inducing tumor cells, resulting in elevated serum levels in mice. Neutralizing extracellular Hsp70/90 or silencing Hsp70/90 expression in tumor cells abrogates tumor-induced muscle catabolism and wasting in cultured myotubes and in mice...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28919581/antitumor-response-to-a-codon-optimized-hpv-16-e7-hsp70-fusion-antigen-dna-vaccine
#17
Hoorieh Soleimanjahi, Hadi Razavinikoo, Fatemeh Fotouhi, Abdollah Ardebili
BACKGROUND: Vaccines based on virus-like particles are effective against Human Papilloma Virus (HPV) infection; however, they have not shown a therapeutic effect against HPV-associated diseases. New immunotherapy strategies based on immune responses against tumor antigens can positively affect the clearance of HPV-associated lesions. OBJECTIVE: To generate two therapeutic fusion DNA vaccines (optimizedE7/mouseHSP70 and wildE7/mouseHSP70) to induce antitumor specific responses in mice models...
September 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28893913/interleukin-17a-deficient-mice-are-highly-susceptible-to-toxoplasma-gondii-infection-due-to-excessively-induced-t-gondii-hsp70-and-interferon-gamma-production
#18
Masataka Moroda, Masaya Takamoto, Yoichiro Iwakura, Jun Nakayama, Fumie Aosai
Interleukin17A (IL-17A) is known to be involved in the host defense against pathogens and the pathogenesis of autoimmune diseases. Previously, we showed that excessive amounts of interferon gamma (IFN-γ) play an important role in the pathogenesis of the lethal effects of Toxoplasma gondii by inducing anaphylactic responses. In the study described in this report, we examined the effects of IL-17A deficiency on murine host defense against oral T. gondii infection. IL-17A-deficient C57BL/6 (B6) mice exhibited higher rates of mortality than wild-type (WT) mice during the acute phase of T...
December 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28819167/artificial-dnaj-protein-for-protein-production-and-conformational-diseases
#19
Akinori Hishiya, Keizo Koya
For secreted proteins, proper protein folding is essential not only for biological function but also for secretion itself. Proteins with folding problems are trapped in the endoplasmic reticulum (ER) and are eventually degraded in the cytoplasm. In this study, we exploited co-expression of an artificial fusion protein, based on the sequence of a DnaJ protein, which could interact as co-chaperones in the Hsp70-based protein-folding system, with target recombinant secreted proteins to enhance their production and secretion...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759972/molecular-mechanisms-underlying-neuroprotective-effect-of-intranasal-administration-of-human-hsp70-in-mouse-model-of-alzheimer-s-disease
#20
Michail B Evgen'ev, George S Krasnov, Inna V Nesterova, David G Garbuz, Vadim L Karpov, Alexey V Morozov, Anastasiya V Snezhkina, Alexander N Samokhin, Alexander Sergeev, Alexei M Kulikov, Natalia V Bobkova
Heat shock protein 70, encoded by the HSPA1A gene in humans, is a key component of the machinery that protects neuronal cells from various stress conditions and whose production significantly declines during the course of aging and as a result of several neurodegenerative diseases. Herein, we investigated whether sub-chronic intranasal administration of exogenous Hsp70 (eHsp70) exerts a neuroprotective effect on the temporal cortex and areas of the hippocampus in transgenic 5XFAD mice, a model of Alzheimer's disease...
2017: Journal of Alzheimer's Disease: JAD
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