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Recombinant hsp70

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https://www.readbyqxmd.com/read/28928431/tumor-induces-muscle-wasting-in-mice-through-releasing-extracellular-hsp70-and-hsp90
#1
Guohua Zhang, Zhelong Liu, Hui Ding, Yong Zhou, Hoang Anh Doan, Ka Wai Thomas Sin, Zhiren J Zhu, Rene Flores, Yefei Wen, Xing Gong, Qingyun Liu, Yi-Ping Li
Cachexia, characterized by muscle wasting, is a major contributor to cancer-related mortality. However, the key cachexins that mediate cancer-induced muscle wasting remain elusive. Here, we show that tumor-released extracellular Hsp70 and Hsp90 are responsible for tumor's capacity to induce muscle wasting. We detected high-level constitutive release of Hsp70 and Hsp90 associated with extracellular vesicles (EVs) from diverse cachexia-inducing tumor cells, resulting in elevated serum levels in mice. Neutralizing extracellular Hsp70/90 or silencing Hsp70/90 expression in tumor cells abrogates tumor-induced muscle catabolism and wasting in cultured myotubes and in mice...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28919581/antitumor-response-to-a-codon-optimized-hpv-16-e7-hsp70-fusion-antigen-dna-vaccine
#2
Hoorieh Soleimanjahi, Hadi Razavinikoo, Fatemeh Fotouhi, Abdollah Ardebili
BACKGROUND: Vaccines based on virus-like particles are effective against Human Papilloma Virus (HPV) infection; however, they have not shown a therapeutic effect against HPV-associated diseases. New immunotherapy strategies based on immune responses against tumor antigens can positively affect the clearance of HPV-associated lesions. OBJECTIVE: To generate two therapeutic fusion DNA vaccines (optimizedE7/mouseHSP70 and wildE7/mouseHSP70) to induce antitumor specific responses in mice models...
September 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28893913/il17a-deficient-mice-are-highly-susceptible-to-toxoplasma-gondii-infection-due-to-excessively-induced-t-gondii-hsp70-and-ifn-%C3%AE-production
#3
Masataka Moroda, Masaya Takamoto, Yoichiro Iwakura, Jun Nakayama, Fumie Aosai
IL-17A is known to be involved in the host defense against pathogens and pathogenesis of autoimmune diseases. Previously, we showed that excessive IFN-γ plays an important role in the pathogenesis of lethal effect of Toxoplasma gondii (T. gondii) by inducing anaphylactic responses. In this report, we examine the effects of an IL-17A deficiency on murine host defense against oral T. gondii infection. IL-17A-deficient C57BL/6 (B6) mice exhibited higher mortality than wild type (WT) mice to T. gondii at the acute phase of infection...
September 11, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28819167/artificial-dnaj-protein-for-protein-production-and-conformational-diseases
#4
Akinori Hishiya, Keizo Koya
For secreted proteins, proper protein folding is essential not only for biological function but also for secretion itself. Proteins with folding problems are trapped in the endoplasmic reticulum (ER) and are eventually degraded in the cytoplasm. In this study, we exploited co-expression of an artificial fusion protein, based on the sequence of a DnaJ protein, which could interact as co-chaperones in the Hsp70-based protein-folding system, with target recombinant secreted proteins to enhance their production and secretion...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759972/molecular-mechanisms-underlying-neuroprotective-effect-of-intranasal-administration-of-human-hsp70-in-mouse-model-of-alzheimer-s-disease
#5
Michail B Evgen'ev, George S Krasnov, Inna V Nesterova, David G Garbuz, Vadim L Karpov, Alexey V Morozov, Anastasiya V Snezhkina, Alexander N Samokhin, Alexander Sergeev, Alexei M Kulikov, Natalia V Bobkova
Heat shock protein 70, encoded by the HSPA1A gene in humans, is a key component of the machinery that protects neuronal cells from various stress conditions and whose production significantly declines during the course of aging and as a result of several neurodegenerative diseases. Herein, we investigated whether sub-chronic intranasal administration of exogenous Hsp70 (eHsp70) exerts a neuroprotective effect on the temporal cortex and areas of the hippocampus in transgenic 5XFAD mice, a model of Alzheimer's disease...
2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28745541/chaperone-proteins-as-single-component-reagents-to-assess-antibody-nonspecificity
#6
Ryan L Kelly, James C Geoghegan, Jared Feldman, Tushar Jain, Monique Kauke, Doris Le, Jessie Zhao, K Dane Wittrup
Early stage assays that evaluate monoclonal antibody drug-like properties serve as valuable tools for selection of lead candidates. One liability for clinical development, off-target reactivity, is often assessed by binding to a mixture or panel of noncognate proteins. While robust, these mixes are often ill-defined, and can suffer from issues such as lot-to-lot variability. In this study, we discovered in immunoprecipitation experiments that certain chaperones are present in one of these mixtures; we then explored the use of recombinant chaperone proteins as well-characterized agents to predict antibody nonspecificity...
July 26, 2017: MAbs
https://www.readbyqxmd.com/read/28679534/hsp70-s-rna-binding-and-mrna-stabilizing-activities-are-independent-of-its-protein-chaperone-functions
#7
Aparna Kishor, Elizabeth J F White, Aerielle E Matsangos, Zisui Yan, Bishal Tandukar, Gerald M Wilson
Hsp70 is a protein chaperone that prevents protein aggregation and aids protein folding by binding to hydrophobic peptide domains through a reversible mechanism directed by an ATPase cycle. However, Hsp70 also binds U-rich RNA including some AU-rich elements (AREs) that regulate the decay kinetics of select mRNAs and has recently been shown to bind and stabilize some ARE-containing transcripts in cells. Previous studies indicated that both the ATP- and peptide-binding domains of Hsp70 contributed to the stability of Hsp70-RNA complexes and that ATP might inhibit RNA recruitment...
August 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28671094/expression-of-mlaa34-hsp70-fusion-gene-constructed-by-soe-pcr
#8
Chen Zhao, Jiaqi Zhao, Wanying Wang, Yening Fan, Chunping Ma, Donghong Zhang, Yang Lv
To construct the pIRES2-MLAA34-HSP70 recombinant vector and express the MLAA34-HSP70 recombinant proteins in Escherichia coli (E. coli). The MLAA34 and the HSP70 genes were extracted from U937 cells by RT-PCR, and then we amplified the fusion gene MLAA34-HSP70 by SOE-PCR and inserted it into the pIRES2-EGFP vector to construct the pIRES2-MLAA34-HSP70 recombinant vector. We amplified the fusion gene MLAA34-HSP70 successfully and identified the correctness of pIRES2-MLAA34-HSP70 recombinant vector by PCR and restriction endonuclease...
May 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28666159/optimization-of-expression-and-purification-of-human-mortalin-hsp70-folding-unfolding-analysis
#9
Mohd Shahnawaz Khan, Anwar Ahmed, Shams Tabrez, Badar Ul Islam, Nayyar Rabbani, Ajamaluddin Malik, Mohamad A Ismael, Mohammad A Alsenaidy, Abdulrahman M Alsenaidy
Human mortalin is a Hsp70 mitochondrial protein that plays an essential role in the biogenesis of mitochondria. The deregulation of mortalin expression and its functions could lead to several age-associated disorders and some types of cancers. In the present study, we optimized the expression and purification of recombinant human mortalin by the use of two-step chromatography. Low temperature (18°C) and 0.5mM (IPTG) was required for optimum mortalin expression. Chaperone activity of mortalin was assessed by the citrate synthase and insulin protection assay, which suggested their protective role in mitochondria...
June 12, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28627344/-protective-effect-of-bag-1l-mediated-by-lentivirus-in-human-neuroblastoma-cells-induced-by-hypoxia
#10
Yun Wang, Qingshu Li, Chao Jia, Chunyu Xie, Yankun Song, Nan Zhang, Yan Qu
OBJECTIVE: To investigate the protective effects of lentivirus mediated Bcl-2-associated athanogene 1L (BAG-1L) over-expression on human neuroblastoma cells (SH-SY5Y) induced by hypoxia/re-oxygenation, and to study its effect on the phosphoinositide 3 kinase serine/threonine protein kinase (PI3K/AKT) pathway. METHODS: SH-SY5Y cells were cultured in vitro, and the cells at logarithmic phase were collected, and they were divided into recombined lentiviral infection group [infected by lentivirus containing BAG-1L and green fluorescent protein (GFP) gene], vector control group (infected by lentivirus containing GFP without BAG-1L gene) and cell control group (non-infection)...
March 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28576342/development-of-a-colloidal-gold-immunochromatographic-strip-based-on-hsp70-for-the-rapid-detection-of-echinococcus-granulosus-in-sheep
#11
Xunhui Zhuo, Yingchao Yu, Xueqiu Chen, Zhuangzhi Zhang, Yi Yang, Aifang Du
Echinococcus granulosus is the causative pathogen of cystic echinococcosis, a serious disease endangering human and animal health. In this study, an immunochromatographic strip was developed based on the recombinant protein Heat shock protein 70 (HSP70) for the serological detection of E. granulosus. The protocol completes within 20min requiring no specialized equipment or chemical reagents, while specificity tests confirmed no cross-reactivity with positive serum of Fasciola hepatica, Haemonchus contortus, Peste des petits ruminants virus (PPRV) and Foot-and-mouth disease virus (FMDV)...
June 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28559404/peripheral-t-cell-reactivity-to-heat-shock-protein-70-and-its-cofactor-grpe-from-tropheryma-whipplei-is-reduced-in-patients-with-classical-whipple-s-disease
#12
Lucia Trotta, Kathleen Weigt, Katina Schinnerling, Anika Geelhaar-Karsch, Gerrit Oelkers, Federico Biagi, Gino Roberto Corazza, Kristina Allers, Thomas Schneider, Ulrike Erben, Verena Moos
Classical Whipple's disease (CWD) is characterized by the lack of specific Th1 response toward Tropheryma whipplei in genetically predisposed individuals. The cofactor GrpE of heat shock protein 70 (Hsp70) from T. whipplei was previously identified as a B-cell antigen. We tested the capacity of Hsp70 and GrpE to elicit specific proinflammatory T-cell responses. Peripheral mononuclear cells from CWD patients and healthy donors were stimulated with T. whipplei lysate or recombinant GrpE or Hsp70 before levels of CD40L, CD69, perforin, granzyme B, CD107a, and gamma interferon (IFN-γ) were determined in T cells by flow cytometry...
August 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28447215/interplay-between-recombinant-hsp70-and-proteasomes-proteasome-activity-modulation-and-ubiquitin-independent-cleavage-of-hsp70
#13
Alexey V Morozov, Tatiana M Astakhova, David G Garbuz, George S Krasnov, Natalia V Bobkova, Olga G Zatsepina, Vadim L Karpov, Michail B Evgen'ev
The heat shock protein 70 (Hsp70, human HSPA1A) plays indispensable roles in cellular stress responses and protein quality control (PQC). In the framework of PQC, it cooperates with the ubiquitin-proteasome system (UPS) to clear damaged and dysfunctional proteins in the cell. Moreover, Hsp70 itself is rapidly degraded following the recovery from stress. It was demonstrated that its fast turnover is mediated via ubiquitination and subsequent degradation by the 26S proteasome. At the same time, the effect of Hsp70 on the functional state of proteasomes has been insufficiently investigated...
April 26, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28420430/chronic-stress-induces-cd99-suppresses-autophagy-and-affects-spontaneous-adipogenesis-in-human-bone-marrow-stromal-cells
#14
Zvenyslava Husak, Michael N Dworzak
BACKGROUND: Bone marrow-derived mesenchymal stromal cells (MSCs) are multipotent cells with a high constitutive level of autophagy and low expression of CD99. Under certain conditions, MSCs may develop tumorigenic properties. However, these transformation-induced conditions are largely unknown. Recently, we have identified an association between Hsp70, a main participant in cellular stress response and tumorigenesis, and CD99. Preliminary observations had revealed upregulation of both proteins in stressed long-term cultured MSCs...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28412969/engagement-of-cellular-prion-protein-with-the-co-chaperone-hsp70-90-organizing-protein-regulates-the-proliferation-of-glioblastoma-stem-like-cells
#15
Rebeca Piatniczka Iglesia, Mariana Brandão Prado, Lilian Cruz, Vilma Regina Martins, Tiago Góss Santos, Marilene Hohmuth Lopes
BACKGROUND: Glioblastoma (GBM), a highly aggressive brain tumor, contains a subpopulation of glioblastoma stem-like cells (GSCs) that play roles in tumor maintenance, invasion, and therapeutic resistance. GSCs are therefore a promising target for GBM treatment. Our group identified the cellular prion protein (PrP(C)) and its partner, the co-chaperone Hsp70/90 organizing protein (HOP), as potential target candidates due to their role in GBM tumorigenesis and in neural stem cell maintenance...
April 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28370779/comparison-of-recombinant-proteins-of-kinesin-39-heat-shock-protein-70-heat-shock-protein-83-and-glycoprotein-63-for-antibody-detection-of-leishmania-martiniquensis-infection
#16
Suradej Siripattanapipong, Hirotomo Kato, Peerapan Tan-Ariya, Mathirut Mungthin, Saovanee Leelayoova
Leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (CL) and visceral leishmaniasis (VL) among humans and animals. This organism, firstly reported in Martinique Island, now has become an emerging infectious agent in Thailand. Symptomatic cases of L. martiniquensis infection among humans have continuously increased. In the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and control disease transmission...
March 28, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28344769/application-of-antibodies-to-recombinant-heat-shock-protein-70-in-immunohistochemical-diagnosis-of-mycobacterium-avium-subspecies-paratuberculosis-in-tissues-of-naturally-infected-cattle
#17
Julius Boniface Okuni, David Patrick Kateete, Moses Okee, Anna Nanteza, Moses Joloba, Lonzy Ojok
BACKGROUND: Detection of Mycobacterium avium subspecies paratuberculosis (MAP) infection is key to the control of Johne's disease. Immunohistochemistry is one of the methods of detection of MAP infection in tissues. However, unavailability of commercial antibodies that can detect the organism is a limiting factor for the use of immunohistochemistry. This study was aimed at developing an immunohistochemistry method to diagnose MAP in infected tissues using antibodies against MAP recombinant heat shock protein 70kd...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28249599/heat-shock-proteins-70-and-90-from-clonorchis-sinensis-induce-th1-response-and-stimulate-antibody-production
#18
Eun Joo Chung, Young-Il Jeong, Myoung-Ro Lee, Yu Jung Kim, Sang-Eun Lee, Shin-Hyeong Cho, Won-Ja Lee, Mi-Yeoun Park, Jung-Won Ju
BACKGROUND: Heat shock proteins (HSPs) are found in all prokaryotes and most compartments of eukaryotic cells. Members of the HSP family mediate immune responses to tissue damage or cellular stress. However, little is known about the immune response induced by the oriental liver fluke, Clonorchis sinensis, even though this organism is carcinogenic to humans. We address this issue in the present study in mouse bone marrow dendritic cells (mBMDCs), using recombinant HSP70 and 90 from C...
March 1, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28224252/heat-shock-protein-70-in-lung-and-kidney-of-specific-pathogen-free-chickens-is-a-receptor-associated-protein-that-interacts-with-the-binding-domain-of-the-spike-protein-of-infectious-bronchitis-virus
#19
ZhiKun Zhang, Xin Yang, PengWei Xu, Xuan Wu, Long Zhou, HongNing Wang
Avian infectious bronchitis virus (IBV) is a member of the family Coronaviridae. A binding domain that mediates the attachment of the virus to its receptor has been identified in the S1 protein of prototype IBV strain M41. In this study, we identified this binding domain in a different strain, as well as the cellular proteins that interact with it. First, we expressed the S1N proteins (residues 19-270) of M41 and another isolate, SCZJ3, and compared the binding capacities of recombinant S1N-M41 and S1N-SCZJ3 to host tissues...
June 2017: Archives of Virology
https://www.readbyqxmd.com/read/28201964/plasmodium-falciparum-heat-shock-protein-70-lacks-immune-modulatory-activity
#20
Ofentse Jacob Pooe, Gabriele Köllisch, Holger Heine, Addmore Shonhai
Heat shock protein 70 (Hsp70) family are conserved molecules that constitute a major part of the cell's protein folding machinery. The role of Hsp70s of parasitic origin in host cell immune modulation has remained contentious. This is largely due to the fact that several studies implicating Hsp70 in immune modulation rely on the use of recombinant protein derived from bacteria which is often fraught contamination. Thus, in the current study, we expressed recombinant Plasmodium falciparum Hsp70 (PfHsp70) using in three bacterial expression hosts: E...
February 14, 2017: Protein and Peptide Letters
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