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Recombinant hsp70

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https://www.readbyqxmd.com/read/28201964/plasmodium-falciparum-heat-shock-protein-70-lacks-immune-modulatory-activity
#1
AOfentse Jacob Pooe, Gabriele Köllisch, Holger Heine, Addmore Shonhai
Heat shock protein 70 (Hsp70) family are conserved molecules that constitute a major part of the cell's protein folding machinery. The role of Hsp70s of parasitic origin in host cell immune modulation has remained contentious. This is largely due to the fact that several studies implicating Hsp70 in immune modulation rely on the use of recombinant protein derived from bacteria which is often fraught contamination. Thus, in the current study, we expressed recombinant Plasmodium falciparum Hsp70 (PfHsp70) using in three bacterial expression hosts: E...
February 14, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28194450/assessment-of-the-adjuvant-activity-of-mesoporous-silica-nanoparticles-in-recombinant-mycoplasma-hyopneumoniae-antigen-vaccines
#2
Veridiana Gomes Virginio, Natalia Costantin Bandeira, Fernanda Munhoz Dos Anjos Leal, Marcelo Lancellotti, Arnaldo Zaha, Henrique Bunselmeyer Ferreira
The adjuvant potential of two mesoporous silica nanoparticles (MSNs), SBa-15 and SBa-16, was assessed in combination with a recombinant HSP70 surface polypeptide domain from Mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia (PEP). The recombinant antigen (HSP70212-600), previously shown as immunogenic in formulation with classic adjuvants, was used to immunize BALB/c mice in combination with SBa-15 or SBa-16 MSNs, and the effects obtained with these formulations were compared to those obtained with alum, the adjuvant traditionally used in anti-PEP bacterins...
January 2017: Heliyon
https://www.readbyqxmd.com/read/28152512/klotho-suppresses-the-inflammatory-responses-and-ameliorates-cardiac-dysfunction-in-aging-endotoxemic-mice
#3
Haipeng Hui, Yufeng Zhai, Lihua Ao, Joseph C Cleveland, Hongbin Liu, David A Fullerton, Xianzhong Meng
BACKGROUND: Aging augments endotoxemic cardiac dysfunction, but the mechanism remains unclear. Anti-aging protein Klotho has been found to modulate tissue inflammatory responses. We tested the hypothesis that a reduced Klotho level in aging heart plays a role in the augmented endotoxemic cardiac dysfunction. MATERIALS AND METHODS: Endotoxin (0.5 mg/kg, iv) was injected to adults (4-6 months) and aging (18-20 months) C57BL/6 mice. Recombinant Klotho (10 μg/kg, iv) was administered to a group of aging mice after endotoxin injection...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28106629/heat-shock-protein-70-overexpression-in-adipose-derived-stem-cells-enhances-fat-graft-survival
#4
Hao Feng, Lihong Qiu, Teng Zhang, Houyou Yu, Xianjie Ma, Yingjun Su, Hui Zheng, Yong Wang, Chenggang Yi
BACKGROUND: Autologous fat grafting is a prevalent technique used for soft-tissue augmentation; however, the poor survival rate of the grafted tissue remains a drawback of this method. Although adipose-derived stem cells (ASCs) are an attractive candidate for enhancing graft retention, the poor posttransplantation viability of these cells limits their application. Here we investigated whether overexpression of the antiapoptotic protein heat-shock protein 70 (Hsp70) could enhance ASCs' therapeutic potential for fat transplant survival...
January 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28064323/-heat-shock-protein-hsp70-reduces-the-secretion-of-tnf%C3%AE-by-neuroblastoma-cells-and-human-monocytes-induced-with-beta-amyloid-peptides
#5
M M Yurinskaya, V A Mit'kevich, M B Evgen'ev, A A Makarov, M G Vinokurov
The progress of neurodegeneration in Alzheimer's disease is closely associated with inflammatory processes in the brain tissues induced by beta-amyloid peptides (Aβ). In this paper, we showed that Aβ(1-42) and isoAβ(1-42) in human neuroblastoma cells SK-N-SH and promonocyte THP-1 activated the production of tumor necrosis factor (TNFα). Notably, isoAβ(1-42) had the strongest effect on the increase in the level of TNFα. The addition of recombinant heat-shock protein HSP70 reduces TNFα production induced by Aβ, which leads to a decrease in neuronal cell damage at the organism level...
November 2016: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28053106/grp78-is-an-important-host-factor-for-japanese-encephalitis-virus-entry-and-replication-in-mammalian-cells
#6
Minu Nain, Sriparna Mukherjee, Sonali Porey Karmakar, Adrienne W Paton, James C Paton, M Z Abdin, Anirban Basu, Manjula Kalia, Sudhanshu Vrati
: Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is the leading cause of viral encephalitis in South-East Asia with potential to become a global pathogen. Here we identify the Glucose regulated protein 78 (GRP78) as an important host protein for virus entry and replication. Using the plasma membrane fractions from mouse neuronal (Neuro2a) cells, mass spectroscopy analysis identified GRP78 as a protein interacting with recombinant JEV envelope protein domain III. GRP78 was found to express on the plasma membrane of Neuro2a, mouse primary neurons, and human epithelial Huh-7 cells...
January 4, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28026090/the-mesenchymal-precursor-cell-marker-antibody-stro-1-binds-to-cell-surface-heat-shock-cognate-70
#7
Stephen Fitter, Stan Gronthos, Soo Siang Ooi, Andrew C W Zannettino
Since its discovery more than 25 years ago, the STRO-1 antibody has played a fundamental role in defining the hierarchical nature of mesenchymal precursor cells (MPC) and their progeny. STRO-1 antibody binding remains a hallmark of immature pluripotent MPC. Despite the significance of STRO-1 in the MPC field, the identity of the antigen has remained elusive. Using a combination of two-dimensional gel electrophoresis, coupled with Western blotting and Tandem mass spectroscopy, we have identified the STRO-1 antigen as heat shock cognate 70 (HSC70;HSPA8)...
December 27, 2016: Stem Cells
https://www.readbyqxmd.com/read/28025138/functional-analysis-of-hspa1a-and-hspa8-in-parturition
#8
Junnan Geng, Huanan Li, Cong Huang, Jin Chai, Rong Zheng, Fenge Li, Siwen Jiang
Many factors are involved in parturition, such as apoptosis, inflammatory mediators, and hormones. Previous studies indicated that HSP70 directly or indirectly regulates apoptosis, inflammatory immune response and hormone stimulus. To gain new insights into molecular mechanism underlying HSP70 for regulating parturition, we overexpressed and knocked down two representative members of HSP70 (HSPA1A and HSPA8) through transfection of their recombinant plasmid and si-RNA separately in WISH (human amniotic epithelial) cells...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27977164/a-single-point-mutation-in-mitochondrial-hsp70-cochaperone-mge1-gains-thermal-stability-and-resistance
#9
Adinarayana Marada, Srinivasu Karri, Swati Singh, Praveen Kumar Allu, Yerranna Boggula, Thanuja Krishnamoorthy, Lalitha Guruprasad, Naresh Babu V Sepuri
Mge1, a yeast homologue of Escherichia coli GrpE, is an evolutionarily conserved homodimeric nucleotide exchange factor of mitochondrial Hsp70. Temperature-dependent reversible structural alteration from a dimeric to a monomeric form is critical for Mge1 to act as a thermosensor. However, very limited information about the structural component or amino acid residue(s) that contributes to thermal sensing of Mge1/GrpE is available. In this report, we have identified a single point mutation, His167 to Leu (H167L), within the hinge region of Mge1 that confers thermal resistance to yeast...
December 27, 2016: Biochemistry
https://www.readbyqxmd.com/read/27914463/study-of-immunomodulatory-effects-of-extracellular-hsp70-in-a-mouse-model-of-allergic-airway-inflammation
#10
M A Shevchenko, N I Troyanova, E A Servuli, E L Bolkhovitina, A S Fedorina, A M Sapozhnikov
Immunostimulatory properties of extracellular heat shock proteins 70 kDa (HSP70) became interesting for investigators a long time ago. However, in recent years a series of works showing a significant relation of the immunostimulating effects of recombinant HSP70 to contamination of the protein samples with bacterial endotoxins (lipopolysaccharide, LPS) has been published. The authors showed that intensive elimination of LPS from the protein samples resulted in inversion of immunostimulating effects of HSP70 to immunosuppressive activity of the protein...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27847173/enhanced-protective-efficacy-against-tuberculosis-provided-by-a-recombinant-urease-deficient-bcg-expressing-heat-shock-protein-70-major-membrane-protein-ii-having-pest-sequence
#11
Yumiko Tsukamoto, Yumi Maeda, Toshiki Tamura, Tetsu Mukai, Satoshi Mitarai, Saburo Yamamoto, Masahiko Makino
Enhancement of the T cell-stimulating ability of Mycobacterium bovis BCG (BCG) is necessary to develop an effective tuberculosis vaccine. For this purpose, we introduced the PEST-HSP70-major membrane protein-II (MMPII)-PEST fusion gene into ureC-gene depleted recombinant (r) BCG to produce BCG-PEST. The PEST sequence is involved in the proteasomal processing of antigens. BCG-PEST secreted the PEST-HSP70-MMPII-PEST fusion protein and more efficiently activated human monocyte-derived dendritic cells (DCs) in terms of phenotypic changes and cytokine productions than an empty-vector-introduced BCG or HSP70-MMPII gene-introduced ureC gene-depleted BCG (BCG-DHTM)...
December 7, 2016: Vaccine
https://www.readbyqxmd.com/read/27828760/ginseng-stem-and-leaf-saponins-gsls-enhanced-protective-immune-responses-induced-by-toxoplasma-gondii-heat-shocked-protein-70-hsp70-against-toxoplasmosis-in-mice
#12
Xunhui Zhuo, Hongchao Sun, Suhua Wang, Xiaolu Guo, Haojie Ding, Yi Yang, Ying Shan, Aifang Du
Toxoplasma gondii is an obligate intracellular protozoan parasite and is able to infect birds and mammals including humans. In order to find effective antigen-adjuvant combinations that can boost the immunogenicity and protection of antigen vaccines against toxoplasmosis, we examined the protective efficacy in mice immunized with recombinant protein HSP70 when co-administered with ginseng stem-and-leaf saponins (GSLS) isolated from Panax ginseng. All immunized mice produced significantly high levels of specific antibodies against rTgHSP70 and splenocytes from mice presented strong proliferative immune responses...
November 9, 2016: Journal of Parasitology
https://www.readbyqxmd.com/read/27822032/co-delivery-of-doxorubicin-and-recombinant-plasmid-phsp70-plk1-shrna-by-bacterial-magnetosomes-for-osteosarcoma-therapy
#13
Li Cheng, Youqun Ke, Shuisheng Yu, Juehua Jing
To explore a novel combination of chemotherapy, gene therapy, and thermotherapy for osteosarcoma, a targeted heat-sensitive co-delivery system based on bacterial magnetosomes (BMs) was developed. The optimal culture conditions of magnetotactic bacteria (MTB) AMB-1 and characterization of BMs were achieved. A recombinant eukaryotic plasmid heat shock protein 70-polo-like kinase 1-short hairpin RNA (pHSP70-Plk1-shRNA) under transcriptional control of a thermosensitive promoter (human HSP70 promoter) was constructed for gene therapy...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27807229/evidence-that-hsc70-is-associated-with-cucumber-necrosis-virus-particles-and-plays-a-role-in-particle-disassembly
#14
Syed Benazir Alam, D'Ann Rochon
: Uncoating of a virus particle to expose its nucleic acid is a critical aspect of the viral multiplication cycle, as it is essential for the establishment of infection. In the present study, we investigated the role of plant HSP70 homologs in the uncoating process of Cucumber necrosis virus (CNV), a nonenveloped positive-sense single-stranded RNA [(+)ssRNA] virus having a T=3 icosahedral capsid. We have found through Western blot analysis and mass spectrometry that the HSP70 homolog Hsc70-2 copurifies with CNV particles...
January 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27783274/encapsulated-hsp70-decreases-endotoxin-induced-production-of-ros-and-tnf%C3%AE-in-human-phagocytes
#15
Yurinskaya M M, Kochetkova O Yu, Shabarchina L I, Antonova O Yu, Suslikov A V, Evgen'ev M B, Vinokurov M G
Human heat shock protein Hsp70 was experimentally inserted into polyelectrolyte microcapsules. Encapsulated recombinant Hsp70 was studied in terms of its effects on neutrophil apoptosis, the production of reactive oxygen species, and the secretion of tumor necrosis factor alpha by promonocytic THP-1 cells. It was found that encapsulated Hsp70 effectively inhibits neutrophil apoptosis, unlike free exogenous protein used in solution. In THP-1 cells, encapsulated and free Hsp70 reduced LPS-induced tumor necrosis factor alpha production with a similar efficiency...
January 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/27753175/prokaryotic-expression-of-chicken-interferon-%C3%AE-fusion-protein-and-its-effect-on-expression-of-poultry-heat-shock-protein-70-under-heat-stress
#16
Jinhua Sun, Yinglin Chen, Feiyue Qin, Xueting Guan, Wei Xu, Liangmei Xu
Interferons have attracted considerable attention due to their vital roles in the host immune response and low induction of antibiotic resistance. In this study, total RNA was extracted from spleen cells of chicken embryos inoculated with Newcastle disease vaccine, and the full-length chicken interferon-γ (ChIFN-γ) gene was amplified by RT-PCR. The full complementary DNA sequence of the ChIFN-γ gene was 495 bp long and was cloned into the prokaryotic expression vector pProEX™HTb . The plasmid was transformed into Escherichia coli DH5α and the expression of ChIFN-γ was induced by isopropyl β-D-1-thiogalactopyranoside...
October 17, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27713135/recombinant-heat-shock-protein-70-functional-peptide-and-alpha-fetoprotein-epitope-peptide-vaccine-elicits-specific-anti-tumor-immunity
#17
Xiao-Ping Wang, Qiao-Xia Wang, Huan-Ping Lin, Bing Xu, Qian Zhao, Kun Chen
Alpha-fetoprotein (AFP) is a marker of hepatocellular carcinoma (HCC) and serves as a target for immunotherapy. However, current treatments targeting AFP are not reproducible and do not provide complete protection against cancer. This issue may be solved by developing novel therapeutic vaccines with enhanced immunogenicity that could effectively target AFP-expressing tumors. In this study, we report construction of a therapeutic peptide vaccine by linking heat shock protein 70 (HSP70) functional peptide to the AFP epitope to obtain HSP70-P/AFP-P...
1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27605553/heat-shock-protein-based-therapy-as-a-potential-candidate-for-treating-the-sphingolipidoses
#18
Thomas Kirkegaard, James Gray, David A Priestman, Kerri-Lee Wallom, Jennifer Atkins, Ole Dines Olsen, Alexander Klein, Svetlana Drndarski, Nikolaj H T Petersen, Linda Ingemann, David A Smith, Lauren Morris, Claus Bornæs, Signe Humle Jørgensen, Ian Williams, Anders Hinsby, Christoph Arenz, David Begley, Marja Jäättelä, Frances M Platt
Lysosomal storage diseases (LSDs) often manifest with severe systemic and central nervous system (CNS) symptoms. The existing treatment options are limited and have no or only modest efficacy against neurological manifestations of disease. We demonstrate that recombinant human heat shock protein 70 (HSP70) improves the binding of several sphingolipid-degrading enzymes to their essential cofactor bis(monoacyl)glycerophosphate in vitro. HSP70 treatment reversed lysosomal pathology in primary fibroblasts from 14 patients with eight different LSDs...
September 7, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27537186/elongation-factor-tu-and-heat-shock-protein-70-are-membrane-associated-proteins-from-mycoplasma-ovipneumoniae-capable-of-inducing-strong-immune-response-in-mice
#19
Fei Jiang, Jinyan He, Nalu Navarro-Alvarez, Jian Xu, Xia Li, Peng Li, Wenxue Wu
Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu) and heat shock protein 70 (HSP 70) are membrane-associated proteins on M. ovipneumonaiea. We have evaluated the humoral and cellular immune responses in vivo by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70...
2016: PloS One
https://www.readbyqxmd.com/read/27458447/a-recombinant-trivalent-fusion-protein-f1-lcrv-hsp70-ii-augments-humoral-and-cellular-immune-responses-and-imparts-full-protection-against-yersinia-pestis
#20
Shailendra K Verma, Lalit Batra, Urmil Tuteja
Plague is one of the most dangerous infections in humans caused by Yersinia pestis, a Gram-negative bacterium. Despite of an overwhelming research success, no ideal vaccine against plague is available yet. It is well established that F1/LcrV based vaccine requires a strong cellular immune response for complete protection against plague. In our earlier study, we demonstrated that HSP70(II) of Mycobacterium tuberculosis modulates the humoral and cellular immunity of F1/LcrV vaccine candidates individually as well as in combinations in a mouse model...
2016: Frontiers in Microbiology
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