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bladder tissue engineering

Jakub Smolar, Souzan Salemi, Maya Horst, Tullio Sulser, Daniel Eberli
Conditions impairing bladder function in children and adults, such as myelomeningocele, posterior urethral valves, bladder exstrophy or spinal cord injury, often need urinary diversion or augmentation cystoplasty as when untreated they may cause severe bladder dysfunction and kidney failure. Currently, the gold standard therapy of end-stage bladder disease refractory to conservative management is enterocystoplasty, a surgical enlargement of the bladder with intestinal tissue. Despite providing functional improvement, enterocystoplasty is associated with significant long-term complications, such as recurrent urinary tract infections, metabolic abnormalities, stone formation, and malignancies...
September 2016: Transfusion Medicine and Hemotherapy
Massimo Alfano, Manuela Nebuloni, Raffaele Allevi, Pietro Zerbi, Erika Longhi, Roberta Lucianò, Irene Locatelli, Angela Pecoraro, Marco Indrieri, Chantal Speziali, Claudio Doglioni, Paolo Milani, Francesco Montorsi, Andrea Salonia
In the fields of biomaterials and tissue engineering simulating the native microenvironment is of utmost importance. As a major component of the microenvironment, the extracellular matrix (ECM) contributes to tissue homeostasis, whereas modifications of native features are associated with pathological conditions. Furthermore, three-dimensional (3D) geometry is an important feature of synthetic scaffolds favoring cell stemness, maintenance and differentiation. We analyzed the 3D structure, geometrical measurements and anisotropy of the ECM isolated from (i) human bladder mucosa (basal lamina and lamina propria) and muscularis propria; and, (ii) bladder carcinoma (BC)...
October 25, 2016: Scientific Reports
Gisela Reinfeldt Engberg, Clara Ibel Chamorro, Agneta Nordenskjöld, Magdalena Fossum
In order to develop autologous tissue engineering of the whole wall in the urinary excretory system, we studied the regenerative capacity of the muscular bladder wall. Smooth muscle cell expansion on minced detrusor muscle in vitro and in vivo with or without urothelial tissue was studied. Porcine minced detrusor muscle and urothelium were cultured in vitro under standard culture conditions for evaluation of the explant technique and in collagen for tissue sectioning and histology. Autografts of minced detrusor muscle with or without minced urothelium were expanded on 3D cylinder moulds by grafting into the subcutaneous fat of the pig abdominal wall...
2016: BioMed Research International
Natalia M Yudintceva, Yulia A Nashchekina, Miralda I Blinova, Nadezhda V Orlova, Alexandr N Muraviov, Tatiana I Vinogradova, Magomed G Sheykhov, Elena Y Shapkova, Dmitriy V Emeljannikov, Petr K Yablonskii, Igor A Samusenko, Anastasiya L Mikhrina, Artem V Pakhomov, Maxim A Shevtsov
In the present study, a poly-l-lactide/silk fibroin (PL-SF) bilayer scaffold seeded with allogenic bone marrow stromal cells (BMSCs) was investigated as a potential approach for bladder tissue engineering in a model of partial bladder wall cystectomy in rabbits. The inner porous layer of the scaffold produced from silk fibroin was designed to promote cell proliferation and the outer layer produced from poly-l-lactic acid to serve as a waterproof barrier. To compare the feasibility and efficacy of BMSC application in the reconstruction of bladder defects, 12 adult male rabbits were divided into experimental and control groups (six animals each) that received a scaffold seeded with BMSCs or an acellular one, respectively...
2016: International Journal of Nanomedicine
Jia Li, Wenguang Wang, Hengqing An, Feng Wang, Mulati Rexiati, Yujie Wang
BACKGROUND: The aim of this work was to create a xenogeneic cell scaffold complex with rabbit bladder acellular matrix and rat hair follicle stem cells, to study the feasibility of construct tissue engineer bladder through biocompatibility of hair follicle stem cells and heterogeneous bladder acellular matrix. MATERIAL AND METHODS: New Zealand rabbit bladder acellular matrix was prepared. Scanning electron microscope and Masson staining were used to analyse the acellular material...
2016: SpringerPlus
Jaehong Key, Deepika Dhawan, Christy L Cooper, Deborah W Knapp, Kwangmeyung Kim, Ick Chan Kwon, Kuiwon Choi, Kinam Park, Paolo Decuzzi, James F Leary
While current imaging modalities, such as magnetic resonance imaging (MRI), computed tomography, and positron emission tomography, play an important role in detecting tumors in the body, no single-modality imaging possesses all the functions needed for a complete diagnostic imaging, such as spatial resolution, signal sensitivity, and tissue penetration depth. For this reason, multimodal imaging strategies have become promising tools for advanced biomedical research and cancer diagnostics and therapeutics. In designing multimodal nanoparticles, the physicochemical properties of the nanoparticles should be engineered so that they successfully accumulate at the tumor site and minimize nonspecific uptake by other organs...
2016: International Journal of Nanomedicine
Bülent Çetinel, Ervin Kocjancic, Çetin Demirdağ
The aim of this review is to update the indications, contraindications, technique, complications, and the tissue engineering approaches of augmentation cystoplasty (AC) in patients with neurogenic bladder. PubMed/MEDLINE was searched for the keywords "augmentation cystoplasty," "neurogenic bladder," and "bladder augmentation." Additional relevant literature was determined by examining the reference lists of articles identified through the search. The update review of of the indications, contraindications, technique, outcome, complications, and tissue engineering approaches of AC in patients with neurogenic bladder is presented...
September 2016: Investigative and Clinical Urology
Marta Pokrywczynska, Daria Balcerczyk, Arkadiusz Jundzill, Maciej Gagat, Monika Czapiewska, Tomasz Kloskowski, Maciej Nowacki, Agata M Gastecka, Magdalena Bodnar, Alina Grzanka, Andrzej Marszalek, Tomasz Drewa
BACKGROUND: A key requirements for therapy utilizing the tissue engineering methodologies is use of techniques which have the capability to yield a high number of cells, from small tissue biopsy in a relatively short time. Up to date there was no optimal methods of isolation and expansion of urinary bladder smooth muscle cells (UB-SMCs). The aim of this study was to compare isolation and expansion techniques of UB-SMCs to select the most repeatable and efficient one. METHOD: Five protocols of porcine UB- SMCs isolation including enzymatic and explant techniques and three expansion techniques were compared...
2016: Biological Procedures Online
Nayereh Vahedi Mazdabadi, Elahe Karimpour Razkenari, Mina Saeedi, Tahmineh Akbarzadeh, Mohammad Toushih
BACKGROUND: It is evident that infant's umbilical cord is essential for its survival and growth. In the past, it was not considered as a necessary tissue. However, nowadays, it has attracted lots of attention and its relation to different diseases has been demonstrated. In Iranian traditional medicine, caring for newborn's umbilical cord has been frequently recommended and it has been associated with different diseases. METHODS: Herein, Iranian traditional medicine resources such as "Qanun-fil-Tibb", "Exir-e-Azam", and "Tib-e-Akbari" were studied...
May 2016: Iranian Journal of Medical Sciences
Hiroshi Fukuhara, Yasushi Ino, Tomoki Todo
Oncolytic virus therapy is perhaps the next major breakthrough in cancer treatment following the success in immunotherapy using immune checkpoint inhibitors. Oncolytic viruses are defined as genetically engineered or naturally occurring viruses that selectively replicate in and kill cancer cells without harming the normal tissues. T-Vec (talimogene laherparepvec), a second-generation oncolytic herpes simplex virus type 1 (HSV-1) armed with GM-CSF, was recently approved as the first oncolytic virus drug in the USA and Europe...
August 3, 2016: Cancer Science
Henk R Hoogenkamp, Michiel W Pot, Theo G Hafmans, Dorien M Tiemessen, Yi Sun, Egbert Oosterwijk, Wout F Feitz, Willeke F Daamen, Toin H van Kuppevelt
UNLABELLED: The field of regenerative medicine has developed promising techniques to improve current neobladder strategies used for radical cystectomies or congenital anomalies. Scaffolds made from molecularly defined biomaterials are instrumental in the regeneration of tissues, but are generally confined to small flat patches and do not comprise the whole organ. We have developed a simple, one-step casting method to produce a seamless large hollow collagen-based scaffold, mimicking the shape of the whole bladder, and with integrated anastomotic sites for ureters and urethra...
October 1, 2016: Acta Biomaterialia
R G Frykberg, S M Cazzell, J Arroyo-Rivera, A Tallis, A M Reyzelman, F Saba, L Warren, B C Stouch, T W Gilbert
OBJECTIVE: The objective of this prospective, multicentre clinical study is to assess the application of MatriStem MicroMatrix (MSMM) and MatriStem Wound Matrix (MSWM) (porcine urinary bladder derived extracellular matrix) compared with Dermagraft (DG) (human fibroblast-derived dermal substitute) for the management of non-healing diabetic foot ulcers (DFUs). METHOD: A randomised, multicentre study was conducted at thirteen centers throughout the US. It was designed to evaluate the incidence of ulcer closure, rate of ulcer healing, wound characteristics, patient quality of life, cost-effectiveness, and recurrence...
July 2016: Journal of Wound Care
Stéphane Chabaud, Melissa Simard, Isabelle Gendreau, Roxane Pouliot, Stéphane Bolduc
Despite the emergence of serum-free media for cell culture, the use of serum to supplement the culture media is still essential in order to produce engineered urologic tissues using the self-assembly approach, not only for the stromal compartment but also for the uroepithelium. Effects of sera on thickness of these two compartments were measured and quality of the epithelial differentiation was evaluated. For bladder mucosa substitute reconstruction, the use of postnatal sera failed to produce an adequate uroepithelium whereas the fetal sera supplementation did...
2016: Scientifica
Stuart J Emmerson, Caroline E Gargett
Pelvic organ prolapse (POP) occurs when the pelvic organs (bladder, bowel or uterus) herniate into the vagina, causing incontinence, voiding, bowel and sexual dysfunction, negatively impacting upon a woman's quality of life. POP affects 25% of all women and results from childbirth injury. For 19% of all women, surgical reconstructive surgery is required for treatment, often augmented with surgical mesh. The surgical treatment fails in up to 30% of cases or results in adverse effects, such as pain and mesh erosion into the bladder, bowel or vagina...
May 26, 2016: World Journal of Stem Cells
Xianglin Hou, Chunying Shi, Wei Chen, Bing Chen, Weisheng Jia, Yu Guo, Chao Ma, Gang Ye, Jiuhong Kang, Jianwu Dai
Tissue engineering brings new hope for the reconstruction of injured bladders. The aim of the present study was to evaluate human adipose-derived mesenchymal stem cells (hADSCs) combined with a bladder acellular matrix (BAM) for bladder regeneration. A BAM or BAM loaded with hADSCs (BAM/hADSCs) was used to repair partial cystectomy of the bladder in a canine model. 6 months after implantation, calculi and urine leakage were not found in either the BAM or BAM/hADSCs group by cystography. And compared to the BAM group, a significant increase of maximum bladder volume and bladder compliance was observed in the BAM/hADSCs group by urodynamics evaluation...
2016: Biomedical Materials
C Alberti
To prevent problematic outcomes of bowel-based bladder reconstructive surgery, such as prosthetic tumors and systemic metabolic complications, research works, to either regenerate and strengthen failing organ or build organ replacement biosubstitute, have been turned, from 90s of the last century, to both regenerative medicine and tissue engineering.Various types of acellular matrices, naturally-derived materials, synthetic polymers have been used for either "unseeded" (cell free) or autologous "cell seeded" tissue engineering scaffolds...
January 2016: Il Giornale di Chirurgia
Marta Pokrywczynska, Monika Czapiewska, Arkadiusz Jundzill, Magdalena Bodnar, Daria Balcerczyk, Tomasz Kloskowski, Maciej Nowacki, Andrzej Marszalek, Tomasz Drewa
Many experimental approaches have been conducted in order to isolate urothelial cells from bladder tissue biopsies, but each method described has utilized different protocols and sources of bladder tissue. In this study, we compared the different methods of urothelial cell isolation available in literature together with standardized methods in order to obtain more unified results. Five methods for primary porcine urothelial culture establishment were compared: tissue explants and four enzymatic methods utilizing collagenase II, dispase II, combination of dispase II and trypsin, and trypsin alone...
July 2016: Cell Biology International
Mohammad Ali Derakhshan, Gholamreza Pourmand, Jafar Ai, Hossein Ghanbari, Rassoul Dinarvand, Mohammad Naji, Reza Faridi-Majidi
PURPOSE: Urinary bladder may encounter several pathologic conditions that could lead to loss of its function. Tissue engineering using electrospun PLLA scaffolds is a promising approach to reconstructing or replacing the problematic bladder. METHODS: PLLA nanofibrous scaffolds were prepared utilizing single-nozzle electrospinning. The morphology and distribution of fiber diameters were investigated by scanning electron microscopy (SEM). Human bladder smooth muscle cells (hBSMCs) were isolated from biopsies and characterized by immunocytochemistry (ICC)...
July 2016: International Urology and Nephrology
Areg Zingiryan, Nicholas H Farina, Kristiaan H Finstad, Janet L Stein, Jane B Lian, Gary S Stein
Genetically engineered mouse models of prostate cancer allow for study of disease progression from localized tumor formation through distal metastasis. The anatomy of the mouse prostate differs dramatically from the human prostate, being composed of four lobe pairs (anterior, dorsal, lateral, and ventral), making the identification and dissection technically challenging. Although the entire murine prostate and surrounding tissue, including urethra, bladder, seminal vesicles, and associated adipose tissue, can be quickly dissected for en bloc analysis, it is necessary to isolate individual prostate lobes for gene expression studies elucidating the molecular mechanisms of prostate cancer...
January 2017: Journal of Cellular Physiology
Günther Bonifert, Lisa Folkes, Christoph Gmeiner, Gabi Dachs, Oliver Spadiut
Cancer is a major cause of death. Common chemo- and radiation-therapies damage healthy tissue and cause painful side effects. The enzyme horseradish peroxidase (HRP) has been shown to activate the plant hormone indole-3-acetic acid (IAA) to a powerful anticancer agent in in vitro studies, but gene directed enzyme prodrug therapy (GDEPT) studies showed ambivalent results. Thus, HRP/IAA in antibody directed enzyme prodrug therapy (ADEPT) was investigated as an alternative. However, this approach has not been intensively studied, since the enzyme preparation from plant describes an undefined mixture of isoenzymes with a heterogenic glycosylation pattern incompatible with the human system...
June 2016: Cancer Medicine
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