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https://www.readbyqxmd.com/read/28808033/multiplex-quantitative-cellular-analysis-in-large-tissue-volumes-with-clearing-enhanced-3d-microscopy-ce3d
#1
Weizhe Li, Ronald N Germain, Michael Y Gerner
Organ homeostasis, cellular differentiation, signal relay, and in situ function all depend on the spatial organization of cells in complex tissues. For this reason, comprehensive, high-resolution mapping of cell positioning, phenotypic identity, and functional state in the context of macroscale tissue structure is critical to a deeper understanding of diverse biological processes. Here we report an easy to use method, clearing-enhanced 3D (Ce3D), which generates excellent tissue transparency for most organs, preserves cellular morphology and protein fluorescence, and is robustly compatible with antibody-based immunolabeling...
August 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28801951/modeling-ion-recombination-in-liquid-ionization-chambers-improvement-and-analysis-of-the-two-dose-rate-method
#2
Jonas Andersson, Heikki Tölli
PURPOSE: The use of liquid ionization chambers can provide useful information to endeavors with radiation dosimetry for highly modulated beams. Liquid ionization chambers may be particularly suitable for computed tomography applications where conventional ionization chambers do not present a high enough sensitivity for the spatial resolution required to characterize common X-ray beams. Due to the sensitivity, which leads to high charge densities, liquid ionization chambers can suffer from large recombination losses leading to degradation in signal to dose rate linearity...
August 12, 2017: Medical Physics
https://www.readbyqxmd.com/read/28737172/selective-production-of-arenes-via-direct-lignin-upgrading-over-a-niobium-based-catalyst
#3
Yi Shao, Qineng Xia, Lin Dong, Xiaohui Liu, Xue Han, Stewart F Parker, Yongqiang Cheng, Luke L Daemen, Anibal J Ramirez-Cuesta, Sihai Yang, Yanqin Wang
Lignin is the only large-volume renewable source of aromatic chemicals. Efficient depolymerization and deoxygenation of lignin while retaining the aromatic functionality are attractive but extremely challenging. Here we report the selective production of arenes via direct hydrodeoxygenation of organosolv lignin over a porous Ru/Nb2O5 catalyst that enabled the complete removal of the oxygen content from lignin. The conversion of birch lignin to monomer C7-C9 hydrocarbons is nearly quantitative based on its monomer content, with a total mass yield of 35...
July 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28734758/atypical-interference-control-in-children-with-ad-hd-with-elevated-theta-beta-ratio
#4
Da-Wei Zhang, Steven Roodenrys, Hui Li, Robert J Barry, Adam R Clarke, Zhanliang Wu, Qihua Zhao, Yan Song, Lu Liu, Qiujin Qian, Yufeng Wang, Stuart J Johnstone, Li Sun
The theta/beta ratio (TBR) is a major area of interest within electroencephalogram (EEG) research in AD/HD. While researchers suggest a prognostic role for TBR in AD/HD, its relationship to behavior remains uncertain. Recent evidence suggests that elevated TBR in AD/HD may be related to atypical inhibition, particularly at an attentional level. This study aimed to examine the performance on three inhibitory tasks of children with AD/HD. Fifty-eight children with AD/HD participated, divided into an elevated TBR (ET) group and a control group (CT)...
July 20, 2017: Biological Psychology
https://www.readbyqxmd.com/read/28712467/children-disassociate-from-antisocial-in-group-members
#5
Matti Wilks, Mark Nielsen
Extensive research has demonstrated that children show a robust in-group bias and, concurrently, are highly attuned to the prosocial and antisocial behavior of others. The limited research investigating the capacity for antisocial behavior to attenuate children's in-group bias has, however, returned mixed findings. Moreover, no research has examined how this might interact with perceived group permeability. Thus, the current study aimed to provide a more complete understanding of the relationship between in-group bias and antisocial behavior, how this interacts with perceptions of out-group behavior, and how group context (permeability) influences these responses...
July 13, 2017: Journal of Experimental Child Psychology
https://www.readbyqxmd.com/read/28710270/stabilization-of-multimeric-proteins-via-inter-subunits-cyclization
#6
Lu Zhu, Shuwen Wang, Wenya Tian, Yeqi Zhang, Yang Song, Jun Zhang, Bo Mu, Chao Peng, Zixin Deng, Hongmin Ma, Xudong Qu
Proteins with high catalytic efficiency and selectivity under mild conditions have long been appreciated by industrial and medicinal fields. These proteins, which are commonly multimeric, often possess low stability impeding wider application. Currently, strategies to improve the stability of multimeric proteins concentrate on enhancing the interaction at internal-interface of the subunits. In this report, we confirmed that the largely underestimated subunit terminal ends are as significant as the internal-interface for protein stability...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28704806/a-molecular-rotor-based-ratiometric-sensor-for-basic-amino-acids
#7
Aafrin M Pettiwala, Prabhat K Singh
The inevitable importance of basic amino acids, arginine and lysine, in human health and metabolism demands construction of efficient sensor systems for them. However, there are only limited reports on the 'ratiometric' detection of basic amino acids which is further restricted by the use of chemically complex sensor molecules, which impedes their prospect for practical applications. Herein, we report a ratiometric sensor system build on simple mechanism of disassociation of novel emissive Thioflavin-T H-aggregates from heparin surface, when subjected to interaction with basic amino acids...
June 29, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28702389/correlation-between-the-elbow-flexion-and-the-hand-and-wrist-flexion-after-neurotization-of-the-fascicles-of-the-ulnar-nerve-to-the-motor-branch-to-the-biceps
#8
Ricardo Boso Escudero, Marcelo Rosa de Rezende, Erick Yoshio Wataya, Fernando Vicente de Pontes, Álvaro Baik Cho, Marina Justi Pisani
OBJECTIVE: Gain in elbow flexion in patients with brachial plexus injury is extremely important. The transfer of a fascicle from the ulnar nerve to the motor branch of the musculocutaneous nerve (Oberlin surgery) is a treatment option. However, in some patients, gain in elbow flexion is associated with wrist and finger flexion. This study aimed to assess the frequency of this association and the functional behavior of the limb. METHODS: Case-control study of 18 patients who underwent the Oberlin surgery...
May 2017: Revista Brasileira de Ortopedia
https://www.readbyqxmd.com/read/28670836/epithelial-cadherin-disassociates-from-the-lateral-plasma-membrane-of-uterine-epithelial-cells-throughout-pregnancy-in-a-marsupial
#9
Jessica S Dudley, Christopher R Murphy, Michael B Thompson, Bronwyn M McAllan
The uterine luminal epithelium is the first site of contact between fetal and maternal tissues during therian pregnancy and must undergo specialised changes for implantation of the blastocyst to be successful. These changes, collectively termed the plasma membrane transformation (PMT), allow the blastocyst to attach to the uterine epithelium preceding the formation of a placenta. There are similarities in the morphological and molecular changes occurring in live-bearing eutherian species during the PMT studied so far...
July 3, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28665971/biophysical-induction-of-cell-release-for-minimally-manipulative-cell-enrichment-strategies
#10
Pascal Joly, Thomas Schaus, Andrea Sass, Anke Dienelt, Alexander S Cheung, Georg N Duda, David J Mooney
The use of autologous cells harvested and subsequently transplanted in an intraoperative environment constitutes a new approach to promote regeneration. Usually cells are isolated by selection methods such as fluorescence- or magnetic- activated cell sorting with residual binding of the antibodies or beads. Thus, cell-based therapies would benefit from the development of new devices for cell isolation that minimally manipulate the target cell population. In the clinic, 5 to 10 percent of fractures do not heal properly and CD31+ cells have been identified as promising candidates to support bone regeneration...
2017: PloS One
https://www.readbyqxmd.com/read/28662478/disassociation-of-cognitive-and-affective-aspects-of-theory-of-mind-in-obsessive-compulsive-disorder
#11
Wanting Liu, Jie Fan, Jun Gan, Hui Lei, Chaoyang Niu, Raymond C K Chan, Xiongzhao Zhu
Impairment in social functioning has been widely described in obsessive-compulsive disorder (OCD). However, several aspects of social cognition, such as theory of mind (ToM), have not been substantially investigated in this context. This study examined cognitive and affective ToM in 40 OCD patients and 38 age-, sex-, and education-matched healthy controls (HCs) with the computerized Yoni task and a battery of neurocognitive tests. OCD symptom severity was assessed with the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS)...
September 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28643347/off-resonance-based-assessment-of-metallic-wear-debris-near-total-hip-arthroplasty
#12
Kevin M Koch, Matthew F Koff, Thomas W Bauer, Parina H Shah, Andrew S Nencka, S Sivaram Kaushik, Hollis G Potter
PURPOSE: The presence of metallic debris near total hip arthroplasty can have a significant impact on longitudinal patient management. Methods for magnetic resonance imaging-based quantification of metallic debris near painful total hip replacements are described and applied to cohorts of symptomatic and control subject cases. METHODS: A combination of metal artifact reduction, off-resonance mapping, off-resonance background removal, and spatial clustering methods are utilized to quantify off-resonance signatures in cases of suspected metallosis...
June 22, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28629211/effect-of-methoxsalen-on-ca%C3%A2-%C3%A2-%C2%BA-homeostasis-and-viability-in-human-osteosarcoma-cells
#13
Yi-Chau Lu, Chiang-Ting Chou, Wei-Zhe Liang, Chun-Chi Kuo, Shu-Shong Hsu, Jue-Long Wang, Chung-Ren Jan
Methoxsalen is a natural compound found in many seed plants. The effect of methoxsalen on Ca²⁺- related physiology in human osteosarcoma is unclear. This study investigated the effect of methoxsalen on cytosolic free Ca²⁺ concentrations ([Ca²⁺]i) in MG63 human osteosarcoma cells. Methoxsalen induced [Ca²⁺]i rises concentration-dependently. Methoxsalen-induced Ca²⁺ entry was confirmed by Mn²⁺-induced quench of fura-2 fluorescence. This Ca²⁺ entry was suppressed by nifedipine, econazole, and SKF96365...
June 30, 2017: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/28599311/c-terminal-kinesin-motor-kifc1-participates-in-facilitating-proper-cell-division-of-human-seminoma
#14
Yu-Xi Xiao, Hao-Qing Shen, Zhen-Yu She, Li Sheng, Qian-Qian Chen, Yu-Lan Chu, Fu-Qing Tan, Wan-Xi Yang
C-terminus kinesin motor KIFC1 is known for centrosome clustering in cancer cells with supernumerary centrosomes. KIFC1 crosslinks and glides on microtubules (MT) to assist normal bipolar spindle formation to avoid multi-polar cell division, which might be fatal. Testis cancer is the most common human cancer among young men. However, the gene expression profiles of testis cancer is still not complete and the expression of the C-terminus kinesin motor KIFC1 in testis cancer has not yet been examined. We found that KIFC1 is enriched in seminoma tissues in both mRNA level and protein level, and is specifically enriched in the cells that divide actively...
May 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28598606/4s-hydroxylation-of-insulin-at-prob28-accelerates-hexamer-dissociation-and-delays-fibrillation
#15
Seth A Lieblich, Katharine Y Fang, Jackson K B Cahn, Jeffrey Rawson, Jeanne LeBon, H Teresa Ku, David A Tirrell
Daily injections of insulin provide lifesaving benefits to millions of diabetics. But currently available prandial insulins are suboptimal: The onset of action is delayed by slow dissociation of the insulin hexamer in the subcutaneous space, and insulin forms amyloid fibrils upon storage in solution. Here we show, through the use of noncanonical amino acid mutagenesis, that replacement of the proline residue at position 28 of the insulin B-chain (ProB28) by (4S)-hydroxyproline (Hzp) yields an active form of insulin that dissociates more rapidly, and fibrillates more slowly, than the wild-type protein...
June 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28594110/novel-application-of-fluorescence-coupled-capillary-electrophoresis-to-resolve-the-interaction-between-the-g-quadruplex-aptamer-and-thrombin
#16
Jianhao Wang, Yaqin Gu, Li Liu, Cheli Wang, Jianpeng Wang, Shumin Ding, Jinping Li, Lin Qiu, Pengju Jiang
The dynamic binding status between the thrombin and its G-quadruplex aptamers and the stability of its interaction partners were probed using our previously established fluorescence-coupled capillary electrophoresis method. A 29-nucleic acid thrombin binding aptamer was chosen as a model to study its binding affinity with the thrombin ligand. First, the effects of the cations on the formation of G-quadruplex from unstructured 29-nucleic acid thrombin binding aptamer were examined. Second, the rapid binding kinetics between the thrombin and 6-carboxyfluorescein labeled G-quadruplex aptamer was measured...
June 8, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28592812/activity-independent-targeting-of-mtor-to-lysosomes-in-primary-osteoclasts
#17
Andrew Wang, Luciene R Carraro-Lacroix, Celeste Owen, Bowen Gao, Paul N Corey, Pascal Tyrrell, John H Brumell, Irina Voronov
Mammalian target of rapamycin (mTOR) is activated by numerous stimuli, including amino acids and growth factors. This kinase is part of the mTOR complex 1 (mTORC1) which regulates cell proliferation, differentiation, and autophagy. Active mTORC1 is located on lysosomes and has been reported to disassociate from the lysosomal surface in the absence of amino acids. Furthermore, mTORC1 activity has been linked to the vacuolar H(+)-ATPases (V-ATPases), the proton pumps responsible for lysosomal acidification; however, the exact role of the V-ATPases in mTORC1 signaling is not known...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28572446/histone-deacetylase-1-activates-pu-1-gene-transcription-through-regulating-taf9-deacetylation-and-transcription-factor-iid-assembly
#18
Wei Jian, Bowen Yan, Suming Huang, Yi Qiu
Histone acetyltransferases and histone deacetylases (HDACs) are important epigenetic coregulators. It has been thought that HDACs associate with corepressor complexes and repress gene transcription; however, in this study, we have found that PU.1-a key master regulator for hematopoietic self-renewal and lineage specification-requires HDAC activity for gene activation. Deregulated PU.1 gene expression is linked to dysregulated hematopoiesis and the development of leukemia. In this study, we used erythroid differentiation as a model to analyze how the PU...
June 1, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28559013/establishment-and-genomic-characterization-of-primary-salivary-duct-carcinoma-cell-line
#19
Jie Li, Yohitsugu Mitani, Pulivarthi H Rao, Laszlo Perlaky, Bin Liu, Randal S Weber, Adel K El-Naggar
OBJECTIVES: To develop and characterize in vitro salivary duct carcinoma as a surrogate for functional studies. MATERIALS AND METHODS: Cells were dispersed from tumor tissue fragments under sterile conditions in RPMI media. Disassociated cells were cultivated, immortalized with hTERT and propagated for more than 100 passages. Morphologic, linage, cytogenetic and genomic analyses were performed on different passages of cell line and primary tumor. Soft agar growth was performed...
June 2017: Oral Oncology
https://www.readbyqxmd.com/read/28557973/cerebrospinal-fluid-dissemination-and-neoplastic-meningitis-in-primary-brain-tumors
#20
Sajeel Chowdhary, Sherri Damlo, Marc C Chamberlain
BACKGROUND: Neoplastic meningitis, also known as leptomeningeal disease, affects the entire neuraxis. The clinical manifestations of the disease may affect the cranial nerves, cerebral hemispheres, or the spine. Because of the extent of disease involvement, treatment options and disease staging should involve all compartments of the cerebrospinal fluid (CSF) and subarachnoid space. Few studies of patients with primary brain tumors have specifically addressed treatment for the secondary complication of neoplastic meningitis...
January 2017: Cancer Control: Journal of the Moffitt Cancer Center
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