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https://www.readbyqxmd.com/read/29325213/a-new-colorimetric-platform-for-protein-detection-based-on-recognition-induced-cascade-of-polymeric-nanoparticles-disassembly
#1
Fang Fang, Jin Liu, Yushu Li, Junjiao Yang, Jing Yang
Despite great progress, it is still of high interest to explore new homogeneous assays for simple, visual, and selective protein detection. Herein, one new colorimetric sensor has been developed for visual detection of protein by using polymeric micelles as a sensing scaffold and the molecular recognition between protein and the ligand on the surface of the polymeric micelles as the driving force to trigger the readout of the detection signal. The polymeric micelles formed via the self-assembly of the amphiphilic block polymer biotin-labeled poly(ethylene glycol)-block-poly(3-acryl aminophenylboronic acid) are endowed with colorful feature by incorporation of alizarin red S (ARS) into the hydrophobic core...
January 11, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29285281/activation-of-nrf2-by-mind4-17-protects-osteoblasts-from-hydrogen-peroxide-induced-oxidative-stress
#2
Shiguang Guo, Hao-Dong Fei, Feng Ji, Feng-Li Chen, Yue Xie, Shou-Guo Wang
MIND4-17 is a recently developed NF-E2-related factor 2 (Nrf2) activator, which uniquely causes Nrf2 disassociation from Keap1. Here, we showed that pretreatment with MIND4-17 significantly inhibited hydrogen peroxide (H2O2)-induced viability reduction of primary osteoblasts and OB-6 osteoblastic cells. Meanwhile, MIND4-17 inhibited both apoptotic and non-apoptotic osteoblast cell death by H2O2. MIND4-17 treatment induced Keap1-Nrf2 disassociation, causing Nrf2 stabilization, accumulation and nuclear translocation in osteoblasts, leading to transcription of several Nrf2-dependent genes, including heme oxygenase 1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), γ-glutamylcysteine synthetase modifier subunit (GCLM) and catalytic subunit (GCLC)...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29281729/histone-demethylase-lsd1-restricts-influenza-a-virus-infection-by-erasing-ifitm3-k88-monomethylation
#3
Jiaoyu Shan, Binbin Zhao, Zhao Shan, Jia Nie, Rong Deng, Rui Xiong, Andy Tsun, Weiqi Pan, Hanzhi Zhao, Ling Chen, Ying Jin, Zhikang Qian, Kawing Lui, Rui Liang, Dan Li, Bing Sun, Dimitri Lavillette, Ke Xu, Bin Li
The histone demethylase LSD1 has been known as a key transcriptional coactivator for DNA viruses such as herpes virus. Inhibition of LSD1 was found to block viral genome transcription and lytic replication of DNA viruses. However, RNA virus genomes do not rely on chromatin structure and histone association, and the role of demethylase activity of LSD1 in RNA virus infections is not anticipated. Here, we identify that, contrary to its role in enhancing DNA virus replication, LSD1 limits RNA virus replication by demethylating and activating IFITM3 which is a host restriction factor for many RNA viruses...
December 27, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29242538/nuclear-rnf2-inhibits-interferon-function-by-promoting-k33-linked-stat1-disassociation-from-dna
#4
Shuo Liu, Minghong Jiang, Wendie Wang, Wei Liu, Xiaoqi Song, Zhongfei Ma, Shikun Zhang, Lun Liu, Yin Liu, Xuetao Cao
Prolonged activation of interferon-STAT1 signaling is closely related to inflammatory autoimmune disorders, and therefore the identification of negative regulators of these pathways is important. Through high-content screening of 115 mouse RING-domain E3 ligases, we identified the E3 ubiquitin ligase RNF2 as a potent inhibitor of interferon-dependent antiviral responses. RNF2 deficiency substantially enhanced interferon-stimulated gene (ISG) expression and antiviral responses. Mechanistically, nuclear RNF2 directly bound to STAT1 after interferon stimulation and increased K33-linked polyubiquitination of the DNA-binding domain of STAT1 at position K379, in addition to promoting the disassociation of STAT1/STAT2 from DNA and consequently suppressing ISG transcription...
January 2018: Nature Immunology
https://www.readbyqxmd.com/read/29233021/amitriptyline-modulated-ca2-signaling-and-induced-ca2-independent-cell-viability-in-human-osteosarcoma-cells
#5
T Lu, C-T Chou, W-Z Liang, C-C Kuo, I-L Chen, J-L Wang, C-R Jan
Amitriptyline is a widely used tricyclic antidepressant, which acts primarily as a serotonin-norepinephrine reuptake inhibitor. This study examined the effect of amitriptyline on Ca2+ homeostasis and its related mechanism in MG63 human osteosarcoma cells. Amitriptyline evoked cytosolic-free Ca2+ concentrations ([Ca2+]i) rises concentration dependently. Amitriptyline-evoked Ca2+ entry was confirmed by Mn2+-induced quench of fura-2 fluorescence. This entry was inhibited by Ca2+ entry modulators nifedipine, econazole, SKF96365, the protein kinase C (PKC) activator phorbol 12-myristate 13 acetate but was not affected by the PKC inhibitor GF109203X...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29232146/switchable-photoacoustic-intensity-of-methylene-blue-via-sodium-dodecyl-sulfate-micellization
#6
Junxin Wang, Ching-Yu Lin, Colman Moore, Anamik Jhunjhunwala, Jesse V Jokerst
The interaction between methylene blue (MB) and sodium dodecyl sulfate (SDS) has been widely studied spectroscopically, but details about their interactions are limited. Here, we combined photoacoustic (PA) imaging with nanoparticle tracking analysis (NTA) and spectroscopy to further elucidate this interaction. PA imaging of 0.05 mM MB showed a 492-fold increase in intensity upon addition of 3.47 mM SDS. Higher concentrations above SDS's critical micelle concentration (CMC) at 8.67 mM decreased the PA intensity by 54-fold...
December 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29188176/nano-sized-indocyanine-green-j-aggregate-as-a-one-component-theranostic-agent
#7
Renfa Liu, Jie Tang, Yunxue Xu, Yiming Zhou, Zhifei Dai
The development of biocompatiable efficient photothermal coupling agent (PCA) for image-guided photothermal therapy of cancer has gained increasing interests in recent years. Although various PCAs have been developed, the clinical translations of these materials have been largely hindered by the potential biosafety issues and challenges of scaling-up manufactures. In this research, we proposed nano-sized indocyanine green (ICG) J-aggregate (IJA) as a promising PCA which is fabricated by a very facile method using clinical-approved ICG as the only excipient...
2017: Nanotheranostics
https://www.readbyqxmd.com/read/29183342/the-ph-stability-of-foot-and-mouth-disease-virus
#8
REVIEW
Hong Yuan, Pinghua Li, Xueqing Ma, Zengjun Lu, Pu Sun, Xingwen Bai, Jing Zhang, Huifang Bao, Yimei Cao, Dong Li, Yuanfang Fu, Yingli Chen, Qifeng Bai, Jie Zhang, Zaixin Liu
ᅟ: This review summarized the molecular determinants of the acid stability of FMDV in order to explore the uncoating mechanism of FMDV and improve the acid stability of vaccines. BACKGROUND: The foot-and-mouth disease virus (FMDV) capsid is highly acid labile and tends to dissociate into pentameric subunits at acidic condition to release viral RNA for initiating virus replication. However, the acid stability of virus capsid is greatly required for the maintenance of intact virion during the process of virus culture and vaccine production...
November 28, 2017: Virology Journal
https://www.readbyqxmd.com/read/29163044/partial-aminoglycoside-lesions-in-vestibular-epithelia-reveal-broad-sensory-dysfunction-associated-with-modest-hair-cell-loss-and-afferent-calyx-retraction
#9
David R Sultemeier, Larry F Hoffman
Although the effects of aminoglycoside antibiotics on hair cells have been investigated for decades, their influences on the dendrites of primary afferent neurons have not been widely studied. This is undoubtedly due to the difficulty in disassociating pathology to dendritic processes from that resulting from loss of the presynaptic hair cell. This was overcome in the present investigation through development of a preparation using Chinchilla laniger that enabled direct perilymphatic infusion. Through this strategy we unmasked gentamicin's potential effects on afferent calyces...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29112302/tmd1-domain-and-crac-motif-determine-the-association-and-disassociation-of-mxirt1-with-detergent-resistant-membranes
#10
Song Tan, Peng Zhang, Wei Xiao, Bing Feng, Lan-You Chen, Shuang Li, Peng Li, Wei-Zhong Zhao, Xiao-Ting Qi, Li-Ping Yin
Iron is essential for most living organisms. The iron-regulated transporter1 (IRT1) plays a major role in iron uptake in roots, and its trafficking from endoplasmic reticulum (ER) to plasma membrane (PM) is tightly coordinated with changes in iron environment. However, studies on IRT1 response are limited. Here, we report that Malus xiaojinesis IRT1 (MxIRT1) associates with detergent-resistant membranes (DRMs, a biochemical counterpart of PM microdomains), whereas the PM microdomains are known platforms for signal transduction in the PM...
November 7, 2017: Traffic
https://www.readbyqxmd.com/read/29111371/pharmacodynamics-in-alzheimer-s-disease-model-rats-of-a-bifunctional-peptide-with-the-potential-to-accelerate-the-degradation-and-reduce-the-toxicity-of-amyloid-%C3%AE-cu-fibrils
#11
Dan Wang, Qian Zhang, Xiaoyu Hu, Wei Wang, Xushan Zhu, Zhi Yuan
The accumulation of the extracellular β-amyloid (Aβ) aggregates with metal ions in conjunction with reactive oxygen species (ROS) is closely related to the pathogenesis of Alzheimer's disease (AD). Accounting on Cu ions chelating of our previously designed bifunctional peptide GGHRYYAAFFARR (GR) as well as Aβ-Cu fibrils (fAβ-Cu) dissociation potentials, we report herein an efficient route to synthetically minimize ROS toxicity and degrade fAβ-Cu. It is worth mentioning that GR combines the metal chelating agent GGH and β-sheet breaker RYYAAFFARR (RR)...
October 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29108762/alveolar-microlithiasis-and-its-distinctive-clinical-and-radiological-disassociation
#12
Laura Cuesta Lujano, Álvaro Gutiérrez Domingo, Luis Fernández Ollero
No abstract text is available yet for this article.
November 3, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/29097744/alleviative-effects-of-fluoxetine-on-depressive-like-behaviors-by-epigenetic-regulation-of-bdnf-gene-transcription-in-mouse-model-of-post-stroke-depression
#13
Hui-Juan Jin, Lei Pei, Ya-Nan Li, Hui Zheng, Shuai Yang, Yan Wan, Ling Mao, Yuan-Peng Xia, Quan-Wei He, Man Li, Zhen-Yu Yue, Bo Hu
Fluoxetine, one of the selective serotonin reuptake inhibitor (SSRI) antidepressants, has been thought to be effective for treating post-stroke depression (PSD). Recent work has shown that fluoxetine may exert an antidepressive effect through increasing the level of brain-derived neurotrophic factor (BDNF), but the underlying mechanism still remains unclear. In the present study, we successfully established the PSD model using male C57BL/6 J mice by photothrombosis of the left anterior cortex combined with isolatied-housing conditions...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29091765/low-grade-astrocytoma-mutations-in-idh1-p53-and-atrx-cooperate-to-block-differentiation-of-human-neural-stem-cells-via-repression-of-sox2
#14
Aram S Modrek, Danielle Golub, Themasap Khan, Devin Bready, Jod Prado, Christopher Bowman, Jingjing Deng, Guoan Zhang, Pedro P Rocha, Ramya Raviram, Charalampos Lazaris, James M Stafford, Gary LeRoy, Michael Kader, Joravar Dhaliwal, N Sumru Bayin, Joshua D Frenster, Jonathan Serrano, Luis Chiriboga, Rabaa Baitalmal, Gouri Nanjangud, Andrew S Chi, John G Golfinos, Jing Wang, Matthias A Karajannis, Richard A Bonneau, Danny Reinberg, Aristotelis Tsirigos, David Zagzag, Matija Snuderl, Jane A Skok, Thomas A Neubert, Dimitris G Placantonakis
Low-grade astrocytomas (LGAs) carry neomorphic mutations in isocitrate dehydrogenase (IDH) concurrently with P53 and ATRX loss. To model LGA formation, we introduced R132H IDH1, P53 shRNA, and ATRX shRNA into human neural stem cells (NSCs). These oncogenic hits blocked NSC differentiation, increased invasiveness in vivo, and led to a DNA methylation and transcriptional profile resembling IDH1 mutant human LGAs. The differentiation block was caused by transcriptional silencing of the transcription factor SOX2 secondary to disassociation of its promoter from a putative enhancer...
October 31, 2017: Cell Reports
https://www.readbyqxmd.com/read/29061846/a-transcriptional-coregulator-spin-doc-attenuates-the-coactivator-activity-of-spindlin1
#15
Narkhyun Bae, Min Gao, Xu Li, Tolkappiyan Premkumar, Gianluca Sbardella, Junjie Chen, Mark T Bedford
Spindlin1 (SPIN1) is a transcriptional coactivator with critical functions in embryonic development and emerging roles in cancer. SPIN1 harbors three Tudor domains, two of which engage the tail of histone H3 by reading the H3-Lys-4 trimethylation (H3K4me3) and H3-Arg-8 asymmetric dimethylation (H3R8me2a) marks. To gain mechanistic insight into how SPIN1 functions as a transcriptional coactivator, here we purified its interacting proteins. We identified an uncharacterized protein (C11orf84), which we renamed SPIN1 docking protein (SPIN-DOC), that directly binds SPIN1 and strongly disrupts its histone methylation reading ability, causing it to disassociate from chromatin...
October 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29058592/ureteral-reconstruction-using-a-tapered-non-vascularized-bladder-graft-an-experimental-study-in-a-canine-animal-model
#16
Lujia Zou, Shanhua Mao, Shenghua Liu, Limin Zhang, Tian Yang, Yun Hu, Qiang Ding, Haowen Jiang
BACKGROUND: Reconstruction of ureteral defects and strictures remains problematic for urologists. We aimed to investigate the possibility of a tapered non-vascularized bladder graft as a novel substitute for ureteral reconstruction. METHODS: This experimental study was conducted on nine beagles. Under general anesthesia, a full-thickness graft with 5-6 cm in length was disassociated from the anterior upper wall of the bladder, and tapered into 1/3 to 1/2 thickness, remaining the urothelial surface...
October 23, 2017: BMC Urology
https://www.readbyqxmd.com/read/29050336/transcriptional-downregulation-of-microrna-19a-by-ros-production-and-nf-%C3%AE%C2%BAb-deactivation-governs-resistance-to-oxidative-stress-initiated-apoptosis
#17
Jun Hong, Ying Wang, Bang-Chuan Hu, Liang Xu, Jing-Quan Liu, Min-Hua Chen, Jin-Zhu Wang, Fang Han, Yang Zheng, Xu Chen, Qian Li, Xiang-Hong Yang, Ren-Hua Sun, Shi-Jing Mo
Cell apoptosis is one of the main pathological alterations during oxidative stress (OS) injury. Previously, we corroborated that nuclear factor-κB (NF-κB) transactivation confers apoptosis resistance against OS in mammalian cells, yet the underlying mechanisms remain enigmatic. Here we report that microRNA-19a (miR-19a) transcriptionally regulated by reactive oxygen species (ROS) production and NF-κB deactivation prevents OS-initiated cell apoptosis through cylindromatosis (CYLD) repression. CYLD contributes to OS-initiated cell apoptosis, for which NF-κB deactivation is essential...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29035037/controlling-superwettability-by-microstructure-and-surface-energy-manipulation-on-three-dimensional-substrates-for-versatile-gravity-driven-oil-water-separation
#18
Hao-Yang Mi, Xin Jing, Han-Xiong Huang, Lih-Sheng Turng
Superwettable materials have gained tremendous attention because of their special wetting abilities. The key to obtaining and tuning superwettability is to precisely control the interfacial microstructures and surface energies of materials. Herein, we propose a novel approach to controlling the superwettability of three-dimensional foams. The surface microstructure was manipulated by the layer-by-layer covalent grafting of multidimensional nanoparticles (e.g., silica, carbon nanotubes, and graphene oxide), and the surface energy was tailored by grafting chemicals with different functional groups...
October 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29024529/catalytic-conductive-bipolar-membrane-interfaces-through-layer-by-layer-deposition-for-the-design-of-membrane-integrated-artificial-photosynthesis-systems
#19
Michael B McDonald, Michael S Freund, Paula T Hammond
In the presence of an electric field, bipolar membranes (BPMs) are capable of initiating water disassociation (WD) within the interfacial region, which can make water splitting for renewable energy in the presence of a pH gradient possible. In addition to WD catalytic efficiency, there is also the need for electronic conductivity in this region for membrane-integrated artificial photosynthesis (AP) systems. Graphene oxide (GO) was shown to catalyze WD and to be controllably reduced, which resulted in electronic conductivity...
October 10, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28980690/rational-design-of-food-grade-polyelectrolyte-complex-coacervate-for-encapsulation-and-enhanced-oral-delivery-of-oenothein-b
#20
Yaqi Lan, Li Wang, Sufang Cao, Yinger Zhong, Yunqi Li, Yong Cao, Lichao Zhao
Oenothein B (OeB), a dimeric macrocyclic ellagitannin isolated from eucalyptus leaves has been demonstrated as a promising natural bioactive compound for its remarkable antitumor, antioxidant, anti-inflammatory and immunomodulating effects. Unfortunately, early study indicates that OeB has quite low bioaccessibility for oral consumption due to their susceptibility to decomposition both in vitro and in vivo. Herein, we report the design and synthesis of food-grade polyelectrolyte complex coacervate using caseinophosphopeptides (CPPs) and chitosan (CS) to encapsulate OeB for enhanced protection through gastrointestinal (GI) tract...
October 5, 2017: Food & Function
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