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https://www.readbyqxmd.com/read/28077337/stress-hormone-epinephrine-adrenaline-and-norepinephrine-noradrenaline-effects-on-the-anaerobic-bacteria
#1
REVIEW
Lyudmila Boyanova
Microbial endocrinology is a relatively new research area that already encompasses the anaerobes. Stress hormones, epinephrine and norepinephrine, can affect the growth of anaerobic bacteria such as Fusobacterium nucleatum, Prevotella spp., Porhyromonas spp., Tanerella forsythia and Propionibacterium acnes and can increase virulence gene expression, iron acquisition and many virulence factors of some anaerobic species such as Clostridium perfringens, Porphyromonas gingivalis and Brachyspira pilosicoli. Epinephrine and norepinephrine effects can lead to a growth increase or decrease, or no effect on the growth of the anaerobes...
January 8, 2017: Anaerobe
https://www.readbyqxmd.com/read/28050984/evaluation-of-bacteriological-profile-in-the-apical-root-segment-of-the-patients-with-primary-apical-periodontitis
#2
Aravind Tatikonda, N Sudheep, Krishna P Biswas, K Gowtham, Sudarshan Pujari, Padam Singh
INTRODUCTION: Apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. Literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. The reason for this disparity might be the technique used for collecting the samples from the pulp canals. Hence, we carried out the present study to evaluate the microbial flora in the apical part of the roots with necrotic pulp canals...
January 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28045107/collateral-damage-insights-into-bacterial-mechanisms-that-predispose-host-cells-to-cancer
#3
Aurélie Gagnaire, Bertrand Nadel, Didier Raoult, Jacques Neefjes, Jean-Pierre Gorvel
Infections are estimated to contribute to 20% of all human tumours. Viruses are known to induce cell transformation, but evidence has also linked bacteria, such as Helicobacter pylori and Salmonella enterica subsp. enterica serovar Typhi, to different cancer types. In addition, Chlamydia trachomatis, Fusobacterium nucleatum and Bacteroides fragilis are associated with the development of cancer, although a causal relationship has not yet been established. Bacterial effectors such as colibactin and the virulence factor cytotoxin-associated gene A (CagA) can promote cancer directly by influencing host cell signalling cascades, such as the WNT and ataxia-telangiectasia mutated (ATM) pathways, or indirectly by inducing tissue damage and inflammatory responses...
January 3, 2017: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/28040511/fusobacterium-necrophorum-tonsillitis-with-mild-case-of-lemierre-s-syndrome
#4
Pavan Kumar Panchavati, Bhavini Kar, Ali Hassoun, Robert M Centor
No abstract text is available yet for this article.
December 28, 2016: Anaerobe
https://www.readbyqxmd.com/read/28034636/detection-of-fusobacterium-nucleatum-in-two-cases-of-empyema-and-lung-abscess-using-paromomycin-vancomycin-supplemented-brucella-hk-agar
#5
Kentaro Nagaoka, Katsunori Yanagihara, Yoshitomo Morinaga, Shigeru Kohno
Fusobacterium nucleatum was found in patients with empyema or pulmonary abscess, using paromomycin-vancomycin Brucella HK agar. In vitro examination revealed that growth of the strains differed significantly in different media. Clinicians should be aware that suboptimal F. nucleatum cultivation methods may result in an underestimation of its frequency.
December 26, 2016: Anaerobe
https://www.readbyqxmd.com/read/28028421/the-use-of-different-irrigation-techniques-to-decrease-bacterial-loads-in-healthy-and-diabetic-patients-with-asymptomatic-apical-periodontitis
#6
Mai Ghoneim, Shehab ElDin Saber, Tarek El-Badry, Maram Obeid, Nehal Hassib
BACKGROUND: Diabetes mellitus is a multisystem disease which weakens the human's immunity. Subsequently, it worsens the sequelae of apical periodontitis by raising a fierce bacterial trait due to the impaired host response. AIM: This study aimed to estimate bacterial reduction after using different irrigation techniques in systemically healthy and diabetic patients with asymptomatic apical periodontitis. MATERIAL AND METHODS: Enterococcus faecalis, Peptostreptococcus micros, and Fusobacterium necleatum bacteria were chosen, as they are the most common and prevailing strains found in periodontitis...
December 15, 2016: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28013413/selective-persistence-of-propionibacterium-species-fma5-following-sealing-of-infected-dentinal-matrix
#7
M A Nadkarni, K Angner, N Hunter
The objective of the present study was to test the hypothesis that nutrient deprivation by effective isolation should inactivate causative saccharolytic bacteria occupying carious lesions. Vital maxillary third molar teeth were prepared by removing only the superficial necrotic material, leaving behind infected dentinal matrix, before the cavity was sealed with glass ionomer cement (GIC). Before sealing, lesions were biopsied to provide reference bacterial DNA for microbial analysis. After an interval of 10-12 months, the teeth were extracted and, after careful removal of GIC restoration, the underlying dentine was biopsied again for post-treatment microbial analysis...
December 24, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27999400/comparative-study-on-the-characteristics-of-weissella-cibaria-cmu-and-probiotic-strains-for-oral-care
#8
Hye-Jin Jang, Mi-Sun Kang, Sung-Hun Yi, Ji-Young Hong, Sang-Pil Hong
Probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. The present work was conducted to compare the characteristics of oral care probiotics: Weissella cibaria CMU (Chonnam Medical University) and four commercial probiotic strains. Survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and inhibition of volatile sulfur compounds were evaluated...
December 20, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27994578/comparative-in-silico-analysis-of-butyrate-production-pathways-in-gut-commensals-and-pathogens
#9
Swadha Anand, Harrisham Kaur, Sharmila S Mande
Biosynthesis of butyrate by commensal bacteria plays a crucial role in maintenance of human gut health while dysbiosis in gut microbiome has been linked to several enteric disorders. Contrastingly, butyrate shows cytotoxic effects in patients with oral diseases like periodontal infections and oral cancer. In addition to these host associations, few syntrophic bacteria couple butyrate degradation with sulfate reduction and methane production. Thus, it becomes imperative to understand the distribution of butyrate metabolism pathways and delineate differences in substrate utilization between pathogens and commensals...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27988390/low-antibiotic-resistance-among-anaerobic-gram-negative-bacteria-in-periodontitis-5-years-following-metronidazole-therapy
#10
G Dahlen, H R Preus
The objective of this study was to assess antibiotic susceptibility among predominant Gram-negative anaerobic bacteria isolated from periodontitis patients who 5 years prior had been subject to mechanical therapy with or without adjunctive metronidazole. One pooled sample was taken from the 5 deepest sites of each of 161 patients that completed the 5 year follow-up after therapy. The samples were analyzed by culture. A total number of 85 anaerobic strains were isolated from the predominant subgingival flora of 65/161 patient samples, identified, and tested for antibiotic susceptibility by MIC determination...
December 14, 2016: Anaerobe
https://www.readbyqxmd.com/read/27982490/rt-pcr-quantification-of-periodontal-pathogens-in-crack-users-and-non-users
#11
M Casarin, R P Antoniazzi, R A Vaucher, C A Feldens, F B Zanatta
OBJECTIVE: To compare counts of Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis and Fusobacterium nucleatum between crack users and non-users. MATERIALS AND METHODS: A cross-sectional study was conducted involving seventy-four crack cocaine users and eighty-one non-users matched for age, gender and tobacco use. Demographic and clinical variables were analysed. Subgingival bacterial samples were collected from four sites with the greatest probing depths and were analysed using real-time polymerase chain reaction...
November 5, 2016: Oral Diseases
https://www.readbyqxmd.com/read/27982237/murine-experimental-root-canal-infection-cytokine-expression-in-response-to-f-nucleatum-and-e-faecalis
#12
Andressa Lamari Reis, Ricardo Reis Oliveira, Warley Luciano Fonseca Tavares, Thamyris Duque Silva Saldanha, Luiz de Macêdo Farias, Leda Quércia Vieira, Antônio Paulino Ribeiro
The aim of this study was to evaluate the gene expression of proinflammatory (RANKL, TNF-a and IFN-g) and regulatory (TGF-b and IL-10) cytokines as reaction to experimental infection by mono or bi-association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E. faecalis, either in mono- or bi-association were inoculated into the root canal system (RCS) of Balb/c mice. Animals were sacrificed at 10 and 20 days after infection and periapical tissues surrounding the root were collected...
September 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27977559/methicillin-resistant-staphylococcus-aureus-as-a-cause-of-lemierre-s-syndrome
#13
Ravi H Jariwala, Swetha Srialluri, Maria Z Huang, Suresh B Boppana
Lemierre's syndrome (LS) or jugular vein suppurative thrombophlebitis is well described in literature. The organisms most often responsible are Fusobacterium necrophorum or anaerobic flora. We present a case of LS with an atypical microbiologic cause, methicillin-resistant Staphylococcus aureus (MRSA). We also present retrospective review of all LS cases from our institution and identified two additional children with LS caused by MRSA.
December 12, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27976528/streptococcus-mutans-spap-binds-to-radd-of-fusobacterium-nucleatum-ssp-polymorphum
#14
Lihong Guo, Bhumika Shokeen, Xuesong He, Wenyuan Shi, Renate Lux
Adhesin-mediated bacterial interspecies interactions are important elements in oral biofilm formation. They often occur on a species-specific level, which could determine health- or disease association of a biofilm community. Among the key players involved in these processes are the ubiquitous fusobacteria that have been recognized for their ability to interact with numerous different binding partners. Fusobacterial interactions with Streptococcus mutans, an important oral cariogenic pathogen, have previously been described but most studies focused on binding to non-mutans streptococci and specific cognate adhesin pairs remain to be identified...
December 15, 2016: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/27966414/-design-of-artificial-foetor-flatus-based-on-bacterial-volatile-compounds
#15
Ulrik Stenz Justesen
INTRODUCTION: Excessive flatulence can be a huge social problem. The purpose of this study was to design artificial flatus from bacterial volatile compounds to stimulate research into neutralizing measures. MATERIAL AND METHODS: Anaerobic bacteria, representing a broad spectrum, from a recognized international culture collection were included. The strains were incubated in an anaerobic jar. After 24 hours the lid was removed, and the odour was evaluated by a specialist in clinical microbiology...
December 12, 2016: Ugeskrift for Laeger
https://www.readbyqxmd.com/read/27965635/photoinactivation-using-visible-light-plus-water-filtered-infrared-a-vis-wira-and-chlorine-e6-ce6-eradicates-planktonic-periodontal-pathogens-and-subgingival-biofilms
#16
Ali Al-Ahmad, Aleksander Walankiewicz, Elmar Hellwig, Marie Follo, Christian Tennert, Annette Wittmer, Lamprini Karygianni
Alternative treatment methods for pathogens and microbial biofilms are required due to the widespread rise in antibiotic resistance. Antimicrobial photodynamic therapy (aPDT) has recently gained attention as a novel method to eradicate pathogens. The aim of this study was to evaluate the antimicrobial effects of a novel aPDT method using visible light (vis) and water infiltrated infrared A (wIRA) in combination with chlorine e6 (Ce6) against different periodontal pathogens in planktonic form and within in situ subgingival oral biofilms...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27936055/microbial-diversity-of-source-and-point-of-use-water-in-rural-haiti-a-pyrosequencing-based-metagenomic-survey
#17
Nabanita Mukherjee, Debra Bartelli, Cyril Patra, Bhavin V Chauhan, Scot E Dowd, Pratik Banerjee
Haiti endures the poorest water and sanitation infrastructure in the Western Hemisphere, where waterborne diseases cause significant morbidity and mortality. Most of these diseases are reported to be caused by waterborne pathogens. In this study, we examined the overall bacterial diversity of selected source and point-of-use water from rural areas in Central Plateau, Haiti using pyrosequencing of 16s rRNA genes. Taxonomic composition of water samples revealed an abundance of Firmicutes phyla, followed by Proteobacteria and Bacteroidetes...
2016: PloS One
https://www.readbyqxmd.com/read/27924595/quantitative-analysis-of-periodontal-pathogens-using-real-time-polymerase-chain-reaction-pcr
#18
Mª José Marin, Elena Figuero, David Herrera, Mariano Sanz
The quantitative polymerase chain reaction (qPCR) is a variant of PCR aimed to detect and quantify a targeted DNA molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated PCR product. This chapter presents the detailed procedures for quantification of different periodontal pathogens (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, and Fusobacterium spp...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909688/the-danger-signal-extracellular-atp-is-an-inducer-of-fusobacterium-nucleatum-biofilm-dispersal
#19
Qinfeng Ding, Kai Soo Tan
Plaque biofilm is the primary etiological agent of periodontal disease. Biofilm formation progresses through multiple developmental stages beginning with bacterial attachment to a surface, followed by development of microcolonies and finally detachment and dispersal from a mature biofilm as free planktonic bacteria. Tissue damage arising from inflammatory response to biofilm is one of the hallmark features of periodontal disease. A consequence of tissue damage is the release of ATP from within the cell into the extracellular space...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27899684/high-risk-periodontal-pathogens-contribute-to-the-pathogenesis-of-atherosclerosis
#20
REVIEW
Bradley Field Bale, Amy Lynn Doneen, David John Vigerust
Periodontal disease (PD) is generated by microorganisms. These microbes can enter the general circulation causing a bacteraemia. The result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. Level A evidence supports that PD is independently associated with arterial disease. PD is a common chronic condition affecting the majority of Americans 30 years of age and older. Atherosclerosis remains the largest cause of death and disability. Studies indicate that the adverse cardiovascular effects from PD are due to a few putative or high-risk bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola or Fusobacterium nucleatum There are three accepted essential elements in the pathogenesis of atherosclerosis: lipoprotein serum concentration, endothelial permeability and binding of lipoproteins in the arterial intima...
November 29, 2016: Postgraduate Medical Journal
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