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Francesca Tocchioni, Chiara Tani, Laura Bartolini, Maria Moriondo, Francesco Nieddu, Patrizia Pecile, Chiara Azzari, Antonio Messineo, Marco Ghionzoli
Bacterial growth of peritoneal fluid specimens obtained during surgical procedures for acute appendicitis may be useful to optimize further antibiotic therapy in complicated cases. DNA amplification represents a fast technique to detect microbial sequences. We aimed to compare the potential of DNA amplification versus traditional bacterial growth culture highlighting advantages and drawbacks in a surgical setting. Peritoneal fluid specimens were collected during surgery from 36 children who underwent appendectomy between May and December 2012...
September 19, 2016: Pediatric Reports
Xiao-Yu Hu, Yu-Fei Yao, Bo-Miao Cui, Jun Lv, Xin Shen, Biao Ren, Ming-Yun Li, Qiang Guo, Rui-Jie Huang, Yan Li
OBJECTIVE: To analyze the whole microbial structure in a case of rampant caries to provide evidence for its prevention and treatment. METHODS: Clinical samples including blood, supragingival plaque, plaque in the caries cavity, saliva, and mucosal swabs were collected with the patient's consent. The blood sample was sent for routine immune test, and the others samples were stained using Gram method and cultured for identifying colonies and 16S rRNA sequencing. DNA was extracted from the samples and tested for the main cariogenic bacterium (Streptococcus mutans) with qPCR, and the whole microbial structure was analyzed using DGGE...
October 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
T Didbaridze, L Saginashvili, L Akhmeteli, B Iremashvili, N Gogokhia
This study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. Our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (API20E, API Staph, API Strep, API Ana, BioMerieux). Rapid tests for detection of oxidase and catalase activity were also used. Susceptibility of microorganisms to antibiotics was defined by the disc-diffusion method using standard discs (EUCAST guidelines 2015) according to Clinical Laboratory Standard Institute (CLSI) protocols (ATB strips: ATB G, ATB Staph, ATBANA, ATBPse, ATBStrep...
September 2016: Georgian Medical News
Kensuke Yamamura, Yoshifumi Baba, Shigeki Nakagawa, Kosuke Mima, Keisuke Miyake, Kenichi Nakamura, Hiroshi Sawayama, Koichi Kinoshita, Takatsugu Ishimoto, Masaaki Iwatsuki, Yasuo Sakamoto, Yoichi Yamashita, Naoya Yoshida, Masayuki Watanabe, Hideo Baba
PURPOSE: Fusobacterium nucleatum (F. nucleatum) is a component of the human microbiome that primarily inhabits the oral cavity. It causes periodontal disease and has also been implicated in the development of human cancers. Although there are several reports of the relationship between F. nucleatum and the clinical outcome in human cancers, its prognostic significance in esophageal cancer remains unclear. EXPERIMENTAL DESIGN: We quantified F. nucleatum DNA in 325 resected esophageal cancer specimens by qPCR...
October 21, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Şivge Kurgan, Shevali Kansal, Daniel Nguyen, Danielle Stephens, Yannis Koroneos, Hatice Hasturk, Thomas E Van Dyke, Alpdogan Kantarci
BACKGROUND: Neutrophil function is critical for the initiation and progression of infecto-inflammatory diseases. Key quorum-sensing plaque bacteria such as Fusobacterium nucleatum act as bridging species between early and late colonizer pathogens such as Porphyromonas gingivalis as the biofilm ages and periodontal inflammation increases. This study was designed to determine the impact of different F. nucleatum strains on neutrophil function. MATERIALS AND METHODS: HL-60 cells were differentiated into neutrophil-like cells and cultured with F...
October 20, 2016: Journal of Periodontology
Julia C Schmidt, Monika Astasov-Frauenhoffer, Tuomas Waltimo, Roland Weiger, Clemens Walter
OBJECTIVES: The objective of this study was to evaluate the efficacy of four different side-to-side toothbrushes and the impact of various brushing parameters on noncontact biofilm removal in an adjustable interdental space model. MATERIALS AND METHODS: A three-species biofilm, consisting of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus sanguinis, was formed in vitro on protein-coated titanium disks using a flow chamber combined with a static biofilm growth model...
October 19, 2016: Clinical Oral Investigations
Marika Rai, David Spratt, Paola R Gomez-Pereira, Jay Patel, Sean P Nair
Oral malodour is a common condition which affects a large proportion of the population, resulting in social, emotional and psychological stress. Certain oral bacteria form a coating called a biofilm on the tongue dorsum and degrade organic compounds releasing volatile sulfur compounds that are malodourous. Current chemical treatments for oral malodour such as mouthwashes containing chlorhexidine or essential oils, are not sufficiently effective at reducing the bacterial load on the tongue. One potential alternative to current chemical treatments for oral malodour is the use of light activated antimicrobial agents (LAAAs), which display no toxicity or antimicrobial activity in the dark, but when exposed to light of a specific wavelength produce reactive oxygen species which induce damage to target cells in a process known as photodynamic inactivation...
October 18, 2016: Journal of Breath Research
Li-Wan Lee, Ya-Ling Lee, Sheng-Huang Hsiao, Hung-Pin Lin
BACKGROUND/PURPOSE: Bacteria in the tooth root canal may cause apical periodontitis. This study examined the bacterial species present in the apical root canal of teeth with apical periodontitis. Antibiotic sensitivity tests were performed to evaluate whether these identified bacterial species were susceptible to specific kinds of antibiotics. METHODS: Selective media plating and biochemical tests were used first to detect the bacterial species in samples taken from the apical portion of root canals of 62 teeth with apical periodontitis...
October 10, 2016: Journal of the Formosan Medical Association, Taiwan Yi Zhi
Pauline M Lang, Rogério C Jacinto, Tatiane S Dal Pizzol, Maria Beatriz C Ferreira, Francisco Montagner
Infected root canal or acute apical abscess exudates can harbour several species, including Fusobacterium, Porphyromonas, Prevotella, Parvimonas, Streptococcus, Treponema, Olsenella and not-yet cultivable species. A systematic review and meta-analysis was performed to assess resistance rates to antimicrobial agents in clinical studies that isolated bacteria from acute endodontic infections. Electronic databases and the grey literature were searched up to May 2015. Clinical studies in humans evaluating the antimicrobial resistance of primary acute endodontic infection isolates were included...
September 30, 2016: International Journal of Antimicrobial Agents
Karen Arane, Ran D Goldman
QUESTION: A 2-year-old patient in my practice with acute otitis media that has progressed to mastoiditis with a high fever returns with positive culture results for Fusobacterium What should I do next? ANSWER: Fusobacterium is a genus of anaerobic bacteria. Although Fusobacterium infections are rare, they can become severe if not treated promptly. Appropriate treatment is combination antibiotic therapy consisting of a β-lactam (penicillin, cephalosporin) and an anaerobic antimicrobial agent (metronidazole, clindamycin)...
October 2016: Canadian Family Physician Médecin de Famille Canadien
Letícia Maria Menezes Nóbrega, Francisco Montagner, Adriana Costa Ribeiro, Márcia Alves Pinto Mayer, Brenda Paula Figueiredo de Almeida Gomes
The aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and sequencing. A bacterial genomic library was constructed and bacterial diversity was estimated. The mean number of taxa per canal was 15, ranging from 11 to 21. A total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) were different species and 29 (38...
October 10, 2016: Brazilian Oral Research
John F Antiabong, Mafora G Ngoepe, Adakole S Abechi
AIM: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a readily accessible technology in laboratories across developing nations. The aim of this study was to evaluate a semi-quantitative method for conventional PCR amplicons using digital image analysis of electrophoretic gel. The potential applications are also discussed...
September 2016: Veterinary World
Camila Faria Carrada, Flávia Almeida Ribeiro Scalioni, Dionéia Evangelista Cesar, Karina Lopes Devito, Luiz Cláudio Ribeiro, Rosangela Almeida Ribeiro
OBJECTIVE: To assess and compare salivary periodontopathic bacteria between groups of Down syndrome and non-Down syndrome children and adolescents. MATERIALS AND METHODS: This study included a sample of 30 Down syndrome children and adolescents (G-DS) and 30 age- and sex-matched non-Down syndrome subjects (G-ND). Clinical examination determined the gingival bleeding index (GBI) and plaque index. Unstimulated whole saliva samples were collected from all participants...
2016: PloS One
Stefano Cappanera, Beatrice Tiri, Lavinia M Saraca, Daniela Francisci
Lemierre's syndrome is a dangerous potential sequela of pharyngitis that results in septic thrombophlebitis of the internal jugular vein. A high index of suspicion is needed to consider this diagnosis in the workup of pharyngitis and should be aggressively treated once. Gram-negative anaerobic bacteria, mainly Fusobacterium necrophorum are implicated. Before the antibiotic era, Lemierre's syndrome was common and often fatal. But with the introduction of penicillin in the 1940s, the incidence of the syndrome dropped, and it eventually became known as the "forgotten disease"...
September 2016: Recenti Progressi in Medicina
Simone Stauffacher, Adrian Lussi, Sandor Nietzsche, Klaus W Neuhaus, Sigrun Eick
OBJECTIVES: We wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals. METHODS: Fifty-one human roots were incubated for 8 weeks with monocultured Streptococcus gordonii ATCC 10558, Streptococcus sanguinis ATCC 10556, and with five capnophiles/anaerobes as well as with capnophiles/anaerobes co-cultured with a streptococcal species. Thereafter, bacterial samples were cultured from the inner, middle, and outer third of the root dentine of longitudinally broken teeth (n = 5)...
October 8, 2016: Clinical Oral Investigations
Lori D Racsa, Marlene DeLeon-Carnes, Matthew Hiskey, Jeannette Guarner
16S sequencing on formalin-fixed paraffin-embedded (FFPE) material has been used to identify bacteria when culture-based phenotyping techniques have not worked. The objective of this study was to determine how frequently 16S sequencing used in FFPE material was helpful to clinicians in the diagnosis and treatment of infectious diseases. Requests for testing occurred upon consultation between an infectious disease pathologist and a surgical pathologist or an infectious disease physician. A selected paraffin block from each case was referred for 16S sequencing...
October 4, 2016: Human Pathology
Soo Jin Jeon, Zhengxin Ma, Minyoung Kang, Klibs N Galvão, Kwangcheol Casey Jeong
Uterine disease such as metritis is associated with multiple bacterial infections in the uteri after parturition. However, treatment of metritis is challenging due to considerably high antibiotic treatment failure rate with unknown reason. Recently, chitosan microparticles (CM) have been developed to exert broad spectrum antimicrobial activity against bacterial pathogens, including multi-drug resistant bacteria, without raising CM resistant mutants. In this study, we tested, using metagenomics analysis, if CM maintain strong antimicrobial activity against pathogenic bacteria such as Fusobacteriaceae and Bacteroidaceae in cow uteri and evaluated CM's potency as an alternative antimicrobial agent to cure metritis in cows...
December 2016: Biomaterials
Steffen Bank, Anders Jensen, Hanne Merete Nielsen, Lena Hagelskjaer Kristensen, Marianne Voldstedlund, Jørgen Prag
Fusobacterium necrophorum findings in Denmark and estimation of the incidence of F. necrophorum bacteraemia was described using data from the nationwide Danish microbiology database (MiBa). All microbiological reports on any Fusobacterium species in Denmark were extracted for a period of 5 years from 2010 to 2014 from MiBa and from the local department of clinical microbiology. The overall incidence of F. necrophorum bacteraemia from 2010 to 2014 was 2.8 cases per million/year vs 9.4 in the age group 15-24 years...
October 5, 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Lin Wang, Xianju Xie, Michael D Weir, Ashraf F Fouad, Liang Zhao, Hockin H K Xu
The objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. Bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. Nanoparticles of amorphous calcium phosphate at 30 wt% was mixed into adhesive...
November 2016: Journal of Materials Science. Materials in Medicine
Jessie Qy Liang, Jonathan Chiu, Yingxuan Chen, Yanqin Huang, Akira Higashimori, Jing-Yuan Fang, Hassan Brim, Hassan Ashktorab, Siew Chien Ng, Simon Sm Ng, Shu Zheng, Francis Kl Chan, Joseph Jy Sung, Jun Yu
PURPOSE: Gut microbiota have been implicated in the development of colorectal cancer (CRC). We evaluated the utility of fecal bacterial marker candidates identified by our metagenome sequencing analysis for CRC diagnosis. EXPERIMENTAL DESIGN: 439 subjects (203 CRC and 236 healthy subjects) from two independent Asian cohorts were included. Probe-based duplex quantitative PCR (qPCR) assays were established for quantification of bacterial marker candidates. RESULTS: Candidates identified by metagenome sequencing, including Fusobacterium nucleatum (Fn), Bacteroides clarus (Bc), Roseburia intestinalis (Ri), Clostridium hathewayi (Ch), and one undefined species (labeled as m7), were examined in fecal samples of 203 CRC patients and 236 healthy controls by duplex-qPCR...
October 3, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
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