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https://www.readbyqxmd.com/read/27863261/a-next-generation-sequencing-strategy-for-evaluating-the-most-common-genetic-abnormalities-in-multiple-myeloma
#1
Cristina Jiménez, María Jara-Acevedo, Luis A Corchete, David Castillo, Gonzalo R Ordóñez, M Eugenia Sarasquete, Noemí Puig, Joaquín Martínez-López, M Isabel Prieto-Conde, María García-Álvarez, M Carmen Chillón, Ana Balanzategui, Miguel Alcoceba, Albert Oriol, Laura Rosiñol, Luis Palomera, Ana I Teruel, Juan J Lahuerta, Joan Bladé, María V Mateos, Alberto Orfão, Jesús F San Miguel, Marcos González, Norma C Gutiérrez, Ramón García-Sanz
Identification and characterization of genetic alterations are essential for diagnosis of multiple myeloma and may guide therapeutic decisions. Currently, genomic analysis of myeloma to cover the diverse range of alterations with prognostic impact requires fluorescence in situ hybridization (FISH), single nucleotide polymorphism arrays, and sequencing techniques, which are costly and labor intensive and require large numbers of plasma cells. To overcome these limitations, we designed a targeted-capture next-generation sequencing approach for one-step identification of IGH translocations, V(D)J clonal rearrangements, the IgH isotype, and somatic mutations to rapidly identify risk groups and specific targetable molecular lesions...
November 15, 2016: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/27858228/prenatal-diagnosis-of-abnormal-umbilical-cord-insertion-a-rare-case-of-furcate-insertion
#2
Yasuyuki Fujita, Yasuo Yumoto, Kiyoko Kato
Furcate insertion (FI) is an extremely rare abnormality of umbilical cord insertion. One of the complications associated with FI is hemorrhage from the umbilical vein at the site of FI of the umbilical cord, which can cause sudden intrauterine fetal death. Because of its rarity, no prenatal diagnosis of FI has been reported. A 31-year-old woman at 34 weeks' gestation was referred to us for suspected abnormal cord insertion. Ultrasonography showed normal fetal growth and amniotic fluid volume, with no fetal anomalies...
November 17, 2016: Journal of Medical Ultrasonics
https://www.readbyqxmd.com/read/27855185/aberrant-expression-of-timp-2-and-pbef-genes-in-the-placentae-of-cloned-mice-due-to-epigenetic-reprogramming-error
#3
Hong Rye Kim, Jae Eun Lee, Reza Kheirkhahi Oqani, So Yeon Kim, Teruhiko Wakayama, Chong Li, Su Jin Sa, Je Seok Woo, Dong Il Jin
Cloned mice derived from somatic or ES cells show placental overgrowth (placentomegaly) at term. We had previously analyzed cloned and normal mouse placentae by using two-dimensional gel electrophoresis and mass spectrometry to identify differential protein expression patterns. Cloned placentae showed upregulation of tissue inhibitor of metalloproteinase-2 (TIMP-2), which is involved in extracellular matrix degradation and tissue remodeling, and downregulation of pre-B cell colony enhancing factor 1 (PBEF), which inhibits apoptosis and induces spontaneous labor...
2016: PloS One
https://www.readbyqxmd.com/read/27829301/historical-state-of-knowledge-of-the-health-risks-of-asbestos-posed-to-seamen-on-merchant-ships
#4
David G Dodge, Barbara D Beck
We examined the development of knowledge concerning the risks posed by asbestos to seamen working aboard merchant ships at sea (i.e. commercial, rather than naval vessels). Seamen were potentially exposed to "in-place" asbestos on merchant ships by performing intermittent repair and maintenance tasks. We reviewed studies measuring airborne asbestos onboard merchant ships and health outcomes of merchant seamen, as well as studies, communications, and actions of U.S. organizations with roles in maritime health and safety...
November 10, 2016: Inhalation Toxicology
https://www.readbyqxmd.com/read/27824742/hip-dislocation-and-dystocia-in-early-medieval-times-possible-evidence-of-labor-maneuver
#5
Assumpció Malgosa, Susana Carrascal, Giampaolo Piga, Albert Isidro
BACKGROUND: In ancient times, maternal mortality would occur frequently, particularly during labor. Evidence of dystocia resulting in the death of a pregnant woman is very infrequent in paleopathologic literature, with only a few cases being demonstrated. CASE: In the early medieval site of Casserres, the skeleton of a young woman with a fetus in the pelvic region was found. Some abnormal findings of the maternal skeleton were evaluated, including a sacral anomaly, femoral head wound, the rare position of the lower left limb with the femoral head dislodged anteriorly and cephalad from the socket, and a fibular fracture...
December 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27799572/utility-of-multiparametric-mri-in-caesarean-section-scar-characterization-and-preoperative-prediction-of-scar-dehiscence-a-prospective-study
#6
Ishan Kumar, Ashish Verma, Manjari Matah, Gayatri Satpathy
BACKGROUND: Post-Caesarean uterine scar rupture during vaginal birth after Caesarean section (VBAC) is a potentially life-threatening complication. Prediction of scar dehiscence and scar rupture is vital in treatment planning and selecting candidates of trial of labor after a Caesarean section (CS). PURPOSE: To assess the accuracy of magnetic resonance imaging (MRI) for evaluation of post-Caesarean uterine scar and to predict scar dehiscence during repeat CS. MATERIAL AND METHODS: Thirty patients with a history of at least one previous CS underwent pelvic MRI for assessment of uterine scar during a subsequent gestation, all of whom underwent lower segment Caesarean section (LSCS) subsequently due to one of the established indications of CSs...
October 31, 2016: Acta Radiologica
https://www.readbyqxmd.com/read/27798550/bone-age-and-mineral-density-assessments-using-plain-roentgenograms-in-tenofovir-exposed-infants-in-malawi-and-brazil-enrolled-in-hiv-prevention-trials-network-hptn-057
#7
Luiz Eduardo Osorio, Maria Ines Boechat, Mark Mirochnick, Newton Kumwenda, Regis Kreitchmann, Lynda Emel, Jorge Pinto, Esau Joao, Breno Santos, Molly Swenson, Kathleen George, Paul Sato, Lynne Mofenson, Karin Nielsen-Saines
BACKGROUND: Tenofovir disoproxil fumarate (TDF) use during pregnancy has been increasing and studies linking bone toxicity with exposure to TDF have raised concern for its use in infants. METHODS: Hand/wrist and spine radiographs were obtained at 3 days and 12 weeks of age in infants born to HIV-infected pregnant women enrolled in the HIV Prevention Trials Network (HPTN) 057 pharmacokinetic study of TDF conducted in Malawi and Brazil assigned to three TDF dosing cohorts...
October 27, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27776845/-the-use-of-progestatives-for-the-prevention-of-spontaneous-preterm-birth
#8
E Azria
OBJECTIVES: To identify clinical situations in which progestatives used to reduce the risk of spontaneous preterm delivery and/or reduced adverse neonatal outcomes have been evaluated and identify situations in which 17OHPC or vaginal progesterone might be recommended. METHODS: Bibliographic searches were performed in the Medline and Cochrane databases with the use of a combination of keywords and text words related to "progesterone", "tocolysis", and "preterm labor" from 1956 through July 2016...
October 21, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27776071/practice-bulletin-no-173-fetal-macrosomia
#9
(no author information available yet)
Suspected fetal macrosomia is encountered commonly in obstetric practice. As birth weight increases, the likelihood of labor abnormalities, shoulder dystocia, birth trauma, and permanent injury to the neonate increases. The purpose of this document is to quantify those risks, address the accuracy and limitations of methods for estimating fetal weight, and suggest clinical management for a pregnancy with suspected fetal macrosomia.
November 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27776066/practice-bulletin-no-173-summary-fetal-macrosomia
#10
(no author information available yet)
Suspected fetal macrosomia is encountered commonly in obstetric practice. As birth weight increases, the likelihood of labor abnormalities, shoulder dystocia, birth trauma, and permanent injury to the neonate increases. The purpose of this document is to quantify those risks, address the accuracy and limitations of methods for estimating fetal weight, and suggest clinical management for a pregnancy with suspected fetal macrosomia.
November 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27766977/factors-associated-with-the-use-of-supplemental-oxygen-or-positive-pressure-ventilation-in-the-delivery-room-in-infants-born-with-a-gestational-age%C3%A2-%C3%A2-%C3%A2-34%C3%A2-weeks
#11
Maria Elisabeth Moreira, Ana Paula Esteves Pereira, Saint Clair Gomes Junior, Ruth Guinsburg, Maria Fernanda Branco de Almeida, Silvana Granado Gama, Maria do Carmo Leal
BACKGROUND: Approximately 5-10 % of newborns require some form of resuscitationupon delivery; several factors, such as maternal abnormal conditions, gestational age and type of delivery could be responsible for this trend. This study aimed to describe the factors associated with the need for positive pressure ventilation (PPV) via a mask or endotracheal tube and the use of supplemental O2 in newborns with a gestational age greater than 34 weeks in Brazil. METHODS: We performed a cross-sectional study and obtained data from the Birth in Brazil Survey...
October 17, 2016: Reproductive Health
https://www.readbyqxmd.com/read/27755062/amniotic-fluid-embolism
#12
Courtney Stanley Sundin, Lauren Bradham Mazac
Amniotic fluid embolism (AFE) is a rare but serious and potentially deadly complication of pregnancy that is unpreventable and unpredictable. Most AFE events occur during labor; however, approximately one third happen during the immediate postpartum period. Presentation is abrupt and thought to be an abnormal response to fetal materials entering maternal circulation through the placental insertion site. Care providers must recognize the signs and symptoms of AFE and react quickly in effort to treat potential complications...
October 13, 2016: MCN. the American Journal of Maternal Child Nursing
https://www.readbyqxmd.com/read/27716543/deficiency-in-memory-b-cell-compartment-in-a-patient-with-infertility-and-recurrent-pregnancy-losses
#13
N Sung, H J Byeon, M D Salazar Garcia, A Skariah, L Wu, S Dambaeva, K Beaman, A Gilman-Sachs, J Kwak-Kim
Alterations in normal balance of B cell subsets have been reported in various rheumatic diseases. In this study, we report a woman with a history of recurrent pregnancy losses (RPL) and infertility who had low levels of memory B cells. A 35-year-old woman with a history of RPL and infertility was demonstrated to have increased peripheral blood CD19+ B cells with persistently low levels of memory B cell subsets. Prior to the frozen donor egg transfer cycle, prednisone and intravenous immunoglobulin G (IVIg) treatment was initiated and patient achieved dichorionic diamniotic twin pregnancies...
September 28, 2016: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/27701372/may-pre-procedure-neuroaxial-ultrasound-improve-the-identification-of-potential-epidural-space-when-compared-with-anatomical-landmarks-a-prospective-randomized-study
#14
Paolo Perna, Antonio Gioia, Riccardo Ragazzi, Carlo A Volta, Massimo Innamorato
BACKGROUND: Regional epidural analgesia is considered the gold standard for pain treatment in labour but the epidural catheter placement may be a difficult procedure because of the difficulty in anatomical landmarks palpation. These difficulties increase in pregnant women. Pre-procedure neuroaxial ultrasound may facilitate the procedure. METHODS: Prospectic randomized controlled study conducted in the Labour Ward. We randomized two groups of women undergoing epidural analgesia: Group A (28 patients), with loss of resistance technique and Group B (30 patients) with US assisted procedure...
October 4, 2016: Minerva Anestesiologica
https://www.readbyqxmd.com/read/27670710/management-of-spontaneous-labour-at-term%C3%A2-in-healthy-women
#15
Lily Lee, Jessica Dy, Hussam Azzam
OBJECTIVE: The purpose of this guideline is to provide guidance for the intrapartum management of spontaneous labour, whether normal or abnormal, in term, healthy women, and to provide guidance in the management of first and second stage dystocia to increase the likelihood of a vaginal birth and optimize birth outcomes. EVIDENCE: Published literature was retrieved through searches of PubMed and the Cochrane Library in October 2011 using appropriate, controlled vocabulary (e...
September 2016: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/27666886/-prenatal-ultrasound-diagnosis-prognosis-and-chromosome-analysis-of-fetal-bowel-dilatation
#16
R Q Yang, Z Y Liu, J L Zhang, Y Nan, L M Fan
Objective: To investigate the relationship among the prenatal ultrasound, prognosis and chromosome results of fetal bowel dilatation. And in order to further improve the positive rate of prenatal chromosome examination operation, and reduce the risk of missed diagnosis. Methods: Ninety fetuses who were diagnosed as fetal bowel dilatation by prenatal ultrasound from June 2013 to December 2015 in the Second hospital of Jilin University were enrolled. Chromosomes were examined by amniotic cavity puncture or umbilical cord blood puncture...
September 6, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27651618/indications-for-cesarean-sections-in-rural-nepal
#17
Live Johanne Samdal, Kristina Reberg Steinsvik, Prakash Pun, Phanindra Dani, Borghild Roald, Babill Stray-Pedersen, Erik Bøhler
OBJECTIVE: Globally, cesarean section (CS) rate is close to 26 %. Nepal has a reported CS rate of 5 %, with huge differences in rural (3.5 %) and urban (15 %) areas. The aim of the study was to determine the rate and indications for CS in a remote hospital in a rural area of Nepal. METHODS: A one-year cross-sectional prospective study from August 2014 to August 2015 was performed at Okhaldhunga Community Hospital (OCH). Semi-structured interviews of all women undergoing CS (91) were done, partly with the assistance of a local translator...
October 2016: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/27651575/obstetric-outcome-during-an-era-of-active-management-for-obstetrics-cholestasis
#18
Nalini Sharma, S Panda, Ahanthem Santa Singh
OBJECTIVE: To study the incidence, maternal, and perinatal outcomes in intrahepatic cholestasis of pregnancy with active management. MATERIALS AND METHODS: This observational study included 48 cases diagnosed as intrahepatic cholestasis of pregnancy. Medical treatment and active management (fetal surveillance and termination of pregnancy at 37-38 weeks) were offered to all. Maternal and perinatal outcomes are studied. RESULTS: Incidence of ICP was 2...
October 2016: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/27644455/novel-rapid-molecular-diagnosis-of-fetal-chromosomal-abnormalities-associated-with-recurrent-pregnancy-loss
#19
Lan Yang, Ye Tang, Mudan Lu, Yuefen Yang, Jianpin Xiao, Qiaoxia Wang, Canfeng Yang, Hehua Tao, Xiangjing Ying
INTRODUCTION: Labor-intensive karyotyping is used as the gold standard diagnostic test to identify copy number variants (CNVs) in the fetal genome after recurrent pregnancy loss. Our aim was to present and evaluate a novel molecular assay called CNVplex that could potentially be used as an alternate method to conventional karyotyping for diagnosing fetal chromosomal abnormalities associated with recurrent pregnancy loss. MATERIAL AND METHODS: Using karyotyping as the reference standard, CNVplex was performed to identify fetal chromosomal abnormalities in the chorionic villus samples from 76 patients experiencing at least two pregnancy losses...
September 19, 2016: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/27642489/neonatal-respiratory-distress-in-a-reference-neonatal-unit-in-cameroon-an-analysis-of-prevalence-predictors-etiologies-and-outcomes
#20
Joel Noutakdie Tochie, Simeon-Pierre Choukem, Regina Ndasi Langmia, Esther Barla, Paul Koki-Ndombo
INTRODUCTION: Neonatal respiratory distress (NRD) is a main cause of neonatal morbidity and mortality in developing countries. Early detection of its risk factors and early treatment of its etiologies are major challenges. However, few studies in developing countries have provided data needed to tackle it. We aimed to determine the prevalence, predictors, etiologies and outcome of NRD in a tertiary health care centre of Cameroon. METHODS: We analyzed the hospital files of all newborns admitted to the Neonatal unit of Douala General Hospital from 1(st) January 2011 to 28(th) February 2013...
2016: Pan African Medical Journal
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