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https://www.readbyqxmd.com/read/28133905/simultaneous-detection-of-45-fusion-genes-in-leukemia-by-dual-color-fluorescence-real-time-pcr
#1
Z Zheng, P Zhang, G He, K Liao, Z Wang, J Pan, K Du, J Du, B-A Li
INTRODUCTION: Detection of recurrent genetic abnormalities is of great significance for a refined diagnosis and assessment of prognosis in leukemia. Conventional nested reverse transcription PCR is labor intensive and time-consuming. METHODS: We have developed a novel dual-color TaqMan probe-based real-time PCR method for the simultaneous screening of 45 fusion transcripts in 12 parallel reactions. The method was tested and validated with cell lines carrying known fusion transcripts and patient samples...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28131122/obstetric-and-anesthetic-approaches-to-external-cephalic-version
#2
REVIEW
Stephanie Lim, Jennifer Lucero
Breech presentation is the most common abnormal fetal presentation and complicates approximately 3% to 4% of all pregnancies. External cephalic version (ECV) should be recommended to women with a breech singleton pregnancy, if there is no maternal or fetal contraindication. ECV increases the chance of cephalic presentation at the onset of labor and decreases the rate of cesarean delivery by almost 40%. The success rate of ECV is approximately 60%. Review of the risks and benefits for performing an ECV and for both the timing of ECV and the number of attempts should be should be discussed with the patient...
March 2017: Anesthesiology Clinics
https://www.readbyqxmd.com/read/28098674/diethylstilbestrol-potential-health-risks-for-women-exposed-in-utero-and-their-offspring
#3
J Leocadia Conlon
Diethylstilbestrol (DES) is a synthetic estrogen given to pregnant women to prevent miscarriages and preterm labor; the drug was used between 1941 and 1971 in the United States and into the 1980s in other countries. DES exposure is associated with significant long-term health effects, including increased risk for breast cancer, cervical and vaginal clear cell adenocarcinoma, reproductive tract abnormalities, infertility, poor pregnancy outcomes, and early menopause. This article reviews the potential health risks associated with DES exposure, how to assess which patients are at risk, and management recommendations for patients exposed to DES...
February 2017: JAAPA: Official Journal of the American Academy of Physician Assistants
https://www.readbyqxmd.com/read/28061767/the-effect-of-induction-method-in-twin-pregnancies-a-secondary-analysis-for-the-twin-birth-study
#4
Elad Mei-Dan, Elizabeth V Asztalos, Andrew R Willan, Jon F R Barrett
BACKGROUND: This secondary analysis for the Twin Birth Study, an international, multicenter trial, aimed to compare the cesarean section rates and safety between methods of induction of labor in twin pregnancies. METHODS: Women with twin pregnancies where the first twin was in a cephalic presentation and who presented for labor induction, were non-randomly assigned to receive prostaglandin or amniotomy and/or oxytocin. Main outcome measures were the rates of unplanned cesarean section and neonatal and maternal mortality or serious morbidity...
January 6, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28034657/failure-of-physiologic-transformation-of-spiral-arteries-endothelial-and-trophoblast-cell-activation-and-acute-atherosis-in-the-basal-plate-of-the-placenta
#5
Carlos A Labarrere, Hector L Dicarlo, Elaine Bammerlin, James W Hardin, Yeon M Kim, Piya Chaemsaithong, David M Haas, Ghassan S Kassab, Roberto Romero
BACKGROUND: Failure of physiologic transformation of spiral arteries has been reported in preeclampsia, fetal growth restriction, fetal death, and spontaneous preterm labor with intact or ruptured membranes. Spiral arteries with failure of physiologic transformation are prone to develop atherosclerotic-like lesions of atherosis. There are striking parallels between preeclampsia and atherosclerotic disease, and between lesions of atherosis and atherosclerosis. Endothelial activation, identified by intercellular adhesion molecule-1 (ICAM-1) expression, is present in atherosclerotic-like lesions of heart transplantation, and is considered a manifestation of rejection...
December 26, 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28009308/-twin-to-twin-transfusion-syndrome-the-possibility-of-latent-flow-case-report
#6
E Kamyshanskiy, O Kostyleva, S Musabekova, M Tussupbekova, I Kopobaeva
The early diagnosis of the Twin-To-Twin transfusion syndrome (TTTS) carries benefits of the better survivor for the fetus-donor; but at the same time there are obvious practical impediments to diagnose the condition earlier in the course of a pregnancy. That's why this particular problem in practical medicine requires in depth investigation to clarify casual factors, and specify tactical approach to the prospective patients which in turn will lead to prevention of the fetal death and damage and improvement of fetal survivor in any form of TTTS...
November 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27984035/trends-in-gestational-age-at-time-of-surgical-abortion-for-fetal-aneuploidy-and-structural-abnormalities
#7
Anne R Davis, Sarah K Horvath, Paula M Castaño
BACKGROUND: Screening for fetal aneuploidy has evolved over the past two decades. Whether these advances impact gestational age at abortion has received little study. OBJECTIVE: To describe trends in the gestational age at the time of abortion by fetal diagnosis over an eleven-year study period. We hypothesized that gestational age at time of abortion would decrease for fetal aneuploidy but remain unchanged for structural abnormalities. STUDY DESIGN: We conducted a retrospective case series of all women undergoing surgical abortion for fetal aneuploidy or structural abnormalities up to 24 weeks gestation from 2004 through 2014 in a hospital operating room setting at a single, urban medical center...
October 27, 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27960173/fetal-heart-rate-variability-validation-of-a-new-continuous-noninvasive-computerized-analysis
#8
Charles Garabedian, Laura Butruille, Elie Servan-Schreiber, Grégoire Ficheur, Laurent Storme, Philippe Deruelle, Julien De Jonckheere, Véronique Houfflin-Debarge
OBJECTIVE: We developed a computerized heart-rate variability index related to the fetal parasympathetic activity: the Fetal Stress Index (FSI). The objective was to determine whether the FSI is related to the visual analysis of the fetal heart rate (FHR). METHODS: Thirty tracings recorded at a labor ward were classified according to the NICHD categories: (I) normal FHR tracing, (II) intermediate risk of acidosis, and (III) high risk. FSI was calculated as minimum, maximum, and mean, and was evaluated before the onset of the FHR pattern, during the 10 min following, and between 10 and 20 min after that...
December 14, 2016: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/27957373/a-rare-case-report-of-thoracic-ectopia-cordis-an-obstetrician-s-point-of-view-in-multidisciplinary-approach
#9
Diana Ramasauskaite, Vilija Snieckuviene, Viktorija Zitkute, Ramune Vankeviciene, Dalia Lauzikiene, Grazina Drasutiene
Ectopia cordis is a rare congenital anomaly associated with the heart positioned outside of the thoracic cavity either partially or completely. It can be associated with other congenital abnormalities. Overall, the prognosis for infants with ectopia cordis is very poor but depends greatly on the type and severity of ectopia cordis and intracardiac and associated malformations. We present one case of a fetus with prenatally diagnosed thoracic ectopia cordis with intracardiac defects and omphalocele, all the abnormalities seen in pentalogy of Cantrell except a diaphragmatic defect...
2016: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/27956203/syphilis-during-pregnancy-a-preventable-threat-to-maternal-fetal-health
#10
REVIEW
Martha W F Rac, Paula A Revell, Catherine S Eppes
Syphilis remains the most common congenital infection worldwide and has tremendous consequences for the mother and her developing fetus if left untreated. Recently, there has been an increase in the number of congenital syphilis cases in the United States. Thus, recognition and appropriate treatment of reproductive-age women must be a priority. Testing should be performed at initiation of prenatal care and twice during the third trimester in high-risk patients. There are 2 diagnostic algorithms available and physicians should be aware of which algorithm is utilized by their testing laboratory...
December 9, 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27936988/effect-of-fetal-gender-on-induction-of-labor-failure-rates
#11
Eran Ashwal, Eran Hadar, Rony Chen, Amir Aviram, Liran Hiersch, Rinat Gabbay-Benziv
PURPOSE: To evaluate gender effect on induction of labor (IoL) failure rates stratified by indication to delivery. METHODS: Retrospective cohort analysis of singleton pregnancies 34-42 weeks undergoing cervical ripening using controlled-release PGE2 vaginal insert. IoL Indications were divided to: (1) maternal; (2) hypertensive disorders; (3) premature rupture of membrane or (4) fetal (growth abnormalities, oligohydramnios, postdate etc). IoL failure was defined as: (1) Bishop-score ≤7 24 hours after PGE2; (2) cesarean delivery due to failed induction; (3) fetal distress followed by PGE2 removal and emergent cesarean...
December 11, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27863261/a-next-generation-sequencing-strategy-for-evaluating-the-most-common-genetic-abnormalities-in-multiple-myeloma
#12
Cristina Jiménez, María Jara-Acevedo, Luis A Corchete, David Castillo, Gonzalo R Ordóñez, María E Sarasquete, Noemí Puig, Joaquín Martínez-López, María I Prieto-Conde, María García-Álvarez, María C Chillón, Ana Balanzategui, Miguel Alcoceba, Albert Oriol, Laura Rosiñol, Luis Palomera, Ana I Teruel, Juan J Lahuerta, Joan Bladé, María V Mateos, Alberto Orfão, Jesús F San Miguel, Marcos González, Norma C Gutiérrez, Ramón García-Sanz
Identification and characterization of genetic alterations are essential for diagnosis of multiple myeloma and may guide therapeutic decisions. Currently, genomic analysis of myeloma to cover the diverse range of alterations with prognostic impact requires fluorescence in situ hybridization (FISH), single nucleotide polymorphism arrays, and sequencing techniques, which are costly and labor intensive and require large numbers of plasma cells. To overcome these limitations, we designed a targeted-capture next-generation sequencing approach for one-step identification of IGH translocations, V(D)J clonal rearrangements, the IgH isotype, and somatic mutations to rapidly identify risk groups and specific targetable molecular lesions...
January 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/27858228/prenatal-diagnosis-of-abnormal-umbilical-cord-insertion-a-rare-case-of-furcate-insertion
#13
Yasuyuki Fujita, Yasuo Yumoto, Kiyoko Kato
Furcate insertion (FI) is an extremely rare abnormality of umbilical cord insertion. One of the complications associated with FI is hemorrhage from the umbilical vein at the site of FI of the umbilical cord, which can cause sudden intrauterine fetal death. Because of its rarity, no prenatal diagnosis of FI has been reported. A 31-year-old woman at 34 weeks' gestation was referred to us for suspected abnormal cord insertion. Ultrasonography showed normal fetal growth and amniotic fluid volume, with no fetal anomalies...
November 17, 2016: Journal of Medical Ultrasonics
https://www.readbyqxmd.com/read/27855185/aberrant-expression-of-timp-2-and-pbef-genes-in-the-placentae-of-cloned-mice-due-to-epigenetic-reprogramming-error
#14
Hong Rye Kim, Jae Eun Lee, Reza Kheirkhahi Oqani, So Yeon Kim, Teruhiko Wakayama, Chong Li, Su Jin Sa, Je Seok Woo, Dong Il Jin
Cloned mice derived from somatic or ES cells show placental overgrowth (placentomegaly) at term. We had previously analyzed cloned and normal mouse placentae by using two-dimensional gel electrophoresis and mass spectrometry to identify differential protein expression patterns. Cloned placentae showed upregulation of tissue inhibitor of metalloproteinase-2 (TIMP-2), which is involved in extracellular matrix degradation and tissue remodeling, and downregulation of pre-B cell colony enhancing factor 1 (PBEF), which inhibits apoptosis and induces spontaneous labor...
2016: PloS One
https://www.readbyqxmd.com/read/27829301/historical-state-of-knowledge-of-the-health-risks-of-asbestos-posed-to-seamen-on-merchant-ships
#15
David G Dodge, Barbara D Beck
We examined the development of knowledge concerning the risks posed by asbestos to seamen working aboard merchant ships at sea (i.e. commercial, rather than naval vessels). Seamen were potentially exposed to "in-place" asbestos on merchant ships by performing intermittent repair and maintenance tasks. We reviewed studies measuring airborne asbestos onboard merchant ships and health outcomes of merchant seamen, as well as studies, communications, and actions of U.S. organizations with roles in maritime health and safety...
December 2016: Inhalation Toxicology
https://www.readbyqxmd.com/read/27824742/hip-dislocation-and-dystocia-in-early-medieval-times-possible-evidence-of-labor-maneuver
#16
Assumpció Malgosa, Susana Carrascal, Giampaolo Piga, Albert Isidro
BACKGROUND: In ancient times, maternal mortality would occur frequently, particularly during labor. Evidence of dystocia resulting in the death of a pregnant woman is very infrequent in paleopathologic literature, with only a few cases being demonstrated. CASE: In the early medieval site of Casserres, the skeleton of a young woman with a fetus in the pelvic region was found. Some abnormal findings of the maternal skeleton were evaluated, including a sacral anomaly, femoral head wound, the rare position of the lower left limb with the femoral head dislodged anteriorly and cephalad from the socket, and a fibular fracture...
December 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27799572/utility-of-multiparametric-mri-in-caesarean-section-scar-characterization-and-preoperative-prediction-of-scar-dehiscence-a-prospective-study
#17
Ishan Kumar, Ashish Verma, Manjari Matah, Gayatri Satpathy
BACKGROUND: Post-Caesarean uterine scar rupture during vaginal birth after Caesarean section (VBAC) is a potentially life-threatening complication. Prediction of scar dehiscence and scar rupture is vital in treatment planning and selecting candidates of trial of labor after a Caesarean section (CS). PURPOSE: To assess the accuracy of magnetic resonance imaging (MRI) for evaluation of post-Caesarean uterine scar and to predict scar dehiscence during repeat CS. MATERIAL AND METHODS: Thirty patients with a history of at least one previous CS underwent pelvic MRI for assessment of uterine scar during a subsequent gestation, all of whom underwent lower segment Caesarean section (LSCS) subsequently due to one of the established indications of CSs...
October 31, 2016: Acta Radiologica
https://www.readbyqxmd.com/read/27798550/bone-age-and-mineral-density-assessments-using-plain-roentgenograms-in-tenofovir-exposed-infants-in-malawi-and-brazil-enrolled-in-hiv-prevention-trials-network-057
#18
Luiz Eduardo Osorio, Maria Ines Boechat, Mark Mirochnick, Newton Kumwenda, Regis Kreitchmann, Lynda Emel, Jorge Pinto, Esau Joao, Breno Santos, Molly Swenson, Kathleen George, Paul Sato, Lynne Mofenson, Karin Nielsen-Saines
BACKGROUND: Tenofovir disoproxil fumarate (TDF) use during pregnancy has been increasing, and studies linking bone toxicity with exposure to TDF have raised concern for its use in infants. METHODS: Hand/wrist and spine radiographs were obtained at 3 days and 12 weeks of age in infants born to HIV-infected pregnant women enrolled in the HIV Prevention Trials Network 057 pharmacokinetic study of TDF conducted in Malawi and Brazil assigned to 3 TDF dosing cohorts. In cohort 1, mothers received 600 mg of TDF during labor...
February 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27776845/-the-use-of-progestatives-for-the-prevention-of-spontaneous-preterm-birth
#19
E Azria
OBJECTIVES: To identify clinical situations in which progestatives used to reduce the risk of spontaneous preterm delivery and/or reduced adverse neonatal outcomes have been evaluated and identify situations in which 17OHPC or vaginal progesterone might be recommended. METHODS: Bibliographic searches were performed in the Medline and Cochrane databases with the use of a combination of keywords and text words related to "progesterone", "tocolysis", and "preterm labor" from 1956 through July 2016...
December 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27776071/practice-bulletin-no-173-fetal-macrosomia
#20
(no author information available yet)
Suspected fetal macrosomia is encountered commonly in obstetric practice. As birth weight increases, the likelihood of labor abnormalities, shoulder dystocia, birth trauma, and permanent injury to the neonate increases. The purpose of this document is to quantify those risks, address the accuracy and limitations of methods for estimating fetal weight, and suggest clinical management for a pregnancy with suspected fetal macrosomia.
2016: Obstetrics and Gynecology
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