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https://www.readbyqxmd.com/read/28239394/standardization-and-validation-of-dot-elisa-assay-for-paracoccidioides-brasiliensis-antibody-detection
#1
Camila Mika Kamikawa, Rinaldo Poncio Mendes, Adriana Pardini Vicentini
BACKGROUND: Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28239052/an-atypical-case-of-recurrent-carotid-body-carcinoma-in-a-young-adult-dog-histopathological-immunohistochemical-and-electron-microscopic-study
#2
Angeline Ping Ping Teh, Watanyoo Pratakpiriya, Yuichi Hidaka, Hiroyuki Sato, Takuya Hirai, Ryoji Yamaguchi
A 3.5-year-old female Chihuahua was presented with complaint of neck pain, intermittent cough and dysphagia. Physical examination and diagnostic imaging of neck region revealed a solid and highly vascularized mass involving the retropharyngeal region. Histologically, the mass showed an atypical zellballen pattern which comprised of high density of type I chief cells with high nuclear cytoplasmic ratio and separated by delicate fibrovascular stroma. Immunoreactivity for neuroendocrine markers was diffusely positive in cytoplasm of tumor cells...
February 27, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28238969/biocompatibility-properties-of-polyamide-6-pcl-blends-composite-textile-scaffold-using-ea-hy926-human-endothelial-cells
#3
Abdalla Abdal-Hay, Khalil Abdelrazek Khalil, Fawzi F Al-Jassir, Amira Gamal-Eldeen
Enhancing the cytocompatibility profiles including cell attachment, growth and viability of designed synthetic scaffolds have a pivotal role in tissue engineering applications. Polymer blending is one of the most effective methods for providing new desirable biomaterials for tissue scaffolds. This article reports a novel polyamide 6/ poly(Ɛ-caprolactone) (PA6/PCL) blends solution by varying the concentrations ratios of PA6 and PCL which was fabricated to create composite fibrous tissue scaffolds. Highly porous blends fibrous scaffold has been fabricated and their suitability as cell-support for EA...
February 27, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28231293/photoacoustic-imaging-features-of-intraocular-tumors-retinoblastoma-and-uveal-melanoma
#4
Guan Xu, Yafang Xue, Zeynep Gürsel Özkurt, Naziha Slimani, Zizhong Hu, Xueding Wang, Kewen Xia, Teng Ma, Qifa Zhou, Hakan Demirci
The purpose of this study is to examine the capability of photoacoustic (PA) imaging (PAI) in assessing the unique molecular and architectural features in ocular tumors. A real-time PA and ultrasonography (US) parallel imaging system based on a research US platform was developed to examine retinoblastoma in mice in vivo and human retinoblastoma and uveal melanoma ex vivo. PA signals were generated by optical illumination at 720, 750, 800, 850, 900 and 950 nm delivered through a fiber optical bundle. The optical absorption spectra of the tumors were derived from the PA images...
2017: PloS One
https://www.readbyqxmd.com/read/28228021/waiting-time-distribution-for-electron-transport-in-a-molecular-junction-with-electron-vibration-interaction
#5
Daniel S Kosov
On the elementary level, electronic current consists of individual electron tunnelling events that are separated by random time intervals. The waiting time distribution is a probability to observe the electron transfer in the detector electrode at time t+τ given that an electron was detected in the same electrode at an earlier time t. We study waiting time distribution for quantum transport in a vibrating molecular junction. By treating the electron-vibration interaction exactly and molecule-electrode coupling perturbatively, we obtain the master equation and compute the distribution of waiting times for electron transport...
February 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28227655/development-of-a-snake-like-dexterous-manipulator-for-skull-base-surgery
#6
Suat Coemert, Anzhu Gao, John P Carey, Mattias F Traeger, Russell H Taylor, Tim C Lueth, Mehran Armand, Suat Coemert, Anzhu Gao, John P Carey, Mattias F Traeger, Russell H Taylor, Tim C Lueth, Mehran Armand, Anzhu Gao, Russell H Taylor, Mattias F Traeger, Mehran Armand, Suat Coemert, Tim C Lueth, John P Carey
Petrous apex lesions constitute considerable surgical challenges due to their location in the skull base and close relationship with critical structures such as inner ear, carotid arteries, facial nerves and jugular bulb. These lesions often cannot be treated completely with rigid tools due to the limited accessibility. We are aiming to develop a snake-like manipulator to assist surgeons with the infralabyrinthine treatment of petrous apex lesions with increased dexterity. This snake-like dexterous manipulator (SDM) with 3...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28224975/intraperitoneal-infusion-of-neutral-ph-superoxidized-solution-in-rats-evaluation-of-toxicity-and-complications-on-peritoneal-surface-and-liver
#7
Abbas Aras, Erbil Karaman, Serkan Yıldırım, Özkan Yılmaz, Remzi Kızıltan, Kamuran Karaman
BACKGROUND Superoxidized water (SOW) is known to be a potent disinfectant. The aim of this study was to evaluate the toxicity and complications on the peritoneal surface and liver after infusion of pH-neutral SOW into the peritoneal cavity of rats. MATERIAL AND METHODS Thirty Wistar-Albino rats weighing 250-300 g were randomly divided into 3 groups (10 rats/group). Group1 (control group) rats received single dose of 10 mg/kg saline solution intraperitoneally. Group 2 (single-dose group) rats received a single dose of 10 mg/kg pH-neutral SOW intraperitoneally...
February 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28224033/distribution-and-risk-factors-associated-with-intestinal-parasite-infections-among-children-with-gastrointestinal-disorders
#8
Hamed Kiani, Ali Haghighi, Roya Salehi, Eznollah Azargashb
AIM: Prevalence and risk factors associated with intestinal parasites among children ≤ 12 years old in Nahavand county western Iran, was the objective of this search. BACKGROUND: Intestinal parasites (IPs) are important health problems among most societies. METHODS: This cross sectional study was carried out during 6 months from April to September 2014 in Nahavand County western Iran. Fecal samples were collected from 500 children suffering from gastrointestinal disorders (GIDs) and examined by macroscopy and microscopic (using saline and iodine wet mount, formalin-ether sedimentation, Trichrome and modified Ziehl Neelsen staining) methods...
December 2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28223223/berberine-improves-cognitive-impairment-by-promoting-autophagic-clearance-and-inhibiting-production-of-%C3%AE-amyloid-in-app-tau-ps1-mouse-model-of-alzheimer-s-disease
#9
Min Huang, Xin Jiang, Yubin Liang, Qiong Liu, Siyan Chen, Yi Guo
This study investigates the neuroprotective properties of berberine (a natural isoquinoline alkaloid isolated from the Rhizoma coptidis) and finds that berberine could promote β-amyloid (Aβ) clearance and inhibit Aβ production in the triple-transgenic mouse model of Alzheimer's disease (3×Tg-AD). During the study, berberine was first administrated to treat 3×Tg-AD mice and primary neurons. Morris water maze assay, western blotting, enzyme-linked immunosorbent assay (ELISA), immunofluorescence staining and histological analysis, transmission electron microscopic analysis were then used to evaluate the effects of the berberine administration...
February 20, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28220899/group-a-streptococcal-m1-protein-provides-resistance-against-the-antimicrobial-activity-of-histones
#10
Simon Döhrmann, Christopher N LaRock, Ericka L Anderson, Jason N Cole, Brinda Ryali, Chelsea Stewart, Poochit Nonejuie, Joe Pogliano, Ross Corriden, Partho Ghosh, Victor Nizet
Histones are essential elements of chromatin structure and gene regulation in eukaryotes. An unexpected attribute of these nuclear proteins is their antimicrobial activity. A framework for histone release and function in host defense in vivo was revealed with the discovery of neutrophil extracellular traps, a specialized cell death process in which DNA-based structures containing histones are extruded to ensnare and kill bacteria. Investigating the susceptibility of various Gram-positive pathogens to histones, we found high-level resistance by one leading human pathogen, group A Streptococcus (GAS)...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220360/oral-histoplasmosis
#11
Gillian A Folk, Brenda L Nelson
A 44-year-old female presented to her general dentist with the chief complaint of a painful mouth sore of 2 weeks duration. Clinical examination revealed an irregularly shaped ulcer of the buccal and lingual attached gingiva of the anterior mandible. A biopsy was performed and microscopic evaluation revealed histoplasmosis. Histoplasmosis, caused by Histoplasma capsulate, is the most common fungal infection in the United States. Oral lesions of histoplasmosis are generally associated with the disseminated form of histoplasmosis and may present as a fungating or ulcerative lesion of the oral mucosa...
February 20, 2017: Head and Neck Pathology
https://www.readbyqxmd.com/read/28219304/solitary-thoracic-intramedullary-spinal-neurofibroma-microsurgically-extirpated-via-recapping-t-saw-laminoplasty
#12
Mamer Soriano Rosario, Hideki Murakami, Satoshi Kato, Moriyuki Fujii, Noritaka Yonezawa, Hiroyuki Tsuchiya
We report the case of a 40-year-old female presenting with back pain that was complicated by a solitary intramedullary spinal cord mass at the T10-11 levels, confirmed by magnetic resonance imaging and computed tomography myelography. Microsurgical en bloc extirpation of the tumor approached through a recapping T-saw laminoplasty of T10 was done, and histopathology findings revealed a diagnosis of neurofibroma. Solitary spinal neurofibroma is one of the rarest tumors involving the spinal cord and is very adherent for the lack of a well-defined capsule, requiring careful dissection under microscope magnification for successful en bloc resection...
January 2017: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/28217795/understanding-the-microscopic-binding-mechanism-of-hydroxylated-and-sulfated-polybrominated-diphenyl-ethers-with-transthyretin-by-molecular-docking-molecular-dynamics-simulations-and-binding-free-energy-calculations
#13
Huiming Cao, Yuzhen Sun, Ling Wang, Chunyan Zhao, Jianjie Fu, Aiqian Zhang
Polybrominated diphenyl ethers (PBDEs), one typical type of persistent environmental contaminant, have toxicological effects such as disrupting thyroid homeostasis in the human body. The high binding affinities of hydroxylated metabolites of PBDEs (OH-PBDEs) with transthyretin (TTR) were considered to be one major reason for their extraordinary capacity of passing through the blood-brain barrier via competitive thyroid hormone (T4) transport protein binding. Recent findings showed that sulfated PBDEs can be formed in human liver cytosol as phase-II metabolites...
February 20, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28217684/extrauterine-epithelioid-trophoblastic-tumor-in-hysterectomized-woman
#14
Ji-Hye Kim, Sun Kyung Lee, Soo Hyun Hwang, Jung-Sun Kim, Gun Yoon, Yoo-Young Lee, Tae-Joong Kim, Chel Hun Choi, Byoung-Gie Kim, Duk-Soo Bae, Jeong-Won Lee
Epithelioid trophoblastic tumor (ETT) is a very rare variant of gestational trophoblastic disease (GTD) which arises in reproductive age women with prior gestational history. Although abnormal vaginal bleeding is the most common symptom of ETT, there are no reported pathognomonic symptoms of ETT because of its rarity. ETT is similar to placental site trophoblastic tumor in terms of its slow growing characteristic and microscopic findings. Therefore, it could be misdiagnosed as placental site trophoblastic tumor or other types of GTD...
January 2017: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/28217470/a-case-report-of-reactive-solitary-eccrine-syringofibroadenoma
#15
Anup K Tiwary, J Firdous, Dharmendra K Mishra, Shyam S Chaudhary
Eccrine syringofibroadenoma is a very rare benign tumour of acrosyringium of eccrine sweat duct. Based on the evidences of known etiological factors, two forms have been proposed; reactive and nonreactive. Reactive forms are rarer, and on even rarer occasions, trauma complicated by secondary nonspecific infections may lead to the development of reactive eccrine syringofibroadenoma, as in our case. Here, we are documenting a case of reactive solitary eccrine syringofibroadenoma in a 65-year-old male presenting with coalescing, firm, pinkish, verrucous nodules and painful deep ulceration on the right sole preceded by trauma and secondary infection...
January 2017: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/28217393/rare-association-of-secondary-superficial-siderosis-caused-by-a-fourth-ventricle-hemorrhagic-ependymoma-mimicking-a-cavernoma-case-report-and-literature-review
#16
Eduardo E Espinosa Rodríguez, Rodrigo Carrasco Moro, Juan S Martínez San Millán, Héctor G Pian Arias
BACKGROUND: The association of a hemorrhagic tumor with secondary superficial siderosis (SS) is a relatively rare although well described phenomenon. CASE DESCRIPTION: We present the case report of a 35-year-old male with a history of drowsiness, hypoacusia, drop attacks, and multidirectional nystagmus during the last 2 months, who presented with acute obstructive hydrocephalus caused by a fourth ventricle mass displaying radiological signs of repeated intra and extratumoral hemorrhage with SS...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28215538/over-90-of-cases-of-microscopic-colitis-can-be-diagnosed-by-performing-a-short-colonoscopy
#17
Gilles Macaigne, Pierre Lahmek, Christophe Locher, Jean François Boivin, Bruno Lesgourgues, Mathieu Yver, Laurent Costes, I Abd Alsamad, Joel Cucherousset, Claire Charpignon, Hélène Guyot, Bénédicte Lambaré, Jean-Michel Ghilain, Valérie Calès, Stéphanie de Montigny-Lenhardt, Guy Bellaïche, Alexandre Pariente, Stéphane Nahon
AIMS: To determinate the topographical distribution of key diagnostic histological features of lymphocytic colitis (LC) and collagenous colitis (CC) and to establish what correlations may exist between the histological findings and the causes and severity of MC. PATIENTS AND METHODS: Patients with MC were included in a prospective multicentre French study from September 2010 to October 2012. MC was diagnosed by performing total colonoscopy with multiple biopsies of the rectum and colon collected in separate jars and analyzed separately for each site (descending and sigmoid colon, transverse colon, ascending colon)...
February 16, 2017: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/28214756/multifarious-applications-of-atomic-force-microscopy-in-forensic-science-investigations
#18
REVIEW
Gaurav Pandey, Maithri Tharmavaram, Deepak Rawtani, Sumit Kumar, Y Agrawal
Forensic science is a wide field comprising of several subspecialties and uses methods derived from natural sciences for finding criminals and other evidence valid in a legal court. A relatively new area; Nano-forensics brings a new era of investigation in forensic science in which instantaneous results can be produced that determine various agents such as explosive gasses, biological agents and residues in different crime scenes and terrorist activity investigations. This can be achieved by applying Nanotechnology and its associated characterization techniques in forensic sciences...
February 11, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28213826/diagnostic-yield-of-ct-urography-in-the-evaluation-of-hematuria-in-young-patients-in-a-military-population
#19
Laura R Mace, Terrel L Galloway, Andrew Ma, Richard S Montgomery, Ryan C Rockhill, William W Reynolds, Robert M Marks
PURPOSE: To assess the diagnostic yield of a computed tomography urography (CTU) study in patients less than 50 years of age, who have a history of military service, and who are at increased risk of urological cancers secondary to harmful practices and work-related exposures. METHODS: 137 Consecutive patients who underwent CTU between 2012 and 2013 for new onset of hematuria were included. Initial review of the clinical interpretations of the CTU studies grouped the studies into negative and positive exams for any urological findings...
February 17, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28211931/the-dynamic-conductance-response-and-mechanics-modulated-memristive-behavior-of-the-azurin-monolayer-under-cyclic-loads
#20
Xiaoyue Zhang, Jian Shao, Yun Chen, Weijin Chen, Jing Yu, Biao Wang, Yue Zheng
Azurin (Az) has been considered as the research hotspot in molecular electronics, as well as a promising material for building functional devices on the molecular scale because of its special electrical properties and force-dependent conductance effects. Here we carry out an in-depth investigation combined with molecular scale experiments, molecular dynamics simulations, first-principles calculations and theoretical models to reveal the dynamic conductance response of the Az monolayer under cyclic mechanical loading...
February 17, 2017: Physical Chemistry Chemical Physics: PCCP
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