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https://www.readbyqxmd.com/read/28937997/molecular-detection-of-borrelia-burgdorferi-sensu-lato-an-analytical-comparison-of-real-time-pcr-protocols-from-five-different-scandinavian-laboratories
#1
Malin Lager, Maximilian Faller, Peter Wilhelmsson, Vivian Kjelland, Åshild Andreassen, Rimtas Dargis, Hanne Quarsten, Ram Dessau, Volker Fingerle, Gabriele Margos, Sølvi Noraas, Katharina Ornstein, Ann-Cathrine Petersson, Andreas Matussek, Per-Eric Lindgren, Anna J Henningsson
INTRODUCTION: Lyme borreliosis (LB) is the most common tick transmitted disease in Europe. The diagnosis of LB today is based on the patient´s medical history, clinical presentation and laboratory findings. The laboratory diagnostics are mainly based on antibody detection, but in certain conditions molecular detection by polymerase chain reaction (PCR) may serve as a complement. AIM: The purpose of this study was to evaluate the analytical sensitivity, analytical specificity and concordance of eight different real-time PCR methods at five laboratories in Sweden, Norway and Denmark...
2017: PloS One
https://www.readbyqxmd.com/read/28927817/development-and-evaluation-of-a-two-step-multiplex-taqman-real-time-pcr-assay-for-detection-quantification-of-different-genospecies-of-borrelia-burgdorferi-sensu-lato
#2
Mónica Nunes, Ricardo Parreira, Teresa Carreira, João Inácio, Maria Luísa Vieira
Nowadays, at least four clinically important B. burgdorferi sensu lato (s.l.) genospecies (B. afzelii, B. garinii, B. burgdorferi sensu stricto (s.s.) and B. lusitaniae) circulate in Portugal. Each genospecies has a different tropism that resuls in a diverse array of clinical manifestations. The standard diagnostic procedure used is normally simple, nevertheless, during the "window-period" phase, in which specific antibodies cannot yet be detected, diagnosis becomes difficult, and calls for reliable, sensitive and specific laboratory methods, such as molecular tests...
September 11, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28918538/genetic-manipulation-of-borrelia-spp
#3
Dan Drecktrah, D Scott Samuels
The spirochetes Borrelia (Borreliella) burgdorferi and Borrelia hermsii, the etiologic agents of Lyme disease and relapsing fever, respectively, cycle in nature between an arthropod vector and a vertebrate host. They have extraordinarily unusual genomes that are highly segmented and predominantly linear. The genetic analyses of Lyme disease spirochetes have become increasingly more sophisticated, while the age of genetic investigation in the relapsing fever spirochetes is just dawning. Molecular tools available for B...
September 17, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28918069/studies-that-report-unexpected-positive-blood-cultures-for-lyme-borrelia-are-they-valid
#4
REVIEW
Gary P Wormser, Eugene D Shapiro, Franc Strle
Positive blood cultures for Lyme borrelia have been well documented in untreated patients with early Lyme disease. In this report we review the validity of three studies that reported the recovery of Borrelia burgdorferi sensu lato from the blood of a high proportion of patients for whom no evidence was presented, and no claim was made, that the patients had untreated early Lyme disease. In two of the studies the patients had been treated extensively with antibiotics for Lyme disease before the cultures were obtained...
July 29, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28917304/prevalence-of-ticks-and-tick-borne-pathogens-babesia-and-borrelia-species-in-ticks-infesting-cats-of-great-britain
#5
Saran Davies, Swaid Abdullah, Chris Helps, Séverine Tasker, Hannah Newbury, Richard Wall
In a study of tick and tick-borne pathogen prevalence, between May and October 2016, 278 veterinary practices in Great Britain examined 1855 cats. Six-hundred and one cats were found to have attached ticks. The most frequently recorded tick species was Ixodes ricinus (57.1%), followed by Ixodes hexagonus (41.4%) and Ixodes trianguliceps (1.5%). Male cats, 4-6 years of age living in rural areas were most likely to be carrying a tick; hair length and tick treatment history had no significant association with attachment...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28911844/serologic-responses-to-peptides-of-anaplasma-phagocytophilum-and-borrelia-burgdorferi-in-dogs-infested-with-wild-caught-ixodes-scapularis
#6
R Chandrashekar, M J Beall, B Thatcher, J M Saucier, P Tyrrell, M R Lappin
Anaplasma phagocytophilum and Borrelia burgdorferi are both transmitted by Ixodes spp. and are associated with clinical illness in some infected dogs. This study evaluated canine antibody responses to the A. phagocytophilum p44 peptides APH-1 and APH-4 as well as the B. burgdorferi C6 peptide before and after doxycycline treatment. A total of eight dogs were infested with wild-caught I. scapularis for 1 week. Blood was collected prior to tick attachment and from Days 3-77 to 218-302 with doxycycline treatment beginning on Day 218...
August 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28911326/borrelia-infection-and-risk-of-celiac-disease
#7
Armin Alaedini, Benjamin Lebwohl, Gary P Wormser, Peter H Green, Jonas F Ludvigsson
BACKGROUND: Environmental factors, including infectious agents, are speculated to play a role in the rising prevalence and the geographic distribution of celiac disease, an autoimmune disorder. In the USA and Sweden where the regional variation in the frequency of celiac disease has been studied, a similarity with the geographic distribution of Lyme disease, an emerging multisystemic infection caused by Borrelia burgdorferi spirochetes, has been found, thus raising the possibility of a link...
September 15, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28905981/-heterogeneity-of-canine-immune-responses-to-borrelia-burgdorferi-in-a-line-immunoassay-comprising-recombinant-vlse-and-c6-peptide
#8
Doris Breu, Elisabeth Müller
OBJECTIVE: The study aimed to investigate the distribution of specific immune responses (IgG) to Borrelia burgdorferi using a line immunoassay with recombinant VlsE (variable major protein-like sequence, expressed) protein and synthetic C peptide among other antigens. We compared the immune responses to VlsE protein and C6 peptide, because both antigens have been considered specific for a Borrelia infection. MATERIALS AND METHODS: A total of 1355 blood samples from dogs suspected of Borrelia infection were analysed...
September 13, 2017: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/28898274/periplasmic-flagella-in-borrelia-burgdoferi-function-to-maintain-cellular-integrity-upon-external-stress
#9
Bharath Kumar, Kelly Miller, Nyles W Charon, Justin Legleiter
Tapping mode atomic force microscopy (AFM) in solution was used to analyze the role of the internally located periplasmic flagella (PFs) of the Lyme disease spirochete Borrelia burgdorferi in withstanding externally applied cellular stresses. By systematically imaging immobilized spirochetes with increasing tapping forces, we found that mutants that lack PFs are more readily compressed and damaged by the imaging process compared to wild-type cells. This finding suggest that the PFs, aside from being essential for motility and involved in cell shape, play a cytoskeletal role in dissipating energy and maintaining cellular integrity in the presence of external stress...
2017: PloS One
https://www.readbyqxmd.com/read/28887186/to-test-or-not-to-test-laboratory-support-for-the-diagnosis-of-lyme-borreliosis
#10
REVIEW
Ram B Dessau, Alje P van Dam, Volker Fingerle, Jeremy Gray, Joppe Hovius, Klaus-Peter Hunfeld, Benoit Jaulhac, Olaf Kahl, Wolfgang Kristoferitsch, Per-Eric Lindgren, Mateusz Markowicz, Sally Mavin, Katharina Ornstein, Tobias Rupprecht, Gerold Stanek, Franc Strle
BACKGROUND: Lyme borreliosis (LB) is a tick-borne infection caused by Borrelia burgdorferi sensu lato. The most frequent clinical manifestations are erythema migrans and Lyme neuroborreliosis. Currently, a large volume of diagnostic testing for Lyme borreliosis is reported, whereas the incidence of clinically relevant disease manifestations is low. This indicates overuse of diagnostic testing for LB with implications for patient care and cost effective health management. AIM: The recommendations provided in this review are intended to support both the clinical diagnosis and initiatives for a more rational use of laboratory testing in patients with clinically suspected Lyme borreliosis...
September 5, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28884668/borrelia-lanei-sp-nov-extends-the-diversity-of-borrelia-species-in-california
#11
Gabriele Margos, Natalia Fedorova, Joyce E Kleinjan, Christine Hartberger, Tom G Schwan, Andreas Sing, Volker Fingerle
The diversity of Borrelia species discovered in California appears to be particularly high. A divergent group of Borrelia strains collected from Ixodes ticks in California was described by Postic and co-workers and designated 'genomospecies 2' (Postic D, Garnier M, Baranton G. Int J Med Microbiol 2007;297:263-271; Postic D, Ras NM, Lane RS, Hendson M, Baranton G. J Clin Microbiol 1998;36:3497-3504). We performed multilocus sequence analysis (MLSA) using eight housekeeping loci (clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA) on 12 strains of this Borreliagenospecies to confirm that these strains form a distinct group within the Borreliaburgdorferi s...
September 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28882852/human-seroprevalence-of-borrelia-miyamotoi-in-manitoba-canada-in-2011-2014-a-cross-sectional-study
#12
Kamran Kadkhoda, Cecilia Dumouchel, Janna Brancato, Ainsley Gretchen, Peter J Krause
BACKGROUND: Hard tick-borne relapsing fever caused by Borrelia miyamotoi has been reported in Russia, the Netherlands, Germany, Japan and the northeastern and upper midwestern United States. We sought to investigate the presence of B. miyamotoi infection in humans in Manitoba, Canada. METHODS: Two hundred fifty sera collected from residents of Manitoba with suspected Lyme disease between 2011 and 2014 were tested for Borrelia burgdorferi antibody using a C6 peptide enzyme-linked immunosorbent assay (ELISA) followed by Western blot...
September 6, 2017: CMAJ Open
https://www.readbyqxmd.com/read/28878005/citrate-anticoagulant-improves-the-sensitivity-of-borreliella-borrelia-burgdorferi-plasma-culture
#13
Adriana R Marques, Xiuli Yang, Alexis A Smith, Xuran Zhuang, Siu-Ping Turk, Carla D Williams, Melissa A Law, Alan G Barbour, Utpal Pal
The ability of cultivating Borreliella (Borrelia) burgdorferi is a cornerstone of Lyme disease research.….
September 6, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28875270/molecular-evidence-of-tick-borne-pathogens-in-hyalomma-anatolicum-ticks-infesting-cattle-in-xinjiang-uygur-autonomous-region-northwestern-china
#14
Peifa Yu, Zhijie Liu, Qingli Niu, Jifei Yang, Mirza Omar Abdallah, Ze Chen, Guangyuan Liu, Jianxun Luo, Hong Yin
Although tick-borne pathogens have been widely reported in ticks in China, there is little information available on the prevalence of information in Hyalomma ticks from cattle. This study aims to determine the occurrence of pathogens in Hyalomma anatolicum collected from cattle in Xinjiang Uygur Autonomous Region, China, by PCR, sequencing and phylogenetic analysis. Borrelia burgdorferi s.s., Rickettsia massiliae and Anaplasma bovis were identified, whereas DNA of Ehrlichia species and an Anaplasma platys-like pathogen were also detected...
September 5, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28874457/correction-for-chenail-et-al-borrelia-burgdorferi-cp32-bpab-modulates-expression-of-the-prophage-nucp-nuclease-and-ssbp-single-stranded-dna-binding-protein
#15
Alicia M Chenail, Brandon L Jutras, Claire A Adams, Logan H Burns, Amy Bowman, Ashutosh Verma, Brian Stevenson
No abstract text is available yet for this article.
October 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28874456/correction-for-jutras-et-al-changes-in-bacterial-growth-rate-govern-expression-of-the-borrelia-burgdorferi-ospc-and-erp-infection-associated-surface-proteins
#16
Brandon L Jutras, Alicia M Chenail, Brian Stevenson
No abstract text is available yet for this article.
October 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28874016/transmission-of-the-lyme-disease-spirochete-borrelia-mayonii-in-relation-to-duration-of-attachment-by-nymphal-ixodes-scapularis-acari-ixodidae
#17
Marc C Dolan, Nicole E Breuner, Andrias Hojgaard, Karen A Boegler, J Charles Hoxmeier, Adam J Replogle, Lars Eisen
The recently recognized Lyme disease spirochete, Borrelia mayonii, has been detected in host-seeking Ixodes scapularis Say ticks and is associated with human disease in the Upper Midwest. Although experimentally shown to be vector competent, studies have been lacking to determine the duration of time from attachment of a single B. mayonii-infected I. scapularis nymph to transmission of spirochetes to a host. If B. mayonii spirochetes were found to be transmitted within the first 24 h after tick attachment, in contrast to Borrelia burgdorferi spirochetes (>24 h), then current recommendations for tick checks and prompt tick removal as a way to prevent transmission of Lyme disease spirochetes would need to be amended...
September 1, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28873507/borrelia-burgdorferi-outer-surface-protein-c-ospc-binds-complement-component-c4b-and-confers-bloodstream-survival
#18
Jennifer A Caine, Yi-Pin Lin, Julie R Kessler, Hiromi Sato, John M Leong, Jenifer Coburn
Borrelia burgdorferi (Bb) is the causative agent of Lyme disease in the US, a disease that can result in carditis, and chronic and debilitating arthritis and/or neurologic symptoms if left untreated. Bb survives in the midgut of the Ixodes scapularis tick, or within tissues of immunocompetent hosts. In the early stages of infection, the bacteria are present in the bloodstream where they must resist clearance by the innate immune system of the host. We have found a novel role for OspC from B. burgdorferi and B...
September 5, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28867633/borrelia-burgdorferi-basic-membrane-protein-a-could-induce-chemokine-production-in-murine-microglia-cell-line-bv2
#19
Hua Zhao, Aihua Liu, Yuhui Cui, Zhang Liang, Bingxue Li, Fukai Bao
Lyme neuroborreliosis is a nervous system infectious disease caused by Borrelia burgdorferi (B. burgdorferi). It has been demonstrated that cytokines induced by B. burgdorferi are related to Lyme neuroborreliosis. Microglia is known as a key player in the immune responses that occur within the central nervous system. In response to inflammation, it will be activated and generate cytokines and chemokines. Experiments in vitro cells have showed that B. Burgdorferi membrane protein A (BmpA), a major immunogen of B...
September 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28867507/immunization-of-mice-with-borrelia-burgdorferi-lp54-gene-encoded-recombinant-proteins-does-not-provide-protection-against-tick-transmitted-infectious-challenge
#20
Kevin S Brandt, Robert D Gilmore
The Borrelia burgdorferi outer surface membrane proteins BBA65, BBA66, BBA69, BBA70, and BBA73 were tested for their ability to confer protection against B. burgdorferi infection challenge. Mice were immunized with recombinant forms of the proteins singly or in combinations. Following initial protein inoculation and booster injections, seroconversion was confirmed prior to B. burgdorferi challenge by tick bite. Despite mice having high antibody titers for each antigen, no significant protections against the challenge infections were observed...
September 25, 2017: Vaccine
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