Yutaka Tomita, Elisabeth Pinard, Alexy Tran-Dinh, Istvan Schiszler, Nathalie Kubis, Minoru Tomita, Norihiro Suzuki, Jacques Seylaz
A method for long-term, repeated, semi-quantitative measurements of cerebral microflow at the same region of interest (ROI) with high spatial resolution was developed and applied to mice subjected to focal arterial occlusion. A closed cranial window was chronically implanted over the left parieto-occipital cortex. The anesthetized mouse was placed several times, e.g., weekly, under a dynamic confocal microscope, and Rhodamine B-isothiocyanate-dextran was each time intravenously injected as a bolus, while microflow images were video recorded...
February 4, 2011: Brain Research