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Salivary proteomics

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https://www.readbyqxmd.com/read/28055279/salivary-protein-changes-in-response-to-acute-stress-in-medical-residents-performing-advanced-clinical-simulations-a-pilot-proteomics-study
#1
Rachel K Marvin, Muncharie B Saepoo, Simiao Ye, Donald B White, Rong Liu, Kenneth Hensley, Paul Rega, Viviane Kazan, David R Giovannucci, Dragan Isailovic
CONTEXT: Quantitative changes of salivary proteins due to acute stress were detected. OBJECTIVE: To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations. MATERIALS AND METHODS: Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by SDS-PAGE, identified by MS, and relatively quantified by densitometry. RESULTS: Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (<10 kDa) SDS-PAGE bands exhibited changes after stress...
January 5, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/28049606/an-insight-into-the-sialome-of-hyalomma-excavatum
#2
José M C Ribeiro, Mirko Slovák, Ivo M B Francischetti
Tick saliva contains hundreds or thousands of proteins that help blood feeding by impairing their hosts' hemostasis, inflammation and immunity. Salivary gland transcriptomes allow the disclosure of this pharmacologically active potion that consists of several multi-gene families, many of which are tick-specific. We here report the "de novo" assembly of ∼138 million reads deriving from a cDNA library from salivary glands of adult male and female Hyalomma excavatum leading to the public deposition of 5337 coding sequences to GenBank...
December 21, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28018380/functional-evaluation-of-proteins-in-watery-and-gel-saliva-of-aphids
#3
REVIEW
Aart J E van Bel, Torsten Will
Gel and watery saliva are regarded as key players in aphid-pIant interactions. The salivary composition seems to be influenced by the variable environment encountered by the stylet tip. Milieu sensing has been postulated to provide information needed for proper stylet navigation and for the required switches between gel and watery saliva secretion during stylet progress. Both the chemical and physical factors involved in sensing of the stylet's environment are discussed. To investigate the salivary proteome, proteins were collected from dissected gland extracts or artificial diets in a range of studies...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27978919/-current-advance-in-saliva-derived-exosome-study
#4
Y Zhao, L Y Yin, R R Bi, P Ma, S B Li
There is growing interest in the whole saliva as a diagnostic fluid because of the relatively simple and non-invasive collection. Research on salivary proteomic and genomic has greatly facilitated the diagnosis and therapy of oral diseases, also revealed the molecular mechanism of the diseases. Saliva-derived exosome as a new source, with various molecular constituents of their cells of origin, including proteins, mRNA and miRNA might serve as potential biomarker. This article reviews the biological properties of saliva exosome, its separation and other aspects associated with disease...
December 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27976689/the-proteomic-profile-of-whole-and-glandular-saliva-in-healthy-pain-free-subjects
#5
Hajer Jasim, Patrik Olausson, Britt Hedenberg-Magnusson, Malin Ernberg, Bijar Ghafouri
Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum to be able to reproducibly assess variability in states of health and disease. In the current study, the proteome profile in unstimulated and stimulated whole, parotid and sublingual saliva was investigated using two-dimensional gel electrophoresis...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27966577/salivary-and-pellicle-proteome-a-datamining-analysis
#6
Hardy Schweigel, Michael Wicht, Falk Schwendicke
We aimed to comprehensively compare two compartmented oral proteomes, the salivary and the dental pellicle proteome. Systematic review and datamining was used to obtain the physico-chemical, structural, functional and interactional properties of 1,515 salivary and 60 identified pellicle proteins. Salivary and pellicle proteins did not differ significantly in their aliphatic index, hydrophaty, instability index, or isoelectric point. Pellicle proteins were significantly more charged at low and high pH and were significantly smaller (10-20 kDa) than salivary proteins...
December 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27943336/verification-of-protein-biomarkers-for-the-identification-of-biological-stains-by-quadrupole-time-of-flight-mass-spectrometry
#7
Kevin M Legg, Roger Powell, Nichole Reisdorph, Rick Reisdorph, Phillip B Danielson
Advances in proteomics technology over the past decade offer forensic serologists a greatly improved opportunity to accurately characterize the tissue source from which a DNA has been developed. Such information can provide critical context to evidence and can help to prioritize downstream DNA analysis. Previous proteome studies compiled panels of "candidate biomarkers" specific to each of six body fluids (i.e. peripheral blood, menstrual fluid, vaginal fluid, seminal fluid, urine and saliva). Here, a multiplex Quadrupole Time-of-flight assay has been developed in order to verify the tissue/body fluid specificity the 23 protein biomarkers that comprise these panels and the consistency with which they can be detected across a samples population of fifty humans...
December 12, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27943101/salivary-biomarkers-in-cancer-detection
#8
REVIEW
Xiaoqian Wang, Karolina Elżbieta Kaczor-Urbanowicz, David T W Wong
Cancer is the second most common cause of death in the USA. Its symptoms are often not specific and absent, until the tumors have already metastasized. Therefore, there is an urgent demand for developing rapid, highly accurate and noninvasive tools for cancer screening, early detection, diagnostics, staging and prognostics. Saliva as a multi-constituent oral fluid comprises secretions from the major and minor salivary glands, extensively supplied by blood. Molecules such as DNAs, RNAs, proteins, metabolites, and microbiota, present in blood, could be also found in saliva...
January 2017: Medical Oncology
https://www.readbyqxmd.com/read/27903834/saliva-diagnostics-current-views-and-directions
#9
Karolina Elżbieta Kaczor-Urbanowicz, Carmen Martin Carreras-Presas, Katri Aro, Michael Tu, Franklin Garcia-Godoy, David Tw Wong
In this review, we provide an update on the current and future applications of saliva for diagnostic purposes. There are many advantages of using saliva as a biofluid. Its collection is fast, easy, inexpensive, and non-invasive. In addition, saliva, as a "mirror of the body," can reflect the physiological and pathological state of the body. Therefore, it serves as a diagnostic and monitoring tool in many fields of science such as medicine, dentistry, and pharmacotherapy. Introduced in 2008, the term "Salivaomics" aimed to highlight the rapid development of knowledge about various "omics" constituents of saliva, including: proteome, transcriptome, micro-RNA, metabolome, and microbiome...
November 29, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27900324/low-expression-of-angiotensinogen-and-dipeptidyl-peptidase-1-in-saliva-of-patients-with-proliferative-verrucous-leukoplakia
#10
Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Adriana Franco Paes Leme, Marcio Ajudarte Lopes
AIM: To elucidate the profile of the salivary proteome. METHODS: Unstimulated whole mouth saliva was collected from 30 volunteers [15 proliferative verrucous leukoplakia (PVL) patients and 15 controls] and proteins were submitted for mass spectrometry-based proteomics using the discovery approach, followed by analyses of variance and logistic regression tests. RESULTS: A total of two hundred and eighty-three proteins were confidently identified in saliva...
November 16, 2016: World Journal of Clinical Cases
https://www.readbyqxmd.com/read/27891302/epidemiology-of-sj%C3%A3-gren-s-syndrome-from-an-oral-perspective
#11
REVIEW
Anne Isine Bolstad, Kathrine Skarstein
Oral symptoms are among the most distressing manifestations for patients with Sjögren's syndrome (SS). The feeling of dry mouth is unpleasant, and hyposalivation may contribute to difficulty in speaking, chewing and swallowing and reduced quality of life. Reduced salivary flow increases the risk for dental caries and problems with prosthetic replacement. It seems that SS is not as frequently occurring as previously anticipated. Population-based prevalence studies on primary SS in Europe, conducted on large background populations and in accordance with the AECG criteria, reported of a prevalence of 1-9 cases per 10,000 people...
2016: Current Oral Health Reports
https://www.readbyqxmd.com/read/27887661/biochemical-and-proteomic-analyses-of-the-physiological-response-induced-by-individual-housing-in-gilts-provide-new-potential-stress-markers
#12
Anna Marco-Ramell, Laura Arroyo, Raquel Peña, Raquel Pato, Yolanda Saco, Lorenzo Fraile, Emøke Bendixen, Anna Bassols
BACKGROUND: The objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort. RESULTS: Blood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals...
November 25, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27802913/proteomic-analysis-of-human-saliva-an-approach-to-find-the-marker-protein-for-ovulation
#13
Ganesan Saibaba, Durairaj Rajesh, Subramanian Muthukumar, Ganesan Sathiyanarayanan, Parasuraman Padmanabhan, Mohammad Abdulkader Akbarsha, Balázs Gulyás, Govindaraju Archunan
Human saliva contains numerous molecules that play a variety of roles. Among them there are proteins which serve as biomarkers of various physiological and/or pathological conditions. Compared to other body fluids, saliva is the most convenient material for investigations, and especially for monitoring the disease conditions. Presently, there is an increasing need to develop a noninvasive method to identify the time of ovulation in humans to ensure successful fertilization, and for evolving strategies for family planning...
December 2016: Reproductive Biology
https://www.readbyqxmd.com/read/27789276/tear-fluid-protein-changes-in-dry-eye-syndrome-associated-with-rheumatoid-arthritis-a-proteomic-approach
#14
Saijyothi Venkata Aluru, Agarwal Shweta, Srinivasan Bhaskar, Krishnan Geetha, Rajappa M Sivakumar, Tatu Utpal, Prema Padmanabhan, Narayanasamy Angayarkanni
PURPOSE: Sjögren syndrome (SS) secondary to rheumatoid arthritis (RA) affects lacrimal and salivary glands, and therefore dry eye syndrome (DES) is more prevalent in patients with RA. This study used a proteomic approach to identify potential biomarkers in tear of DES secondary to RA (DES-RA). METHODS: Tear specimens were collected with Schirmer strips from patients with DES with RA, patients with other types of dry eye (namely, primary Sjögrens and non-Sjögrens [NSS]), and age-matched controls...
January 2017: Ocular Surface
https://www.readbyqxmd.com/read/27716395/thymosin-%C3%AE-4-and-%C3%AE-10-in-sj%C3%A3-gren-s-syndrome-saliva-proteomics-and-minor-salivary-glands-expression
#15
Silvia Bosello, Giusy Peluso, Federica Iavarone, Barbara Tolusso, Irene Messana, Gavino Faa, Massimo Castagnola, Gianfranco Ferraccioli
BACKGROUND: In the present study, we investigated whether thymosin β (Tβ) in saliva and in minor salivary glands is differentially expressed in patients with primary Sjögren's syndrome (pSS) and patients with autoimmune diseases (systemic sclerosis [SSc], systemic lupus erythematosus [SLE], and rheumatoid arthritis [RA], with and without sicca syndrome [ss]). METHODS: Saliva specimens of nine patients with pSS, seven with ss/SSc, seven with ss/SLE, seven with ss/RA, seven with SSc, seven with SLE, and seven with RA, as well as ten healthy subjects, were analyzed using a high-performance liquid chromatograph coupled with a mass spectrometer equipped with an electrospray ionization source to investigate the presence and levels of Tβ4, Tβ4 sulfoxide, and Tβ10...
October 6, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27714937/plasmosep-predicting-surface-exposed-proteins-on-the-malaria-parasite-using-semisupervised-self-training-and-expert-annotated-data
#16
Yasser El-Manzalawy, Elyse E Munoz, Scott E Lindner, Vasant Honavar
Accurate and comprehensive identification of surface-exposed proteins (SEPs) in parasites is a key step in developing novel subunit vaccines. However, the reliability of MS-based high-throughput methods for proteome-wide mapping of SEPs continues to be limited due to high rates of false positives (i.e., proteins mistakenly identified as surface exposed) as well as false negatives (i.e., SEPs not detected due to low expression or other technical limitations). We propose a framework called PlasmoSEP for the reliable identification of SEPs using a novel semisupervised learning algorithm that combines SEPs identified by high-throughput experiments and expert annotation of high-throughput data to augment labeled data for training a predictive model...
December 2016: Proteomics
https://www.readbyqxmd.com/read/27703040/the-salivary-protein-repertoire-of-the-polyphagous-spider-mite-tetranychus-urticae-a-quest-for-effectors
#17
Wim Jonckheere, Wannes Dermauw, Vladimir Zhurov, Nicky Wybouw, Jan Van den Bulcke, Carlos A Villarroel, Robert Greenhalgh, Mike Grbić, Rob C Schuurink, Luc Tirry, Geert Baggerman, Richard M Clark, Merijn R Kant, Bartel Vanholme, Gerben Menschaert, Thomas Van Leeuwen
The two-spotted spider mite Tetranychus urticae is an extremely polyphagous crop pest. Alongside an unparalleled detoxification potential for plant secondary metabolites, it has recently been shown that spider mites can attenuate or even suppress plant defenses. Salivary constituents, notably effectors, have been proposed to play an important role in manipulating plant defenses and might determine the outcome of plant-mite interactions. Here, the proteomic composition of saliva from T. urticae lines adapted to various host plants-bean, maize, soy, and tomato-was analyzed using a custom-developed feeding assay coupled with nano-LC tandem mass spectrometry...
December 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27676445/bottom-up-proteomics-suggests-an-association-between-differential-expression-of-mitochondrial-proteins-and-chronic-fatigue-syndrome
#18
F Ciregia, L Kollipara, L Giusti, R P Zahedi, C Giacomelli, M R Mazzoni, G Giannaccini, P Scarpellini, A Urbani, A Sickmann, A Lucacchini, L Bazzichi
Chronic fatigue syndrome (CFS) is a debilitating and complex disorder characterized by unexplained fatigue not improved by rest. An area of investigation is the likely connection of CFS with defective mitochondrial function. In a previous work, we investigated the proteomic salivary profile in a couple of monozygotic twins discordant for CFS. Following this work, we analyzed mitochondrial proteins in the same couple of twins. Nano-liquid chromatography electrospray ionization mass spectrometry (nano-LC-MS) was used to study the mitochondria extracted from platelets of the twins...
September 27, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27672500/metaproteomics-of-saliva-identifies-human-protein-markers-specific-for-individuals-with-periodontitis-and-dental-caries-compared-to-orally-healthy-controls
#19
Daniel Belstrøm, Rosa R Jersie-Christensen, David Lyon, Christian Damgaard, Lars J Jensen, Palle Holmstrup, Jesper V Olsen
BACKGROUND: The composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. To identify characteristics of diseased and healthy saliva we thus wanted to compare saliva metaproteomes from patients with periodontitis and dental caries to healthy individuals. METHODS: Stimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals...
2016: PeerJ
https://www.readbyqxmd.com/read/27669021/salivary-gland-proteome-during-adult-development-and-after-blood-feeding-of-female-anopheles-dissidens-mosquitoes-diptera-culicidae
#20
Benjarat Phattanawiboon, Narissara Jariyapan, Chonlada Mano, Sittiruk Roytrakul, Atchara Paemanee, Sriwatapron Sor-Suwan, Patchara Sriwichai, Atiporn Saeung, Paul A Bates
Understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. Anopheles dissidens (formerly Anopheles barbirostris species A1) is a potential vector of Plasmodium vivax in Thailand. In this study, analyses of the proteomic profiles of female An. dissidens salivary glands during adult development and after blood feeding were carried out using two-dimensional gel electrophoresis coupled with nano-liquid chromatography-mass spectrometry...
2016: PloS One
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