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Salivary proteomics

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https://www.readbyqxmd.com/read/28334389/profiling-diuraphis-noxia-hemiptera-aphididae-transcript-expression-of-the-biotypes-sa1-and-sam-feeding-on-various-triticum-aestivum-varieties
#1
N F V Burger, E Venter, A-M Botha
The intimate relationship between an aphid and its host is mediated by the composition of the secreted saliva. In the present study, aphid heads were sampled and transcript profiling conducted after aphids were fed on their preference host and transferred to a variety of preference and nonpreference hosts. It was found that the virulent Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) biotype SAM was able to selectively up-regulate more transcripts when confronted with feeding on a variety of hosts, than was the case with the less virulent D...
March 3, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28282148/alterations-in-the-salivary-proteome-and-n-glycome-of-sj%C3%A3-gren-s-syndrome-patients
#2
Steven C Hall, Maria E Hassis, Katherine E Williams, Matthew E Albertolle, Akraporn Prakobphol, Andrew B Dykstra, Megan Laurance, Katherine Ona, Richard K Niles, Namrata Prasad, Matthew Gormley, Caroline H Shiboski, Lindsey A Criswell, H Ewa Witkowska, Susan J Fisher
We used isobaric mass tagging (iTRAQ) and lectin affinity capture mass spectrometry (MS)-based workflows for global analyses of parotid saliva (PS) and whole saliva (WS) samples obtained from patients diagnosed with primary Sjögren's Syndrome (pSS) who were enrolled in the Sjögren's International Collaborative Clinical Alliance (SICCA) as compared to two control groups. The iTRAQ analyses revealed up- and down-regulation of numerous proteins that could be involved in the disease process (e.g., histones) or attempts to mitigate the ensuing damage [e...
March 10, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28271980/mosquito-borne-diseases-and-omics-salivary-gland-proteome-of-the-female-aedes-aegypti-mosquito
#3
Rakhi Dhawan, Manish Kumar, Ajeet Kumar Mohanty, Gourav Dey, Jayshree Advani, T S Keshava Prasad, Ashwani Kumar
The female Aedes aegypti mosquito is an important vector for several tropical and subtropical diseases such as dengue, chikungunya, and Zika and yellow fever. The disease viruses infect the mosquito and subsequently spread to the salivary glands after which the viruses can be transmitted to humans with probing or feeding by the mosquito. Omics systems sciences offer the opportunity to characterize vectors and can inform disease surveillance, vector control and development of innovative diagnostics, personalized medicines, vaccines, and insecticide targets...
January 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28249646/chromosomal-abnormalities-and-molecular-landscape-of-metastasizing-mucinous-salivary-adenocarcinoma
#4
Alex Panaccione, Yi Zhang, Yanfang Mi, Yoshitsugu Mitani, Guo Yan, Manju L Prasad, W Hayes McDonald, Adel K El-Naggar, Wendell G Yarbrough, Sergey V Ivanov
BACKGROUND: Mucinous adenocarcinoma of the salivary gland (MAC) is a lethal cancer with unknown molecular etiology and a high propensity to lymph node metastasis. Mostly due to its orphan status, MAC remains one of the least explored cancers that lacks cell lines and mouse models that could help translational and pre-clinical studies. Surgery with or without radiation remains the only treatment modality but poor overall survival (10-year, 44%) underscores the urgent need for mechanism-based therapies...
March 2017: Oral Oncology
https://www.readbyqxmd.com/read/28248250/advances-of-salivary-proteomics-in-oral-squamous-cell-carcinoma-oscc-detection-an-update
#5
REVIEW
Rabia Sannam Khan, Zohaib Khurshid, Shazia Akhbar, Syed Faraz Moin
Oral cancer refers to malignancies that have higher morbidity and mortality rates due to the late stage diagnosis and no early detection of a reliable diagnostic marker, while oral squamous cell carcinoma (OSCC) is amongst the world's top ten most common cancers. Diagnosis of cancer requires highly sensitive and specific diagnostic tools which can support untraceable hidden sites of OSCC, yet to be unleashed, for which plenty of biomarkers are identified; the most recommended biomarker detection medium for OSCC includes biological fluids, such as blood and saliva...
December 15, 2016: Proteomes
https://www.readbyqxmd.com/read/28236856/could-mucin-16-and-colony-stimulating-factor-2-receptor-beta-possible-graft-versus-host-disease-biomarkers-medical-hypotheses
#6
Milena Monteiro de Souza, Fabiana Martins de Paula, Ricardo Hsieh, Maria Cristina Martins Almeida Macedo, Marcelo Andreetta Corral, Thaís Borguezan Nunes, Fernanda De Paula, Silvia Vanessa Lourenço
Graft versus host disease (GVHD) occurs after bone marrow transplantation and is one of the most important causes of death worldwide. Reviews demonstrated GVHD patients with involvement of various tissues and organs, such as salivary glands. The diagnosis of acute GVHD has been the biopsies and the histopathologic evaluation of tissue from an involved organ. These procedures are invasive. Saliva proteins as possible biomarker for GVHD could facilitate the management and diagnosis accuracy. For support the proposed hypotheses, in this pilot study we collected whole saliva samples from patients with undergoing allogeneic hematopoietic cell transplantation (HCT) and from healthy subjects...
March 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28235782/development-of-a-multiplexed-lc-mrm-ms-method-for-evaluation-of-salivary-proteins-as-oral-cancer-biomarkers
#7
Yi-Ting Chen, Hsiao-Wei Chen, Chun-Feng Wu, Lichieh Julie Chu, Wei-Fang Chiang, Chih-Ching Wu, Jau-Song Yu, Cheng-Han Tsai, Kung-Hao Liang, Yu-Sun Chang, Maureen Wu, Wei-Ting Ou Yang
Multiple (selected) reaction monitoring (MRM/SRM) of peptides is a growing technology for target protein quantification because it is more robust, precise, accurate, high-throughput, and multiplex-capable than antibody-based techniques. The technique has been applied clinically to the large-scale quantification of multiple target proteins in different types of fluids. However, previous MRM-based studies have placed less focus on sample-preparation workflow and analytical performance in the precise quantification of proteins in saliva, a non-invasively sampled body fluid...
February 24, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28225606/effect-of-astringent-stimuli-on-salivary-protein-interactions-elucidated-by-complementary-proteomics-approaches
#8
Judith Delius, Guillaume Médard, Bernhard Kuster, Thomas Hofmann
The interaction of astringent substances with salivary proteins, which results in protein precipitation, is considered a key event in the molecular mechanism underlying the oral sensation of puckering astringency. As the chemical nature of orally active astringents is diverse and the knowledge of their interactions with salivary proteins rather fragmentary, human whole saliva samples were incubated with suprathreshold and isointensity solutions of the astringent polyphenol (-)-epigallocatechin gallate, the multivalent metal salt iron(III) sulfate, the amino-functionalized polysaccharide chitosan, and the basic protein lysozyme...
March 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28223859/histatin-peptides-pharmacological-functions-and-their-applications-in-dentistry
#9
REVIEW
Zohaib Khurshid, Shariq Najeeb, Maria Mali, Syed Faraz Moin, Syed Qasim Raza, Sana Zohaib, Farshid Sefat, Muhammad Sohail Zafar
There are many human oral antimicrobial peptides responsible for playing important roles including maintenance, repairing of oral tissues (hard or soft) and defense against oral microbes. In this review we have highlighted the biochemistry, physiology and proteomics of human oral histatin peptides, secreted from parotid and submandibular salivary glands in human. The significance of these peptides includes capability for ionic binding that can kill fungal Candida albicans. They have histidine rich amino acid sequences (7-12 family members; corresponding to residues 12-24, 13-24, 12-25, 13-25, 5-11, and 5-12, respectively) for Histatin-3...
January 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28198151/comparative-proteomic-analysis-of-the-influence-of-gender-and-acid-stimulation-on-normal-human-saliva-using-lc-ms-ms
#10
Xiaoping Xiao, Yaoran Liu, Zhengguang Guo, Xiaoyan Liu, Haidan Sun, Qian Li, Wei Sun
Human saliva is an important source for disease biomarker discovery. In this work, we investigate the influence of gender and acid stimulation on the normal human salivary proteome. Un-stimulated and acid-stimulated saliva samples from 5 males and 5 females were labeled with 4-plex iTRAQ and analyzed by 2-DLC MS/MS. A total of 1,770 proteins were identified, and 82 proteins in un-stimulated saliva were found to be gender-specific. These proteins were mainly associated with immune function, metabolism and inflammation...
February 15, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28178587/top-down-hplc-esi-ms-proteomic-analysis-of-saliva-of-edentulous-subjects-evidenced-high-levels-of-cystatin-a-cystatin-b-and-sprr3
#11
Barbara Manconi, Barbara Liori, Tiziana Cabras, Federica Iavarone, Armando Manni, Irene Messana, Massimo Castagnola, Alessandra Olianas
OBJECTIVE: This study aims to analyze the salivary peptidome/proteome of edentulous subject with respect to dentate control subjects. DESIGN: Unstimulated whole saliva, collected from 11 edentulous subjects (age 60-76 years) and 11 dentate age-matched control subjects, was immediately treated with 0.2% aqueous trifluoroacetic acid and the acidic soluble fraction analyzed by High Performace Liquid Chromatography-Mass Spectrometry. The relative abundance of the salivary peptides/proteins was determined by measuring the area of the High Performace Liquid Chromatography-Mass Spectrometry eXtracted Ion Current peaks which is linearly proportional to peptide/protein concentration under identical experimental conditions...
January 31, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28159752/studies-of-an-androgen-binding-protein-knockout-corroborate-a-role-for-salivary-abp-in-mouse-communication
#12
Amanda G Chung, Phillip M Belone, Barbora Vošlajerová Bímová, Robert M Karn, Christina M Laukaitis
The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and betagamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland cDNA libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands...
February 3, 2017: Genetics
https://www.readbyqxmd.com/read/28158262/uncovering-stem-cell-differentiation-factors-for-salivary-gland-regeneration-by-quantitative-analysis-of-differential-proteomes
#13
Yun-Jong Park, Jin Koh, Jin Teak Kwon, Yong-Seok Park, Lijun Yang, Seunghee Cha
Severe xerostomia (dry mouth) compromises the quality of life in patients with Sjögren's syndrome or radiation therapy for head and neck cancer. A clinical management of xerostomia is often unsatisfactory as most interventions are palliative with limited efficacy. Following up our previous study demonstrating that mouse BM-MSCs are capable of differentiating into salivary epithelial cells in a co-culture system, we further explored the molecular basis that governs the MSC reprogramming by utilizing high-throughput iTRAQ-2D-LC-MS/MS-based proteomics...
2017: PloS One
https://www.readbyqxmd.com/read/28122643/identification-of-potential-saliva-and-tear-biomarkers-in-primary-sj%C3%A3-gren-s-syndrome-utilising-the-extraction-of-extracellular-vesicles-and-proteomics-analysis
#14
Lara A Aqrawi, Hilde Kanli Galtung, Beate Vestad, Reidun Øvstebø, Bernd Thiede, Shermin Rusthen, Alix Young, Eduarda M Guerreiro, Tor Paaske Utheim, Xiangjun Chen, Øygunn Aass Utheim, Øyvind Palm, Janicke Liaaen Jensen
BACKGROUND: There is a long-lasting need for non-invasive, more accurate diagnostic techniques when evaluating primary Sjögren's syndrome (pSS) patients. Incorporation of additional diagnostics involving screening for disease-specific biomarkers in biological fluid is a promising concept that requires further investigation. In the current study we aimed to explore novel disease biomarkers in saliva and tears from pSS patients. METHODS: Liquid chromatography-mass spectrometry (LC-MS) was performed on stimulated whole saliva and tears from 27 pSS patients and 32 healthy controls, and salivary and tear proteomic biomarker profiles were generated...
January 25, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28055279/salivary-protein-changes-in-response-to-acute-stress-in-medical-residents-performing-advanced-clinical-simulations-a-pilot-proteomics-study
#15
Rachel K Marvin, Muncharie B Saepoo, Simiao Ye, Donald B White, Rong Liu, Kenneth Hensley, Paul Rega, Viviane Kazan, David R Giovannucci, Dragan Isailovic
CONTEXT: Quantitative changes of salivary proteins due to acute stress were detected. OBJECTIVE: To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations. MATERIALS AND METHODS: Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry...
January 25, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/28049606/an-insight-into-the-sialome-of-hyalomma-excavatum
#16
José M C Ribeiro, Mirko Slovák, Ivo M B Francischetti
Tick saliva contains hundreds or thousands of proteins that help blood feeding by impairing their hosts' hemostasis, inflammation and immunity. Salivary gland transcriptomes allow the disclosure of this pharmacologically active potion that consists of several multi-gene families, many of which are tick-specific. We here report the "de novo" assembly of ∼138 million reads deriving from a cDNA library from salivary glands of adult male and female Hyalomma excavatum leading to the public deposition of 5337 coding sequences to GenBank...
February 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28018380/functional-evaluation-of-proteins-in-watery-and-gel-saliva-of-aphids
#17
REVIEW
Aart J E van Bel, Torsten Will
Gel and watery saliva are regarded as key players in aphid-pIant interactions. The salivary composition seems to be influenced by the variable environment encountered by the stylet tip. Milieu sensing has been postulated to provide information needed for proper stylet navigation and for the required switches between gel and watery saliva secretion during stylet progress. Both the chemical and physical factors involved in sensing of the stylet's environment are discussed. To investigate the salivary proteome, proteins were collected from dissected gland extracts or artificial diets in a range of studies...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27978919/-current-advance-in-saliva-derived-exosome-study
#18
Y Zhao, L Y Yin, R R Bi, P Ma, S B Li
There is growing interest in the whole saliva as a diagnostic fluid because of the relatively simple and non-invasive collection. Research on salivary proteomic and genomic has greatly facilitated the diagnosis and therapy of oral diseases, also revealed the molecular mechanism of the diseases. Saliva-derived exosome as a new source, with various molecular constituents of their cells of origin, including proteins, mRNA and miRNA might serve as potential biomarker. This article reviews the biological properties of saliva exosome, its separation and other aspects associated with disease...
December 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27976689/the-proteomic-profile-of-whole-and-glandular-saliva-in-healthy-pain-free-subjects
#19
Hajer Jasim, Patrik Olausson, Britt Hedenberg-Magnusson, Malin Ernberg, Bijar Ghafouri
Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum to be able to reproducibly assess variability in states of health and disease. In the current study, the proteome profile in unstimulated and stimulated whole, parotid and sublingual saliva was investigated using two-dimensional gel electrophoresis...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27966577/salivary-and-pellicle-proteome-a-datamining-analysis
#20
Hardy Schweigel, Michael Wicht, Falk Schwendicke
We aimed to comprehensively compare two compartmented oral proteomes, the salivary and the dental pellicle proteome. Systematic review and datamining was used to obtain the physico-chemical, structural, functional and interactional properties of 1,515 salivary and 60 identified pellicle proteins. Salivary and pellicle proteins did not differ significantly in their aliphatic index, hydrophaty, instability index, or isoelectric point. Pellicle proteins were significantly more charged at low and high pH and were significantly smaller (10-20 kDa) than salivary proteins...
December 14, 2016: Scientific Reports
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