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Salivary proteomics

Sineepat Talungchit, Waranun Buajeeb, Chotima Lerdtripop, Rudee Surarit, Kongthawat Chairatvit, Sittiruk Roytrakul, Hiroaki Kobayashi, Yuichi Izumi, Siribang-On Piboonniyom Khovidhunkit
BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers...
March 13, 2018: BMC Oral Health
Richard Hemmi Valente, Fernanda Sakai, José Antonio Portes-Junior, Luciana Godoy Viana, Sylvia Mendes Carneiro, Jonas Perales, Norma Yamanouye
Despite numerous studies concerning morphology and venom production and secretion in the main venom gland (and some data on the accessory gland) of the venom glandular apparatus of Viperidae snakes, the primary duct has been overlooked. We characterized the primary duct of the Bothrops jararaca snake by morphological analysis, immunohistochemistry and proteomics. The duct has a pseudostratified epithelium with secretory columnar cells with vesicles of various electrondensities, as well as mitochondria-rich, dark, basal, and horizontal cells...
March 13, 2018: Toxins
Loren J Rivera-Vega, Bruce A Stanley, Anne Stanley, Gary W Felton
Insect saliva is one of the first secretions to come in contact with plants during feeding. The composition and role of caterpillar saliva has not been as thoroughly studied as that of sucking insects. This study focuses on characterizing the proteome of the cabbage looper (Trichoplusia ni) saliva using iTRAQ labeling and LC-MS/MS. We also measured how the saliva proteome changed when larvae were reared on different diets - cabbage, tomato, and an artificial pinto bean diet. We identified 254 proteins in the saliva out of which 63 were differentially expressed...
March 2, 2018: Journal of Insect Physiology
Wei Song, Vimal Kothari, Ana M Velly, Marisa Cressatti, Adrienne Liberman, Mervyn Gornitsky, Hyman M Schipper
BACKGROUND AND HYPOTHESIS: To date, there are no chemical analytes, including biochemical indices of oxidative stress, metabolites of α-synuclein protein, and differential protein expression patterns on proteomic profiling, for use in clinics as a diagnostic biomarker of idiopathic PD. OBJECTIVES: Heme oxygenase-1 has been implicated in the pathogenesis of PD. The objective of this study is to ascertain whether salivary heme oxygenase-1 may serve as a biomarker for early idiopathic PD...
February 28, 2018: Movement Disorders: Official Journal of the Movement Disorder Society
Mythily Srinivasan, Melinda L Meadows, Lisa Maxwell
Type 2 diabetes mellitus (T2DM) is emerging as a metabolic epidemic worldwide. Pathologically, dysregulation of many biological pathways precedes hyperglycemia and the clinical diagnosis of T2DM. Changing trajectories along the process of T2DM development necessitates frequent measurement of biomarkers for early identification of at-risk individuals and successful prevention. Increase in circulating inflammatory adipokines has been suggested as predictive of T2DM. Human saliva is an easily accessible biospecimen amenable for painless frequent collection and possesses nearly 50% of serum proteome...
2018: Biochemistry Research International
Lei Liu, Yi-Song Liu, Guo-Hua Liu, Tian-Yin Cheng
BACKGROUND: Ticks and tick-borne diseases are of major public health concern. Currently, development of vaccines against ticks is considered crucial for their control. A critical step in this process is the screening of viable antigens. Faeces are byproducts of digestion and blood meal utilization, and partly reflect the vitality and vector potential of ticks. However, an integrated analysis of proteins in tick faeces is lacking. The present study explored the protein components in the faeces of the tick Haemaphysalis flava, by liquid chromatography-tandem mass spectrometry (LC/MS-MS) to identify potential protein antigens for vaccine development against ticks...
February 8, 2018: Parasites & Vectors
Yan Sun, Chunhui Huo, Zhi Qiao, Zhi Shang, Asad Uzzaman, Sha Liu, Xiaoteng Jiang, Liu-Yin Fan, Liyun Ji, Xin Guan, Cheng-Xi Cao, Hua Xiao
Extracellular vesicles (EVs) are cell-derived microparticles present in most body fluids, mainly including microvesicles and exosomes. EV-harbored proteins have emerged as novel biomarkers for the diagnosis and prediction of different cancers. We successfully isolated microvesicles and exosomes from human saliva, which were further characterized comprehensively. Salivary EV protein profiling in normal subjects and lung cancer patients was systematically compared through utilizing LC-MS/MS-based label-free quantification...
March 2, 2018: Journal of Proteome Research
Milena M Souza, Giovanna P Florezi, Marcello M S Nico, Fernanda de Paula, Fabiana M de Paula, Silvia V Lourenço
OBJECTIVES: Oral lichen planus is a chronic, T-cell-mediated, inflammatory disease that affects the oral cavity. The oral lichen planus pathogenesis is still unclear, however, the main evidence is that the mechanisms of activation of different T lymphocytes pathways induce apoptosis with an increase of Th1 and Th17 subtypes cells, triggered by the release of cytokines; This study analysed saliva proteomics to identify protein markers that might be involved in the pathogenesis and development of the disease...
January 30, 2018: Oral Diseases
Lei Liu, Tian-Yin Cheng, Xiao-Ming He
Scant information is available regarding the proteins involved in blood meal processing in ticks. Here, we aimed to highlight the midgut proteins involved in preventing blood meal coagulation, and in facilitating intracellular digestion in the tick Haemaphysalis flava. Proteins were extracted from the midgut contents of fully engorged and partially engorged ticks. We used liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify 131 unique peptides, and 102 proteins. Of these, 15 proteins, each with at least two unique peptides, were recognized with high confidence...
January 19, 2018: Ticks and Tick-borne Diseases
Kun Wang, Yufei Wang, Xiuqing Wang, Qian Ren, Sili Han, Longjiang Ding, Zhongcheng Li, Xuedong Zhou, Wei Li, Linglin Zhang
BACKGROUND: Dental caries is a major worldwide oral disease afflicting a large proportion of children. As an important host factor of caries susceptibility, saliva plays a significant role in the occurrence and development of caries. The aim of the present study was to characterize the healthy and cariogenic salivary proteome and determine the changes in salivary protein expression of children with varying degrees of active caries, also to establish salivary proteome profiles with a potential therapeutic use against dental caries...
January 19, 2018: Journal of Translational Medicine
N G Shashikumar, R K Baithalu, S Bathla, S A Ali, P Rawat, A Kumaresan, S Kumar, B R Maharana, G Singh, D S Puneeth Kumar, S K Singh, S S Lathwal, L Jaiswal, T K Mohanty, A K Mohanty
Accurate and efficient detection of estrus is one of the major constraints for exploitation of the production potential of buffalo owing to its poor manifestation of estrus signs, seasonal differences in expression and higher incidences of silent estrus (29%). The current study focused on identification of estrus specific candidate proteins in saliva of buffaloes. Estrus was detected based on behavioral signs in response to the teaser and changes in reproductive organs and confirmed by per-rectal examination, trans-rectal USG of reproductive organs, cervico-vaginal mucus characteristics and blood serum progesterone estimation...
January 9, 2018: Theriogenology
Sheila M F Torres, Eva Furrow, Clarissa P Souza, Jennifer L Granick, Ebbing P de Jong, Timothy J Griffin, Xiong Wang
OBJECTIVE: To provide an in-depth catalog of the salivary proteome and endogenous peptidome of healthy dogs, evaluate proteins and peptides with antimicrobial properties, and compare the most common salivary proteins and peptides between different breed phylogeny groups. METHODS: 36 healthy dogs without evidence of periodontal disease representing four breed phylogeny groups, based upon single nucleotide polymorphism haplotypes (ancient, herding/sighthound, and two miscellaneous groups)...
2018: PloS One
Sabah Pasha, Taichi Inui, Iain Chapple, Stephen Harris, Lucy Holcombe, Melissa M Grant
Saliva is a complex multifunctional fluid that bathes the oral cavity to assist in soft and hard tissue maintenance, lubrication, buffering, defence against microbes and initiating digestion of foods. It has been extensively characterised in humans but its protein composition in dogs remains poorly characterised, yet could explain (patho) physiological differences between individuals, breeds and with humans. This pilot discovery study aimed to characterise canine saliva from two breeds, Labrador retrievers and Beagles, and to compare this with human saliva using quantitative mass spectrometry...
January 12, 2018: Proteomics
Noha Ayman Ghallab
OBJECTIVES: The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival crevicular fluid (GCF) and saliva. METHODS: A literature search was performed using the Cochrane library and PubMed databases from 2000 to January 2017...
December 23, 2017: Archives of Oral Biology
Paula Beatriz Santiago, Carla Nunes de Araújo, Sébastien Charneau, Izabela Marques Dourado Bastos, Teresa Cristina F Assumpção, Rayner Myr Lauterjung Queiroz, Yanna Reis Praça, Thuany de Moura Cordeiro, Carlos Henrique Saraiva Garcia, Ionizete Garcia da Silva, Tainá Raiol, Flávia Nader Motta, João Victor de Araújo Oliveira, Marcelo Valle de Sousa, José Marcos C Ribeiro, Jaime Martins de Santana
Triatoma dimidiata, a Chagas disease vector widely distributed along Central America, has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas fed human blood. Exploring the salivary compounds that overcome host haemostatic and immune responses is of great scientific interest. Here, we provide a deeper insight into its salivary gland molecules. We used high-throughput RNA sequencing to examine in depth the T. dimidiata salivary gland transcriptome...
December 27, 2017: Journal of Proteomics
James Nyagwange, Edwin Tijhaar, Nicola Ternette, Fredrick Mobegi, Kyle Tretina, Joana C Silva, Roger Pelle, Vishvanath Nene
East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T...
December 16, 2017: International Journal for Parasitology
Md Tajmul, Farhat Parween, Lata Singh, Sandeep R Mathur, J B Sharma, Sunesh Kumar, D N Sharma, Savita Yadav
Ovarian cancer (OC) is one of the most lethal cancers among all gynecological malignancies. An effective and non-invasive screening approach is needed urgently to reduce high mortality rate. The purpose of this study was to identify the salivary protein signatures (SPS) for non-invasive detection of ovarian cancer. Differentially expressed SPS were identified by fluorescence-based 2D-DIGE coupled with MALDI/TOF-MS. The expression levels of three differential proteins (Lipocalin-2, indoleamine-2, 3-dioxygenase1 (IDO1) and S100A8) were validated using western blotting and ELISA...
March 2018: International Journal of Biological Macromolecules
Eliane Esteves, Sandra R Maruyama, Rebeca Kawahara, André Fujita, Larissa A Martins, Adne A Righi, Francisco B Costa, Giuseppe Palmisano, Marcelo B Labruna, Anderson Sá-Nunes, José M C Ribeiro, Andréa C Fogaça
Ticks are obligate blood feeding ectoparasites that transmit a wide variety of pathogenic microorganisms to their vertebrate hosts. Amblyomma sculptum is vector of Rickettsia rickettsii , the causative agent of Rocky Mountain spotted fever (RMSF), the most lethal rickettsiosis that affects humans. It is known that the transmission of pathogens by ticks is mainly associated with the physiology of the feeding process. Pathogens that are acquired with the blood meal must first colonize the tick gut and later the salivary glands (SG) in order to be transmitted during a subsequent blood feeding via saliva...
2017: Frontiers in Cellular and Infection Microbiology
Ana Gutiérrez, José Joaquín Cerón, Ebrahim Razzazi-Fazeli, Sarah Schlosser, Fernando Tecles
BACKGROUND: The influence of two different sample treatments comprising the enrichment of glycoproteins by boronic acid and dynamic range compression by hexapeptide libraries, on the detection of stress markers in saliva of pigs was evaluated in this study. For this purpose, saliva samples collected before and after the application of an acute stress model consisting of nasal restraining in pigs were processed without any treatment and with the two different treatments mentioned above...
December 4, 2017: BMC Veterinary Research
Chukkris Heawchaiyaphum, Chamsai Pientong, Pensiri Phusingha, Patravoot Vatanasapt, Supannee Promthet, Jureerut Daduang, Watchareporn Teeramatwanich, Bunkerd Kongyingyoes, Jureeporn Chuerduangphui, Tipaya Ekalaksananan
No effective screening method is available for oral squamous cell carcinoma (OSCC) that is recognized to influence by environmental factors as well as human papillomavirus (HPV) and Epstein-Barr virus (EBV). Therefore, we sought to identify salivary biomarkers for screening of OSCC with or without HPV and/or EBV infection. Saliva, lesion and oral exfoliated cells were collected from OSCC patients and cancer-free controls (CFCs) and grouped depending on their HPV- and EBV-infection status. Salivary protein was precipitated and subjected to 2-dimensional gel electrophoresis...
February 20, 2018: Journal of Proteomics
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