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Salivary proteomics

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https://www.readbyqxmd.com/read/29755368/saliva-proteomics-analysis-offers-insights-on-type-1-diabetes-pathology-in-a-pediatric-population
#1
Eftychia Pappa, Heleni Vastardis, George Mermelekas, Andriani Gerasimidi-Vazeou, Jerome Zoidakis, Konstantinos Vougas
The composition of the salivary proteome is affected by pathological conditions. We analyzed by high resolution mass spectrometry approaches saliva samples collected from children and adolescents with type 1 diabetes and healthy controls. The list of more than 2000 high confidence protein identifications constitutes a comprehensive characterization of the salivary proteome. Patients with good glycemic regulation and healthy individuals have comparable proteomic profiles. In contrast, a significant number of differentially expressed proteins were identified in the saliva of patients with poor glycemic regulation compared to patients with good glycemic control and healthy children...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29750386/saliva-in-the-omics-era-a-promising-tool-in-paediatrics
#2
Eftychia Pappa, Eleni Kousvelari, Heleni Vastardis
In vulnerable populations, such as infants and children, saliva makes the perfect diagnostic medium because of its non-invasive collection, easy handling and storage of samples. Its unique biomarker profiles help tremendously in the diagnosis of many diseases and conditions. In fact, saliva genomics, proteomics, transcriptomics, metabolomics and microbiome-based discoveries have led to complementary and powerful diagnostic information. In children and neonates, saliva is the preferred medium not only for diagnosis of caries and aggressive periodontitis but also for a number of systemic conditions, metabolic diseases, cognitive functions, stress assessment and evaluation of immunological and inflammatory responses to vaccination...
May 11, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29727440/proteomic-analysis-of-watery-saliva-secreted-by-white-backed-planthopper-sogatella-furcifera
#3
Yu-Tong Miao, Yao Deng, Hao-Kang Jia, Yu-Di Liu, Mao-Lin Hou
The white-backed planthopper, Sogatella furcifera, is a phloem sap feeder that secretes watery and gelling saliva during feeding. In this study, we identified the major proteins in watery saliva of S. furcifera by shotgun LC-MS/MS analysis combined with transcriptomic analysis. A total of 161 proteins were identified, which were divided into 8 function categories, including enzymes, transporter, calcium ion binding protein, salivary sheath protein, cytoskeleton protein, DNA-, RNA-, and protein-binding or regulating proteins, other non-enzyme proteins and unknown proteins...
2018: PloS One
https://www.readbyqxmd.com/read/29721505/salivary-biomarkers-in-systemic-sclerosis-disease
#4
REVIEW
Zeineb Zian, Joaira Bakkach, Amina Barakat, Naima Ghailani Nourouti, Mohcine Bennani Mechita
Scleroderma or systemic sclerosis (SSc) is frequently detected at an advanced stage due to diagnosis difficulties. Salivary biomarkers, if existing, could be used for predictive diagnosis of this disease. Human saliva contains a large number of proteins that can be used for diagnosis and are of great potential in clinical research. The use of proteomic analysis to characterize whole saliva (WS) in SSc has gained an increasing attention in the last years and the identification of salivary proteins specific for SSc could lead to early diagnosis or new therapeutic targets...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29652888/sialome-diversity-of-ticks-revealed-by-rnaseq-of-single-tick-salivary-glands
#5
Jan Perner, Sára Kropáčková, Petr Kopáček, José M C Ribeiro
Ticks salivate while feeding on their hosts. Saliva helps blood feeding through host anti-hemostatic and immunomodulatory components. Previous transcriptomic and proteomic studies revealed the complexity of tick saliva, comprising hundreds of polypeptides grouped in several multi-genic families such as lipocalins, Kunitz-domain containing peptides, metalloproteases, basic tail secreted proteins, and several other families uniquely found in ticks. These studies also revealed that the composition of saliva changes with time; expression of transcripts from the same family wax and wane as a function of feeding time...
April 13, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29608211/annexin-1-as-a-salivary-biomarker-for-gingivitis-during-pregnancy
#6
Manar N Hassan, Georgios N Belibasakis, Pinar Gumus, Veli Özgen Öztürk, Gulnur Emingil, Nagihan Bostanci
BACKGROUND: It is well-established that there is a higher susceptibility to gingival inflammation during pregnancy. Annexin-1 (ANXA1) is an anti-inflammatory protein, which has been identified in gingival tissue exudates by discovery proteomics. The aim of this cross-sectional case-control study was to investigate the levels and association of ANXA1 and pro-inflammatory mediator Interleukin (IL)-1β in the saliva of pregnant and non-pregnant women. METHODS: Whole unstimulated saliva from 69 non-pregnant and 78 pregnant women was collected prior to measurement of probing pocket depth, clinical attachment level, bleeding on probing and plaque...
April 2, 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29570734/characterization-of-the-adult-aedes-aegypti-early-midgut-peritrophic-matrix-proteome-using-lc-ms
#7
Shavonn R Whiten, W Keith Ray, Richard F Helm, Zach N Adelman
The Aedes aegypti mosquito is the principal vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus. These arboviruses are transmitted during adult female mosquito bloodfeeding. While these viruses must transverse the midgut to replicate, the blood meal must also reach the midgut to be digested, absorbed, or excreted, as aggregation of blood meal metabolites can be toxic to the female mosquito midgut. The midgut peritrophic matrix (PM), a semipermeable extracellular layer comprised of chitin fibrils, glycoproteins, and proteoglycans, is one such mechanism of protection for the mosquito midgut...
2018: PloS One
https://www.readbyqxmd.com/read/29558726/detection-of-salivary-protein-biomarkers-of-saliva-secretion-disorder-in-a-primary-sj%C3%A3-gren-syndrome-murine-model
#8
Peisheng Liang, Wangyong Zhu, Tianjun Lan, Qian Tao
Saliva secretion disorder is one of the most common symptoms in primary Sjögren syndrome (pSS). Salivary biomarkers related to saliva secretion disorder were identified in a pSS murine model, NOD/ShiLtJ mouse, using differential proteomic analysis. Candidate biomarkers were screened with Kyoto Encyclopedia of Genes and Genomes pathway analysis and validated in saliva and salivary glands by western blotting and immunohistochemistry (IHC). Biological functions were detected using ingenuity pathway analysis (IPA)...
March 12, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29548478/quantitative-proteomics-and-swath-ms-to-elucidate-peri-receptor-mechanisms-in-human-salt-taste-sensitivity
#9
Theresa Stolle, Freya Grondinger, Andreas Dunkel, Thomas Hofmann
Recently, studies on human salt taste sensitivity demonstrated that sodium chloride (NaCl) sensitive and non-sensitive subjects differed in their salivary proteome and, in particular, in endopeptidase activity. In order to investigate individual's NaCl sensitivity and the role of endoprotease activity in salt taste perception, 20 panellists were classified according to NaCl sensitivity and saliva samples collected. A targeted protein quantitation by means of selected-reaction-monitoring (SRM) mass spectrometry and stable-isotope incorporation revealed the joint abundance of lysozyme C and lipocalin-1 to be indicative for non-sensitive subjects...
July 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29534707/putative-salivary-protein-biomarkers-for-the-diagnosis-of-oral-lichen-planus-a-case-control-study
#10
Sineepat Talungchit, Waranun Buajeeb, Chotima Lerdtripop, Rudee Surarit, Kongthawat Chairatvit, Sittiruk Roytrakul, Hiroaki Kobayashi, Yuichi Izumi, Siribang-On Piboonniyom Khovidhunkit
BACKGROUND: Salivary protein biomarkers for screening and diagnosis of oral lichen planus (OLP) are not well-defined. The objective of this study was to identify putative protein biomarkers for OLP using proteomic approaches. METHODS: Pooled unstimulated whole saliva was collected from five OLP patients and five healthy control participants. Saliva samples were then subjected to two-dimensional gel electrophoresis, followed by mass spectrometry to identify putative protein biomarkers...
March 13, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29533989/the-primary-duct-of-bothrops-jararaca-glandular-apparatus-secretes-toxins
#11
Richard Hemmi Valente, Fernanda Sakai, José Antonio Portes-Junior, Luciana Godoy Viana, Sylvia Mendes Carneiro, Jonas Perales, Norma Yamanouye
Despite numerous studies concerning morphology and venom production and secretion in the main venom gland (and some data on the accessory gland) of the venom glandular apparatus of Viperidae snakes, the primary duct has been overlooked. We characterized the primary duct of the Bothrops jararaca snake by morphological analysis, immunohistochemistry and proteomics. The duct has a pseudostratified epithelium with secretory columnar cells with vesicles of various electrondensities, as well as mitochondria-rich, dark, basal, and horizontal cells...
March 13, 2018: Toxins
https://www.readbyqxmd.com/read/29505761/proteomic-analysis-of-labial-saliva-of-the-generalist-cabbage-looper-trichoplusia-ni-and-its-role-in-interactions-with-host-plants
#12
Loren J Rivera-Vega, Bruce A Stanley, Anne Stanley, Gary W Felton
Insect saliva is one of the first secretions to come in contact with plants during feeding. The composition and role of caterpillar saliva has not been as thoroughly studied as that of sucking insects. This study focuses on characterizing the proteome of the cabbage looper (Trichoplusia ni) saliva using iTRAQ labeling and LC-MS/MS. We also measured how the saliva proteome changed when larvae were reared on different diets - cabbage, tomato, and an artificial pinto bean diet. We identified 254 proteins in the saliva out of which 63 were differentially expressed...
March 2, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29488275/evaluation-of-salivary-heme-oxygenase-1-as-a-potential-biomarker-of-early-parkinson-s-disease
#13
Wei Song, Vimal Kothari, Ana M Velly, Marisa Cressatti, Adrienne Liberman, Mervyn Gornitsky, Hyman M Schipper
BACKGROUND AND HYPOTHESIS: To date, there are no chemical analytes, including biochemical indices of oxidative stress, metabolites of α-synuclein protein, and differential protein expression patterns on proteomic profiling, for use in clinics as a diagnostic biomarker of idiopathic PD. OBJECTIVES: Heme oxygenase-1 has been implicated in the pathogenesis of PD. The objective of this study is to ascertain whether salivary heme oxygenase-1 may serve as a biomarker for early idiopathic PD...
April 2018: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29487749/assessment-of-salivary-adipokines-resistin-visfatin-and-ghrelin-as-type-2-diabetes-mellitus-biomarkers
#14
Mythily Srinivasan, Melinda L Meadows, Lisa Maxwell
Type 2 diabetes mellitus (T2DM) is emerging as a metabolic epidemic worldwide. Pathologically, dysregulation of many biological pathways precedes hyperglycemia and the clinical diagnosis of T2DM. Changing trajectories along the process of T2DM development necessitates frequent measurement of biomarkers for early identification of at-risk individuals and successful prevention. Increase in circulating inflammatory adipokines has been suggested as predictive of T2DM. Human saliva is an easily accessible biospecimen amenable for painless frequent collection and possesses nearly 50% of serum proteome...
2018: Biochemistry Research International
https://www.readbyqxmd.com/read/29422072/proteomics-analysis-of-faecal-proteins-in-the-tick-haemaphysalis-flava
#15
Lei Liu, Yi-Song Liu, Guo-Hua Liu, Tian-Yin Cheng
BACKGROUND: Ticks and tick-borne diseases are of major public health concern. Currently, development of vaccines against ticks is considered crucial for their control. A critical step in this process is the screening of viable antigens. Faeces are byproducts of digestion and blood meal utilization, and partly reflect the vitality and vector potential of ticks. However, an integrated analysis of proteins in tick faeces is lacking. The present study explored the protein components in the faeces of the tick Haemaphysalis flava, by liquid chromatography-tandem mass spectrometry (LC/MS-MS) to identify potential protein antigens for vaccine development against ticks...
February 8, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29397740/comparative-proteomic-analysis-of-exosomes-and-microvesicles-in-human-saliva-for-lung-cancer
#16
Yan Sun, Chunhui Huo, Zhi Qiao, Zhi Shang, Asad Uzzaman, Sha Liu, Xiaoteng Jiang, Liu-Yin Fan, Liyun Ji, Xin Guan, Cheng-Xi Cao, Hua Xiao
Extracellular vesicles (EVs) are cell-derived microparticles present in most body fluids, mainly including microvesicles and exosomes. EV-harbored proteins have emerged as novel biomarkers for the diagnosis and prediction of different cancers. We successfully isolated microvesicles and exosomes from human saliva, which were further characterized comprehensively. Salivary EV protein profiling in normal subjects and lung cancer patients was systematically compared through utilizing LC-MS/MS-based label-free quantification...
March 2, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29383810/salivary-proteomics-in-lichen-planus-a-relationship-with-pathogenesis
#17
M M Souza, G P Florezi, Mms Nico, F de Paula, F M Paula, S V Lourenço
OBJECTIVES: Oral lichen planus is a chronic, T-cell-mediated, inflammatory disease that affects the oral cavity. The oral lichen planus pathogenesis is still unclear, however, the main evidence is that the mechanisms of activation of different T lymphocyte pathway induce apoptosis with an increase in Th1 and Th17 subtypes cells, triggered by the release of cytokines. This study analysed saliva proteomics to identify protein markers that might be involved in the pathogenesis and development of the disease...
January 30, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29371124/proteomic-profiling-of-the-midgut-contents-of-haemaphysalis-flava
#18
Lei Liu, Tian-Yin Cheng, Xiao-Ming He
Scant information is available regarding the proteins involved in blood meal processing in ticks. Here, we aimed to highlight the midgut proteins involved in preventing blood meal coagulation, and in facilitating intracellular digestion in the tick Haemaphysalis flava. Proteins were extracted from the midgut contents of fully engorged and partially engorged ticks. We used liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify 131 unique peptides, and 102 proteins. Of these, 15 proteins, each with at least two unique peptides, were recognized with high confidence...
March 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29351798/comparative-salivary-proteomics-analysis-of-children-with-and-without-dental-caries-using-the-itraq-mrm-approach
#19
Kun Wang, Yufei Wang, Xiuqing Wang, Qian Ren, Sili Han, Longjiang Ding, Zhongcheng Li, Xuedong Zhou, Wei Li, Linglin Zhang
BACKGROUND: Dental caries is a major worldwide oral disease afflicting a large proportion of children. As an important host factor of caries susceptibility, saliva plays a significant role in the occurrence and development of caries. The aim of the present study was to characterize the healthy and cariogenic salivary proteome and determine the changes in salivary protein expression of children with varying degrees of active caries, also to establish salivary proteome profiles with a potential therapeutic use against dental caries...
January 19, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29334660/global-proteomic-analysis-of-water-buffalo-bubalus-bubalis-saliva-at-different-stages-of-estrous-cycle-using-high-throughput-mass-spectrometry
#20
N G Shashikumar, R K Baithalu, S Bathla, S A Ali, P Rawat, A Kumaresan, S Kumar, B R Maharana, G Singh, D S Puneeth Kumar, S K Singh, S S Lathwal, L Jaiswal, T K Mohanty, A K Mohanty
Accurate and efficient detection of estrus is one of the major constraints for exploitation of the production potential of buffalo owing to its poor manifestation of estrus signs, seasonal differences in expression and higher incidences of silent estrus (29%). The current study focused on identification of estrus specific candidate proteins in saliva of buffaloes. Estrus was detected based on behavioral signs in response to the teaser and changes in reproductive organs and confirmed by per-rectal examination, trans-rectal USG of reproductive organs, cervico-vaginal mucus characteristics and blood serum progesterone estimation...
April 1, 2018: Theriogenology
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