keyword
MENU ▼
Read by QxMD icon Read
search

Salivary proteomics

keyword
https://www.readbyqxmd.com/read/28198151/comparative-proteomic-analysis-of-the-influence-of-gender-and-acid-stimulation-on-normal-human-saliva-using-lc-ms-ms
#1
Xiaoping Xiao, Yaoran Liu, Zhengguang Guo, Xiaoyan Liu, Haidan Sun, Qian Li, Wei Sun
Human saliva is an important source for disease biomarker discovery. In this work, we investigate the influence of gender and acid stimulation on the normal human salivary proteome. Un-stimulated and acid-stimulated saliva samples from 5 males and 5 females were labeled with 4-plex iTRAQ and analyzed by 2-DLC MS/MS. A total of 1,770 proteins were identified, and 82 proteins in un-stimulated saliva were found to be gender-specific. These proteins were mainly associated with immune function, metabolism and inflammation...
February 15, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28178587/top-down-hplc-esi-ms-proteomic-analysis-of-saliva-of-edentulous-subjects-evidenced-high-levels-of-cystatin-a-cystatin-b-and-sprr3
#2
Barbara Manconi, Barbara Liori, Tiziana Cabras, Federica Iavarone, Armando Manni, Irene Messana, Massimo Castagnola, Alessandra Olianas
OBJECTIVE: This study aims to analyze the salivary peptidome/proteome of edentulous subject with respect to dentate control subjects. DESIGN: Unstimulated whole saliva, collected from 11 edentulous subjects (age 60-76 years) and 11 dentate age-matched control subjects, was immediately treated with 0.2% aqueous trifluoroacetic acid and the acidic soluble fraction analyzed by High Performace Liquid Chromatography-Mass Spectrometry. The relative abundance of the salivary peptides/proteins was determined by measuring the area of the High Performace Liquid Chromatography-Mass Spectrometry eXtracted Ion Current peaks which is linearly proportional to peptide/protein concentration under identical experimental conditions...
January 31, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28159752/studies-of-an-androgen-binding-protein-knockout-corroborate-a-role-for-salivary-abp-in-mouse-communication
#3
Amanda G Chung, Phillip M Belone, Barbora Vošlajerová Bímová, Robert M Karn, Christina M Laukaitis
The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and betagamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland cDNA libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands...
February 3, 2017: Genetics
https://www.readbyqxmd.com/read/28158262/uncovering-stem-cell-differentiation-factors-for-salivary-gland-regeneration-by-quantitative-analysis-of-differential-proteomes
#4
Yun-Jong Park, Jin Koh, Jin Teak Kwon, Yong-Seok Park, Lijun Yang, Seunghee Cha
Severe xerostomia (dry mouth) compromises the quality of life in patients with Sjögren's syndrome or radiation therapy for head and neck cancer. A clinical management of xerostomia is often unsatisfactory as most interventions are palliative with limited efficacy. Following up our previous study demonstrating that mouse BM-MSCs are capable of differentiating into salivary epithelial cells in a co-culture system, we further explored the molecular basis that governs the MSC reprogramming by utilizing high-throughput iTRAQ-2D-LC-MS/MS-based proteomics...
2017: PloS One
https://www.readbyqxmd.com/read/28122643/identification-of-potential-saliva-and-tear-biomarkers-in-primary-sj%C3%A3-gren-s-syndrome-utilising-the-extraction-of-extracellular-vesicles-and-proteomics-analysis
#5
Lara A Aqrawi, Hilde Kanli Galtung, Beate Vestad, Reidun Øvstebø, Bernd Thiede, Shermin Rusthen, Alix Young, Eduarda M Guerreiro, Tor Paaske Utheim, Xiangjun Chen, Øygunn Aass Utheim, Øyvind Palm, Janicke Liaaen Jensen
BACKGROUND: There is a long-lasting need for non-invasive, more accurate diagnostic techniques when evaluating primary Sjögren's syndrome (pSS) patients. Incorporation of additional diagnostics involving screening for disease-specific biomarkers in biological fluid is a promising concept that requires further investigation. In the current study we aimed to explore novel disease biomarkers in saliva and tears from pSS patients. METHODS: Liquid chromatography-mass spectrometry (LC-MS) was performed on stimulated whole saliva and tears from 27 pSS patients and 32 healthy controls, and salivary and tear proteomic biomarker profiles were generated...
January 25, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28055279/salivary-protein-changes-in-response-to-acute-stress-in-medical-residents-performing-advanced-clinical-simulations-a-pilot-proteomics-study
#6
Rachel K Marvin, Muncharie B Saepoo, Simiao Ye, Donald B White, Rong Liu, Kenneth Hensley, Paul Rega, Viviane Kazan, David R Giovannucci, Dragan Isailovic
CONTEXT: Quantitative changes of salivary proteins due to acute stress were detected. OBJECTIVE: To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations. MATERIALS AND METHODS: Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by SDS-PAGE, identified by MS, and relatively quantified by densitometry. RESULTS: Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (<10 kDa) SDS-PAGE bands exhibited changes after stress...
January 5, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/28049606/an-insight-into-the-sialome-of-hyalomma-excavatum
#7
José M C Ribeiro, Mirko Slovák, Ivo M B Francischetti
Tick saliva contains hundreds or thousands of proteins that help blood feeding by impairing their hosts' hemostasis, inflammation and immunity. Salivary gland transcriptomes allow the disclosure of this pharmacologically active potion that consists of several multi-gene families, many of which are tick-specific. We here report the "de novo" assembly of ∼138 million reads deriving from a cDNA library from salivary glands of adult male and female Hyalomma excavatum leading to the public deposition of 5337 coding sequences to GenBank...
February 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28018380/functional-evaluation-of-proteins-in-watery-and-gel-saliva-of-aphids
#8
REVIEW
Aart J E van Bel, Torsten Will
Gel and watery saliva are regarded as key players in aphid-pIant interactions. The salivary composition seems to be influenced by the variable environment encountered by the stylet tip. Milieu sensing has been postulated to provide information needed for proper stylet navigation and for the required switches between gel and watery saliva secretion during stylet progress. Both the chemical and physical factors involved in sensing of the stylet's environment are discussed. To investigate the salivary proteome, proteins were collected from dissected gland extracts or artificial diets in a range of studies...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27978919/-current-advance-in-saliva-derived-exosome-study
#9
Y Zhao, L Y Yin, R R Bi, P Ma, S B Li
There is growing interest in the whole saliva as a diagnostic fluid because of the relatively simple and non-invasive collection. Research on salivary proteomic and genomic has greatly facilitated the diagnosis and therapy of oral diseases, also revealed the molecular mechanism of the diseases. Saliva-derived exosome as a new source, with various molecular constituents of their cells of origin, including proteins, mRNA and miRNA might serve as potential biomarker. This article reviews the biological properties of saliva exosome, its separation and other aspects associated with disease...
December 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27976689/the-proteomic-profile-of-whole-and-glandular-saliva-in-healthy-pain-free-subjects
#10
Hajer Jasim, Patrik Olausson, Britt Hedenberg-Magnusson, Malin Ernberg, Bijar Ghafouri
Determination of the variability in the salivary proteome is a prerequisite for the development of saliva as a diagnostic and prognostic tool in particular physiological states. In this context, it is important that technical variability induced by sample collection and processing is kept at minimum to be able to reproducibly assess variability in states of health and disease. In the current study, the proteome profile in unstimulated and stimulated whole, parotid and sublingual saliva was investigated using two-dimensional gel electrophoresis...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27966577/salivary-and-pellicle-proteome-a-datamining-analysis
#11
Hardy Schweigel, Michael Wicht, Falk Schwendicke
We aimed to comprehensively compare two compartmented oral proteomes, the salivary and the dental pellicle proteome. Systematic review and datamining was used to obtain the physico-chemical, structural, functional and interactional properties of 1,515 salivary and 60 identified pellicle proteins. Salivary and pellicle proteins did not differ significantly in their aliphatic index, hydrophaty, instability index, or isoelectric point. Pellicle proteins were significantly more charged at low and high pH and were significantly smaller (10-20 kDa) than salivary proteins...
December 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27943336/verification-of-protein-biomarkers-for-the-identification-of-biological-stains-by-quadrupole-time-of-flight-mass-spectrometry
#12
Kevin M Legg, Roger Powell, Nichole Reisdorph, Rick Reisdorph, Phillip B Danielson
Advances in proteomics technology over the past decade offer forensic serologists a greatly improved opportunity to accurately characterize the tissue source from which a DNA has been developed. Such information can provide critical context to evidence and can help to prioritize downstream DNA analysis. Previous proteome studies compiled panels of "candidate biomarkers" specific to each of six body fluids (i.e. peripheral blood, menstrual fluid, vaginal fluid, seminal fluid, urine and saliva). Here, a multiplex Quadrupole Time-of-flight assay has been developed in order to verify the tissue/body fluid specificity the 23 protein biomarkers that comprise these panels and the consistency with which they can be detected across a samples population of fifty humans...
December 12, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27943101/salivary-biomarkers-in-cancer-detection
#13
REVIEW
Xiaoqian Wang, Karolina Elżbieta Kaczor-Urbanowicz, David T W Wong
Cancer is the second most common cause of death in the USA. Its symptoms are often not specific and absent, until the tumors have already metastasized. Therefore, there is an urgent demand for developing rapid, highly accurate and noninvasive tools for cancer screening, early detection, diagnostics, staging and prognostics. Saliva as a multi-constituent oral fluid comprises secretions from the major and minor salivary glands, extensively supplied by blood. Molecules such as DNAs, RNAs, proteins, metabolites, and microbiota, present in blood, could be also found in saliva...
January 2017: Medical Oncology
https://www.readbyqxmd.com/read/27903834/saliva-diagnostics-current-views-and-directions
#14
Karolina Elżbieta Kaczor-Urbanowicz, Carmen Martin Carreras-Presas, Katri Aro, Michael Tu, Franklin Garcia-Godoy, David Tw Wong
In this review, we provide an update on the current and future applications of saliva for diagnostic purposes. There are many advantages of using saliva as a biofluid. Its collection is fast, easy, inexpensive, and non-invasive. In addition, saliva, as a "mirror of the body," can reflect the physiological and pathological state of the body. Therefore, it serves as a diagnostic and monitoring tool in many fields of science such as medicine, dentistry, and pharmacotherapy. Introduced in 2008, the term "Salivaomics" aimed to highlight the rapid development of knowledge about various "omics" constituents of saliva, including: proteome, transcriptome, micro-RNA, metabolome, and microbiome...
November 29, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27900324/low-expression-of-angiotensinogen-and-dipeptidyl-peptidase-1-in-saliva-of-patients-with-proliferative-verrucous-leukoplakia
#15
Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Adriana Franco Paes Leme, Marcio Ajudarte Lopes
AIM: To elucidate the profile of the salivary proteome. METHODS: Unstimulated whole mouth saliva was collected from 30 volunteers [15 proliferative verrucous leukoplakia (PVL) patients and 15 controls] and proteins were submitted for mass spectrometry-based proteomics using the discovery approach, followed by analyses of variance and logistic regression tests. RESULTS: A total of two hundred and eighty-three proteins were confidently identified in saliva...
November 16, 2016: World Journal of Clinical Cases
https://www.readbyqxmd.com/read/27891302/epidemiology-of-sj%C3%A3-gren-s-syndrome-from-an-oral-perspective
#16
REVIEW
Anne Isine Bolstad, Kathrine Skarstein
Oral symptoms are among the most distressing manifestations for patients with Sjögren's syndrome (SS). The feeling of dry mouth is unpleasant, and hyposalivation may contribute to difficulty in speaking, chewing and swallowing and reduced quality of life. Reduced salivary flow increases the risk for dental caries and problems with prosthetic replacement. It seems that SS is not as frequently occurring as previously anticipated. Population-based prevalence studies on primary SS in Europe, conducted on large background populations and in accordance with the AECG criteria, reported of a prevalence of 1-9 cases per 10,000 people...
2016: Current Oral Health Reports
https://www.readbyqxmd.com/read/27887661/biochemical-and-proteomic-analyses-of-the-physiological-response-induced-by-individual-housing-in-gilts-provide-new-potential-stress-markers
#17
Anna Marco-Ramell, Laura Arroyo, Raquel Peña, Raquel Pato, Yolanda Saco, Lorenzo Fraile, Emøke Bendixen, Anna Bassols
BACKGROUND: The objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort. RESULTS: Blood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals...
November 25, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27802913/proteomic-analysis-of-human-saliva-an-approach-to-find-the-marker-protein-for-ovulation
#18
Ganesan Saibaba, Durairaj Rajesh, Subramanian Muthukumar, Ganesan Sathiyanarayanan, Parasuraman Padmanabhan, Mohammad Abdulkader Akbarsha, Balázs Gulyás, Govindaraju Archunan
Human saliva contains numerous molecules that play a variety of roles. Among them there are proteins which serve as biomarkers of various physiological and/or pathological conditions. Compared to other body fluids, saliva is the most convenient material for investigations, and especially for monitoring the disease conditions. Presently, there is an increasing need to develop a noninvasive method to identify the time of ovulation in humans to ensure successful fertilization, and for evolving strategies for family planning...
December 2016: Reproductive Biology
https://www.readbyqxmd.com/read/27789276/tear-fluid-protein-changes-in-dry-eye-syndrome-associated-with-rheumatoid-arthritis-a-proteomic-approach
#19
Saijyothi Venkata Aluru, Agarwal Shweta, Srinivasan Bhaskar, Krishnan Geetha, Rajappa M Sivakumar, Tatu Utpal, Prema Padmanabhan, Narayanasamy Angayarkanni
PURPOSE: Sjögren syndrome (SS) secondary to rheumatoid arthritis (RA) affects lacrimal and salivary glands, and therefore dry eye syndrome (DES) is more prevalent in patients with RA. This study used a proteomic approach to identify potential biomarkers in tear of DES secondary to RA (DES-RA). METHODS: Tear specimens were collected with Schirmer strips from patients with DES with RA, patients with other types of dry eye (namely, primary Sjögrens and non-Sjögrens [NSS]), and age-matched controls...
January 2017: Ocular Surface
https://www.readbyqxmd.com/read/27716395/thymosin-%C3%AE-4-and-%C3%AE-10-in-sj%C3%A3-gren-s-syndrome-saliva-proteomics-and-minor-salivary-glands-expression
#20
Silvia Bosello, Giusy Peluso, Federica Iavarone, Barbara Tolusso, Irene Messana, Gavino Faa, Massimo Castagnola, Gianfranco Ferraccioli
BACKGROUND: In the present study, we investigated whether thymosin β (Tβ) in saliva and in minor salivary glands is differentially expressed in patients with primary Sjögren's syndrome (pSS) and patients with autoimmune diseases (systemic sclerosis [SSc], systemic lupus erythematosus [SLE], and rheumatoid arthritis [RA], with and without sicca syndrome [ss]). METHODS: Saliva specimens of nine patients with pSS, seven with ss/SSc, seven with ss/SLE, seven with ss/RA, seven with SSc, seven with SLE, and seven with RA, as well as ten healthy subjects, were analyzed using a high-performance liquid chromatograph coupled with a mass spectrometer equipped with an electrospray ionization source to investigate the presence and levels of Tβ4, Tβ4 sulfoxide, and Tβ10...
October 6, 2016: Arthritis Research & Therapy
keyword
keyword
39814
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"