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Salivary proteomics

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https://www.readbyqxmd.com/read/27903834/saliva-diagnostics-current-views-and-directions
#1
Karolina Elżbieta Kaczor-Urbanowicz, Carmen Martin Carreras-Presas, Katri Aro, Michael Tu, Franklin Garcia-Godoy, David Tw Wong
In this review, we provide an update on the current and future applications of saliva for diagnostic purposes. There are many advantages of using saliva as a biofluid. Its collection is fast, easy, inexpensive, and non-invasive. In addition, saliva, as a "mirror of the body," can reflect the physiological and pathological state of the body. Therefore, it serves as a diagnostic and monitoring tool in many fields of science such as medicine, dentistry, and pharmacotherapy. Introduced in 2008, the term "Salivaomics" aimed to highlight the rapid development of knowledge about various "omics" constituents of saliva, including: proteome, transcriptome, micro-RNA, metabolome, and microbiome...
November 29, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27900324/low-expression-of-angiotensinogen-and-dipeptidyl-peptidase-1-in-saliva-of-patients-with-proliferative-verrucous-leukoplakia
#2
Isadora Luana Flores, Alan Roger Santos-Silva, Ricardo Della Coletta, Adriana Franco Paes Leme, Marcio Ajudarte Lopes
AIM: To elucidate the profile of the salivary proteome. METHODS: Unstimulated whole mouth saliva was collected from 30 volunteers [15 proliferative verrucous leukoplakia (PVL) patients and 15 controls] and proteins were submitted for mass spectrometry-based proteomics using the discovery approach, followed by analyses of variance and logistic regression tests. RESULTS: A total of two hundred and eighty-three proteins were confidently identified in saliva...
November 16, 2016: World Journal of Clinical Cases
https://www.readbyqxmd.com/read/27891302/epidemiology-of-sj%C3%A3-gren-s-syndrome-from-an-oral-perspective
#3
REVIEW
Anne Isine Bolstad, Kathrine Skarstein
Oral symptoms are among the most distressing manifestations for patients with Sjögren's syndrome (SS). The feeling of dry mouth is unpleasant, and hyposalivation may contribute to difficulty in speaking, chewing and swallowing and reduced quality of life. Reduced salivary flow increases the risk for dental caries and problems with prosthetic replacement. It seems that SS is not as frequently occurring as previously anticipated. Population-based prevalence studies on primary SS in Europe, conducted on large background populations and in accordance with the AECG criteria, reported of a prevalence of 1-9 cases per 10,000 people...
2016: Current Oral Health Reports
https://www.readbyqxmd.com/read/27887661/biochemical-and-proteomic-analyses-of-the-physiological-response-induced-by-individual-housing-in-gilts-provide-new-potential-stress-markers
#4
Anna Marco-Ramell, Laura Arroyo, Raquel Peña, Raquel Pato, Yolanda Saco, Lorenzo Fraile, Emøke Bendixen, Anna Bassols
BACKGROUND: The objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort. RESULTS: Blood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals...
November 25, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27802913/proteomic-analysis-of-human-saliva-an-approach-to-find-the-marker-protein-for-ovulation
#5
Ganesan Saibaba, Durairaj Rajesh, Subramanian Muthukumar, Ganesan Sathiyanarayanan, Parasuraman Padmanabhan, Mohammad Abdulkader Akbarsha, Balázs Gulyás, Govindaraju Archunan
Human saliva contains numerous molecules that play a variety of roles. Among them there are proteins which serve as biomarkers of various physiological and/or pathological conditions. Compared to other body fluids, saliva is the most convenient material for investigations, and especially for monitoring the disease conditions. Presently, there is an increasing need to develop a noninvasive method to identify the time of ovulation in humans to ensure successful fertilization, and for evolving strategies for family planning...
December 2016: Reproductive Biology
https://www.readbyqxmd.com/read/27789276/tear-fluid-protein-changes-in-dry-eye-syndrome-associated-with-rheumatoid-arthritis-a-proteomic-approach
#6
Saijyothi Venkata Aluru, Agarwal Shweta, Srinivasan Bhaskar, Krishnan Geetha, Rajappa M Sivakumar, Tatu Utpal, Prema Padmanabhan, Narayanasamy Angayarkanni
PURPOSE: Sjögren syndrome (SS) secondary to rheumatoid arthritis (RA) affects lacrimal and salivary glands, and therefore dry eye syndrome (DES) is more prevalent in patients with RA. This study used a proteomic approach to identify potential biomarkers in tear of DES secondary to RA (DES-RA). METHODS: Tear specimens were collected with Schirmer strips from patients with DES with RA, patients with other types of dry eye (namely, primary Sjögrens and non-Sjögrens [NSS]), and age-matched controls...
October 24, 2016: Ocular Surface
https://www.readbyqxmd.com/read/27716395/thymosin-%C3%AE-4-and-%C3%AE-10-in-sj%C3%A3-gren-s-syndrome-saliva-proteomics-and-minor-salivary-glands-expression
#7
Silvia Bosello, Giusy Peluso, Federica Iavarone, Barbara Tolusso, Irene Messana, Gavino Faa, Massimo Castagnola, Gianfranco Ferraccioli
BACKGROUND: In the present study, we investigated whether thymosin β (Tβ) in saliva and in minor salivary glands is differentially expressed in patients with primary Sjögren's syndrome (pSS) and patients with autoimmune diseases (systemic sclerosis [SSc], systemic lupus erythematosus [SLE], and rheumatoid arthritis [RA], with and without sicca syndrome [ss]). METHODS: Saliva specimens of nine patients with pSS, seven with ss/SSc, seven with ss/SLE, seven with ss/RA, seven with SSc, seven with SLE, and seven with RA, as well as ten healthy subjects, were analyzed using a high-performance liquid chromatograph coupled with a mass spectrometer equipped with an electrospray ionization source to investigate the presence and levels of Tβ4, Tβ4 sulfoxide, and Tβ10...
October 6, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27714937/plasmosep-predicting-surface-exposed-proteins-on-the-malaria-parasite-using-semisupervised-self-training-and-expert-annotated-data
#8
Yasser El-Manzalawy, Elyse E Munoz, Scott E Lindner, Vasant Honavar
Accurate and comprehensive identification of surface-exposed proteins (SEPs) in parasites is a key step in developing novel subunit vaccines. However, the reliability of MS-based high-throughput methods for proteome-wide mapping of SEPs continues to be limited due to high rates of false positives (i.e., proteins mistakenly identified as surface exposed) as well as false negatives (i.e., SEPs not detected due to low expression or other technical limitations). We propose a framework called PlasmoSEP for the reliable identification of SEPs using a novel semisupervised learning algorithm that combines SEPs identified by high-throughput experiments and expert annotation of high-throughput data to augment labeled data for training a predictive model...
October 7, 2016: Proteomics
https://www.readbyqxmd.com/read/27703040/the-salivary-protein-repertoire-of-the-polyphagous-spider-mite-tetranychus-urticae-a-quest-for-effectors
#9
Wim Jonckheere, Wannes Dermauw, Vladimir Zhurov, Nicky Wybouw, Jan Van den Bulcke, Carlos A Villarroel, Robert Greenhalgh, Mike Grbić, Rob C Schuurink, Luc Tirry, Geert Baggerman, Richard M Clark, Merijn R Kant, Bartel Vanholme, Gerben Menschaert, Thomas Van Leeuwen
The two-spotted spider mite Tetranychus urticae is an extremely polyphagous crop pest. Alongside an unparalleled detoxification potential for plant secondary metabolites, it has recently been shown that spider mites can attenuate or even suppress plant defenses. Salivary constituents, notably effectors, have been proposed to play an important role in manipulating plant defenses and might determine the outcome of plant-mite interactions. Here, the proteomic composition of saliva from T. urticae lines adapted to various host plants-bean, maize, soy, and tomato-was analyzed using a custom-developed feeding assay coupled with nano-LC tandem mass spectrometry...
December 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27676445/bottom-up-proteomics-suggests-an-association-between-differential-expression-of-mitochondrial-proteins-and-chronic-fatigue-syndrome
#10
F Ciregia, L Kollipara, L Giusti, R P Zahedi, C Giacomelli, M R Mazzoni, G Giannaccini, P Scarpellini, A Urbani, A Sickmann, A Lucacchini, L Bazzichi
Chronic fatigue syndrome (CFS) is a debilitating and complex disorder characterized by unexplained fatigue not improved by rest. An area of investigation is the likely connection of CFS with defective mitochondrial function. In a previous work, we investigated the proteomic salivary profile in a couple of monozygotic twins discordant for CFS. Following this work, we analyzed mitochondrial proteins in the same couple of twins. Nano-liquid chromatography electrospray ionization mass spectrometry (nano-LC-MS) was used to study the mitochondria extracted from platelets of the twins...
September 27, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27672500/metaproteomics-of-saliva-identifies-human-protein-markers-specific-for-individuals-with-periodontitis-and-dental-caries-compared-to-orally-healthy-controls
#11
Daniel Belstrøm, Rosa R Jersie-Christensen, David Lyon, Christian Damgaard, Lars J Jensen, Palle Holmstrup, Jesper V Olsen
BACKGROUND: The composition of the salivary microbiota has been reported to differentiate between patients with periodontitis, dental caries and orally healthy individuals. To identify characteristics of diseased and healthy saliva we thus wanted to compare saliva metaproteomes from patients with periodontitis and dental caries to healthy individuals. METHODS: Stimulated saliva samples were collected from 10 patients with periodontitis, 10 patients with dental caries and 10 orally healthy individuals...
2016: PeerJ
https://www.readbyqxmd.com/read/27669021/salivary-gland-proteome-during-adult-development-and-after-blood-feeding-of-female-anopheles-dissidens-mosquitoes-diptera-culicidae
#12
Benjarat Phattanawiboon, Narissara Jariyapan, Chonlada Mano, Sittiruk Roytrakul, Atchara Paemanee, Sriwatapron Sor-Suwan, Patchara Sriwichai, Atiporn Saeung, Paul A Bates
Understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. Anopheles dissidens (formerly Anopheles barbirostris species A1) is a potential vector of Plasmodium vivax in Thailand. In this study, analyses of the proteomic profiles of female An. dissidens salivary glands during adult development and after blood feeding were carried out using two-dimensional gel electrophoresis coupled with nano-liquid chromatography-mass spectrometry...
2016: PloS One
https://www.readbyqxmd.com/read/27602567/towards-a-proteomic-catalogue-and-differential-annotation-of-salivary-gland-proteins-in-blood-fed-malaria-vector-anopheles-culicifacies-by-mass-spectrometry
#13
Ritu Rawal, Sonam Vijay, Kavita Kadian, Jagbir Singh, Veena Pande, Arun Sharma
In order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. Therefore, in this study as first step, in solution and 1-D electrophoresis digestion approach combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was used to prepare a baseline proteomic catalogue of salivary gland proteins of sugar fed An. culicifacies mosquitoes...
2016: PloS One
https://www.readbyqxmd.com/read/27582331/next-generation-sequencing-of-protein-coding-and-long-non-protein-coding-rnas-in-two-types-of-exosomes-derived-from-human-whole-saliva
#14
Yuko Ogawa, Masafumi Tsujimoto, Ryohei Yanoshita
Exosomes are small extracellular vesicles containing microRNAs and mRNAs that are produced by various types of cells. We previously used ultrafiltration and size-exclusion chromatography to isolate two types of human salivary exosomes (exosomes I, II) that are different in size and proteomes. We showed that salivary exosomes contain large repertoires of small RNAs. However, precise information regarding long RNAs in salivary exosomes has not been fully determined. In this study, we investigated the compositions of protein-coding RNAs (pcRNAs) and long non-protein-coding RNAs (lncRNAs) of exosome I, exosome II and whole saliva (WS) by next-generation sequencing technology...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27561965/the-intracellular-bacterium-anaplasma-phagocytophilum-selectively-manipulates-the-levels-of-vertebrate-host-proteins-in-the-tick-vector-ixodes-scapularis
#15
Margarita Villar, Vladimir López, Nieves Ayllón, Alejandro Cabezas-Cruz, Juan A López, Jesús Vázquez, Pilar Alberdi, José de la Fuente
BACKGROUND: The intracellular bacteria Anaplasma phagocytophilum are emerging zoonotic pathogens affecting human and animal health, and a good model for the study of tick-host-pathogen interactions. This tick-borne pathogen is transmitted by Ixodes scapularis in the United States where it causes human granulocytic anaplasmosis. Tick midguts and salivary glands play a major role during tick feeding and development, and in pathogen acquisition, multiplication and transmission. Vertebrate host proteins are found in tick midguts after feeding and have been described in the salivary glands of fed and unfed ticks, suggesting a role for these proteins during tick feeding and development...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27485005/a-proteomics-approach-reveals-molecular-manipulators-of-distinct-cellular-processes-in-the-salivary-glands-of-glossina-m-morsitans-in-response-to-trypanosoma-b-brucei-infections
#16
Henry M Kariithi, Sjef Boeren, Edwin K Murungi, Just M Vlak, Adly M M Abd-Alla
BACKGROUND: Glossina m. morsitans is the primary vector of the Trypanosoma brucei group, one of the causative agents of African trypanosomoses. The parasites undergo metacyclogenesis, i.e. transformation into the mammalian-infective metacyclic trypomastigote (MT) parasites, in the salivary glands (SGs) of the tsetse vector. Since the MT-parasites are largely uncultivable in vitro, information on the molecular processes that facilitate metacyclogenesis is scanty. METHODS: To bridge this knowledge gap, we employed tandem mass spectrometry to investigate protein expression modulations in parasitized (T...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27478070/a-proteomic-approach-to-compare-saliva-from-individuals-with-and-without-oral-leukoplakia
#17
Danielle Resende Camisasca, Lorena da Rós Gonçalves, Márcia Regina Soares, Vanessa Sandim, Fábio César Sousa Nogueira, Carlos Henrique Saraiva Garcia, Rodrigo Santana, Silvia Paula de Oliveira, Luisa Aguirre Buexm, Paulo Antônio Silvestre de Faria, Fernando Luiz Dias, Denise de Abreu Pereira, Russolina B Zingali, Gilda Alves, Simone Queiroz Chaves Lourenço
BACKGROUND: Oral leukoplakia is the most common potentially malignant disorder in the oral cavity and can precede carcinoma. This study aimed to identify possible oral leukoplakia salivary biomarkers. METHODS: Unstimulated saliva was collected from participants and protein concentration was determined. Proteins were then precipitated with cold acetone and separated using 2DE over a pH range of 3-10. Spot demarcation and matching were performed and protein identification was done through MS analysis...
July 29, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27461598/a-review-on-oral-cancer-biomarkers-understanding-the-past-and-learning-from-the-present
#18
REVIEW
Arvind Babu Rajendra Santosh, Thaon Jones, John Harvey
Biomarkers are broadly classified as genomic, proteomic, or metabolomic. Molecular biology and oncology research studies on oral cancer biomarkers focus on identifying key biological molecules or markers that could be linked to cancer development, risk assessment, screening, recurrence prediction, indicating prognosis, indicating invasion/metastasis and monitoring therapeutic responses of cancer. Cluster of differentiation factor 34 is a salivary biomarker that can identify recurrence potential of oral squamous cell carcinoma (OSCC)...
April 2016: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/27431347/the-proteomics-of-saliva-in-sj%C3%A3-gren-s-syndrome
#19
REVIEW
Stergios Katsiougiannis, David T W Wong
One of the main characteristics of primary Sjögren's syndrome (pSS) is chronic dysfunction and destruction of the salivary and lacrimal glands; their secretory biofluids should reflect the glandular biological status. Saliva is a heterogeneous biofluid comprised of biomolecules and omics constituents that are altered in response to various diseases. Scientific effort has evaluated saliva proteome to diagnose, monitor, and prognosticate pSS. This article reviews the recent advances in salivary proteomics in the context of pSS, highlighting the most significant and promising findings...
August 2016: Rheumatic Diseases Clinics of North America
https://www.readbyqxmd.com/read/27430277/salivary-pellets-induce-a-pro-inflammatory-response-involving-the-tlr4-nf-kb-pathway-in-gingival-fibroblasts
#20
Heinz-Dieter H-D Müller, Barbara B Cvikl, Adrian A Lussi, Reinhard R Gruber
BACKGROUND: Whole saliva provokes a substantial pro-inflammatory response in gingival fibroblasts. This raises the question whether the salivary pellet, which is used for diagnostic purposes, also has a pro-inflammatory capacity and, if yes, what the underlying mechanisms at the molecular level are. METHODS: We examined the ability of extensively washed salivary pellets to provoke the expression of chemokines in gingival fibroblasts by real-time polymerase chain reaction and immunoassays...
2016: BMC Oral Health
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