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Michela Salamone, Teo Martin, Michela Milan, Miquel Costas, Massimo Bietti
A kinetic study on the HAT reactions from the aliphatic C-H bonds of a series of 1-Z-pentyl, 1-Z-propyl and Z-cyclohexyl derivatives, and of a series of N-alkylamides and N-alkylphthalimides to the electrophilic cumyloxyl radical (CumO•) has been carried out. With 1-pentyl and 1-propyl derivatives, α-CH2 activation towards CumO• is observed for Z = Ph, OH, NH2, NHAc, evidenced by an increase in kH as compared to the unsubstituted alkane substrate. A decrease in kH has been instead measured for Z = OAc, NPhth, CO2Me, Cl, Br, CN, indicative of α-CH2 deactivation with HAT that predominantly occurs from the most remote methylenic site...
November 15, 2017: Journal of Organic Chemistry
Naomi Li, Steven Hébert, Jingwen Song, Claudia L Kleinman, Stéphane Richard
The KH-type RNA binding protein Sam68 is required for adipogenesis. We have previously shown that Sam68-deficient mice have a lean phenotype and are protected against dietary-induced obesity due to defects in mTOR and S6K1 alternative splicing. Herein we profiled the transcriptome of Sam68 wild type and deficient 3T3-L1 mouse preadipocytes. We identified 652 protein-coding genes and 9 ncRNAs that were significantly altered with the loss of Sam68. As expected, downregulated genes were significantly associated with GO terms linked to cell migration, motility, and fat cell differentiation, while upregulated genes were mostly associated with GO terms linked to neurogenesis...
October 10, 2017: Oncotarget
I A Miziev, M Kh Makhov
AIM: / To determine the early diagnostic criteria for acute kidney injury in patients with combined trauma using serum cystatin C as a biomarker in the diagnostic work-up of the affected patients. MATERIALS AND METHODS: / The study comprised 42 patients who suffered combined trauma from 2015 to 2016. Cystatin C level was measured in serum. Blood sampling was done on the 1st, 3rd, 7th, 14th day of the injury. The patients were predominantly men (80%). Renal function was tested by measuring the rate of filtration and reabsorption using the Reberg-Tareev test...
October 2017: Urologii︠a︡
Xiang Qian Lao, Zilong Zhang, Alexis Kh Lau, Ta-Chien Chan, Yuan Chieh Chuang, Jimmy Chan, Changqing Lin, Cui Guo, Wun Kai Jiang, Tony Tam, Gerard Hoek, Haidong Kan, Eng-Kiong Yeoh, Ly-Yun Chang
OBJECTIVES: Environmental exposure to chemicals has been considered a potential factor contributing to deteriorated semen quality. However, previous literature on exposure to air pollution and semen quality is inconsistent. We therefore investigated the health effects of short-term and long-term exposure to fine particulate matter (PM2.5) on semen quality in Taiwanese men from the general population. METHODS: A cross-sectional study was conducted among 6475 male participants aged 15-49 years who participated in a standard medical examination programme in Taiwan between 2001 and 2014...
November 13, 2017: Occupational and Environmental Medicine
Valery Kh Akparov, Vladimir I Timofeev, Ilyas G Khaliullin, Vytas Švedas, Inna P Kuranova, Tatiana V Rakitina
No abstract text is available yet for this article.
November 13, 2017: Journal of Biomolecular Structure & Dynamics
Hongmei Zhao, Xiaoshuang Wang, Ping Yi, Yanmin Si, Puwen Tan, Jinrong He, Shan Yu, Yue Ren, Yanni Ma, Junwu Zhang, Dong Wang, Fang Wang, Jia Yu
RNA-binding proteins (RBPs) integrate the processing of RNAs into post-transcriptional gene regulation, but the direct contribution of them to myeloid cell specification is poorly understood. Here, we report the first global RBP transcriptomic analysis of myeloid differentiation by combining RNA-seq analysis with myeloid induction in CD34(+) hematopoietic progenitor cells. The downregulated expression of the KH-Type Splicing Regulatory Protein (KSRP) during monocytopoiesis and up-regulated expression during granulopoiesis suggests that KSRP has divergent roles during monocytic and granulocytic differentiation...
November 10, 2017: Nature Communications
Sarah Kuzmiak-Glancy, Raul Covian, Armel N Femnou, Brian Glancy, Rafael Jaimes, Anastasia M Wengrowski, Kara Garrott, Stephanie French, Robert S Balaban, Matthew W Kay
The left ventricular (LV) working, crystalloid-perfused heart is used extensively to evaluate basic cardiac function, pathophysiology, and pharmacology. Crystalloid-perfused hearts may be limited by oxygen delivery, as adding oxygen carriers increases myoglobin oxygenation and improves myocardial function. However, whether decreased myoglobin oxygen saturation impacts oxidative phosphorylation (OxPhos) is unresolved since myoglobin has a much lower affinity for oxygen than cytochrome c oxidase (COX). In the present study, a laboratory-based synthesis of an affordable perfluorocarbon (PFC) emulsion was developed to increase perfusate oxygen-carrying capacity without impeding optical absorbance assessments...
November 10, 2017: American Journal of Physiology. Heart and Circulatory Physiology
Aigerim Bepeyeva, Joao M S de Barros, Hanady Albadran, Aitbek K Kakimov, Zhaynagul Kh Kakimova, Dimitris Charalampopoulos, Vitaliy V Khutoryanskiy
Gel beads were prepared by extrusion of various types of pectin into 0.15 M calcium chloride. Size, morphology, and textural properties of 3 types of beads were evaluated and it was established that the use of 3 w/v % amidated pectin provides the optimal characteristics suitable for encapsulation of live bacteria. Lactobacillus casei NCIMB 30185 (PXN37) was encapsulated into calcium pectinate gel through the extrusion of a live bacteria dispersion in 3 w/v % pectin into a solution of calcium chloride. The capsules were then additionally coated with chitosan...
November 10, 2017: Journal of Food Science
Loay Kh M Hassouneh, Olga A Timofiychuk, Nataliya A Babenko
Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old age. The 3-, 12- and 24-month-old Wistar male rats were used in the experiments. The progressive increase of ceramide content and ceramide/sphingomyeline (SM) ratio was determined in the extensor digitorum longus (EDL) muscle during aging of rats...
November 10, 2017: General Physiology and Biophysics
S T Mok, M Km Kam, W K Tsang, D Mc Poon, H H Loong, W M Yeung, T Y Yeung, J Yu, C Kh Wong
INTRODUCTION: Stereotactic brain radiosurgery provides good local control in patients with limited brain metastases. A newly developed frameless system allows pain-free treatment. We reviewed the effectiveness of this frameless stereotactic brain radiosurgery and identified prognostic factors that may aid better patient selection. METHODS: Medical records of patients with brain metastases treated with linear accelerator-based frameless stereotactic brain radiosurgery between January 2010 and July 2015 in a university affiliated hospital in Hong Kong were reviewed...
November 10, 2017: Hong Kong Medical Journal, Xianggang Yi Xue za Zhi
V Kh Khavinson, A T Kopylov, B V Vaskovsky, G A Ryzhak, N S Lin'kova
The polypeptide complex of the epiphysis and the peptide AEDG, constructed on the basis of its amino acid analysis, exert similar biological effects. Both bioregulators normalize melatonin synthesis in the pineal gland, functioning of the brain, eye retina, cardiovascular, endocrine, and immune systems; they also act as antioxidants, stress-protectors, and geroprotectors. Within the epiphysis polypeptide complex, free amino acids (3.26%), dipeptides (23.19%), tripeptides (50.72%), tetrapeptides (22.10%), and pentapeptides (0...
November 9, 2017: Bulletin of Experimental Biology and Medicine
Lieke M Spekhorst, Floris Imhann, Eleonora Am Festen, Ad A van Bodegraven, Nanne Kh de Boer, Gerd Bouma, Herma H Fidder, Geert D'Haens, Frank Hoentjen, Daan W Hommes, Dirk J de Jong, Mark Löwenberg, Pw Jeroen Maljaars, Andrea E van der Meulen-de Jong, Bas Oldenburg, Marieke J Pierik, Cyriel Y Ponsioen, Pieter C Stokkers, Hein W Verspaget, Marijn C Visschedijk, C Janneke van der Woude, Gerard Dijkstra, Rinse K Weersma
PURPOSE: The Dutch IBD Biobank aims to facilitate the discovery of predictors for individual disease course and treatment response in patients with inflammatory bowel disease (IBD). In this paper, we aim to describe the establishment of the Dutch IBD Biobank, including the facilitators and barriers to establishment. Moreover, we aim to provide a complete overview of the content of the Dutch IBD Biobank. PARTICIPANTS: Since 2007, every patient with IBD treated in one of the eight Dutch university medical centres is asked to participate in the Dutch IBD Biobank in which 225 standardised IBD-related data items and biomaterials, such as serum, DNA, biopsies and a stool sample, are collected...
November 8, 2017: BMJ Open
Robert-André F Rarig, John M Nelson, Edwin Vedejs
Tetrahydro-1,4-azaborines were accessed by hydroboration of N,N-di-prenyl-toluenesulfonamide 4. Conversion to the methylborinates 11 and 12 followed by heating with l alanine and crystallization afforded (R,R,S)-13 (27%). Hydrolysis to borinic acid (R,R) 18 and reduction with Soderquist's KH* gave the C2-symmetric borohydride (R,R) 19. Subsequent hydride abstraction by TMSCl in the presence of simple alkenes resulted in hydroboration. Promising enantioselectivity was seen with Z alkenes (84-86% ee, RT), but not with E alkenes or 1,1-disubstituted alkenes (<5% ee)...
November 7, 2017: Journal of Organic Chemistry
Miguel A Garcia, Michael A Anderson
Monochloramine is a secondary disinfectant used in drinking water and is also formed in chlorinated wastewater. While known to hydrolyze over time and react with dissolved organic matter, its partitioning between the aqueous and gas phase has not been extensively studied. Preliminary experiments demonstrated that monochloramine concentrations in solutions open to the atmosphere or actively aerated decreased more rapidly than in sealed solutions, indicating significant losses to the atmosphere. For example, a monochloramine solution open to the atmosphere yielded a loss rate constant of 0...
October 30, 2017: Chemosphere
Parisa Tabeshmehr, Haider Kh Husnain, Mahin Salmannejad, Mahsa Sani, Seyed Mojtaba Hosseini, Mohammad Hossein Khorraminejad Shirazi
Background: Extensive loss of donor neural stem cell (NSCs) due to ischemic stress and low rate of differentiation at the site of cell graft are two of the major issues that hamper optimal outcome in NSCs transplantation studies. Given that histone deacetylases (HDACs) modulate various cellular processes by deacetylating histones and non-histone proteins, we hypothesized that combined treatment with small molecules, sodium butyrate (NaB; a known HDAC inhibitor) and nicorandil, will enhance the rate neuronal differentiation of NSCs besides their preconditioning to resist oxidative stress...
2017: Translational Neurodegeneration
Giulia Guerriero, Maria Violetta Brundo, Sofiane Labar, Anna Rita Bianchi, Samantha Trocchia, Dea Rabbito, Giancarlo Palumbo, Fagr Kh Abdel-Gawad, Anna De Maio
Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm species not considered threatened by the IUCN, sampled in representative sites at different degree. In the first phase, a biochemical characterization of the ADP-ribosylation on the skin of barcoded amphibian anura collected from Matese Lake (clean reference site in CE, Italy) was carried out. Two PARP isoforms were evidence: the first of 66 kDa is localized into nucleus and activated by DNA damage; the second of 150 kDa is in cytoplasm, as demonstrated by biochemical and immunohistochemical analysis...
October 28, 2017: Environmental Science and Pollution Research International
A A Sazanov, Kh Kh Erganokov, E Pfeifer
Biobanks are systematic and annotated collections of biological samples based on the system of standard operating procedures (SOP) and corresponding to the recommendations of the International Society for Biological and Environmental Repositories (ISBER). Standardization of conditions of obtaining, processing, storage of samples and providing to an end user are crucial in the activities of the biobank. The attributes of biobanks include common principles of labeling and annotation of biological samples using specialized software, an automated monitoring system of storage conditions, and registration of biosamples...
October 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
Hyun Jun Park, Yean Kyoung Koo, Min Jung Park, Yoon Kyung Hwang, Sung Yeoun Hwang, Nam Cheol Park
PURPOSE: We investigated the protective effect of a mixture of 2 herbal extracts, KH-465, which consisted of Epimedium koreanum Nakai and Angelica gigas Nakai, on spermatogenesis in a luteinizing hormone-releasing hormone (LHRH) agonist-induced rat model of male infertility. MATERIALS AND METHODS: Seventy-five 12-week-old male Sprague-Dawley rats were randomly divided into 5 groups, containing 15 rats each: a normal control group that received no treatment and 4 experimental groups (I, II, III, and IV) in which an LHRH agonist was administered for 4 weeks to induce spermatogenic failure...
October 25, 2017: World Journal of Men's Health
Zongyun Yan, Jianheng Jia, Xiaoyuan Yan, Huiying Shi, Yuzhen Han
The two novel CCCH zinc-finger and K-homolog (KH) proteins, KHZ1 and KHZ2, play important roles in regulating flowering and senescence redundantly in Arabidopsis. The CCCH zinc-finger proteins and K-homolog (KH) proteins play important roles in plant development and stress responses. However, the biological functions of many CCCH zinc-finger proteins and KH proteins remain uncharacterized. In Arabidopsis, KHZ1 and KHZ2 are characterized as two novel CCCH zinc-finger and KH domain proteins which belong to subfamily VII in CCCH family...
October 26, 2017: Plant Molecular Biology
Woong Jin Bae, Guan Qun Zhu, Sae Woong Choi, Hyun Cheol Jeong, Fahad Bashraheel, Kang Sup Kim, Su Jin Kim, Hyuk Jin Cho, U Syn Ha, Sung Hoo Hong, Ji Youl Lee, Hyun-A Oh, Hye Cheong Koo, Do Ram Kim, Sung Yeoun Hwang, Sae Woong Kim
The Korean herbal formulation Ojayeonjonghwan is used for improving late-onset hypogonadism (LOH) symptoms such as erectile dysfunction (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to the treatment for ED. The pharmacological effects were examined in different conditions, including in vitro and in vivo. We measured the survival rate of TM3 Leydig cells under the oxidative stress condition. The s.c. injection of leuprorelin was used to induce androgen deprivation...
2017: Oxidative Medicine and Cellular Longevity
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