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https://www.readbyqxmd.com/read/28072657/assessment-of-cochlea-endolymphatic-hydrops-using-3-d-flair-and-3-d-real-ir-sequence-in-guinea-pigs-via-3t-mri-after-intratympanic-gadolinium-a-histopathological-comparison
#1
Feng Wang, Huiying Lyu, Menglong Zhao, Yan Sha, Fang Zhang, Yushu Cheng, Wenhu Huang, Wenlin Tang, Youzhou Xie, Ping Lu
OBJECTIVE: We assessed whether the three-dimensional fluid-attenuated inversion-recovery (3-D FLAIR) and three-dimensional inversion-recovery with real reconstruction (3-D real IR) sequences can be used to detect cochlea endolymphatic hydrops (EHs) in guinea pigs using 3 Tesla magnetic resonance imaging (3T MRI). The results of 3-D real IR imaging were compared with histopathological outcomes. MATERIALS AND METHODS: Fourteen healthy men and women albino guinea pigs were used in this study...
January 9, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/27997841/rna-transport-from-head-to-toe-in-radial-glial-cells
#2
Janina Ehses, Michael A Kiebler, Sandra M Fernández-Moya
Intracellular mRNA localization critically contributes to proper brain development and function. A recent study demonstrates that mRNAs are actively transported in radial glial cells from the soma to the distal basal endfeet, where they are locally translated.
December 19, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27939951/effects-of-personalised-exposure-on-self-rated-electromagnetic-hypersensitivity-and-sensibility-a-double-blind-randomised-controlled-trial
#3
Imke van Moorselaar, Pauline Slottje, Pia Heller, Rob van Strien, Hans Kromhout, Manuel Murbach, Niels Kuster, Roel Vermeulen, Anke Huss
BACKGROUND: Previous provocation experiments with persons reporting electromagnetic hypersensitivity (EHS) have been criticised because EHS persons were obliged to travel to study locations (seen as stressful), and that they were unable to select the type of signal they reported reacting to. In our study we used mobile exposure units that allow double-blind exposure conditions with personalised exposure settings (signal type, strength, duration) at home. Our aim was to evaluate whether subjects were able to identify exposure conditions, and to assess if providing feedback on personal test results altered the level of self-reported EHS...
December 9, 2016: Environment International
https://www.readbyqxmd.com/read/27939136/electromagnetic-hypersensitivity-ehs-in-occupational-and-primary-health-care-a-nation-wide-survey-among-general-practitioners-occupational-physicians-and-hygienists-in-the-netherlands
#4
Pauline Slottje, Imke van Moorselaar, Rob van Strien, Roel Vermeulen, Hans Kromhout, Anke Huss
Subjects who attribute health complaints to every day levels of non-ionizing electromagnetic fields (EMF) have been referred to as electrohypersensitive (EHS). Previous surveys in Europe showed that 68-75% of general practitioners had ever been consulted on EHS. Given the lack of data on EHS in the Netherlands in the general population and on EHS in occupational settings, we performed a national survey among three professional groups that are likely in the first line of being consulted by EHS individuals. Results show that about one third of occupational hygienists, occupational physicians and general practitioners had ever been consulted by one or more EHS subjects...
December 2, 2016: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/27938990/effect-of-low-energy-linear-shockwave-therapy-on-erectile-dysfunction-a-double-blinded-sham-controlled-randomized-clinical%C3%A2-trial
#5
Grzegorz L Fojecki, Stefan Tiessen, Palle J S Osther
INTRODUCTION: Previous studies have shown that focal low-energy extracorporeal shockwave therapy (Li-ESWT) can have a positive effect in men with erectile dysfunction (ED). Linear Li-ESWT (LLi-ESWT) for ED has not been previously assessed in a randomized trial. AIM: To evaluate the treatment outcome of LLi-ESWT for ED. METHODS: Men with ED (n = 126) and a score lower than 25 points on the International Index of Erectile Function erectile function domain (IIEF-EF) were included...
December 6, 2016: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/27935464/effects-of-hypnosis-on-regression-to-primary-process-thinking
#6
Gary Grogan, Arreed Barabasz, Marianne Barabasz, Ciara Christensen
The authors tested the hypothesis that hypnotic age regression produces a shift from secondary- to primary-thought processing in hypnotizable participants. Thirty participants were tested for hypnotizability using the Elkins Scale of Hypnotizability (EHS). Participants were exposed to a tailored hypnotic arm-levitation induction and a hypnotic suggestion to age regress to 5 years of age and to have a dream. Primary-process thinking was assessed using the Primary-Process Thinking Scale. An independent t test with 2 levels of hypnotizability (middle and high) showed high hypnotizables demonstrated higher ratings of primary thought processing than those in the middle group...
January 2017: International Journal of Clinical and Experimental Hypnosis
https://www.readbyqxmd.com/read/27933397/laparo-endoscopic-versus-open-recurrent-inguinal-hernia-repair-should-we-follow-the-guidelines
#7
F Köckerling, R Bittner, A Kuthe, B Stechemesser, R Lorenz, A Koch, W Reinpold, H Niebuhr, M Hukauf, C Schug-Pass
INTRODUCTION: On the basis of six meta-analyses, the guidelines of the European Hernia Society (EHS) recommend laparo-endoscopic recurrent repair following previous open inguinal hernia operation and, likewise, open repair following previous laparo-endoscopic operation. So far no data are available on implementation of the guidelines or for comparison of outcomes. Besides, there are no studies for comparison of outcomes for compliance versus non-compliance with the guidelines. PATIENTS AND METHODS: In total, 4812 patients with elective unilateral recurrent inguinal hernia repair in men were enrolled between September 1, 2009, and September 17, 2014, in the Herniamed Registry...
December 8, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27909226/unique-cytokine-and-chemokine-responses-to-exertional-heat-stroke-in-mice
#8
Michelle A King, Lisa R Leon, Deborah A Morse, Thomas L Clanton
In heat stroke, cytokines are believed to play important roles in multi-organ dysfunction and recovery of damaged tissue. The time course of the cytokine response is well defined in passive heat stroke (PHS), but little is known about exertional heat stroke (EHS). In this study we used a recently developed mouse EHS model to measure the responses of circulating cytokines/chemokines and cytokine gene expression in muscle. A very rapid increase in circulating IL-6 was observed at max core temperature (Tc,max) that peaked at 0...
December 1, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27900794/fast-and-efficient-free-induction-decay-mr-spectroscopic-imaging-of-the-human-brain-at-9-4-tesla
#9
Grzegorz L Chadzynski, Jonas Bause, Gunamony Shajan, Rolf Pohmann, Klaus Scheffler, Philipp Ehses
PURPOSE: The purpose of this work was to develop a fast and efficient MRSI-FID acquisition scheme and test its performance in vivo. The aim was to find a trade-off between the minimal total acquisition time and signal-to-noise ratio of the acquired spectra. METHODS: Measurements were performed on a 9.4 Tesla system. Sequence optimization included redesign of water suppression, optimization of the sequence gradients, and improvement of the sampling efficiency by minimizing the read-out time...
November 29, 2016: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/27796954/using-3d-culture-of-primary-mammary-epithelial-cells-to-define-molecular-entities-required-for-acinus-formation-analyzing-map-kinase-phosphatases
#10
Malgorzata Gajewska, Sara McNally
Three-dimensional (3D) cell cultures on reconstituted basement membrane (rBM) enable the study of complex interactions between extracellular matrix (ECM) components and epithelial cells, which are crucial for the establishment of cell polarity and functional development of epithelia. 3D cultures of mammary epithelial cells (MECs) on Matrigel (a laminin-rich ECM derived from the Engelbreth-Holm-Swarm (EHS) murine tumor) promote interactions of MECs with the matrix via integrins, leading to formation of spherical monolayers of polarized cells surrounding a hollow lumen (acini)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27795395/exported-epoxide-hydrolases-modulate-erythrocyte-vasoactive-lipids-during-plasmodium-falciparum-infection
#11
Natalie J Spillman, Varun K Dalmia, Daniel E Goldberg
: Erythrocytes are reservoirs of important epoxide-containing lipid signaling molecules, including epoxyeicosatrienoic acids (EETs). EETs function as vasodilators and anti-inflammatory modulators in the bloodstream. Bioactive EETs are hydrolyzed to less active diols (dihydroxyeicosatrienoic acids) by epoxide hydrolases (EHs). The malaria parasite Plasmodium falciparum infects host red blood cells (RBCs) and exports hundreds of proteins into the RBC compartment. In this study, we show that two parasite epoxide hydrolases, P falciparum epoxide hydrolases 1 (PfEH1) and 2 (PfEH2), both with noncanonical serine nucleophiles, are exported to the periphery of infected RBCs...
October 18, 2016: MBio
https://www.readbyqxmd.com/read/27789366/controlled-drug-release-from-hydrogels-for-contact-lenses-drug-partitioning-and-diffusion
#12
A F R Pimenta, J Ascenso, J C S Fernandes, R Colaço, A P Serro, B Saramago
Optimization of drug delivery from drug loaded contact lenses assumes understanding the drug transport mechanisms through hydrogels which relies on the knowledge of drug partition and diffusion coefficients. We chose, as model systems, two materials used in contact lens, a poly-hydroxyethylmethacrylate (pHEMA) based hydrogel and a silicone based hydrogel, and three drugs with different sizes and charges: chlorhexidine, levofloxacin and diclofenac. Equilibrium partition coefficients were determined at different ionic strength and pH, using water (pH 5...
December 30, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27738568/liver-transplantation-after-exertional-heatstroke-induced-acute-liver-failure
#13
Faisal Inayat, Hafeez Ul Hasan Virk
Exertional heatstroke (EHS) is a life-threatening disease characterized clinically by central nervous system dysfunction and severe hyperthermia. It frequently occurs among athletes, soldiers, and laborers. While cardiopulmonary symptoms are common in patients undergoing EHS, irreversible acute liver failure is a rarely described phenomenon. When managing cases of EHS complicated by acute liver failure, it is crucial to act promptly with aggressive total body cooling in order to prevent progression of the clinical syndrome...
September 6, 2016: Curēus
https://www.readbyqxmd.com/read/27715394/orphan-g-protein-coupled-receptor-gprc5a-modulates-integrin-%C3%AE-1-mediated-epithelial-cell-adhesion
#14
Daria R Bulanova, Yevhen A Akimov, Anne Rokka, Teemu D Laajala, Tero Aittokallio, Petri Kouvonen, Teijo Pellinen, Sergey G Kuznetsov
G-Protein Coupled Receptor (GPCR), Class C, Group 5, Member A (GPRC5A) has been implicated in several malignancies. The underlying mechanisms, however, remain poorly understood. Using a panel of human cell lines, we demonstrate that CRISPR/Cas9-mediated knockout and RNAi-mediated depletion of GPRC5A impairs cell adhesion to integrin substrates: collagens I and IV, fibronectin, as well as to extracellular matrix proteins derived from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma (Matrigel). Consistent with the phenotype, knock-out of GPRC5A correlated with a reduced integrin β1 (ITGB1) protein expression, impaired phosphorylation of the focal adhesion kinase (FAK), and lower activity of small GTPases RhoA and Rac1...
October 7, 2016: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/27705730/erectile-function-recovery-after-robotic-assisted-radical-prostatectomy-rarp-long-term-exhaustive-analysis-across-all-preoperative-potency-categories
#15
Abdullah M Alenizi, Kevin C Zorn, Marc Bienz, Emad Rajih, Pierre Alain Hueber, Naif Al-Hathal, Serge Benayoun, Thierry Lebeau, Assaad El-Hakim
INTRODUCTION: To evaluate erectile function recovery following robotic-assisted radical prostatectomy (RARP) according to preoperative sexual health inventory for men (SHIM) score stratification. MATERIALS AND METHODS: We prospectively collected data on 250 consecutive patients who underwent RARP by a single surgeon between October 2006 and October 2012. Thirty-six patients were excluded because of lack of preoperative SHIM score. All patients had a minimum follow up of 2 years...
October 2016: Canadian Journal of Urology
https://www.readbyqxmd.com/read/27687595/differentiation-of-five-enterohepatic-helicobacter-species-by-nested-pcr-with-high-resolution-melting-curve-analysis
#16
Miaoli Wu, Dan Rao, Yujun Zhu, Jing Wang, Wen Yuan, Yu Zhang, Ren Huang, Pengju Guo
BACKGROUND: Enterohepatic Helicobacter species (EHS) are widespread in rodent species around the world. Several studies have demonstrated that infection with EHS can interfere with the outcomes of animal experiments in cancer research and significantly influence the study results. Therefore, it is essential to establish a rapid detection and identification of EHS for biomedical research using laboratory rodents. Our study aimed to develop a rapid and sensitive method to detect and distinguish five enterohepatic Helicobacter species...
September 30, 2016: Helicobacter
https://www.readbyqxmd.com/read/27663989/whole-brain-mp2rage-based-mapping-of-the-longitudinal-relaxation-time-at-9-4t
#17
G E Hagberg, J Bause, T Ethofer, P Ehses, T Dresler, C Herbert, R Pohmann, G Shajan, A Fallgatter, M A Pavlova, K Scheffler
Mapping of the longitudinal relaxation time (T1) with high accuracy and precision is central for neuroscientific and clinical research, since it opens up the possibility to obtain accurate brain tissue segmentation and gain myelin-related information. An ideal, quantitative method should enable whole brain coverage within a limited scan time yet allow for detailed sampling with sub-millimeter voxel sizes. The use of ultra-high magnetic fields is well suited for this purpose, however the inhomogeneous transmit field potentially hampers its use...
January 1, 2017: NeuroImage
https://www.readbyqxmd.com/read/27659062/depth-dependence-of-visual-signals-in-the-human-superior-colliculus-at-9-4-t
#18
Joana R Loureiro, Gisela E Hagberg, Thomas Ethofer, Michael Erb, Jonas Bause, Philipp Ehses, Klaus Scheffler, Marc Himmelbach
The superior colliculus (SC) is a layered structure located in the midbrain. We exploited the improved spatial resolution and BOLD signal strength available at 9.4 T to investigate the depth profile of visual BOLD responses in the human SC based on distortion-corrected EPI data with a 1 mm isotropic resolution. We used high resolution (350 µm in-plane) anatomical images to determine regions-of-interest of the SC and applied a semi-automated method to segment it into superficial, intermediate, and deep zones...
January 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/27632869/can-temperate-water-immersion-effectively-reduce-rectal-temperature-in-exertional-heat-stroke-situations-a-critically-appraised-topic
#19
Tyler T Truxton, Kevin C Miller
Clinical Scenario Exertional heat stroke (EHS) is a medical emergency which, if left untreated, can result in death. The standard of care for EHS patients includes confirmation of hyperthermia via rectal temperature (Trec) and then immediate cold-water immersion (CWI). While CWI is the fastest way to reduce Trec,(1,2) it may be difficult to lower and maintain water bath temperature in the recommended ranges (1.7°C-15°C [35°F-59°F])(1) because of limited access to ice and/or the bath being exposed to high ambient temperatures for long periods of time...
August 24, 2016: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/27632849/should-cooling-vests-be-used-to-treat-exertional-heatstroke-a-critically-appraised-topic
#20
Megan L Keen, Kevin C Miller
: Clinical Scenario: Exercise performed in hot and humid environments increases core body temperature (TC). If TC exceeds 40°C for prolonged periods of time, exertional heat stroke (EHS) may occur. EHS is one of the leading causes of sudden death in athletes. Mortality and morbidity increase the longer the patient's TC remains above 40°C; thus, it is imperative to initiate cooling as quickly as possible. "Acceptable" cooling rates in EHS situations are between 0.08°C·min-1 and 0.15°C·min-1 while "ideal" cooling rates are above 0...
August 24, 2016: Journal of Sport Rehabilitation
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