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https://www.readbyqxmd.com/read/29130959/ginsenoside-rg1-protects-cardiomyocytes-against-hypoxia-reoxygenation-injury-via-activation-of-nrf2-ho-1-signaling-and-inhibition-of-jnk
#1
Qianhui Li, Yin Xiang, Yu Chen, Yong Tang, Yachen Zhang
BACKGROUND/AIMS: Excessive reactive oxygen species (ROS) disturb the physiology of H9c2 cells, which is regarded as a major cause of H9c2 cardiomyocyte apoptosis. Ginsenoside Rg1 is the main active extract of ginseng, which has important antioxidant properties in various cell models. This project investigated the role of ginsenoside Rg1 in hypoxia/reoxygenation (H/R)-induced oxidative stress injury in cultured H9c2 cells to reveal the underlying signaling pathways. METHODS: H9c2 cells were pretreated with ginsenoside Rg1 for 12 h before exposure to H/R...
November 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29019920/pratol-an-o-methylated-flavone-induces-melanogenesis-in-b16f10-melanoma-cells-via-p-p38-and-p-jnk-upregulation
#2
You Chul Chung, Seoyeon Kim, Jin Hwa Kim, Geun Soo Lee, Jung No Lee, Nam Ho Lee, Chang-Gu Hyun
Tyrosinase is the rate-limiting enzyme critical for melanin synthesis. It controls pigmentation in the skin. Activation of tyrosinase is currently the most common approach in the development of tanning and haircare products. Pratol is a 7-hydroxy-4-methoxyflavone found in Trifoliumpratense. In this study, we investigated the effects of pratol on melanogenesis. We also studied the mechanism of action of pratol in B16F10 mouse melanoma cells. The cells were treated with various concentrations (6.25, 12.5, 25, and 50 μM) of pratol to observe its effects...
October 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29018002/glucose-uptake-inhibition-decreases-expressions-of-receptor-activator-of-nuclear-factor-kappa-b-ligand-rankl-and-osteocalcin-in-osteocytic-mlo-y4-a2-cells
#3
Ayumu Takeno, Ippei Kanazawa, Masakazu Notsu, Ken-Ichiro Tanaka, Toshitsugu Sugimoto
Bone and glucose metabolism are closely associated with each other. Both osteoblast and osteoclast functions are important for the action of osteocalcin, which plays pivotal roles as an endocrine hormone regulating glucose metabolism. However, it is unknown whether osteocytes are involved in the interaction between bone and glucose metabolism. We used MLO-Y4-A2, a murine long bone-derived osteocytic cell line, to investigate effects of glucose uptake inhibition on expressions of osteocalcin and bone-remodeling modulators in osteocytes...
October 10, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28968596/resveratrol-inhibits-the-epidermal-growth-factor-induced-migration-of-osteoblasts-the-suppression-of-sapk-jnk-and-akt
#4
Tetsu Kawabata, Haruhiko Tokuda, Kazuhiko Fujita, Shingo Kainuma, Go Sakai, Rie Matsushima-Nishiwaki, Osamu Kozawa, Takanobu Otsuka
BACKGROUND/AIMS: Resveratrol is a polyphenol enriched in the skins of grapes and berries, that shows various beneficial effects for human health. In the present study, we investigated the mechanism behind the epidermal growth factor (EGF)-induced migration of osteoblast-like MC3T3-E1 cells, and the effect of resveratrol on this cell migration. METHODS: The cell migration was examined using Boyden chamber, and phosphorylation of each kinase was analyzed by Western blotting...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28951356/-expressions-of-inflammatory-cytokines-in-mouse-peritoneal-macrophages-induced-by-mrp8-mrp14-in-vitro
#5
Juan Wang, Lei Li, Hai-Hua Luo, Yong Jiang
OBJECTIVE: To investigate MRP8/MRP14-induced expression of inflammatory cytokines in mouse peritoneal macrophages and explore the mechanism. METHODS: Tumor necrosis factor-α (TNF-α), interferon-γ inducible protein 10 (IP-10), interleukin-2 (IL-2), IL-6, IL-5 and interferon-γ (IFN-γ) proteins in the culture supernatants of mouse peritoneal macrophages treated with recombinant MRP8/MRP14 were quantified using Luminex xMAP system. TNF-α, IP-10 and IL-6 levels were detected in the culture supernatants of the peritoneal macrophages after treatment with different domains of MRP14...
September 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28902366/heat-shock-protein-27-hspb1-suppresses-the-pdgf-bb-induced-migration-of-osteoblasts
#6
Shingo Kainuma, Haruhiko Tokuda, Naohiro Yamamoto, Gen Kuroyanagi, Kazuhiko Fujita, Tetsu Kawabata, Go Sakai, Rie Matsushima-Nishiwaki, Osamu Kozawa, Takanobu Otsuka
Heat shock protein 27 (HSP27/HSPB1), one of the small heat shock proteins, is constitutively expressed in various tissues. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor‑BB (PDGF‑BB)‑stimulated migration of osteoblast-like MC3T3-E1 cells. PDGF-BB by itself barely upregulated the expression of HSP27 protein, but stimulated the phosphorylation of HSP27 in these cells...
October 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28878808/luteolin-quercetin-genistein-and-quercetagetin-inhibit-the-effects-of-lipopolysaccharide-obtained-from-porphyromonas-gingivalis-in-h9c2-cardiomyoblasts
#7
Gloria Gutiérrez-Venegas, Alfredo Torras-Ceballos, Juan Arturo Gómez-Mora, Berenice Fernández-Rojas
BACKGROUND: One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from P. gingivalis promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2)...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28842382/varenicline-promotes-endothelial-cell-migration-by-lowering-vascular-endothelial-cadherin-levels-via-the-activated-%C3%AE-7-nicotinic-acetylcholine-receptor-mitogen-activated-protein-kinase-axis
#8
Mitsuhisa Koga, Yuki Kanaoka, Keita Sugiyama, Kaoru Ohishi, Yuka Ejima, Mami Hisanaga, Yasufumi Kataoka, Atsushi Yamauchi
Varenicline is a widely used and effective drug for smoking cessation. Despite its efficacy, varenicline increases the risk of cardiovascular disease. We previously demonstrated that varenicline aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice. However, little is known about its effects in vascular endothelial cells. Therefore, we examined whether varenicline promotes migration of human umbilical vein endothelial cells (HUVECs) using the Boyden chamber assay. Varenicline (100μM) markedly promoted migration of HUVECs and decreased expression of vascular endothelial (VE)-cadherin, an endothelial adhesion molecule...
September 1, 2017: Toxicology
https://www.readbyqxmd.com/read/28656249/oxidative-stress-regulates-mitogen%C3%A2-activated-protein-kinases-and-c%C3%A2-jun-activation-involved-in-heat-stress-and-lipopolysaccharide%C3%A2-induced-intestinal-epithelial-cell-apoptosis
#9
Yanan Liu, Zhenglian Wang, Weidang Xie, Zhengtao Gu, Qiulin Xu, Lei Su
Heat stress and gut‑derived endotoxinemia are common causes of multiple organ dysfunction syndrome in heat stroke patients. Evidence has demonstrated that cell apoptosis in the small intestine serves an important role in the pathogenesis of heatstroke, which leads to increased intestinal permeability to endotoxin or lipopolysaccharides (LPS) from the gut entering the circulation. However, little is known about the potential underlying mechanisms mediating heat stress combined with LPS‑induced intestinal epithelial cell apoptosis...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627696/desipramine-induces-apoptosis-in-hepatocellular-carcinoma-cells
#10
Dong Kwon Yang, Shang-Jin Kim
Antitumor effects of antidepressants have been reported in many cancer cell lines. However, anti-proliferative effects of desipramine, a tricyclic antidepressant, in hepatocellular carcinoma are currently unknown. In this study, we examined the effects of desipramine in human hepatoma Hep3B cells. To evaluate anti-proliferative effects of desipramine in Hep3B cells, we determined cell viability, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), mitogen-activated protein kinase (MAPK) activity, and intracellular Ca2+ levels after desipramine treatment...
June 15, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28601021/aspergillus-fumigatus-induced-early-inflammatory-response-in-pulmonary-microvascular-endothelial-cells-role-of-p38-mapk-and-inhibition-by-silibinin
#11
Jun Song, Weihua Pan, Yue Sun, Jing Han, Weimin Shi, Wanqing Liao
Human invasive pulmonary aspergillosis (IPA) is a serious infectious disease mainly caused by Aspergillus fumigatus (A. fumigatus). Pulmonary microvascular endothelial cells (PMVECs) are important ones in the human lung tissue. However, it remains unclear about the role of PMVECs in IPA. In the present study, we cocultured PMVECs with A. fumigatus. We observed that A. fumigatus induced dose- and time-dependent increases of interleukin 6 (IL-6), interleukin 1β (IL-1β) and intercellular adhesion molecule 1 (ICAM-1) concentration in the cultures...
June 7, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28598073/-effects-of-fgf-21-on-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-in-high-glucose-environment
#12
Xi Li, Cheng Chen, Zhen-Mei An, Yu-Jue Li, Mei Zhang, He He, Shuang-Qing Li
OBJECTIVES: To determine the effect of fibroblast growth factor-21(FGF-21)on the osteogenic differention of human bone mesenchymal stem cells (hBMSCs) exposed to a hyperglycemia condition in vitro. METHODS: hBMSCs were isolated from adult bone marrows, and identified by Alizarin red and oil red O staining. The expressions of immunophenotype were analysed using flow cytometry (CD105, CD90, CD73, CD44).HBMSCs were divided into control group[glucose (Glu) concentration of 5...
September 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28494003/p2y6-receptors-are-involved-in-mediating-the-effect-of-inactivated-avian-influenza-virus-h5n1-on-il-6-cxcl8-mrna-expression-in-respiratory-epithelium
#13
Nawiya Huipao, Suparerk Borwornpinyo, Suwimon Wiboon-Ut, Craig R Campbell, Il-Ha Lee, Siriphun Hiranyachattada, Chonlaphat Sukasem, Arunee Thitithanyanont, Chumpol Pholpramool, David I Cook, Anuwat Dinudom
One of the key pathophysiologies of H5N1 infection is excessive proinflammatory cytokine response (cytokine storm) characterized by increases in IFN-β, TNF-α, IL-6, CXCL10, CCL4, CCL2 and CCL5 in the respiratory tract. H5N1-induced cytokine release can occur via an infection-independent mechanism, however, detail of the cellular signaling involved is poorly understood. To elucidate this mechanism, the effect of inactivated (β-propiolactone-treated) H5N1 on the cytokine and chemokine mRNA expression in 16HBE14o- human respiratory epithelial cells was investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28437005/convergent-erk1-2-p38-and-jnk-mitogen-activated-protein-kinases-mapks-signalling-mediate-catecholoestradiol-induced-proliferation-of-ovine-uterine-artery-endothelial-cells
#14
Rosalina Villalon Landeros, Sheikh O Jobe, Gabrielle Aranda-Pino, Gladys E Lopez, Jing Zheng, Ronald R Magness
KEY POINTS: The catechol metabolites of 17β-oestradiol (E2 β), 2-hydroxyoestradiol (2-OHE2 ) and 4-hydroxyoestradiol (4-OHE2 ), stimulate proliferation of pregnancy-derived ovine uterine artery endothelial cells (P-UAECs) through β-adrenoceptors (β-ARs) and independently of the classic oestrogen receptors (ERs). Herein we show that activation of ERK1/2, p38 and JNK mitogen activated protein kinases (MAPKs) is necessary for 2-OHE2 - and 4-OHE2 -induced P-UAEC proliferation, as well as proliferation induced by the parent hormone E2 β and other β-AR signalling hormones (i...
July 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28316375/torilin-inhibits-inflammation-by-limiting-tak1-mediated-map-kinase-and-nf-%C3%AE%C2%BAb-activation
#15
Mehari Endale, Tae-Hwan Kim, Yi-Seong Kwak, Na-Mi Kim, Seung-Hyung Kim, Jae Youl Cho, Bong-Sik Yun, Man-Hee Rhee
Torilin, a sesquiterpene isolated from the fruits of Torilis japonica, has shown antimicrobial, anticancer, and anti-inflammatory properties. However, data on the mechanism of torilin action against inflammation is limited. This study aimed at determining the anti-inflammatory property of torilin in LPS-induced inflammation using in vitro model of inflammation. We examined torilin's effect on expression levels of inflammatory mediators and cytokines in LPS-stimulated RAW 264.7 macrophages. The involvement of NF-kB and AP-1, MAP kinases, and adaptor proteins were assessed...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28123510/effects-of-prunus-mume-siebold-zucc-in-the-pacemaking-activity-of-interstitial-cells-of-cajal-in-murine-small-intestine
#16
Sang Weon Lee, Sung Jin Kim, Hyungwoo Kim, Dongki Yang, Hyun Jung Kim, Byung Joo Kim
Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28115173/cyclosporine-counteracts-endotoxemia-evoked-reductions-in-blood-pressure-and-cardiac-autonomic-dysfunction-via-central-sgc-mapks-signaling-in-rats
#17
Marwa Y Sallam, Sahar M El-Gowilly, Abdel-Galil A Abdel-Galil, Mahmoud M El-Mas
The immunosuppressant drug cyclosporine A (CSA) improves survivability in endotoxemia and offsets associated loss in vascular reactivity and hypotension. We tested the hypothesis that central phosphoinositide-3-kinase (PI3K)/soluble guanylate cyclase (sGC)/mitogen activated protein kinases (MAPKs) cascade modulates the CSA counteraction of endotoxic hypotension and cardiac autonomic dysfunction. The effects of pharmacologic inhibition of these molecular substrates in central pools on CSA interaction with cardiovascular responses evoked by lipopolysaccharide (LPS) were evaluated in conscious rats...
February 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28112176/bx-795-inhibits-hsv-1-and-hsv-2-replication-by-blocking-the-jnk-p38-pathways-without-interfering-with-pdk1-activity-in-host-cells
#18
Ai-Rong Su, Min Qiu, Yan-Lei Li, Wen-Tao Xu, Si-Wei Song, Xiao-Hui Wang, Hong-Yong Song, Nan Zheng, Zhi-Wei Wu
BX-795 is an inhibitor of 3-phosphoinositide-dependent kinase 1 (PDK1), but also a potent inhibitor of the IKK-related kinase, TANKbinding kinase 1 (TBK1) and IKKɛ. In this study we attempted to elucidate the molecular mechanism(s) underlying the inhibition of BX-795 on Herpes simplex virus (HSV) replication. HEC-1-A or Vero cells were treated with BX-795 and infected with HSV-1 or HSV-2 for different periods. BX-795 (3.125-25 μmol/L) dose-dependently suppressed HSV-2 replication, and displayed a low cytotoxicity to the host cells...
March 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/27959381/nobiletin-a-citrus-flavonoid-activates-vasodilator-stimulated-phosphoprotein-in-human-platelets-through-non-cyclic-nucleotide-related-mechanisms
#19
Thanasekaran Jayakumar, Kao-Chang Lin, Wan-Jung Lu, Chia-Ying Lin, Geraldine Pitchairaj, Jiun-Yi Li, Joen-Rong Sheu
Nobiletin, a bioactive polymethoxylated flavone, has been described to possess a diversity of biological effects through its antioxidant and anti-inflammatory properties. Vasodilator-stimulated phosphoprotein (VASP) is a common substrate for cyclic AMP and cyclic GMP-regulated protein kinases [i.e., cyclic AMP-dependent protein kinase (PKA; also known as protein kinase A) and cyclic GMP-dependent protein kinase (PKG; also known as protein kinase G)] and it has been shown to be directly phosphorylated by protein kinase C (PKC)...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27743232/induction-of-nrf2-mediated-phase-ii-detoxifying-antioxidant-enzymes-in-vitro-by-chitosan-caffeic-acid-against-hydrogen-peroxide-induced-hepatotoxicity-through-jnk-erk-pathway
#20
Chang-Bum Ahn, Jae-Young Je, Young-Sang Kim, Sun-Joo Park, Boo Il Kim
Chemical modification of chitosan is a promising method for the improvement of biological activity. In this study, chitosan-caffeic acid (CCA) was prepared and its in vitro hepatoprotective ability against hydrogen peroxide-induced hepatic damage in liver cells was evaluated. Treatment with CCA (50-400 µg/mL) did not show cytotoxicity and also significantly (p < 0.05) recovered cell viability against 650 µM hydrogen peroxide-induced hepatotoxicity. CCA treatment attenuated reactive oxygen species generation and lipid peroxidation in addition to increasing cellular glutathione level in cultured hepatocytes...
January 2017: Molecular and Cellular Biochemistry
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