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SP600125 PD98059 SB203580

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https://www.readbyqxmd.com/read/29770357/mapk-pathway-involved-in-epidermal-terminal-differentiation-of-normal-human-epidermal-keratinocytes
#1
Xianguang Meng, Liyun Qiu, Haiyan Song, Ningning Dang
Objective: To investigate the effect of mitogen-activated protein kinase (MAPK) signaling pathway in epidermal terminal differentiation. Methods: The MAPK pathways (p38, ERK1/2, JNK) were inhibited by SB203580, PD98059, and SP600125 in normal human epidermal keratinocytes (NHEKs), respectively. Western blotting assays were performed to detect expression of filaggrin and differentiation-related proteins. The mRNA expressions of differentiation-related proteins were detected by real-time quantitative PCR (qRT-PCR)...
2018: Open Medicine (Warsaw, Poland)
https://www.readbyqxmd.com/read/29745021/the-rhoa-rock-pathway-mediates-high-glucose-induced-cardiomyocyte-apoptosis-via-oxidative-stress-jnk-and-p38mapk-pathways-in-neonatal-rats
#2
Hong Zhou, Yonghong Sun, Lihui Zhang, Wenyuan Kang, Na Li, Yongjun Li
AIMS: To understand the roles of the RhoA/ROCK and MAPK pathways in high glucose (HG)-induced apoptosis and oxidative stress in cardiomyocytes. MATERIALS AND METHODS: Neonatal rat cardiomyocytes were cultured in DMEM, supplemented with 5.5 or 30 mmol/L D-glucose, in the presence or absence of fasudil (50 or 100 μM), SB203580, SP600125 or PD98059 (10 μM, respectively). The percentage of early apoptotic cardiomyocytes was evaluated using flow cytometry. The SOD activity and MDA contents in the cellular supernatants were measured...
May 9, 2018: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/29723849/the-mechanism-of-action-of-zingerone-in-the-pacemaker-potentials-of-interstitial-cells-of-cajal-isolated-from-murine-small-intestine
#3
Jung Nam Kim, Hyun Jung Kim, Iksung Kim, Yun Tai Kim, Byung Joo Kim
BACKGROUND/AIMS: Zingerone, a major component found in ginger root, is clinically effective for the treatment of various diseases. Interstitial cells of Cajal (ICCs) are the pacemaker cells responsible for slow waves in the gastrointestinal (GI) tract. We investigated the effects of zingerone on the pacemaker potentials of ICCs to assess its mechanisms of action and its potential as a treatment for GI tract motility disorder. METHODS: We isolated ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record the pacemaker potentials in cultured ICCs...
April 28, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29627692/hemin-blunts-the-depressant-effect-of-chronic-nicotine-on-reflex-tachycardia-via-activation-of-central-nos-pi3k-pathway-in-female-rats
#4
Mohamed A Fouda, Hanan M El-Gowelli, Sahar M El-Gowilly, Mahmoud M El-Mas
BACKGROUND: Chronic nicotine administration impairs reflex chronotropic responses that follow arterial baroreceptor unloading in female rats with repleted, but not depleted (ovariectomized, OVX), estrogen (E2 ). This study investigated whether products of nitric oxide synthase (NOS) and/or heme oxygenase (HO) and related soluble guanylate cyclase (sGC)/phosphatidylinositol 3-kinase (PI3K)/mitogen-activated protein kinases (MAPKs) signaling mediate the E2 -sensitive depressant effect of nicotine on reflex tachycardia...
October 3, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29559303/remifentanil-preconditioning-confers-cardioprotection-via-c-jun-nh-2-terminal-kinases-and-extracellular-signal-regulated-kinases-pathways-in-ex-vivo-failing-rat-heart
#5
Shi-Yun Jin, Jun Huang, Hai-Juan Zhu, Hao Wu, Shi-Jin Xu, Michael G Irwin, Shu-Fang He, Ye Zhang
Remifentanil preconditioning (RPC) exerts protection in normal hearts, but has not been investigated in heart failure. The aim of the present study was to evaluate the effect of RPC in a chronic failing rat heart model and the mechanisms involving mitogen-activated protein kinases (MAPK) and Bcl-2 protein family. The doxorubicin induced failing rat hearts were subjected to 30min ischemia / 120min reperfusion (IR) with or without RPC by using Langendorff apparatus. RPC was induced by three cycles of 5min remifentanil / 5min drug-free perfusion before IR, with three different concentrations: 25, 50 and 100μg/l...
March 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29498687/wedelolactone-enhances-osteoblastogenesis-through-erk-and-jnk-mediated-bmp2-expression-and-smad-1-5-8-phosphorylation
#6
Di Zhu, Xue Deng, Xiao-Fei Han, Xiao-Xin Sun, Tao-Wen Pan, Lu-Ping Zheng, Yan-Qiu Liu
Our previous study showed that wedelolactone, a compound isolated from Ecliptae herba , has the potential to enhance osteoblastogenesis. However, the molecular mechanisms by which wedelolactone promoted osteoblastogenesis from bone marrow mesenchymal stem cells (BMSCs) remain largely unknown. In this study, treatment with wedelolactone (2 μg/mL) for 3, 6, and 9 days resulted in an increase in phosphorylation of extracellular signal-regulated kinases (ERKs), c-Jun N -terminal protein kinase (JNK), and p38. Phosphorylation of mitogen-activated protein kinases (MAPKs), ERK and JNK started to increase on day 3 of treatment, and p38 phosphorylation was increased by day 6 of treatment...
March 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29400080/chronic-intermittent-hypobaric-hypoxia-provides-vascular-protection-in-aorta-of-two-kidney-one-clip-rat-model-of-hypertension
#7
Fu Congrui, Li Na, Yuan Yujia, Wang Ri, Chen Jinting, Yang Jing, Guo Zan, Wang Sheng, Zhang Yi, Liu Yixian, Dong Jinghui
Many studies have demonstrated that chronic intermittent hypobaric hypoxia (CIHH) can reduce blood pressure in spontaneously hypertensive rats and renovascular hypertensive (RVH) rats in which endothelial dysfunction is determined as a critical factor. However, whether CIHH can regulate vasodilation of the aorta in RVH rats remains unknown. The purpose of this study was to investigate the effect of CIHH on impaired relaxation of the aorta in the two-kidney one-clip (2K1C) RVH rat model. The results showed CIHH improved the impaired endothelium-dependent relaxation in the 2K1C rat aorta...
February 3, 2018: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29387240/p38-mapk-mk2-pathway-regulates-the-heat-stress-induced-accumulation-of-reactive-oxygen-species-that-mediates-apoptotic-cell-death-in-glial-cells
#8
Hongbo Li, Yanan Liu, Zhengtao Gu, Li Li, Yunsong Liu, Lin Wang, Lei Su
Previous studies have demonstratedf that heat stress can induce injury of the central nervous system and lead to neuronal cell apoptosis. However, the molecular mechanisms underlying these cellular changes remain unclear. In the present study, flow cytometry was used to investigate heat-stress-induced apoptosis, and caspase-3 activation was also assessed in neurons. The role of reactive oxygen species (ROS) accumulation in the heat-stress-induced apoptosis of neurons was demonstrated using the antioxidant drug manganese (III) tetrakis (4-benzoic acid)porphyrin...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29285098/aspirin-suppresses-tnf-%C3%AE-induced-mmp-9-expression-via-nf-%C3%AE%C2%BAb-and-mapk-signaling-pathways-in-raw264-7-cells
#9
Ping Zhang, Chao Wu, Xiao-Hui Huang, Chen-Lin Shen, Lin Li, Wei Zhang, Cheng-Zeng Yao
Numerous studies have indicated that the expression of matrix metalloproteinase-9 (MMP-9) contributes to the atherosclerotic plaque hemorrhage and rupture. Aspirin, a non-steroidal anti-inflammation drug, has been known for its anti-platelet effect in the prevention of the vascular complications of atherosclerosis. The present study aimed to investigate the pharmacological effects of aspirin on tumor necrosis factor-α (TNF-α)-induced MMP-9 expression and the underlying molecular mechanisms in murine macrophage RAW264...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29130959/ginsenoside-rg1-protects-cardiomyocytes-against-hypoxia-reoxygenation-injury-via-activation-of-nrf2-ho-1-signaling-and-inhibition-of-jnk
#10
Qianhui Li, Yin Xiang, Yu Chen, Yong Tang, Yachen Zhang
BACKGROUND/AIMS: Excessive reactive oxygen species (ROS) disturb the physiology of H9c2 cells, which is regarded as a major cause of H9c2 cardiomyocyte apoptosis. Ginsenoside Rg1 is the main active extract of ginseng, which has important antioxidant properties in various cell models. This project investigated the role of ginsenoside Rg1 in hypoxia/reoxygenation (H/R)-induced oxidative stress injury in cultured H9c2 cells to reveal the underlying signaling pathways. METHODS: H9c2 cells were pretreated with ginsenoside Rg1 for 12 h before exposure to H/R...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29019920/pratol-an-o-methylated-flavone-induces-melanogenesis-in-b16f10-melanoma-cells-via-p-p38-and-p-jnk-upregulation
#11
You Chul Chung, Seoyeon Kim, Jin Hwa Kim, Geun Soo Lee, Jung No Lee, Nam Ho Lee, Chang-Gu Hyun
Tyrosinase is the rate-limiting enzyme critical for melanin synthesis. It controls pigmentation in the skin. Activation of tyrosinase is currently the most common approach in the development of tanning and haircare products. Pratol is a 7-hydroxy-4-methoxyflavone found in Trifoliumpratense. In this study, we investigated the effects of pratol on melanogenesis. We also studied the mechanism of action of pratol in B16F10 mouse melanoma cells. The cells were treated with various concentrations (6.25, 12.5, 25, and 50 μM) of pratol to observe its effects...
October 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29018002/glucose-uptake-inhibition-decreases-expressions-of-receptor-activator-of-nuclear-factor-kappa-b-ligand-rankl-and-osteocalcin-in-osteocytic-mlo-y4-a2-cells
#12
Ayumu Takeno, Ippei Kanazawa, Masakazu Notsu, Ken-Ichiro Tanaka, Toshitsugu Sugimoto
Bone and glucose metabolism are closely associated with each other. Both osteoblast and osteoclast functions are important for the action of osteocalcin, which plays pivotal roles as an endocrine hormone regulating glucose metabolism. However, it is unknown whether osteocytes are involved in the interaction between bone and glucose metabolism. We used MLO-Y4-A2, a murine long bone-derived osteocytic cell line, to investigate effects of glucose uptake inhibition on expressions of osteocalcin and bone-remodeling modulators in osteocytes...
February 1, 2018: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28968596/resveratrol-inhibits-the-epidermal-growth-factor-induced-migration-of-osteoblasts-the-suppression-of-sapk-jnk-and-akt
#13
Tetsu Kawabata, Haruhiko Tokuda, Kazuhiko Fujita, Shingo Kainuma, Go Sakai, Rie Matsushima-Nishiwaki, Osamu Kozawa, Takanobu Otsuka
BACKGROUND/AIMS: Resveratrol is a polyphenol enriched in the skins of grapes and berries, that shows various beneficial effects for human health. In the present study, we investigated the mechanism behind the epidermal growth factor (EGF)-induced migration of osteoblast-like MC3T3-E1 cells, and the effect of resveratrol on this cell migration. METHODS: The cell migration was examined using Boyden chamber, and phosphorylation of each kinase was analyzed by Western blotting...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28951356/-expressions-of-inflammatory-cytokines-in-mouse-peritoneal-macrophages-induced-by-mrp8-mrp14-in-vitro
#14
Juan Wang, Lei Li, Hai-Hua Luo, Yong Jiang
OBJECTIVE: To investigate MRP8/MRP14-induced expression of inflammatory cytokines in mouse peritoneal macrophages and explore the mechanism. METHODS: Tumor necrosis factor-α (TNF-α), interferon-γ inducible protein 10 (IP-10), interleukin-2 (IL-2), IL-6, IL-5 and interferon-γ (IFN-γ) proteins in the culture supernatants of mouse peritoneal macrophages treated with recombinant MRP8/MRP14 were quantified using Luminex xMAP system. TNF-α, IP-10 and IL-6 levels were detected in the culture supernatants of the peritoneal macrophages after treatment with different domains of MRP14...
September 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28902366/heat-shock-protein-27-hspb1-suppresses-the-pdgf-bb-induced-migration-of-osteoblasts
#15
Shingo Kainuma, Haruhiko Tokuda, Naohiro Yamamoto, Gen Kuroyanagi, Kazuhiko Fujita, Tetsu Kawabata, Go Sakai, Rie Matsushima-Nishiwaki, Osamu Kozawa, Takanobu Otsuka
Heat shock protein 27 (HSP27/HSPB1), one of the small heat shock proteins, is constitutively expressed in various tissues. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor‑BB (PDGF‑BB)‑stimulated migration of osteoblast-like MC3T3-E1 cells. PDGF-BB by itself barely upregulated the expression of HSP27 protein, but stimulated the phosphorylation of HSP27 in these cells...
October 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28878808/luteolin-quercetin-genistein-and-quercetagetin-inhibit-the-effects-of-lipopolysaccharide-obtained-from-porphyromonas-gingivalis-in-h9c2-cardiomyoblasts
#16
Gloria Gutiérrez-Venegas, Alfredo Torras-Ceballos, Juan Arturo Gómez-Mora, Berenice Fernández-Rojas
BACKGROUND: One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from P. gingivalis promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2)...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28842382/varenicline-promotes-endothelial-cell-migration-by-lowering-vascular-endothelial-cadherin-levels-via-the-activated-%C3%AE-7-nicotinic-acetylcholine-receptor-mitogen-activated-protein-kinase-axis
#17
Mitsuhisa Koga, Yuki Kanaoka, Keita Sugiyama, Kaoru Ohishi, Yuka Ejima, Mami Hisanaga, Yasufumi Kataoka, Atsushi Yamauchi
Varenicline is a widely used and effective drug for smoking cessation. Despite its efficacy, varenicline increases the risk of cardiovascular disease. We previously demonstrated that varenicline aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice. However, little is known about its effects in vascular endothelial cells. Therefore, we examined whether varenicline promotes migration of human umbilical vein endothelial cells (HUVECs) using the Boyden chamber assay. Varenicline (100μM) markedly promoted migration of HUVECs and decreased expression of vascular endothelial (VE)-cadherin, an endothelial adhesion molecule...
September 1, 2017: Toxicology
https://www.readbyqxmd.com/read/28656249/oxidative-stress-regulates-mitogen%C3%A2-activated-protein-kinases-and-c%C3%A2-jun-activation-involved-in-heat-stress-and-lipopolysaccharide%C3%A2-induced-intestinal-epithelial-cell-apoptosis
#18
Yanan Liu, Zhenglian Wang, Weidang Xie, Zhengtao Gu, Qiulin Xu, Lei Su
Heat stress and gut‑derived endotoxinemia are common causes of multiple organ dysfunction syndrome in heat stroke patients. Evidence has demonstrated that cell apoptosis in the small intestine serves an important role in the pathogenesis of heatstroke, which leads to increased intestinal permeability to endotoxin or lipopolysaccharides (LPS) from the gut entering the circulation. However, little is known about the potential underlying mechanisms mediating heat stress combined with LPS‑induced intestinal epithelial cell apoptosis...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627696/desipramine-induces-apoptosis-in-hepatocellular-carcinoma-cells
#19
Dong Kwon Yang, Shang-Jin Kim
Antitumor effects of antidepressants have been reported in many cancer cell lines. However, anti-proliferative effects of desipramine, a tricyclic antidepressant, in hepatocellular carcinoma are currently unknown. In this study, we examined the effects of desipramine in human hepatoma Hep3B cells. To evaluate anti-proliferative effects of desipramine in Hep3B cells, we determined cell viability, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), mitogen-activated protein kinase (MAPK) activity, and intracellular Ca2+ levels after desipramine treatment...
June 15, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28601021/aspergillus-fumigatus-induced-early-inflammatory-response-in-pulmonary-microvascular-endothelial-cells-role-of-p38-mapk-and-inhibition-by-silibinin
#20
Jun Song, Weihua Pan, Yue Sun, Jing Han, Weimin Shi, Wanqing Liao
Human invasive pulmonary aspergillosis (IPA) is a serious infectious disease mainly caused by Aspergillus fumigatus (A. fumigatus). Pulmonary microvascular endothelial cells (PMVECs) are important ones in the human lung tissue. However, it remains unclear about the role of PMVECs in IPA. In the present study, we cocultured PMVECs with A. fumigatus. We observed that A. fumigatus induced dose- and time-dependent increases of interleukin 6 (IL-6), interleukin 1β (IL-1β) and intercellular adhesion molecule 1 (ICAM-1) concentration in the cultures...
June 7, 2017: International Immunopharmacology
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