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https://www.readbyqxmd.com/read/28902366/heat-shock-protein-27-hspb1-suppresses-the-pdgf-bb-induced-migration-of-osteoblasts
#1
Shingo Kainuma, Haruhiko Tokuda, Naohiro Yamamoto, Gen Kuroyanagi, Kazuhiko Fujita, Tetsu Kawabata, Go Sakai, Rie Matsushima-Nishiwaki, Osamu Kozawa, Takanobu Otsuka
Heat shock protein 27 (HSP27/HSPB1), one of the small heat shock proteins, is constitutively expressed in various tissues. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor‑BB (PDGF‑BB)‑stimulated migration of osteoblast-like MC3T3-E1 cells. PDGF-BB by itself barely upregulated the expression of HSP27 protein, but stimulated the phosphorylation of HSP27 in these cells...
October 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28878808/luteolin-quercetin-genistein-and-quercetagetin-inhibit-the-effects-of-lipopolysaccharide-obtained-from-porphyromonas-gingivalis-in-h9c2-cardiomyoblasts
#2
Gloria Gutiérrez-Venegas, Alfredo Torras-Ceballos, Juan Arturo Gómez-Mora, Berenice Fernández-Rojas
BACKGROUND: One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is Porphyromonas gingivalis. It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from P. gingivalis promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2)...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28842382/varenicline-promotes-endothelial-cell-migration-by-lowering-vascular-endothelial-cadherin-levels-via-the-activated-%C3%AE-7-nicotinic-acetylcholine-receptor-mitogen-activated-protein-kinase-axis
#3
Mitsuhisa Koga, Yuki Kanaoka, Keita Sugiyama, Kaoru Ohishi, Yuka Ejima, Mami Hisanaga, Yasufumi Kataoka, Atsushi Yamauchi
Varenicline is a widely used and effective drug for smoking cessation. Despite its efficacy, varenicline increases the risk of cardiovascular disease. We previously demonstrated that varenicline aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice. However, little is known about its effects in vascular endothelial cells. Therefore, we examined whether varenicline promotes migration of human umbilical vein endothelial cells (HUVECs) using the Boyden chamber assay. Varenicline (100μM) markedly promoted migration of HUVECs and decreased expression of vascular endothelial (VE)-cadherin, an endothelial adhesion molecule...
August 24, 2017: Toxicology
https://www.readbyqxmd.com/read/28656249/oxidative-stress-regulates-mitogen%C3%A2-activated-protein-kinases-and-c%C3%A2-jun-activation-involved-in-heat-stress-and-lipopolysaccharide%C3%A2-induced-intestinal-epithelial-cell-apoptosis
#4
Yanan Liu, Zhenglian Wang, Weidang Xie, Zhengtao Gu, Qiulin Xu, Lei Su
Heat stress and gut‑derived endotoxinemia are common causes of multiple organ dysfunction syndrome in heat stroke patients. Evidence has demonstrated that cell apoptosis in the small intestine serves an important role in the pathogenesis of heatstroke, which leads to increased intestinal permeability to endotoxin or lipopolysaccharides (LPS) from the gut entering the circulation. However, little is known about the potential underlying mechanisms mediating heat stress combined with LPS‑induced intestinal epithelial cell apoptosis...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627696/desipramine-induces-apoptosis-in-hepatocellular-carcinoma-cells
#5
Dong Kwon Yang, Shang-Jin Kim
Antitumor effects of antidepressants have been reported in many cancer cell lines. However, anti-proliferative effects of desipramine, a tricyclic antidepressant, in hepatocellular carcinoma are currently unknown. In this study, we examined the effects of desipramine in human hepatoma Hep3B cells. To evaluate anti-proliferative effects of desipramine in Hep3B cells, we determined cell viability, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), mitogen-activated protein kinase (MAPK) activity, and intracellular Ca2+ levels after desipramine treatment...
June 15, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28601021/aspergillus-fumigatus-induced-early-inflammatory-response-in-pulmonary-microvascular-endothelial-cells-role-of-p38-mapk-and-inhibition-by-silibinin
#6
Jun Song, Weihua Pan, Yue Sun, Jing Han, Weimin Shi, Wanqing Liao
Human invasive pulmonary aspergillosis (IPA) is a serious infectious disease mainly caused by Aspergillus fumigatus (A. fumigatus). Pulmonary microvascular endothelial cells (PMVECs) are important ones in the human lung tissue. However, it remains unclear about the role of PMVECs in IPA. In the present study, we cocultured PMVECs with A. fumigatus. We observed that A. fumigatus induced dose- and time-dependent increases of interleukin 6 (IL-6), interleukin 1β (IL-1β) and intercellular adhesion molecule 1 (ICAM-1) concentration in the cultures...
June 7, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28598073/-effects-of-fgf-21-on-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-in-high-glucose-environment
#7
Xi Li, Cheng Chen, Zhen-Mei An, Yu-Jue Li, Mei Zhang, He He, Shuang-Qing Li
OBJECTIVES: To determine the effect of fibroblast growth factor-21(FGF-21)on the osteogenic differention of human bone mesenchymal stem cells (hBMSCs) exposed to a hyperglycemia condition in vitro. METHODS: hBMSCs were isolated from adult bone marrows, and identified by Alizarin red and oil red O staining. The expressions of immunophenotype were analysed using flow cytometry (CD105, CD90, CD73, CD44).HBMSCs were divided into control group[glucose (Glu) concentration of 5...
September 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28494003/p2y6-receptors-are-involved-in-mediating-the-effect-of-inactivated-avian-influenza-virus-h5n1-on-il-6-cxcl8-mrna-expression-in-respiratory-epithelium
#8
Nawiya Huipao, Suparerk Borwornpinyo, Suwimon Wiboon-Ut, Craig R Campbell, Il-Ha Lee, Siriphun Hiranyachattada, Chonlaphat Sukasem, Arunee Thitithanyanont, Chumpol Pholpramool, David I Cook, Anuwat Dinudom
One of the key pathophysiologies of H5N1 infection is excessive proinflammatory cytokine response (cytokine storm) characterized by increases in IFN-β, TNF-α, IL-6, CXCL10, CCL4, CCL2 and CCL5 in the respiratory tract. H5N1-induced cytokine release can occur via an infection-independent mechanism, however, detail of the cellular signaling involved is poorly understood. To elucidate this mechanism, the effect of inactivated (β-propiolactone-treated) H5N1 on the cytokine and chemokine mRNA expression in 16HBE14o- human respiratory epithelial cells was investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28437005/convergent-erk1-2-p38-and-jnk-mitogen-activated-protein-kinases-mapks-signalling-mediate-catecholoestradiol-induced-proliferation-of-ovine-uterine-artery-endothelial-cells
#9
Rosalina Villalon Landeros, Sheikh O Jobe, Gabrielle Aranda-Pino, Gladys E Lopez, Jing Zheng, Ronald R Magness
KEY POINTS: The catechol metabolites of 17β-oestradiol (E2 β), 2-hydroxyoestradiol (2-OHE2 ) and 4-hydroxyoestradiol (4-OHE2 ), stimulate proliferation of pregnancy-derived ovine uterine artery endothelial cells (P-UAECs) through β-adrenoceptors (β-ARs) and independently of the classic oestrogen receptors (ERs). Herein we show that activation of ERK1/2, p38 and JNK mitogen activated protein kinases (MAPKs) is necessary for 2-OHE2 - and 4-OHE2 -induced P-UAEC proliferation, as well as proliferation induced by the parent hormone E2 β and other β-AR signalling hormones (i...
July 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28316375/torilin-inhibits-inflammation-by-limiting-tak1-mediated-map-kinase-and-nf-%C3%AE%C2%BAb-activation
#10
Mehari Endale, Tae-Hwan Kim, Yi-Seong Kwak, Na-Mi Kim, Seung-Hyung Kim, Jae Youl Cho, Bong-Sik Yun, Man-Hee Rhee
Torilin, a sesquiterpene isolated from the fruits of Torilis japonica, has shown antimicrobial, anticancer, and anti-inflammatory properties. However, data on the mechanism of torilin action against inflammation is limited. This study aimed at determining the anti-inflammatory property of torilin in LPS-induced inflammation using in vitro model of inflammation. We examined torilin's effect on expression levels of inflammatory mediators and cytokines in LPS-stimulated RAW 264.7 macrophages. The involvement of NF-kB and AP-1, MAP kinases, and adaptor proteins were assessed...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28123510/effects-of-prunus-mume-siebold-zucc-in-the-pacemaking-activity-of-interstitial-cells-of-cajal-in-murine-small-intestine
#11
Sang Weon Lee, Sung Jin Kim, Hyungwoo Kim, Dongki Yang, Hyun Jung Kim, Byung Joo Kim
Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28115173/cyclosporine-counteracts-endotoxemia-evoked-reductions-in-blood-pressure-and-cardiac-autonomic-dysfunction-via-central-sgc-mapks-signaling-in-rats
#12
Marwa Y Sallam, Sahar M El-Gowilly, Abdel-Galil A Abdel-Galil, Mahmoud M El-Mas
The immunosuppressant drug cyclosporine A (CSA) improves survivability in endotoxemia and offsets associated loss in vascular reactivity and hypotension. We tested the hypothesis that central phosphoinositide-3-kinase (PI3K)/soluble guanylate cyclase (sGC)/mitogen activated protein kinases (MAPKs) cascade modulates the CSA counteraction of endotoxic hypotension and cardiac autonomic dysfunction. The effects of pharmacologic inhibition of these molecular substrates in central pools on CSA interaction with cardiovascular responses evoked by lipopolysaccharide (LPS) were evaluated in conscious rats...
February 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28112176/bx-795-inhibits-hsv-1-and-hsv-2-replication-by-blocking-the-jnk-p38-pathways-without-interfering-with-pdk1-activity-in-host-cells
#13
Ai-Rong Su, Min Qiu, Yan-Lei Li, Wen-Tao Xu, Si-Wei Song, Xiao-Hui Wang, Hong-Yong Song, Nan Zheng, Zhi-Wei Wu
BX-795 is an inhibitor of 3-phosphoinositide-dependent kinase 1 (PDK1), but also a potent inhibitor of the IKK-related kinase, TANKbinding kinase 1 (TBK1) and IKKɛ. In this study we attempted to elucidate the molecular mechanism(s) underlying the inhibition of BX-795 on Herpes simplex virus (HSV) replication. HEC-1-A or Vero cells were treated with BX-795 and infected with HSV-1 or HSV-2 for different periods. BX-795 (3.125-25 μmol/L) dose-dependently suppressed HSV-2 replication, and displayed a low cytotoxicity to the host cells...
March 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/27959381/nobiletin-a-citrus-flavonoid-activates-vasodilator-stimulated-phosphoprotein-in-human-platelets-through-non-cyclic-nucleotide-related-mechanisms
#14
Thanasekaran Jayakumar, Kao-Chang Lin, Wan-Jung Lu, Chia-Ying Lin, Geraldine Pitchairaj, Jiun-Yi Li, Joen-Rong Sheu
Nobiletin, a bioactive polymethoxylated flavone, has been described to possess a diversity of biological effects through its antioxidant and anti-inflammatory properties. Vasodilator-stimulated phosphoprotein (VASP) is a common substrate for cyclic AMP and cyclic GMP-regulated protein kinases [i.e., cyclic AMP-dependent protein kinase (PKA; also known as protein kinase A) and cyclic GMP-dependent protein kinase (PKG; also known as protein kinase G)] and it has been shown to be directly phosphorylated by protein kinase C (PKC)...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27743232/induction-of-nrf2-mediated-phase-ii-detoxifying-antioxidant-enzymes-in-vitro-by-chitosan-caffeic-acid-against-hydrogen-peroxide-induced-hepatotoxicity-through-jnk-erk-pathway
#15
Chang-Bum Ahn, Jae-Young Je, Young-Sang Kim, Sun-Joo Park, Boo Il Kim
Chemical modification of chitosan is a promising method for the improvement of biological activity. In this study, chitosan-caffeic acid (CCA) was prepared and its in vitro hepatoprotective ability against hydrogen peroxide-induced hepatic damage in liver cells was evaluated. Treatment with CCA (50-400 µg/mL) did not show cytotoxicity and also significantly (p < 0.05) recovered cell viability against 650 µM hydrogen peroxide-induced hepatotoxicity. CCA treatment attenuated reactive oxygen species generation and lipid peroxidation in addition to increasing cellular glutathione level in cultured hepatocytes...
January 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27671463/interaction-between-lps-and-a-dental-resin-monomer-on-cell-viability-in-mouse-macrophages
#16
Helmut Schweikl, Matthias Widbiller, Stephanie Krifka, Johannes Klement, Christine Petzel, Carola Bolay, Karl-Anton Hiller, Wolfgang Buchalla
OBJECTIVE: Lipopolysaccharide (LPS) from cariogenic microorganisms and resin monomers like HEMA (2-hydroxyethyl methacrylate) included in dentin adhesive are present in a clinical situation in deep dentinal cavity preparations. Here, cell survival, expression of proteins related to redox homeostasis, and viability of mouse macrophages exposed to LPS and HEMA were analyzed with respect to the influence of oxidative stress. METHODS: Cell survival of RAW264.7 mouse macrophages was determined using a crystal violet assay, protein expression was detected by Western blotting, and HEMA- or LPS-induced apoptosis (cell viability) was analyzed by flow cytometry...
December 2016: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/27573189/collagen-regulates-transforming-growth-factor-%C3%AE-receptors-of-hl-1-cardiomyocytes-through-activation-of-stretch-and-integrin-signaling
#17
Yen-Yu Lu, Yung-Kuo Lin, Yu-Hsun Kao, Cheng-Chih Chung, Yung-Hsin Yeh, Shih-Ann Chen, Yi-Jen Chen
The extracellular matrix (ECM) and transforming growth factor-β (TGF)-β are important in cardiac fibrosis, however, the effects of the ECM on TGF‑β signaling remain to be fully elucidated. The aims of the present study were to evaluate the role of collagen in TGF‑β signaling and examine the underlying mechanisms. In the present study, western blot analysis was used to examine TGF‑β signaling in HL‑1 cells treated with and without (control) type I collagen (10 µg/ml), which was co‑administered with either an anti‑β1 integrin antibody (10 µg/ml) or a stretch‑activated channel inhibitor (gadolinium; 50 µM)...
October 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27514378/lipopolysaccharide-induces-sbd-1-expression-via-the-p38-mapk-signaling-pathway-in-ovine-oviduct-epithelial-cells
#18
Qi Li, Fuxiang Bao, Dafu Zhi, Moning Liu, Qin Yan, Xinxin Zheng, Lixin Ren, Shan Cong, Yan Li, Guifang Cao
BACKGROUND: Beta defensins are secreted from ovine oviduct epithelial cells (OOECs) in response to microbial infection, and are potential alternatives to antibiotic agents in the treatment of microorganism infection, particularly given the abuse of antibiotic agents and the increasing number of drug-resistant bacteria. The aberrant expression of defensins may result in disorders involving organ and oviduct inflammation, such as salpingitis. METHODS: In the present study, we investigated the effects of LPS on the mRNA expression levels of sheep β-defensin-1 (SBD-1) in ovine oviduct epithelial cells...
August 11, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27507784/activation-of-p38-mitogen-activated-protein-kinase-by-clotrimazole-induces-multidrug-resistance-associated-protein-3-activation-through-a-novel-transcriptional-element
#19
Takamitsu Sasaki, Keita Inami, Yoshihiro Numata, Kodai Funakoshi, Midori Yoshida, Takeshi Kumagai, Shuichi Kanno, Satomi Matsui, Takayoshi Toriyabe, Yasushi Yamazoe, Kouichi Yoshinari, Kiyoshi Nagata
Multidrug resistance-associated protein 3 (MRP3) is a basolaterally localized transporter in the liver and contributes to the transport of various metabolites such as conjugates of endogenous compounds and drugs from hepatocytes. MRP3 expression in the human liver is low under normal physiologic conditions but is induced by drug treatment. Although several studies have identified a region necessary for the basal transcription of MRP3, no region that responds to drugs has been reported. To identify the xenobiotic-responsive elements of MRP3, we constructed a luciferase reporter plasmid containing the MRP3 5'-flanking region up to -10 kb upstream from the transcription start site...
October 2016: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/27439062/c-jun-n-terminal-kinase-mediates-prostaglandin-induced-sympathoexcitation-in-rats-with-chronic-heart-failure-by-reducing-gad1-and-gabra1-expression
#20
R Wang, W Zhang, Z Dong, Y Qi, M Hultström, X Zhou, E Y Lai
AIM: Prostaglandin E2 mediates sympathoexcitation in chronic heart failure (CHF) through EP3 receptors (PTGER3) in the paraventricular nucleus (PVN). The aim of this study was to investigate the role of c-Jun N-terminal kinase (JNK) in expressional regulation of gamma-aminobutyric acid signalling in PVN in CHF rats. METHODS: Chronic heart failure was induced by left coronary ligation in Wistar rats. Renal sympathetic nerve discharge (RSND) and mean arterial pressure (MAP) responses to the PVN infusion were determined in anaesthetized rats...
July 20, 2016: Acta Physiologica
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