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Flt 1

Veit Rothhammer, Davis M Borucki, Emily C Tjon, Maisa C Takenaka, Chun-Cheih Chao, Alberto Ardura-Fabregat, Kalil Alves de Lima, Cristina Gutiérrez-Vázquez, Patrick Hewson, Ori Staszewski, Manon Blain, Luke Healy, Tradite Neziraj, Matilde Borio, Michael Wheeler, Loic Lionel Dragin, David A Laplaud, Jack Antel, Jorge Ivan Alvarez, Marco Prinz, Francisco J Quintana
Microglia and astrocytes modulate inflammation and neurodegeneration in the central nervous system (CNS)1-3 . Microglia modulate pro-inflammatory and neurotoxic activities in astrocytes, but the mechanisms involved are not completely understood4,5 . Here we report that TGFα and VEGF-B produced by microglia regulate the pathogenic activities of astrocytes in the experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis. Microglia-derived TGFα acts via the ErbB1 receptor in astrocytes to limit their pathogenic activities and EAE development...
May 16, 2018: Nature
Xiuan Xu, Yuling Yan, Fang Liu, Lina Wu, Mengping Shao, Kai Wang, Xilin Sun, Yingbo Li, Ernest Sanyare Warmann Beinpuo, Baozhong Shen
BACKGROUND: Folate receptors (FRs) hold great potential as important diagnostic and prognostic biological marker for cancer. PURPOSE: To assess the targeted capability of the FR-targeted perfluorocarbon (PFC) nanoparticles and to assess in vivo the relationship between FR expression and tumor proliferation with fluorine-19 (19 F) MR molecular imaging. STUDY TYPE: Prospective animal cancer model. ANIMAL MODEL: H460 (n = 14) and A549 (n = 14) nude mice subcutaneous tumor models...
May 13, 2018: Journal of Magnetic Resonance Imaging: JMRI
Michiko Yamashita, Keiichi Kumasawa, Hitomi Nakamura, Tadashi Kimura
No abstract text is available yet for this article.
May 9, 2018: Biochemical and Biophysical Research Communications
Fabio Cattaneo, Martina Castaldo, Melania Parisi, Raffaella Faraonio, Gabriella Esposito, Rosario Ammendola
In the vasculature, NADPH oxidase is the main contributor of reactive oxygen species (ROS) which play a key role in endothelial signalling and functions. We demonstrate that ECV304 cells express p47phox , p67phox , and p22phox subunits of NADPH oxidase, as well as formyl peptide receptors 1 and 3 (FPR1/3), which are members of the GPCR family. By RT-PCR, we also detected Flt-1 and Flk-1/KDR in these cells. Stimulation of FPR1 by N-fMLP induces p47phox phosphorylation, which is the crucial event for NADPH oxidase-dependent superoxide production...
2018: Oxidative Medicine and Cellular Longevity
Takayasu Ohtake, Shuzo Kobayashi, Shimon Slavin, Yasuhiro Mochida, Kunihiro Ishioka, Hidekazu Moriya, Sumi Hidaka, Ryo Matsuura, Maki Sumida, Daisuke Katagiri, Eisei Noiri, Kayoko Okada, Hiroshi Mizuno, Rica Tanaka
Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mice aged 7 to 8 wk. PBMNCs were isolated from healthy volunteers and were subjected to quality and quantity controlled (QQc) culture for 7 d in medium containing stem cell factor, thrombopoietin, Flt-3 ligand, vascular endothelial growth factor, and interleukin 6...
January 1, 2018: Cell Transplantation
Mahmoud Ali Abdelaziz Mahmoud, Rafat Milad Mohareb, Mashari Ahmed Alshereef
BACKGROUND: Among wide range of heterocyclic steroidal derivatives acquired a special attention due to their wide range of pharmacological activities especially the therapeutic activities. Many pharmacological drugs containing the steroid nucleus were known in the market. OBJECTIVE: Our main aim of this work was to synthesis a seies of heterocyclic compounds especially thiophene and thienopyridine derivatives containing the estrone nucleuous. The synthesized compounds possising antitumor and kinases inhibitions...
May 7, 2018: Anti-cancer Agents in Medicinal Chemistry
Hakimeh Mohammadi, Eszter Papp, Lindsay Cahill, Monique Rennie, Nicole Banko, Lakmini Pinnaduwage, Janice Lee, Mark Kibschull, Caroline Dunk, John G Sled, Lena Serghides
Adverse birth outcomes are common in HIV-positive pregnant women receiving combination antiretroviral therapy (cART), especially when cART is initiated in early pregnancy. The mechanisms remain poorly understood. Using a mouse model we demonstrate that protease inhibitor based-cART exposure beginning on day 1 of pregnancy was associated with a pro-angiogenic/pro-branching shift in the placenta driven by lower Flt-1 levels and higher Gcm-1 expression. Micro-CT imaging revealed an increase in the number of arterioles in cART-treated placentas, which correlated with fetal growth restriction...
April 26, 2018: Scientific Reports
B M Mendes, A V Ferreira, L T C Nascimento, S M Z M D Ferreira, M B Silveira, J B Silva
3'-Deoxy-3-[18 F]fluorothymidine, or [18 F]FLT, is a positron emission tomography (PET) tracer used in clinical studies for noninvasive assessment of proliferation activity in several types of cancer. Although the use of this PET tracer is expanding, to date, few studies concerning its dosimetry have been published. In this work, new [18 F]FLT dosimetry estimates are determined for human and mice using Monte Carlo simulations. Modern voxelized male and female phantoms and [18 F]FLT biokinetic data, both published by the ICRP, were used for simulations of human cases...
April 25, 2018: Radiation Research
S Sentürklü, D G Landblom, R Maddock, T Petry, C J Wachenheim, S I Paisley
In a 2-yr study, spring-born yearling steers (n = 144), previously grown to gain < 0.454 kg·steer-1·d-1, following weaning in the fall, were stratified by BW and randomly assigned to three retained ownership rearing systems (3-replications) in early May. Systems were: 1) feedlot (FLT), 2) steers that grazed perennial crested wheatgrass (CWG) and native range (NR) before feedlot entry (PST), and 3) steers that grazed perennial CWG and NR, and then field pea-barley mix (PBLY) and unharvested corn (UC) before feedlot entry (ANN)...
April 24, 2018: Journal of Animal Science
Yun Zhang, Yong-Gang Zhao, Wei-Sheng Chen, He-Li Cheng, Xiu-Qiong Zeng, Yan Zhu
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitously found in the environment and have been proved to be prospectively associated with the risk of cancer. In this study, a simple method based on pipette-tip solid phase extraction (PT-SPE) and gas chromatography-mass spectrometry (GC-MS) has been firstly developed for the determination of 16 PAHs in human whole blood. Three-dimensional ionic liquid-ferrite functionalized graphene oxide nanocomposite (3D-IL-Fe3 O4 -GO) was used as sorbent in PT-SPE. Compared with conventional SPE method, the PT-SPE method was solvent-saving (1...
March 21, 2018: Journal of Chromatography. A
Karin Zattar Cecyn, Eliza Y S Kimura, Dulce Marta S M Lima, Miyoko Yamamoto, José Orlando Bordin, José Salvador R de Oliveira
Background: Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors...
March 2018: Blood Research
Juliann G Kiang, Joan T Smith, Sara R Hegge, Natalia I Ossetrova
Exposure to ionizing radiation is a crucial life-threatening factor in nuclear and radiological incidents. It is known that ionizing radiation affects cytokine/chemokine concentrations in the blood of B6D2F1 mice. It is not clear whether radiation dose rates would vary the physiological response. Therefore, in this study we utilized data from two experiments using B6D2F1 female mice exposed to six different dose rates ranging from low to high rates. In one experiment, mice received a total dose of 8 Gy (LD0/30 ) of 60 Co gamma radiation at four dose rates: 0...
April 13, 2018: Radiation Research
Francesca Iommelli, Viviana De Rosa, Cristina Terlizzi, Marcello Monti, Mariarosaria Panico, Rosa Fonti, Silvana Del Vecchio
PURPOSE: Our aim was to test whether imaging with 18 F-fluorothymidine (18 F-FLT) PET/CT was able to detect the combined effects of EGFR and MET inhibitors in oncogene-driven non-small lung cancer (NSCLC) and to elucidate the mechanisms underlying the enhanced efficacy of drug combination. EXPERIMENTAL DESIGN: NSCLC cells bearing MET amplification (H1993 and H820) were treated with EGFR and MET inhibitors either alone or in combination and then tested for cell viability and inhibition of signaling...
April 4, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
R Iqbal, G M Kramer, V Frings, E F Smit, O S Hoekstra, R Boellaard
BACKGROUND: 3'-Deoxy-3'-[18 F]fluorothymidine ([18 F]FLT) was proposed as an imaging biomarker for the assessment of in vivo cellular proliferation with positron emission tomography (PET). The current study aimed to validate [18 F]FLT as a perfusion-independent PET tracer, by gaining insight in the intra-tumoural relationship between [18 F]FLT uptake and perfusion in non-small cell lung cancer (NSCLC) patients undergoing treatment with a tyrosine kinase inhibitor (TKI). Six patients with metastatic NSCLC, having an activating epidermal growth factor receptor (EGFR) mutation, were included in this study...
March 27, 2018: EJNMMI Research
Hai-Long Xu, Man Li, Rong-Jun Zhang, Hui-Jie Jiang, Ming-Yu Zhang, Xin Li, Yi-Qiao Wang, Wen-Bin Pan
BACKGROUND: Positron emission tomography (PET) is a noninvasive method to characterize different metabolic activities of tumors, providing information for staging, prognosis, and therapeutic response of patients with cancer. The aim of this study was to evaluate the feasibility of18 F-fludeoxyglucose (18 F-FDG) and 3'-deoxy-3'-18 F-fluorothymidine (18 F-FLT) PET in predicting tumor biological characteristics of colorectal cancer liver metastasis. METHODS: The uptake rate of18 F-FDG and18 F-FLT in SW480 and SW620 cells was measured via an in vitro cell uptake assay...
March 13, 2018: Hepatobiliary & Pancreatic Diseases International: HBPD INT
Hiroko Tanaka, Keiichi Kumasawa, Aiko Kakigano, Kazuya Mimura, Masayuki Endo, Takuji Tomimatsu, Tadashi Kimura
Excessive soluble fms-like tyrosine kinase-1 (sFlt-1) has been strongly implicated in preeclampsia. An increase in the serum sFlt-1 level occurs before the onset of preeclampsia, and the sFlt-1 level is already higher in women who are predisposed to preeclampsia than in normotensive pregnant women. This study aimed to investigate the relation between arginase and sFlt-1 in the plasma of preeclamptic women and normotensive pregnant women. We suggested that a regulatory mechanism exists that suppresses the level of sFlt-1...
May 5, 2018: Biochemical and Biophysical Research Communications
Nghi C Nguyen, Melissa K Yee, Abuzar M Tuchayi, John M Kirkwood, Hussein Tawbi, James M Mountz
Introduction: This pilot study aimed at exploring the utility of the proliferation tracer F-18 fluorothymidine (FLT) and positron-emission tomography (PET)/magnetic resonance imaging (MRI) (FLT-PET/MRI) for early treatment monitoring in patients with melanoma brain metastasis (MBM) who undergo targeted therapy or immunotherapy. Material and Methods: Patients with newly diagnosed MBM underwent baseline and follow-up FLT-PET/MRI scans at 3-4 weeks of targeted therapy or immunotherapy...
2018: Frontiers in Oncology
Hinrich Wieder, Ambros J Beer, Jens Siveke, Tibor Schuster, Andreas K Buck, Ken Herrmann, Jens C Stollfuss
Purpose: The aim of this study was to correlate preoperative 3'-deoxy-3'-[18 F] fluorothymidine (FLT) uptake with the clinical outcome and survival in these patients after surgery. Materials and Methods: We performed a prospective analysis in 27 patients with adenocarcinoma of the pancreas (15 males, 12 females, mean age: 62 ± 13 years, range: 34 - 86 years). FLT PET (45 min p.i., 300 MBq FLT; ECAT HR+) images were acquired according to standard protocols. FLT uptake was quantified using standardised uptake values (SUV)...
February 9, 2018: Oncotarget
Susie Choi, Hironori Uehara, Yuanyuan Wu, Subrata Das, Xiaohui Zhang, Bonnie Archer, Lara Carroll, Balamurali Krishna Ambati
Short-activating RNA (saRNA), which targets gene promoters, has been shown to increase the target gene expression. In this study, we describe the use of an saRNA (Flt a-1) to target the flt-1 promoter, leading to upregulation of the soluble isoform of Flt-1 and inhibition of angiogenesis. We demonstrate that Flt a-1 increased sFlt-1 mRNA and protein levels, while reducing VEGF expression. This was associated with suppression of human umbilical vascular endothelial cell (HUVEC) proliferation and cell cycle arrest at the G0/G1 phase...
2018: PloS One
Bianca A Trombetta, Becky C Carlyle, Aaron M Koenig, Leslie M Shaw, John Q Trojanowski, David A Wolk, Joseph J Locascio, Steven E Arnold
OBJECTIVE: Alzheimer's disease (AD) is a complex neurodegenerative disease driven by multiple interacting pathophysiological processes that ultimately results in synaptic loss, neuronal death, and dementia. We implemented a fit-for-purpose modeled approach to qualify a broad selection of commercially available immunoassays and evaluate the biotemporal stability of analytes across five pathophysiological domains of interest in AD, including core amyloid-β (Aβ) and tau AD biomarkers, neurodegeneration, inflammation/immune modulation, neurovascular injury, and metabolism/oxidative stress...
2018: PloS One
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