Yingfeng Tao, Xiaoliu Zhou, Leqiang Sun, Da Lin, Huaiyuan Cai, Xi Chen, Wei Zhou, Bing Yang, Zhe Hu, Jing Yu, Jing Zhang, Xiaoqing Yang, Fang Yang, Bang Shen, Wenbao Qi, Zhenfang Fu, Jinxia Dai, Gang Cao
In the unprecedented single-cell sequencing and spatial multiomics era of biology, fluorescence in situ hybridization (FISH) technologies with higher sensitivity and robustness, especially for detecting short RNAs and other biomolecules, are greatly desired. Here, we develop the robust multiplex π-FISH rainbow method to detect diverse biomolecules (DNA, RNA, proteins, and neurotransmitters) individually or simultaneously with high efficiency. This versatile method is successfully applied to detect gene expression in different species, from microorganisms to plants and animals...
January 27, 2023: Nature Communications