keyword
MENU ▼
Read by QxMD icon Read
search

bone marrow mesenchymal stem cell

keyword
https://www.readbyqxmd.com/read/28938612/magnetic-resonance-imaging-tracking-and-assessing-repair-function-of-the-bone-marrow-mesenchymal-stem-cells-transplantation-in-a-rat-model-of-spinal-cord-injury
#1
Hongwu Zhang, Liqin Wang, Shihong Wen, Qingfeng Xiang, Xianhong Xiang, Caixia Xu, Yong Wan, Jingnan Wang, Bin Li, Yiqian Wan, Zhiyun Yang, David Y B Deng
The transplantation of bone marrow mesenchymal stem cells (BMSCs) to repair spinal cord injury (SCI) has become a promising therapy. However, there is still a lack of visual evidence directly implicating the transplanted cells as the source of the improvement of spinal cord function. In this study, BMSCs were labeled with NF-200 promoter and lipase-activated gadolinium-containing nanoparticles (Gd-DTPA-FA). Double labeled BMSCs were implanted into spinal cord transaction injury in rat models in situ, the function recovery was evaluated on 1st, 7th, 14th, 28 th days by MRI, Diffusion Tensor Imaing, CT imaging and post-processing, and histological observations...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938423/igfbp4-is-required-for-adipogenesis-and-influences-the-distribution-of-adipose-depots
#2
David E Maridas, Victoria E DeMambro, Phuong T Le, Subburaman Mohan, Clifford J Rosen
Insulin-like Growth Factor I (IGF-I) induces adipogenesis in vitro. IGF Binding Protein 4 (IGFBP4) is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. We previously reported that Igfbp4 null mice (Igfbp4-/-) had decreased fat proportions at 8 and 16 weeks of age. However, the mechanism leading to the reduced adiposity remains unknown. The purpose of this study was to elucidate how IGFBP4 mediates adipose tissue development in vivo. Our results showed that inguinal (iWAT) and gonadal (gWAT) white adipose tissues from Igfbp4-/- mice had decreased weights and Pparγ expression...
July 27, 2017: Endocrinology
https://www.readbyqxmd.com/read/28936740/therapeutic-effects-of-mesenchymal-stem-cell-derived-exosomes-in-cardiovascular-disease
#3
Etsu Suzuki, Daishi Fujita, Masao Takahashi, Shigeyoshi Oba, Hiroaki Nishimatsu
Mesenchymal stem cells (MSCs) are multipotent stem cells that reside in various organs. They have the capacity to differentiate into various cell types, including cardiomyocytes, vascular endothelial cells, and vascular smooth muscle cells. Among the various MSCs, bone marrow-derived MSCs (BMMSCs) have been widely used for treating acute myocardial infarction (AMI) and ischemic heart failure (IHF) in preclinical and clinical studies. Although the beneficial effects of BMMSCs in treating AMI and IHF were originally attributed to their capacity to differentiate into cardiac cell types, recent evidence suggests that the differentiation capacity of BMMSCs appears to be minimal and that BMMSCs exert cardioprotective effects by secreting paracrine factors...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28934877/activin-and-nodal-are-not-suitable-alternatives-to-tgf%C3%AE-for-chondrogenic-differentiation-of-mesenchymal-stem-cells
#4
Laurie M G de Kroon, Esmeralda N Blaney Davidson, Roberto Narcisi, Eric Farrell, Peter M van der Kraan, Gerjo J V M van Osch
Objective Previously, we demonstrated the importance of transforming growth factor-β (TGFβ)-activated SMAD2/3 signaling in chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). However, TGFβ also signals via the SMAD1/5/9 pathway, which is known to induce terminal differentiation of BMSCs. In this study, we investigated whether other SMAD2/3-activating ligands, Activin and Nodal, can induce chondrogenic differentiation of BMSCs without inducing terminal differentiation. Design Activation of SMAD2/3 signaling and chondrogenesis were evaluated in human BMSCs ( N = 3 donors) stimulated with TGFβ, Activin, or Nodal...
October 2017: Cartilage
https://www.readbyqxmd.com/read/28932763/a-co-culture-assay-to-determine-efficacy-of-tnf-%C3%AE-suppression-by-biomechanically-induced-human-bone-marrow-mesenchymal-stem-cells
#5
Miguel F Diaz, Siobahn M Evans, Scott D Olson, Charles S Cox, Pamela L Wenzel
The beneficial effects of mesenchymal stem cell (MSC)-based cellular therapies are believed to be mediated primarily by the ability odansf MSCs to suppress inflammation associated with chronic or acute injury, infection, autoimmunity, and graft-versus-host disease. To specifically address the effects of frictional force caused by blood flow, or wall shear stress (WSS), on human MSC immunomodulatory function, we have utilized microfluidics to model WSS at the luminal wall of arteries. Anti-inflammatory potency of MSCs was subsequently quantified via measurement of TNF-α production by activated murine splenocytes in co-culture assays...
August 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28931727/co-culture-of-allogenic-dbm-and-bmscs-in-the-knee-joint-cavity-of-rabbits-for-cartilage-tissue-engineering
#6
Bin Xu, Rui Wang, Hao Wang, Hong-Gang Xu
This study aims to assess co-culture of allogenic decalcified bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) in the knee joint cavity of rabbits for cartilage tissue engineering. Rabbits were assigned to an in-vitro group, an in-vivo group and a blank control group. At the 4(th), 8(th) and 12(th) week, samples from all groups were collected for hematoxylin-eosin (HE) staining and streptavidin-perosidase (SP) method. The morphological analysis software was used to calculate the average absorbance value (A value)...
September 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28929517/neural-differentiation-of-bone-marrow-mesenchymal-stem-cells-with-human-brain-derived-neurotrophic-factor-gene-modified-in-functionalized-self-assembling-peptide-hydrogel-in-vitro
#7
Hongbin Luo, Changsheng Xu, Zhiwei Liu, Lin Yang, Yunda Hong, Guisheng Liu, Huifen Zhong, Xinyi Cai, Xuping Lin, Xiaokun Chen, Changsheng Wang, Nanwen Zhang, Weihong Xu
OBJECTIVE: To investigate the biocompatibility and differentiation of human brain-derived neurotrophic factor (hBDNF) gene-modified bone marrow mesenchymal stem cells (hBDNF-rMSCs) in a functionalized self-assembling peptide hydrogel. METHODS: hBDNF were engineered in rMSCs using adenovirus vector and the enhanced green fluorescence protein (eGFP) was used as a reporter gene. MSC-specific surface markers (CD90,CD29 and CD45)were used for identifying rMSCs. Fluorescence microscope was used to detect the transfection of rMSCs...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28928910/phase-i-ii-randomized-controlled-trial-of-autologous-bone-marrow-derived-mesenchymal-stem-cell-therapy-for-chronic-stroke
#8
Kam Sze Tsang, Chi Ping Stephanie Ng, Xian Lun Zhu, George Kwok Chu Wong, Gang Lu, Anil Tejbhan Ahuja, Ka Sing Lawrence Wong, Ho Keung Ng, Wai Sang Poon
AIM: To examine the safety and efficacy of mesenchymal stem cell (MSC) therapy for intracerebral haemorrhage with neurological dysfunctions for a year. METHODS: MSC were ex vivo expanded from 29 mL (17-42 mL) autologous bone marrow. Patients were randomized to have two intravenous injections of autologous MSC or placebos in four weeks apart. Neurological functions and clinical outcomes were monitored before treatment and at 12(th), 16(th), 24(th), 36(th) and 60(th) week upon completion of the treatment...
August 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/28928773/mesenchymal-stem-cells-in-restoration-of-fertility-at-experimental-pelvic-inflammatory-disease
#9
Nataliia Volkova, Mariia Yukhta, Anatoliy Goltsev
Inflammatory disorders account for a significant percentage of gynecologic diseases, particularly in women of reproductive age. It is known that stem cells have anti-inflammatory and regenerative properties. Based on this, we investigated the effect of intravenous administration of cryopreserved mesenchymal stem cells (cMSCs) of bone marrow on experimental chronic inflammation of the ovaries. The paper shows that on the 21st day after cMSC therapy, leukocyte infiltration of ovaries was slightly relative to the control group without treatment, and the ratio of developing and atretic follicles in the animals with cMSC injection dramatically increased, while in the control, it still remained on the side of atretic forms...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28927682/mgf-e-peptide-pretreatment-improves-the-proliferation-and-osteogenic-differentiation-of-bmscs-via-mek-erk1-2-and-pi3k-akt-pathway-under-severe-hypoxia
#10
Yongqiang Sha, Yonggang Lv, Zhiling Xu, Li Yang, Xiaoying Hao, Ruli Afandi
AIMS: Severe hypoxia always inhibits the cell proliferation, osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs), and hinders bone defect repair. Herein we explored the effects of mechano-growth factor (MGF) E peptide on the proliferation and osteogenic differentiation of BMSCs under severe hypoxia. MATERIALS AND METHODS: CoCl2 was utilized to simulate severe hypoxia. MTS was used to detect cell viability. Cell proliferation was verified through flow cytometry and EdU assay...
September 16, 2017: Life Sciences
https://www.readbyqxmd.com/read/28926112/chronic-kidney-disease-and-vitamin-d-metabolism-in-human-bone-marrow-derived-mscs
#11
REVIEW
Shuanhu Zhou, Julie Glowacki
Vitamin D that is synthesized in the skin or is ingested undergoes sequential steps of metabolic activation via a cascade of cytochrome P450 enzymatic hydroxylations in the liver and kidney to produce 1α,25-dihydroxyvitamin D (1α,25(OH)2 D). There are many tissues that are able to synthesize 1α,25(OH)2 D, but the biological significance of extrarenal hydroxylases is unresolved. Human marrow-derived mesenchymal stem cells (marrow stromal cells, hMSCs) give rise to osteoblasts, and their differentiation is stimulated by 1α,25(OH)2 D...
August 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28926092/key-genes-regulating-the-liposecretion-process-of-mature-adipocytes
#12
Giulia Maurizi, Tiina Petäistö, Angela Maurizi, Lucio Della Guardia
White mature adipocytes (MAs) are plastic cells able to reversibly transdifferentiate toward fibroblast-like cells maintaining stem cell gene signatures. The main morphologic aspect of this transdifferentiation process, called liposecretion, is the secretion of large lipid droplets and the development of organelles necessary for exocrine secretion. There is a considerable interest in the adipocyte plastic properties involving liposecretion process, but the molecular details are incompletely explored. This review analyzes the gene expression of MAs isolated from human subcutaneous fat tissue with respect to bone marrow (BM)-derived mesenchymal stem cells (MSC) focusing on gene regulatory pathways involved into cellular morphology changes, cellular proliferation and transports of molecules through the membrane, suggesting potential ways to guide liposecretion...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28926091/functional-role-of-mesenchymal-stem-cells-in-the-treatment-of-chronic-neurodegenerative-diseases
#13
Debora Lo Furno, Giuliana Mannino, Rosario Giuffrida
Mesenchymal stem cells (MSCs) can differentiate into not only cells of mesodermal lineages, but also into endodermal and ectodermal derived elements, including neurons and glial cells. For this reason, MSCs have been extensively investigated to develop cell-based therapeutic strategies, especially in pathologies whose pharmacological treatments give poor results, if any. As in the case of irreversible neurological disorders characterized by progressive neuronal death, in which behavioral and cognitive functions of patients inexorably decline as the disease progresses...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28925497/the-feasibility-of-the-cd271-and-cd271-mesenchymal-stromal-cell-enrichment-toward-nucleus-pulposus-like-cells
#14
Katarzyna Jezierska-Wozniak, Monika Barczewska, Aleksandra Habich, Pawel Wojtacha, Wanda Badowska, Wojciech Maksymowicz, Joanna Wojtkiewicz
INTRODUCTION: Factors promoting nerve cell ingrowth are considered responsible for chronic back pain resulting from the intervertebral disc degeneration (IDD). One of the recent exploratory IDD treatments is stem cell transplantation therapy. The CD271 (low-affinity nerve growth factor receptor) has been identified as a marker of the most homogeneous mesenchymal stem cell (MSC) subset. It is capable of promoting differentiation along adipogenic, osteogenic and chondrogenic lineages and producing significantly higher levels of cytokines as compared to the total population of plastic adherence-mesenchymal stem cells (PA-MSCs)...
September 19, 2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28923793/dose-comparison-study-of-allogeneic-mesenchymal-stem-cells-in-patients-with-ischemic-cardiomyopathy-the-trident-study
#15
Victoria Florea, Angela C Rieger, Darcy L Difede, Jill El-Khorazaty, Makoto Natsumeda, Monisha N Banerjee, Bryon A Tompkins, Aisha Khan, Ivonne H Schulman, Ana M Landin, Muzammil Mushtaq, Maureen H Lowery, John Byrnes, Robert C Hendel, Mauricio G Cohen, Krystalenia Valasaki, Marietsy V Pujol, Eduard Ghersin, Roberto Miki, Cindy Delgado, Fouad A Abuzeid, Mayra Vidro-Casiano, Russell Saltzman, Daniel DaFonseca, Lina V Caceres, Kevin N Ramdas, Adam Mendizabal, Alan W Heldman, Raul D Mitrani, Joshua M Hare
Rationale: Cell dose and concentration play crucial roles in phenotypic responses to cell-based therapy for heart failure. Objective: To compare the safety and efficacy of two doses of allogeneic bone marrow-derived human mesenchymal stem cells (hMSC) identically delivered in patients with ischemic cardiomyopathy (ICM). Methods and Results: Thirty patients with ICM received in a blinded manner either 20 million (20M, n=15) or 100 million (100M, n=15) allogeneic hMSCs via transendocardial injection (10 0.5 cc injections/patient)...
September 18, 2017: Circulation Research
https://www.readbyqxmd.com/read/28923246/klf15-regulates-in-vitro-chondrogenic-differentiation-of-human-mesenchymal-stem-cells-by-targeting-sox9
#16
Zhuoyue Song, Xiaolei Lian, Yang Wang, Yong Xiang, Guangheng Li
Mesenchymal stem cells (MSCs) are multipotent stromal cells residing in the bone marrow. MSCs have the potential to differentiate into adipocytes, chondrocytes, and other types of cells. However, the mechanism underlying MSC differentiation is still not fully understood. Here we aimed to investigate the function of the Kruppel-like factor (KLF) transcriptional factor family in regulating chondrogenic differentiation from human MSCs. Among the KLF family members, KLF15 was activated during different models of chondrogenic differentiation in a time-dependent manner...
September 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28921810/bones-morphogenic-protein-4-and-retinoic-acid-combined-treatment-comparative-analysis-for-in-vitro-differentiation-potential-of-murine-mesenchymal-stem-cells-derived-from-bone-marrow-and-adipose-tissue-into-germ-cells
#17
Maryam H Shirzeily, Neda Khanlarkhani, Fardin Amidi, Farshad H Shirzeily, Fereydoon S Aval, Aligholi Sobhani
Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to investigate the efficacy of a combined medium, retinoic acid (RA) together with Bone Morphogenic Protein-4 (BMP4), on differentiation of Bone Marrow Mesenchymal Stem Cells (BMMSCs) and adipose-derived mesenchymal stem cells (ADMSCs) into germ cells...
September 18, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28920397/-effects-of-interleukin-34-expressed-by-human-bone-marrow-derived-mesenchymal-stem-cells-on-thp-1-cells
#18
Xueming Gao, Shun Zhang, Zhaojun Xu, Yan Shan, Zihong Wang, Ting Cai, Xue Jiang
To construct recombinant eukaryotic expression plasmid vector of human IL-34 gene, and to study the effects of IL-34 expressed by human bone marrow-derived mesenchymal stem cells (hBM-MSCs) on THP-1 cells. Full-length IL-34 encoding sequence was amplified by PCR. And this fragment was cloned into the plasmid pIRES2-EGFP. Western blotting and ELISA were used to analyze the expression of IL-34 in hBM-MSCs. THP-1 cells were cultured with hBM-MSCs medium containing IL-34 protein. Real-time PCR detected the effects of IL-34 on the expression of IL-10 and TNFα in THP-1 cells...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28920062/into-the-eyes-of-bone-marrow-derived-mesenchymal-stem-cells-therapy-for-myocardial-infarction-and-other-diseases
#19
REVIEW
Jian-Rui Li, Ting-Ting Qu
Applications of bone marrow-derived mesenchymal stem cells (BM-MSCs) have been documented for diseases occur in the sports system, the central nervous system, the cardiovascular system etc. However, poor viability of donor stem cells after transplantation limits their therapeutic efficiency. Although the autophagy theory has been reported, the underlying mechanisms are still poorly understood. Isolation and culture methods of mesenchymal stem cells are currently concentrate on four ways. Overall, BM-MSCs have both important research significance and clinical application value in cell replacement therapy, gene therapy and reconstruction of tissues as well as organs especially for myocardial infarction (MI)...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/28919892/serum-mediated-oxidative-stress-from-systemic-sclerosis-patients-affects-mesenchymal-stem-cell-function
#20
Guillaume Fonteneau, Claire Bony, Radjiv Goulabchand, Alexandre T J Maria, Alain Le Quellec, Sophie Rivière, Christian Jorgensen, Philippe Guilpain, Danièle Noël
OBJECTIVES: Properties of mesenchymal stromal/stem cells (MSCs) from systemic sclerosis (SSc) patients have been reported to be altered. MSC-based therapy may therefore rely on the use of allogeneic MSCs from healthy subjects. Here, we investigated whether heterologous MSCs could exhibit altered properties following exposure to oxidative environment of SSc sera. METHODS: Human bone marrow-derived MSCs were cultured in the presence of various sera: control human serum AB (SAB), SAB with HOCl-induced AOPPs at 400 or 1,000 µmol/L (SAB400 or SAB1000, respectively), or H2O2-induced AOPPs or SSc patient serum (PS)...
2017: Frontiers in Immunology
keyword
keyword
39708
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"