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bone marrow mesenchymal stem cell

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https://www.readbyqxmd.com/read/29150954/physiological-role-of-urothelial-cancer-associated-1-long-noncoding-rna-in-human-skeletogenic-cell-differentiation
#1
Takanori Ishikawa, Takashi Nishida, Mitsuaki Ono, Takeshi Takarada, Nguyen Ha, Shinnosuke Kurihara, Takayuki Furumatsu, Yurika Murase, Masaharu Takigawa, Toshitaka Oohashi, Hiroshi Kamioka, Satoshi Kubota
A vast number of long-noncoding RNAs (lncRNA) are found expressed in human cells, which RNAs have been developed along with human evolution. However, the physiological functions of these lncRNAs remain mostly unknown. In the present study, we for the first time uncovered the fact that one of such lncRNAs plays a significant role in the differentiation of chondrocytes and, possibly, of osteoblasts differentiated from mesenchymal stem cells, which cells eventually construct the human skeleton. The urothelial cancer-associated 1 (UCA1) lncRNA is known to be associated with several human malignancies...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29150876/toxicological-characterization-of-zno-nanoparticles-in-malignant-and-non-malignant-cells
#2
Helena Moratin, Agmal Scherzad, Thomas Gehrke, Pascal Ickrath, Katrin Radeloff, Norbert Kleinsasser, Stephan Hackenberg
The increasing usage of zinc oxide nanoparticles (ZnO-NPs) in industrial applications as well as in consumer products raises concern regarding their potential adverse effects to a greater extend. Numerous studies have demonstrated toxic properties of NPs, however there is still a lack of knowledge concerning the underlying mechanisms. This study was designed to systematically investigate cytotoxicity, apoptosis, cell cycle alterations, and genotoxicity induced by ZnO-NP. Moreover, it was an aim of the investigations to specify the diverse effects of nanoparticle exposure in malignant in comparison with non-malignant cells...
November 18, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29149982/marrow-mesenchymal-stem-cells-effectively-reduce-histologic-changes-in-a-rat-model-of-chronic-renal-allograft-rejection
#3
P Yu, Z Wang, Y Liu, Z Xiao, Y Guo, M Li, M Zhao
BACKGROUND: Chronic allograft rejection remains as the leading cause of the chronic renal grafts loss post-transplantation. No therapy has been found really effective to prevent and treat chronic allograft rejection. Mesenchymal stem cells (MSCs) have characteristics of immunomodulation and are expected to be used for inducing graft immune tolerance in organ transplantation. We investigated the efficacy and safety of early infusion of donor-derived marrow MSCs in a rat model of chronic renal allograft rejection...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29146005/bone-marrow-adipocytes-in-haematological-malignancies
#4
Ewa Frączak, Mateusz Olbromski, Aleksandra Piotrowska, Natalia Glatzel-Plucińska, Piotr Dzięgiel, Jarosław Dybko, Kazimierz Kuliczkowski, Tomasz Wróbel
Bone marrow adipocytes (BMAs) derived from mesenchymal stem cells (MSC) are an active and significant element of the bone marrow microenvironment. They are involved in metabolic functions, complex interactions with other stromal cells, and in the development and progression of tumours. Currently, there is little data regarding the role of BMAs in haematological malignancies. Due to this, we have attempted to characterise the BMAs in these malignancies in terms of quantity and morphology. Our study included 30 patients aged 22-76 with myelo- (n=17) and lymphoproliferative malignancies (n=13), both with and without bone marrow infiltration...
November 13, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29145157/bmscs-derived-mir-223-containing-exosomes-contribute-to-liver-protection-in-experimental-autoimmune-hepatitis
#5
Lu Chen, Feng-Bin Lu, Da-Zhi Chen, Jin-Lu Wu, En-de Hu, Lan-Man Xu, Ming-Hua Zheng, Hui Li, Yu Huang, Xiao-Ya Jin, Yue-Wen Gong, Zhuo Lin, Xiao-Dong Wang, Yong-Ping Chen
Autoimmune hepatitis is a chronic inflammatory disease in the liver with potential to the development of liver fibrosis. Recent evidences suggest that bone marrow derived mesenchymal stem cells (BMSCs) may exert its therapeutic activity through exosomes. Moreover, miR-223 is highly expressed in BMSCs and plays an important role in autoimmune diseases. Therefore, in this study, hepatoprotective role of BMSCs and miR-223 was investigated in both mice and hepatocytes. Liver antigen S100 was used to establish autoimmune hepatitis model in mice while LPS and ATP were used to establish cell injury model in hepatocyte...
November 13, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29144199/effect-of-high-hydrostatic-pressure-applied-before-cryopreservation-on-the-survival-rate-and-quality-of-porcine-mesenchymal-stem-cells-after-thawing
#6
Joanna Romanek, Jolanta Opiela, Daniel Lipiński, Zdzisław Smorąg
The aim of the present study was to examine the effects of varied high hydrostatic pressure (HHP) values on survival rate, proliferation rate, cell multipotency (transcript expression of SOX2, C-MYC, and REX1) and apoptosis (expression of phosphatidylserine (PS), SURVIVIN at the RNA level and BAX at the protein level) of porcine mesenchymal stem cells (MSCs). MSCs were isolated from porcine bone marrow and cultured in vitro. Before cryopreservation and storage in liquid nitrogen, MSCs were subjected to HHP at the varied pressures of 20, 30, 40, 50, or 60 MPa for 1 h at 24°C...
November 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29142785/role-of-cord-blood-and-bone-marrow-mesenchymal-stem-cells-in-recent-deep-burn-a-case-control-prospective-study
#7
Wael Abo-Elkheir, Fawzy Hamza, Ahmed M Elmofty, Atef Emam, Magdy Abdl-Moktader, Sameh Elsherefy, Hala Gabr
RATIONALE: Burn injuries represent one of the major worldwide public health problems causing more severe physiological stress than other traumas. Effective treatment of burn injuries is mandatory to prevent the numerous life-threatening complications and possible disabilities. Stem cells, a population of multipotent cells retaining the properties of self-renewal and differentiation, are the main player in tissue regeneration after major trauma. Thus, they are thought to play a key role in wound healing inducing efficient and physiological skin regeneration...
2017: American Journal of Stem Cells
https://www.readbyqxmd.com/read/29141554/insight-into-the-role-of-long-non-coding-rnas-during-osteogenesis-in-mesenchymal-stem-cells
#8
Sibei Huo, Yachuan Zhou, Xinyu He, Mian Wan, Wei Du, Xin Xu, Ling Ye, Xuedong Zhou, Liwei Zheng
BACKGROUND: Long non-coding RNAs (LncRNAs) are non-protein coding transcripts longer than 200 nucleotides in length. Instead of being "transcriptional noise", lncRNAs are emerging as a key modulator in various biological processes and disease development. Mesenchymal stem cells can be isolated from various adult tissues, such as bone marrow and dental tissues. The differentiation processes into multiple lineages, such as osteogenic differentiation, are precisely orchestrated by molecular signals in both genetic and epigenetic ways...
November 15, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29141446/myocardial-reparative-functions-of-exosomes-from-mesenchymal-stem-cells-are-enhanced-by-hypoxia-treatment-of-the-cells-via-transferring-microrna-210-in-an-nsmase2-dependent-way
#9
Jinyun Zhu, Kai Lu, Ning Zhang, Yun Zhao, Qunchao Ma, Jian Shen, Yinuo Lin, Pingping Xiang, Yaoliang Tang, Xinyang Hu, Jinghai Chen, Wei Zhu, Keith A Webster, Jian'an Wang, Hong Yu
Hypoxia treatment enhances paracrine effect of mesenchymal stem cells (MSCs). The aim of this study was to investigate whether exosomes from hypoxia-treated MSCs (Exo(H)) are superior to those from normoxia-treated MSCs (Exo(N)) for myocardial repair. Mouse bone marrow-derived MSCs were cultured under hypoxia or normoxia for 24 h, and exosomes from conditioned media were intramyocardially injected into infarcted heart of C57BL/6 mouse. Exo(H) resulted in significantly higher survival, smaller scar size and better cardiac functions recovery...
November 16, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29140298/enhancement-of-bone-marrow-derived-mesenchymal-stem-cell-osteogenesis-and-new-bone-formation-in-rats-by-obtusilactone-a
#10
Yi-Hsiung Lin, Chung-Yi Chen, Liang-Yin Chou, Chung-Hwan Chen, Lin Kang, Chau-Zen Wang
The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis markers BMP2, Runx2, Collagen I, and Osteocalcin was enhanced in OA-treated BMSCs. An in vivo rat model with local administration of OA via needle implantation to bone marrow-residing BMSCs revealed that OA increased the new bone formation and trabecular bone volume in tibias...
November 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29139088/contrasting-views-on-the-role-of-mesenchymal-stromal-stem-cells-in-tumour-growth-a-systematic-review-of-experimental-design
#11
Ahmed Kolade Oloyo, Melvin Anyasi Ambele, Michael Sean Pepper
The effect of mesenchymal stromal/stem cells (MSCs) on tumour growth remains controversial. Experimental evidence supports both an inhibitory and a stimulatory effect. We have assessed factors responsible for the contrasting effects of MSCs on tumour growth by doing a meta-analysis of existing literature between 2000 and May 2017. We assessed 183 original research articles comprising 338 experiments. We considered (a) in vivo and in vitro experiments, (b) whether in vivo studies were syngeneic or xenogeneic, and (c) if animals were immune competent or deficient...
November 15, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29138837/vegf-secreted-by-mesenchymal-stem-cells-mediates-the-differentiation-of-endothelial-progenitor-cells-into-endothelial-cells-via-paracrine-mechanisms
#12
Quanhu Ge, Hongwei Zhang, Jixue Hou, Longfei Wan, Wenzhe Cheng, Xiaoyi Wang, Dan Dong, Congzhe Chen, Jie Xia, Jun Guo, Xueling Chen, Xiangwei Wu
Stem cell therapy is a promising treatment strategy for ischemic diseases. Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) adhere to each other in the bone marrow cavity and in in vitro cultures. We have previously demonstrated that the adhesion between MSCs and EPCs is critical for MSC self‑renewal and their multi‑differentiation into osteoblasts and chondrocytes. In the present study, the influence of the indirect communication between EPCs and MSCs on the endothelial differentiation potential of EPCs was investigated, and the molecular mechanisms underlying MSC‑mediated EPC differentiation were explored...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138639/involvement-of-wnt-signaling-in-the-regulation-of-gestational-age-dependent-umbilical-cord-derived-mesenchymal-stem-cell-proliferation
#13
Sota Iwatani, Akemi Shono, Makiko Yoshida, Keiji Yamana, Khin Kyae Mon Thwin, Jumpei Kuroda, Daisuke Kurokawa, Tsubasa Koda, Kosuke Nishida, Toshihiko Ikuta, Kazumichi Fujioka, Masami Mizobuchi, Mariko Taniguchi-Ikeda, Ichiro Morioka, Kazumoto Iijima, Noriyuki Nishimura
Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29138545/activation-of-notch1-signaling-alleviates-dysfunction-of-bone-marrow-derived-mesenchymal-stem-cells-induced-by-cigarette-smoke-extract
#14
Yi Cheng, Wen Gu, Guorui Zhang, Xiaoming Li, Xuejun Guo
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are considered attractive therapeutic agents for the treatment of COPD. However, little is known about the impact of Notch on the proliferation, migration, and survival of MSCs in a cigarette smoke (CS) microenvironment. Here, we used CS extract to mimic the CS microenvironment in vitro, with the intention to investigate the effect of Notch in regulating proliferation, migration, and survival of BM-MSCs. Rat bone marrow mesenchymal stem cells were infected with lentivirus vector containing the intracellular domain of Notch1 (N1ICD) and challenged with CS extract...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/29138357/tadalafil-a-long-acting-phosphodiesterase-inhibitor-promotes-bone-marrow-stem-cell-survival-and-their-homing-into-ischemic-myocardium-for-cardiac-repair
#15
Ibrahim Elmadbouh, Muhammad Ashraf
The aim was to evaluate the tadalafil-mediated effects at molecular level on bone marrow-derived mesenchymal stem cells (MSCs) survival and their homing into the infarcted hearts to promote cardiac repair and improve function. MSCs were pretreated in vitro with inhibitors of PKG, MAPK, FasL, nitric oxide synthase (NOS) (L-NAME), CXCR4 (AMD3100), or miR-21 inhibitors (+/-luciferase construction +/-Fas) prior to tadalafil treatment for 2 h. These MSCs were then subjected to H2O2 stress to assess their injury...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29138221/immature-cml-cells-implement-a-bmp-autocrine-loop-to-escape-tki-treatment
#16
Elodie Grockowiak, Bastien Laperrousaz, Sandrine Jeanpierre, Thibault Voeltzel, Boris Guyot, Stéphanie Gobert, Franck E Nicolini, Véronique Maguer-Satta
The BCR-ABL specific Tyrosine Kinase Inhibitors (TKI) changed the outcome of Chronic Myeloid Leukemia (CML), turning a life-threatening disease into a chronic illness. However, TKI are not yet curative, since most patients retain leukemic stem cells (LSC) and their progenitors in bone marrow and relapse following treatment cessation. At diagnosis, deregulations of the Bone Morphogenetic Proteins (BMP) pathway are involved in LSC and progenitors expansion. Here, we report that BMP pathway alterations persist in TKI-resistant patients...
November 14, 2017: Blood
https://www.readbyqxmd.com/read/29137914/orthogonal-potency-analysis-of-mesenchymal-stromal-cell-function-during-ex-vivo-expansion
#17
Danika Khong, Matthew Li, Amy Singleton, Ling-Yee Chin, Shilpaa Munkundan, Biju Parekkadan
Adult bone marrow mesenchymal stromal cells (MSCs) have cross-functional, intrinsic potency that is of therapeutic interest. Their ability to regenerate bone, fat, and cartilage, modulate the immune system, and nurture the growth and function of other bone marrow hematopoietic stem/progenitor cells have all been evaluated by transplant applications of MSCs. These applications require the isolation and expansion scaled cell production. To investigate biophysical properties of MSCs that can be feasibly utilized as predictors of bioactivity during biomanufacturing, we used a low-density seeding model to drive MSCs into proliferative stress and exhibit the hallmark characteristics of in vitro aging...
November 11, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29137353/mesenchymal-stem-cells-inhibit-rank-rankl-interactions-between-osteoclasts-and-th17-cells-via-osteoprotegerin-activity
#18
Kyung-Ah Cho, Minhwa Park, Yu-Hee Kim, Kyung-Ha Ryu, So-Youn Woo
Th17 cells play a critical role in several autoimmune diseases, including psoriasis and psoriatic arthritis (PsA). Psoriasis is a chronic inflammatory skin disease associated with systemic inflammation and comorbidities, such as PsA. PsA develops in nearly 70% of patients with psoriasis, and osteoclasts associated bone erosion is a hallmark of the disease. Thus far, the effect of Th17 cells on osteoclastogenesis via direct cell-to-cell interactions is less understood. In this study, we observed that Th17 cells directly promote osteoclast differentiation and maturation via expression of receptor activator of nuclear factor-κ β ligand (RANKL) in vitro...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29134302/nuclear-organization-during-in-vitro-differentiation-of-porcine-mesenchymal-stem-cells-mscs-into-adipocytes
#19
Joanna Stachecka, Agnieszka Walczak, Beata Kociucka, Błażej Ruszczycki, Grzegorz Wilczyński, Izabela Szczerbal
Differentiation of progenitor cells into adipocytes is accompanied by remarkable changes in cell morphology, cytoskeletal organization, and gene expression profile. Mature adipocytes are filled with a large lipid droplet and the nucleus tends to move to the cell periphery. It was hypothesized that the differentiation process is also associated with changes of nuclear organization. The aim of this study was to determine the number and distribution of selected components of nuclear architecture during porcine in vitro adipogenesis...
November 13, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29133959/a-novel-cytotherapy-device-for-rapid-screening-enriching-and-combining-mesenchymal-stem-cells-into-a-biomaterial-for-promoting-bone-regeneration
#20
Yifu Zhuang, Yaokai Gan, Dingwei Shi, Jie Zhao, Tingting Tang, Kerong Dai
Bone defects are a common challenge in clinic, usually warranting bone grafts. However, current strategies to obtain effective graft materials have many drawbacks. Mesenchymal stem cell (MSC)-based therapy is a promising alternative. We designed an innovative appliance named the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS). In this study, 42 patients who required bone graft underwent SECCS-based treatment. Their bone marrow samples and beta-tricalcium phosphate (β-TCP) granules were processed in the SECCS for 10-15 minutes, to produce MSC/β-TCP composites...
November 13, 2017: Scientific Reports
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