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bone marrow mesenchymal stem cell

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https://www.readbyqxmd.com/read/28748482/morphological-analysis-of-biocompatibility-of-autologous-bone-marrow-mononuclear-cells-with-synthetic-polyethylene-terephthalate-scaffold
#1
I V Gilevich, I S Polyakov, V A Porkhanov, V P Chekhonin
We studied the properties of a tissue-engineered trachea consisting of a polyethylene terephthalate scaffold populated with autologous bone marrow mononuclear cells. The tissue-engineered constructs were obtained before surgery, during the postoperative period, and during autopsy. Cytomorphological analysis during the postoperative period showed the presence of mesenchymal stem cells on the inner surface of the implant on day 3 after surgery and cells of the respiratory epithelium on day 10-14. In autopsy samples, single epithelial cells, endothelial cells, and basal cells were found...
July 26, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28746817/human-bone-marrow-mesenchymal-stem-cells-bmscs-suppress-hepatic-stellate-cells-hscs-proliferation-by-inhibiting-the-ubiquitylation-of-p27
#2
Liang Wang, Guang Bai, Fei Chen
Bone marrow mesenchymal stem cells (BMSCs) have considerable therapeutic potential for the treatment of end-stage liver disease. Previous studies have demonstrated that BMSCs secreted growth factors and cytokines to inactivate hepatic stellate cells (HSCs), which inhibited the progression of hepatic fibrosis. The aim of this study was to determine the mechanism by which BMSCs suppress the function of HSCs in fibrosis. Our results showed that co-culture of BMSCs and HSCs induced cell cycle arrest at the G10/G1 phase and cell apoptosis of HSCs, which finally inhibited the cell proliferation of HSCs...
July 26, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28743269/mesenchymal-stem-cells-from-sternum-the-type-of-heart-disease-ischemic-or-valvular-does-not-influence-the-cell-culture-establishment-and-growth-kinetics
#3
Lucinara Dadda Dias, Karina Rabello Casali, Carine Ghem, Melissa Kristocheck da Silva, Grasiele Sausen, Patrícia Bonini Palma, Dimas Tadeu Covas, Renato A K Kalil, Beatriz D Schaan, Nance Beyer Nardi, Melissa Medeiros Markoski
BACKGROUND: In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results. METHODS: To study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases...
July 25, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28740146/in-vivo-imaging-to-monitor-differentiation-and-therapeutic-effects-of-transplanted-mesenchymal-stem-cells-in-myocardial-infarction
#4
Zhijun Pei, Jing Zeng, Yafeng Song, Yan Gao, Ruimin Wu, Yijia Chen, Fuyan Li, Wei Li, Hong Zhou, Yi Yang
Here, we used a noninvasive multimodality imaging approach to monitor differentiation of transplanted bone marrow mesenchymal stem cells (BMSCs) and recovery of cardiac function in an in vivo model of myocardial infarction (MI). We established a rat MI model by coronary artery ligation. Ninety rats were randomly assigned into four groups: sham-operated, MI model, and α-MHC-HSV1-tk-transfected or un-transfected BMSCs-treated MI model. We used (18)F-Fluro-deoxyglucose ((18)F-FDG) positron emission tomography (PET) to monitor recovery of cardiac function, and (18)F-FHBG PET/CT imaging to monitor transplanted BMSCs differentiation 24 h after (18)F-FDG imaging...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28739828/mice-bone-marrow-sca-1-cd44-mesenchymal-stem-cells-kill-avirulent-mycobacteria-but-not-mycobacterium-tuberculosis-through-modulation-of-cathelicidin-expression-via-p38-mapk-dependent-pathway
#5
Sumanta Kumar Naik, Avinash Padhi, Geetanjali Ganguli, Srabasti Sengupta, Sanghamitra Pati, Dasarathi Das, Avinash Sonawane
Mycobacterium tuberculosis (Mtb) primarily infects lung macrophages. However, recent study has shown that Mtb also infect and persist in dormant form inside the bone marrow mesenchymal stem cells (BM-MSCs) even after successful antibiotic therapy. However, the mechanism(s) by which Mtb survives in BM-MSCs is still not known. Like macrophages, BM-MSCs do not contain a well-defined endocytic pathway, which is known to play a central role in the clearance of internalized mycobacteria. Here, we studied the fate of virulent and avirulent mycobacteria in Sca-1(+)/CD44(+) BM-MSCs...
July 24, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28739546/in-situ-tissue-regeneration-through-sdf-1%C3%AE-driven-cell-recruitment-and-stiffness-mediated-bone-regeneration-in-a-critical-sized-segmental-femoral-defect
#6
Amaia Cipitria, Kathrin Boettcher, Sophia Schoenhals, Daniela S Garske, Katharina Schmidt-Bleek, Agnes Ellinghaus, Anke Dienelt, Anja Peters, Manav Mehta, Christopher M Madl, Nathaniel Huebsch, David J Mooney, Georg N Duda
In-situ tissue regeneration aims to utilize the body's endogenous healing capacity through the recruitment of host stem or progenitor cells to an injury site. Stromal cell-derived factor-1 α (SDF-1 α) is widely discussed as a potent chemoattractant. Here we use a cell-free biomaterial-based approach to (i) deliver SDF-1 α for the recruitment of endogenous bone marrow-derived stromal cells (BMSC) into a critical-sized segmental femoral defect in rats and to (ii) induce hydrogel stiffness-mediated osteogenic differentiation in-vivo...
July 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28739509/proteomic-analysis-of-the-secretome-of-human-bone-marrow-derived-mesenchymal-stem-cells-primed-by-pro-inflammatory-cytokines
#7
Elisa Maffioli, Nonnis Simona, Roberta Angioni, Fabiana Santagata, Bianca Calì, Lucia Zanotti, Armando Negri, Antonella Viola, Gabriella Tedeschi
Mesenchymal stem cells (MSC) represent an impressive opportunity in term of regenerative medicine and immunosuppressive therapy. Although it is clear that upon transplantation MSC exert most of their therapeutic effects through the secretion of bioactive molecules, the effects of a pro-inflammatory recipient environment on MSC secretome have not been characterized. In this study, we used a label free mass spectrometry based quantitative proteomic approach to analyze how pro-inflammatory cytokines modulate the composition of the human MSC secretome...
July 21, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28738525/mesenchymal-stem-cells-with-igf-1-and-tgf-%C3%AE-1-in-laminin-gel-for-osteochondral-defects-in-rabbits
#8
Mudasir Bashir Gugjoo, Amarpal, Ahmed Abdelbaset-Ismail, Hari Prasad Aithal, Prakash Kinjavdekar, Abhijit Motiram Pawde, Gutulla Sai Kumar, Gutulla Taru Sharma
OBJECTIVE: Healing of articular cartilage is still a challenge due to its limited potential to regenerate. In the present study, we evaluated allogenic bone marrow mesenchymal stem cells (BM-MSCs) alone or in combination with growth factors, insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) in laminin scaffolds for healing of osteochondral defects. DESIGN: Osteochondral defects of 4mm (diameter) x 5mm (depth) were induced in the rabbit knee joints and treated with phosphate-buffered saline (PBS; control), BM-MSCs, BM-MSCs in laminin, BM-MSCs in laminin with IGF-1, or BM-MSCs in laminin with IGF-1 and TGF-β1 in 10 animals each...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28736687/tissue-engineered-tendon-for-enthesis-regeneration-in-a-rat-rotator-cuff-model
#9
Michael J Smietana, Pablo Moncada-Larrotiz, Ellen M Arruda, Asheesh Bedi, Lisa M Larkin
Healing of rotator cuff (RC) injuries with current suture or augmented scaffold techniques fails to regenerate the enthesis and instead forms a weaker fibrovascular scar that is prone to subsequent failure. Regeneration of the enthesis is the key to improving clinical outcomes for RC injuries. We hypothesized that the utilization of our tissue-engineered tendon to repair either an acute or a chronic full-thickness supraspinatus tear would regenerate a functional enthesis and return the biomechanics of the tendon back to that found in native tissue...
2017: BioResearch Open Access
https://www.readbyqxmd.com/read/28734835/migration-ability-and-toll-like-receptor-expression-of-human-mesenchymal-stem-cells-improves-significantly-after-three-dimensional-culture
#10
Panpan Zhou, Zilin Liu, Xue Li, Bing Zhang, Xiaoyuan Wang, Jing Lan, Qing Shi, Dong Li, Xiuli Ju
While the conventional two-dimensional (2D) culture protocol is well accepted for the culture of mesenchymal stem cells (MSCs), this method fails to recapitulate the in vivo native three-dimensional (3D) cellular microenvironment, and may result in phenotypic changes, and homing and migration capacity impairments. MSC preparation in 3D culture systems has been considered an attractive preparatory and delivery method recently. We seeded human umbilical cord-derived MSCs (hUCMSCs) in a 3D culture system with porcine acellular dermal matrix (PADM), and investigated the phenotypic changes, the expression changes of some important receptors, including Toll-like receptors (TLRs) and C-X-C chemokine receptor type 4 (CXCR4) when hUCMSCs were transferred from 2D to 3D systems, as well as the alterations in in vivo homing and migration potential...
July 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28734116/preparing-platelet-rich-plasma-with-whole-blood-harvested-intraoperatively-during-spinal-fusion
#11
Bin Shen, Zheng Zhang, Ning-Feng Zhou, Yu-Feng Huang, Yu-Jie Bao, De-Sheng Wu, Ya-Dong Zhang
BACKGROUND Platelet-rich plasma (PRP) has gained growing popularity in use in spinal fusion procedures in the last decade. Substantial intraoperative blood loss is frequently accompanied with spinal fusion, and it is unknown whether blood harvested intraoperatively qualifies for PRP preparation. MATERIAL AND METHODS Whole blood was harvested intraoperatively and venous blood was collected by venipuncture. Then, we investigated the platelet concentrations in whole blood and PRP, the concentration of growth factors in PRP, and the effects of PRP on the proliferation and viability of human bone marrow-derived mesenchymal stem cells (HBMSCs)...
July 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28732434/effects-of-intraperitoneal-injection-of-allogeneic-bone-marrow-derived-mesenchymal-stem-cells-on-bronchiolitis-obliterans-in-mice-model
#12
Sakine Işık, Nevin Uzuner, Meral Karaman, Özkan Karaman, Müge Kıray, İlknur Kozanoğlu, Hüsnü Alper Bağrıyanık, Zeynep Arıkan-Ayyıldız, Melis Kartal Yandım, Yusuf Baran
Bone marrow-derived mesenchymal stem cells (BMSCs) can ameliorate a variety of lung diseases such as asthma, lung fibrosis, and acute lung injury by its anti-inflammatory and immunmodulatory effects. In this study, we developed a mouse model of bronchiolitis obliterans (BO) and evaluated the effects of the intraperitoneal administration of BMSCs on lung histopathology and cytokine levels. 25 BALB/c mice were divided into four groups; control group (Group I), BO developed and 1x106 BMSCs-injected group (Group II), non-BO, 1x106 BMSCs-injected group (Group III), and BO developed and saline-injected group (Group IV)...
June 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/28732358/lenalidomide-restores-the-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-from-multiple-myeloma-patients-via-deactivating-notch-signaling-pathway
#13
Juan Guo, Chengming Fei, Youshan Zhao, Sida Zhao, Qingqing Zheng, Jiying Su, Dong Wu, Xiao Li, Chunkang Chang
Multiple myeloma (MM) always presents osteolytic bone lesions, resulting from the abnormal osteoblastic and osteoclastic function in patients. MM patients exhibit the impairment of osteogenic differentiation of BMMSCs (bone marrow mesenchymal stem cells) and osteoblast deficiency. Effects of the drug, lenalidomide on the osteoblastic functions and the involved mechanisms remain unexplored. In the present study, it is observed that the osteogenic differentiation of BMMSCs from MM patients (MM-MSCs) is impaired and activation of Notch signaling pathway in MM-MSCs is abnormal...
July 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28731632/macrophages-of-different-phenotypes-influence-the-migration-of-bmscs-in-plga-scaffolds-with-different-pore-size
#14
Yuankun Dai, Xuguang Li, Ruihan Wu, Ying Jin, Changyou Gao
Immune modulation of the scaffolds not only reduces the host immunological rejection response, but also improves the regenerative cell migration into the scaffolds. Herein a convenient immune modulation of poly(lactide-co-glycolide) (PLGA) scaffold was applied with macrophages of different phenotypes to evaluate its influence on the migration behavior of bone marrow mesenchymal stem cells (BMSCs). With pro-inflammatory macrophages (M1) pre-loading, BMSCs migrated significantly faster into the PLGA scaffold, compared with those in the control scaffold or pre-seeded with inactivated macrophages (M0)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731172/comparison-of-the-neuronal-differentiation-abilities-of-bone-marrow%C3%A2-derived-and-adipose-tissue%C3%A2-derived-mesenchymal-stem-cells
#15
Yani Zheng, Chao Huang, Fang Liu, Haiyan Lin, Xiangqun Yang, Zhiying Zhang
Bone marrow‑derived mesenchymal stem cells (BMSCs) and adipose tissue‑derived mesenchymal stem cells (ADSCs) are able to differentiate into neuron‑like cells when exposed to small molecule compounds, however the specific differences in their neuronal differentiation abilities remain to be fully elucidated. The present study aimed to compare the neuronal differentiation abilities of BMSCs and ADSCs. BMSCs and ADSCs from the same Sprague Dawley rats were isolated and cultured for use. The proliferation capacity was revealed using a cell counting method...
July 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28730782/human-glioma-cells-conditioned-medium-promotes-proliferation-and-migration-of-human-bone-marrow-mesenchymal-stromal-cells-in-vitro
#16
Rusen Zhu, Xinggui Chen, Jinghong Li, Liubo Lan
PURPOSE: To investigate changes in cell proliferation and migration of human bone marrow-derived mesenchymal stromal cells (hMSCs) in glioma environment, in order to assess the tumorigenic risk of hMSCs in the clinical application to treat human gliomas. METHODS: hMSCs were obtained from normal adult persons and identified by their morphological characteristics and test of their stemness. The U251 glioma cell-conditioned medium (U251-CM) was obtained to simulate the human glioma environment in vitro...
May 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28729762/combination-of-bone-marrow-mesenchymal-stem-cells-and-cartilage-fragments-contribute-to-enhanced-repair-of-osteochondral-defects
#17
Mohammed Abbas
Cartilage tissue engineering using stem cells and biomaterials is considered a promising approach despite poor outcomes. We hypothesise that articular cartilage fragments provides native environmental cues to enhance stem cell differentiation. As such we evaluated the chondrogenic differentiation and repair of critical size defect in a human explant osteochondral model (OD) using bone marrow derived mesenchymal stem cells (BM-MSCs) and homogenised cartilage. BM-MSCs were established from the bone-marrow plugs of patients undergoing total knee arthroplasty and characterized...
2017: Bioinformation
https://www.readbyqxmd.com/read/28728472/the-identification-of-interferon-%C3%AE-as-a-key-supportive-factor-for-retinal-differentiation-of-murine-mesenchymal-stem-cells
#18
Barbora Hermankova, Jan Kossl, Eliska Javorkova, Pavla Bohacova, Michaela Hajkova, Alena Zajicova, Magdalena Krulova, Vladimir Holan
Retinal disorders represent the main cause of decreased quality of vision and even blindness worldwide. The loss of retinal cells causes irreversible damage of the retina and there are currently no effective treatment protocols for most retinal degenerative diseases. A promising approach for the treatment of retinal disorders is represented by stem cell-based therapy. The perspective candidates are mesenchymal stem cells (MSCs) which can differentiate into multiple cell types and produce a number of trophic and growth factors...
July 20, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28727740/genetically-engineered-suicide-gene-in-mesenchymal-stem-cells-using-a-tet-on-system-for-anaplastic-thyroid-cancer
#19
Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Anaplastic thyroid cancer (ATC) is the most aggressive malignancy of the thyroid, during which undifferentiated tumors arise from the thyroid follicular epithelium. ATC has a very poor prognosis due to its aggressive behavior and poor response to conventional therapies. Gene-directed enzyme/prodrug therapy using genetically engineered mesenchymal stromal cells (MSC) is a promising therapeutic strategy. The doxycycline (DOX)-controlled Tet inducible system is the most widely utilized regulatory system and could be a useful tool for therapeutic gene-based therapies...
2017: PloS One
https://www.readbyqxmd.com/read/28727618/clinical-study-of-bone-regeneration-by-conditioned-medium-from-mesenchymal-stem-cells-after-maxillary-sinus-floor-elevation
#20
Wataru Katagiri, Junna Watanabe, Naoto Toyama, Masashi Osugi, Kohei Sakaguchi, Hideharu Hibi
OBJECTIVE: This clinical study was undertaken to evaluate the safety of use of the secretome of bone marrow-derived mesenchymal stem cells (MSC-CM) for maxillary sinus floor elevation (SFE). MATERIALS AND METHODS: MSC-CM was prepared from conditioned medium from human bone marrow-derived MSCs. Six partially edentulous patients were enrolled in the study. MSC-CM was mixed with porous beta-tricalcium phosphate (β-TCP) and implanted in 4 patients (experimental group), whereas only β-TCP was implanted in the other 2 patients (control group)...
August 2017: Implant Dentistry
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