Read by QxMD icon Read


Ines van Dooren, Kenn Foubert, Sebastiaan Bijttebier, Mart Theunis, Stefaniya Velichkova, Magda Claeys, Luc Pieters, Vassiliki Exarchou, Sandra Apers
Stone diseases present a major health problem in the Western society, since both urinary and biliary stones occur with a relatively high prevalence of 10-12 % and 10-20 %, respectively, and demonstrate a high recurrence rate. At the moment treatment is mainly based on interventional procedures, or prophylactic and dissolution therapy. However, many of the current drugs cause severe side effects, and therefore, there is an increasing interest in natural medicines. At the moment no registered herbal medicinal products are available for treatment of gallstones...
October 24, 2016: Planta Medica
Elizabeth M Mudge, Ying Liu, Jensen A Lund, Paula N Brown
Suitably validated analytical methods that can be used to quantify medicinally active phytochemicals in natural health products are required by regulators, manufacturers, and consumers. Hawthorn (Crataegus) is a botanical ingredient in natural health products used for the treatment of cardiovascular disorders. A method for the quantitation of vitexin-2″-O- rhamnoside, vitexin, isovitexin, rutin, and hyperoside in hawthorn leaf and flower raw materials and finished products was optimized and validated according to AOAC International guidelines...
October 24, 2016: Planta Medica
Mírian Feliciano Costa, Tais Iara Jesus, Bruno Rafael Pereira Lopes, Célio Fernando Figueiredo Angolini, Abner Montagnolli, Lorraine de Paula Gomes, Gabriela Sterle Pereira, Ana Lucia Tasca Gois Ruiz, João Ernesto Carvalho, Marcos Nogueira Eberlin, Catarina Dos Santos, Karina Alves Toledo
BACKGROUND: Eugenia spp. are used in popular medicine in the treatment of pain, diabetes, intestinal disorders and cough. The aim of the work is to evaluate, ex vivo and in vivo, the anti-inflammatory activity of the hydroethanolic extracts of the leaves of Eugenia aurata (EA) and Eugenia punicifolia HBK (EP) upon neutrophils. METHODS: Ex vivo, isolated human neutrophils were sensitized by Eugenia extracts (0.1-1000 μg/mL) and stimulated by PMA. In these conditions, different neutrophil activities related to inflammatory process were measured: adhesion, degranulation and NET release...
October 22, 2016: BMC Complementary and Alternative Medicine
Lite Yang, Juan Yang, Bingjie Xu, Faqiong Zhao, Baizhao Zeng
In this paper, a novel molecularly imprinted composite film modified electrode was presented for rutin (RT) detection. The modified electrode was fabricated by electropolymerization of pyrrole on a graphene-multiwalled carbon nanotubes composite (G-MWCNTs) coated glassy carbon electrode in the presence of RT. The netlike G-MWCNTs composite, prepared by in situ hydrothermal process, had high conductivity and electrocatalytic activity. At the resulting MIP/G-MWCNTs/GCE electrode RT could produce a sensitive anodic peak in pH 1...
December 1, 2016: Talanta
Sajjad Karim, Jaudah A Al-Maghrabi, Hasan M A Farsi, Ahmad J Al-Sayyad, Hans-Juergen Schulten, Abdelbaset Buhmeida, Zeenat Mirza, Alaa A Al-Boogmi, Fai T Ashgan, Manal M Shabaad, Hend F NourEldin, Khalid B M Al-Ghamdi, Adel Abuzenadah, Adeel G A Chaudhary, Mohammed H Al-Qahtani
BACKGROUND: Renal cell carcinoma (RCC) is a seventh ranked malignancy with poor prognosis. RCC is lethal at metastatic stage as it does not respond to conventional systemic treatments, and there is an urgent need to find out promising novel biomarkers for effective treatment. The goal of this study was to evaluate the biomarkers that can be potential therapeutic target and predict effective inhibitors to treat the metastatic stage of RCC. METHODS: We conducted transcriptomic profiling to identify differentially expressed genes associated with RCC...
September 30, 2016: BMC Cancer
Saravanan Shanmugam, John Prakash Baby, Rahul Chandran, Sajeesh Thankarajan, Parimelazhagan Thangaraj
The present study was aimed to evaluate the nutritional, antioxidant properties and inhibition of the key enzyme such as α-amylase and α-glucosidase from the fruits of Maesa indica. The results revealed that M. indica fruits possess an enormous amount of protein (45.68 mg/g), carbohydrates (25.12 mg/g) and mineral elements. The acetone extract were capable of hunting radicals by providing electrons and break chain reaction, especially in ABTS(·+) (3719.23 µmol TE/g extract), OH(·) (66.50 %) and NO(·) (81...
July 2016: Journal of Food Science and Technology
Bingxian Yang, Qijie Guan, Jingkui Tian, Setsuko Komatsu
: High level of UV-B irradiation followed by dark treatment (HUV-B+D) causes accumulation of secondary metabolites in Clematis terniflora DC. To investigate the response mechanism under HUV-B+D, transcriptomic and proteomic analyses were performed in leaves of C. terniflora. The number of genes related to tetrapyrrole synthesis, amino acid metabolism, tricarboxylic acid cycle, and mitochondrial electron transport chains was hierarchically changed in leaves of C. terniflora under HUV-B+D...
October 17, 2016: Journal of Proteomics
Sagrario Martín-Aragón, Karim Lizeth Jiménez-Aliaga, Juana Benedí, Paloma Bermejo-Bescós
BACKGROUND: Plant secondary metabolites may induce adaptive cellular stress-responses in a variety of cells including neurons at the sub-toxic doses ingested by humans. Such 'neurohormesis' phenomenon, activated by flavonoids such as quercetin or rutin, may involve cell responses driven by modulation of signaling pathways which are responsible for its neuroprotective effects. PURPOSE: We attempt to explore the molecular mechanisms involved in the neurohormetic responses to quercetin and rutin exposure, in a SH-SY5Y cell line which stably overexpresses the amyloid precursor protein (APP) Swedish mutation, based on a biphasic concentration-response relationship for cell viability...
November 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Emmanuel Anyachukwu Irondi, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon
AIM: To evaluate the phenolics composition and inhibitory effect of the leaves extracts of Ocimum basilicum and Ocimum gratissimum on two key enzymes (pancreatic lipase [PL] and angiotensin 1-converting enzyme [ACE]) involved in obesity and hypertension in vitro. MATERIALS AND METHODS: The phenolics (flavonoids and phenolic acids) were quantified using high-performance liquid chromatography coupled with diode array detection. PL and ACE inhibitory effects; DPPH* and ABTS*(+) scavenging activities of the extracts were tested using spectrophotometric methods...
September 2016: Journal of Intercultural Ethnopharmacology
Ganiyu Oboh, Omodesola O Ogunruku, Sunday I Oyeleye, Tosin A Olasehinde, Ayokunle O Ademosun, Aline Augusti Boligon
This study investigated the inhibitory effects of phenolic-rich extracts from Clerodendrum volubile leaves on cholinergic [acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)] and monoaminergic [monoamine oxidase (MAO)] enzymes' activities and pro-oxidants [Fe(2+) and quinolinic acid-(QA)] induced lipid peroxidation in rats brain homogenates in vitro. Free phenolic extracts (FPE) and bound phenolic extracts (BPE) were obtained via solvent extraction, and the total phenol and flavonoid contents were evaluated...
October 18, 2016: Journal of Dietary Supplements
Jing Wang, Meizhu Zheng, Lina Chen, Zhiqiang Liu, Yuchi Zhang, Chun-Ming Liu, Shu Liu
Hydroxyl radicals are the most reactive free radical of human body, a strong contributor to tissue damage. In this study, liquid chromatography coupled to electrospray ionization mass spectrometry was applied to screen and identify hydroxyl radical scavengers from the total flavonoids of Ginkgo biloba leaves, and high-performance counter current chromatography was used to separate and isolate the active compounds. Furthermore, molecular devices was used to determine hydroxyl radical scavenging activities of the obtained hydroxyl radical scavengers and other flavonoids from Ginkgo biloba leaves...
October 18, 2016: Journal of Separation Science
Susoma Jannat, Md Yousof Ali, Hyeung-Rak Kim, Hyun Ah Jung, Jae Sue Choi
The aim of this study was to investigate the protective effects of juice powders from sweet orange [Citrus sinensis (L.) Osbeck], unshiu mikan (Citrus unshiu Marcow), and mini tomato (Solanum lycopersicum L.), and their major flavonoids, hesperidin, narirutin, and rutin in tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells. The increased reactive oxygen species and decreased glutathione levels observed in t-BHP-treated HepG2 cells were ameliorated by pretreatment with juice powders, indicating that the hepatoprotective effects of juice powders and their major flavonoids are mediated by induction of cellular defense against oxidative stress...
September 2016: Preventive Nutrition and Food Science
Me-Hea Park
The high metabolic rate of harvested asparagus spears (Asparagus officinalis L.) causes rapid deterioration. To extend shelf life, we investigated the effect of sucrose treatment on asparagus during storage. Asparagus spears were treated with 3%, 5%, and 10% sucrose and stored at 2 °C for 20 h. Cellular respiration decreased, but other processes were unaltered by exogenous sucrose. The overall appearance of asparagus treated with 3% sucrose and stored at 2 °C for 18 days was rated as good and excellent, unlike that of untreated spears...
October 14, 2016: Biochemical and Biophysical Research Communications
Sedar Karakurt
Expression of a drug and xenobiotic metabolizing enzymes, cytochrome P450s (CYPs), and antioxidant enzymes can be modulated by various factors. The flavonoid rutin was investigated for its anti-carcinogen and protective effects as well as modulatory action on CYPs and phase II enzymes in human hepatocellular carcinoma cells. Rutin inhibited proliferation of HEPG2 cells in a dose-dependent manner with the IC50 value of 52.7 μmol L-1 and invasion of HEPG2 cells (21.6 %, p = 0.0018) and colony formation of those invaded cells (57...
December 1, 2016: Acta Pharmaceutica
Fabíula Francisca Abreu, Ana Carla Araujó de Souza, Simone Aparecida Teixeira, Antônio Garcia Soares, Daiane Franco Teixeira, Rosilene Calazans Soares, Marilia Trindade Santana, Sandra Lauton Santos, Soraia Kátia Pereira Costa, Marcelo Nicolas Muscará, Enilton Aparecido Camargo
INTRODUCTION: Acute pancreatitis (AP) may be severe and cause hospitalization or death, and the available treatment is insufficient to control pancreatic inflammation and pain. Rutin is a natural flavonoid with the potential to treat AP via anti-inflammatory, antinociceptive and antioxidant activities. AIM: This study investigated the beneficial effects of rutin on experimental AP induced by L-arginine administration in mice. METHODS: The L-arginine-induced AP model was used in Swiss mice (n = 6-8)...
October 13, 2016: Free Radical Research
Milica Pantelić, Dragana Dabić Zagorac, Maja Natić, Uroš Gašić, Sonja Jović, Dragan Vujović, Jelena Popović Djordjević
In this study, grapes and corresponding wines of Merlot /No 022, 025 and 029/ and Cabernet Franc /No 02, 010 and 012/ clones (recently developed) were evaluated regarding the total phenolic content, total anthocyanin content, and radical scavenging activity, aiming to better understand their quality and market potential. The nineteen individual polyphenols were quantified in studied grape samples using UHPLC coupled to a triple-quadrupole mass spectrometer. The mother grapes and wines were used as the relevant standards...
2016: PloS One
Guizhen Li, Wha Seung Ahn, Kyung Ho Row
Different kinds of deep eutectic solvents based on choline chloride and ionic liquids based on 1-methylimidazole were used to modify hybrid molecularly imprinted polymers with the monomer γ-aminopropyltriethoxysilane-methacrylic and three templates (rutin, scoparone, and quercetin). The materials were adopted as solid-phase extraction packing agents, and were characterized by FTIR spectroscopy and field emission scanning electron microscopy. The hybrid molecularly imprinted polymers modified by deep eutectic solvents had high recoveries and a strong recognition of rutin, scoparone, and quercetin in Herba Artemisiae Scopariae than those modified by ionic liquids...
October 12, 2016: Journal of Separation Science
Li-Jing Lin, Xiao-Bing Huang, Zhen-Cheng Lv
BACKGROUND: Pteris vittata L. is rich in flavonoids which exhibit different bioactivities. In order to investigate the flavonoids components of P. vittata L., extracts of the plant were isolated by column chromatography on silica gel and Sephadex LH-20. RESULTS: Four flavonoids compounds were obtained, the structures were identified as kaempferol (1), quercetin (2), kaempferol-3-O-d-glucopyranoside (3) and rutin (4), respectively, on the basis of NMR spectroscopic analyses...
2016: SpringerPlus
Daniela Sumczynski, Eva Kotásková, Jana Orsavová, Pavel Valášek
Zizania aquatica L. was analysed for total flavonoids, phenolics and HPLC profile in both free and bound phenolic fractions. The highest content of flavonoids (378-455mgRE/kg) was detected in bound fractions, while the highest polyphenols content (1061-2988mgGAE/kg) was determined in free fractions. Additionally, predominant phenolics were identified. To understand the contribution of individual phenolics to an antioxidant activity their mutual correlations were evaluated. Regarding free flavonoids, the main contributors to an antioxidant activity (r>0...
March 1, 2017: Food Chemistry
Clive Metcalfe, Anjana Ramasubramoni, Giordano Pula, Matthew T Harper, Stuart J Mundell, Carmen H Coxon
Thioredoxin (Trx) is an oxidoreductase with important physiological function. Imbalances in the NADPH/thioredoxin reductase/thioredoxin system are associated with a number of pathologies, particularly cancer, and a number of clinical trials for thioredoxin and thioredoxin reductase inhibitors have been carried out or are underway. Due to the emerging role and importance of oxidoreductases for haemostasis and the current interest in developing inhibitors for clinical use, we thought it pertinent to assess whether inhibition of the NADPH/thioredoxin reductase/thioredoxin system affects platelet function and thrombosis...
2016: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"