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extended spectrum beta lactamase

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https://www.readbyqxmd.com/read/29149965/comparision-of-ureteral-stent-colonization-between-deceased-and-live-donor-renal-transplant-recipients
#1
M Sarier, D Seyman, S Tekin, I Duman, B Uygun, M Demir, E Kukul, A H Yavuz
BACKGROUND: The use of a ureteral stent can cause a urinary tract infection (UTI), although it reduces urologic complications. UTIs are associated with a higher rate of ureteral stent colonization (USC). The aim of this study was to compare USC in living and deceased donor renal transplant recipients. MATERIAL AND METHODS: We conducted a prospective study of 48 patients who underwent renal transplantation between January and December 2016. The stents were removed aseptically, the inner surface of proximal and distal ends of stents were irrigated with liquid culture medium, and then they were vortexed for bacteriological investigation...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29146039/clinical-features-and-outcomes-of-bacteraemia-due-to-oxa-48-like-carbapenemase-producing-klebsiella-pneumoniae-in-a-tertiary-hospital
#2
Ana Madueño, Jonathan González-García, María Del Mar Alonso Socas, María Antonia Miguel Gómez, María Lecuona
INTRODUCTION: Limited therapeutic options and high mortality make the management of OXA-48-like carbapenemase-producing Klebsiella pneumoniae (KPOXA-48) bacteraemia complicated. The aim of the study was to describe the clinical characteristics of KPOXA-48 bacteraemia between October 2013 and December 2016. MATERIAL AND METHODS: The variables to analyse were retrospectively collected from medical records. Carbapenemase production was confirmed by phenotypic and molecular methods...
November 13, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29140240/case-report-strongyloides-stercoralis-hyperinfection-in-a-patient-with-chronic-lymphocytic-leukemia
#3
Richelle Guerrero-Wooley, Ernesto Aranda-Aguirre, Wencheng Li, Aimee Wilkin, Elizabeth Palavecino
Strongyloides stercoralis is an intestinal nematode that can cause disseminated infection in an immunocompromised host. It is most commonly acquired in developing countries. It was previously a common infection in many parts of the United States, particularly in the Appalachian region, but is rarely identified currently. Here, we describe a patient born and raised in Appalachia, with no history of travel outside the United States, who presented with chronic lymphocytic leukemia and S. stercoralis hyperinfection characterized by acute respiratory failure, altered mental status, and extended-spectrum-beta-lactamase Klebsiella pneumoniae bacteremia...
November 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29139076/extended%C3%A2-spectrum%C3%A2-beta-lactamase-esbl-producing-bacteria-isolated-from-hospital-wastewaters-rivers-and-aquaculture-sources-in-nigeria
#4
Olawale Olufemi Adelowo, Serena Caucci, Omowunmi Abosede Banjo, Ozioma Chinyere Nnanna, Eunice Olubunmi Awotipe, Florence Bosede Peters, Obasola Ezekiel Fagade, Thomas U Berendonk
Untreated wastewater is a risk factor for the spread of antibiotic resistance in the environment. However, little is known about the contribution of untreated wastewater to the burden of antibiotic resistance in the Nigerian environment. In this study, a total of 143 ceftazidime-/cefpodoxime-resistant bacteria isolated from untreated wastewater and untreated wastewater-contaminated surface and groundwater in Nigeria were screened for extended-spectrum β-lactamase (ESBL) genes, integrons and integron gene cassettes by PCR...
November 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29138665/-epidemiological-profile-of-uropathogenic-enterobacteria-producing-extended-spectrum-beta-lactamases
#5
Mohammed Sbiti, Khalid Lahmadi, Lhoussaine Louzi
Urinary tract infections due to enterobacteria producing extended spectrum beta-lactamases (ESBL-E) constitute a infectious risk, a major therapeutic challenge and can even lead, in some cases, to a deadlock beacuse of their multi-resistance to antibiotics. This study aimed to determine the epidemiological profile of uropathogenic ESBL-E and to describe their current level of resistance to antibiotics for a better patient management according to local data. We conducted a retrospective study of all ESBL-E strains isolated from all cytobacteriogical testing of urine (CBEU) treated in the Microbiology Laboratory at the Military Hospital of Moulay Ismail, Meknes over a period of three years (from 1 January 2013 to 31 December 2015)...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/29138113/prevalence-of-extended-spectrum-beta-lactamase-and-carbapenemase-encoding-genes-in-poultry-feces-from-algeria-and-marseille-france
#6
Chabou Selma, Leulmi Hamza, Davoust Bernard, Aouadi Atef, Jean-Marc Rolain
OBJECTIVE: The aim of this study was to evaluate the prevalence of extended-spectrum beta lactamase (ESBLs) and carbapenemase-encoding genes in poultry from Algeria and Marseille, France. MATERIALS AND METHODS: The samples consisted of feces from broilers from France and Algeria between 2014 and 2015. DNA extraction and quantitative PCR were performed on 833 fecal samples to screen for the presence of ESBLs (TEM, SHV, CTX-M) and carbapenemase-encoding genes (NDM, OXA48, OXA58)...
November 11, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29133211/beta-lactam-resistance-among-enterobacteriaceae-in-cambodia-the-four-year-itch
#7
REVIEW
Yannick Caron, Rattanak Chheang, Puthea Nop, Soda Meng, Sébastien Boyer, Arnaud Tarantola, Alexandra Kerléguer
Although antibiotics are too often used inappropriately in Cambodia, few data have been published on antimicrobial resistance in this country. Epidemic dissemination and the transfer of resistance genes to other bacterial species put the population at risk. The aim of this study was to evaluate the frequency and characteristics of extended-spectrum beta-lactamase (ESBL) enterobacteriaceae (ESBL-E) isolated in consecutive samples tested at Institut Pasteur du Cambodge (IPC) over a four-year period (2012-2015)...
November 10, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29127502/piperacillin-tazobactam-as-alternative-to-carbapenems-for-icu-patients
#8
REVIEW
Benoit Pilmis, Vincent Jullien, Alexis Tabah, Jean-Ralph Zahar, Christian Brun-Buisson
Several studies suggest that alternatives to carbapenems, and particulary beta-lactam/beta-lactamase inhibitor combinations, can be used for therapy of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE)-related infections in non-ICU patients. Little is known concerning ICU patients in whom achieving the desired plasmatic pharmacokinetic/pharmacodynamic (PK/PD) target may be difficult. Also, in vitro susceptibility to beta-lactamase inhibitors might not translate into clinical efficacy. We reviewed the recent clinical studies examining the use of BL/BLI as alternatives to carbapenems for therapy of bloodstream infection, PK/PD data and discuss potential ecological benefit from avoiding the use of carbapenems...
November 10, 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/29125052/prevalence-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-bloodstream-isolates-of-klebsiella-pneumoniae-in-a-tertiary-care-hospital
#9
Henrietta Abodakpi, Kai-Tai Chang, Ana María Sánchez Díaz, Rafael Cantón, Todd M Lasco, Katrina Chan, Amelia K Sofjan, Vincent H Tam
To improve prescribing of empiric therapy, the local molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) and Klebsiella pneumoniae carbapenemases (KPCs) in bloodstream isolates of K. pneumoniae were evaluated. Isolates resistant to third generation cephalosporins were screened phenotypically for ESBLs and carbapenemases, and subsequently confirmed by PCR for the presence of ESBL (blaTEM, blaSHV and blaCTX-M) and carbapenemase (blaKPC, blaVIM, blaNDM and blaOXA-48) genes. Hydrolytic activity (functional gene expression) was quantified using a nitrocefin degradation assay and correlated to ceftazidime or meropenem MIC...
November 10, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29116290/drug-resistance-ampc-%C3%AE-lactamase-and-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-isolated-from-fish-and-shrimp
#10
Marília Viana Albuquerque de Almeida, Ítalo Mendes Cangussú, Antonia Leonadia Siqueira de Carvalho, Izabelly Linhares Ponte Brito, Renata Albuquerque Costa
The present study aims to detect the production of extended-spectrum beta-lactamases (ESBL) by enterobacteria isolated from samples of fresh shrimp and fish obtained from the retail trade of the city of Sobral, Ceará State, Brazil. All bacterial isolates were submitted to identification and antimicrobial susceptibility testing using aminopenicillin, beta-lactamase inhibitors, carbapenem, 1st, 2nd, 3rd and 4th generation cephalosporins, and monobactam. Three types of beta-lactamases - ESBL, AmpC and KPC - were investigated...
November 6, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/29116010/multi-drug-resistance-and-extended-spectrum-beta-lactamase-producing-gram-negative-bacteria-from-chicken-meat-in-bharatpur-metropolitan-nepal
#11
Anil Shrestha, Anup Muni Bajracharya, Hemraj Subedi, Raju Shah Turha, Sachin Kafle, Saroj Sharma, Sunil Neupane, Dhiraj Kumar Chaudhary
OBJECTIVE: Multidrug resistance (MDR) and extended spectrum beta lactamase (ESBL) producer Gram negative bacteria are considered as a major health problem, globally. ESBL enzyme hydrolyses the beta lactam ring of third generation cephalosporins, which alters the structure of the antibiotic. Due to the modification in structure of the antibiotic, bacteria show resistance to these antibiotics. Resistant bacterial strains are transmitted to humans from animals through consumption of uncooked meat, through contact with uncooked meat and meat surfaces...
November 7, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29106325/diarrheagenic-escherichia-coli-and-shigella-with-high-rate-of-extended-spectrum-beta-lactamase-production-two-predominant-etiological-agents-of-acute-diarrhea-in-shiraz-iran
#12
Maneli Aminshahidi, Amir Arastehfar, Gholamreza Pouladfar, Esmayil Arman, Fereshteh Fani
This study was conducted to find the etiology of acute diarrhea in Iranian children and determine the antimicrobial resistance patterns. The pathogenic bacteria were recovered from 110/269 (40.9%) diarrheal fecal samples with the following profiles: the most predominant pathogen was diarrheagenic Escherichia coli (DEC) (43.6%), comprising enteroaggregative E. coli (23.6%), enteropathogenic E. coli (10.9%), enteroinvasive E. coli (5.5%), and enterotoxigenic E. coli (3.6%); Shigella spp. (37.3%), Salmonella spp...
November 6, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29102989/universal-or-targeted-approach-to-prevent-the-transmission-of-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-in-intensive-care-units-a-cost-effectiveness-analysis
#13
Lidia Kardaś-Słoma, Jean-Christophe Lucet, Anne Perozziello, Camille Pelat, Gabriel Birgand, Etienne Ruppé, Pierre-Yves Boëlle, Antoine Andremont, Yazdan Yazdanpanah
OBJECTIVE: Several control strategies have been used to limit the transmission of multidrug-resistant organisms in hospitals. However, their implementation is expensive and effectiveness of interventions for the control of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) spread is controversial. Here, we aim to assess the cost-effectiveness of hospital-based strategies to prevent ESBL-PE transmission and infections. DESIGN: Cost-effectiveness analysis based on dynamic, stochastic transmission model over a 1-year time horizon...
November 3, 2017: BMJ Open
https://www.readbyqxmd.com/read/29095871/raw-pet-food-as-a-risk-factor-for-shedding-of-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-in-household-cats
#14
Valérie O Baede, Els M Broens, Mirlin P Spaninks, Arjen J Timmerman, Haitske Graveland, Jaap A Wagenaar, Birgitta Duim, Joost Hordijk
BACKGROUND: Close contact between pets and owners provides the opportunity for transmission of antimicrobial resistant organisms like extended-spectrum beta-lactamase (ESBL)/AmpC beta-lactamase (AmpC)-producing Enterobacteriaceae, posing a risk to public health. OBJECTIVES: To investigate whether raw feed is a risk factor for household cats to shed ESBL-producing Enterobacteriaceae, a cohort study was designed. Additionally, raw and non-raw commercial pet food products were screened for the presence of ESBL-producing Enterobacteriaceae...
2017: PloS One
https://www.readbyqxmd.com/read/29090981/characterization-of-unexpressed-extended-spectrum-beta-lactamase-genes-in-antibiotic-sensitive-klebsiella-pneumoniae-isolates
#15
Zhao Zhang, Yao Zhai, Yatao Guo, Daixi Li, Zhanwei Wang, Jing Wang, Yusheng Chen, Qi Wang, Zhancheng Gao
OBJECTIVE: The current investigation explores whether extended-spectrum β-lactamase (ESBL) genes exist in clinical non-ESBL-producing Klebsiella pneumoniae isolates. METHODS: A total of 202 clinical isolates with non-ESBL-producing K. pneumoniae were collected from southern and middle of China. Thirteen β-lactamase genes (blaSHV, CTX-M, TEM, OXA-2, OXA-10, VEB, PER, SFO, GES, CSP, TLA, BEL, and IBC) were screened by PCR and their identity confirmed by sequencing of PCR products...
November 1, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29090680/vim-1-carbapenemase-producing-escherichia-coli-isolated-from-retail-seafood-germany-2016
#16
Nicole Roschanski, Sebastian Guenther, Thi Thu Tra Vu, Jennie Fischer, Torsten Semmler, Stephan Huehn, Thomas Alter, Uwe Roesler
Carbapenems belong to the group of last resort antibiotics in human medicine. Therefore, the emergence of growing numbers of carbapenemase-producing bacteria in food-producing animals or the environment is worrying and an important concern for the public health sector. In the present study, a set of 45 Enterobacteriaceae isolated from German retail seafood (clams and shrimps), sampled in 2016, were investigated by real-time PCR for the presence of carbapenemase-producing bacteria. One Escherichia coli (ST10), isolated from a Venus clam (Ruditapes philippinarum) harvested in the Mediterranean Sea (Italy), contained the carbapenemase gene blaVIM-1 as part of the variable region of a class I integron...
October 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29075483/extended-spectrum-and-metalo-beta-lactamase-producing-airborne-pseudomonas-aeruginosa-and-acinetobacter-baumanii-in-restricted-settings-of-a-referral-hospital-a-neglected-condition
#17
Fithamlak Bisetegen Solomon, Fiseha Wadilo, Efrata Girma Tufa, Meseret Mitiku
BACKGROUND: Frequently encountered multidrug-resistant bacterial isolates of P. aeruginosa and A. baumannii are common and prevalent in a hospital environment. The aim of this study was to determine the prevalence and pattern of antibiotic resistance, extended spectrum and metallo beta-lactamase producing P. aeruginosa and A. baumannii isolates from restricted settings of indoor air hospital environment. METHODS: A hospital-based cross-sectional study was conducted in Wolaita Sodo University Teaching and referral Hospital, Ethiopia from December 1/2015 to April 30/2015...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29067059/phenotypic-characterization-of-extended-spectrum-beta-lactamase-producing-e-coli-from-healthy-individuals-patients-sewage-sludge-cattle-chickens-and-raw-meat
#18
Rabia Saleem, Hasan Ejaz, Aizza Zafar, Sonia Younas, Ahsan Waheed Rathore
OBJECTIVE: The present study aimed to determine the frequency and antimicrobial profile of ESBL-producing isolates of E. coli in different environments. METHODS: This cross-sectional study was conducted at The Children's Hospital and The Institute of Child Health, Lahore from July to December 2015. The faecal specimens from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat (n = 122) were processed for microbiological analysis using MacConkey agar supplemented with cefotaxime...
July 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29064348/the-activity-of-fosfomycin-against-extended-spectrum-beta-lactamase-producing-isolates-of-enterobacteriaceae-recovered-from-urinary-tract-infections-a-single-center-study-over-a-period-of-12-years
#19
Parisa Aris, Mohammad Ali Boroumand, Mohammad Rahbar, Masoumeh Douraghi
Despite global efforts to tackle resistance in extended-spectrum beta-lactamase (ESBL)-producing isolates via old antibiotics, there are limited data on the efficacy of fosfomycin-an old oral antibiotic-against Enterobacteriaceae in the Middle East. The purpose of this study was to evaluate the in vitro activity of fosfomycin against urinary ESBL-producing isolates of Enterobacteriaceae. Between 2004 and 2015, 363 isolates of ESBL-producing Enterobacteriaceae were recovered from high-risk patients suffering from cardiac disorders and were subjected to polymerase chain reaction using specific primers for the blaTEM, blaSHV, and blaCTX-M genes...
October 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29063880/discriminatory-power-of-three-typing-techniques-in-determining-relatedness-of-nosocomial-klebsiella-pneumoniae-isolates-from-a-tertiary-hospital-in-india
#20
Swathi Purighalla, Sarita Esakimuthu, Mallika Reddy, George K Varghese, Vijay S Richard, Vasan K Sambandamurthy
PURPOSE: The purpose of this study was to evaluate the discriminatory power of two DNA-based technique and a protein-based technique for the typing of nosocomial isolates of Klebsiella pneumoniae. A second objective was to determine the antimicrobial susceptibility pattern and characterise the presence of genes encoding extended-spectrum beta-lactamases (ESBLs) and carbapenemases. MATERIALS AND METHODS: Forty-six K. pneumoniae isolates from patients with bloodstream infections at a tertiary care hospital in India between December 2014 and December 2015 were studied...
July 2017: Indian Journal of Medical Microbiology
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