Read by QxMD icon Read

Cerebrospinal fluid-contacting nucleus

Yan Fei, Xin Wang, Songsong Chen, Qiangqiang Zhou, Chao Zhang, Ying Li, Lihong Sun, Licai Zhang
Evidence has suggested that cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) is correlated with the development and recurrence of pain. A recent research showed that the CSF-contacting nucleus acts as a component of the descending 5-hydroxytryptamine (5-HT) system and plays a role in descending pain inhibition. However, limited studies are conducted to investigate the relationship between the CSF-contacting nucleus and pain. In present study, we explored the effect of CSF-contacting nucleus on nociceptive behaviors in both normal and neuropathic rats via targeted ablation of the CSF-contacting nucleus in the brainstem, using cholera toxin subunit B-saporin (CB-SAP), a cytotoxin coupled to cholera toxin subunit B...
July 2016: Neurochemical Research
Fang Zhou, Jia-You Wang, En-Qi Tian, Li-Cai Zhang
The present study was aimed to investigate the role of cerebrospinal fluid-contacting nucleus (CSF-CN) neurons in modulation of inflammatory pain and underlying mechanism. The inflammatory pain model was made by subcutaneous injection of the complete Freund's adjuvant (CFA) into the left hind paw of rats. The phosphorylation level of PKC (p-PKC) was examined by Western blot. Thermal withdrawal latency (TWL) of the rats was measured to assess inflammatory pain. The results showed that, compared with the sham controls, the inflammatory pain model rats showed shortened TWL on day 1, 3, and 7 after CFA injection, as well as increased level of p-PKC in CSF-CN neurons at 24 h after CFA injection...
December 25, 2015: Sheng Li Xue Bao: [Acta Physiologica Sinica]
Chun-Guang Wang, Si-Yuan Song, Yan-Ling Ding, Shu-Qin Guo, Xin Liu, Shi Hao, Xin Li, Na Chen, Yu Zhang, Li-Cai Zhang
BACKGROUND: The activation of mitogen-activated protein kinases (MAPKs) have been observed in synaptic plasticity processes of learning and memory in neuropathic pain. Cerebrospinal fluid-contacting nucleus (CSF-CN) has been identified with the onset and persistence of neuropathic pain. However, whether extracellular signal-regulated protein kinase 5 (ERK5), a member of MAPKs, in CSF-CN participates in neuropathic pain has not been studied yet. OBJECTIVE: The aim of the present study was to identify the role of ERK5 in CSF-CN on the formation and development of neuropathic pain, and to investigate its possible mechanism...
November 2015: Pain Physician
Yue-Hong Wu, Si-Yuan Song, He Liu, Dan Xing, Xin Wang, Yan Fei, Guang-Ling Li, Chao Zhang, Ying Li, Li-Cai Zhang
The contribution of the cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) and adrenomedullin (ADM) to the developmental modulation of stressful events remains controversial. This study explored the effects of endogenous ADM in the CSF-contacting nucleus on immobilization of stress-induced physiological parameter disorders and glucocorticoid hormone releasing hormone (CRH), rat plasma corticosterone expression, and verification of such effects by artificially lowering ADM expression in the CSF-contacting nucleus by targeted ablation of the nucleus...
June 2015: Neuropeptides
Dan Xing, Yuehong Wu, Guangling Li, Siyuan Song, Yuepeng Liu, He Liu, Xing Wang, Yan Fei, Chao Zhang, Ying Li, Licai Zhang
The brainstem plays an important role in controlling sodium and water homeostasis. It is a major regulatory site for autonomic and motor functions. Moreover, it integrates cerebrospinal fluid (CSF) signals with neuronal and hormonal signals. Evidence suggests that the CSF-contacting nucleus (CSF-CN) transmits and integrates CSF signals, but, the definitive role of CSF-CN in sodium homeostasis is poorly understood. In this study, we used c-Fos as a marker of neuronal activity and causing colocalization of Nax channel and 5-HT...
August 1, 2015: Physiology & Behavior
Guangling Li, Xianfu Lu, Suming Zhang, Qiangqiang Zhou, Licai Zhang
The cerebrospinal fluid-contacting nucleus (CSF-CN) has been demonstrated to be involved in neuropathic pain, but the underlying molecular mechanisms remain unclear. Previous work has shown that mTOR and ERK1/2 are important signaling pathways regulating neuropathic pain. However, studies on the interactions between these major pathways in neuropathic pain are very rare. Therefore, the purpose of this study is to determine whether mTOR and ERK1/2 exist in the CSF-CN and elucidate their alterations in neuropathic pain, especially, the crosstalk between them...
May 2015: Neurochemical Research
Ping Chen, Qing-Song Lin, Li-Cai Zhang
The purpose of this research is to explore the distribution and expression of MAP kinase phosphatase-1 (MKP-1) in cerebrospinal fluid (CSF)-contacting nucleus in depression, and provide experimental evidence to reveal the biological function and regulatory mechanisms of CSF-contacting nucleus in depression. Depression model was produced by chronic forced swimming stress (CFSS) in Sprague-Dawley (SD) rats. Intracerebroventricular injection of cholera toxin subunit B (CTb) labeled with horseradish peroxidase (CB-HRP) was used to specifically mark distal CSF-contacting nucleus...
February 25, 2015: Sheng Li Xue Bao: [Acta Physiologica Sinica]
Jing Cao, Tong Wu, Li-Cai Zhang
OBJECTIVE: The changes of pain threshold and expression of 5-hydroxytryptamine(5-HT) and c-Fos in spinal dorsal horn of rats were observed after targetedly damaged the cerebraspinal fluid-contacting nucleus (CSF-contacting nucleus) to provide experimental evidence for the mechanism of regulating pain CSF-contacting nucleus involved in. METHODS: Male adult SD rats were divided into control, sham, choleratoxin subunit B conjugated with horse-radish peroxidase (CB-HRP)and damage groups randomly...
May 2014: Chinese Journal of Applied Physiology
He Liu, Wei-Wei Yan, Xiao-Xing Lu, Xiu-Li Zhang, Jing-Qiu Wei, Xiao-Yu Wang, Tiao Wang, Tong Wu, Jing Cao, Cui-Jie Shao, Fang Zhou, Hong-Xing Zhang, Peng Zhang, Ting Zang, Xian-Fu Lu, Jun-Li Cao, Hai-Lei Ding, Li-Cai Zhang
The brainstem is well recognized as a critical site for integrating descending modulatory systems that both inhibit and facilitate pain at the level of the spinal cord. The cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) distributes and localizes in the ventral periaqueductal central gray of the brainstem. Although emerging lines of evidence suggest that the CSF-contacting nucleus may be closely linked to transduction and regulation of pain signals, the definitive role of the CSF-contacting nucleus in pain modulation remains poorly understood...
November 2014: Experimental Neurology
Tong Wu, Jing Cao, Li-Cai Zhang
The purpose of this research is to explore the distribution and expression of hyperpolarization-activated cyclic nucleotide-gated channels subtype 2 (HCN2) in cerebrospinal fluid (CSF)-contacting nucleus in neuropathic pain, and provide experimental evidence to reveal the biological function and regulation mechanisms of CSF-contacting nucleus in neuropathic pain. Neuropathic pain model was produced by chronic constriction injury (CCI) in Sprague-Dawley (SD) rats. Intracerebroventricular injection of cholera toxin subunit B (CTb) labeled with horseradish peroxidase (CB-HRP) was used to specifically mark distal CSF-contacting nucleus...
June 25, 2014: Sheng Li Xue Bao: [Acta Physiologica Sinica]
Yusuke Nakane, Takashi Yoshimura
Most vertebrates living outside the tropical zone show robust physiological responses in response to seasonal changes in photoperiod, such as seasonal reproduction, molt, and migration. The highly sophisticated photoperiodic mechanism in Japanese quail has been used to uncover the mechanism of seasonal reproduction. Molecular analysis of quail mediobasal hypothalamus (MBH) revealed that local thyroid hormone activation within the MBH plays a critical role in the photoperiodic response of gonads. This activation is accomplished by two gene switches: thyroid hormone-activating (DIO2) and thyroid hormone-inactivating enzymes (DIO3)...
2014: Frontiers in Neuroscience
Jinfeng Wang, Suming Zhang, Li Li, Licai Zhang
Studies have demonstrated that the cerebrospinal fluid-contacting nucleus (CSF-CN) is involved in neuropathic pain, but the underlying molecular mechanisms still largely remain obscure. Emerging evidence suggests that spinal Wnt5a plays a crucial role in regulation of chronic pain. However, little is known about the potential role of the supraspinal Wnt5a in the development of chronic pain. To investigate whether Wnt5a exists in the CSF-CN and its role in neuropathic pain, double-labeled immunofluorescence staining was used to identify the expression of Wnt5a in the CSF-CN and western blot analysis of the CSF-CN was employed to verify the alteration of Wnt5a protein in the process of neuropathic pain...
February 2015: International Journal of Neuroscience
X Y Wang, W W Yan, X L Zhang, H Liu, L C Zhang
OBJECTIVE: The study explores the role of acid-sensitive ion channel 3 (ASIC3) in the cerebrospinal fluid (CSF)-contacting nucleus of the parenchyma of rat brain, in inflammatory pain. METHODS: We injected rats subcutaneously with complete Freund's adjuvant (CFA) into the plantar surface of the left hind paw. We then measured the thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT). After horseradish peroxidase-conjugated toxin subunit B (CB-HRP) retrograde tracing of one of the rats' lateral ventricles, we used immunohistochemistry, to observe the distribution and expression of ASIC3 in CSF-contacting nucleus with double labeling of CB-HRP and ASIC3...
March 2014: Neurological Research
L Dănăilă
BACKGROUND: The lateral ventricles are located in the center of the brain. Each ventricle lies in contact with five critical neural structures: the caudate nucleus, the thalamus, the fornix, the corpus callosum, and the genu of internal capsule.The authors report their experience in primary tumors of the lateral ventricles of the brain by analysing the symptomatology,the surgical treatment, the complications and the postoperative results. OBJECTIVE: To determine the importance of the surgical technique on the morbidity and the recurrence of lateral ventricles tumors...
September 2013: Chirurgia
María Rita Pérez, Elisabeth Pellegrini, Joel Cano-Nicolau, Marie-Madeleine Gueguen, Dounia Menouer-Le Guillou, Yohann Merot, Colette Vaillant, Gustavo M Somoza, Olivier Kah
In non-mammalian vertebrates, serotonin (5-HT)-producing neurons exist in the paraventricular organ (PVO), a diencephalic structure containing cerebrospinal fluid (CSF)-contacting neurons exhibiting 5-HT or dopamine (DA) immunoreactivity. Because the brain of the adult teleost is known for its neurogenic activity supported, for a large part, by radial glial progenitors, this study addresses the origin of newborn 5-HT neurons in the hypothalamus of adult zebrafish. In this species, the PVO exhibits numerous radial glial cells (RGCs) whose somata are located at a certain distance from the ventricle...
November 2013: European Journal of Neuroscience
E Sendemir, I M Kafa, H H Schäfer, G F Jirikowski
Sepsis is known to affect neuroendocrine circuits: injections of lipopolysaccaride are potent stimulators of oxytocin secretion from the posterior lobe, acute sepsis leads to uterus contractions and spontaneous abort. Here, we report changes in expression and distribution of hypothalamic oxytocin in rats that had been subjected to caecal ligation and puncture which led to acute sepsis. Septic animals showed loss of oxytocin immunostaining in perikarya of the supraoptic and paraventricular nuclei and an increase of oxytocin positive fibres, suggesting a shift of oxytocin pools into the axonal compartment...
September 2013: Journal of Chemical Neuroanatomy
Ramón Anadón, Isabel Rodríguez-Moldes, Fátima Adrio
The glycinergic cell populations in the brain of the lesser spotted dogfish were studied by a glycine immunofluorescence method. Numerous glycine-immunoreactive (Gly-ir) neurons were observed in different brain nuclei. In the telencephalon, Gly-ir cells were observed in the olfactory bulb, telencephalic hemispheres, and preoptic region. In the hypothalamus, cerebrospinal fluid-contacting Gly-ir neurons were observed in the lateral and posterior recess nuclei. Coronet cells of the saccus vasculosus were Gly-ir...
September 1, 2013: Journal of Comparative Neurology
Chun-Guang Wang, Yan-Ling Ding, Tian-Fang Zheng, Jing-Qiu Wei, He Liu, Yu-Feng Chen, Jia-You Wang, Li-Cai Zhang
The cerebrospinal fluid-contacting nucleus (CSF-CN) may influence actual composition of the CSF for non-synaptic signal transmission via releasing or absorbing bioactive substances, which distributes and localizes in the ventral periaqueductal central gray of the brainstem. Previous studies demonstrated that CSF-CN was involved in neuropathic pain and morphine dependence. Thus, to identify whether extracellular signal-regulated kinase 5 (ERK5) distributed in the CSF-CN and its function on the formation and development of morphine physical dependence, morphine withdrawal-like behavioral test and immunofluorescent technique were used in this research...
May 2013: Journal of Molecular Neuroscience: MN
Adeline Orts-Del'immagine, Nicolas Wanaverbecq, Catherine Tardivel, Vanessa Tillement, Michel Dallaporta, Jérôme Trouslard
Cerebrospinal fluid (CSF) contacting neurones have been observed in various brain regions such as the hypothalamus, the dorsal nucleus of the raphe and around the central canal (cc) of the spinal cord but their functional role remains unclear. At the level of the spinal cord, subependymal cerebrospinal fluid contacting neurones (S-CSF-cNs) present a peculiar morphology with a soma close to the ependymal layer, a process projecting towards the cc and ending with a bud and a cilium. These neurones were recently shown to express polycystin kidney disease 2-like 1 (PKD2L1 or TRPP3) channels that are members of the polycystin subtype of the transient receptor potential (TRP) channel superfamily and that have been proposed as either chemo- or mechanoreceptors in several tissues...
August 15, 2012: Journal of Physiology
Ting-ting Wu, Zi-jun Zhao, Chun Xu, Li-cai Zhang
To investigate the role of cerebrospinal fluid contacting nucleus (CSF-CN) and the changes of TRPC6 expression in morphine dependence and withdrawal. Male Sprague-Dawley rats (250 ± 50 g) were randomly divided into four groups: the normal group (N); the saline group (S); the morphine dependence group (D); the morphine withdrawal group (W). All animals in each group were tested the morphine withdrawal-like behavioral signs by total withdrawal scores. Double-labeled immunofluorescent technique and laser scanning confocal microscopy were used to identify the expression of TRPC6 in CSF-CN...
December 2011: Neurochemical Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"